Rroxscaffold_4G00329420

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002998
Physical Location & Seq
Forward (+)
62741211 .. 62744331
3121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00329420.1

Sequence Viewer

Length: 1305 bp
ATGTTCAACAACAACAGCGTATCAATCTCAGTCTCCGATGACGAATCCGACGAGCTCGGCCGGATGCGCGTCCGAGTTCGCCGGAAGCGCAAGAAACTCGGCCACCGATTCAAGAACGAGTTCCTCCGCCGAGTGGTGAGGAGTCTGGTCAAGTACTGGGCCCTTCTGATTCTTCTGCCGGCCATTGGTTTGCTTCTGTATGAGGCTTGGAGTATCGGCTGGAAATCCCCACAGCTCAATAATAAGTCGGAAAAGCCGAGTTTGGTCCACTCGGAACTCAATGCGAGTTCGGGTAAGAAATCCGAAGGCAATTTGAATAGGCTTGATCCTTCCACTCGGGTTATTCACGGCGTTAGGCAACGTTGCTTGCAAATCTTACCTCCTGAAGAACTTGAGCATCTGGAAATTCCTGTGCGTGAAGATGCCACTAGTCCTGTTAAGAAATTGTTATACAGAGCGGAGAATGATGGTCTGTTGTTAGGAGGGTACAACACCCGGTCTGAAGAGCACACAGAGGCCACAAGATTTAATTTGTTTACGGGAAATCAAACTCTAGATCAGAGAGGTGAAATGTTTAAGGTAAATGAAACTGTTACCATAAACTGTGGTTTCTATAGTGAAAATGGTGGGTTTAAGATCTCAAATGAAGACAAGAATTACATGCAAAGTTGTGACATTGTAGTCTCCACTTGTGCATTCGGTGGTGGAGATGATCTTTATCAACCCATTGGAATGTCAGAGGCATCTCTTCAGAAGGTTTGCTATGTTGCTTTTTGGGATGAAGTTACTTTAAGTACTCAGGAATCAGCTGAACATAGAATTGGTGAGGATGGATTTATTGGGAGATGGCGCATTGTGGTTGTAAGCGATCTGCCTTTTGCGGACCAAAGGTTAAATGGAAAAATCCCAAAGATGTTGGCGCATCGCCTTTTTCCTCATTCAAAGTATTCCATTTGGGTAGATTCGAAGTCCCAATTTAGGAGGGACCCTTTAGGTGTTCTCGAAGCCCTTCTTTGGCGTACCAATTCAGTACTTGCGATCTCAGAACATGGAGCTCGCAGTAGTGTTTATGATGAGGCAAAGGCCGTTGTCAAGAAAAACAAGGCCAAACCGGAAGAAGTTGAGGTACAGTTGGCTCAGTACCGTCGTGATGGATTGCCAGAAGACAAGAGGTTCAATGGAAAGAAAGGTGGTTCGTTTTACGTCTCGCGATCAGCTCAGTTTCCCATATGTACTGTGGAGGTTGAAAGTGCTCAAGAACATCAACATGTTCTCTGTAGTGCTTCAATTTCCCAATCTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616 Dirigent

Protein Analysis

434

Amino Acids

49.21

Weight (kDa)

8.53

Isoelectric Point (pI)

