Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001232

Physical Location & Seq

Genomic Coordinates Forward (+)

29235421 .. 29242710

Gene Structure 7290 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1242100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1788 bp

ATGGCGAAAGTAGCAGAGGAACACGTTGAAGCTCGCGTCAAACAACTCCTGTCTCGTATGACTTTGAAAGACAAGGTTGGCCAGATGACCCAAATCGAGCGCCAGGTCGCCACTCCCGCTGCCGTTAGAGACCACTCCATAGGGAGTATATTCAGTGCCCCGGGCAACGTGCCCTGTGAAAAGGCTTCGCCTTCAGAATGGGCGGATATGGTGGATGGGCTTCAGAGGTCAGCACTAACGTCGCGGCTTGGGATTCCTCTCATATATGCGACTGATGCGGTTCATGGCAACGGTAATGTTTATGGTGCTACTATATTCCCTCACAACGTTGGTGTTGGCGCCACTAGAGATGCGGATTTGGCTCAAAGGATTGGTGTGGCAACTGCTCTTGAAGCCAGGGCTTGTGGTTTTCACTATATAATTGCTCCCTGTGTGGCTGTATGCAAAGATCCGAGATGGGGAAGATGCTACGAGAGTTACAGTGAAGACACTGAAATTGTAAGCAAGATGACGTCTATCGTTTCAGGCTTACAGGGGCAGCCACCCCAAGGACATCCCGAGGGCTACCCTTTTATAGCGGGAAGCAACAACACTATTGCATGTGCCATGCATTTTGTTGGAGAAGGGGGTACAGACAAAGGTGTAAATGAGGGTAATGCTGTATTATCTTACGAGGATTTAGAGAGGATTCATATGAAACCTTATCTGGACTGTATTTCTCAAGGTGTTTGTACTATTATGGCTTCCTATAACAGCTGGAATGGAGATAGAGTACATGGTCACCGTTTTCTCCTTACAGAAATCTTGAAAGATAAGCTAGGTTTTAAGGGAATTTTGATTTCTGACTGGGAAGGAATTGACCAACTTTGCGAACCTGAAGGTTCAGACTATCGTTTATGCATTTCCTTAGCCATTAATGCAGGAATAGACATGGTCATGGTGCCTTTCAGATATGAAAAATTCATCGAGGAATTAATATATCTTGTGGAATCCAGGGAGGTACCAATGTCAAGGATAGATGACGCTGTTGCTAGAATACTGAGAGTGAAGTTTGTTGCTGGTGTTTTCGAACATCCCTTCGCTGATAAATCTTTACAGGACGCAGTTGGTTGCAAGTTGCACAGAGATCTAGCACGTGAAGCAGTTCGCAGGTCATTGGTTCTGTTGAAGAACGGAAAGGACCCAATGAAACCTTTTCTTCCATTAGACAGAAATGCTAAAAGAATACTTGTTGCTGGAACTCATGCCGATGATCTTGGAAATCAATGTGGAGGATGGACCGCTACAAAATATGGTAGCAGCGGCCAGATAACAATCGGGACAACCATCTTGGAAGCTATTAAGATGGCAGTTGGAGATGATACAGAAATAATTTACGAGAAATATCCATCAGCAGACACCTTAGCGCATCAAGGTATCTCTTTTGCGATTGTGGTTGTTGGTGAAGCTCCGTATGCAGAAGGCAGGGGTTACAGTTCAGAGCTTGTGATCCCGTTCAATGGAACTGATGTCATAAGCTCGATTGCTGACAGAATTCCCGTCTTGGCGATTCTGATTTCTGGAAGACCCTTAGTTTTAGAGCCTTCGCTCTTGGAAAAGATGGATGCTCTTGTTGCTGCTTGGCTCCCTGGTACTGAAGGAGAGGGAATTGTAGATGCAATCTTTGGAGATTTTGACTTCGAGGGTAGACTCCCGGTGACATGGTTTAAAATGGTTGAACAACTGCCCATGCATGCTGCAGACCCTTTATATCCTCTTGGCTTTGGGATGACGTACAATAATGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005886 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0015926 GO:0016020 GO:0016052 GO:0016787 GO:0016798 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:0071944 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

596

Amino Acids

64.85

Weight (kDa)

5.25

Isoelectric Point (pI)

