Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002983

Physical Location & Seq

Genomic Coordinates Reverse (-)

15041055 .. 15046686

Gene Structure 5632 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1172400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2238 bp

ATGATTGGTGGGCAATTCAAGCCTTTAAAGGGAGTGGCTGCCAATCACCTATATGGTTGTATCTCCCTCACTCATTCTCTCTCTCTCTCTCTCTCTCTCTCCCCTCCATATTTTCTCCCTCCTCAAATCCTCAGACTCACAGTCGTTATCTTTCCCCACAGAGAAAGAAAGCCCAAAGCTTCCATTCTTTTCATTCCAATTTTTTTCTTTATTTCTTCCTTCTTCTCAGAGCGCAAAGATATGGCTTCCGCTGTGTTCTGCAACTCCATGAACCCATCAGCGGCAGTTTTCTCAAGAACCCACCTTTCAGTTCCCGCCAACAAAGACTTCCCACTTGAATTTTCCCCATATTTTCATTACAACTGCCATTTGAGATGCAGAAGAAGAACTGGCAATAAGAAGTGTTTGACTGAGGTGAGAGCTGTGACGGAGGCGGCGCCACCCGCAGAGGCCCCCACCAGTGCACCCGTGCCGGCCCAGCAGAAGAAGAACCTGAGAATTCTGGTTGCCGGCGGTGGAATCGGAGGGTTGGTGTTTGCTCTCGCGGCGAAGAAGAAGGGGTTTGATGTGGTGGTGTTTGAGAAGGATTTGAGCGCTGTGAGAGGAGAGGGGCAGTACAGAGGTCCTATTCAGATACAGAGCAACGCGTTGGCGGCGTTGGAAGCCATTGATGTTGATGTTGCTGATGAGATTATGAAGGTTGGATGTGTTACTGGGGACAGGATCAATGGCCTCGTTGATGGGGTTTCTGGTACTTGGTATGTCAAGTTTGACACATTCACTCCTGCAGTGGAACGAGGGCTTCCAGTTACAAGAGTTATAAGCCGAATGACCTTGCAACAAATCCTTGCTCGTGCTGTTGGGGACAATATTATTATAAATGGTAGTAATGTTGTTAACTTTGAGGATGTTGGAGATAAGGTTAATGTGATACTTGAGAATGGAGAACGTTTTGAAGGTGATATTCTGGTTGGAGCCGATGGTATATGGTCAAAGGTGAGGCAGAACTTGTTTGGATTATCGGACGCTGTCTACTCTGGTTATACTTGTTACACTGGTATCGCAGATTTTGTGCCTGCTGACATTAATTCTGTAGGGTACCGAGTATTTTTGGGGCACAAGCAATACTTCGTTTCTTCAGATGTAGGTGCAGGAAAGATGCAGTGGTATGCGTTTCACAAGGAAGCACCTGGTGGTGTTGATAGCCCCAATGGTAAAAAGGAAAGGCTGCTCAAAATATTTGAGGGCTGGTGTGATAATGTGATAGATTTGTTACTTGCCACAGAGGAAGATGCAATTCTACGACGTGACATATATGATAGGACGCCCATCCTAACTTGGGGAAAGGGTCATGTGACCTTGCTTGGCGATTCTGTTCATGCTATGCAGCCAAATATGGGTCAAGGGGGATGCATGGCCATTGAGGATGGTTATCAACTTGCCATGGAGCTTGATAAAGCATGGCAGAAAAGTAGCGAATCGGGAACTCCTATTAACATTAATTCTTCTTTAAGAAGCTATGAGAATTCTAGAAGACTACGGGTCGCGATTATTCATGGAATGGCCAGAATGGCTGCTCTAATGGCTTCTACTTACAAGGCGTATCTGGGTGTGGGACTTGGTCCTTTTTCGTTTTTGACAAAGTTTCGGATACCACATCCGGGAAGAGTTGGTGGGAGAGTTTTTATTGATAAGGCAATGCCCCTGATGCTAAGTTGGGTCTTAGGTGGTAACAGCTCAAAACTTGAAGGAAGGTCCCCCAGATGCAGGCTCTCAGATAAAGCAAGTGACCAGTTGCGAACATGGTTTGAAGACGATGATGCACTGGAGCGTGCTATTGACGGAGAGTGGTATCTTATACCCTGTGGGCAAGACAATGATGCTTCACAACTTATTTGTTTGAATCGAGATGAGAAAACATCCTGCATAATTGGGAGTATACCGCATGGGGATGCTTCTGGAACATCAATAACAATACGCTCGCCCCAGGTCTCAGAGATGCATGCTCGTATCAGCTACAAGGATGGTGCCTTCTACGTGACTGATTTGCAAAGCGAACATGGTACCTGGATTGCCGATATTGAGGAGAAGCGATACCGAGTACCCCCAAATTTCCCTGCTCGTATCCATCCATCCGATGCTATTGAGATTGGTTCCCAGAAGGTAGCATTCAGGGTTAAAGTTATGAAGTCTTCTCCGAAGGGTGCCGAGAAGGACGGAGTTCTTCAGGCCGCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009266 GO:0009314 GO:0009408 GO:0009414 GO:0009415 GO:0009416 GO:0009507 GO:0009526 GO:0009536 GO:0009540 GO:0009628 GO:0009639 GO:0009687 GO:0009688 GO:0009743 GO:0009756 GO:0009941 GO:0009987 GO:0010033 GO:0010035 GO:0010114 GO:0010182 GO:0016053 GO:0016106 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016122 GO:0016123 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0019752 GO:0023052 GO:0030104 GO:0031967 GO:0031975 GO:0032501 GO:0032787 GO:0042221 GO:0042440 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0046148 GO:0046165 GO:0046394 GO:0048871 GO:0048878 GO:0050789 GO:0050794 GO:0050878 GO:0050891 GO:0050896 GO:0051716 GO:0052662 GO:0055114 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071704 GO:0072330 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

