Rh4AG214900

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002998
Physical Location & Seq
Reverse (-)
51055224 .. 51058173
2950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG214900.1

Sequence Viewer

Length: 1383 bp
ATGTTGAACAACAAGAGCATATCAATCGCAGTGTCGGATGACGAATCTGACGAACTCGGCCGGATGCGCGTTCAAGTTCGCCGGAAGCGCAAGAAACTCCACCACCGATTCAAGAACGGAGTGGTGAGGAATCTGGTCAGGTACTGGCCCCTGTTCTGGGCCCTTGTGATTTTTCTAGTAGCCATTGGTTTGCTTCTGTATGAGGCATGGACTATCGGCTGGAAATCCCCACAACTCAATAACTCAGAACTCGCTGCGGTGAACAATGATTTGAATCGGCTTGATCCTATCACTGAGGTTGTTCGTGGCGTTACGCAACGTTGCTTGCCAATCTTACCTCCTGAACAACTTGAGCATCTGGAAATTCCTGTGCCCCAAGATTCCACTAGTCCTGTTAAAAAATTGATATACACAACTGAGAATGATGCTCCGTTGTTAGGACGATGGTACAACACCCTGTCTCAAGAGCACACAGATGCAACAAGATTTAATTTGTTTACTGGAAATCAAACTCTAGATCAGAGAGGTGAAATGTTTAAGGTAAATGAAACTGTTACTATAAACTGTGGTTTCTACAGTGAAAGTGGTGGGTTTAAGATCTCAAATGAAGACAAGAATTACATGAAAAGTTGTGACATTATAGTCTCCACTTGTGCATTTGGTGGTGGAGATGATCTTTATCAACCCATTGGAATGTCAGAGGCATCCCTTCAGAAGGTTTGCTTTGTTGCTTTTTGGGATGAAGTTACTGTGAGTACTCAAGAGTCAGCTGAACATAGAATTGGTGAGGATGGATTTATTGGGAAATGGCGCATTGTGGTTGTAAGAGATCTCCCTTTTGCGGACCAAAGGTTAAATGGAAAAATCCCAAAGATGTTGGCGCATCGTCTTTTTCCTCATTCAAAGTATTCCATTTGGGTAGATTCGAAGTCCCAATTTAGAAGGGACCCTTTAGGTGTGCTCGAAGCTCTTCTCTGGCGTACAAATTCTGTACTTGCAATCTCAGAACATGGAGCTCGTAGTAGCATTTATGACGAGGCGAAGGCTGTTGTCAAGAAAAACAAGGCCAAACCTGAAGAAGTTAAGACGCAGTTGACTCAATATCGGAGTGATGGCTTGCCAGAAGACAAGCGATTCAATGGAAAGAAAGCTCTAGCGGAAGCATCTGTTATCGTGAGGGAGCATACCCCACTGACTAATATGTTTATGTGCCTCTGGTTCAACGAGGTAGTTCGTTTTACTTCTCGCGATCAGCTTAGTTTCCCGTATGTACTATGGCGGTTGAAAGTGCTCAAGAACATTAACATGTTCCCTGTATGCACCCGCAAAGATCTTGTTAATAGCATAGGCCACATTCGCAAGGCTATGCCTCTCAGTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616 Dirigent

Protein Analysis

460

Amino Acids

52.71

Weight (kDa)

9.13

Isoelectric Point (pI)