48.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 167 - 396 1.3e-86 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 680
AccBSI CCGCTC 1 cut(s) 458
AccII CGCG 2 cut(s) 69, 1210
AciI CCGC 3 cut(s) 127, 458, 881
AclI AACGTT 1 cut(s) 361
AclWI GGATC 1 cut(s) 320
AcoI YGGCCR 3 cut(s) 58, 100, 180
AcsI RAATTY 1 cut(s) 405
AcuI CTGAAG 3 cut(s) 405, 522, 734
AfaI GTAC 8 cut(s) 155, 488, 796, 1021, 1032, 1128, 1142, 1234
AfiI CCNNNNNNNGG 4 cut(s) 133, 185, 978, 1014
AflIII ACRYGT 1 cut(s) 1267
AgsI TTSAA 7 cut(s) 7, 112, 316, 942, 1177, 1247, 1287
AhlI ACTAGT 1 cut(s) 428
AjuI GAANNNNNNNTTGG 2 cut(s) 804, 836
AluBI AGCT 5 cut(s) 55, 235, 809, 1055, 1217
AluI AGCT 5 cut(s) 55, 235, 809, 1055, 1217
Alw21I GWGCWC 4 cut(s) 57, 510, 1057, 1255
Alw26I GTCTC 3 cut(s) 37, 688, 1210
AlwI GGATC 1 cut(s) 320
Ama87I CYCGRG 1 cut(s) 336
AoxI GGCC 7 cut(s) 58, 100, 159, 180, 516, 1083, 1104
ApaI GGGCCC 1 cut(s) 163
ApoI RAATTY 1 cut(s) 405
Asp700I GAANNNNTTC 2 cut(s) 119, 1008
AspLEI GCGC 4 cut(s) 69, 90, 852, 922
AspS9I GGNCC 5 cut(s) 159, 160, 265, 883, 985
AsuC2I CCSGG 1 cut(s) 496
AsuHPI GGTGA 3 cut(s) 148, 578, 836
AsuII TTCGAA 1 cut(s) 965
AvaI CYCGRG 1 cut(s) 336
AvaII GGWCC 3 cut(s) 265, 883, 985
BaeGI GKGCMC 1 cut(s) 163
BanII GRGCYC 3 cut(s) 57, 163, 1057
BarI GAAGNNNNNNTAC 2 cut(s) 1110, 1142
BbsI GAAGAC 2 cut(s) 654, 1170
Bbv12I GWGCWC 4 cut(s) 57, 510, 1057, 1255
BccI CCATC 4 cut(s) 461, 824, 840, 1145
BceAI ACGGC 2 cut(s) 364, 1070
BcgI CGANNNNNNTGC 2 cut(s) 79, 113
BcnI CCSGG 1 cut(s) 496
BcoDI GTCTC 3 cut(s) 37, 688, 1210
BcuI ACTAGT 1 cut(s) 428
BfaI CTAG 2 cut(s) 429, 554
BfmI CTRYAG 2 cut(s) 613, 1276
BglII AGATCT 1 cut(s) 636
BmcAI AGTACT 3 cut(s) 155, 796, 1032
Bme1390I CCNGG 1 cut(s) 496
Bme18I GGWCC 3 cut(s) 265, 883, 985
BmeT110I CYCGRG 1 cut(s) 336
BmgT120I GGNCC 5 cut(s) 159, 160, 265, 883, 985
BmiI GGNNCC 3 cut(s) 161, 986, 987
BmrFI CCNGG 1 cut(s) 496
BmrI ACTGGG 1 cut(s) 166
BmsI GCATC 5 cut(s) 54, 406, 412, 752, 931
BmuI ACTGGG 1 cut(s) 166
BpiI GAAGAC 2 cut(s) 654, 1170
Bpu14I TTCGAA 1 cut(s) 965
BpuEI CTTGAG 2 cut(s) 413, 1239
BpuMI CCSGG 1 cut(s) 496
BsaBI GATNNNNATC 1 cut(s) 717
BsaWI WCCGGW 1 cut(s) 1111
Bsc4I CCNNNNNNNGG 4 cut(s) 133, 185, 978, 1014
Bse118I RCCGGY 1 cut(s) 178
Bse1I ACTGG 1 cut(s) 161
Bse8I GATNNNNATC 1 cut(s) 717
BseGI GGATG 3 cut(s) 69, 784, 835
BseJI GATNNNNATC 1 cut(s) 717
BseLI CCNNNNNNNGG 4 cut(s) 133, 185, 978, 1014
BseMII CTCAG 5 cut(s) 42, 812, 1056, 1151, 1232
BseNI ACTGG 1 cut(s) 161
BseRI GAGGAG 1 cut(s) 154
BseSI GKGCMC 1 cut(s) 163
BseX3I CGGCCG 1 cut(s) 58
Bsh1236I CGCG 2 cut(s) 69, 1210
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 7 cut(s) 60, 102, 161, 182, 518, 1085, 1106