ko00906 ko01100 ko01110 map00906 map01100 map01110

Pfam Domains

Protein Families

FAD_binding_3 FHA NAD_binding_8

Protein Analysis

746

Amino Acids

81.84

Weight (kDa)

8.51

Isoelectric Point (pI)

40.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_3	PF01494	446 - 489	4.4e-06	FAD binding domain
FHA	PF00498	653 - 718	4.5e-11	FHA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002983)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67030 AT5G67030
fragaria_vesca	FvH4_1g16080 FvH4_1g16080 FvH4_1g16080 FvH4_1g16080 FvH4_1g16080
malus_domestica	MD02G1172400.v1.1 MD15G1284500.v1.1
prunus_persica	Prupe.7G133100_v2.0.a1 Prupe.7G133100_v2.0.a1
pyrus_communis	pycom02g13890 pycom15g24720
rosa_chinensis	RchiOBHm_Chr2g0105701 RchiOBHm_Chr2g0138261 RchiOBHm_Chr5g0062971 RchiOBHm_Chr5g0062981
rosa_laevigata	RLG00000006425 RLG00000017450
rosa_multiflora	Rmu_sc0001051.1_g000057
rosa_roxburghii	Rroxscaffold_2G00137970 Rroxscaffold_2G00137980
rosa_rugosa	Rorug02G0129300 Rorug02G0129400 Rorug02G0129500 Rorug02G0129600
rosa_samantha	Rh2AG181000 Rh2BG190100 Rh2CG185200 Rh2DG187000 Rh4DG142500 Rh7AG320600 Rh7AG325500 Rh7BG187500 Rh7CG337400 Rh7CG342700
rosa_wichuraiana	Rw2G014150 Rw2G041180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	821, 878
Acc65I	GGTACC	2 cut(s)	1098, 2063
AccB1I	GGYRCC	5 cut(s)	436, 1098, 2027, 2063, 2204
AccI	GTMKAC	2 cut(s)	1032, 1939
AccII	CGCG	4 cut(s)	545, 647, 1547, 2234
AclI	AACGTT	1 cut(s)	949
AclWI	GGATC	1 cut(s)	731
AcoI	YGGCCR	2 cut(s)	1416, 1563
AcsI	RAATTY	4 cut(s)	338, 498, 1525, 2110
AcuI	CTGAAG	2 cut(s)	1122, 2210
AcyI	GRCGYC	2 cut(s)	437, 1325
AdeI	CACNNNGTG	1 cut(s)	1193
AfaI	GTAC	5 cut(s)	617, 754, 1100, 2065, 2103
AfeI	AGCGCT	1 cut(s)	595
AfiI	CCNNNNNNNGG	6 cut(s)	29, 280, 1339, 1397, 1661, 1767
AflIII	ACRYGT	1 cut(s)	645
AgsI	TTSAA	6 cut(s)	19, 338, 956, 1748, 1811, 1903
AhdI	GACNNNNNGTC	2 cut(s)	140, 1541
AjiI	CACGTC	1 cut(s)	1307
AjnI	CCWGG	3 cut(s)	1189, 1986, 2066
AjuI	GAANNNNNNNTTGG	2 cut(s)	311, 343
