RLG00000017176

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002238
Physical Location & Seq
Forward (+)
13778025 .. 13780420
2396 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017176

Sequence Viewer

Length: 1164 bp
ATGATGGGAGGAAACTGCAGTAAAAACAAAGGTGTTGATGCTCCACCACCCCCGCCACCGCCACAACAGCCCGTCGAAAACAATACCAAATCCGAGTACACAGCCCATTTGAGCTCTTATGAGGAAGCCTGTAAGCTCGATTCAGGTTTACAATCGTTTGATGTATCTCTCCAACAGCGCACAAGCCGAGTCTTCAACTCACTTTCCACTGGAGTTGAAGTTAGGTCCTTATCTTTGGACTCACTCAAAGAGGTCACTGATTGTTTGCTTGAGATGAACCAGGAAGTGGTCAGTGTCATTCTCGAATGCAAGAAGGATGTATGGAACAATCAAGAGATGTTCTCTCTAGTGGAGGAGTACTTTGAGAACAGTCTCCAGACATTGGACTTCTGTACTGCCCTCGAGAGATGTCTCAAGCGTGCAGTGAACAATCAGCTGATAATTCAGGCTGCGGTGAGGCGGTTTGAGGAAGAAGTAGAAGCTGGTTTCGAAGGGAAGGGGTGTGTGAGGACATTGCAGGAGTTGAGGGCATTTAAGGCAGCCAGGGACCCTTTCACGGAGGAGTTCTTTGTACTATTTCAGGCAGTTTATAACCAACAGGCATCGATGCTCCAGAAATTGCAAGTCCGGAAGAGAAAGCTTGATAAGAAGTTGAAATCAGTGAAAGCTTGGCGGAAAGTGTCAAATGTTATTTTTGTTGCCACTTTTGTGTCTGTATTGATATTTTCGGTGGTGGCAGCTGCGATTGCTGCACCGCCACTTGTAACTGCCTTGGCAGGTGCACTGGCTGTTCCGATAGGTTCTGTTGGAAAATGGTGTAATATGCTTTGGAGCCGGTACGAGAGGGCTCTGAAAGGGCAGAGGGAGATAATAAGCTCAATGCAGGTTGGAAGTATGATTACAATGACGGACTTGAACAACATCAGGGTGCTTGTTGAGAAATTTGAAATCGAGATTGAGGCACTGCTTCAGAATGCTGACTTTGCTATTAGAGAAGAGGATGCTGTGAAGATCGTGATGATTGAGATCAACAAAAAGCTGGAAAAGTTTATGGAAACCATCGAAAGTTTAAGCCAAAATGCTGATAACCATTTGGTAGAGATGGAGATTGTTTCTCTTCAGTCTGATACATTGCGGCTATATGCTCCCAATGAAGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

388

Amino Acids

43.64

Weight (kDa)

5.18

Isoelectric Point (pI)

