MD02G1301200.v1.1

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002998
Physical Location & Seq
Forward (+)
35583481 .. 35587913
4433 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1301200.v1.1.491

Sequence Viewer

Length: 1431 bp
ATGTTCAACGACAACAGCATATCAATCTCAGTCTCCGACGACGAAGCGGACGAGCTCGGCCGGATGAGAGTCCGAGTTCGAAGAAAGCGCAAGAAACTCGGCCAGCGACTCAAGCACCGAATCGCTAGAAAGCTCGTCAGGTACTGGGCGCTTCTCATTTTTATTCCGGCCCTCGGGTTGTTTCTGTTCGAGGCTTCCAGGATCGGCAAGAAGCCGAGTTTGGTTGAGTCGGAACGCAATACGGTGAAGAAGCCGAGTTTGGTTGAGTCGGAACGTAATACGGTGAAGAAGCCGAGTTTGGAGAAGAACTCGGATGCGAATTTGAATCGGCTTGATCATACGACTCGGGTTGTTGGAGGCGTTAGACAACGTTGTTTGGACATTCTATCTCCTGAAGAACTTGAGCAACTCGAAATTCCTGTGCGTGAAGAGTCCGATAGTCCTGTTAAGAAAGTATTATACAGATCAGAGAATGACACTTCGTTTTTAGGAGGGAACAACAGTCTTTCTCAAGAGCAACCAGAGGCCACAAGATTTAATTCATTTACTGGAACTCAAACACTAGATCAGAGAGAGAAATCGTTTAAGGTAAATGAAACTGTTACTATGAACTGTGGTTTCTACAGTGAAAATGGAGGGTTTAAAATCTCAAATGAAGACAAGGAGTACATGAATAGTTGCAAGGTTGTAGTGTCCACATGTGCATTTGGTGGTGGAGATGATCTTTATCAGCCTATTGGAATGTCAGAGGCATCACTTCGAAAGGTTTGCTATGTTGCTTTTTGGGATGATATTACTCTAAGTACACAGGAAGCGGCTGAACATAGAATTGATGGGGATGGATTTGTTGGGAAATGGCGCGTTGTGGTTGTTAAAGATCTTCCCTTTTCGGACCAAAGGTTAAATGGAAAGATCCCAAAGATGTTGCCCCATCGTCTTTTTCCTCATGCAAAGTATTCCATTTGGGTGGACTCAAAGTCCCAATTTAGGAGGGACCCTTTAGGTGTGCTCGAAGCCCTGCTTTGGCGTTCAAATTCTGTACTTGCGATCTCAGAACATGGAGCACGGAGTAGCGTTTATGATGAGGCGAAGGCTGTTGTAAAAAAAAACAAGGCCAAACCAGAAGAAGTTGAGGTGCAGTTGACTCAATACCGACATGATGGCTTGCCAGAAGACAAGAGGTTCAACGGAAAGAAGGCTTTATCTGAAGCATCTGTTATCGTGAGGGAGCATACACCATTGACTAATATGTTTATGTGCCTCTGGTTTAACGAGGTGGTTCGATTCACTTCTCGTGACCAGCTTAGTTTCCCATATGTATTATGGCGGCTGAAAGTGCTCAAGAACATCAACGTGTTCCCTGTATGTACCCGGAAAGATCTTGTTAATAGTATGGGTCACATACGCAAGGCTAAGCCACTGAGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616 Dirigent

Protein Analysis

477

Amino Acids

54.48

Weight (kDa)

9.45

Isoelectric Point (pI)

