Rroxscaffold_4G00329640

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002998
Physical Location & Seq
Forward (+)
62934449 .. 62936416
1968 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00329640.1

Sequence Viewer

Length: 984 bp
ATGTTCAACAGCAACAGCGTATCAATCTCAGTCTCCGATGACGAATCCGACGAGCTTGGCCGGATGCGCGTCCGAGTTCGCCGGAAGCGCAAGAAACTCGGCCACCGATTCAAGAACGAGTTCGTTCGCCGAGTGGTGAGGAATCTGGTCAAGTACTGGGCCCTTCTGATTCTTCTCCCAGCCATTGGTTTGCTTCTGTATGAGGCTTGGAGTATCGGCTGGAAGTCCCCACAGCTCAATAATAAGTCGGAAAAGCCGAGTTTGGTCCACTCGGAACTCAATGCGAGTTCGGAGAAGAAATCCGATGGCAATTTGAACCGGCTTGATCCTATTACTCGGGTTATTCATGGCGTTAGGCAACGTTGCTTGCAAATCTTACCTCCTGAAGAACTTGAGCATCTGGAAATTCATGTGCGCGAAGATGCCACTAGTCCTGTTAAGAAATTGTTATACAGAACGGAGAATGATGGTCCGTTGTTAAGAGGGTACAACACCCGGTCTGAAGAGCACACAGAGGCCACGAGATTTAATTTGTTTACGGGAAATCAAACTCTAGATCAGAGAGGTGAAATGTTTAAGGTAAATGAAACTGTTACCATAAACTGTGGTTTCTACAGTGAAAATGGTGGGTTTAAGATCTCAAATGAAGACAAGAATTACATGCAAAGTTGTGACATTGTAGTCTCCACTTGTGCATTCGGTGGTGGAGATGATCTTTATCAACCCATTGGAATGTCAGAGGCATCTCTTCAGAAGGTTTGCTATGTTGCTTTTTGGGATGAAGTTACTGTAAGTACTCAGGAATCAGCTGAACATAGAATTGGTGAGTATGGATTTATTGGGAAATGGCGCATTGTGGTTGTAAGCGATCTGCCTTTTGCGGACCAAAGGTTAAATGGAAAAATTCCAAAGGGAGCATACCCCGTTGACGAATATGTTTACGTGCCTCTGGTTCAACGTGGTCGTTCGTTTCACGTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616 Dirigent

Protein Analysis

327

Amino Acids

37.4

Weight (kDa)

8.18

Isoelectric Point (pI)

