Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001232

Physical Location & Seq

Genomic Coordinates Forward (+)

29215858 .. 29220262

Gene Structure 4405 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1242000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1767 bp

ATGGGGAAAGTATCTGAGGAACACATTGAAGCTCGCGTCAGACAACTCCTGTCTCGTATGACTTTGAAAGAAAAGGTTGGCCAGATGACCCAAATTGAGCGCCAAGTCGCCACTCCAGCTGCCATTAGAGACAACTCCATAGGGAGTATATTCAGTGCTCCGGGCAACGTGCCCTGTGAAAAGGCTTTGCAGTCAGAGTGGGCGGATATGGTGGATGGGCTTCAGAGGTCAGCGCTATCGTCGCGGCTTGGGATTCCCCTCATATATGGGACTGATGCGGTTCATGGCAACGGTAATGCTTATGGTGCCTACTATATTCCCTCACAACATGCAGATTTGGCTCGAAGGATTGGTGTGGCAACTGCTCTTGAAACCAGGGCTTGTGGTTTTCACTATACTTTCGCTCCGTGTGTGGCTGTATGCAAGGATCCGAGATGGGGAAGATGCTATGAGAGTTACAGTGAAGATACTGAAATTGTAAGCAAGATGACGTCTATTGTTTCAGGCTTGCAGGGGCAGCCTCCCCAAGGACATCCGAAGGGCTACCCTTTTATGGCGGGAAGCAACAACACTATTGCATCTGCCAAGCATTTTGTTGGAGAAGGGGGCACAGACAAAGGTTTAAATGAGGGGAATGCTGTATTATCTTACGAGGATTTAGAGAGGATTCATATCAAACCTTATCTGGACTGTATTTCTCAAGGAGTTTGTACTATTATGGTTTCCTATAACAGCTGGAATGGAGATAGAGTACATGGTCACCGTTTTCTCCTTACAGAAATCTTGAAAGATAAGCTAGGTTTTAAGGGAATTTTGATTTCTGACTGGGAAGGAATTGACCAACTTTGCGAACCTGAAGGCACAGACTATCGTTTATGCATTTCCTTAGCCATTAATGCCGGAATAGACATGGTCATGGTACCTTTCAGATATGAAAAATTCATTAAGGAATTAATATATCTTGTGGAATCCAGGGAGGTACCAATGTCTAGGATAGATGATGCTATTGCAAGAATACTAAGAGTGAAGTTTGTTGCTGGTGTTTTCGAACATCCCTTTGCTGATAAATCTTTACAGGACGCAGTTGGTTGCAAGTTGCACAGAGATCTAGCACGTGAAGCAGTTCGCAGGTCATTGGTTCTGTTGAAGAACGGAAAGGACCCAATGAAACCTTTTCTTCCATTAGACAGAAATGCTAAAAGAATACTTGTTACTGGAACTCATGCCGATGATCTTGGAAATCAATGTGGAGGATGGACGGCTACAAAATATGGTAGCAGCCGCCAGATAACAATCGGGACAACCATCTTGGAAGCTATTAAGATGGCAGTTGGAGAAGATACAGAAATAATTTACGAGAAATATTCGTCAGCAGACACCTTAGCGCGTCAAGATATCTCTTTTGCGATTGTGGTTGTTGGTGAAGCTCCGTATGCAGAAGGCAGGGGTTACAGTTCAGAGCTTGTGATCCCGTTCAATGGAACTGATGTCATAAGCTCGACTGCTGACAGAATTCCCGTCTTGGCGATTCTGATTTCTGGAAGACCCTTAGTTTTAGAGCCCTGGCTCTTGGAAAAGATGGATGCTCTTGTTGCTGCTTGGCTTCCTGGTACTGAAGGAGAGGGAATTGTAGATGCAATCTTTGGAGATTTTGACTTCGAGGGTAGACTCCCGGTGACATGGTTTAAAAGGGTTGAACAACTGCCTATGCATGCTGCAGACCCTTTATATCCTCTTGGCTTTGGGATGACATACAATAAGGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005886 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0015926 GO:0016020 GO:0016052 GO:0016787 GO:0016798 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:0071944 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

589

Amino Acids

64.79

Weight (kDa)

5.72

Isoelectric Point (pI)

