Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003316

Physical Location & Seq

Genomic Coordinates Forward (+)

19837720 .. 19840075

Gene Structure 2356 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1200900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 951 bp

ATGCTGGTCTGGTCTGTCTCTCTGGCTTTTCAATTCCCAGAAACTCTCCCTCAAGAAAGAAAAATCCCAGAATTCCATTCCGGAAAAAACCACGGGAAAATGGAATCGAAAACCCAGAAACCGCAAGAATCCTCGAAGAAAGATTGGTCCTTGCGAGACTTCGAGATCGGAAAACCCCTCGGAAAGGGGAAATTCGGCCGAGTCTACGTCGCCCGAGAAGCCAAGAGCAAGTACGTAGTGGCATTGAAGGTAATATTCAAGGAGCAGATCGAAAAGTACAAGATTCAGCATCAGCTGAGGAGGGAGATGGAGATCCAGACCAGCCTCCGCCACCCCAACATCCTCCGCCTCTACGGCTGGTTCCACGATGACGACCGCATCTTCTTGATCCTCGAGTACGCTCACGGCGGAGAGCTCTACGGACTTCTCAGGAAAACTACCTACCTTTCGGAGAAACAGGCCGCCACTTATATTTTGAGCTTGACTGAAGCATTGGCGTATTGTCACGAAAAGCATGTGATTCATAGGGACATCAAGCCTGAAAATTTGCTATTGGATCATGAGGGTCGATTGAAAATTGCAGACTTTGGATGGTCTGTACAGTCAAGAAGCAAGAGGCATACCATGTGTGGAACTCTAGATTATTTAGCACCAGAAATGGTGGAGAACAAAGCTCATGATTATGCTGTTGATAACTGGACTTTAGGCGTTCTTTGCTATGAGTTCCTCTTTGGTATTCCTCCCTTTGAGGCCGAAAGTCAAAAGGATACATTCAAAAGGATAATGAAGGTTGACCTAAGCTTCCCAGCTGAACCTCAAGTTTCCGCGGAGGCTAAACATCTCATTACCAGGCTTCTCGTCAAGGACTCCTCAAAGAGGCTGTCTCTTCAAAAGATCATGGAACACCCTTGGATAATCAAGAACACAGATCCCTCCGGCATTTGCAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000228 GO:0000278 GO:0000775 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0005875 GO:0005876 GO:0006325 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0008150 GO:0008152 GO:0009987 GO:0010564 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016569 GO:0016570 GO:0016572 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022402 GO:0031974 GO:0031981 GO:0032133 GO:0032465 GO:0032991 GO:0035173 GO:0035174 GO:0035175 GO:0035404 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043987 GO:0043988 GO:0044022 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0048471 GO:0050789 GO:0050794 GO:0051233 GO:0051276 GO:0051302 GO:0051726 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0098687 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140096 GO:1901564 GO:1902850 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

317

Amino Acids

36.82

Weight (kDa)

9.09

Isoelectric Point (pI)