39.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 167 - 304 1.5e-45 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 680
AccB7I CCANNNNNTGG 1 cut(s) 185
AccII CGCG 2 cut(s) 69, 417
AciI CCGC 1 cut(s) 881
AclI AACGTT 1 cut(s) 361
AclWI GGATC 1 cut(s) 320
AcoI YGGCCR 2 cut(s) 58, 100
AcsI RAATTY 2 cut(s) 405, 903
AcuI CTGAAG 3 cut(s) 405, 522, 734
AfaI GTAC 3 cut(s) 155, 488, 796
AfiI CCNNNNNNNGG 1 cut(s) 185
AgsI TTSAA 4 cut(s) 7, 112, 316, 956
AhlI ACTAGT 1 cut(s) 428
AjiI CACGTC 1 cut(s) 976
AjuI GAANNNNNNNTTGG 2 cut(s) 804, 836
AluBI AGCT 3 cut(s) 55, 235, 809
AluI AGCT 3 cut(s) 55, 235, 809
Alw21I GWGCWC 1 cut(s) 510
Alw26I GTCTC 2 cut(s) 37, 688
AlwI GGATC 1 cut(s) 320
Ama87I CYCGRG 1 cut(s) 336
AoxI GGCC 4 cut(s) 58, 100, 159, 516
ApaI GGGCCC 1 cut(s) 163
ApoI RAATTY 2 cut(s) 405, 903
Asp700I GAANNNNTTC 1 cut(s) 119
AspLEI GCGC 4 cut(s) 69, 90, 417, 852
AspS9I GGNCC 5 cut(s) 159, 160, 265, 470, 883
AsuC2I CCSGG 1 cut(s) 496
AsuHPI GGTGA 3 cut(s) 148, 578, 836
AvaI CYCGRG 1 cut(s) 336
AvaII GGWCC 3 cut(s) 265, 470, 883
BaeGI GKGCMC 1 cut(s) 163
BanII GRGCYC 1 cut(s) 163
BauI CACGAG 1 cut(s) 520
BbsI GAAGAC 1 cut(s) 654
Bbv12I GWGCWC 1 cut(s) 510
BccI CCATC 2 cut(s) 299, 461
BcgI CGANNNNNNTGC 2 cut(s) 79, 113
BcnI CCSGG 1 cut(s) 496
BcoDI GTCTC 2 cut(s) 37, 688
BcuI ACTAGT 1 cut(s) 428
BfaI CTAG 2 cut(s) 429, 554
BfmI CTRYAG 1 cut(s) 613
BglII AGATCT 1 cut(s) 636
BmcAI AGTACT 2 cut(s) 155, 796
Bme1390I CCNGG 1 cut(s) 496
Bme18I GGWCC 3 cut(s) 265, 470, 883
BmeT110I CYCGRG 1 cut(s) 336
BmgBI CACGTC 1 cut(s) 976
BmgT120I GGNCC 5 cut(s) 159, 160, 265, 470, 883
BmiI GGNNCC 1 cut(s) 161
BmrFI CCNGG 1 cut(s) 496
BmrI ACTGGG 1 cut(s) 166
BmsI GCATC 4 cut(s) 54, 406, 412, 752
BmuI ACTGGG 1 cut(s) 166
BpiI GAAGAC 1 cut(s) 654
BpuEI CTTGAG 1 cut(s) 413
BpuMI CCSGG 1 cut(s) 496
BsaAI YACGTR 1 cut(s) 943
BsaBI GATNNNNATC 1 cut(s) 717
Bsc4I CCNNNNNNNGG 1 cut(s) 185
Bse118I RCCGGY 1 cut(s) 318
Bse1I ACTGG 1 cut(s) 161
Bse8I GATNNNNATC 1 cut(s) 717
BseGI GGATG 2 cut(s) 69, 784
BseJI GATNNNNATC 1 cut(s) 717
BseLI CCNNNNNNNGG 1 cut(s) 185
BseMII CTCAG 2 cut(s) 42, 812
BseNI ACTGG 1 cut(s) 161
BseSI GKGCMC 1 cut(s) 163
BseYI CCCAGC 1 cut(s) 178
Bsh1236I CGCG 2 cut(s) 69, 417
BshFI GGCC 4 cut(s) 60, 102, 161, 518
BsiHKAI GWGCWC 1 cut(s) 510
BsiHKCI CYCGRG 1 cut(s) 336
BsiSI CCGG 4 cut(s) 61, 82, 319, 496
BslFI GGGAC 1 cut(s) 211
BslI CCNNNNNNNGG 1 cut(s) 185
BsmAI GTCTC 2 cut(s) 37, 688
BsmFI GGGAC 1 cut(s) 211
BsmI GAATGC 1 cut(s) 695
BsnI GGCC 4 cut(s) 60, 102, 161, 518
BsoBI CYCGRG 1 cut(s) 336
Bsp120I GGGCCC 1 cut(s) 159
Bsp1286I GDGCHC 2 cut(s) 163, 510
Bsp143I GATC 5 cut(s) 325, 556, 636, 712, 868
BspACI CCGC 1 cut(s) 881
BspANI GGCC 4 cut(s) 60, 102, 161, 518
BspCNI CTCAG 2 cut(s) 41, 811
BspFNI CGCG 2 cut(s) 69, 417
BspLI GGNNCC 1 cut(s) 161
BspPI GGATC 1 cut(s) 320
BspQI GCTCTTC 1 cut(s) 498
BsrFI RCCGGY 1 cut(s) 318
BsrI ACTGG 1 cut(s) 161