35.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	21 - 342	1.6e-62	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	379 - 585	5.9e-37	Glycosyl hydrolase family 3 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001232)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G47000 AT3G47000 AT3G47000 AT3G47010 AT3G47010 AT3G47010 AT3G47040 AT3G47040 AT3G47040 AT3G47050
fragaria_vesca	FvH4_3g38160 FvH4_3g38160 FvH4_4g11940
malus_domestica	MD02G1242000.v1.1 MD02G1242100.v1.1 MD02G1242200.v1.1 MD02G1242300.v1.1 MD11G1080700.v1.1
prunus_persica	Prupe.2G092300_v2.0.a1 Prupe.2G092300_v2.0.a1 Prupe.2G092300_v2.0.a1 Prupe.2G092600_v2.0.a1 Prupe.2G092900_v2.0.a1 Prupe.2G092900_v2.0.a1 Prupe.6G061000_v2.0.a1 Prupe.6G061000_v2.0.a1 Prupe.6G061000_v2.0.a1 Prupe.6G061000_v2.0.a1 Prupe.6G061000_v2.0.a1
pyrus_communis	pycom02g20900 pycom11g06800 pycom11g06860
rosa_chinensis	RchiOBHm_Chr5g0068591 RchiOBHm_Chr6g0246121
rosa_laevigata	RLG00000035983 RLG00000036820 RLG00000036839
rosa_multiflora	Rmu_sc0004721.1_g000003
rosa_roxburghii	Rroxscaffold_1G00003560 Rroxscaffold_1G00012260 Rroxscaffold_3G00247860 Rroxscaffold_7G00214870
rosa_rugosa	Rorug05G0391700 Rorug05G0464600 Rorug05G0465200 Rorug05G0465800
rosa_samantha	Rh5AG449400 Rh5BG467600 Rh5DG481000 Rh5DG551200 Rh5DG552100 Rh7AG258000 Rh7CG274700 Rh7DG265200
rosa_wichuraiana	Rw5G041960 Rw5G048090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	494
Acc36I	ACCTGC	1 cut(s)	1119
Acc65I	GGTACC	2 cut(s)	919, 979
AccB1I	GGYRCC	3 cut(s)	305, 919, 979
AccI	GTMKAC	1 cut(s)	1666
AccII	CGCG	3 cut(s)	36, 244, 1387
AciI	CCGC	5 cut(s)	203, 244, 278, 557, 1282
AclWI	GGATC	3 cut(s)	422, 435, 1462
AcoI	YGGCCR	1 cut(s)	79
AcsI	RAATTY	3 cut(s)	810, 938, 1512
AcuI	CTGAAG	3 cut(s)	206, 876, 1635
AcvI	CACGTG	1 cut(s)	1115
AcyI	GRCGYC	1 cut(s)	491
AfaI	GTAC	5 cut(s)	712, 753, 921, 981, 1612
AfeI	AGCGCT	1 cut(s)	234
AfiI	CCNNNNNNNGG	4 cut(s)	437, 527, 553, 1478
AgsI	TTSAA	7 cut(s)	29, 67, 371, 787, 1147, 1477, 1697
AjnI	CCWGG	4 cut(s)	374, 971, 1562, 1606
AluBI	AGCT	8 cut(s)	32, 119, 735, 796, 1316, 1427, 1462, 1497
AluI	AGCT	8 cut(s)	32, 119, 735, 796, 1316, 1427, 1462, 1497
Alw21I	GWGCWC	1 cut(s)	160
Alw26I	GTCTC	2 cut(s)	57, 123
AlwI	GGATC	3 cut(s)	422, 435, 1462
Aor51HI	AGCGCT	1 cut(s)	234