38.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 156 - 451 3.1e-119 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 641
AccII CGCG 2 cut(s) 69, 1248
AciI CCGC 5 cut(s) 257, 842, 1157, 1279, 1324
AclI AACGTT 1 cut(s) 319
AclWI GGATC 1 cut(s) 278
AcoI YGGCCR 1 cut(s) 58
AcsI RAATTY 2 cut(s) 363, 985
AcuI CTGAAG 2 cut(s) 695, 1095
AfaI GTAC 6 cut(s) 143, 449, 757, 982, 993, 1272
AfiI CCNNNNNNNGG 5 cut(s) 156, 157, 437, 715, 841
AflIII ACRYGT 1 cut(s) 1305
AgsI TTSAA 8 cut(s) 7, 74, 112, 274, 903, 1138, 1222, 1285
AhlI ACTAGT 1 cut(s) 386
AjuI GAANNNNNNNTTGG 2 cut(s) 765, 797
AluBI AGCT 5 cut(s) 770, 968, 1016, 1151, 1255
AluI AGCT 5 cut(s) 770, 968, 1016, 1151, 1255
Alw21I GWGCWC 4 cut(s) 471, 963, 1018, 1293
Alw26I GTCTC 2 cut(s) 465, 649
AlwI GGATC 1 cut(s) 278
AlwNI CAGNNNCTG 1 cut(s) 144
AoxI GGCC 5 cut(s) 58, 146, 159, 1065, 1348
ApaI GGGCCC 1 cut(s) 163
ApeKI GCWGC 1 cut(s) 254
ApoI RAATTY 2 cut(s) 363, 985
Asp700I GAANNNNTTC 1 cut(s) 969
AspLEI GCGC 4 cut(s) 69, 90, 813, 883
AspS9I GGNCC 5 cut(s) 147, 159, 160, 844, 946
AsuHPI GGTGA 4 cut(s) 136, 271, 539, 797
AsuII TTCGAA 1 cut(s) 926
AvaII GGWCC 2 cut(s) 844, 946
BaeGI GKGCMC 2 cut(s) 163, 375
BanII GRGCYC 2 cut(s) 163, 1018
BbsI GAAGAC 2 cut(s) 615, 1131
Bbv12I GWGCWC 4 cut(s) 471, 963, 1018, 1293
BbvI GCAGC 1 cut(s) 241
BccI CCATC 3 cut(s) 438, 785, 1106
BcgI CGANNNNNNTGC 2 cut(s) 7, 41
BcoDI GTCTC 2 cut(s) 465, 649
BcuI ACTAGT 1 cut(s) 386
BfaI CTAG 4 cut(s) 176, 387, 515, 1154
BfmI CTRYAG 1 cut(s) 574
BglII AGATCT 3 cut(s) 597, 829, 1330
BisI GCNGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 256
BmcAI AGTACT 1 cut(s) 757
Bme18I GGWCC 2 cut(s) 844, 946
BmgT120I GGNCC 5 cut(s) 147, 159, 160, 844, 946
BmiI GGNNCC 4 cut(s) 149, 161, 947, 948
BmsI GCATC 7 cut(s) 54, 364, 415, 466, 713, 892, 1172
BpiI GAAGAC 2 cut(s) 615, 1131
Bpu14I TTCGAA 1 cut(s) 926
BpuEI CTTGAG 4 cut(s) 371, 447, 744, 1277
BsaBI GATNNNNATC 2 cut(s) 273, 678
Bsc4I CCNNNNNNNGG 5 cut(s) 156, 157, 437, 715, 841
Bse1I ACTGG 2 cut(s) 149, 505
Bse8I GATNNNNATC 2 cut(s) 273, 678
BseGI GGATG 5 cut(s) 43, 69, 704, 745, 796
BseJI GATNNNNATC 2 cut(s) 273, 678
BseLI CCNNNNNNNGG 5 cut(s) 156, 157, 437, 715, 841
BseMII CTCAG 4 cut(s) 258, 285, 408, 1017
BseNI ACTGG 2 cut(s) 149, 505
BseSI GKGCMC 2 cut(s) 163, 375
BseX3I CGGCCG 1 cut(s) 58
BseXI GCAGC 1 cut(s) 241
Bsh1236I CGCG 2 cut(s) 69, 1248
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 5 cut(s) 60, 148, 161, 1067, 1350
BsiEI CGRYCG 1 cut(s) 61
BsiHKAI GWGCWC 4 cut(s) 471, 963, 1018, 1293
BsiSI CCGG 2 cut(s) 61, 82
BslFI GGGAC 2 cut(s) 916, 959
