Rw2G012220

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002238
Physical Location & Seq
Forward (+)
12646002 .. 12652209
6208 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G012220.1

Sequence Viewer

Length: 1164 bp
ATGATGGGAGGAAACTGCAGTAAAAACAAAGGTGTTGATGCTCCACCACCCCCACCGCCGCCGCCGCCACAACAACCCCTCGAAAGCAATACCAAATCCGAGTACACAGCCCATTTGAGCTCTTATGAGGCAGCCTGTAAGCTCGATTCGGGTTTACAATCGTTTGATGTATCTCTCCAACAGCGCACTAGCCGAGTCTTCAACTCACTTTCCACTGGAGTTGAAGTTAGGTCCCTATCTTTGGACTCACTCAAAGAGGTCACTGATTGTTTGCTTGAGATGAACCAGGAAGTGGTCAGTGTCATTCTCGAATGCAAGAAGGATGTATGGAACAATCAAGAGATGTTCTCTCTAGTGGAGGAGTACTTTGAGAACAGTCTCCAGACATTGGACTTCTGTACTGCCCTCGAGAGATGTCTTAAGCGTGCAGTGAACAATCAGCTGATAATTCAGGCTGCGGTGAGGCAGTTTGAGGAAGAAGTAGAAGCTGGTTTCGAAGGGAAGGGGTGTGTGAGGACATTGCAGGAGTTGAGGGCATTTAAGGCAGCCGGGGACCCTTTCACGGAGGAGTTCTTTGTACTATTTCAGGCAGTTTATAACCAACAGGCATCGATGCTCCAGAAATTGCAAGTCCAAAAGAGAAAGCTTGATAAGAAGTTGAAATCAGTGAAGGCTTGGCGGAAAGTGTCAAATGTTATTTTTGTTGCCACTTTTGTGTCTGTATTGATATTTTCGGTGGTGGCAGCTGCCATTGCTGCACCGCCACTTGTAACCGCCTTGGCTGGTGCACTGGCTGTTCCGATAGGTTCTGTTGGAAAATGGTGTAATATGCTTTGGAGCCGCTACGAGAGGGCTCTGAAAGGGCAGAGGGAGATAATAAGCTCAATGCAGGTTGGAAGTATGATTACAATGACAGACTTGAACAACATCAGGGTGCTTGTTGAGAAATTTGAAATCGAGATTGAGGCACTGCTTCAGAATGCTGACTTTGCTATTAGAGAAGAGGATGCTGTGAAGATCGTGATGATTGAGATCAACAAAAAGCTGGAAAAGTTTATGGAAACCATCGAAAGTTTAAGCCAAAACGCTGAGAAGTGTGGCCGGAATATAAGGAAGGCAAGGACAGTGATTTTGCAGAGAATAATTAGGCATCCCATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

387

Amino Acids

43.47

Weight (kDa)

7.99

Isoelectric Point (pI)