33.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	21 - 349	8.4e-70	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	386 - 592	8.8e-35	Glycosyl hydrolase family 3 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001232)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G47000 AT3G47000 AT3G47000 AT3G47010 AT3G47010 AT3G47010 AT3G47040 AT3G47040 AT3G47040 AT3G47050
fragaria_vesca	FvH4_3g38160 FvH4_3g38160 FvH4_4g11940
malus_domestica	MD02G1242000.v1.1 MD02G1242100.v1.1 MD02G1242200.v1.1 MD02G1242300.v1.1 MD11G1080700.v1.1
prunus_persica	Prupe.2G092300_v2.0.a1 Prupe.2G092300_v2.0.a1 Prupe.2G092300_v2.0.a1 Prupe.2G092600_v2.0.a1 Prupe.2G092900_v2.0.a1 Prupe.2G092900_v2.0.a1 Prupe.6G061000_v2.0.a1 Prupe.6G061000_v2.0.a1 Prupe.6G061000_v2.0.a1 Prupe.6G061000_v2.0.a1 Prupe.6G061000_v2.0.a1
pyrus_communis	pycom02g20900 pycom11g06800 pycom11g06860
rosa_chinensis	RchiOBHm_Chr5g0068591 RchiOBHm_Chr6g0246121
rosa_laevigata	RLG00000035983 RLG00000036820 RLG00000036839
rosa_multiflora	Rmu_sc0004721.1_g000003
rosa_roxburghii	Rroxscaffold_1G00003560 Rroxscaffold_1G00012260 Rroxscaffold_3G00247860 Rroxscaffold_7G00214870
rosa_rugosa	Rorug05G0391700 Rorug05G0464600 Rorug05G0465200 Rorug05G0465800
rosa_samantha	Rh5AG449400 Rh5BG467600 Rh5DG481000 Rh5DG551200 Rh5DG552100 Rh7AG258000 Rh7CG274700 Rh7DG265200
rosa_wichuraiana	Rw5G041960 Rw5G048090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	515
Acc36I	ACCTGC	1 cut(s)	1140
Acc65I	GGTACC	1 cut(s)	1000
AccB1I	GGYRCC	3 cut(s)	338, 940, 1000
AccI	GTMKAC	1 cut(s)	1687
AccII	CGCG	2 cut(s)	36, 244
AciI	CCGC	8 cut(s)	117, 203, 244, 278, 353, 578, 1281, 1302
AclI	AACGTT	1 cut(s)	327
AclWI	GGATC	2 cut(s)	443, 1483
AcoI	YGGCCR	2 cut(s)	79, 1303
AcsI	RAATTY	3 cut(s)	831, 959, 1533
AcuI	CTGAAG	4 cut(s)	177, 206, 897, 1656
AcvI	CACGTG	1 cut(s)	1136
AcyI	GRCGYC	2 cut(s)	339, 512
AfaI	GTAC	6 cut(s)	631, 733, 774, 1002, 1633, 1775
AfiI	CCNNNNNNNGG	3 cut(s)	458, 548, 1499
AflIII	ACRYGT	1 cut(s)	22
AgsI	TTSAA	7 cut(s)	29, 67, 392, 808, 1168, 1498, 1718
AjnI	CCWGG	4 cut(s)	102, 395, 992, 1627
AluBI	AGCT	7 cut(s)	32, 756, 817, 1337, 1448, 1483, 1518
AluI	AGCT	7 cut(s)	32, 756, 817, 1337, 1448, 1483, 1518
Alw26I	GTCTC	2 cut(s)	57, 123
AlwI	GGATC	2 cut(s)	443, 1483
Ama87I	CYCGRG	2 cut(s)	160, 557