BslI CCNNNNNNNGG 5 cut(s) 156, 157, 437, 715, 841
BsmAI GTCTC 2 cut(s) 465, 649
BsmFI GGGAC 2 cut(s) 916, 959
BsnI GGCC 5 cut(s) 60, 148, 161, 1067, 1350
Bsp119I TTCGAA 1 cut(s) 926
Bsp120I GGGCCC 1 cut(s) 159
Bsp1286I GDGCHC 6 cut(s) 163, 375, 471, 963, 1018, 1293
Bsp143I GATC 7 cut(s) 283, 517, 597, 673, 829, 1249, 1330
Bsp68I TCGCGA 1 cut(s) 1248
BspACI CCGC 5 cut(s) 257, 842, 1157, 1279, 1324
BspANI GGCC 5 cut(s) 60, 148, 161, 1067, 1350
BspCNI CTCAG 4 cut(s) 257, 286, 409, 1016
BspFNI CGCG 2 cut(s) 69, 1248
BspLI GGNNCC 4 cut(s) 149, 161, 947, 948
BspPI GGATC 1 cut(s) 278
BspQI GCTCTTC 1 cut(s) 975
BspT104I TTCGAA 1 cut(s) 926
BsrI ACTGG 2 cut(s) 149, 505
BssMI GATC 7 cut(s) 283, 517, 597, 673, 829, 1249, 1330
Bst4CI ACNGT 4 cut(s) 553, 566, 578, 751
Bst6I CTCTTC 1 cut(s) 975
BstBI TTCGAA 1 cut(s) 926
BstC8I GCNNGC 2 cut(s) 326, 1118
BstDEI CTNAG 6 cut(s) 244, 294, 417, 1003, 1256, 1373
BstENI CCTNNNNNAGG 1 cut(s) 713
BstF5I GGATG 5 cut(s) 43, 69, 704, 745, 796
BstFNI CGCG 2 cut(s) 69, 1248
BstHHI GCGC 4 cut(s) 69, 90, 813, 883
BstKTI GATC 7 cut(s) 286, 520, 600, 676, 832, 1252, 1333
BstMAI GTCTC 2 cut(s) 465, 649
BstMBI GATC 7 cut(s) 283, 517, 597, 673, 829, 1249, 1330
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 3 cut(s) 66, 87, 1356
BstNSI RCATGY 1 cut(s) 1309
BstSFI CTRYAG 1 cut(s) 574
BstSLI GKGCMC 2 cut(s) 163, 375
BstUI CGCG 2 cut(s) 69, 1248
BstV1I GCAGC 1 cut(s) 241
BstV2I GAAGAC 2 cut(s) 615, 1131
BstX2I RGATCY 3 cut(s) 597, 829, 1330
BstYI RGATCY 3 cut(s) 597, 829, 1330
BstZI CGGCCG 1 cut(s) 58
BsuRI GGCC 5 cut(s) 60, 148, 161, 1067, 1350
BtsCI GGATG 5 cut(s) 43, 69, 704, 745, 796
BtsI GCAGTG 1 cut(s) 36
BtsIMutI CAGTG 4 cut(s) 36, 291, 583, 1190
BtuMI TCGCGA 1 cut(s) 1248
Cac8I GCNNGC 2 cut(s) 326, 1118
CaiI CAGNNNCTG 1 cut(s) 144
CfoI GCGC 4 cut(s) 69, 90, 813, 883
Cfr13I GGNCC 5 cut(s) 147, 159, 160, 844, 946
CseI GACGC 1 cut(s) 1096
Csp6I GTAC 6 cut(s) 142, 448, 756, 981, 992, 1271
CviAII CATG 4 cut(s) 207, 622, 1010, 1306
CviQI GTAC 6 cut(s) 142, 448, 756, 981, 992, 1271
DdeI CTNAG 6 cut(s) 244, 294, 417, 1003, 1256, 1373
DpnI GATC 7 cut(s) 285, 519, 599, 675, 831, 1251, 1332
DpnII GATC 7 cut(s) 283, 517, 597, 673, 829, 1249, 1330
DrdI GACNNNNNNGTC 1 cut(s) 641
DseDI GACNNNNNNGTC 1 cut(s) 641
EaeI YGGCCR 1 cut(s) 58
EagI CGGCCG 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 975
EarI CTCTTC 1 cut(s) 975
Ecl136II GAGCTC 1 cut(s) 1016
EclXI CGGCCG 1 cut(s) 58
Eco24I GRGCYC 2 cut(s) 163, 1018
Eco47I GGWCC 2 cut(s) 844, 946
Eco52I CGGCCG 1 cut(s) 58
Eco53kI GAGCTC 1 cut(s) 1016
Eco57I CTGAAG 2 cut(s) 695, 1095
