Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0002238

Physical Location & Seq

Genomic Coordinates Forward (+)

14614663 .. 14617405

Gene Structure 2743 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG163800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2298 bp

ATGTCTATGACTCTGAACTTTCTCGGAGGAGCACCTTTTCAACTGCTCTATGATGAGGTTAAGTTGGCCCTTAGCAATACCAGGAAGTTTAAAACCAACCTCAGAAGTCTCAAATTCACTCTAGATTGTTTACAGCCACAGATCATCGAACAGATTAGATTGCAGAACATGCAGCTGAATCTTGCCGTCCAAGAGATACAAGAACTTCAGAGGAAAATGGATGAGGGCATAGTGCTCATTGACCGGTTGTCGAGCCTCAGCACTTGGAACATCTGCATCTGGGGCGATTGCTGCAACTGCATGACGCCTGGTTATGCGGACCAGCTTGATGAGTTGAACGAATCTCTTAGACGTCTGCTTGAAATACTGAGGCTGCAGTCAATAGCGCAAGTCAACCAGGTCGTGATTTTGGTAAGGACAACAAGGGACAAACAAGATGAATTGGGGAAAAGACAGTTAGAGATTCTTAGCAACCAGCAATATATAATTGATGCGTTGAGAAAGCAGCAGCAAGTAGTAGAGAGGATCGAAAGGAATGGTGTTGCAGCGTCAACCGGAAGAGAAGGAGCTGCTCCTGCTCAGGTGTTGCACCTTGAAGCAGTATTTCAGGCGATATTTGATGTTGTTGTACAAGTAAAGATAGAAAATGATGTGAAGGAGGCCTTGGTTTTGGTAAGTAACTTAGAAAAGGAGATTGAGGAAATTGAAGGGAGTGGTGCGCTGCAAGGATATCATGTCCAAGGCGCTGCAGCTGAACCTCAATTGTCTAAGGTTGAATTGGATGTAATTAATATACAAGCCAGAAGTGATGTGAAGGAGTCTATAGATTCAGTAAGGAATGTAGAGGTGGGAGATGAAAGTCACAAGCGAATTGAAGGGAGTAGTCGGGCACCCCATCGAATTGATGTTGCAGTAGTTAAGCCTCCATCACCTACAATGGCATTAGATATAGTGAGCATTCCAGAGACATTGGATTCGGCAACAAACATGGAGGTGGGAGTCGAACAAACTGAAGGGACAGGGGAAAAAGATAGAGAAATATGGGTAGTCCCGTGTAAAGAAGCAGAGAAGGAAACCTCAACACCTACAGTTCCTGTTTTGGCTGCTCACAAAAGGCAAGCGTCAGTAGAAACACTTCTGAAAAATAACGAAGAAAACTATGCTGCTACAGGCGTTCAAGAAGCCCTTAGGAGTTCTGAAAAATTAGAGGTGGATTATACCAATATCAAATCCGAGTACACAGCCCATTTGAGCTCTTATGAGGCAGCCTGTAAGCTCGATTCAGGTTTACAATCGTTTGATGTATCTCTCCAACAGCGCACTAGCCGAGTCTTCAACTCCCTTTCCACTGGAGTAGAAGTTAGGTCCCTATCTTTGGACTCACTCAAAGAGGTCACTGATTGTTTGCTTGAGATGAACCAGGAAGTGGTCAGTGTCATTCTCGAATGCAAGGAGGATGTATGGAACAATCAAGAGATGTTCTCTCTAGTGGAGGAGTCCTTTGAGAACTGTCTCCAGACATTGGACTTCTGTACTGCCCTCGAGAGATGTCTCAAGCGTGCAGTGAACAATCAGCTGAAAATTCAGGTTGTGATGAGGCGGTTTGAGGAAGAAGTAGAAGCTGGTTTCGAAGGGAAGGGGTGTGTGAGGACATTGCAGGAGTTGAGGGCATTTAAGGCAGCCGGGGACCCTTTCACGGAGGAGTTCTTTGTACTATTTCAGGCAGTTTATAACCAACAGGCATCAATGCTCCAGAAATTGCTAGTCCGAAAGAGAAAGCTTGATAAGAAGTTGAAATCAGTGAAAGCTTGGCGGAAAGTGTCAAATGTTATTTTTGTTGCCACTTTTGTGTCTGTATTGATATTTTCGGTGGTGGCAGCTGCCATTGCTGCACCACCACTTGTAACCGCCTTGGCAGGTTCACTGGCTGTTCCAATAGGTTCTGTTGGAAAATGGTGTAATATGCTTTGGAGCCGCTTTGAGAGGGCTCTGAAAGGGCAGAGGGAGATAATAAGCTCAATGCAGGTTGGAAGTATGATTACAATGACAGACTTGAACAACATCAGGGTGCTTGTTGAGAAATTTGAAATCGAGATCGAGGCATTGCTGCAGAATGCTGACTTTGCTATTAGAGAAGAGGATGCTGTGAAGATAGTGATGATTGAGATCAACAAAAAGCTGGAAAAGTTTATGGAAACCATCGAAAGTTTAAGCCAAAACGCTGATAAGTGTGGCCGGGATATAAGGAAGGCAAGGACAGTGATTTTGAAGAGAATAATTAGGCATCCCATCAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF677

Protein Analysis

765

Amino Acids

86.05

Weight (kDa)

5.34

Isoelectric Point (pI)

50.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPW8	PF05659	10 - 135	6.8e-09	Arabidopsis broad-spectrum mildew resistance protein RPW8
DUF677	PF05055	430 - 759	1.5e-122	Protein of unknown function (DUF677)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002238)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18630 AT2G18630
fragaria_vesca	FvH4_1g13660 FvH4_1g13660 FvH4_1g13660 FvH4_1g13660 FvH4_1g13660 FvH4_1g13660 FvH4_1g13660 FvH4_1g13660 FvH4_1g13660 FvH4_1g13670 FvH4_1g14080 FvH4_1g14080 FvH4_1g14080 FvH4_1g14080 FvH4_1g14080 FvH4_1g14080 FvH4_1g14080
malus_domestica	MD02G1154800.v1.1 MD11G1180100.v1.1 MD15G1269200.v1.1
prunus_persica	Prupe.7G148500_v2.0.a1
pyrus_communis	pycom02g15720
rosa_chinensis	RchiOBHm_Chr2g0102731 RchiOBHm_Chr2g0102741
rosa_laevigata	RLG00000017176
rosa_multiflora	Rmu_co8294297.1_g000001 Rmu_sc0006537.1_g000037 Rmu_sc0006537.1_g000040
rosa_roxburghii	Rroxscaffold_2G00140350 Rroxscaffold_2G00140360
rosa_rugosa	Rorug02G0109300 Rorug02G0109300
rosa_samantha	Rh2AG157500 Rh2AG157600 Rh2BG164000 Rh2BG164100 Rh2CG163700 Rh2CG163800 Rh2DG162800 Rh2DG162900
rosa_wichuraiana	Rw2G012220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1731
AatII	GACGTC	1 cut(s)	355
Acc36I	ACCTGC	2 cut(s)	1907, 2014
AccB1I	GGYRCC	1 cut(s)	889