AoxI	GGCC	2 cut(s)	79, 1303
ApeKI	GCWGC	5 cut(s)	119, 538, 1299, 1616, 1736
ApoI	RAATTY	3 cut(s)	831, 959, 1533
AseI	ATTAAT	2 cut(s)	915, 974
Asp700I	GAANNNNTTC	1 cut(s)	1143
Asp718I	GGTACC	1 cut(s)	1000
AspLEI	GCGC	3 cut(s)	102, 341, 1408
AspS9I	GGNCC	2 cut(s)	1180, 1278
AsuC2I	CCSGG	3 cut(s)	161, 162, 1694
AsuHPI	GGTGA	3 cut(s)	773, 1454, 1708
AsuII	TTCGAA	1 cut(s)	1068
AvaI	CYCGRG	2 cut(s)	160, 557
AvaII	GGWCC	2 cut(s)	1180, 1278
BaeGI	GKGCMC	2 cut(s)	160, 174
BaeI	ACNNNNGTAYC	2 cut(s)	992, 1025
BalI	TGGCCA	1 cut(s)	81
BanI	GGYRCC	3 cut(s)	338, 940, 1000
BbrPI	CACGTG	1 cut(s)	1136
BbsI	GAAGAC	2 cut(s)	492, 1570
BbvI	GCAGC	5 cut(s)	106, 550, 1311, 1603, 1723
BccI	CCATC	7 cut(s)	209, 450, 1269, 1334, 1339, 1396, 1594
BceAI	ACGGC	1 cut(s)	107
BcgI	CGANNNNNNTGC	2 cut(s)	222, 256
BciT130I	CCWGG	4 cut(s)	104, 397, 994, 1629
BcnI	CCSGG	3 cut(s)	161, 162, 1694
BcoDI	GTCTC	2 cut(s)	57, 123
BfaI	CTAG	4 cut(s)	345, 818, 1032, 1130
BfmI	CTRYAG	1 cut(s)	1737
BfoI	RGCGCY	2 cut(s)	103, 342
BfuAI	ACCTGC	1 cut(s)	1140
BglII	AGATCT	1 cut(s)	1126
BisI	GCNGC	7 cut(s)	120, 245, 539, 1300, 1303, 1617, 1737
BlsI	GCNGC	7 cut(s)	121, 246, 540, 1301, 1304, 1618, 1738
Bme1390I	CCNGG	7 cut(s)	104, 161, 162, 397, 994, 1629, 1694
Bme18I	GGWCC	2 cut(s)	1180, 1278
BmeT110I	CYCGRG	2 cut(s)	160, 557
BmgT120I	GGNCC	2 cut(s)	1180, 1278
BmiI	GGNNCC	5 cut(s)	340, 942, 1002, 1182, 1625
BmrFI	CCNGG	7 cut(s)	104, 161, 162, 397, 994, 1629, 1694
BmrI	ACTGGG	1 cut(s)	856
BmsI	GCATC	6 cut(s)	265, 340, 455, 1417, 1594, 1645
BmuI	ACTGGG	1 cut(s)	856
BpiI	GAAGAC	2 cut(s)	492, 1570
Bpu10I	CCTNAGC	2 cut(s)	907, 1402
Bpu14I	TTCGAA	1 cut(s)	1068
BpuEI	CTTGAG	1 cut(s)	705
BpuMI	CCSGG	3 cut(s)	161, 162, 1694
BsaAI	YACGTR	1 cut(s)	1136
BsaBI	GATNNNNATC	1 cut(s)	1313
BsaHI	GRCGYC	2 cut(s)	339, 512
BsaI	GGTCTC	1 cut(s)	123
BsaJI	CCNNGG	7 cut(s)	159, 160, 396, 547, 558, 993, 1627
BsaXI	ACNNNNNCTCC	2 cut(s)	756, 786
Bsc4I	CCNNNNNNNGG	3 cut(s)	458, 548, 1499
Bse1I	ACTGG	1 cut(s)	851
Bse8I	GATNNNNATC	1 cut(s)	1313
BseBI	CCWGG	4 cut(s)	104, 397, 994, 1629
BseDI	CCNNGG	7 cut(s)	159, 160, 396, 547, 558, 993, 1627
BseGI	GGATG	6 cut(s)	220, 553, 1072, 1280, 1609, 1773
BseJI	GATNNNNATC	1 cut(s)	1313
BseLI	CCNNNNNNNGG	3 cut(s)	458, 548, 1499
BseMII	CTCAG	1 cut(s)	1031
BseNI	ACTGG	1 cut(s)	851
BseSI	GKGCMC	2 cut(s)	160, 174