AluBI	AGCT	6 cut(s)	179, 422, 1450, 1518, 1737, 2016
AluI	AGCT	6 cut(s)	179, 422, 1450, 1518, 1737, 2016
Alw21I	GWGCWC	1 cut(s)	466
Alw26I	GTCTC	1 cut(s)	1996
Alw44I	GTGCAC	1 cut(s)	462
AlwI	GGATC	1 cut(s)	731
Aor51HI	AGCGCT	1 cut(s)	595
AoxI	GGCC	6 cut(s)	450, 474, 730, 1416, 1563, 2229
ApaLI	GTGCAC	1 cut(s)	462
ApeKI	GCWGC	4 cut(s)	38, 1228, 1387, 1574
ApoI	RAATTY	4 cut(s)	338, 498, 1525, 2110
AseI	ATTAAT	2 cut(s)	1086, 1500
Asp718I	GGTACC	2 cut(s)	1098, 2063
AspLEI	GCGC	3 cut(s)	234, 439, 596
AspS9I	GGNCC	5 cut(s)	451, 475, 623, 1622, 1755
AsuC2I	CCSGG	1 cut(s)	1662
AsuHPI	GGTGA	4 cut(s)	38, 427, 971, 1009
AvaII	GGWCC	3 cut(s)	623, 1622, 1755
BaeGI	GKGCMC	2 cut(s)	466, 1119
BaeI	ACNNNNGTAYC	2 cut(s)	1042, 1075
BalI	TGGCCA	2 cut(s)	1418, 1565
BanI	GGYRCC	5 cut(s)	436, 1098, 2027, 2063, 2204
BauI	CACGAG	1 cut(s)	852
BbsI	GAAGAC	3 cut(s)	1540, 1818, 2184
Bbv12I	GWGCWC	1 cut(s)	466
BbvI	GCAGC	4 cut(s)	25, 1215, 1399, 1561
BccI	CCATC	8 cut(s)	283, 734, 974, 1337, 1421, 2018, 2136, 2140
BciT130I	CCWGG	3 cut(s)	1191, 1988, 2068
BciVI	GTATCC	2 cut(s)	1644, 2135
BcnI	CCSGG	1 cut(s)	1662
BcoDI	GTCTC	1 cut(s)	1996
BfaI	CTAG	1 cut(s)	1530
BfmI	CTRYAG	2 cut(s)	786, 1092
BfoI	RGCGCY	2 cut(s)	440, 597
BfuI	GTATCC	2 cut(s)	1644, 2135
BglI	GCCNNNNNGGC	1 cut(s)	1571
BisI	GCNGC	9 cut(s)	39, 282, 435, 546, 654, 1229, 1388, 1575, 2232
BlsI	GCNGC	9 cut(s)	40, 283, 436, 547, 655, 1230, 1389, 1576, 2233
Bme1390I	CCNGG	4 cut(s)	1191, 1662, 1988, 2068
Bme18I	GGWCC	3 cut(s)	623, 1622, 1755
BmeRI	GACNNNNNGTC	2 cut(s)	140, 1541
BmgBI	CACGTC	1 cut(s)	1307
BmgT120I	GGNCC	5 cut(s)	451, 475, 623, 1622, 1755
BmiI	GGNNCC	9 cut(s)	438, 453, 976, 1100, 1757, 2029, 2065, 2155, 2206
BmrFI	CCNGG	4 cut(s)	1191, 1662, 1988, 2068
BmrI	ACTGGG	1 cut(s)	723
BmuI	ACTGGG	1 cut(s)	723
BpiI	GAAGAC	3 cut(s)	1540, 1818, 2184
BpmI	CTGGAG	1 cut(s)	1847
BpuEI	CTTGAG	2 cut(s)	277, 956
BpuMI	CCSGG	1 cut(s)	1662
BsaAI	YACGTR	1 cut(s)	2038
BsaBI	GATNNNNATC	1 cut(s)	1431
BsaHI	GRCGYC	2 cut(s)	437, 1325
BsaI	GGTCTC	1 cut(s)	1996