AccB7I	CCANNNNNTGG	2 cut(s)	1426, 1522
AciI	CCGC	5 cut(s)	317, 1600, 1813, 1908, 1975
AclWI	GGATC	1 cut(s)	533
AcoI	YGGCCR	1 cut(s)	2233
AcsI	RAATTY	3 cut(s)	113, 1581, 2081
AcuI	CTGAAG	2 cut(s)	191, 1032
AcyI	GRCGYC	2 cut(s)	305, 352
AfaI	GTAC	4 cut(s)	630, 1238, 1534, 1713
AfiI	CCNNNNNNNGG	4 cut(s)	1375, 1426, 1522, 1696
AgeI	ACCGGT	1 cut(s)	243
AhdI	GACNNNNNGTC	1 cut(s)	247
AjnI	CCWGG	4 cut(s)	80, 307, 396, 1419
AjuI	GAANNNNNNNTTGG	2 cut(s)	647, 679
AleI	CACNNNNGTG	1 cut(s)	1847
Alw21I	GWGCWC	3 cut(s)	34, 237, 1256
Alw26I	GTCTC	4 cut(s)	113, 959, 1517, 1556
AlwI	GGATC	1 cut(s)	533
AlwNI	CAGNNNCTG	1 cut(s)	1094
Ama87I	CYCGRG	1 cut(s)	1541
AoxI	GGCC	3 cut(s)	66, 660, 2233
ApoI	RAATTY	3 cut(s)	113, 1581, 2081
ArsI	GACNNNNNNTTYG	4 cut(s)	958, 990, 2248, 2280
AseI	ATTAAT	1 cut(s)	789
AsiGI	ACCGGT	1 cut(s)	243
Asp700I	GAANNNNTTC	1 cut(s)	1134
AspLEI	GCGC	4 cut(s)	388, 721, 746, 1320
AspS9I	GGNCC	4 cut(s)	67, 319, 1365, 1687
AsuC2I	CCSGG	2 cut(s)	1684, 2237
AsuHPI	GGTGA	1 cut(s)	921
AsuII	TTCGAA	1 cut(s)	1629
AvaI	CYCGRG	1 cut(s)	1541
AvaII	GGWCC	3 cut(s)	319, 1365, 1687
AxyI	CCTNAGG	1 cut(s)	1187
BaeGI	GKGCMC	1 cut(s)	892
BanI	GGYRCC	1 cut(s)	889
BanII	GRGCYC	2 cut(s)	1256, 1990
BarI	GAAGNNNNNNTAC	4 cut(s)	1119, 1151, 2023, 2055
BbsI	GAAGAC	1 cut(s)	1324
Bbv12I	GWGCWC	3 cut(s)	34, 237, 1256
BbvCI	CCTCAGC	1 cut(s)	257
BccI	CCATC	4 cut(s)	903, 934, 2207, 2297
BceAI	ACGGC	1 cut(s)	170
BciT130I	CCWGG	4 cut(s)	82, 309, 398, 1421
BcnI	CCSGG	2 cut(s)	1684, 2237
BcoDI	GTCTC	4 cut(s)	113, 959, 1517, 1556
BfaI	CTAG	4 cut(s)	122, 1323, 1487, 1763
BfmI	CTRYAG	6 cut(s)	374, 747, 822, 1086, 1167, 2108
BfoI	RGCGCY	1 cut(s)	747
BfuAI	ACCTGC	2 cut(s)	1907, 2014
Bme1390I	CCNGG	6 cut(s)	82, 309, 398, 1421, 1684, 2237
Bme18I	GGWCC	3 cut(s)	319, 1365, 1687
BmeRI	GACNNNNNGTC	1 cut(s)	247
BmeT110I	CYCGRG	1 cut(s)	1541
BmgT120I	GGNCC	4 cut(s)	67, 319, 1365, 1687
BmiI	GGNNCC	5 cut(s)	891, 1367, 1688, 1689, 1973
BmrFI	CCNGG	6 cut(s)	82, 309, 398, 1421, 1684, 2237
BmsI	GCATC	5 cut(s)	285, 481, 1751, 2131, 2293