BsiEI CGRYCG 1 cut(s) 61
BsiHKAI GWGCWC 4 cut(s) 57, 510, 1057, 1255
BsiHKCI CYCGRG 1 cut(s) 336
BsiSI CCGG 5 cut(s) 61, 82, 179, 496, 1112
BslFI GGGAC 2 cut(s) 955, 998
BslI CCNNNNNNNGG 4 cut(s) 133, 185, 978, 1014
BsmAI GTCTC 3 cut(s) 37, 688, 1210
BsmBI CGTCTC 1 cut(s) 1210
BsmFI GGGAC 2 cut(s) 955, 998
BsmI GAATGC 1 cut(s) 695
BsnI GGCC 7 cut(s) 60, 102, 161, 182, 518, 1085, 1106
BsoBI CYCGRG 1 cut(s) 336
Bsp119I TTCGAA 1 cut(s) 965
Bsp120I GGGCCC 1 cut(s) 159
Bsp1286I GDGCHC 5 cut(s) 57, 163, 510, 1057, 1255
Bsp143I GATC 7 cut(s) 325, 556, 636, 712, 868, 1038, 1211
Bsp68I TCGCGA 1 cut(s) 1210
BspACI CCGC 3 cut(s) 127, 458, 881
BspANI GGCC 7 cut(s) 60, 102, 161, 182, 518, 1085, 1106
BspCNI CTCAG 5 cut(s) 41, 811, 1055, 1150, 1231
BspFNI CGCG 2 cut(s) 69, 1210
BspLI GGNNCC 3 cut(s) 161, 986, 987
BspPI GGATC 1 cut(s) 320
BspQI GCTCTTC 1 cut(s) 498
BspT104I TTCGAA 1 cut(s) 965
BsrBI CCGCTC 1 cut(s) 458
BsrFI RCCGGY 1 cut(s) 178
BsrI ACTGG 1 cut(s) 161
BssAI RCCGGY 1 cut(s) 178
BssMI GATC 7 cut(s) 325, 556, 636, 712, 868, 1038, 1211
Bst4CI ACNGT 5 cut(s) 592, 605, 1131, 1145, 1237
Bst6I CTCTTC 2 cut(s) 498, 753
BstBI TTCGAA 1 cut(s) 965
BstC8I GCNNGC 3 cut(s) 180, 368, 1057
BstDEI CTNAG 5 cut(s) 28, 798, 1042, 1137, 1218
BstF5I GGATG 3 cut(s) 69, 784, 835
BstFNI CGCG 2 cut(s) 69, 1210
BstHHI GCGC 4 cut(s) 69, 90, 852, 922
BstKTI GATC 7 cut(s) 328, 559, 639, 715, 871, 1041, 1214
BstMAI GTCTC 3 cut(s) 37, 688, 1210
BstMBI GATC 7 cut(s) 325, 556, 636, 712, 868, 1038, 1211
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 2 cut(s) 66, 87
BstNSI RCATGY 2 cut(s) 664, 1271
BstSCI CCNGG 1 cut(s) 494
BstSFI CTRYAG 2 cut(s) 613, 1276
BstSLI GKGCMC 1 cut(s) 163
BstUI CGCG 2 cut(s) 69, 1210
BstV2I GAAGAC 2 cut(s) 654, 1170
BstX2I RGATCY 1 cut(s) 636
BstYI RGATCY 1 cut(s) 636
BstZI CGGCCG 1 cut(s) 58
BsuRI GGCC 7 cut(s) 60, 102, 161, 182, 518, 1085, 1106
BtgZI GCGATG 1 cut(s) 908
BtsCI GGATG 3 cut(s) 69, 784, 835
BtuMI TCGCGA 1 cut(s) 1210
Cac8I GCNNGC 3 cut(s) 180, 368, 1057
CfoI GCGC 4 cut(s) 69, 90, 852, 922
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 5 cut(s) 159, 160, 265, 883, 985
CseI GACGC 1 cut(s) 58
Csp6I GTAC 8 cut(s) 154, 487, 795, 1020, 1031, 1127, 1141, 1233
CviAII CATG 3 cut(s) 661, 1049, 1268
CviQI GTAC 8 cut(s) 154, 487, 795, 1020, 1031, 1127, 1141, 1233
DdeI CTNAG 5 cut(s) 28, 798, 1042, 1137, 1218
DpnI GATC 7 cut(s) 327, 558, 638, 714, 870, 1040, 1213
DpnII GATC 7 cut(s) 325, 556, 636, 712, 868, 1038, 1211
DrdI GACNNNNNNGTC 1 cut(s) 680
DseDI GACNNNNNNGTC 1 cut(s) 680
EaeI YGGCCR 3 cut(s) 58, 100, 180
EagI CGGCCG 1 cut(s) 58
Eam1104I CTCTTC 2 cut(s) 498, 753
EarI CTCTTC 2 cut(s) 498, 753
