Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0003316

Physical Location & Seq

Genomic Coordinates Reverse (-)

8348315 .. 8350562

Gene Structure 2248 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG046000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 966 bp

ATGAAAATTCAAATTTCTGCAGATAAAAATCAAACACTCGTGGCTTCTCAGATTTCAAAATCCAATCTTTCCCTTCTCTCAGTTTCTCTGGTTTTTTTCAAATCCCACCCAGAAATCCATCTCATGGCGACGAAATCCGATTCCCCAAAGAGGGAGTGGTCCCTGAACGACTTCGAGATCGGAAAGCCTCTCGGCAAAGGCAAATTCGGGCGGGTCTACGTGGCCCGAGAAGTCAAGAGCAAGTACGTGGTGGCGTTGAAGGTGTTGTTCAAGAAGCAGATAAAGAAGTACAAGATTCACCACCAGCTGAAGAGAGAGATGGAGATCCAGACCAGTCTCAGACACCCCAACATTCTTCGCCTCTACGGTTGGTTCCACGACGACGAACGCATCGTCTTGATCCTCGAGTACGCCCACGGCGGCGAGCTGTATGGGCTGCTCAGGGAGAACACTCACCTCTCTGAGAAACAGACCGCCACGTATATACTGAGCCTGGCGCAGGCTTTGGCTTATTGTCACGAGAAGCATGTGATTCATAGGGATATCAAGCCGGAAAATTTGTTGCTTGATCATGAGGGTCGACTGAAAATTGCAGACTTTGGATGGTCTGTACAGTCAAGCAGCAAGAGACGAACAATGTGTGGAACGTTAGATTATTTAGCACCAGAAATGGTGGAGAACAGAGCTCATGATTATGCAGTGGATAACTGGACTTTGGGAATTCTCTGCTATGAGTTTCTTTATGGCACTCCTCCATTTGAGGCTGAAAGTCAAAGAGATACTTTCAAAAGGATTACGAACATTGACCTGAGCTTTCCTTCTAGCCCTCAAGTTTCTGCAGAATCTAAACATCTCATTACCCAGCTTCTCGTGAAGGACTCCTCTAAGAGGCTTTCTCTTCAGAAGATCATGGAACACCCTTGGATAATGAAGAATGCAGATCCTTCCGGTATCTGCAATAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000228 GO:0000278 GO:0000775 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0005875 GO:0005876 GO:0006325 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0008150 GO:0008152 GO:0009987 GO:0010564 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016569 GO:0016570 GO:0016572 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022402 GO:0031974 GO:0031981 GO:0032133 GO:0032465 GO:0032991 GO:0035173 GO:0035174 GO:0035175 GO:0035404 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043987 GO:0043988 GO:0044022 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0048471 GO:0050789 GO:0050794 GO:0051233 GO:0051276 GO:0051302 GO:0051726 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0098687 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140096 GO:1901564 GO:1902850 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

321

Amino Acids

36.99

Weight (kDa)

9.19

Isoelectric Point (pI)