AoxI	GGCC	1 cut(s)	79
ApeKI	GCWGC	5 cut(s)	119, 517, 1278, 1595, 1715
ApoI	RAATTY	3 cut(s)	810, 938, 1512
AseI	ATTAAT	2 cut(s)	894, 953
Asp700I	GAANNNNTTC	1 cut(s)	1122
Asp718I	GGTACC	2 cut(s)	919, 979
AspLEI	GCGC	3 cut(s)	102, 235, 1387
AspS9I	GGNCC	1 cut(s)	1159
AsuC2I	CCSGG	2 cut(s)	162, 1673
AsuHPI	GGTGA	3 cut(s)	752, 1433, 1687
AsuII	TTCGAA	1 cut(s)	1047
AvaII	GGWCC	1 cut(s)	1159
BaeGI	GKGCMC	2 cut(s)	174, 611
BaeI	ACNNNNGTAYC	2 cut(s)	971, 1004
BalI	TGGCCA	1 cut(s)	81
BamHI	GGATCC	1 cut(s)	427
BanI	GGYRCC	3 cut(s)	305, 919, 979
BanII	GRGCYC	1 cut(s)	1563
BbrPI	CACGTG	1 cut(s)	1115
BbsI	GAAGAC	1 cut(s)	1549
Bbv12I	GWGCWC	1 cut(s)	160
BbvI	GCAGC	5 cut(s)	106, 529, 1290, 1582, 1702
BccI	CCATC	6 cut(s)	209, 429, 1248, 1313, 1318, 1573
BceAI	ACGGC	1 cut(s)	1275
BciT130I	CCWGG	4 cut(s)	376, 973, 1564, 1608
BcnI	CCSGG	2 cut(s)	162, 1673
BcoDI	GTCTC	2 cut(s)	57, 123
BfaI	CTAG	3 cut(s)	797, 990, 1109
BfmI	CTRYAG	1 cut(s)	1716
BfoI	RGCGCY	2 cut(s)	103, 236
BfuAI	ACCTGC	1 cut(s)	1119
BglII	AGATCT	1 cut(s)	1105
BisI	GCNGC	7 cut(s)	120, 245, 518, 1279, 1282, 1596, 1716
BlsI	GCNGC	7 cut(s)	121, 246, 519, 1280, 1283, 1597, 1717
Bme1390I	CCNGG	6 cut(s)	162, 376, 973, 1564, 1608, 1673
Bme18I	GGWCC	1 cut(s)	1159
BmgT120I	GGNCC	1 cut(s)	1159
BmiI	GGNNCC	5 cut(s)	307, 429, 921, 981, 1161
BmrFI	CCNGG	6 cut(s)	162, 376, 973, 1564, 1608, 1673
BmrI	ACTGGG	1 cut(s)	835
BmsI	GCATC	6 cut(s)	265, 434, 587, 991, 1573, 1624
BmuI	ACTGGG	1 cut(s)	835
BpiI	GAAGAC	1 cut(s)	1549
BpmI	CTGGAG	1 cut(s)	99
Bpu10I	CCTNAGC	2 cut(s)	886, 1381
Bpu14I	TTCGAA	1 cut(s)	1047
BpuEI	CTTGAG	1 cut(s)	684
BpuMI	CCSGG	2 cut(s)	162, 1673
BsaAI	YACGTR	1 cut(s)	1115
BsaBI	GATNNNNATC	2 cut(s)	671, 1292
BsaHI	GRCGYC	1 cut(s)	491
BsaJI	CCNNGG	4 cut(s)	375, 526, 972, 1562
BsaXI	ACNNNNNCTCC	4 cut(s)	388, 418, 735, 765
Bsc4I	CCNNNNNNNGG	4 cut(s)	437, 527, 553, 1478
Bse1I	ACTGG	2 cut(s)	830, 1219
Bse8I	GATNNNNATC	2 cut(s)	671, 1292
BseBI	CCWGG	4 cut(s)	376, 973, 1564, 1608
BseDI	CCNNGG	4 cut(s)	375, 526, 972, 1562
BseGI	GGATG	6 cut(s)	220, 532, 1051, 1259, 1588, 1752
BseJI	GATNNNNATC	2 cut(s)	671, 1292
BseLI	CCNNNNNNNGG	4 cut(s)	437, 527, 553, 1478
BseMII	CTCAG	1 cut(s)	6
BseNI	ACTGG	2 cut(s)	830, 1219
BseSI	GKGCMC	2 cut(s)	174, 611
BseXI	GCAGC	5 cut(s)	106, 529, 1290, 1582, 1702