EciI GGCGGA 1 cut(s) 116
Ecl136II GAGCTC 2 cut(s) 55, 1055
EclXI CGGCCG 1 cut(s) 58
Eco24I GRGCYC 3 cut(s) 57, 163, 1057
Eco47I GGWCC 3 cut(s) 265, 883, 985
Eco52I CGGCCG 1 cut(s) 58
Eco53kI GAGCTC 2 cut(s) 55, 1055
Eco57I CTGAAG 3 cut(s) 405, 522, 734
Eco88I CYCGRG 1 cut(s) 336
EcoICRI GAGCTC 2 cut(s) 55, 1055
EcoO109I RGGNCCY 2 cut(s) 160, 985
EcoT38I GRGCYC 3 cut(s) 57, 163, 1057
Esp3I CGTCTC 1 cut(s) 1210
FaeI CATG 3 cut(s) 664, 1052, 1271
FalI AAGNNNNNCTT 2 cut(s) 996, 1028
FaqI GGGAC 2 cut(s) 955, 998
FatI CATG 3 cut(s) 660, 1048, 1267
FauNDI CATATG 1 cut(s) 1229
FokI GGATG 3 cut(s) 76, 791, 842
FriOI GRGCYC 3 cut(s) 57, 163, 1057
FspBI CTAG 2 cut(s) 429, 554
GlaI GCGC 4 cut(s) 68, 89, 851, 921
HaeIII GGCC 7 cut(s) 60, 102, 161, 182, 518, 1085, 1106
HapII CCGG 5 cut(s) 61, 82, 179, 496, 1112
HgaI GACGC 1 cut(s) 58
HhaI GCGC 4 cut(s) 69, 90, 852, 922
Hin1II CATG 3 cut(s) 664, 1052, 1271
Hin6I GCGC 4 cut(s) 67, 88, 850, 920
HinP1I GCGC 4 cut(s) 67, 88, 850, 920
HinfI GANTC 6 cut(s) 44, 108, 142, 169, 803, 962
HpaII CCGG 5 cut(s) 61, 82, 179, 496, 1112
HphI GGTGA 3 cut(s) 148, 578, 836
Hpy166II GTNNAC 2 cut(s) 268, 537
Hpy8I GTNNAC 2 cut(s) 268, 537
Hpy99I CGWCG 2 cut(s) 53, 1149
HpyAV CCTTC 5 cut(s) 173, 299, 339, 748, 1019
HpyCH4III ACNGT 5 cut(s) 592, 605, 1131, 1145, 1237
HpyCH4IV ACGT 2 cut(s) 361, 1203
HpyCH4V TGCA 3 cut(s) 370, 664, 695
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 87
HpyF3I CTNAG 5 cut(s) 28, 798, 1042, 1137, 1218
HpySE526I ACGT 2 cut(s) 361, 1203
Hsp92II CATG 3 cut(s) 664, 1052, 1271
HspAI GCGC 4 cut(s) 67, 88, 850, 920
KflI GGGWCCC 1 cut(s) 985
KroI GCCGGC 1 cut(s) 178
KroNI GCCGGC 1 cut(s) 180
Kzo9I GATC 7 cut(s) 325, 556, 636, 712, 868, 1038, 1211
LguI GCTCTTC 1 cut(s) 498
LmnI GCTCC 1 cut(s) 1052
LweI GCATC 5 cut(s) 54, 406, 412, 752, 931
MaeI CTAG 2 cut(s) 429, 554
MaeII ACGT 2 cut(s) 361, 1203
MaeIII GTNAC 3 cut(s) 592, 671, 784
MalI GATC 7 cut(s) 327, 558, 638, 714, 870, 1040, 1213
MbiI CCGCTC 1 cut(s) 458
MboI GATC 7 cut(s) 325, 556, 636, 712, 868, 1038, 1211
MboII GAAGA 8 cut(s) 164, 398, 431, 515, 659, 740, 1127, 1175
MflI RGATCY 1 cut(s) 636
MhlI GDGCHC 5 cut(s) 57, 163, 510, 1057, 1255
MluCI AATT 9 cut(s) 310, 405, 443, 529, 655, 819, 974, 1024, 1287
MlyI GAGTC 1 cut(s) 151
MmeI TCCRAC 2 cut(s) 72, 228
MroNI GCCGGC 1 cut(s) 178
MroXI GAANNNNTTC 2 cut(s) 119, 1008
MseI TTAA 6 cut(s) 438, 528, 576, 633, 791, 893
MslI CAYNNNNRTG 2 cut(s) 731, 1266
MspA1I CMGCKG 1 cut(s) 809
MspI CCGG 5 cut(s) 61, 82, 179, 496, 1112
MspR9I CCNGG 1 cut(s) 496
Mva1269I GAATGC 1 cut(s) 695
MvnI CGCG 2 cut(s) 69, 1210
MwoI GCNNNNNNNGC 2 cut(s) 66, 87
NaeI GCCGGC 1 cut(s) 180
NciI CCSGG 1 cut(s) 496