51.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 50 - 364 1.7e-122 Protein of unknown function (DUF677)
ROH1-like PF05633 50 - 158 5.9e-07 Protein ROH1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 591
AarI CACCTGC 1 cut(s) 767
Acc36I ACCTGC 2 cut(s) 767, 874
AccB7I CCANNNNNTGG 2 cut(s) 286, 382
AccIII TCCGGA 1 cut(s) 627
AciI CCGC 7 cut(s) 53, 59, 452, 460, 673, 755, 1135
AcsI RAATTY 1 cut(s) 941
AcuI CTGAAG 2 cut(s) 953, 1103
AfaI GTAC 5 cut(s) 98, 359, 394, 573, 839
AfiI CCNNNNNNNGG 3 cut(s) 286, 382, 556
AgsI TTSAA 5 cut(s) 196, 218, 655, 916, 947
AjnI CCWGG 2 cut(s) 279, 542
AleI CACNNNNGTG 1 cut(s) 707
AluBI AGCT 9 cut(s) 114, 136, 436, 482, 640, 668, 740, 876, 1039
AluI AGCT 9 cut(s) 114, 136, 436, 482, 640, 668, 740, 876, 1039
Alw21I GWGCWC 2 cut(s) 116, 784
Alw26I GTCTC 2 cut(s) 377, 416
Alw44I GTGCAC 1 cut(s) 780
Ama87I CYCGRG 1 cut(s) 401
Aor13HI TCCGGA 1 cut(s) 627
ApaLI GTGCAC 1 cut(s) 780
ApeKI GCWGC 5 cut(s) 449, 539, 737, 740, 749
ApoI RAATTY 1 cut(s) 941
AspLEI GCGC 1 cut(s) 180
AspS9I GGNCC 2 cut(s) 225, 547
AsuHPI GGTGA 1 cut(s) 466
AsuII TTCGAA 1 cut(s) 489
AvaI CYCGRG 1 cut(s) 401
AvaII GGWCC 2 cut(s) 225, 547
BaeGI GKGCMC 1 cut(s) 784
BanII GRGCYC 2 cut(s) 116, 850
BarI GAAGNNNNNNTAC 2 cut(s) 883, 915
BbsI GAAGAC 1 cut(s) 184
Bbv12I GWGCWC 2 cut(s) 116, 784
BbvI GCAGC 5 cut(s) 436, 551, 727, 736, 749
BccI CCATC 2 cut(s) 1067, 1096
BciT130I CCWGG 2 cut(s) 281, 544
BcoDI GTCTC 2 cut(s) 377, 416
BfaI CTAG 1 cut(s) 347
BfmI CTRYAG 1 cut(s) 16
BfuAI ACCTGC 2 cut(s) 767, 874
BisI GCNGC 6 cut(s) 450, 540, 738, 741, 750, 1136
BlsI GCNGC 6 cut(s) 451, 541, 739, 742, 751, 1137
BmcAI AGTACT 1 cut(s) 359
Bme1390I CCNGG 2 cut(s) 281, 544
Bme18I GGWCC 2 cut(s) 225, 547
BmeT110I CYCGRG 1 cut(s) 401
BmgT120I GGNCC 2 cut(s) 225, 547
BmiI GGNNCC 3 cut(s) 548, 549, 833
BmrFI CCNGG 2 cut(s) 281, 544
BmsI GCATC 4 cut(s) 28, 597, 611, 991
BpiI GAAGAC 1 cut(s) 184
BplI GAGNNNNNCTC 2 cut(s) 326, 358
BpmI CTGGAG 3 cut(s) 231, 359, 596
Bpu14I TTCGAA 1 cut(s) 489
BpuEI CTTGAG 2 cut(s) 290, 398
Bsa29I ATCGAT 1 cut(s) 605
BsaBI GATNNNNATC 1 cut(s) 1025
BsaJI CCNNGG 2 cut(s) 543, 771
BsaWI WCCGGW 1 cut(s) 627
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bsc4I CCNNNNNNNGG 3 cut(s) 286, 382, 556
Bse118I RCCGGY 1 cut(s) 834
Bse1I ACTGG 2 cut(s) 214, 789
Bse3DI GCAATG 2 cut(s) 512, 1130
Bse8I GATNNNNATC 1 cut(s) 1025