38.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	54 - 305	3.4e-78	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	55 - 301	3.6e-43	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003316)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45490 AT2G45490
fragaria_vesca	FvH4_4g03900 FvH4_4g03900 FvH4_4g03900 FvH4_4g03900 FvH4_4g03900 FvH4_7g12170 FvH4_7g12170 FvH4_7g12170 FvH4_7g12170
malus_domestica	MD02G1200900.v1.1
prunus_persica	Prupe.2G148200_v2.0.a1 Prupe.2G148200_v2.0.a1
pyrus_communis	pycom02g16420
rosa_chinensis	RchiOBHm_Chr1g0350921 RchiOBHm_Chr4g0393931
rosa_laevigata	RLG00000028441
rosa_multiflora	Rmu_sc0002665.1_g000020 Rmu_sc0014584.1_g000001
rosa_roxburghii	Rroxscaffold_4G00304300 Rroxscaffold_5G00339010
rosa_rugosa	Rorug01G0215300 Rorug03G0370400 Rorug03G0370500
rosa_samantha	Rh1AG230000 Rh1BG199900 Rh1CG214400 Rh1DG227700 Rh4AG049600 Rh4BG046000 Rh4CG053100 Rh4DG046600
rosa_wichuraiana	Rw1G019910 Rw1G019940 Rw4G003930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	204
AccII	CGCG	1 cut(s)	827
AccIII	TCCGGA	1 cut(s)	80
AciI	CCGC	8 cut(s)	122, 328, 346, 376, 408, 462, 825, 827
AclWI	GGATC	4 cut(s)	307, 382, 564, 923
AcoI	YGGCCR	1 cut(s)	196
AcsI	RAATTY	3 cut(s)	71, 191, 544
AcuI	CTGAAG	1 cut(s)	507
AfaI	GTAC	4 cut(s)	233, 278, 398, 600
AfiI	CCNNNNNNNGG	2 cut(s)	184, 876
AgsI	TTSAA	6 cut(s)	32, 247, 259, 574, 775, 890
AjnI	CCWGG	1 cut(s)	848
AluBI	AGCT	6 cut(s)	295, 415, 480, 674, 801, 809
AluI	AGCT	6 cut(s)	295, 415, 480, 674, 801, 809
Alw21I	GWGCWC	1 cut(s)	417
Alw26I	GTCTC	3 cut(s)	22, 150, 888
AlwI	GGATC	4 cut(s)	307, 382, 564, 923
Ama87I	CYCGRG	2 cut(s)	213, 392
Aor13HI	TCCGGA	1 cut(s)	80
AoxI	GGCC	3 cut(s)	196, 459, 750
ApoI	RAATTY	3 cut(s)	71, 191, 544
ArsI	GACNNNNNNTTYG	2 cut(s)	866, 898
AspS9I	GGNCC	1 cut(s)	147
AvaI	CYCGRG	2 cut(s)	213, 392
AvaII	GGWCC	1 cut(s)	147
BanII	GRGCYC	1 cut(s)	417
Bbv12I	GWGCWC	1 cut(s)	417
BbvCI	CCTCAGC	1 cut(s)	296
BccI	CCATC	2 cut(s)	301, 585
BceAI	ACGGC	2 cut(s)	370, 421
BciT130I	CCWGG	1 cut(s)	850
BciVI	GTATCC	1 cut(s)	760
BcoDI	GTCTC	3 cut(s)	22, 150, 888
BfaI	CTAG	1 cut(s)	638
BfuI	GTATCC	1 cut(s)	760
BglI	GCCNNNNNGGC	1 cut(s)	354
BisI	GCNGC	1 cut(s)	462
BlsI	GCNGC	1 cut(s)	463
Bme1390I	CCNGG	1 cut(s)	850
Bme18I	GGWCC	1 cut(s)	147
BmeT110I	CYCGRG	2 cut(s)	213, 392
BmgT120I	GGNCC	1 cut(s)	147
BmiI	GGNNCC	1 cut(s)	362
BmrFI	CCNGG	1 cut(s)	850
BmsI	GCATC	2 cut(s)	298, 387
BplI	GAGNNNNNCTC	2 cut(s)	868, 900