BseXI	GCAGC	5 cut(s)	106, 550, 1311, 1603, 1723
Bsh1236I	CGCG	2 cut(s)	36, 244
BshFI	GGCC	2 cut(s)	81, 1305
BshNI	GGYRCC	3 cut(s)	338, 940, 1000
BsiHKCI	CYCGRG	2 cut(s)	160, 557
BsiSI	CCGG	2 cut(s)	161, 1694
BslFI	GGGAC	1 cut(s)	1333
BslI	CCNNNNNNNGG	3 cut(s)	458, 548, 1499
BsmAI	GTCTC	2 cut(s)	57, 123
BsmFI	GGGAC	1 cut(s)	1333
BsnI	GGCC	2 cut(s)	81, 1305
Bso31I	GGTCTC	1 cut(s)	123
BsoBI	CYCGRG	2 cut(s)	160, 557
Bsp119I	TTCGAA	1 cut(s)	1068
Bsp1286I	GDGCHC	2 cut(s)	160, 174
Bsp143I	GATC	4 cut(s)	448, 1126, 1252, 1488
BspACI	CCGC	8 cut(s)	117, 203, 244, 278, 353, 578, 1281, 1302
BspANI	GGCC	2 cut(s)	81, 1305
BspCNI	CTCAG	1 cut(s)	1032
BspFNI	CGCG	2 cut(s)	36, 244
BspLI	GGNNCC	5 cut(s)	340, 942, 1002, 1182, 1625
BspMAI	CTGCAG	1 cut(s)	1741
BspMI	ACCTGC	1 cut(s)	1140
BspPI	GGATC	2 cut(s)	443, 1483
BspT104I	TTCGAA	1 cut(s)	1068
BspT107I	GGYRCC	3 cut(s)	338, 940, 1000
BspTNI	GGTCTC	1 cut(s)	123
BsrI	ACTGG	1 cut(s)	851
BssECI	CCNNGG	7 cut(s)	159, 160, 396, 547, 558, 993, 1627
BssMI	GATC	4 cut(s)	448, 1126, 1252, 1488
BssNI	GRCGYC	2 cut(s)	339, 512
BssT1I	CCWWGG	1 cut(s)	547
Bst2UI	CCWGG	4 cut(s)	104, 397, 994, 1629
Bst4CI	ACNGT	5 cut(s)	293, 482, 713, 785, 1475
BstACI	GRCGYC	2 cut(s)	339, 512
BstBAI	YACGTR	1 cut(s)	1136
BstBI	TTCGAA	1 cut(s)	1068
BstC8I	GCNNGC	2 cut(s)	34, 1734
BstDEI	CTNAG	4 cut(s)	907, 1040, 1402, 1570
BstEII	GGTNACC	1 cut(s)	779
BstF5I	GGATG	6 cut(s)	220, 553, 1072, 1280, 1609, 1773
BstFNI	CGCG	2 cut(s)	36, 244
BstH2I	RGCGCY	2 cut(s)	103, 342
BstHHI	GCGC	3 cut(s)	102, 341, 1408
BstKTI	GATC	4 cut(s)	451, 1129, 1255, 1491
BstMAI	GTCTC	2 cut(s)	57, 123
BstMBI	GATC	4 cut(s)	448, 1126, 1252, 1488
BstMWI	GCNNNNNNNGC	9 cut(s)	11, 116, 275, 359, 392, 917, 1139, 1454, 1613
BstNI	CCWGG	4 cut(s)	104, 397, 994, 1629
BstNSI	RCATGY	2 cut(s)	603, 1736
BstPI	GGTNACC	1 cut(s)	779
BstSCI	CCNGG	7 cut(s)	102, 159, 160, 395, 992, 1627, 1692
BstSFI	CTRYAG	1 cut(s)	1737
BstSLI	GKGCMC	2 cut(s)	160, 174
BstUI	CGCG	2 cut(s)	36, 244
BstV1I	GCAGC	5 cut(s)	106, 550, 1311, 1603, 1723
BstV2I	GAAGAC	2 cut(s)	492, 1570
BstX2I	RGATCY	2 cut(s)	448, 1126
BstYI	RGATCY	2 cut(s)	448, 1126
BsuRI	GGCC	2 cut(s)	81, 1305
BtsCI	GGATG	6 cut(s)	220, 553, 1072, 1280, 1609, 1773
BtsIMutI	CAGTG	3 cut(s)	160, 487, 489
BveI	ACCTGC	1 cut(s)	1140
Cac8I	GCNNGC	2 cut(s)	34, 1734