BseAI TCCGGA 1 cut(s) 627
BseBI CCWGG 2 cut(s) 281, 544
BseCI ATCGAT 1 cut(s) 605
BseDI CCNNGG 2 cut(s) 543, 771
BseGI GGATG 2 cut(s) 322, 1006
BseJI GATNNNNATC 1 cut(s) 1025
BseLI CCNNNNNNNGG 3 cut(s) 286, 382, 556
BseMI GCAATG 2 cut(s) 512, 1130
BseNI ACTGG 2 cut(s) 214, 789
BseRI GAGGAG 2 cut(s) 368, 575
BseSI GKGCMC 1 cut(s) 784
BseXI GCAGC 5 cut(s) 436, 551, 727, 736, 749
BsgI GTGCAG 2 cut(s) 441, 735
BshVI ATCGAT 1 cut(s) 605
BsiHKAI GWGCWC 2 cut(s) 116, 784
BsiHKCI CYCGRG 1 cut(s) 401
BsiSI CCGG 2 cut(s) 628, 835
BslFI GGGAC 1 cut(s) 560
BslI CCNNNNNNNGG 3 cut(s) 286, 382, 556
BsmAI GTCTC 2 cut(s) 377, 416
BsmFI GGGAC 1 cut(s) 560
BsmI GAATGC 2 cut(s) 311, 979
BsoBI CYCGRG 1 cut(s) 401
Bsp119I TTCGAA 1 cut(s) 489
Bsp1286I GDGCHC 3 cut(s) 116, 784, 850
Bsp13I TCCGGA 1 cut(s) 627
Bsp143I GATC 2 cut(s) 1011, 1026
BspACI CCGC 7 cut(s) 53, 59, 452, 460, 673, 755, 1135
BspDI ATCGAT 1 cut(s) 605
BspEI TCCGGA 1 cut(s) 627
BspLI GGNNCC 3 cut(s) 548, 549, 833
BspMAI CTGCAG 1 cut(s) 20
BspMI ACCTGC 2 cut(s) 767, 874
BspT104I TTCGAA 1 cut(s) 489
BsrDI GCAATG 2 cut(s) 512, 1130
BsrFI RCCGGY 1 cut(s) 834
BsrI ACTGG 2 cut(s) 214, 789
BssAI RCCGGY 1 cut(s) 834
BssECI CCNNGG 2 cut(s) 543, 771
BssMI GATC 2 cut(s) 1011, 1026
BssT1I CCWWGG 1 cut(s) 771
Bst2UI CCWGG 2 cut(s) 281, 544
Bst4CI ACNGT 1 cut(s) 371
Bst6I CTCTTC 3 cut(s) 626, 990, 1122
BstBI TTCGAA 1 cut(s) 489
BstC8I GCNNGC 1 cut(s) 420
BstF5I GGATG 2 cut(s) 322, 1006
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 2 cut(s) 1014, 1029
BstMAI GTCTC 2 cut(s) 377, 416
BstMBI GATC 2 cut(s) 1011, 1026
BstMWI GCNNNNNNNGC 5 cut(s) 67, 536, 746, 749, 983
BstNI CCWGG 2 cut(s) 281, 544
BstSCI CCNGG 2 cut(s) 279, 542
BstSFI CTRYAG 1 cut(s) 16
BstSLI GKGCMC 1 cut(s) 784
BstV1I GCAGC 5 cut(s) 436, 551, 727, 736, 749
BstV2I GAAGAC 1 cut(s) 184
Bsu15I ATCGAT 1 cut(s) 605
BsuTUI ATCGAT 1 cut(s) 605
BtsCI GGATG 2 cut(s) 322, 1006
BtsI GCAGTG 2 cut(s) 429, 962
BtsIMutI CAGTG 7 cut(s) 207, 255, 298, 429, 666, 782, 962
BveI ACCTGC 2 cut(s) 767, 874
Cac8I GCNNGC 1 cut(s) 420
CfoI GCGC 1 cut(s) 180
Cfr10I RCCGGY 1 cut(s) 834
Cfr13I GGNCC 2 cut(s) 225, 547
ClaI ATCGAT 1 cut(s) 605
Csp6I GTAC 5 cut(s) 97, 358, 393, 572, 838
CspCI CAANNNNNGTGG 2 cut(s) 196, 231
CviQI GTAC 5 cut(s) 97, 358, 393, 572, 838
DpnI GATC 2 cut(s) 1013, 1028
DpnII GATC 2 cut(s) 1011, 1026
Eam1104I CTCTTC 3 cut(s) 626, 990, 1122