BpiI	GAAGAC	1 cut(s)	1324
BplI	GAGNNNNNCTC	2 cut(s)	1466, 1498
BpmI	CTGGAG	3 cut(s)	1371, 1499, 1736
Bpu10I	CCTNAGC	3 cut(s)	71, 257, 579
Bpu14I	TTCGAA	1 cut(s)	1629
BpuEI	CTTGAG	2 cut(s)	1430, 1538
BpuMI	CCSGG	2 cut(s)	1684, 2237
BsaBI	GATNNNNATC	1 cut(s)	2165
BsaHI	GRCGYC	2 cut(s)	305, 352
BsaJI	CCNNGG	4 cut(s)	663, 739, 1683, 1911
BsaWI	WCCGGW	2 cut(s)	243, 554
BsaXI	ACNNNNNCTCC	2 cut(s)	1344, 1374
Bsc4I	CCNNNNNNNGG	4 cut(s)	1375, 1426, 1522, 1696
Bse118I	RCCGGY	1 cut(s)	243
Bse1I	ACTGG	2 cut(s)	1354, 1929
Bse21I	CCTNAGG	1 cut(s)	1187
Bse3DI	GCAATG	3 cut(s)	1652, 1884, 2102
Bse8I	GATNNNNATC	1 cut(s)	2165
BseBI	CCWGG	4 cut(s)	82, 309, 398, 1421
BseDI	CCNNGG	4 cut(s)	663, 739, 1683, 1911
BseGI	GGATG	5 cut(s)	226, 787, 1462, 2146, 2284
BseJI	GATNNNNATC	1 cut(s)	2165
BseLI	CCNNNNNNNGG	4 cut(s)	1375, 1426, 1522, 1696
BseMI	GCAATG	3 cut(s)	1652, 1884, 2102
BseMII	CTCAG	4 cut(s)	115, 271, 359, 593
BseNI	ACTGG	2 cut(s)	1354, 1929
BseRI	GAGGAG	3 cut(s)	42, 1508, 1715
BseSI	GKGCMC	1 cut(s)	892
BsgI	GTGCAG	2 cut(s)	1581, 1875
BshFI	GGCC	3 cut(s)	68, 662, 2235
BshNI	GGYRCC	1 cut(s)	889
BshTI	ACCGGT	1 cut(s)	243
BsiHKAI	GWGCWC	3 cut(s)	34, 237, 1256
BsiHKCI	CYCGRG	1 cut(s)	1541
BsiSI	CCGG	4 cut(s)	244, 555, 1683, 2236
BslFI	GGGAC	5 cut(s)	440, 1030, 1034, 1351, 1700
BslI	CCNNNNNNNGG	4 cut(s)	1375, 1426, 1522, 1696
BsmAI	GTCTC	4 cut(s)	113, 959, 1517, 1556
BsmFI	GGGAC	5 cut(s)	440, 1030, 1034, 1351, 1700
BsmI	GAATGC	3 cut(s)	957, 1451, 2119
BsnI	GGCC	3 cut(s)	68, 662, 2235
BsoBI	CYCGRG	1 cut(s)	1541
Bsp119I	TTCGAA	1 cut(s)	1629
Bsp1286I	GDGCHC	5 cut(s)	34, 237, 892, 1256, 1990
Bsp1407I	TGTACA	1 cut(s)	628
Bsp143I	GATC	4 cut(s)	141, 525, 2094, 2166
BspACI	CCGC	5 cut(s)	317, 1600, 1813, 1908, 1975
BspANI	GGCC	3 cut(s)	68, 662, 2235
BspCNI	CTCAG	4 cut(s)	114, 270, 360, 592
BspLI	GGNNCC	5 cut(s)	891, 1367, 1688, 1689, 1973
BspMAI	CTGCAG	3 cut(s)	378, 751, 2112
BspMI	ACCTGC	2 cut(s)	1907, 2014
BspPI	GGATC	1 cut(s)	533
BspT104I	TTCGAA	1 cut(s)	1629
BspT107I	GGYRCC	1 cut(s)	889