BsaJI	CCNNGG	2 cut(s)	1443, 1986
BsaXI	ACNNNNNCTCC	6 cut(s)	516, 546, 766, 796, 906, 936
Bsc4I	CCNNNNNNNGG	6 cut(s)	29, 280, 1339, 1397, 1661, 1767
Bse118I	RCCGGY	2 cut(s)	472, 509
Bse1I	ACTGG	7 cut(s)	394, 459, 718, 806, 1060, 1792, 1830
Bse3DI	GCAATG	1 cut(s)	1704
Bse8I	GATNNNNATC	1 cut(s)	1431
BseBI	CCWGG	3 cut(s)	1191, 1988, 2068
BseDI	CCNNGG	2 cut(s)	1443, 1986
BseJI	GATNNNNATC	1 cut(s)	1431
BseLI	CCNNNNNNNGG	6 cut(s)	29, 280, 1339, 1397, 1661, 1767
BseMI	GCAATG	1 cut(s)	1704
BseMII	CTCAG	6 cut(s)	145, 240, 402, 485, 1788, 2007
BseNI	ACTGG	7 cut(s)	394, 459, 718, 806, 1060, 1792, 1830
BseRI	GAGGAG	3 cut(s)	111, 618, 2099
BseSI	GKGCMC	2 cut(s)	466, 1119
BseXI	GCAGC	4 cut(s)	25, 1215, 1399, 1561
BseYI	CCCAGC	1 cut(s)	477
BsgI	GTGCAG	1 cut(s)	1170
Bsh1236I	CGCG	4 cut(s)	545, 647, 1547, 2234
BshFI	GGCC	6 cut(s)	452, 476, 732, 1418, 1565, 2231
BshNI	GGYRCC	5 cut(s)	436, 1098, 2027, 2063, 2204
BsiHKAI	GWGCWC	1 cut(s)	466
BsiSI	CCGG	3 cut(s)	473, 510, 1661
BslFI	GGGAC	4 cut(s)	731, 878, 1629, 1741
BslI	CCNNNNNNNGG	6 cut(s)	29, 280, 1339, 1397, 1661, 1767
BsmAI	GTCTC	1 cut(s)	1996
BsmFI	GGGAC	4 cut(s)	731, 878, 1629, 1741
BsmI	GAATGC	1 cut(s)	2168
BsnI	GGCC	6 cut(s)	452, 476, 732, 1418, 1565, 2231
Bso31I	GGTCTC	1 cut(s)	1996
Bsp1286I	GDGCHC	2 cut(s)	466, 1119
Bsp143I	GATC	1 cut(s)	723
Bsp19I	CCATGG	1 cut(s)	1443
Bsp68I	TCGCGA	1 cut(s)	1547
BspANI	GGCC	6 cut(s)	452, 476, 732, 1418, 1565, 2231
BspCNI	CTCAG	6 cut(s)	144, 239, 403, 486, 1787, 2006
BspFNI	CGCG	4 cut(s)	545, 647, 1547, 2234
BspLI	GGNNCC	9 cut(s)	438, 453, 976, 1100, 1757, 2029, 2065, 2155, 2206
BspMAI	CTGCAG	1 cut(s)	790
BspPI	GGATC	1 cut(s)	731
BspT107I	GGYRCC	5 cut(s)	436, 1098, 2027, 2063, 2204
BspTNI	GGTCTC	1 cut(s)	1996
BsrDI	GCAATG	1 cut(s)	1704
BsrFI	RCCGGY	2 cut(s)	472, 509
BsrI	ACTGG	7 cut(s)	394, 459, 718, 806, 1060, 1792, 1830
BssAI	RCCGGY	2 cut(s)	472, 509
BssECI	CCNNGG	2 cut(s)	1443, 1986
BssMI	GATC	1 cut(s)	723
BssNAI	GTATAC	1 cut(s)	1940
BssNI	GRCGYC	2 cut(s)	437, 1325
BssSI	CACGAG	1 cut(s)	852