CfoI	GCGC	3 cut(s)	102, 341, 1408
Cfr13I	GGNCC	2 cut(s)	1180, 1278
Cfr9I	CCCGGG	1 cut(s)	160
CseI	GACGC	3 cut(s)	25, 1031, 1109
Csp6I	GTAC	6 cut(s)	630, 732, 773, 1001, 1632, 1774
CviQI	GTAC	6 cut(s)	630, 732, 773, 1001, 1632, 1774
DdeI	CTNAG	4 cut(s)	907, 1040, 1402, 1570
DinI	GGCGCC	1 cut(s)	340
DpnI	GATC	4 cut(s)	450, 1128, 1254, 1490
DpnII	GATC	4 cut(s)	448, 1126, 1252, 1488
DraI	TTTAAA	1 cut(s)	1708
EaeI	YGGCCR	2 cut(s)	79, 1303
EciI	GGCGGA	1 cut(s)	218
Eco130I	CCWWGG	1 cut(s)	547
Eco31I	GGTCTC	1 cut(s)	123
Eco47I	GGWCC	2 cut(s)	1180, 1278
Eco57I	CTGAAG	4 cut(s)	177, 206, 897, 1656
Eco72I	CACGTG	1 cut(s)	1136
Eco88I	CYCGRG	2 cut(s)	160, 557
Eco91I	GGTNACC	1 cut(s)	779
EcoO109I	RGGNCCY	1 cut(s)	1180
EcoO65I	GGTNACC	1 cut(s)	779
EcoRI	GAATTC	1 cut(s)	1533
EcoRII	CCWGG	4 cut(s)	102, 395, 992, 1627
EcoT14I	CCWWGG	1 cut(s)	547
EcoT22I	ATGCAT	3 cut(s)	612, 902, 1734
EgeI	GGCGCC	1 cut(s)	340
EheI	GGCGCC	1 cut(s)	340
ErhI	CCWWGG	1 cut(s)	547
FaqI	GGGAC	1 cut(s)	1333
FauI	CCCGC	2 cut(s)	124, 571
FauNDI	CATATG	1 cut(s)	693
FblI	GTMKAC	1 cut(s)	1687
Fnu4HI	GCNGC	7 cut(s)	120, 245, 539, 1300, 1303, 1617, 1737
FokI	GGATG	6 cut(s)	227, 540, 1059, 1287, 1616, 1780
Fsp4HI	GCNGC	7 cut(s)	120, 245, 539, 1300, 1303, 1617, 1737
FspBI	CTAG	4 cut(s)	345, 818, 1032, 1130
GlaI	GCGC	3 cut(s)	101, 340, 1407
GluI	GCNGC	7 cut(s)	120, 245, 539, 1300, 1303, 1617, 1737
HaeII	RGCGCY	2 cut(s)	103, 342
HaeIII	GGCC	2 cut(s)	81, 1305
HapII	CCGG	2 cut(s)	161, 1694
HgaI	GACGC	3 cut(s)	25, 1031, 1109
HhaI	GCGC	3 cut(s)	102, 341, 1408
Hin1I	GRCGYC	2 cut(s)	339, 512
Hin6I	GCGC	3 cut(s)	100, 339, 1406
HinP1I	GCGC	3 cut(s)	100, 339, 1406
HinfI	GANTC	5 cut(s)	253, 688, 989, 1549, 1689
HpaII	CCGG	2 cut(s)	161, 1694
HphI	GGTGA	3 cut(s)	773, 1454, 1708
Hpy166II	GTNNAC	1 cut(s)	1688
Hpy188I	TCNGA	8 cut(s)	196, 225, 453, 844, 886, 950, 1480, 1554
Hpy188III	TCNNGA	6 cut(s)	389, 557, 707, 805, 1318, 1560
Hpy8I	GTNNAC	1 cut(s)	1688
Hpy99I	CGWCG	1 cut(s)	244
HpyAV	CCTTC	8 cut(s)	201, 617, 845, 872, 1087, 1454, 1593, 1631
HpyCH4III	ACNGT	5 cut(s)	293, 482, 713, 785, 1475
HpyCH4IV	ACGT	7 cut(s)	24, 168, 239, 327, 512, 1135, 1772
HpyF10VI	GCNNNNNNNGC	9 cut(s)	11, 116, 275, 359, 392, 917, 1139, 1454, 1613
HpyF3I	CTNAG	4 cut(s)	907, 1040, 1402, 1570
HpySE526I	ACGT	7 cut(s)	24, 168, 239, 327, 512, 1135, 1772