EcoICRI GAGCTC 1 cut(s) 1016
EcoNI CCTNNNNNAGG 1 cut(s) 713
EcoO109I RGGNCCY 2 cut(s) 160, 946
EcoT38I GRGCYC 2 cut(s) 163, 1018
FaeI CATG 4 cut(s) 210, 625, 1013, 1309
FalI AAGNNNNNCTT 4 cut(s) 707, 739, 934, 966
FaqI GGGAC 2 cut(s) 916, 959
FatI CATG 4 cut(s) 206, 621, 1009, 1305
FauI CCCGC 1 cut(s) 1331
Fnu4HI GCNGC 1 cut(s) 255
FokI GGATG 5 cut(s) 50, 76, 691, 752, 803
FriOI GRGCYC 2 cut(s) 163, 1018
Fsp4HI GCNGC 1 cut(s) 255
FspBI CTAG 4 cut(s) 176, 387, 515, 1154
GlaI GCGC 4 cut(s) 68, 89, 812, 882
GluI GCNGC 1 cut(s) 255
HaeIII GGCC 5 cut(s) 60, 148, 161, 1067, 1350
HapII CCGG 2 cut(s) 61, 82
HgaI GACGC 1 cut(s) 1096
HhaI GCGC 4 cut(s) 69, 90, 813, 883
Hin1II CATG 4 cut(s) 210, 625, 1013, 1309
Hin6I GCGC 4 cut(s) 67, 88, 811, 881
HinP1I GCGC 4 cut(s) 67, 88, 811, 881
HincII GTYRAC 1 cut(s) 1095
HindII GTYRAC 1 cut(s) 1095
HinfI GANTC 9 cut(s) 44, 108, 130, 274, 380, 764, 923, 1096, 1134
HpaII CCGG 2 cut(s) 61, 82
HphI GGTGA 4 cut(s) 136, 271, 539, 797
Hpy166II GTNNAC 3 cut(s) 262, 498, 1095
Hpy188I TCNGA 8 cut(s) 37, 49, 247, 522, 700, 714, 1006, 1107
Hpy8I GTNNAC 3 cut(s) 262, 498, 1095
HpyAV CCTTC 4 cut(s) 709, 719, 936, 1036
HpyCH4III ACNGT 4 cut(s) 553, 566, 578, 751
HpyCH4IV ACGT 1 cut(s) 319
HpyCH4V TGCA 4 cut(s) 479, 656, 998, 1320
HpyF10VI GCNNNNNNNGC 3 cut(s) 66, 87, 1356
HpyF3I CTNAG 6 cut(s) 244, 294, 417, 1003, 1256, 1373
HpySE526I ACGT 1 cut(s) 319
Hsp92II CATG 4 cut(s) 210, 625, 1013, 1309
HspAI GCGC 4 cut(s) 67, 88, 811, 881
KflI GGGWCCC 1 cut(s) 946
Kzo9I GATC 7 cut(s) 283, 517, 597, 673, 829, 1249, 1330
LguI GCTCTTC 1 cut(s) 975
LmnI GCTCC 3 cut(s) 433, 1013, 1180
Lsp1109I GCAGC 1 cut(s) 241
LweI GCATC 7 cut(s) 54, 364, 415, 466, 713, 892, 1172
MaeI CTAG 4 cut(s) 176, 387, 515, 1154
MaeII ACGT 1 cut(s) 319
MaeIII GTNAC 4 cut(s) 310, 553, 632, 745
MalI GATC 7 cut(s) 285, 519, 599, 675, 831, 1251, 1332
MboI GATC 7 cut(s) 283, 517, 597, 673, 829, 1249, 1330
MboII GAAGA 4 cut(s) 620, 962, 1088, 1136
MflI RGATCY 3 cut(s) 597, 829, 1330
MhlI GDGCHC 6 cut(s) 163, 375, 471, 963, 1018, 1293
MluCI AATT 7 cut(s) 363, 401, 490, 616, 780, 935, 985
MlyI GAGTC 2 cut(s) 773, 1090
MmeI TCCRAC 1 cut(s) 15
MroXI GAANNNNTTC 1 cut(s) 969
MseI TTAA 8 cut(s) 396, 489, 537, 594, 854, 1083, 1302, 1338
MslI CAYNNNNRTG 3 cut(s) 474, 692, 1304
MspA1I CMGCKG 1 cut(s) 770
MspI CCGG 2 cut(s) 61, 82
MvnI CGCG 2 cut(s) 69, 1248
MwoI GCNNNNNNNGC 3 cut(s) 66, 87, 1356
NdeII GATC 7 cut(s) 283, 517, 597, 673, 829, 1249, 1330
NlaIII CATG 4 cut(s) 210, 625, 1013, 1309
NlaIV GGNNCC 4 cut(s) 149, 161, 947, 948
NmeAIII GCCGAG 1 cut(s) 36
NmuCI GTSAC 1 cut(s) 632