Bpu10I	CCTNAGC	2 cut(s)	296, 797
BpuEI	CTTGAG	2 cut(s)	36, 801
BsaAI	YACGTR	1 cut(s)	235
BsaBI	GATNNNNATC	1 cut(s)	311
BsaJI	CCNNGG	4 cut(s)	91, 178, 825, 908
BsaWI	WCCGGW	1 cut(s)	80
Bsc4I	CCNNNNNNNGG	2 cut(s)	184, 876
Bse1I	ACTGG	1 cut(s)	701
Bse8I	GATNNNNATC	1 cut(s)	311
BseAI	TCCGGA	1 cut(s)	80
BseBI	CCWGG	1 cut(s)	850
BseDI	CCNNGG	4 cut(s)	91, 178, 825, 908
BseGI	GGATG	2 cut(s)	339, 596
BseJI	GATNNNNATC	1 cut(s)	311
BseLI	CCNNNNNNNGG	2 cut(s)	184, 876
BseMII	CTCAG	2 cut(s)	287, 442
BseNI	ACTGG	1 cut(s)	701
BseRI	GAGGAG	2 cut(s)	313, 859
BseX3I	CGGCCG	1 cut(s)	196
BseYI	CCCAGC	1 cut(s)	805
Bsh1236I	CGCG	1 cut(s)	827
Bsh1285I	CGRYCG	2 cut(s)	199, 376
BshFI	GGCC	3 cut(s)	198, 461, 752
BsiEI	CGRYCG	2 cut(s)	199, 376
BsiHKAI	GWGCWC	1 cut(s)	417
BsiHKCI	CYCGRG	2 cut(s)	213, 392
BsiSI	CCGG	2 cut(s)	81, 936
BslFI	GGGAC	1 cut(s)	542
BslI	CCNNNNNNNGG	2 cut(s)	184, 876
BsmAI	GTCTC	3 cut(s)	22, 150, 888
BsmFI	GGGAC	1 cut(s)	542
BsnI	GGCC	3 cut(s)	198, 461, 752
BsoBI	CYCGRG	2 cut(s)	213, 392
Bsp1286I	GDGCHC	1 cut(s)	417
Bsp13I	TCCGGA	1 cut(s)	80
Bsp1407I	TGTACA	1 cut(s)	598
Bsp143I	GATC	7 cut(s)	165, 267, 312, 387, 556, 894, 928
BspACI	CCGC	8 cut(s)	122, 328, 346, 376, 408, 462, 825, 827
BspANI	GGCC	3 cut(s)	198, 461, 752
BspCNI	CTCAG	2 cut(s)	288, 441
BspEI	TCCGGA	1 cut(s)	80
BspFNI	CGCG	1 cut(s)	827
BspHI	TCATGA	2 cut(s)	559, 676
BspLI	GGNNCC	1 cut(s)	362
BspPI	GGATC	4 cut(s)	307, 382, 564, 923
BsrGI	TGTACA	1 cut(s)	598
BsrI	ACTGG	1 cut(s)	701
BssECI	CCNNGG	4 cut(s)	91, 178, 825, 908
BssMI	GATC	7 cut(s)	165, 267, 312, 387, 556, 894, 928
BssT1I	CCWWGG	1 cut(s)	908
Bst2UI	CCWGG	1 cut(s)	850
Bst4CI	ACNGT	1 cut(s)	603
Bst6I	CTCTTC	1 cut(s)	891
BstAUI	TGTACA	1 cut(s)	598
BstBAI	YACGTR	1 cut(s)	235
BstDEI	CTNAG	3 cut(s)	296, 428, 797
BstDSI	CCRYGG	2 cut(s)	91, 825
BstENI	CCTNNNNNAGG	2 cut(s)	182, 874
BstF5I	GGATG	2 cut(s)	339, 596
BstFNI	CGCG	1 cut(s)	827
BstKTI	GATC	7 cut(s)	168, 270, 315, 390, 559, 897, 931
BstMAI	GTCTC	3 cut(s)	22, 150, 888
BstMBI	GATC	7 cut(s)	165, 267, 312, 387, 556, 894, 928
BstMCI	CGRYCG	2 cut(s)	199, 376
BstMWI	GCNNNNNNNGC	3 cut(s)	218, 354, 714
BstNI	CCWGG	1 cut(s)	850
BstNSI	RCATGY	1 cut(s)	518
BstSCI	CCNGG	1 cut(s)	848
BstSNI	TACGTA	1 cut(s)	235
BstUI	CGCG	1 cut(s)	827
BstX2I	RGATCY	2 cut(s)	312, 928
BstYI	RGATCY	2 cut(s)	312, 928
BstZI	CGGCCG	1 cut(s)	196
BsuI	GTATCC	1 cut(s)	760
BsuRI	GGCC	3 cut(s)	198, 461, 752
BtgI	CCRYGG	2 cut(s)	91, 825