49.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 52 - 381 2.3e-131 Protein of unknown function (DUF677)
ROH1-like PF05633 53 - 160 6.1e-07 Protein ROH1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 597
Acc36I ACCTGC 1 cut(s) 880
AccB7I CCANNNNNTGG 2 cut(s) 292, 388
AciI CCGC 9 cut(s) 56, 59, 62, 65, 458, 679, 761, 774, 841
AcoI YGGCCR 1 cut(s) 1099
AcsI RAATTY 1 cut(s) 947
AcuI CTGAAG 1 cut(s) 959
AfaI GTAC 4 cut(s) 104, 365, 400, 579
AfiI CCNNNNNNNGG 4 cut(s) 241, 292, 388, 562
AflII CTTAAG 1 cut(s) 419
AgsI TTSAA 5 cut(s) 202, 224, 661, 922, 953
AjnI CCWGG 1 cut(s) 285
AleI CACNNNNGTG 1 cut(s) 713
AluBI AGCT 8 cut(s) 120, 142, 442, 488, 646, 746, 882, 1045
AluI AGCT 8 cut(s) 120, 142, 442, 488, 646, 746, 882, 1045
Alw21I GWGCWC 2 cut(s) 122, 790
Alw26I GTCTC 1 cut(s) 383
Alw44I GTGCAC 1 cut(s) 786
Ama87I CYCGRG 1 cut(s) 407
AoxI GGCC 1 cut(s) 1099
ApaLI GTGCAC 1 cut(s) 786
ApeKI GCWGC 6 cut(s) 131, 455, 545, 743, 746, 755
ApoI RAATTY 1 cut(s) 947
ArsI GACNNNNNNTTYG 2 cut(s) 1114, 1146
AspLEI GCGC 1 cut(s) 186
AspS9I GGNCC 2 cut(s) 231, 553
AsuC2I CCSGG 1 cut(s) 550
AsuHPI GGTGA 1 cut(s) 472
AsuII TTCGAA 1 cut(s) 495
AvaI CYCGRG 1 cut(s) 407
AvaII GGWCC 2 cut(s) 231, 553
BaeGI GKGCMC 1 cut(s) 790
BanII GRGCYC 2 cut(s) 122, 856
BarI GAAGNNNNNNTAC 2 cut(s) 889, 921
BbsI GAAGAC 1 cut(s) 190
Bbv12I GWGCWC 2 cut(s) 122, 790
BbvI GCAGC 6 cut(s) 143, 442, 557, 733, 742, 755
BccI CCATC 2 cut(s) 1073, 1163
BciT130I CCWGG 1 cut(s) 287
BcnI CCSGG 1 cut(s) 550
BcoDI GTCTC 1 cut(s) 383
BfaI CTAG 2 cut(s) 189, 353
BfmI CTRYAG 1 cut(s) 16
BfrI CTTAAG 1 cut(s) 419
BfuAI ACCTGC 1 cut(s) 880
BmcAI AGTACT 1 cut(s) 365
Bme1390I CCNGG 2 cut(s) 287, 550
Bme18I GGWCC 2 cut(s) 231, 553
BmeT110I CYCGRG 1 cut(s) 407
BmgT120I GGNCC 2 cut(s) 231, 553
BmiI GGNNCC 4 cut(s) 233, 554, 555, 839
BmrFI CCNGG 2 cut(s) 287, 550
BmsI GCATC 5 cut(s) 28, 603, 617, 997, 1159
BpiI GAAGAC 1 cut(s) 190
BplI GAGNNNNNCTC 2 cut(s) 332, 364
BpmI CTGGAG 3 cut(s) 237, 365, 602
Bpu14I TTCGAA 1 cut(s) 495
BpuEI CTTGAG 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 550
Bsa29I ATCGAT 1 cut(s) 611
BsaBI GATNNNNATC 1 cut(s) 1031
BsaJI CCNNGG 2 cut(s) 549, 777
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bsc4I CCNNNNNNNGG 4 cut(s) 241, 292, 388, 562
Bse1I ACTGG 2 cut(s) 220, 795
Bse3DI GCAATG 2 cut(s) 518, 750
Bse8I GATNNNNATC 1 cut(s) 1031