35.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	58 - 309	2.6e-76	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	59 - 305	5.2e-45	Protein tyrosine and serine/threonine kinase
Kinase-like	PF14531	149 - 253	1e-06	Kinase-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003316)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45490 AT2G45490
fragaria_vesca	FvH4_4g03900 FvH4_4g03900 FvH4_4g03900 FvH4_4g03900 FvH4_4g03900 FvH4_7g12170 FvH4_7g12170 FvH4_7g12170 FvH4_7g12170
malus_domestica	MD02G1200900.v1.1
prunus_persica	Prupe.2G148200_v2.0.a1 Prupe.2G148200_v2.0.a1
pyrus_communis	pycom02g16420
rosa_chinensis	RchiOBHm_Chr1g0350921 RchiOBHm_Chr4g0393931
rosa_laevigata	RLG00000028441
rosa_multiflora	Rmu_sc0002665.1_g000020 Rmu_sc0014584.1_g000001
rosa_roxburghii	Rroxscaffold_4G00304300 Rroxscaffold_5G00339010
rosa_rugosa	Rorug01G0215300 Rorug03G0370400 Rorug03G0370500
rosa_samantha	Rh1AG230000 Rh1BG199900 Rh1CG214400 Rh1DG227700 Rh4AG049600 Rh4BG046000 Rh4CG053100 Rh4DG046600
rosa_wichuraiana	Rw1G019910 Rw1G019940 Rw4G003930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	124
AccI	GTMKAC	2 cut(s)	216, 580
AciI	CCGC	3 cut(s)	211, 420, 474
AclI	AACGTT	1 cut(s)	647
AclWI	GGATC	3 cut(s)	319, 394, 935
AcsI	RAATTY	5 cut(s)	6, 12, 203, 556, 720
AcuI	CTGAAG	2 cut(s)	329, 884
AfaI	GTAC	4 cut(s)	245, 290, 410, 612
AfiI	CCNNNNNNNGG	5 cut(s)	124, 150, 151, 499, 888
AgsI	TTSAA	6 cut(s)	11, 57, 100, 259, 271, 787
AjnI	CCWGG	1 cut(s)	492
AluBI	AGCT	5 cut(s)	307, 427, 686, 813, 865
AluI	AGCT	5 cut(s)	307, 427, 686, 813, 865
Alw21I	GWGCWC	1 cut(s)	688
Alw26I	GTCTC	2 cut(s)	341, 622
AlwI	GGATC	3 cut(s)	319, 394, 935
Ama87I	CYCGRG	2 cut(s)	225, 404
AoxI	GGCC	1 cut(s)	222
ApeKI	GCWGC	2 cut(s)	436, 621
ApoI	RAATTY	5 cut(s)	6, 12, 203, 556, 720
ArsI	GACNNNNNNTTYG	2 cut(s)	378, 410
Asp700I	GAANNNNTTC	1 cut(s)	170
AspLEI	GCGC	1 cut(s)	499
AspS9I	GGNCC	2 cut(s)	159, 223
AsuHPI	GGTGA	2 cut(s)	290, 446
AvaI	CYCGRG	2 cut(s)	225, 404
AvaII	GGWCC	1 cut(s)	159
BanII	GRGCYC	1 cut(s)	688
BauI	CACGAG	3 cut(s)	38, 518, 869
Bbv12I	GWGCWC	1 cut(s)	688
BbvI	GCAGC	2 cut(s)	423, 633
BccI	CCATC	3 cut(s)	126, 313, 597
BceAI	ACGGC	1 cut(s)	433
BciT130I	CCWGG	1 cut(s)	494
BclI	TGATCA	1 cut(s)	568
BcoDI	GTCTC	2 cut(s)	341, 622
BfaI	CTAG	2 cut(s)	822, 964
BfmI	CTRYAG	2 cut(s)	18, 837
BisI	GCNGC	3 cut(s)	421, 437, 622
BlsI	GCNGC	3 cut(s)	422, 438, 623
Bme1390I	CCNGG	1 cut(s)	494
Bme18I	GGWCC	1 cut(s)	159
BmeT110I	CYCGRG	2 cut(s)	225, 404
BmgT120I	GGNCC	2 cut(s)	159, 223
BmiI	GGNNCC	2 cut(s)	161, 374
BmrFI	CCNGG	1 cut(s)	494
BmsI	GCATC	1 cut(s)	399
BplI	GAGNNNNNCTC	2 cut(s)	880, 912