39.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 173 - 467 5.1e-119 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 861
AciI CCGC 3 cut(s) 47, 815, 1327
AclI AACGTT 1 cut(s) 370
AclWI GGATC 2 cut(s) 209, 907
AcoI YGGCCR 2 cut(s) 58, 100
AcsI RAATTY 3 cut(s) 319, 414, 1033
AcuI CTGAAG 2 cut(s) 414, 1227
AfaI GTAC 5 cut(s) 143, 668, 805, 1041, 1369
AfiI CCNNNNNNNGG 3 cut(s) 173, 987, 1023
AflIII ACRYGT 2 cut(s) 698, 1353
AgsI TTSAA 4 cut(s) 7, 325, 1032, 1186
AhdI GACNNNNNGTC 1 cut(s) 976
AjnI CCWGG 1 cut(s) 197
AjuI GAANNNNNNNTTGG 6 cut(s) 203, 235, 242, 274, 281, 313
AluBI AGCT 3 cut(s) 55, 133, 1303
AluI AGCT 3 cut(s) 55, 133, 1303
Alw21I GWGCWC 4 cut(s) 57, 1011, 1066, 1341
Alw26I GTCTC 1 cut(s) 37
AlwI GGATC 2 cut(s) 209, 907
AlwNI CAGNNNCTG 1 cut(s) 144
Ama87I CYCGRG 2 cut(s) 173, 345
AoxI GGCC 5 cut(s) 58, 100, 168, 525, 1113
ApoI RAATTY 3 cut(s) 319, 414, 1033
AspLEI GCGC 3 cut(s) 90, 151, 861
AspS9I GGNCC 3 cut(s) 169, 892, 994
AsuC2I CCSGG 1 cut(s) 1372
AsuHPI GGTGA 2 cut(s) 256, 295
AsuII TTCGAA 2 cut(s) 79, 760
AvaI CYCGRG 2 cut(s) 173, 345
AvaII GGWCC 2 cut(s) 892, 994
BanII GRGCYC 1 cut(s) 57
BauI CACGAG 1 cut(s) 1293
BbsI GAAGAC 2 cut(s) 663, 1179
Bbv12I GWGCWC 4 cut(s) 57, 1011, 1066, 1341
BccI CCATC 4 cut(s) 827, 833, 939, 1154
BcgI CGANNNNNNTGC 4 cut(s) 79, 113, 750, 784
BciT130I CCWGG 1 cut(s) 199
BclI TGATCA 1 cut(s) 334
BcnI CCSGG 1 cut(s) 1372
BcoDI GTCTC 1 cut(s) 37
BfaI CTAG 2 cut(s) 126, 563
BfmI CTRYAG 1 cut(s) 622
BfoI RGCGCY 1 cut(s) 152
BglII AGATCT 2 cut(s) 877, 1378
BisI GCNGC 2 cut(s) 816, 1328
BlpI GCTNAGC 1 cut(s) 1413
BlsI GCNGC 2 cut(s) 817, 1329
Bme1390I CCNGG 2 cut(s) 199, 1372
Bme18I GGWCC 2 cut(s) 892, 994
BmeRI GACNNNNNGTC 1 cut(s) 976
BmeT110I CYCGRG 2 cut(s) 173, 345
BmgT120I GGNCC 3 cut(s) 169, 892, 994
BmiI GGNNCC 2 cut(s) 995, 996
BmrFI CCNGG 2 cut(s) 199, 1372
BmrI ACTGGG 1 cut(s) 154
BmsI GCATC 3 cut(s) 304, 761, 1220
BmuI ACTGGG 1 cut(s) 154
BpiI GAAGAC 2 cut(s) 663, 1179
BplI GAGNNNNNCTC 2 cut(s) 293, 325
Bpu1102I GCTNAGC 1 cut(s) 1413
Bpu14I TTCGAA 2 cut(s) 79, 760
BpuEI CTTGAG 4 cut(s) 95, 422, 495, 1325
BpuMI CCSGG 1 cut(s) 1372
BsaBI GATNNNNATC 1 cut(s) 726
BsaJI CCNNGG 1 cut(s) 172
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 987, 1023
Bse1I ACTGG 2 cut(s) 149, 553
Bse8I GATNNNNATC 1 cut(s) 726
BseBI CCWGG 1 cut(s) 199
BseDI CCNNGG 1 cut(s) 172
BseGI GGATG 4 cut(s) 69, 319, 793, 844
BseJI GATNNNNATC 1 cut(s) 726
BseLI CCNNNNNNNGG 3 cut(s) 173, 987, 1023
BseMII CTCAG 3 cut(s) 42, 1065, 1412
BseNI ACTGG 2 cut(s) 149, 553
BseX3I CGGCCG 1 cut(s) 58
BsgI GTGCAG 1 cut(s) 1157
Bsh1236I CGCG 1 cut(s) 861