BseBI CCWGG 1 cut(s) 287
BseCI ATCGAT 1 cut(s) 611
BseDI CCNNGG 2 cut(s) 549, 777
BseGI GGATG 3 cut(s) 328, 1012, 1150
BseJI GATNNNNATC 1 cut(s) 1031
BseLI CCNNNNNNNGG 4 cut(s) 241, 292, 388, 562
BseMI GCAATG 2 cut(s) 518, 750
BseMII CTCAG 1 cut(s) 1080
BseNI ACTGG 2 cut(s) 220, 795
BseRI GAGGAG 2 cut(s) 374, 581
BseSI GKGCMC 1 cut(s) 790
BseXI GCAGC 6 cut(s) 143, 442, 557, 733, 742, 755
BsgI GTGCAG 2 cut(s) 447, 741
BshFI GGCC 1 cut(s) 1101
BshVI ATCGAT 1 cut(s) 611
BsiHKAI GWGCWC 2 cut(s) 122, 790
BsiHKCI CYCGRG 1 cut(s) 407
BsiSI CCGG 2 cut(s) 549, 1102
BslFI GGGAC 2 cut(s) 217, 566
BslI CCNNNNNNNGG 4 cut(s) 241, 292, 388, 562
BsmAI GTCTC 1 cut(s) 383
BsmFI GGGAC 2 cut(s) 217, 566
BsmI GAATGC 2 cut(s) 317, 985
BsnI GGCC 1 cut(s) 1101
BsoBI CYCGRG 1 cut(s) 407
Bsp119I TTCGAA 1 cut(s) 495
Bsp1286I GDGCHC 3 cut(s) 122, 790, 856
Bsp143I GATC 2 cut(s) 1017, 1032
BspACI CCGC 9 cut(s) 56, 59, 62, 65, 458, 679, 761, 774, 841
BspANI GGCC 1 cut(s) 1101
BspCNI CTCAG 1 cut(s) 1081
BspDI ATCGAT 1 cut(s) 611
BspLI GGNNCC 4 cut(s) 233, 554, 555, 839
BspMAI CTGCAG 1 cut(s) 20
BspMI ACCTGC 1 cut(s) 880
BspT104I TTCGAA 1 cut(s) 495
BspTI CTTAAG 1 cut(s) 419
BsrDI GCAATG 2 cut(s) 518, 750
BsrI ACTGG 2 cut(s) 220, 795
BssECI CCNNGG 2 cut(s) 549, 777
BssMI GATC 2 cut(s) 1017, 1032
BssT1I CCWWGG 1 cut(s) 777
Bst2UI CCWGG 1 cut(s) 287
Bst4CI ACNGT 2 cut(s) 377, 1126
Bst6I CTCTTC 1 cut(s) 996
BstAFI CTTAAG 1 cut(s) 419
BstBI TTCGAA 1 cut(s) 495
BstC8I GCNNGC 1 cut(s) 426
BstDEI CTNAG 1 cut(s) 1089
BstF5I GGATG 3 cut(s) 328, 1012, 1150
BstHHI GCGC 1 cut(s) 186
BstKTI GATC 2 cut(s) 1020, 1035
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 2 cut(s) 1017, 1032
BstMWI GCNNNNNNNGC 5 cut(s) 64, 542, 752, 755, 989
BstNI CCWGG 1 cut(s) 287
BstSCI CCNGG 2 cut(s) 285, 548
BstSFI CTRYAG 1 cut(s) 16
BstSLI GKGCMC 1 cut(s) 790
BstV1I GCAGC 6 cut(s) 143, 442, 557, 733, 742, 755
BstV2I GAAGAC 1 cut(s) 190
Bsu15I ATCGAT 1 cut(s) 611
BsuRI GGCC 1 cut(s) 1101
BsuTUI ATCGAT 1 cut(s) 611
BtsCI GGATG 3 cut(s) 328, 1012, 1150
BtsI GCAGTG 2 cut(s) 435, 968
BtsIMutI CAGTG 8 cut(s) 213, 261, 304, 435, 672, 788, 968, 1131
BveI ACCTGC 1 cut(s) 880
Cac8I GCNNGC 1 cut(s) 426
CfoI GCGC 1 cut(s) 186
Cfr13I GGNCC 2 cut(s) 231, 553
ClaI ATCGAT 1 cut(s) 611
Csp6I GTAC 4 cut(s) 103, 364, 399, 578
CspCI CAANNNNNGTGG 2 cut(s) 202, 237
CviQI GTAC 4 cut(s) 103, 364, 399, 578