NdeI CATATG 1 cut(s) 1229
NdeII GATC 7 cut(s) 325, 556, 636, 712, 868, 1038, 1211
NgoMIV GCCGGC 1 cut(s) 178
NlaIII CATG 3 cut(s) 664, 1052, 1271
NlaIV GGNNCC 3 cut(s) 161, 986, 987
NmeAIII GCCGAG 4 cut(s) 36, 78, 155, 282
NmuCI GTSAC 1 cut(s) 671
NruI TCGCGA 1 cut(s) 1210
NspI RCATGY 2 cut(s) 664, 1271
NspV TTCGAA 1 cut(s) 965
PciI ACATGT 1 cut(s) 1267
PciSI GCTCTTC 1 cut(s) 498
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 254
PctI GAATGC 1 cut(s) 695
PdiI GCCGGC 1 cut(s) 180
PdmI GAANNNNTTC 2 cut(s) 119, 1008
PfeI GAWTC 5 cut(s) 44, 108, 169, 803, 962
PleI GAGTC 1 cut(s) 150
PpsI GAGTC 1 cut(s) 150
PpuMI RGGWCCY 1 cut(s) 985
PscI ACATGT 1 cut(s) 1267
Psp124BI GAGCTC 2 cut(s) 57, 1057
Psp1406I AACGTT 1 cut(s) 361
Psp5II RGGWCCY 1 cut(s) 985
PspN4I GGNNCC 3 cut(s) 161, 986, 987
PspOMI GGGCCC 1 cut(s) 159
PspPI GGNCC 5 cut(s) 159, 160, 265, 883, 985
PspPPI RGGWCCY 1 cut(s) 985
PsuI RGATCY 1 cut(s) 636
PvuII CAGCTG 1 cut(s) 809
RruI TCGCGA 1 cut(s) 1210
RsaI GTAC 8 cut(s) 155, 488, 796, 1021, 1032, 1128, 1142, 1234
RsaNI GTAC 8 cut(s) 154, 487, 795, 1020, 1031, 1127, 1141, 1233
RseI CAYNNNNRTG 2 cut(s) 731, 1266
SacI GAGCTC 2 cut(s) 57, 1057
SapI GCTCTTC 1 cut(s) 498
SaqAI TTAA 6 cut(s) 438, 528, 576, 633, 791, 893
Sau3AI GATC 7 cut(s) 325, 556, 636, 712, 868, 1038, 1211
Sau96I GGNCC 5 cut(s) 159, 160, 265, 883, 985
ScaI AGTACT 3 cut(s) 155, 796, 1032
SchI GAGTC 1 cut(s) 151
ScrFI CCNGG 1 cut(s) 496
SduI GDGCHC 5 cut(s) 57, 163, 510, 1057, 1255
SfaNI GCATC 5 cut(s) 54, 406, 412, 752, 931
SfcI CTRYAG 2 cut(s) 613, 1276
SfuI TTCGAA 1 cut(s) 965
SinI GGWCC 3 cut(s) 265, 883, 985
SmiMI CAYNNNNRTG 2 cut(s) 731, 1266
SmlI CTYRAG 2 cut(s) 392, 1254
SmoI CTYRAG 2 cut(s) 392, 1254
SpeI ACTAGT 1 cut(s) 428
Sse9I AATT 9 cut(s) 310, 405, 443, 529, 655, 819, 974, 1024, 1287
SsiI CCGC 3 cut(s) 127, 458, 881
SspMI CTAG 2 cut(s) 429, 554
SstI GAGCTC 2 cut(s) 57, 1057
StyD4I CCNGG 1 cut(s) 494
TaaI ACNGT 5 cut(s) 592, 605, 1131, 1145, 1237
TaiI ACGT 2 cut(s) 364, 1206
TaqI TCGA 2 cut(s) 965, 1002
TasI AATT 9 cut(s) 310, 405, 443, 529, 655, 819, 974, 1024, 1287
TatI WGTACW 4 cut(s) 153, 794, 1030, 1232
TfiI GAWTC 5 cut(s) 44, 108, 169, 803, 962
Tru1I TTAA 6 cut(s) 438, 528, 576, 633, 791, 893
Tru9I TTAA 6 cut(s) 438, 528, 576, 633, 791, 893
TseFI GTSAC 1 cut(s) 671
Tsp45I GTSAC 1 cut(s) 671
TspDTI ATGAA 3 cut(s) 600, 660, 795
VpaK11BI GGWCC 3 cut(s) 265, 883, 985
XapI RAATTY 1 cut(s) 405
XbaI TCTAGA 1 cut(s) 553
XceI RCATGY 2 cut(s) 664, 1271
XcmI CCANNNNNNNNNTGG 2 cut(s) 893, 1234
XmnI GAANNNNTTC 2 cut(s) 119, 1008
XspI CTAG 2 cut(s) 429, 554
ZrmI AGTACT 3 cut(s) 155, 796, 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.