Bpu10I	CCTNAGC	2 cut(s)	440, 809
BpuEI	CTTGAG	1 cut(s)	813
BsaAI	YACGTR	3 cut(s)	220, 247, 480
BsaBI	GATNNNNATC	2 cut(s)	27, 323
BsaJI	CCNNGG	2 cut(s)	415, 920
BsaWI	WCCGGW	1 cut(s)	947
Bsc4I	CCNNNNNNNGG	5 cut(s)	124, 150, 151, 499, 888
Bse1I	ACTGG	2 cut(s)	333, 713
Bse8I	GATNNNNATC	2 cut(s)	27, 323
BseBI	CCWGG	1 cut(s)	494
BseDI	CCNNGG	2 cut(s)	415, 920
BseGI	GGATG	1 cut(s)	608
BseJI	GATNNNNATC	2 cut(s)	27, 323
BseLI	CCNNNNNNNGG	5 cut(s)	124, 150, 151, 499, 888
BseMII	CTCAG	7 cut(s)	62, 93, 352, 453, 454, 479, 800
BseNI	ACTGG	2 cut(s)	333, 713
BseRI	GAGGAG	2 cut(s)	741, 871
BseXI	GCAGC	2 cut(s)	423, 633
BseYI	CCCAGC	1 cut(s)	861
BshFI	GGCC	1 cut(s)	224
BsiHKAI	GWGCWC	1 cut(s)	688
BsiHKCI	CYCGRG	2 cut(s)	225, 404
BsiSI	CCGG	2 cut(s)	551, 948
BslFI	GGGAC	1 cut(s)	145
BslI	CCNNNNNNNGG	5 cut(s)	124, 150, 151, 499, 888
BsmAI	GTCTC	2 cut(s)	341, 622
BsmBI	CGTCTC	1 cut(s)	622
BsmFI	GGGAC	1 cut(s)	145
BsmI	GAATGC	1 cut(s)	940
BsnI	GGCC	1 cut(s)	224
BsoBI	CYCGRG	2 cut(s)	225, 404
Bsp1286I	GDGCHC	1 cut(s)	688
Bsp1407I	TGTACA	1 cut(s)	610
Bsp143I	GATC	6 cut(s)	177, 324, 399, 568, 906, 940
BspACI	CCGC	3 cut(s)	211, 420, 474
BspANI	GGCC	1 cut(s)	224
BspCNI	CTCAG	7 cut(s)	61, 92, 351, 453, 454, 480, 801
BspHI	TCATGA	2 cut(s)	571, 688
BspLI	GGNNCC	2 cut(s)	161, 374
BspMAI	CTGCAG	2 cut(s)	22, 841
BspPI	GGATC	3 cut(s)	319, 394, 935
BsrGI	TGTACA	1 cut(s)	610
BsrI	ACTGG	2 cut(s)	333, 713
BssECI	CCNNGG	2 cut(s)	415, 920
BssMI	GATC	6 cut(s)	177, 324, 399, 568, 906, 940
BssSI	CACGAG	3 cut(s)	38, 518, 869
BssT1I	CCWWGG	1 cut(s)	920
Bst2BI	CACGAG	3 cut(s)	38, 518, 869
Bst2UI	CCWGG	1 cut(s)	494
Bst4CI	ACNGT	2 cut(s)	368, 615
Bst6I	CTCTTC	2 cut(s)	305, 903
BstAUI	TGTACA	1 cut(s)	610
BstBAI	YACGTR	3 cut(s)	220, 247, 480
BstC8I	GCNNGC	2 cut(s)	425, 501
BstDEI	CTNAG	8 cut(s)	48, 79, 338, 440, 462, 488, 809, 885
BstDSI	CCRYGG	1 cut(s)	415
BstENI	CCTNNNNNAGG	2 cut(s)	497, 886
BstF5I	GGATG	1 cut(s)	608
BstHHI	GCGC	1 cut(s)	499
BstKTI	GATC	6 cut(s)	180, 327, 402, 571, 909, 943
BstMAI	GTCTC	2 cut(s)	341, 622
BstMBI	GATC	6 cut(s)	177, 324, 399, 568, 906, 940
BstMWI	GCNNNNNNNGC	1 cut(s)	433
BstNI	CCWGG	1 cut(s)	494
BstNSI	RCATGY	1 cut(s)	530
BstSCI	CCNGG	1 cut(s)	492
BstSFI	CTRYAG	2 cut(s)	18, 837
BstV1I	GCAGC	2 cut(s)	423, 633
BstX2I	RGATCY	2 cut(s)	324, 940
BstYI	RGATCY	2 cut(s)	324, 940
BsuRI	GGCC	1 cut(s)	224
BtgI	CCRYGG	1 cut(s)	415
BtsCI	GGATG	1 cut(s)	608
BtsI	GCAGTG	1 cut(s)	705
BtsIMutI	CAGTG	1 cut(s)	705
Cac8I	GCNNGC	2 cut(s)	425, 501
CciI	TCATGA	2 cut(s)	571, 688
CfoI	GCGC	1 cut(s)	499
Cfr13I	GGNCC	2 cut(s)	159, 223
Csp6I	GTAC	4 cut(s)	244, 289, 409, 611
CviAII	CATG	5 cut(s)	124, 527, 572, 689, 910