BssT1I	CCWWGG	1 cut(s)	1443
Bst1107I	GTATAC	1 cut(s)	1940
Bst2BI	CACGAG	1 cut(s)	852
Bst2UI	CCWGG	3 cut(s)	1191, 1988, 2068
Bst4CI	ACNGT	1 cut(s)	142
Bst6I	CTCTTC	1 cut(s)	1660
BstACI	GRCGYC	2 cut(s)	437, 1325
BstBAI	YACGTR	1 cut(s)	2038
BstC8I	GCNNGC	7 cut(s)	474, 511, 1077, 1769, 1833, 1982, 2004
BstDEI	CTNAG	8 cut(s)	131, 226, 411, 494, 1712, 1723, 1774, 1993
BstDSI	CCRYGG	1 cut(s)	1443
BstFNI	CGCG	4 cut(s)	545, 647, 1547, 2234
BstH2I	RGCGCY	2 cut(s)	440, 597
BstHHI	GCGC	3 cut(s)	234, 439, 596
BstKTI	GATC	1 cut(s)	726
BstMAI	GTCTC	1 cut(s)	1996
BstMBI	GATC	1 cut(s)	723
BstMWI	GCNNNNNNNGC	9 cut(s)	19, 443, 478, 545, 653, 662, 1571, 1583, 1708
BstNI	CCWGG	3 cut(s)	1191, 1988, 2068
BstNSI	RCATGY	1 cut(s)	2006
BstSCI	CCNGG	4 cut(s)	1189, 1660, 1986, 2066
BstSFI	CTRYAG	2 cut(s)	786, 1092
BstSLI	GKGCMC	2 cut(s)	466, 1119
BstUI	CGCG	4 cut(s)	545, 647, 1547, 2234
BstV1I	GCAGC	4 cut(s)	25, 1215, 1399, 1561
BstV2I	GAAGAC	3 cut(s)	1540, 1818, 2184
BstZ17I	GTATAC	1 cut(s)	1940
BsuI	GTATCC	2 cut(s)	1644, 2135
BsuRI	GGCC	6 cut(s)	452, 476, 732, 1418, 1565, 2231
BtgI	CCRYGG	1 cut(s)	1443
BtrI	CACGTC	1 cut(s)	1307
BtsI	GCAGTG	2 cut(s)	795, 1169
BtsIMutI	CAGTG	5 cut(s)	466, 795, 1053, 1169, 1823
BtuMI	TCGCGA	1 cut(s)	1547
Cac8I	GCNNGC	7 cut(s)	474, 511, 1077, 1769, 1833, 1982, 2004
CfoI	GCGC	3 cut(s)	234, 439, 596
Cfr10I	RCCGGY	2 cut(s)	472, 509
Cfr13I	GGNCC	5 cut(s)	451, 475, 623, 1622, 1755
CseI	GACGC	2 cut(s)	1034, 1333
CsiI	ACCWGGT	1 cut(s)	1189
Csp6I	GTAC	5 cut(s)	616, 753, 1099, 2064, 2102
CviQI	GTAC	5 cut(s)	616, 753, 1099, 2064, 2102
DdeI	CTNAG	8 cut(s)	131, 226, 411, 494, 1712, 1723, 1774, 1993
DinI	GGCGCC	1 cut(s)	438
DpnI	GATC	1 cut(s)	725
DpnII	GATC	1 cut(s)	723
DraI	TTTAAA	1 cut(s)	27
DraIII	CACNNNGTG	1 cut(s)	1193
DriI	GACNNNNNGTC	2 cut(s)	140, 1541
EaeI	YGGCCR	2 cut(s)	1416, 1563
Eam1104I	CTCTTC	1 cut(s)	1660
Eam1105I	GACNNNNNGTC	2 cut(s)	140, 1541
EarI	CTCTTC	1 cut(s)	1660
Eco130I	CCWWGG	1 cut(s)	1443
Eco31I	GGTCTC	1 cut(s)	1996
Eco47I	GGWCC	3 cut(s)	623, 1622, 1755