BsrDI	GCAATG	3 cut(s)	1652, 1884, 2102
BsrFI	RCCGGY	1 cut(s)	243
BsrGI	TGTACA	1 cut(s)	628
BsrI	ACTGG	2 cut(s)	1354, 1929
BssAI	RCCGGY	1 cut(s)	243
BssECI	CCNNGG	4 cut(s)	663, 739, 1683, 1911
BssMI	GATC	4 cut(s)	141, 525, 2094, 2166
BssNI	GRCGYC	2 cut(s)	305, 352
BssT1I	CCWWGG	3 cut(s)	663, 739, 1911
Bst2UI	CCWGG	4 cut(s)	82, 309, 398, 1421
Bst4CI	ACNGT	4 cut(s)	456, 1090, 1511, 2260
Bst6I	CTCTTC	3 cut(s)	553, 2130, 2264
BstACI	GRCGYC	2 cut(s)	305, 352
BstAPI	GCANNNNNTGC	1 cut(s)	169
BstAUI	TGTACA	1 cut(s)	628
BstBI	TTCGAA	1 cut(s)	1629
BstC8I	GCNNGC	2 cut(s)	1119, 1560
BstF5I	GGATG	5 cut(s)	226, 787, 1462, 2146, 2284
BstH2I	RGCGCY	1 cut(s)	747
BstHHI	GCGC	4 cut(s)	388, 721, 746, 1320
BstKTI	GATC	4 cut(s)	144, 528, 2097, 2169
BstMAI	GTCTC	4 cut(s)	113, 959, 1517, 1556
BstMBI	GATC	4 cut(s)	141, 525, 2094, 2166
BstMWI	GCNNNNNNNGC	9 cut(s)	169, 282, 291, 297, 575, 1676, 1886, 1889, 2123
BstNI	CCWGG	4 cut(s)	82, 309, 398, 1421
BstNSI	RCATGY	1 cut(s)	172
BstSCI	CCNGG	6 cut(s)	80, 307, 396, 1419, 1682, 2235
BstSFI	CTRYAG	6 cut(s)	374, 747, 822, 1086, 1167, 2108
BstSLI	GKGCMC	1 cut(s)	892
BstV2I	GAAGAC	1 cut(s)	1324
Bsu36I	CCTNAGG	1 cut(s)	1187
BsuRI	GGCC	3 cut(s)	68, 662, 2235
BtsCI	GGATG	5 cut(s)	226, 787, 1462, 2146, 2284
BtsI	GCAGTG	1 cut(s)	1569
BtsIMutI	CAGTG	7 cut(s)	1347, 1395, 1438, 1569, 1806, 1922, 2265
BveI	ACCTGC	2 cut(s)	1907, 2014
Cac8I	GCNNGC	2 cut(s)	1119, 1560
CaiI	CAGNNNCTG	1 cut(s)	1094
CfoI	GCGC	4 cut(s)	388, 721, 746, 1320
Cfr10I	RCCGGY	1 cut(s)	243
Cfr13I	GGNCC	4 cut(s)	67, 319, 1365, 1687
CseI	GACGC	3 cut(s)	313, 537, 1110
CsiI	ACCWGGT	1 cut(s)	396
Csp6I	GTAC	4 cut(s)	629, 1237, 1533, 1712
CspAI	ACCGGT	1 cut(s)	243
CviAII	CATG	4 cut(s)	169, 301, 734, 988
CviQI	GTAC	4 cut(s)	629, 1237, 1533, 1712
DpnI	GATC	4 cut(s)	143, 527, 2096, 2168
DpnII	GATC	4 cut(s)	141, 525, 2094, 2166
DraI	TTTAAA	1 cut(s)	91
DriI	GACNNNNNGTC	1 cut(s)	247
EaeI	YGGCCR	1 cut(s)	2233
Eam1104I	CTCTTC	3 cut(s)	553, 2130, 2264
Eam1105I	GACNNNNNGTC	1 cut(s)	247
EarI	CTCTTC	3 cut(s)	553, 2130, 2264