CviQI	GTAC	4 cut(s)	244, 289, 409, 611
DdeI	CTNAG	8 cut(s)	48, 79, 338, 440, 462, 488, 809, 885
DpnI	GATC	6 cut(s)	179, 326, 401, 570, 908, 942
DpnII	GATC	6 cut(s)	177, 324, 399, 568, 906, 940
Eam1104I	CTCTTC	2 cut(s)	305, 903
EarI	CTCTTC	2 cut(s)	305, 903
Ecl136II	GAGCTC	1 cut(s)	686
Eco130I	CCWWGG	1 cut(s)	920
Eco24I	GRGCYC	1 cut(s)	688
Eco32I	GATATC	1 cut(s)	544
Eco47I	GGWCC	1 cut(s)	159
Eco53kI	GAGCTC	1 cut(s)	686
Eco57I	CTGAAG	2 cut(s)	329, 884
Eco88I	CYCGRG	2 cut(s)	225, 404
EcoICRI	GAGCTC	1 cut(s)	686
EcoNI	CCTNNNNNAGG	2 cut(s)	497, 886
EcoRI	GAATTC	1 cut(s)	720
EcoRII	CCWGG	1 cut(s)	492
EcoRV	GATATC	1 cut(s)	544
EcoT14I	CCWWGG	1 cut(s)	920
EcoT38I	GRGCYC	1 cut(s)	688
ErhI	CCWWGG	1 cut(s)	920
Esp3I	CGTCTC	1 cut(s)	622
FaeI	CATG	5 cut(s)	127, 530, 575, 692, 913
FalI	AAGNNNNNCTT	2 cut(s)	766, 798
FaqI	GGGAC	1 cut(s)	145
FatI	CATG	5 cut(s)	123, 526, 571, 688, 909
FauI	CCCGC	1 cut(s)	204
FbaI	TGATCA	1 cut(s)	568
FblI	GTMKAC	2 cut(s)	216, 580
Fnu4HI	GCNGC	3 cut(s)	421, 437, 622
FokI	GGATG	1 cut(s)	615
FriOI	GRGCYC	1 cut(s)	688
Fsp4HI	GCNGC	3 cut(s)	421, 437, 622
FspBI	CTAG	2 cut(s)	822, 964
GlaI	GCGC	1 cut(s)	498
GluI	GCNGC	3 cut(s)	421, 437, 622
GsaI	CCCAGC	1 cut(s)	865
HaeIII	GGCC	1 cut(s)	224
HapII	CCGG	2 cut(s)	551, 948
HhaI	GCGC	1 cut(s)	499
Hin1II	CATG	5 cut(s)	127, 530, 575, 692, 913
Hin6I	GCGC	1 cut(s)	497
HinP1I	GCGC	1 cut(s)	497
HincII	GTYRAC	1 cut(s)	581
HindII	GTYRAC	1 cut(s)	581
HinfI	GANTC	5 cut(s)	140, 295, 532, 842, 878
HpaII	CCGG	2 cut(s)	551, 948
HphI	GGTGA	2 cut(s)	290, 446
Hpy166II	GTNNAC	2 cut(s)	217, 581
Hpy188I	TCNGA	6 cut(s)	51, 139, 182, 341, 463, 903
Hpy188III	TCNNGA	9 cut(s)	175, 235, 271, 328, 397, 518, 572, 689, 871
Hpy8I	GTNNAC	2 cut(s)	217, 581
Hpy99I	CGWCG	3 cut(s)	133, 383, 386
HpyAV	CCTTC	5 cut(s)	83, 253, 828, 868, 954
HpyCH4III	ACNGT	2 cut(s)	368, 615
HpyCH4IV	ACGT	4 cut(s)	219, 246, 479, 647
HpyCH4V	TGCA	6 cut(s)	20, 593, 698, 839, 938, 957
HpyF10VI	GCNNNNNNNGC	1 cut(s)	433
HpyF3I	CTNAG	8 cut(s)	48, 79, 338, 440, 462, 488, 809, 885
HpySE526I	ACGT	4 cut(s)	219, 246, 479, 647
Hsp92II	CATG	5 cut(s)	127, 530, 575, 692, 913
HspAI	GCGC	1 cut(s)	497
Ksp22I	TGATCA	1 cut(s)	568
Kzo9I	GATC	6 cut(s)	177, 324, 399, 568, 906, 940
Lsp1109I	GCAGC	2 cut(s)	423, 633
LweI	GCATC	1 cut(s)	399
MaeI	CTAG	2 cut(s)	822, 964
MaeII	ACGT	4 cut(s)	219, 246, 479, 647
MaeIII	GTNAC	1 cut(s)	515
MalI	GATC	6 cut(s)	179, 326, 401, 570, 908, 942
MboI	GATC	6 cut(s)	177, 324, 399, 568, 906, 940
MboII	GAAGA	5 cut(s)	322, 347, 890, 916, 943
MflI	RGATCY	2 cut(s)	324, 940
MhlI	GDGCHC	1 cut(s)	688
MluCI	AATT	6 cut(s)	6, 12, 203, 556, 588, 720