EarI CTCTTC 3 cut(s) 626, 990, 1122
EciI GGCGGA 1 cut(s) 688
Ecl136II GAGCTC 1 cut(s) 114
Eco130I CCWWGG 1 cut(s) 771
Eco24I GRGCYC 2 cut(s) 116, 850
Eco47I GGWCC 2 cut(s) 225, 547
Eco53kI GAGCTC 1 cut(s) 114
Eco57I CTGAAG 2 cut(s) 953, 1103
Eco88I CYCGRG 1 cut(s) 401
EcoICRI GAGCTC 1 cut(s) 114
EcoO109I RGGNCCY 2 cut(s) 225, 547
EcoRII CCWGG 2 cut(s) 279, 542
EcoT14I CCWWGG 1 cut(s) 771
EcoT38I GRGCYC 2 cut(s) 116, 850
ErhI CCWWGG 1 cut(s) 771
FaiI YATR 8 cut(s) 120, 322, 591, 824, 896, 1052, 1141, 1143
FaqI GGGAC 1 cut(s) 560
FauI CCCGC 1 cut(s) 60
Fnu4HI GCNGC 6 cut(s) 450, 540, 738, 741, 750, 1136
FokI GGATG 2 cut(s) 329, 1013
FriOI GRGCYC 2 cut(s) 116, 850
Fsp4HI GCNGC 6 cut(s) 450, 540, 738, 741, 750, 1136
FspBI CTAG 1 cut(s) 347
GlaI GCGC 1 cut(s) 179
GluI GCNGC 6 cut(s) 450, 540, 738, 741, 750, 1136
GsuI CTGGAG 3 cut(s) 231, 359, 596
HapII CCGG 2 cut(s) 628, 835
HhaI GCGC 1 cut(s) 180
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HindIII AAGCTT 2 cut(s) 638, 666
HinfI GANTC 3 cut(s) 140, 189, 239
HpaII CCGG 2 cut(s) 628, 835
HphI GGTGA 1 cut(s) 466
Hpy166II GTNNAC 4 cut(s) 99, 149, 427, 782
Hpy188I TCNGA 5 cut(s) 94, 795, 852, 972, 1126
Hpy188III TCNNGA 8 cut(s) 302, 332, 376, 403, 613, 628, 952, 1015
Hpy8I GTNNAC 4 cut(s) 99, 149, 427, 782
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 3 cut(s) 307, 485, 490
HpyCH4III ACNGT 1 cut(s) 371
HpyCH4V TGCA 8 cut(s) 18, 309, 422, 517, 622, 752, 782, 883
HpyF10VI GCNNNNNNNGC 5 cut(s) 67, 536, 746, 749, 983
HspAI GCGC 1 cut(s) 178
KflI GGGWCCC 1 cut(s) 547
Kpn2I TCCGGA 1 cut(s) 627
Kzo9I GATC 2 cut(s) 1011, 1026
LmnI GCTCC 4 cut(s) 46, 615, 831, 1150
Lsp1109I GCAGC 5 cut(s) 436, 551, 727, 736, 749
LweI GCATC 4 cut(s) 28, 597, 611, 991
MaeI CTAG 1 cut(s) 347
MaeIII GTNAC 2 cut(s) 253, 763
MalI GATC 2 cut(s) 1013, 1028
MboI GATC 2 cut(s) 1011, 1026
MboII GAAGA 6 cut(s) 184, 482, 643, 1007, 1021, 1109
MhlI GDGCHC 3 cut(s) 116, 784, 850
MluCI AATT 3 cut(s) 441, 617, 941
MlyI GAGTC 2 cut(s) 198, 233
MmeI TCCRAC 3 cut(s) 196, 787, 868
MroI TCCGGA 1 cut(s) 627
MseI TTAA 2 cut(s) 534, 1070
MslI CAYNNNNRTG 2 cut(s) 707, 926
MspA1I CMGCKG 2 cut(s) 436, 740
MspI CCGG 2 cut(s) 628, 835
MspR9I CCNGG 2 cut(s) 281, 544
Mva1269I GAATGC 2 cut(s) 311, 979
MvaI CCWGG 2 cut(s) 281, 544
MwoI GCNNNNNNNGC 5 cut(s) 67, 536, 746, 749, 983
NdeII GATC 2 cut(s) 1011, 1026