EciI	GGCGGA	1 cut(s)	1828
Ecl136II	GAGCTC	1 cut(s)	1254
Eco130I	CCWWGG	3 cut(s)	663, 739, 1911
Eco147I	AGGCCT	1 cut(s)	662
Eco24I	GRGCYC	2 cut(s)	1256, 1990
Eco32I	GATATC	1 cut(s)	731
Eco47I	GGWCC	3 cut(s)	319, 1365, 1687
Eco53kI	GAGCTC	1 cut(s)	1254
Eco57I	CTGAAG	2 cut(s)	191, 1032
Eco81I	CCTNAGG	1 cut(s)	1187
Eco88I	CYCGRG	1 cut(s)	1541
EcoICRI	GAGCTC	1 cut(s)	1254
EcoO109I	RGGNCCY	2 cut(s)	1365, 1687
EcoRII	CCWGG	4 cut(s)	80, 307, 396, 1419
EcoRV	GATATC	1 cut(s)	731
EcoT14I	CCWWGG	3 cut(s)	663, 739, 1911
EcoT38I	GRGCYC	2 cut(s)	1256, 1990
ErhI	CCWWGG	3 cut(s)	663, 739, 1911
FaeI	CATG	4 cut(s)	172, 304, 737, 991
FalI	AAGNNNNNCTT	4 cut(s)	647, 679, 1170, 1202
FaqI	GGGAC	5 cut(s)	440, 1030, 1034, 1351, 1700
FatI	CATG	4 cut(s)	168, 300, 733, 987
FokI	GGATG	5 cut(s)	233, 794, 1469, 2153, 2271
FriOI	GRGCYC	2 cut(s)	1256, 1990
FspBI	CTAG	4 cut(s)	122, 1323, 1487, 1763
GlaI	GCGC	4 cut(s)	387, 720, 745, 1319
GsuI	CTGGAG	3 cut(s)	1371, 1499, 1736
HaeII	RGCGCY	1 cut(s)	747
HaeIII	GGCC	3 cut(s)	68, 662, 2235
HapII	CCGG	4 cut(s)	244, 555, 1683, 2236
HgaI	GACGC	3 cut(s)	313, 537, 1110
HhaI	GCGC	4 cut(s)	388, 721, 746, 1320
Hin1I	GRCGYC	2 cut(s)	305, 352
Hin1II	CATG	4 cut(s)	172, 304, 737, 991
Hin6I	GCGC	4 cut(s)	386, 719, 744, 1318
HinP1I	GCGC	4 cut(s)	386, 719, 744, 1318
HincII	GTYRAC	2 cut(s)	394, 552
HindII	GTYRAC	2 cut(s)	394, 552
HindIII	AAGCTT	2 cut(s)	1778, 1806
HpaII	CCGG	4 cut(s)	244, 555, 1683, 2236
HphI	GGTGA	1 cut(s)	921
Hpy166II	GTNNAC	7 cut(s)	131, 394, 552, 1239, 1289, 1567, 1922
Hpy188I	TCNGA	9 cut(s)	15, 26, 104, 210, 1140, 1198, 1234, 1769, 1992
Hpy8I	GTNNAC	7 cut(s)	131, 394, 552, 1239, 1289, 1567, 1922
HpyCH4III	ACNGT	4 cut(s)	456, 1090, 1511, 2260
HpyCH4IV	ACGT	1 cut(s)	352
HpyF10VI	GCNNNNNNNGC	9 cut(s)	169, 282, 291, 297, 575, 1676, 1886, 1889, 2123
HpySE526I	ACGT	1 cut(s)	352
Hsp92I	GRCGYC	2 cut(s)	305, 352
Hsp92II	CATG	4 cut(s)	172, 304, 737, 991
HspAI	GCGC	4 cut(s)	386, 719, 744, 1318
KflI	GGGWCCC	1 cut(s)	1687
Kzo9I	GATC	4 cut(s)	141, 525, 2094, 2166