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 5 cut(s) 60, 102, 170, 527, 1115
BsiEI CGRYCG 1 cut(s) 61
BsiHKAI GWGCWC 4 cut(s) 57, 1011, 1066, 1341
BsiHKCI CYCGRG 2 cut(s) 173, 345
BsiSI CCGG 3 cut(s) 61, 167, 1372
BslFI GGGAC 2 cut(s) 964, 1007
BslI CCNNNNNNNGG 3 cut(s) 173, 987, 1023
BsmAI GTCTC 1 cut(s) 37
BsmFI GGGAC 2 cut(s) 964, 1007
BsnI GGCC 5 cut(s) 60, 102, 170, 527, 1115
BsoBI CYCGRG 2 cut(s) 173, 345
Bsp119I TTCGAA 2 cut(s) 79, 760
Bsp1286I GDGCHC 4 cut(s) 57, 1011, 1066, 1341
Bsp143I GATC 9 cut(s) 201, 334, 464, 565, 721, 877, 912, 1047, 1378
Bsp1720I GCTNAGC 1 cut(s) 1413
BspACI CCGC 3 cut(s) 47, 815, 1327
BspANI GGCC 5 cut(s) 60, 102, 170, 527, 1115
BspCNI CTCAG 3 cut(s) 41, 1064, 1413
BspFNI CGCG 1 cut(s) 861
BspLI GGNNCC 2 cut(s) 995, 996
BspPI GGATC 2 cut(s) 209, 907
BspT104I TTCGAA 2 cut(s) 79, 760
BsrI ACTGG 2 cut(s) 149, 553
BssECI CCNNGG 1 cut(s) 172
BssMI GATC 9 cut(s) 201, 334, 464, 565, 721, 877, 912, 1047, 1378
BssSI CACGAG 1 cut(s) 1293
Bst2BI CACGAG 1 cut(s) 1293
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 6 cut(s) 244, 283, 503, 601, 614, 626
Bst6I CTCTTC 1 cut(s) 423
BstBI TTCGAA 2 cut(s) 79, 760
BstC8I GCNNGC 2 cut(s) 104, 1166
BstDEI CTNAG 6 cut(s) 28, 800, 1051, 1304, 1413, 1421
BstF5I GGATG 4 cut(s) 69, 319, 793, 844
BstFNI CGCG 1 cut(s) 861
BstH2I RGCGCY 1 cut(s) 152
BstHHI GCGC 3 cut(s) 90, 151, 861
BstKTI GATC 9 cut(s) 204, 337, 467, 568, 724, 880, 915, 1050, 1381
BstMAI GTCTC 1 cut(s) 37
BstMBI GATC 9 cut(s) 201, 334, 464, 565, 721, 877, 912, 1047, 1378
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 2 cut(s) 112, 1336
BstNI CCWGG 1 cut(s) 199
BstNSI RCATGY 1 cut(s) 702
BstSCI CCNGG 2 cut(s) 197, 1370
BstSFI CTRYAG 1 cut(s) 622
BstUI CGCG 1 cut(s) 861
BstV2I GAAGAC 2 cut(s) 663, 1179
BstX2I RGATCY 3 cut(s) 877, 912, 1378
BstXI CCANNNNNNTGG 1 cut(s) 967
BstYI RGATCY 3 cut(s) 877, 912, 1378
BstZI CGGCCG 1 cut(s) 58
BsuRI GGCC 5 cut(s) 60, 102, 170, 527, 1115
BtsCI GGATG 4 cut(s) 69, 319, 793, 844
BtsIMutI CAGTG 2 cut(s) 631, 1418
Cac8I GCNNGC 2 cut(s) 104, 1166
CaiI CAGNNNCTG 1 cut(s) 144
CfoI GCGC 3 cut(s) 90, 151, 861
Cfr13I GGNCC 3 cut(s) 169, 892, 994
Csp6I GTAC 5 cut(s) 142, 667, 804, 1040, 1368
CviAII CATG 5 cut(s) 670, 699, 947, 1058, 1157
CviQI GTAC 5 cut(s) 142, 667, 804, 1040, 1368
DdeI CTNAG 6 cut(s) 28, 800, 1051, 1304, 1413, 1421
DpnI GATC 9 cut(s) 203, 336, 466, 567, 723, 879, 914, 1049, 1380
DpnII GATC 9 cut(s) 201, 334, 464, 565, 721, 877, 912, 1047, 1378
DraI TTTAAA 1 cut(s) 643
DriI GACNNNNNGTC 1 cut(s) 976
EaeI YGGCCR 2 cut(s) 58, 100
EagI CGGCCG 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 423
Eam1105I GACNNNNNGTC 1 cut(s) 976