Bsh1236I	CGCG	3 cut(s)	36, 244, 1387
BshFI	GGCC	1 cut(s)	81
BshNI	GGYRCC	3 cut(s)	305, 919, 979
BsiHKAI	GWGCWC	1 cut(s)	160
BsiSI	CCGG	3 cut(s)	161, 900, 1673
BslFI	GGGAC	2 cut(s)	283, 1312
BslI	CCNNNNNNNGG	4 cut(s)	437, 527, 553, 1478
BsmAI	GTCTC	2 cut(s)	57, 123
BsmFI	GGGAC	2 cut(s)	283, 1312
BsmI	GAATGC	1 cut(s)	640
BsnI	GGCC	1 cut(s)	81
Bsp119I	TTCGAA	1 cut(s)	1047
Bsp1286I	GDGCHC	4 cut(s)	160, 174, 611, 1563
Bsp143I	GATC	4 cut(s)	427, 1105, 1231, 1467
BspACI	CCGC	5 cut(s)	203, 244, 278, 557, 1282
BspANI	GGCC	1 cut(s)	81
BspCNI	CTCAG	1 cut(s)	7
BspFNI	CGCG	3 cut(s)	36, 244, 1387
BspLI	GGNNCC	5 cut(s)	307, 429, 921, 981, 1161
BspMAI	CTGCAG	1 cut(s)	1720
BspMI	ACCTGC	1 cut(s)	1119
BspPI	GGATC	3 cut(s)	422, 435, 1462
BspT104I	TTCGAA	1 cut(s)	1047
BspT107I	GGYRCC	3 cut(s)	305, 919, 979
BsrI	ACTGG	2 cut(s)	830, 1219
BssECI	CCNNGG	4 cut(s)	375, 526, 972, 1562
BssMI	GATC	4 cut(s)	427, 1105, 1231, 1467
BssNI	GRCGYC	1 cut(s)	491
BssT1I	CCWWGG	1 cut(s)	526
Bst2UI	CCWGG	4 cut(s)	376, 973, 1564, 1608
Bst4CI	ACNGT	5 cut(s)	293, 461, 692, 764, 1454
BstACI	GRCGYC	1 cut(s)	491
BstBAI	YACGTR	1 cut(s)	1115
BstBI	TTCGAA	1 cut(s)	1047
BstC8I	GCNNGC	3 cut(s)	34, 509, 1713
BstDEI	CTNAG	5 cut(s)	15, 886, 1019, 1381, 1549
BstEII	GGTNACC	1 cut(s)	758
BstENI	CCTNNNNNAGG	1 cut(s)	525
BstF5I	GGATG	6 cut(s)	220, 532, 1051, 1259, 1588, 1752
BstFNI	CGCG	3 cut(s)	36, 244, 1387
BstH2I	RGCGCY	2 cut(s)	103, 236
BstHHI	GCGC	3 cut(s)	102, 235, 1387
BstKTI	GATC	4 cut(s)	430, 1108, 1234, 1470
BstMAI	GTCTC	2 cut(s)	57, 123
BstMBI	GATC	4 cut(s)	427, 1105, 1231, 1467
BstMWI	GCNNNNNNNGC	9 cut(s)	116, 241, 305, 338, 517, 896, 1118, 1433, 1592
BstNI	CCWGG	4 cut(s)	376, 973, 1564, 1608
BstNSI	RCATGY	2 cut(s)	332, 1715
BstPI	GGTNACC	1 cut(s)	758
BstSCI	CCNGG	6 cut(s)	160, 374, 971, 1562, 1606, 1671
BstSFI	CTRYAG	1 cut(s)	1716
BstSLI	GKGCMC	2 cut(s)	174, 611
BstUI	CGCG	3 cut(s)	36, 244, 1387
BstV1I	GCAGC	5 cut(s)	106, 529, 1290, 1582, 1702
BstV2I	GAAGAC	1 cut(s)	1549
BstX2I	RGATCY	2 cut(s)	427, 1105
BstYI	RGATCY	2 cut(s)	427, 1105
BsuRI	GGCC	1 cut(s)	81
BtsCI	GGATG	6 cut(s)	220, 532, 1051, 1259, 1588, 1752
BtsIMutI	CAGTG	2 cut(s)	160, 466
BveI	ACCTGC	1 cut(s)	1119
Cac8I	GCNNGC	3 cut(s)	34, 509, 1713
CfoI	GCGC	3 cut(s)	102, 235, 1387
Cfr13I	GGNCC	1 cut(s)	1159