DdeI CTNAG 1 cut(s) 1089
DpnI GATC 2 cut(s) 1019, 1034
DpnII GATC 2 cut(s) 1017, 1032
EaeI YGGCCR 1 cut(s) 1099
Eam1104I CTCTTC 1 cut(s) 996
EarI CTCTTC 1 cut(s) 996
EciI GGCGGA 1 cut(s) 694
Ecl136II GAGCTC 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 777
Eco24I GRGCYC 2 cut(s) 122, 856
Eco47I GGWCC 2 cut(s) 231, 553
Eco53kI GAGCTC 1 cut(s) 120
Eco57I CTGAAG 1 cut(s) 959
Eco88I CYCGRG 1 cut(s) 407
EcoICRI GAGCTC 1 cut(s) 120
EcoO109I RGGNCCY 2 cut(s) 231, 553
EcoRII CCWGG 1 cut(s) 285
EcoT14I CCWWGG 1 cut(s) 777
EcoT38I GRGCYC 2 cut(s) 122, 856
ErhI CCWWGG 1 cut(s) 777
FaiI YATR 7 cut(s) 126, 328, 597, 830, 902, 1058, 1109
FaqI GGGAC 2 cut(s) 217, 566
FokI GGATG 3 cut(s) 335, 1019, 1137
FriOI GRGCYC 2 cut(s) 122, 856
FspBI CTAG 2 cut(s) 189, 353
GlaI GCGC 1 cut(s) 185
GsuI CTGGAG 3 cut(s) 237, 365, 602
HaeIII GGCC 1 cut(s) 1101
HapII CCGG 2 cut(s) 549, 1102
HhaI GCGC 1 cut(s) 186
Hin6I GCGC 1 cut(s) 184
HinP1I GCGC 1 cut(s) 184
HindIII AAGCTT 1 cut(s) 644
HinfI GANTC 3 cut(s) 146, 195, 245
HpaII CCGG 2 cut(s) 549, 1102
HphI GGTGA 1 cut(s) 472
Hpy166II GTNNAC 4 cut(s) 105, 155, 433, 788
Hpy188I TCNGA 4 cut(s) 100, 801, 858, 978
Hpy188III TCNNGA 7 cut(s) 308, 338, 382, 409, 619, 958, 1021
Hpy8I GTNNAC 4 cut(s) 105, 155, 433, 788
HpyAV CCTTC 5 cut(s) 313, 491, 496, 664, 1108
HpyCH4III ACNGT 2 cut(s) 377, 1126
HpyCH4V TGCA 9 cut(s) 18, 315, 428, 523, 628, 758, 788, 889, 1135
HpyF10VI GCNNNNNNNGC 5 cut(s) 64, 542, 752, 755, 989
HpyF3I CTNAG 1 cut(s) 1089
HspAI GCGC 1 cut(s) 184
KflI GGGWCCC 1 cut(s) 553
Kzo9I GATC 2 cut(s) 1017, 1032
LmnI GCTCC 3 cut(s) 46, 621, 837
Lsp1109I GCAGC 6 cut(s) 143, 442, 557, 733, 742, 755
LweI GCATC 5 cut(s) 28, 603, 617, 997, 1159
MaeI CTAG 2 cut(s) 189, 353
MaeIII GTNAC 2 cut(s) 259, 769
MalI GATC 2 cut(s) 1019, 1034
MboI GATC 2 cut(s) 1017, 1032
MboII GAAGA 4 cut(s) 190, 488, 1013, 1027
MhlI GDGCHC 3 cut(s) 122, 790, 856
MluCI AATT 4 cut(s) 447, 623, 947, 1143
MlyI GAGTC 2 cut(s) 204, 239
MmeI TCCRAC 3 cut(s) 202, 793, 874
MseI TTAA 3 cut(s) 420, 540, 1076
MslI CAYNNNNRTG 2 cut(s) 713, 932
MspA1I CMGCKG 2 cut(s) 442, 746
MspCI CTTAAG 1 cut(s) 419
MspI CCGG 2 cut(s) 549, 1102
MspR9I CCNGG 2 cut(s) 287, 550
Mva1269I GAATGC 2 cut(s) 317, 985
MvaI CCWGG 1 cut(s) 287
MwoI GCNNNNNNNGC 5 cut(s) 64, 542, 752, 755, 989
NciI CCSGG 1 cut(s) 550
NdeII GATC 2 cut(s) 1017, 1032