EarI CTCTTC 1 cut(s) 423
Ecl136II GAGCTC 1 cut(s) 55
EclXI CGGCCG 1 cut(s) 58
Eco24I GRGCYC 1 cut(s) 57
Eco47I GGWCC 2 cut(s) 892, 994
Eco52I CGGCCG 1 cut(s) 58
Eco53kI GAGCTC 1 cut(s) 55
Eco57I CTGAAG 2 cut(s) 414, 1227
Eco88I CYCGRG 2 cut(s) 173, 345
EcoICRI GAGCTC 1 cut(s) 55
EcoO109I RGGNCCY 1 cut(s) 994
EcoRII CCWGG 1 cut(s) 197
EcoT38I GRGCYC 1 cut(s) 57
FaeI CATG 5 cut(s) 673, 702, 950, 1061, 1160
FalI AAGNNNNNCTT 2 cut(s) 1005, 1037
FaqI GGGAC 2 cut(s) 964, 1007
FatI CATG 5 cut(s) 669, 698, 946, 1057, 1156
FauNDI CATATG 1 cut(s) 1315
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 2 cut(s) 816, 1328
FokI GGATG 4 cut(s) 76, 326, 800, 851
FriOI GRGCYC 1 cut(s) 57
Fsp4HI GCNGC 2 cut(s) 816, 1328
FspBI CTAG 2 cut(s) 126, 563
GlaI GCGC 3 cut(s) 89, 150, 860
GluI GCNGC 2 cut(s) 816, 1328
HaeII RGCGCY 1 cut(s) 152
HaeIII GGCC 5 cut(s) 60, 102, 170, 527, 1115
HapII CCGG 3 cut(s) 61, 167, 1372
HhaI GCGC 3 cut(s) 90, 151, 861
Hin1II CATG 5 cut(s) 673, 702, 950, 1061, 1160
Hin6I GCGC 3 cut(s) 88, 149, 859
HinP1I GCGC 3 cut(s) 88, 149, 859
HincII GTYRAC 1 cut(s) 1143
HindII GTYRAC 1 cut(s) 1143
HpaII CCGG 3 cut(s) 61, 167, 1372
HphI GGTGA 2 cut(s) 256, 295
Hpy166II GTNNAC 4 cut(s) 696, 806, 970, 1143
Hpy188III TCNNGA 5 cut(s) 392, 512, 1222, 1295, 1342
Hpy8I GTNNAC 4 cut(s) 696, 806, 970, 1143
Hpy99I CGWCG 2 cut(s) 41, 44
HpyAV CCTTC 2 cut(s) 1084, 1189
HpyCH4III ACNGT 6 cut(s) 244, 283, 503, 601, 614, 626
HpyCH4IV ACGT 3 cut(s) 274, 370, 1353
HpyCH4V TGCA 4 cut(s) 681, 704, 950, 1138
HpyF10VI GCNNNNNNNGC 2 cut(s) 112, 1336
HpyF3I CTNAG 6 cut(s) 28, 800, 1051, 1304, 1413, 1421
HpySE526I ACGT 3 cut(s) 274, 370, 1353
Hsp92II CATG 5 cut(s) 673, 702, 950, 1061, 1160
HspAI GCGC 3 cut(s) 88, 149, 859
KflI GGGWCCC 1 cut(s) 994
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 9 cut(s) 201, 334, 464, 565, 721, 877, 912, 1047, 1378
LmnI GCTCC 2 cut(s) 1061, 1228
LweI GCATC 3 cut(s) 304, 761, 1220
MaeI CTAG 2 cut(s) 126, 563
MaeII ACGT 3 cut(s) 274, 370, 1353
MaeIII GTNAC 3 cut(s) 601, 1295, 1397
MalI GATC 9 cut(s) 203, 336, 466, 567, 723, 879, 914, 1049, 1380
MboI GATC 9 cut(s) 201, 334, 464, 565, 721, 877, 912, 1047, 1378
MflI RGATCY 3 cut(s) 877, 912, 1378
MhlI GDGCHC 4 cut(s) 57, 1011, 1066, 1341
MluCI AATT 6 cut(s) 319, 414, 538, 828, 983, 1033
MlyI GAGTC 8 cut(s) 78, 102, 236, 275, 337, 440, 965, 1138
MmeI TCCRAC 4 cut(s) 60, 210, 249, 334
MseI TTAA 8 cut(s) 447, 537, 585, 642, 873, 902, 1269, 1386
MslI CAYNNNNRTG 2 cut(s) 965, 1352
MspI CCGG 3 cut(s) 61, 167, 1372
MspR9I CCNGG 2 cut(s) 199, 1372
MvaI CCWGG 1 cut(s) 199
MvnI CGCG 1 cut(s) 861
MwoI GCNNNNNNNGC 2 cut(s) 112, 1336
NciI CCSGG 1 cut(s) 1372