NlaIV GGNNCC 3 cut(s) 548, 549, 833
NmeAIII GCCGAG 1 cut(s) 212
NmuCI GTSAC 1 cut(s) 253
NspV TTCGAA 1 cut(s) 489
OliI CACNNNNGTG 1 cut(s) 707
PaeR7I CTCGAG 1 cut(s) 401
PaqCI CACCTGC 1 cut(s) 767
PctI GAATGC 2 cut(s) 311, 979
PfeI GAWTC 1 cut(s) 140
PflMI CCANNNNNTGG 2 cut(s) 286, 382
PkrI GCNGC 6 cut(s) 451, 541, 739, 742, 751, 1137
PleI GAGTC 2 cut(s) 197, 233
PpsI GAGTC 2 cut(s) 197, 233
PpuMI RGGWCCY 2 cut(s) 225, 547
PsiI TTATAA 1 cut(s) 591
Psp124BI GAGCTC 1 cut(s) 116
Psp5II RGGWCCY 2 cut(s) 225, 547
Psp6I CCWGG 2 cut(s) 279, 542
PspGI CCWGG 2 cut(s) 279, 542
PspN4I GGNNCC 3 cut(s) 548, 549, 833
PspPI GGNCC 2 cut(s) 225, 547
PspPPI RGGWCCY 2 cut(s) 225, 547
PstI CTGCAG 1 cut(s) 20
PvuII CAGCTG 2 cut(s) 436, 740
RsaI GTAC 5 cut(s) 98, 359, 394, 573, 839
RsaNI GTAC 5 cut(s) 97, 358, 393, 572, 838
RseI CAYNNNNRTG 2 cut(s) 707, 926
SacI GAGCTC 1 cut(s) 116
SaqAI TTAA 2 cut(s) 534, 1070
SatI GCNGC 6 cut(s) 450, 540, 738, 741, 750, 1136
Sau3AI GATC 2 cut(s) 1011, 1026
Sau96I GGNCC 2 cut(s) 225, 547
ScaI AGTACT 1 cut(s) 359
SchI GAGTC 2 cut(s) 198, 233
ScrFI CCNGG 2 cut(s) 281, 544
SduI GDGCHC 3 cut(s) 116, 784, 850
SfaNI GCATC 4 cut(s) 28, 597, 611, 991
SfcI CTRYAG 1 cut(s) 16
Sfr274I CTCGAG 1 cut(s) 401
SfuI TTCGAA 1 cut(s) 489
SinI GGWCC 2 cut(s) 225, 547
SlaI CTCGAG 1 cut(s) 401
SmiMI CAYNNNNRTG 2 cut(s) 707, 926
SmlI CTYRAG 3 cut(s) 269, 401, 413
SmoI CTYRAG 3 cut(s) 269, 401, 413
Sse9I AATT 3 cut(s) 441, 617, 941
SsiI CCGC 7 cut(s) 53, 59, 452, 460, 673, 755, 1135
SspMI CTAG 1 cut(s) 347
SstI GAGCTC 1 cut(s) 116
StyD4I CCNGG 2 cut(s) 279, 542
StyI CCWWGG 1 cut(s) 771
TaaI ACNGT 1 cut(s) 371
TaqI TCGA 8 cut(s) 75, 138, 303, 402, 489, 605, 951, 1062
TasI AATT 3 cut(s) 441, 617, 941
TatI WGTACW 4 cut(s) 96, 357, 392, 571
TauI GCSGC 1 cut(s) 1138
TfiI GAWTC 1 cut(s) 140
Tru1I TTAA 2 cut(s) 534, 1070
Tru9I TTAA 2 cut(s) 534, 1070
TscAI CASTG 7 cut(s) 214, 262, 298, 429, 666, 789, 969
TseFI GTSAC 1 cut(s) 253
TseI GCWGC 5 cut(s) 449, 539, 737, 740, 749
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 1 cut(s) 290
TspGWI ACGGA 2 cut(s) 572, 923
TspRI CASTG 7 cut(s) 214, 262, 298, 429, 666, 789, 969
Van91I CCANNNNNTGG 2 cut(s) 286, 382
VneI GTGCAC 1 cut(s) 780
VpaK11BI GGWCC 2 cut(s) 225, 547
XapI RAATTY 1 cut(s) 941
XhoI CTCGAG 1 cut(s) 401
XspI CTAG 1 cut(s) 347
ZrmI AGTACT 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.