BtsCI	GGATG	2 cut(s)	339, 596
CciI	TCATGA	2 cut(s)	559, 676
Cfr13I	GGNCC	1 cut(s)	147
Cfr42I	CCGCGG	1 cut(s)	828
Csp6I	GTAC	4 cut(s)	232, 277, 397, 599
CviAII	CATG	5 cut(s)	515, 560, 625, 677, 898
CviQI	GTAC	4 cut(s)	232, 277, 397, 599
DdeI	CTNAG	3 cut(s)	296, 428, 797
DpnI	GATC	7 cut(s)	167, 269, 314, 389, 558, 896, 930
DpnII	GATC	7 cut(s)	165, 267, 312, 387, 556, 894, 928
EaeI	YGGCCR	1 cut(s)	196
EagI	CGGCCG	1 cut(s)	196
Eam1104I	CTCTTC	1 cut(s)	891
EarI	CTCTTC	1 cut(s)	891
EciI	GGCGGA	3 cut(s)	317, 335, 423
Ecl136II	GAGCTC	1 cut(s)	415
EclXI	CGGCCG	1 cut(s)	196
Eco105I	TACGTA	1 cut(s)	235
Eco130I	CCWWGG	1 cut(s)	908
Eco24I	GRGCYC	1 cut(s)	417
Eco47I	GGWCC	1 cut(s)	147
Eco52I	CGGCCG	1 cut(s)	196
Eco53kI	GAGCTC	1 cut(s)	415
Eco57I	CTGAAG	1 cut(s)	507
Eco88I	CYCGRG	2 cut(s)	213, 392
EcoICRI	GAGCTC	1 cut(s)	415
EcoNI	CCTNNNNNAGG	2 cut(s)	182, 874
EcoRI	GAATTC	1 cut(s)	71
EcoRII	CCWGG	1 cut(s)	848
EcoT14I	CCWWGG	1 cut(s)	908
EcoT38I	GRGCYC	1 cut(s)	417
ErhI	CCWWGG	1 cut(s)	908
FaeI	CATG	5 cut(s)	518, 563, 628, 680, 901
FaqI	GGGAC	1 cut(s)	542
FatI	CATG	5 cut(s)	514, 559, 624, 676, 897
FblI	GTMKAC	1 cut(s)	204
Fnu4HI	GCNGC	1 cut(s)	462
FokI	GGATG	2 cut(s)	326, 603
FriOI	GRGCYC	1 cut(s)	417
Fsp4HI	GCNGC	1 cut(s)	462
FspBI	CTAG	1 cut(s)	638
GluI	GCNGC	1 cut(s)	462
GsaI	CCCAGC	1 cut(s)	809
HaeIII	GGCC	3 cut(s)	198, 461, 752
HapII	CCGG	2 cut(s)	81, 936
Hin1II	CATG	5 cut(s)	518, 563, 628, 680, 901
HincII	GTYRAC	1 cut(s)	793
HindII	GTYRAC	1 cut(s)	793
HindIII	AAGCTT	1 cut(s)	799
HinfI	GANTC	6 cut(s)	104, 128, 201, 283, 520, 866
HpaII	CCGG	2 cut(s)	81, 936
Hpy166II	GTNNAC	2 cut(s)	205, 793
Hpy188I	TCNGA	3 cut(s)	170, 182, 451
Hpy8I	GTNNAC	2 cut(s)	205, 793
Hpy99I	CGWCG	1 cut(s)	212
HpyAV	CCTTC	2 cut(s)	241, 781
HpyCH4III	ACNGT	1 cut(s)	603
HpyCH4IV	ACGT	2 cut(s)	207, 234
HpyCH4V	TGCA	2 cut(s)	581, 945
HpyF10VI	GCNNNNNNNGC	3 cut(s)	218, 354, 714
HpyF3I	CTNAG	3 cut(s)	296, 428, 797
HpySE526I	ACGT	2 cut(s)	207, 234
Hsp92II	CATG	5 cut(s)	518, 563, 628, 680, 901
Kpn2I	TCCGGA	1 cut(s)	80
KspI	CCGCGG	1 cut(s)	828
Kzo9I	GATC	7 cut(s)	165, 267, 312, 387, 556, 894, 928
LmnI	GCTCC	1 cut(s)	262
LweI	GCATC	2 cut(s)	298, 387
MaeI	CTAG	1 cut(s)	638
MaeII	ACGT	2 cut(s)	207, 234
MaeIII	GTNAC	1 cut(s)	503
MalI	GATC	7 cut(s)	167, 269, 314, 389, 558, 896, 930
MboI	GATC	7 cut(s)	165, 267, 312, 387, 556, 894, 928
MboII	GAAGA	3 cut(s)	148, 373, 878
MflI	RGATCY	2 cut(s)	312, 928
MhlI	GDGCHC	1 cut(s)	417