NdeI CATATG 1 cut(s) 1315
NdeII GATC 9 cut(s) 201, 334, 464, 565, 721, 877, 912, 1047, 1378
NlaIII CATG 5 cut(s) 673, 702, 950, 1061, 1160
NlaIV GGNNCC 2 cut(s) 995, 996
NmeAIII GCCGAG 5 cut(s) 36, 78, 240, 279, 318
NmuCI GTSAC 2 cut(s) 1295, 1397
NspI RCATGY 1 cut(s) 702
NspV TTCGAA 2 cut(s) 79, 760
PciI ACATGT 1 cut(s) 698
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PfeI GAWTC 3 cut(s) 120, 325, 1284
PfoI TCCNGGA 1 cut(s) 197
PkrI GCNGC 2 cut(s) 817, 1329
PleI GAGTC 8 cut(s) 77, 102, 235, 274, 337, 439, 965, 1138
PpsI GAGTC 8 cut(s) 77, 102, 235, 274, 337, 439, 965, 1138
PpuMI RGGWCCY 1 cut(s) 994
PscI ACATGT 1 cut(s) 698
Psp124BI GAGCTC 1 cut(s) 57
Psp1406I AACGTT 1 cut(s) 370
Psp5II RGGWCCY 1 cut(s) 994
Psp6I CCWGG 1 cut(s) 197
PspGI CCWGG 1 cut(s) 197
PspN4I GGNNCC 2 cut(s) 995, 996
PspPI GGNCC 3 cut(s) 169, 892, 994
PspPPI RGGWCCY 1 cut(s) 994
PstNI CAGNNNCTG 1 cut(s) 144
PsuI RGATCY 3 cut(s) 877, 912, 1378
RsaI GTAC 5 cut(s) 143, 668, 805, 1041, 1369
RsaNI GTAC 5 cut(s) 142, 667, 804, 1040, 1368
RseI CAYNNNNRTG 2 cut(s) 965, 1352
SacI GAGCTC 1 cut(s) 57
SaqAI TTAA 8 cut(s) 447, 537, 585, 642, 873, 902, 1269, 1386
SatI GCNGC 2 cut(s) 816, 1328
Sau3AI GATC 9 cut(s) 201, 334, 464, 565, 721, 877, 912, 1047, 1378
Sau96I GGNCC 3 cut(s) 169, 892, 994
SchI GAGTC 8 cut(s) 78, 102, 236, 275, 337, 440, 965, 1138
ScrFI CCNGG 2 cut(s) 199, 1372
SduI GDGCHC 4 cut(s) 57, 1011, 1066, 1341
SfaNI GCATC 3 cut(s) 304, 761, 1220
SfcI CTRYAG 1 cut(s) 622
SfuI TTCGAA 2 cut(s) 79, 760
SinI GGWCC 2 cut(s) 892, 994
SmiMI CAYNNNNRTG 2 cut(s) 965, 1352
SmlI CTYRAG 4 cut(s) 110, 401, 510, 1340
SmoI CTYRAG 4 cut(s) 110, 401, 510, 1340
Sse9I AATT 6 cut(s) 319, 414, 538, 828, 983, 1033
SsiI CCGC 3 cut(s) 47, 815, 1327
SspMI CTAG 2 cut(s) 126, 563
SstI GAGCTC 1 cut(s) 57
StyD4I CCNGG 2 cut(s) 197, 1370
TaaI ACNGT 6 cut(s) 244, 283, 503, 601, 614, 626
TaiI ACGT 3 cut(s) 277, 373, 1356
TaqI TCGA 6 cut(s) 79, 189, 411, 760, 1011, 1282
TasI AATT 6 cut(s) 319, 414, 538, 828, 983, 1033
TatI WGTACW 3 cut(s) 666, 803, 1039
TauI GCSGC 2 cut(s) 818, 1330
TfiI GAWTC 3 cut(s) 120, 325, 1284
Tru1I TTAA 8 cut(s) 447, 537, 585, 642, 873, 902, 1269, 1386
Tru9I TTAA 8 cut(s) 447, 537, 585, 642, 873, 902, 1269, 1386
TscAI CASTG 2 cut(s) 631, 1425
TseFI GTSAC 2 cut(s) 1295, 1397
Tsp45I GTSAC 2 cut(s) 1295, 1397
TspDTI ATGAA 5 cut(s) 531, 609, 623, 669, 686
TspGWI ACGGA 2 cut(s) 1081, 1203
TspRI CASTG 2 cut(s) 631, 1425
VpaK11BI GGWCC 2 cut(s) 892, 994
XapI RAATTY 3 cut(s) 319, 414, 1033
XceI RCATGY 1 cut(s) 702
XcmI CCANNNNNNNNNTGG 2 cut(s) 902, 1320
XspI CTAG 2 cut(s) 126, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.