BssAI RCCGGY 1 cut(s) 318
BssMI GATC 5 cut(s) 325, 556, 636, 712, 868
BssSI CACGAG 1 cut(s) 520
Bst2BI CACGAG 1 cut(s) 520
Bst4CI ACNGT 4 cut(s) 592, 605, 617, 790
Bst6I CTCTTC 2 cut(s) 498, 753
BstBAI YACGTR 1 cut(s) 943
BstC8I GCNNGC 1 cut(s) 368
BstDEI CTNAG 2 cut(s) 28, 798
BstF5I GGATG 2 cut(s) 69, 784
BstFNI CGCG 2 cut(s) 69, 417
BstHHI GCGC 4 cut(s) 69, 90, 417, 852
BstKTI GATC 5 cut(s) 328, 559, 639, 715, 871
BstMAI GTCTC 2 cut(s) 37, 688
BstMBI GATC 5 cut(s) 325, 556, 636, 712, 868
BstMWI GCNNNNNNNGC 2 cut(s) 66, 87
BstNSI RCATGY 1 cut(s) 664
BstSCI CCNGG 1 cut(s) 494
BstSFI CTRYAG 1 cut(s) 613
BstSLI GKGCMC 1 cut(s) 163
BstUI CGCG 2 cut(s) 69, 417
BstV2I GAAGAC 1 cut(s) 654
BstX2I RGATCY 1 cut(s) 636
BstYI RGATCY 1 cut(s) 636
BsuRI GGCC 4 cut(s) 60, 102, 161, 518
BtrI CACGTC 1 cut(s) 976
BtsCI GGATG 2 cut(s) 69, 784
BtsIMutI CAGTG 1 cut(s) 622
Cac8I GCNNGC 1 cut(s) 368
CfoI GCGC 4 cut(s) 69, 90, 417, 852
Cfr10I RCCGGY 1 cut(s) 318
Cfr13I GGNCC 5 cut(s) 159, 160, 265, 470, 883
CseI GACGC 1 cut(s) 58
Csp6I GTAC 3 cut(s) 154, 487, 795
CviAII CATG 3 cut(s) 347, 410, 661
CviQI GTAC 3 cut(s) 154, 487, 795
DdeI CTNAG 2 cut(s) 28, 798
DpnI GATC 5 cut(s) 327, 558, 638, 714, 870
DpnII GATC 5 cut(s) 325, 556, 636, 712, 868
DrdI GACNNNNNNGTC 1 cut(s) 680
DseDI GACNNNNNNGTC 1 cut(s) 680
EaeI YGGCCR 2 cut(s) 58, 100
Eam1104I CTCTTC 2 cut(s) 498, 753
EarI CTCTTC 2 cut(s) 498, 753
Eco24I GRGCYC 1 cut(s) 163
Eco47I GGWCC 3 cut(s) 265, 470, 883
Eco57I CTGAAG 3 cut(s) 405, 522, 734
Eco88I CYCGRG 1 cut(s) 336
EcoO109I RGGNCCY 1 cut(s) 160
EcoT38I GRGCYC 1 cut(s) 163
FaeI CATG 3 cut(s) 350, 413, 664
FaqI GGGAC 1 cut(s) 211
FatI CATG 3 cut(s) 346, 409, 660
FokI GGATG 2 cut(s) 76, 791
FriOI GRGCYC 1 cut(s) 163
FspBI CTAG 2 cut(s) 429, 554
GlaI GCGC 4 cut(s) 68, 89, 416, 851
GsaI CCCAGC 1 cut(s) 182
HaeIII GGCC 4 cut(s) 60, 102, 161, 518
HapII CCGG 4 cut(s) 61, 82, 319, 496
HgaI GACGC 1 cut(s) 58
HhaI GCGC 4 cut(s) 69, 90, 417, 852
Hin1II CATG 3 cut(s) 350, 413, 664
Hin6I GCGC 4 cut(s) 67, 88, 415, 850
HinP1I GCGC 4 cut(s) 67, 88, 415, 850
HincII GTYRAC 1 cut(s) 928
HindII GTYRAC 1 cut(s) 928
HinfI GANTC 5 cut(s) 44, 108, 142, 169, 803
HpaII CCGG 4 cut(s) 61, 82, 319, 496
HphI GGTGA 3 cut(s) 148, 578, 836
Hpy166II GTNNAC 4 cut(s) 268, 537, 928, 940
Hpy188III TCNNGA 5 cut(s) 112, 383, 401, 554, 800
Hpy8I GTNNAC 4 cut(s) 268, 537, 928, 940
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 2 cut(s) 173, 748
HpyCH4III ACNGT 4 cut(s) 592, 605, 617, 790
HpyCH4IV ACGT 4 cut(s) 361, 942, 958, 975
HpyCH4V TGCA 3 cut(s) 370, 664, 695
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 87
HpyF3I CTNAG 2 cut(s) 28, 798
HpySE526I ACGT 4 cut(s) 361, 942, 958, 975
Hsp92II CATG 3 cut(s) 350, 413, 664
HspAI GCGC 4 cut(s) 67, 88, 415, 850
Kzo9I GATC 5 cut(s) 325, 556, 636, 712, 868
LguI GCTCTTC 1 cut(s) 498
LmnI GCTCC 1 cut(s) 914
LweI GCATC 4 cut(s) 54, 406, 412, 752