MluCI	AATT	5 cut(s)	32, 71, 191, 544, 576
MlyI	GAGTC	2 cut(s)	210, 860
MroI	TCCGGA	1 cut(s)	80
MslI	CAYNNNNRTG	1 cut(s)	681
MspA1I	CMGCKG	3 cut(s)	295, 809, 827
MspI	CCGG	2 cut(s)	81, 936
MspR9I	CCNGG	1 cut(s)	850
MvaI	CCWGG	1 cut(s)	850
MvnI	CGCG	1 cut(s)	827
MwoI	GCNNNNNNNGC	3 cut(s)	218, 354, 714
NdeII	GATC	7 cut(s)	165, 267, 312, 387, 556, 894, 928
NlaIII	CATG	5 cut(s)	518, 563, 628, 680, 901
NlaIV	GGNNCC	1 cut(s)	362
NmeAIII	GCCGAG	1 cut(s)	224
NmuCI	GTSAC	1 cut(s)	503
NspI	RCATGY	1 cut(s)	518
PaeR7I	CTCGAG	1 cut(s)	392
PagI	TCATGA	2 cut(s)	559, 676
PfeI	GAWTC	4 cut(s)	104, 128, 283, 520
PkrI	GCNGC	1 cut(s)	463
PleI	GAGTC	2 cut(s)	209, 860
PpsI	GAGTC	2 cut(s)	209, 860
Ppu21I	YACGTR	1 cut(s)	235
Psp124BI	GAGCTC	1 cut(s)	417
Psp6I	CCWGG	1 cut(s)	848
PspFI	CCCAGC	1 cut(s)	805
PspGI	CCWGG	1 cut(s)	848
PspN4I	GGNNCC	1 cut(s)	362
PspPI	GGNCC	1 cut(s)	147
PspXI	VCTCGAGB	1 cut(s)	392
PsuI	RGATCY	2 cut(s)	312, 928
PvuII	CAGCTG	2 cut(s)	295, 809
RsaI	GTAC	4 cut(s)	233, 278, 398, 600
RsaNI	GTAC	4 cut(s)	232, 277, 397, 599
RseI	CAYNNNNRTG	1 cut(s)	681
SacI	GAGCTC	1 cut(s)	417
SacII	CCGCGG	1 cut(s)	828
SatI	GCNGC	1 cut(s)	462
Sau3AI	GATC	7 cut(s)	165, 267, 312, 387, 556, 894, 928
Sau96I	GGNCC	1 cut(s)	147
SchI	GAGTC	2 cut(s)	210, 860
ScrFI	CCNGG	1 cut(s)	850
SduI	GDGCHC	1 cut(s)	417
SfaNI	GCATC	2 cut(s)	298, 387
Sfr274I	CTCGAG	1 cut(s)	392
Sfr303I	CCGCGG	1 cut(s)	828
SgrBI	CCGCGG	1 cut(s)	828
SinI	GGWCC	1 cut(s)	147
SlaI	CTCGAG	1 cut(s)	392
SmiMI	CAYNNNNRTG	1 cut(s)	681
SmlI	CTYRAG	3 cut(s)	51, 392, 816
SmoI	CTYRAG	3 cut(s)	51, 392, 816
SnaBI	TACGTA	1 cut(s)	235
Sse9I	AATT	5 cut(s)	32, 71, 191, 544, 576
SsiI	CCGC	8 cut(s)	122, 328, 346, 376, 408, 462, 825, 827
SspI	AATATT	1 cut(s)	255
SspMI	CTAG	1 cut(s)	638
SstI	GAGCTC	1 cut(s)	417
StyD4I	CCNGG	1 cut(s)	848
StyI	CCWWGG	1 cut(s)	908
TaaI	ACNGT	1 cut(s)	603
TaiI	ACGT	2 cut(s)	210, 237
TaqI	TCGA	6 cut(s)	107, 134, 162, 270, 393, 568
TasI	AATT	5 cut(s)	32, 71, 191, 544, 576
TatI	WGTACW	2 cut(s)	276, 598
TauI	GCSGC	1 cut(s)	464
TfiI	GAWTC	4 cut(s)	104, 128, 283, 520
TseFI	GTSAC	1 cut(s)	503
Tsp45I	GTSAC	1 cut(s)	503
TspDTI	ATGAA	2 cut(s)	512, 800
TspGWI	ACGGA	1 cut(s)	435
VpaK11BI	GGWCC	1 cut(s)	147
XagI	CCTNNNNNAGG	2 cut(s)	182, 874
XapI	RAATTY	3 cut(s)	71, 191, 544
XbaI	TCTAGA	1 cut(s)	637
XceI	RCATGY	1 cut(s)	518
XhoI	CTCGAG	1 cut(s)	392
XmiI	GTMKAC	1 cut(s)	204
XspI	CTAG	1 cut(s)	638

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.