MD11G1180100.v1.1

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002238
Physical Location & Seq
Forward (+)
21887888 .. 21889318
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1180100.v1.1.491

Sequence Viewer

Length: 474 bp
ATGTCCCACTCAGTGGGATTCCAGCCTCCTTGCGATGAGTTCTATAGTAACGATCTTGAAGTTTTGGCAGCTGCCGTTGCTGCACCACTACTTGTAACGGCCTTGGCAGGAGCATTGGCTGTTCCAATAGGTTTCGTTGGAAAATGGTGTAATGTGCTGTGGAATAACTACGAGAGGAGGCTGAAAGGGCATAGGGAGATTGGAACGTTCGTCACAATGAAGGACCTGGACAACATCCGCGTTCTTGTAGACAAATTTGAAATTGGGATTGAATCGCTGTTGCACAATGCTGATTTTGCTTTTAGAGAAGAAAGCGTACCTGTGAAGCTAGTGATAGATCAGATCAAGAAAAAGCTTGATGTGTTTGTGGAAACCATTGATAATGTGAGCCAATATGCTGATAAGTGTAGCCGGAATATAAGGAAGGCAATGACAGTGATTTTGCAGAGATTAATCAGGCATCCCGACCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

158

Amino Acids

17.74

Weight (kDa)