NruI TCGCGA 1 cut(s) 1248
NspI RCATGY 1 cut(s) 1309
NspV TTCGAA 1 cut(s) 926
PciI ACATGT 1 cut(s) 1305
PciSI GCTCTTC 1 cut(s) 975
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PdmI GAANNNNTTC 1 cut(s) 969
PfeI GAWTC 7 cut(s) 44, 108, 130, 274, 380, 923, 1134
PkrI GCNGC 1 cut(s) 256
PleI GAGTC 2 cut(s) 772, 1090
PpsI GAGTC 2 cut(s) 772, 1090
PpuMI RGGWCCY 1 cut(s) 946
PscI ACATGT 1 cut(s) 1305
Psp124BI GAGCTC 1 cut(s) 1018
Psp1406I AACGTT 1 cut(s) 319
Psp5II RGGWCCY 1 cut(s) 946
PspN4I GGNNCC 4 cut(s) 149, 161, 947, 948
PspOMI GGGCCC 1 cut(s) 159
PspPI GGNCC 5 cut(s) 147, 159, 160, 844, 946
PspPPI RGGWCCY 1 cut(s) 946
PstNI CAGNNNCTG 1 cut(s) 144
PsuI RGATCY 3 cut(s) 597, 829, 1330
PvuII CAGCTG 1 cut(s) 770
RruI TCGCGA 1 cut(s) 1248
RsaI GTAC 6 cut(s) 143, 449, 757, 982, 993, 1272
RsaNI GTAC 6 cut(s) 142, 448, 756, 981, 992, 1271
RseI CAYNNNNRTG 3 cut(s) 474, 692, 1304
SacI GAGCTC 1 cut(s) 1018
SapI GCTCTTC 1 cut(s) 975
SaqAI TTAA 8 cut(s) 396, 489, 537, 594, 854, 1083, 1302, 1338
SatI GCNGC 1 cut(s) 255
Sau3AI GATC 7 cut(s) 283, 517, 597, 673, 829, 1249, 1330
Sau96I GGNCC 5 cut(s) 147, 159, 160, 844, 946
ScaI AGTACT 1 cut(s) 757
SchI GAGTC 2 cut(s) 773, 1090
SduI GDGCHC 6 cut(s) 163, 375, 471, 963, 1018, 1293
SfaNI GCATC 7 cut(s) 54, 364, 415, 466, 713, 892, 1172
SfcI CTRYAG 1 cut(s) 574
SfuI TTCGAA 1 cut(s) 926
SinI GGWCC 2 cut(s) 844, 946
SmiMI CAYNNNNRTG 3 cut(s) 474, 692, 1304
SmlI CTYRAG 4 cut(s) 350, 462, 759, 1292
SmoI CTYRAG 4 cut(s) 350, 462, 759, 1292
SpeI ACTAGT 1 cut(s) 386
Sse9I AATT 7 cut(s) 363, 401, 490, 616, 780, 935, 985
SsiI CCGC 5 cut(s) 257, 842, 1157, 1279, 1324
SspMI CTAG 4 cut(s) 176, 387, 515, 1154
SstI GAGCTC 1 cut(s) 1018
TaaI ACNGT 4 cut(s) 553, 566, 578, 751
TaiI ACGT 1 cut(s) 322
TaqI TCGA 2 cut(s) 926, 963
TasI AATT 7 cut(s) 363, 401, 490, 616, 780, 935, 985
TatI WGTACW 3 cut(s) 755, 991, 1270
TfiI GAWTC 7 cut(s) 44, 108, 130, 274, 380, 923, 1134
Tru1I TTAA 8 cut(s) 396, 489, 537, 594, 854, 1083, 1302, 1338
Tru9I TTAA 8 cut(s) 396, 489, 537, 594, 854, 1083, 1302, 1338
TscAI CASTG 4 cut(s) 36, 298, 583, 1197
TseFI GTSAC 1 cut(s) 632
TseI GCWGC 1 cut(s) 254
Tsp45I GTSAC 1 cut(s) 632
TspDTI ATGAA 4 cut(s) 561, 621, 638, 756
TspGWI ACGGA 2 cut(s) 132, 420
TspRI CASTG 4 cut(s) 36, 298, 583, 1197
VpaK11BI GGWCC 2 cut(s) 844, 946
XagI CCTNNNNNAGG 1 cut(s) 713
XapI RAATTY 2 cut(s) 363, 985
XbaI TCTAGA 1 cut(s) 514
XceI RCATGY 1 cut(s) 1309
XcmI CCANNNNNNNNNTGG 1 cut(s) 854
XmnI GAANNNNTTC 1 cut(s) 969
XspI CTAG 4 cut(s) 176, 387, 515, 1154
ZrmI AGTACT 1 cut(s) 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.