CseI	GACGC	3 cut(s)	25, 1088, 1376
Csp6I	GTAC	5 cut(s)	711, 752, 920, 980, 1611
CviAII	CATG	8 cut(s)	284, 329, 755, 910, 916, 1223, 1680, 1712
CviQI	GTAC	5 cut(s)	711, 752, 920, 980, 1611
DdeI	CTNAG	5 cut(s)	15, 886, 1019, 1381, 1549
DpnI	GATC	4 cut(s)	429, 1107, 1233, 1469
DpnII	GATC	4 cut(s)	427, 1105, 1231, 1467
DraI	TTTAAA	2 cut(s)	624, 1687
EaeI	YGGCCR	1 cut(s)	79
EciI	GGCGGA	1 cut(s)	218
Eco130I	CCWWGG	1 cut(s)	526
Eco24I	GRGCYC	1 cut(s)	1563
Eco32I	GATATC	1 cut(s)	1396
Eco47I	GGWCC	1 cut(s)	1159
Eco47III	AGCGCT	1 cut(s)	234
Eco57I	CTGAAG	3 cut(s)	206, 876, 1635
Eco72I	CACGTG	1 cut(s)	1115
Eco91I	GGTNACC	1 cut(s)	758
EcoNI	CCTNNNNNAGG	1 cut(s)	525
EcoO109I	RGGNCCY	1 cut(s)	1159
EcoO65I	GGTNACC	1 cut(s)	758
EcoRI	GAATTC	1 cut(s)	1512
EcoRII	CCWGG	4 cut(s)	374, 971, 1562, 1606
EcoRV	GATATC	1 cut(s)	1396
EcoT14I	CCWWGG	1 cut(s)	526
EcoT22I	ATGCAT	2 cut(s)	881, 1713
EcoT38I	GRGCYC	1 cut(s)	1563
ErhI	CCWWGG	1 cut(s)	526
FaeI	CATG	8 cut(s)	287, 332, 758, 913, 919, 1226, 1683, 1715
FaqI	GGGAC	2 cut(s)	283, 1312
FatI	CATG	8 cut(s)	283, 328, 754, 909, 915, 1222, 1679, 1711
FauI	CCCGC	1 cut(s)	550
FblI	GTMKAC	1 cut(s)	1666
Fnu4HI	GCNGC	7 cut(s)	120, 245, 518, 1279, 1282, 1596, 1716
FokI	GGATG	6 cut(s)	227, 519, 1038, 1266, 1595, 1759
FriOI	GRGCYC	1 cut(s)	1563
Fsp4HI	GCNGC	7 cut(s)	120, 245, 518, 1279, 1282, 1596, 1716
FspBI	CTAG	3 cut(s)	797, 990, 1109
GlaI	GCGC	3 cut(s)	101, 234, 1386
GluI	GCNGC	7 cut(s)	120, 245, 518, 1279, 1282, 1596, 1716
GsuI	CTGGAG	1 cut(s)	99
HaeII	RGCGCY	2 cut(s)	103, 236
HaeIII	GGCC	1 cut(s)	81
HapII	CCGG	3 cut(s)	161, 900, 1673
HgaI	GACGC	3 cut(s)	25, 1088, 1376
HhaI	GCGC	3 cut(s)	102, 235, 1387
Hin1I	GRCGYC	1 cut(s)	491
Hin1II	CATG	8 cut(s)	287, 332, 758, 913, 919, 1226, 1683, 1715
Hin6I	GCGC	3 cut(s)	100, 233, 1385
HinP1I	GCGC	3 cut(s)	100, 233, 1385
HinfI	GANTC	5 cut(s)	253, 667, 968, 1528, 1668
HpaII	CCGG	3 cut(s)	161, 900, 1673
HphI	GGTGA	3 cut(s)	752, 1433, 1687
Hpy166II	GTNNAC	1 cut(s)	1667
Hpy188III	TCNNGA	6 cut(s)	368, 686, 784, 1297, 1391, 1539
Hpy8I	GTNNAC	1 cut(s)	1667
Hpy99I	CGWCG	1 cut(s)	244
HpyAV	CCTTC	7 cut(s)	339, 532, 596, 824, 851, 1433, 1610
HpyCH4III	ACNGT	5 cut(s)	293, 461, 692, 764, 1454
HpyCH4IV	ACGT	3 cut(s)	168, 491, 1114
HpyF10VI	GCNNNNNNNGC	9 cut(s)	116, 241, 305, 338, 517, 896, 1118, 1433, 1592