LmnI	GCTCC	5 cut(s)	29, 566, 577, 1755, 1971
LweI	GCATC	5 cut(s)	285, 481, 1751, 2131, 2293
MabI	ACCWGGT	1 cut(s)	396
MaeI	CTAG	4 cut(s)	122, 1323, 1487, 1763
MaeII	ACGT	1 cut(s)	352
MaeIII	GTNAC	4 cut(s)	677, 860, 1393, 1903
MalI	GATC	4 cut(s)	143, 527, 2096, 2168
MboI	GATC	4 cut(s)	141, 525, 2094, 2166
MboII	GAAGA	7 cut(s)	570, 1163, 1324, 1622, 2147, 2161, 2281
MfeI	CAATTG	1 cut(s)	761
MhlI	GDGCHC	5 cut(s)	34, 237, 892, 1256, 1990
MlyI	GAGTC	6 cut(s)	4, 827, 1008, 1338, 1373, 1505
MmeI	TCCRAC	3 cut(s)	1336, 1927, 2008
MroXI	GAANNNNTTC	1 cut(s)	1134
MseI	TTAA	6 cut(s)	60, 90, 789, 918, 1674, 2210
MslI	CAYNNNNRTG	3 cut(s)	992, 1847, 2066
MspA1I	CMGCKG	4 cut(s)	175, 752, 1576, 1880
MspI	CCGG	4 cut(s)	244, 555, 1683, 2236
MspR9I	CCNGG	6 cut(s)	82, 309, 398, 1421, 1684, 2237
MunI	CAATTG	1 cut(s)	761
Mva1269I	GAATGC	3 cut(s)	957, 1451, 2119
MvaI	CCWGG	4 cut(s)	82, 309, 398, 1421
MwoI	GCNNNNNNNGC	9 cut(s)	169, 282, 291, 297, 575, 1676, 1886, 1889, 2123
NciI	CCSGG	2 cut(s)	1684, 2237
NdeII	GATC	4 cut(s)	141, 525, 2094, 2166
NlaIII	CATG	4 cut(s)	172, 304, 737, 991
NlaIV	GGNNCC	5 cut(s)	891, 1367, 1688, 1689, 1973
NmeAIII	GCCGAG	1 cut(s)	1352
NmuCI	GTSAC	2 cut(s)	860, 1393
NspI	RCATGY	1 cut(s)	172
NspV	TTCGAA	1 cut(s)	1629
OliI	CACNNNNGTG	1 cut(s)	1847
PaeR7I	CTCGAG	1 cut(s)	1541
PceI	AGGCCT	1 cut(s)	662
PctI	GAATGC	3 cut(s)	957, 1451, 2119
PdmI	GAANNNNTTC	1 cut(s)	1134
PfeI	GAWTC	6 cut(s)	178, 341, 463, 827, 974, 1280
PflMI	CCANNNNNTGG	2 cut(s)	1426, 1522
PinAI	ACCGGT	1 cut(s)	243
PleI	GAGTC	6 cut(s)	4, 826, 1007, 1337, 1373, 1504
PpsI	GAGTC	6 cut(s)	4, 826, 1007, 1337, 1373, 1504
PpuMI	RGGWCCY	2 cut(s)	1365, 1687
PshBI	ATTAAT	1 cut(s)	789
PsiI	TTATAA	1 cut(s)	1731
Psp124BI	GAGCTC	1 cut(s)	1256
Psp5II	RGGWCCY	2 cut(s)	1365, 1687
Psp6I	CCWGG	4 cut(s)	80, 307, 396, 1419
PspGI	CCWGG	4 cut(s)	80, 307, 396, 1419
PspN4I	GGNNCC	5 cut(s)	891, 1367, 1688, 1689, 1973
PspPI	GGNCC	4 cut(s)	67, 319, 1365, 1687
PspPPI	RGGWCCY	2 cut(s)	1365, 1687
PstI	CTGCAG	3 cut(s)	378, 751, 2112
PstNI	CAGNNNCTG	1 cut(s)	1094