Hsp92I	GRCGYC	2 cut(s)	339, 512
HspAI	GCGC	3 cut(s)	100, 339, 1406
KasI	GGCGCC	1 cut(s)	338
KpnI	GGTACC	1 cut(s)	1004
Kzo9I	GATC	4 cut(s)	448, 1126, 1252, 1488
LmnI	GCTCC	3 cut(s)	430, 1453, 1629
Lsp1109I	GCAGC	5 cut(s)	106, 550, 1311, 1603, 1723
LweI	GCATC	6 cut(s)	265, 340, 455, 1417, 1594, 1645
MaeI	CTAG	4 cut(s)	345, 818, 1032, 1130
MaeII	ACGT	7 cut(s)	24, 168, 239, 327, 512, 1135, 1772
MaeIII	GTNAC	4 cut(s)	476, 779, 1469, 1696
MalI	GATC	4 cut(s)	450, 1128, 1254, 1490
MboI	GATC	4 cut(s)	448, 1126, 1252, 1488
MboII	GAAGA	5 cut(s)	474, 497, 1180, 1190, 1575
MflI	RGATCY	2 cut(s)	448, 1126
MhlI	GDGCHC	2 cut(s)	160, 174
MlsI	TGGCCA	1 cut(s)	81
MluCI	AATT	9 cut(s)	420, 495, 831, 855, 959, 971, 1371, 1533, 1647
MluNI	TGGCCA	1 cut(s)	81
Mly113I	GGCGCC	1 cut(s)	339
MlyI	GAGTC	1 cut(s)	1683
MmeI	TCCRAC	2 cut(s)	598, 1333
Mox20I	TGGCCA	1 cut(s)	81
Mph1103I	ATGCAT	3 cut(s)	612, 902, 1734
MroXI	GAANNNNTTC	1 cut(s)	1143
MscI	TGGCCA	1 cut(s)	81
MseI	TTAA	5 cut(s)	825, 915, 974, 1341, 1707
MslI	CAYNNNNRTG	2 cut(s)	935, 1248
Msp20I	TGGCCA	1 cut(s)	81
MspA1I	CMGCKG	3 cut(s)	119, 756, 1302
MspI	CCGG	2 cut(s)	161, 1694
MspR9I	CCNGG	7 cut(s)	104, 161, 162, 397, 994, 1629, 1694
MvaI	CCWGG	4 cut(s)	104, 397, 994, 1629
MvnI	CGCG	2 cut(s)	36, 244
MwoI	GCNNNNNNNGC	9 cut(s)	11, 116, 275, 359, 392, 917, 1139, 1454, 1613
NarI	GGCGCC	1 cut(s)	339
NciI	CCSGG	3 cut(s)	161, 162, 1694
NdeI	CATATG	1 cut(s)	693
NdeII	GATC	4 cut(s)	448, 1126, 1252, 1488
NlaIV	GGNNCC	5 cut(s)	340, 942, 1002, 1182, 1625
NmuCI	GTSAC	2 cut(s)	779, 1696
NsiI	ATGCAT	3 cut(s)	612, 902, 1734
NspI	RCATGY	2 cut(s)	603, 1736
NspV	TTCGAA	1 cut(s)	1068
PaeI	GCATGC	1 cut(s)	1736
PdmI	GAANNNNTTC	1 cut(s)	1143
PfeI	GAWTC	4 cut(s)	253, 688, 989, 1549
PflFI	GACNNNGTC	1 cut(s)	932
PkrI	GCNGC	7 cut(s)	121, 246, 540, 1301, 1304, 1618, 1738
PleI	GAGTC	1 cut(s)	1683
PluTI	GGCGCC	1 cut(s)	342
PmaCI	CACGTG	1 cut(s)	1136
PmlI	CACGTG	1 cut(s)	1136
PpsI	GAGTC	1 cut(s)	1683
Ppu21I	YACGTR	1 cut(s)	1136
PpuMI	RGGWCCY	1 cut(s)	1180
PshBI	ATTAAT	2 cut(s)	915, 974
Psp1406I	AACGTT	1 cut(s)	327
Psp5II	RGGWCCY	1 cut(s)	1180
Psp6I	CCWGG	4 cut(s)	102, 395, 992, 1627
PspCI	CACGTG	1 cut(s)	1136
PspEI	GGTNACC	1 cut(s)	779
PspGI	CCWGG	4 cut(s)	102, 395, 992, 1627
PspN4I	GGNNCC	5 cut(s)	340, 942, 1002, 1182, 1625