6.42

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 21 - 150 6.5e-34 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 13
AccI GTMKAC 1 cut(s) 249
AccII CGCG 1 cut(s) 240
AciI CCGC 1 cut(s) 238
AclI AACGTT 1 cut(s) 206
AcsI RAATTY 1 cut(s) 254
AdeI CACNNNGTG 1 cut(s) 13
AfaI GTAC 1 cut(s) 318
AfiI CCNNNNNNNGG 1 cut(s) 13
AgsI TTSAA 3 cut(s) 59, 260, 272
AjnI CCWGG 1 cut(s) 225
AluBI AGCT 3 cut(s) 71, 328, 355
AluI AGCT 3 cut(s) 71, 328, 355
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 3 cut(s) 68, 71, 80
ApoI RAATTY 1 cut(s) 254
ArsI GACNNNNNNTTYG 2 cut(s) 424, 456
AseI ATTAAT 1 cut(s) 452
AspS9I GGNCC 1 cut(s) 223
AvaII GGWCC 1 cut(s) 223
BarI GAAGNNNNNNTAC 2 cut(s) 300, 332
BbvI GCAGC 3 cut(s) 58, 67, 80
BceAI ACGGC 2 cut(s) 59, 114
BciT130I CCWGG 1 cut(s) 227
BfaI CTAG 1 cut(s) 329
BfmI CTRYAG 1 cut(s) 43
BisI GCNGC 3 cut(s) 69, 72, 81
BlsI GCNGC 3 cut(s) 70, 73, 82
Bme1390I CCNGG 1 cut(s) 227
Bme18I GGWCC 1 cut(s) 223
BmgT120I GGNCC 1 cut(s) 223
BmrFI CCNGG 1 cut(s) 227
BmsI GCATC 1 cut(s) 469
BsaJI CCNNGG 1 cut(s) 102
Bsc4I CCNNNNNNNGG 1 cut(s) 13
Bse3DI GCAATG 1 cut(s) 435
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 1 cut(s) 102
BseGI GGATG 2 cut(s) 234, 460
BseLI CCNNNNNNNGG 1 cut(s) 13
BseMI GCAATG 1 cut(s) 435
BseMII CTCAG 1 cut(s) 24
BseRI GAGGAG 1 cut(s) 190
BseXI GCAGC 3 cut(s) 58, 67, 80
BsgI GTGCAG 1 cut(s) 66
Bsh1236I CGCG 1 cut(s) 240
BshFI GGCC 1 cut(s) 101
BsiSI CCGG 1 cut(s) 412
BslI CCNNNNNNNGG 1 cut(s) 13
BsnI GGCC 1 cut(s) 101
Bsp143I GATC 3 cut(s) 52, 337, 342
BspACI CCGC 1 cut(s) 238
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 1 cut(s) 23
BspFNI CGCG 1 cut(s) 240
BsrDI GCAATG 1 cut(s) 435
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 3 cut(s) 52, 337, 342
BssT1I CCWWGG 1 cut(s) 102
Bst2UI CCWGG 1 cut(s) 227
Bst4CI ACNGT 1 cut(s) 436
BstDEI CTNAG 1 cut(s) 10
BstF5I GGATG 2 cut(s) 234, 460
BstFNI CGCG 1 cut(s) 240
BstKTI GATC 3 cut(s) 55, 340, 345
BstMBI GATC 3 cut(s) 52, 337, 342
BstMWI GCNNNNNNNGC 4 cut(s) 77, 80, 187, 296
BstNI CCWGG 1 cut(s) 227
BstSCI CCNGG 1 cut(s) 225
BstSFI CTRYAG 1 cut(s) 43
BstUI CGCG 1 cut(s) 240
BstV1I GCAGC 3 cut(s) 58, 67, 80
BsuRI GGCC 1 cut(s) 101
BtgZI GCGATG 1 cut(s) 48
BtsCI GGATG 2 cut(s) 234, 460
BtsIMutI CAGTG 2 cut(s) 18, 441
Cfr13I GGNCC 1 cut(s) 223
Csp6I GTAC 1 cut(s) 317
CviJI RGCY 9 cut(s) 25, 71, 101, 119, 181, 328, 355, 390, 411
CviKI_1 RGCY 9 cut(s) 25, 71, 101, 119, 181, 328, 355, 390, 411
CviQI GTAC 1 cut(s) 317
DdeI CTNAG 1 cut(s) 10
DpnI GATC 3 cut(s) 54, 339, 344
DpnII GATC 3 cut(s) 52, 337, 342
DraIII CACNNNGTG 1 cut(s) 13
Eco130I CCWWGG 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 225
EcoT14I CCWWGG 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 102
FaiI YATR 4 cut(s) 45, 192, 396, 419
FblI GTMKAC 1 cut(s) 249
Fnu4HI GCNGC 3 cut(s) 69, 72, 81
FokI GGATG 2 cut(s) 221, 447
Fsp4HI GCNGC 3 cut(s) 69, 72, 81
FspBI CTAG 1 cut(s) 329
GluI GCNGC 3 cut(s) 69, 72, 81
HaeIII GGCC 1 cut(s) 101
HapII CCGG 1 cut(s) 412
HindIII AAGCTT 1 cut(s) 353
HinfI GANTC 2 cut(s) 18, 272
HpaII CCGG 1 cut(s) 412
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 1 cut(s) 342
Hpy188III TCNNGA 3 cut(s) 56, 346, 464
Hpy8I GTNNAC 1 cut(s) 250
HpyAV CCTTC 2 cut(s) 214, 418
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4IV ACGT 1 cut(s) 206
HpyCH4V TGCA 3 cut(s) 83, 283, 445
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 80, 187, 296
HpyF3I CTNAG 1 cut(s) 10
HpySE526I ACGT 1 cut(s) 206
Kzo9I GATC 3 cut(s) 52, 337, 342
LmnI GCTCC 1 cut(s) 110
LpnPI CCDG 7 cut(s) 35, 93, 212, 239, 333, 425, 442
Lsp1109I GCAGC 3 cut(s) 58, 67, 80
LweI GCATC 1 cut(s) 469
MaeI CTAG 1 cut(s) 329
MaeII ACGT 1 cut(s) 206
MaeIII GTNAC 3 cut(s) 47, 94, 211
MalI GATC 3 cut(s) 54, 339, 344
MboI GATC 3 cut(s) 52, 337, 342
MboII GAAGA 1 cut(s) 320
MluCI AATT 2 cut(s) 254, 261
MmeI TCCRAC 1 cut(s) 118
MnlI CCTC 3 cut(s) 36, 168, 171
MseI TTAA 1 cut(s) 452
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 1 cut(s) 412
MspR9I CCNGG 1 cut(s) 227
MvaI CCWGG 1 cut(s) 227
MvnI CGCG 1 cut(s) 240
MwoI GCNNNNNNNGC 4 cut(s) 77, 80, 187, 296
NdeII GATC 3 cut(s) 52, 337, 342
NmuCI GTSAC 1 cut(s) 211
PfeI GAWTC 2 cut(s) 18, 272
PflMI CCANNNNNTGG 1 cut(s) 13
PkrI GCNGC 3 cut(s) 70, 73, 82
PpuMI RGGWCCY 1 cut(s) 223
PshBI ATTAAT 1 cut(s) 452
Psp1406I AACGTT 1 cut(s) 206
Psp5II RGGWCCY 1 cut(s) 223
Psp6I CCWGG 1 cut(s) 225
PspGI CCWGG 1 cut(s) 225
PspPI GGNCC 1 cut(s) 223
PspPPI RGGWCCY 1 cut(s) 223
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 1 cut(s) 318
RsaNI GTAC 1 cut(s) 317
SaqAI TTAA 1 cut(s) 452
SatI GCNGC 3 cut(s) 69, 72, 81
Sau3AI GATC 3 cut(s) 52, 337, 342
Sau96I GGNCC 1 cut(s) 223
ScrFI CCNGG 1 cut(s) 227
SetI ASST 7 cut(s) 73, 133, 209, 228, 322, 330, 357
SfaNI GCATC 1 cut(s) 469
SfcI CTRYAG 1 cut(s) 43
SinI GGWCC 1 cut(s) 223
Sse9I AATT 2 cut(s) 254, 261
SsiI CCGC 1 cut(s) 238
SspMI CTAG 1 cut(s) 329
StyD4I CCNGG 1 cut(s) 225
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 1 cut(s) 436
TaiI ACGT 1 cut(s) 209
TasI AATT 2 cut(s) 254, 261
TfiI GAWTC 2 cut(s) 18, 272
Tru1I TTAA 1 cut(s) 452
Tru9I TTAA 1 cut(s) 452
TscAI CASTG 2 cut(s) 18, 441
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 3 cut(s) 68, 71, 80
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 1 cut(s) 233
TspRI CASTG 2 cut(s) 18, 441
Van91I CCANNNNNTGG 1 cut(s) 13
VpaK11BI GGWCC 1 cut(s) 223
VspI ATTAAT 1 cut(s) 452
XapI RAATTY 1 cut(s) 254
XmiI GTMKAC 1 cut(s) 249
XspI CTAG 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.