MlyI	GAGTC	1 cut(s)	872
MroXI	GAANNNNTTC	1 cut(s)	170
MslI	CAYNNNNRTG	1 cut(s)	693
MspA1I	CMGCKG	1 cut(s)	307
MspI	CCGG	2 cut(s)	551, 948
MspR9I	CCNGG	1 cut(s)	494
Mva1269I	GAATGC	1 cut(s)	940
MvaI	CCWGG	1 cut(s)	494
MwoI	GCNNNNNNNGC	1 cut(s)	433
NdeII	GATC	6 cut(s)	177, 324, 399, 568, 906, 940
NlaIII	CATG	5 cut(s)	127, 530, 575, 692, 913
NlaIV	GGNNCC	2 cut(s)	161, 374
NmeAIII	GCCGAG	1 cut(s)	171
NmuCI	GTSAC	1 cut(s)	515
NspI	RCATGY	1 cut(s)	530
PaeR7I	CTCGAG	1 cut(s)	404
PagI	TCATGA	2 cut(s)	571, 688
PcsI	WCGNNNNNNNCGW	1 cut(s)	390
PctI	GAATGC	1 cut(s)	940
PdmI	GAANNNNTTC	1 cut(s)	170
PfeI	GAWTC	4 cut(s)	140, 295, 532, 842
PflMI	CCANNNNNTGG	1 cut(s)	124
PkrI	GCNGC	3 cut(s)	422, 438, 623
PleI	GAGTC	1 cut(s)	872
PpsI	GAGTC	1 cut(s)	872
Ppu21I	YACGTR	3 cut(s)	220, 247, 480
Psp124BI	GAGCTC	1 cut(s)	688
Psp1406I	AACGTT	1 cut(s)	647
Psp6I	CCWGG	1 cut(s)	492
PspFI	CCCAGC	1 cut(s)	861
PspGI	CCWGG	1 cut(s)	492
PspN4I	GGNNCC	2 cut(s)	161, 374
PspPI	GGNCC	2 cut(s)	159, 223
PspXI	VCTCGAGB	1 cut(s)	404
PstI	CTGCAG	2 cut(s)	22, 841
PsuI	RGATCY	2 cut(s)	324, 940
PvuII	CAGCTG	1 cut(s)	307
RsaI	GTAC	4 cut(s)	245, 290, 410, 612
RsaNI	GTAC	4 cut(s)	244, 289, 409, 611
RseI	CAYNNNNRTG	1 cut(s)	693
SacI	GAGCTC	1 cut(s)	688
SalI	GTCGAC	1 cut(s)	579
SatI	GCNGC	3 cut(s)	421, 437, 622
Sau3AI	GATC	6 cut(s)	177, 324, 399, 568, 906, 940
Sau96I	GGNCC	2 cut(s)	159, 223
SchI	GAGTC	1 cut(s)	872
ScrFI	CCNGG	1 cut(s)	494
SduI	GDGCHC	1 cut(s)	688
SfaNI	GCATC	1 cut(s)	399
SfcI	CTRYAG	2 cut(s)	18, 837
Sfr274I	CTCGAG	1 cut(s)	404
SinI	GGWCC	1 cut(s)	159
SlaI	CTCGAG	1 cut(s)	404
SmiMI	CAYNNNNRTG	1 cut(s)	693
SmlI	CTYRAG	2 cut(s)	404, 828
SmoI	CTYRAG	2 cut(s)	404, 828
Sse9I	AATT	6 cut(s)	6, 12, 203, 556, 588, 720
SsiI	CCGC	3 cut(s)	211, 420, 474
SspMI	CTAG	2 cut(s)	822, 964
SstI	GAGCTC	1 cut(s)	688
StyD4I	CCNGG	1 cut(s)	492
StyI	CCWWGG	1 cut(s)	920
TaaI	ACNGT	2 cut(s)	368, 615
TaiI	ACGT	4 cut(s)	222, 249, 482, 650
TaqI	TCGA	3 cut(s)	174, 405, 580
TasI	AATT	6 cut(s)	6, 12, 203, 556, 588, 720
TatI	WGTACW	2 cut(s)	288, 610
TauI	GCSGC	1 cut(s)	423
TfiI	GAWTC	4 cut(s)	140, 295, 532, 842
TscAI	CASTG	1 cut(s)	705
TseFI	GTSAC	1 cut(s)	515
TseI	GCWGC	2 cut(s)	436, 621
Tsp45I	GTSAC	1 cut(s)	515
TspDTI	ATGAA	3 cut(s)	17, 524, 944
TspRI	CASTG	1 cut(s)	705
Van91I	CCANNNNNTGG	1 cut(s)	124
VpaK11BI	GGWCC	1 cut(s)	159
XagI	CCTNNNNNAGG	2 cut(s)	497, 886
XapI	RAATTY	5 cut(s)	6, 12, 203, 556, 720
XceI	RCATGY	1 cut(s)	530
XcmI	CCANNNNNNNNNTGG	1 cut(s)	153
XhoI	CTCGAG	1 cut(s)	404
XmiI	GTMKAC	2 cut(s)	216, 580
XmnI	GAANNNNTTC	1 cut(s)	170
XspI	CTAG	2 cut(s)	822, 964

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.