NlaIV GGNNCC 4 cut(s) 233, 554, 555, 839
NmeAIII GCCGAG 1 cut(s) 218
NmuCI GTSAC 1 cut(s) 259
NspV TTCGAA 1 cut(s) 495
OliI CACNNNNGTG 1 cut(s) 713
PaeR7I CTCGAG 1 cut(s) 407
PctI GAATGC 2 cut(s) 317, 985
PfeI GAWTC 1 cut(s) 146
PflMI CCANNNNNTGG 2 cut(s) 292, 388
PleI GAGTC 2 cut(s) 203, 239
PpsI GAGTC 2 cut(s) 203, 239
PpuMI RGGWCCY 2 cut(s) 231, 553
PsiI TTATAA 1 cut(s) 597
Psp124BI GAGCTC 1 cut(s) 122
Psp5II RGGWCCY 2 cut(s) 231, 553
Psp6I CCWGG 1 cut(s) 285
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 4 cut(s) 233, 554, 555, 839
PspPI GGNCC 2 cut(s) 231, 553
PspPPI RGGWCCY 2 cut(s) 231, 553
PstI CTGCAG 1 cut(s) 20
PvuII CAGCTG 2 cut(s) 442, 746
RsaI GTAC 4 cut(s) 104, 365, 400, 579
RsaNI GTAC 4 cut(s) 103, 364, 399, 578
RseI CAYNNNNRTG 2 cut(s) 713, 932
SacI GAGCTC 1 cut(s) 122
SaqAI TTAA 3 cut(s) 420, 540, 1076
Sau3AI GATC 2 cut(s) 1017, 1032
Sau96I GGNCC 2 cut(s) 231, 553
ScaI AGTACT 1 cut(s) 365
SchI GAGTC 2 cut(s) 204, 239
ScrFI CCNGG 2 cut(s) 287, 550
SduI GDGCHC 3 cut(s) 122, 790, 856
SfaNI GCATC 5 cut(s) 28, 603, 617, 997, 1159
SfcI CTRYAG 1 cut(s) 16
Sfr274I CTCGAG 1 cut(s) 407
SfuI TTCGAA 1 cut(s) 495
SinI GGWCC 2 cut(s) 231, 553
SlaI CTCGAG 1 cut(s) 407
SmiMI CAYNNNNRTG 2 cut(s) 713, 932
SmlI CTYRAG 3 cut(s) 275, 407, 419
SmoI CTYRAG 3 cut(s) 275, 407, 419
Sse9I AATT 4 cut(s) 447, 623, 947, 1143
SsiI CCGC 9 cut(s) 56, 59, 62, 65, 458, 679, 761, 774, 841
SspMI CTAG 2 cut(s) 189, 353
SstI GAGCTC 1 cut(s) 122
StyD4I CCNGG 2 cut(s) 285, 548
StyI CCWWGG 1 cut(s) 777
TaaI ACNGT 2 cut(s) 377, 1126
TaqI TCGA 8 cut(s) 81, 144, 309, 408, 495, 611, 957, 1068
TasI AATT 4 cut(s) 447, 623, 947, 1143
TatI WGTACW 4 cut(s) 102, 363, 398, 577
TauI GCSGC 4 cut(s) 61, 64, 67, 843
TfiI GAWTC 1 cut(s) 146
Tru1I TTAA 3 cut(s) 420, 540, 1076
Tru9I TTAA 3 cut(s) 420, 540, 1076
TscAI CASTG 8 cut(s) 220, 268, 304, 435, 672, 795, 975, 1131
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 6 cut(s) 131, 455, 545, 743, 746, 755
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 1 cut(s) 296
TspGWI ACGGA 1 cut(s) 578
TspRI CASTG 8 cut(s) 220, 268, 304, 435, 672, 795, 975, 1131
Van91I CCANNNNNTGG 2 cut(s) 292, 388
Vha464I CTTAAG 1 cut(s) 419
VneI GTGCAC 1 cut(s) 786
VpaK11BI GGWCC 2 cut(s) 231, 553
XapI RAATTY 1 cut(s) 947
XhoI CTCGAG 1 cut(s) 407
XspI CTAG 2 cut(s) 189, 353
ZrmI AGTACT 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.