MaeI CTAG 2 cut(s) 429, 554
MaeII ACGT 4 cut(s) 361, 942, 958, 975
MaeIII GTNAC 3 cut(s) 592, 671, 784
MalI GATC 5 cut(s) 327, 558, 638, 714, 870
MboI GATC 5 cut(s) 325, 556, 636, 712, 868
MboII GAAGA 7 cut(s) 164, 307, 398, 431, 515, 659, 740
MflI RGATCY 1 cut(s) 636
MhlI GDGCHC 2 cut(s) 163, 510
MluCI AATT 7 cut(s) 310, 405, 443, 529, 655, 819, 903
MmeI TCCRAC 2 cut(s) 72, 228
MnlI CCTC 8 cut(s) 132, 196, 390, 476, 508, 557, 733, 957
MroXI GAANNNNTTC 1 cut(s) 119
MseI TTAA 6 cut(s) 438, 479, 528, 576, 633, 893
MslI CAYNNNNRTG 1 cut(s) 731
MspA1I CMGCKG 1 cut(s) 809
MspI CCGG 4 cut(s) 61, 82, 319, 496
MspR9I CCNGG 1 cut(s) 496
Mva1269I GAATGC 1 cut(s) 695
MvnI CGCG 2 cut(s) 69, 417
MwoI GCNNNNNNNGC 2 cut(s) 66, 87
NciI CCSGG 1 cut(s) 496
NdeII GATC 5 cut(s) 325, 556, 636, 712, 868
NlaIII CATG 3 cut(s) 350, 413, 664
NlaIV GGNNCC 1 cut(s) 161
NmeAIII GCCGAG 3 cut(s) 78, 155, 282
NmuCI GTSAC 1 cut(s) 671
NspI RCATGY 1 cut(s) 664
PciSI GCTCTTC 1 cut(s) 498
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 254
PctI GAATGC 1 cut(s) 695
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 5 cut(s) 44, 108, 142, 169, 803
PflMI CCANNNNNTGG 1 cut(s) 185
Ppu21I YACGTR 1 cut(s) 943
Psp1406I AACGTT 1 cut(s) 361
PspFI CCCAGC 1 cut(s) 178
PspN4I GGNNCC 1 cut(s) 161
PspOMI GGGCCC 1 cut(s) 159
PspPI GGNCC 5 cut(s) 159, 160, 265, 470, 883
PsuI RGATCY 1 cut(s) 636
PvuII CAGCTG 1 cut(s) 809
RsaI GTAC 3 cut(s) 155, 488, 796
RsaNI GTAC 3 cut(s) 154, 487, 795
RseI CAYNNNNRTG 1 cut(s) 731
SapI GCTCTTC 1 cut(s) 498
SaqAI TTAA 6 cut(s) 438, 479, 528, 576, 633, 893
Sau3AI GATC 5 cut(s) 325, 556, 636, 712, 868
Sau96I GGNCC 5 cut(s) 159, 160, 265, 470, 883
ScaI AGTACT 2 cut(s) 155, 796
ScrFI CCNGG 1 cut(s) 496
SduI GDGCHC 2 cut(s) 163, 510
SfaNI GCATC 4 cut(s) 54, 406, 412, 752
SfcI CTRYAG 1 cut(s) 613
SinI GGWCC 3 cut(s) 265, 470, 883
SmiMI CAYNNNNRTG 1 cut(s) 731
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
SpeI ACTAGT 1 cut(s) 428
Sse9I AATT 7 cut(s) 310, 405, 443, 529, 655, 819, 903
SsiI CCGC 1 cut(s) 881
SspMI CTAG 2 cut(s) 429, 554
StyD4I CCNGG 1 cut(s) 494
TaaI ACNGT 4 cut(s) 592, 605, 617, 790
TaiI ACGT 4 cut(s) 364, 945, 961, 978
TasI AATT 7 cut(s) 310, 405, 443, 529, 655, 819, 903
TatI WGTACW 2 cut(s) 153, 794
TfiI GAWTC 5 cut(s) 44, 108, 142, 169, 803
Tru1I TTAA 6 cut(s) 438, 479, 528, 576, 633, 893
Tru9I TTAA 6 cut(s) 438, 479, 528, 576, 633, 893
TscAI CASTG 1 cut(s) 622
TseFI GTSAC 1 cut(s) 671
Tsp45I GTSAC 1 cut(s) 671
TspDTI ATGAA 5 cut(s) 335, 398, 600, 660, 795
TspGWI ACGGA 2 cut(s) 462, 473
TspRI CASTG 1 cut(s) 622
Van91I CCANNNNNTGG 1 cut(s) 185
VpaK11BI GGWCC 3 cut(s) 265, 470, 883
XapI RAATTY 2 cut(s) 405, 903
XbaI TCTAGA 1 cut(s) 553
XceI RCATGY 1 cut(s) 664
XcmI CCANNNNNNNNNTGG 1 cut(s) 893
XmnI GAANNNNTTC 1 cut(s) 119
XspI CTAG 2 cut(s) 429, 554
ZrmI AGTACT 2 cut(s) 155, 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.