Eco47III	AGCGCT	1 cut(s)	595
Eco57I	CTGAAG	2 cut(s)	1122, 2210
EcoO109I	RGGNCCY	3 cut(s)	451, 623, 1755
EcoRI	GAATTC	2 cut(s)	498, 1525
EcoRII	CCWGG	3 cut(s)	1189, 1986, 2066
EcoT14I	CCWWGG	1 cut(s)	1443
EcoT22I	ATGCAT	2 cut(s)	1415, 2004
EgeI	GGCGCC	1 cut(s)	438
EheI	GGCGCC	1 cut(s)	438
ErhI	CCWWGG	1 cut(s)	1443
FalI	AAGNNNNNCTT	2 cut(s)	1112, 1144
FaqI	GGGAC	4 cut(s)	731, 878, 1629, 1741
FauI	CCCGC	2 cut(s)	322, 451
FblI	GTMKAC	2 cut(s)	1032, 1939
Fnu4HI	GCNGC	9 cut(s)	39, 282, 435, 546, 654, 1229, 1388, 1575, 2232
Fsp4HI	GCNGC	9 cut(s)	39, 282, 435, 546, 654, 1229, 1388, 1575, 2232
FspBI	CTAG	1 cut(s)	1530
GlaI	GCGC	3 cut(s)	233, 438, 595
GluI	GCNGC	9 cut(s)	39, 282, 435, 546, 654, 1229, 1388, 1575, 2232
GsaI	CCCAGC	1 cut(s)	481
GsuI	CTGGAG	1 cut(s)	1847
HaeII	RGCGCY	2 cut(s)	440, 597
HaeIII	GGCC	6 cut(s)	452, 476, 732, 1418, 1565, 2231
HapII	CCGG	3 cut(s)	473, 510, 1661
HgaI	GACGC	2 cut(s)	1034, 1333
HhaI	GCGC	3 cut(s)	234, 439, 596
Hin1I	GRCGYC	2 cut(s)	437, 1325
Hin6I	GCGC	3 cut(s)	232, 437, 594
HinP1I	GCGC	3 cut(s)	232, 437, 594
HincII	GTYRAC	1 cut(s)	898
HindII	GTYRAC	1 cut(s)	898
HindIII	AAGCTT	1 cut(s)	177
HinfI	GANTC	5 cut(s)	135, 519, 1370, 1478, 1903
HpaI	GTTAAC	1 cut(s)	898
HpaII	CCGG	3 cut(s)	473, 510, 1661
HphI	GGTGA	4 cut(s)	38, 427, 971, 1009
Hpy166II	GTNNAC	4 cut(s)	464, 898, 1033, 1940
Hpy188III	TCNNGA	6 cut(s)	294, 1482, 1530, 1546, 1907, 1959
Hpy8I	GTNNAC	4 cut(s)	464, 898, 1033, 1940
Hpy99I	CGWCG	1 cut(s)	1308
HpyCH4III	ACNGT	1 cut(s)	142
HpyCH4IV	ACGT	3 cut(s)	949, 1306, 2037
HpyF10VI	GCNNNNNNNGC	9 cut(s)	19, 443, 478, 545, 653, 662, 1571, 1583, 1708
HpyF3I	CTNAG	8 cut(s)	131, 226, 411, 494, 1712, 1723, 1774, 1993
HpySE526I	ACGT	3 cut(s)	949, 1306, 2037
Hsp92I	GRCGYC	2 cut(s)	437, 1325
HspAI	GCGC	3 cut(s)	232, 437, 594
KasI	GGCGCC	1 cut(s)	436
KpnI	GGTACC	2 cut(s)	1102, 2067
KroI	GCCGGC	2 cut(s)	472, 509
KroNI	GCCGGC	2 cut(s)	474, 511
KspAI	GTTAAC	1 cut(s)	898
Kzo9I	GATC	1 cut(s)	723
LmnI	GCTCC	3 cut(s)	974, 1447, 1828
Lsp1109I	GCAGC	4 cut(s)	25, 1215, 1399, 1561