PvuII	CAGCTG	4 cut(s)	175, 752, 1576, 1880
RsaI	GTAC	4 cut(s)	630, 1238, 1534, 1713
RsaNI	GTAC	4 cut(s)	629, 1237, 1533, 1712
RseI	CAYNNNNRTG	3 cut(s)	992, 1847, 2066
SacI	GAGCTC	1 cut(s)	1256
SaqAI	TTAA	6 cut(s)	60, 90, 789, 918, 1674, 2210
Sau3AI	GATC	4 cut(s)	141, 525, 2094, 2166
Sau96I	GGNCC	4 cut(s)	67, 319, 1365, 1687
SchI	GAGTC	6 cut(s)	4, 827, 1008, 1338, 1373, 1505
ScrFI	CCNGG	6 cut(s)	82, 309, 398, 1421, 1684, 2237
SduI	GDGCHC	5 cut(s)	34, 237, 892, 1256, 1990
SexAI	ACCWGGT	1 cut(s)	396
SfaNI	GCATC	5 cut(s)	285, 481, 1751, 2131, 2293
SfcI	CTRYAG	6 cut(s)	374, 747, 822, 1086, 1167, 2108
Sfr274I	CTCGAG	1 cut(s)	1541
SfuI	TTCGAA	1 cut(s)	1629
SinI	GGWCC	3 cut(s)	319, 1365, 1687
SlaI	CTCGAG	1 cut(s)	1541
SmiMI	CAYNNNNRTG	3 cut(s)	992, 1847, 2066
SmlI	CTYRAG	3 cut(s)	1409, 1541, 1553
SmoI	CTYRAG	3 cut(s)	1409, 1541, 1553
SseBI	AGGCCT	1 cut(s)	662
SsiI	CCGC	5 cut(s)	317, 1600, 1813, 1908, 1975
SspMI	CTAG	4 cut(s)	122, 1323, 1487, 1763
SstI	GAGCTC	1 cut(s)	1256
StuI	AGGCCT	1 cut(s)	662
StyD4I	CCNGG	6 cut(s)	80, 307, 396, 1419, 1682, 2235
StyI	CCWWGG	3 cut(s)	663, 739, 1911
TaaI	ACNGT	4 cut(s)	456, 1090, 1511, 2260
TaiI	ACGT	1 cut(s)	355
TatI	WGTACW	4 cut(s)	628, 1236, 1532, 1711
TauI	GCSGC	1 cut(s)	1977
TfiI	GAWTC	6 cut(s)	178, 341, 463, 827, 974, 1280
Tru1I	TTAA	6 cut(s)	60, 90, 789, 918, 1674, 2210
Tru9I	TTAA	6 cut(s)	60, 90, 789, 918, 1674, 2210
TscAI	CASTG	7 cut(s)	1354, 1402, 1438, 1569, 1806, 1929, 2265
TseFI	GTSAC	2 cut(s)	860, 1393
Tsp45I	GTSAC	2 cut(s)	860, 1393
TspDTI	ATGAA	3 cut(s)	453, 870, 1430
TspGWI	ACGGA	1 cut(s)	1712
TspRI	CASTG	7 cut(s)	1354, 1402, 1438, 1569, 1806, 1929, 2265
Van91I	CCANNNNNTGG	2 cut(s)	1426, 1522
VpaK11BI	GGWCC	3 cut(s)	319, 1365, 1687
VspI	ATTAAT	1 cut(s)	789
XapI	RAATTY	3 cut(s)	113, 1581, 2081
XbaI	TCTAGA	1 cut(s)	121
XceI	RCATGY	1 cut(s)	172
XhoI	CTCGAG	1 cut(s)	1541
XmnI	GAANNNNTTC	1 cut(s)	1134
XspI	CTAG	4 cut(s)	122, 1323, 1487, 1763
ZraI	GACGTC	1 cut(s)	353

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.