HpyF3I	CTNAG	5 cut(s)	15, 886, 1019, 1381, 1549
HpySE526I	ACGT	3 cut(s)	168, 491, 1114
Hsp92I	GRCGYC	1 cut(s)	491
Hsp92II	CATG	8 cut(s)	287, 332, 758, 913, 919, 1226, 1683, 1715
HspAI	GCGC	3 cut(s)	100, 233, 1385
KpnI	GGTACC	2 cut(s)	923, 983
Kzo9I	GATC	4 cut(s)	427, 1105, 1231, 1467
LmnI	GCTCC	3 cut(s)	163, 409, 1432
Lsp1109I	GCAGC	5 cut(s)	106, 529, 1290, 1582, 1702
LweI	GCATC	6 cut(s)	265, 434, 587, 991, 1573, 1624
MaeI	CTAG	3 cut(s)	797, 990, 1109
MaeII	ACGT	3 cut(s)	168, 491, 1114
MaeIII	GTNAC	5 cut(s)	455, 758, 1210, 1448, 1675
MalI	GATC	4 cut(s)	429, 1107, 1233, 1469
MboI	GATC	4 cut(s)	427, 1105, 1231, 1467
MboII	GAAGA	6 cut(s)	453, 476, 1159, 1169, 1349, 1554
MflI	RGATCY	2 cut(s)	427, 1105
MhlI	GDGCHC	4 cut(s)	160, 174, 611, 1563
MlsI	TGGCCA	1 cut(s)	81
MluCI	AATT	9 cut(s)	93, 474, 810, 834, 938, 950, 1350, 1512, 1626
MluNI	TGGCCA	1 cut(s)	81
MlyI	GAGTC	1 cut(s)	1662
MmeI	TCCRAC	2 cut(s)	577, 1312
Mox20I	TGGCCA	1 cut(s)	81
Mph1103I	ATGCAT	2 cut(s)	881, 1713
MroXI	GAANNNNTTC	1 cut(s)	1122
MscI	TGGCCA	1 cut(s)	81
MseI	TTAA	7 cut(s)	623, 804, 894, 945, 953, 1320, 1686
MslI	CAYNNNNRTG	2 cut(s)	914, 1227
Msp20I	TGGCCA	1 cut(s)	81
MspA1I	CMGCKG	2 cut(s)	119, 735
MspI	CCGG	3 cut(s)	161, 900, 1673
MspR9I	CCNGG	6 cut(s)	162, 376, 973, 1564, 1608, 1673
Mva1269I	GAATGC	1 cut(s)	640
MvaI	CCWGG	4 cut(s)	376, 973, 1564, 1608
MvnI	CGCG	3 cut(s)	36, 244, 1387
MwoI	GCNNNNNNNGC	9 cut(s)	116, 241, 305, 338, 517, 896, 1118, 1433, 1592
NciI	CCSGG	2 cut(s)	162, 1673
NdeII	GATC	4 cut(s)	427, 1105, 1231, 1467
NlaIII	CATG	8 cut(s)	287, 332, 758, 913, 919, 1226, 1683, 1715
NlaIV	GGNNCC	5 cut(s)	307, 429, 921, 981, 1161
NmuCI	GTSAC	2 cut(s)	758, 1675
NsiI	ATGCAT	2 cut(s)	881, 1713
NspI	RCATGY	2 cut(s)	332, 1715
NspV	TTCGAA	1 cut(s)	1047
PaeI	GCATGC	1 cut(s)	1715
PctI	GAATGC	1 cut(s)	640
PdmI	GAANNNNTTC	1 cut(s)	1122
PfeI	GAWTC	4 cut(s)	253, 667, 968, 1528
PflFI	GACNNNGTC	1 cut(s)	911
PkrI	GCNGC	7 cut(s)	121, 246, 519, 1280, 1283, 1597, 1717
PleI	GAGTC	1 cut(s)	1662
PmaCI	CACGTG	1 cut(s)	1115
PmlI	CACGTG	1 cut(s)	1115
PpsI	GAGTC	1 cut(s)	1662
Ppu21I	YACGTR	1 cut(s)	1115
PpuMI	RGGWCCY	1 cut(s)	1159
PshBI	ATTAAT	2 cut(s)	894, 953
Psp5II	RGGWCCY	1 cut(s)	1159
Psp6I	CCWGG	4 cut(s)	374, 971, 1562, 1606
PspCI	CACGTG	1 cut(s)	1115