MabI	ACCWGGT	1 cut(s)	1189
MaeI	CTAG	1 cut(s)	1530
MaeII	ACGT	3 cut(s)	949, 1306, 2037
MalI	GATC	1 cut(s)	725
MboI	GATC	1 cut(s)	723
MhlI	GDGCHC	2 cut(s)	466, 1119
MlsI	TGGCCA	2 cut(s)	1418, 1565
MluI	ACGCGT	1 cut(s)	645
MluNI	TGGCCA	2 cut(s)	1418, 1565
Mly113I	GGCGCC	1 cut(s)	437
MlyI	GAGTC	1 cut(s)	129
MmeI	TCCRAC	4 cut(s)	639, 682, 892, 952
Mox20I	TGGCCA	2 cut(s)	1418, 1565
Mph1103I	ATGCAT	2 cut(s)	1415, 2004
MroNI	GCCGGC	2 cut(s)	472, 509
MscI	TGGCCA	2 cut(s)	1418, 1565
MseI	TTAA	8 cut(s)	26, 897, 924, 1086, 1494, 1500, 1511, 2178
MslI	CAYNNNNRTG	2 cut(s)	51, 1950
Msp20I	TGGCCA	2 cut(s)	1418, 1565
MspA1I	CMGCKG	2 cut(s)	251, 281
MspI	CCGG	3 cut(s)	473, 510, 1661
MspR9I	CCNGG	4 cut(s)	1191, 1662, 1988, 2068
Mva1269I	GAATGC	1 cut(s)	2168
MvaI	CCWGG	3 cut(s)	1191, 1988, 2068
MvnI	CGCG	4 cut(s)	545, 647, 1547, 2234
MwoI	GCNNNNNNNGC	9 cut(s)	19, 443, 478, 545, 653, 662, 1571, 1583, 1708
NaeI	GCCGGC	2 cut(s)	474, 511
NarI	GGCGCC	1 cut(s)	437
NciI	CCSGG	1 cut(s)	1662
NcoI	CCATGG	1 cut(s)	1443
NdeII	GATC	1 cut(s)	723
NgoMIV	GCCGGC	2 cut(s)	472, 509
NlaIV	GGNNCC	9 cut(s)	438, 453, 976, 1100, 1757, 2029, 2065, 2155, 2206
NmeAIII	GCCGAG	1 cut(s)	2233
NmuCI	GTSAC	5 cut(s)	424, 1307, 1354, 1787, 2038
NruI	TCGCGA	1 cut(s)	1547
NsiI	ATGCAT	2 cut(s)	1415, 2004
NspI	RCATGY	1 cut(s)	2006
PaeI	GCATGC	1 cut(s)	2006
PctI	GAATGC	1 cut(s)	2168
PdiI	GCCGGC	2 cut(s)	474, 511
PfeI	GAWTC	4 cut(s)	519, 1370, 1478, 1903
PflFI	GACNNNGTC	2 cut(s)	1028, 1620
PfoI	TCCNGGA	1 cut(s)	1660
PkrI	GCNGC	9 cut(s)	40, 283, 436, 547, 655, 1230, 1389, 1576, 2233
PleI	GAGTC	1 cut(s)	129
PluTI	GGCGCC	1 cut(s)	440
PpsI	GAGTC	1 cut(s)	129
Ppu21I	YACGTR	1 cut(s)	2038
PpuMI	RGGWCCY	2 cut(s)	623, 1755
PshBI	ATTAAT	2 cut(s)	1086, 1500
PsiI	TTATAA	2 cut(s)	821, 878
Psp1406I	AACGTT	1 cut(s)	949
Psp5II	RGGWCCY	2 cut(s)	623, 1755
Psp6I	CCWGG	3 cut(s)	1189, 1986, 2066
PspFI	CCCAGC	1 cut(s)	477
PspGI	CCWGG	3 cut(s)	1189, 1986, 2066
PspN4I	GGNNCC	9 cut(s)	438, 453, 976, 1100, 1757, 2029, 2065, 2155, 2206