PspPI	GGNCC	2 cut(s)	1180, 1278
PspPPI	RGGWCCY	1 cut(s)	1180
PstI	CTGCAG	1 cut(s)	1741
PsuI	RGATCY	2 cut(s)	448, 1126
PsyI	GACNNNGTC	1 cut(s)	932
PvuII	CAGCTG	1 cut(s)	756
RsaI	GTAC	6 cut(s)	631, 733, 774, 1002, 1633, 1775
RsaNI	GTAC	6 cut(s)	630, 732, 773, 1001, 1632, 1774
RseI	CAYNNNNRTG	2 cut(s)	935, 1248
SaqAI	TTAA	5 cut(s)	825, 915, 974, 1341, 1707
SatI	GCNGC	7 cut(s)	120, 245, 539, 1300, 1303, 1617, 1737
Sau3AI	GATC	4 cut(s)	448, 1126, 1252, 1488
Sau96I	GGNCC	2 cut(s)	1180, 1278
SchI	GAGTC	1 cut(s)	1683
ScrFI	CCNGG	7 cut(s)	104, 161, 162, 397, 994, 1629, 1694
SduI	GDGCHC	2 cut(s)	160, 174
SfaNI	GCATC	6 cut(s)	265, 340, 455, 1417, 1594, 1645
SfcI	CTRYAG	1 cut(s)	1737
SfoI	GGCGCC	1 cut(s)	340
SfuI	TTCGAA	1 cut(s)	1068
SinI	GGWCC	2 cut(s)	1180, 1278
SmaI	CCCGGG	1 cut(s)	162
SmiMI	CAYNNNNRTG	2 cut(s)	935, 1248
SmlI	CTYRAG	1 cut(s)	720
SmoI	CTYRAG	1 cut(s)	720
SphI	GCATGC	1 cut(s)	1736
Sse9I	AATT	9 cut(s)	420, 495, 831, 855, 959, 971, 1371, 1533, 1647
SsiI	CCGC	8 cut(s)	117, 203, 244, 278, 353, 578, 1281, 1302
SspDI	GGCGCC	1 cut(s)	338
SspMI	CTAG	4 cut(s)	345, 818, 1032, 1130
StyD4I	CCNGG	7 cut(s)	102, 159, 160, 395, 992, 1627, 1692
StyI	CCWWGG	1 cut(s)	547
TaaI	ACNGT	5 cut(s)	293, 482, 713, 785, 1475
TaiI	ACGT	7 cut(s)	27, 171, 242, 330, 515, 1138, 1775
TaqI	TCGA	5 cut(s)	96, 966, 1068, 1520, 1680
TasI	AATT	9 cut(s)	420, 495, 831, 855, 959, 971, 1371, 1533, 1647
TatI	WGTACW	2 cut(s)	731, 772
TauI	GCSGC	2 cut(s)	247, 1305
TfiI	GAWTC	4 cut(s)	253, 688, 989, 1549
Tru1I	TTAA	5 cut(s)	825, 915, 974, 1341, 1707
Tru9I	TTAA	5 cut(s)	825, 915, 974, 1341, 1707
TscAI	CASTG	3 cut(s)	160, 487, 496
TseFI	GTSAC	2 cut(s)	779, 1696
TseI	GCWGC	5 cut(s)	119, 538, 1299, 1616, 1736
Tsp45I	GTSAC	2 cut(s)	779, 1696
TspDTI	ATGAA	6 cut(s)	272, 680, 710, 952, 969, 1202
TspGWI	ACGGA	2 cut(s)	1188, 1440
TspMI	CCCGGG	1 cut(s)	160
TspRI	CASTG	3 cut(s)	160, 487, 496
Tth111I	GACNNNGTC	1 cut(s)	932
VpaK11BI	GGWCC	2 cut(s)	1180, 1278
VspI	ATTAAT	2 cut(s)	915, 974
XapI	RAATTY	3 cut(s)	831, 959, 1533
XceI	RCATGY	2 cut(s)	603, 1736
XmaI	CCCGGG	1 cut(s)	160
XmiI	GTMKAC	1 cut(s)	1687
XmnI	GAANNNNTTC	1 cut(s)	1143
XspI	CTAG	4 cut(s)	345, 818, 1032, 1130
ZraI	GACGTC	1 cut(s)	513
Zsp2I	ATGCAT	3 cut(s)	612, 902, 1734

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.