PspEI	GGTNACC	1 cut(s)	758
PspGI	CCWGG	4 cut(s)	374, 971, 1562, 1606
PspN4I	GGNNCC	5 cut(s)	307, 429, 921, 981, 1161
PspPI	GGNCC	1 cut(s)	1159
PspPPI	RGGWCCY	1 cut(s)	1159
PstI	CTGCAG	1 cut(s)	1720
PsuI	RGATCY	2 cut(s)	427, 1105
PsyI	GACNNNGTC	1 cut(s)	911
PvuII	CAGCTG	2 cut(s)	119, 735
RsaI	GTAC	5 cut(s)	712, 753, 921, 981, 1612
RsaNI	GTAC	5 cut(s)	711, 752, 920, 980, 1611
RseI	CAYNNNNRTG	2 cut(s)	914, 1227
SaqAI	TTAA	7 cut(s)	623, 804, 894, 945, 953, 1320, 1686
SatI	GCNGC	7 cut(s)	120, 245, 518, 1279, 1282, 1596, 1716
Sau3AI	GATC	4 cut(s)	427, 1105, 1231, 1467
Sau96I	GGNCC	1 cut(s)	1159
SchI	GAGTC	1 cut(s)	1662
ScrFI	CCNGG	6 cut(s)	162, 376, 973, 1564, 1608, 1673
SduI	GDGCHC	4 cut(s)	160, 174, 611, 1563
SfaNI	GCATC	6 cut(s)	265, 434, 587, 991, 1573, 1624
SfcI	CTRYAG	1 cut(s)	1716
SfuI	TTCGAA	1 cut(s)	1047
SinI	GGWCC	1 cut(s)	1159
SmiMI	CAYNNNNRTG	2 cut(s)	914, 1227
SmlI	CTYRAG	1 cut(s)	699
SmoI	CTYRAG	1 cut(s)	699
SphI	GCATGC	1 cut(s)	1715
Sse9I	AATT	9 cut(s)	93, 474, 810, 834, 938, 950, 1350, 1512, 1626
SsiI	CCGC	5 cut(s)	203, 244, 278, 557, 1282
SspI	AATATT	1 cut(s)	1364
SspMI	CTAG	3 cut(s)	797, 990, 1109
StyD4I	CCNGG	6 cut(s)	160, 374, 971, 1562, 1606, 1671
StyI	CCWWGG	1 cut(s)	526
TaaI	ACNGT	5 cut(s)	293, 461, 692, 764, 1454
TaiI	ACGT	3 cut(s)	171, 494, 1117
TaqI	TCGA	4 cut(s)	343, 1047, 1499, 1659
TasI	AATT	9 cut(s)	93, 474, 810, 834, 938, 950, 1350, 1512, 1626
TatI	WGTACW	2 cut(s)	710, 751
TauI	GCSGC	2 cut(s)	247, 1284
TfiI	GAWTC	4 cut(s)	253, 667, 968, 1528
Tru1I	TTAA	7 cut(s)	623, 804, 894, 945, 953, 1320, 1686
Tru9I	TTAA	7 cut(s)	623, 804, 894, 945, 953, 1320, 1686
TscAI	CASTG	2 cut(s)	160, 466
TseFI	GTSAC	2 cut(s)	758, 1675
TseI	GCWGC	5 cut(s)	119, 517, 1278, 1595, 1715
Tsp45I	GTSAC	2 cut(s)	758, 1675
TspDTI	ATGAA	5 cut(s)	272, 659, 931, 948, 1181
TspGWI	ACGGA	3 cut(s)	396, 1167, 1419
TspRI	CASTG	2 cut(s)	160, 466
Tth111I	GACNNNGTC	1 cut(s)	911
VpaK11BI	GGWCC	1 cut(s)	1159
VspI	ATTAAT	2 cut(s)	894, 953
XagI	CCTNNNNNAGG	1 cut(s)	525
XapI	RAATTY	3 cut(s)	810, 938, 1512
XceI	RCATGY	2 cut(s)	332, 1715
XmiI	GTMKAC	1 cut(s)	1666
XmnI	GAANNNNTTC	1 cut(s)	1122
XspI	CTAG	3 cut(s)	797, 990, 1109
ZraI	GACGTC	1 cut(s)	492
Zsp2I	ATGCAT	2 cut(s)	881, 1713

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.