PspPI	GGNCC	5 cut(s)	451, 475, 623, 1622, 1755
PspPPI	RGGWCCY	2 cut(s)	623, 1755
PstI	CTGCAG	1 cut(s)	790
PsyI	GACNNNGTC	2 cut(s)	1028, 1620
RruI	TCGCGA	1 cut(s)	1547
RsaI	GTAC	5 cut(s)	617, 754, 1100, 2065, 2103
RsaNI	GTAC	5 cut(s)	616, 753, 1099, 2064, 2102
RseI	CAYNNNNRTG	2 cut(s)	51, 1950
SaqAI	TTAA	8 cut(s)	26, 897, 924, 1086, 1494, 1500, 1511, 2178
SatI	GCNGC	9 cut(s)	39, 282, 435, 546, 654, 1229, 1388, 1575, 2232
Sau3AI	GATC	1 cut(s)	723
Sau96I	GGNCC	5 cut(s)	451, 475, 623, 1622, 1755
SchI	GAGTC	1 cut(s)	129
ScrFI	CCNGG	4 cut(s)	1191, 1662, 1988, 2068
SduI	GDGCHC	2 cut(s)	466, 1119
SexAI	ACCWGGT	1 cut(s)	1189
SfcI	CTRYAG	2 cut(s)	786, 1092
SfoI	GGCGCC	1 cut(s)	438
SinI	GGWCC	3 cut(s)	623, 1622, 1755
SmiMI	CAYNNNNRTG	2 cut(s)	51, 1950
SmlI	CTYRAG	2 cut(s)	292, 935
SmoI	CTYRAG	2 cut(s)	292, 935
SphI	GCATGC	1 cut(s)	2006
SspDI	GGCGCC	1 cut(s)	436
SspI	AATATT	2 cut(s)	871, 1239
SspMI	CTAG	1 cut(s)	1530
StyD4I	CCNGG	4 cut(s)	1189, 1660, 1986, 2066
StyI	CCWWGG	1 cut(s)	1443
TaaI	ACNGT	1 cut(s)	142
TaiI	ACGT	3 cut(s)	952, 1309, 2040
TaqI	TCGA	1 cut(s)	1906
TatI	WGTACW	1 cut(s)	615
TauI	GCSGC	5 cut(s)	284, 437, 548, 656, 2234
TfiI	GAWTC	4 cut(s)	519, 1370, 1478, 1903
Tru1I	TTAA	8 cut(s)	26, 897, 924, 1086, 1494, 1500, 1511, 2178
Tru9I	TTAA	8 cut(s)	26, 897, 924, 1086, 1494, 1500, 1511, 2178
TscAI	CASTG	5 cut(s)	466, 795, 1060, 1169, 1830
TseFI	GTSAC	5 cut(s)	424, 1307, 1354, 1787, 2038
TseI	GCWGC	4 cut(s)	38, 1228, 1387, 1574
Tsp45I	GTSAC	5 cut(s)	424, 1307, 1354, 1787, 2038
TspDTI	ATGAA	7 cut(s)	181, 284, 344, 710, 1367, 1544, 2201
TspGWI	ACGGA	3 cut(s)	443, 1857, 2232
TspRI	CASTG	5 cut(s)	466, 795, 1060, 1169, 1830
Tth111I	GACNNNGTC	2 cut(s)	1028, 1620
VneI	GTGCAC	1 cut(s)	462
VpaK11BI	GGWCC	3 cut(s)	623, 1622, 1755
VspI	ATTAAT	2 cut(s)	1086, 1500
XapI	RAATTY	4 cut(s)	338, 498, 1525, 2110
XbaI	TCTAGA	1 cut(s)	1529
XceI	RCATGY	1 cut(s)	2006
XmiI	GTMKAC	2 cut(s)	1032, 1939
XspI	CTAG	1 cut(s)	1530
Zsp2I	ATGCAT	2 cut(s)	1415, 2004

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.