Rorug01G0020100

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002998
Physical Location & Seq
Forward (+)
3290764 .. 3296264
5501 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0020100.1

Sequence Viewer

Length: 975 bp
ATGTATGAGAAGTATCATGTCTGTTATGGAGGTGAAGAGGAAGCGAGAAAATCAAACTATACTGATATGGTTAATAAGTATTATGATCTGGTTACCAGCTTTTATGAGTATGGCTGGGGAGAGTCCTTCCATTTTGCACCCAGATGGAAAGGAGAGTCTCTTCGAGAGAGCATCAAGAGGCATGAACACTTCCTTGCCTTACAACTAGGACTGAAACCTGGACAGAAGGTTTTGGATGTAGGTTGTGGAATTGGAGGACCACTTAGAGAAATAGCACGCTTCAGCTCGACGTCAATTACTGGGTTGAACAACAATGAATATCAGATAACAAGAGGAAAGGAATTGAACCGCATTGTTGGAATTGACAAGACCTGTGACTTTGTTAAGGCCGACTTCATGAAAATACCATTTCCTGAAAATACGTTTGATGCAGTATATGCAATCGAAGCTACCTGCCATGCACCAGATGCATATGGATGTTACAAAGAGATTTATAGAGTACTAAAACCTGGTCAATGTTTTGCTGCATATGAGTGGTGCATGACTGATTCGTTTGATCCCAATAACGCAGAACATCAAAAAATTAAGGCAGAGATTGAGATTGGTGATGGCCTCCCGGACATCAGATTGACAGTAAAGTGCCTTGAAGCTTTGAAACAAGCAGGTTTTGAGGTCATATGGGAGAAAGATCTTGCAAAGGACGTAAAGGAGTCACCTCTTCCTTGGTACTTGCCTTTGGATAAAAGTCGCATTTCACTTAGTAGTTTCCGTCTAACAGCTGTTGGCCGCTTTTTTACTAAAAACATGGTCAAAGTTTTAGAATATGTTGGACTTGCTCCACAAGGAAGCCAAAGAGTTCAAGATTTTCTAGAGAAAGCTGCTGAAGGGCTAGTCGAAGGTGGAAAGAAAGAGATCTTCACACCAATGTATTTCTTTGTGGCCCGGAAGCCACTTTCAGCTGCAGATAGTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616 Dirigent

Protein Analysis

324

Amino Acids

36.88

Weight (kDa)

6.14

Isoelectric Point (pI)

34.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 18 - 249 8.4e-11 Mycolic acid cyclopropane synthetase
Ubie_methyltran PF01209 64 - 179 1.1e-09 ubiE/COQ5 methyltransferase family
Methyltransf_23 PF13489 66 - 172 3.6e-08 Methyltransferase domain
Methyltransf_31 PF13847 72 - 191 5.9e-15 Methyltransferase domain
Methyltransf_25 PF13649 77 - 171 1.8e-17 Methyltransferase domain
Methyltransf_11 PF08241 78 - 173 1.1e-20 Methyltransferase domain
Methyltransf_12 PF08242 78 - 171 2.7e-08 Methyltransferase domain
Sterol_MT_C PF08498 254 - 317 6e-31 Sterol methyltransferase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 293
Acc36I ACCTGC 2 cut(s) 461, 653
AciI CCGC 2 cut(s) 349, 787
AclWI GGATC 1 cut(s) 551
AcoI YGGCCR 1 cut(s) 784
AcuI CTGAAG 2 cut(s) 265, 903
AcyI GRCGYC 1 cut(s) 290
AfaI GTAC 2 cut(s) 501, 728
AgsI TTSAA 5 cut(s) 307, 346, 647, 655, 860
AjnI CCWGG 2 cut(s) 217, 508
AluBI AGCT 7 cut(s) 99, 285, 449, 650, 779, 878, 959
AluI AGCT 7 cut(s) 99, 285, 449, 650, 779, 878, 959
Alw26I GTCTC 1 cut(s) 162
AlwI GGATC 1 cut(s) 551
AoxI GGCC 4 cut(s) 387, 610, 784, 939
ApeKI GCWGC 3 cut(s) 524, 878, 959
AspS9I GGNCC 2 cut(s) 257, 940
AsuC2I CCSGG 2 cut(s) 617, 943
AsuHPI GGTGA 3 cut(s) 44, 617, 705
AvaII GGWCC 1 cut(s) 257
BbvI GCAGC 3 cut(s) 511, 865, 946
BccI CCATC 2 cut(s) 138, 602
BciT130I CCWGG 2 cut(s) 219, 510
BcnI CCSGG 2 cut(s) 617, 943
BcoDI GTCTC 1 cut(s) 162
BfaI CTAG 3 cut(s) 206, 869, 890
BfmI CTRYAG 1 cut(s) 960
BfuAI ACCTGC 2 cut(s) 461, 653
BglII AGATCT 2 cut(s) 688, 912
BisI GCNGC 4 cut(s) 525, 787, 879, 960
BlsI GCNGC 4 cut(s) 526, 788, 880, 961
BmcAI AGTACT 1 cut(s) 501
Bme1390I CCNGG 4 cut(s) 219, 510, 617, 943
Bme18I GGWCC 1 cut(s) 257
BmgT120I GGNCC 2 cut(s) 257, 940
BmrFI CCNGG 4 cut(s) 219, 510, 617, 943
BmrI ACTGGG 1 cut(s) 309
BmsI GCATC 3 cut(s) 180, 418, 457
BmuI ACTGGG 1 cut(s) 309
BpuMI CCSGG 2 cut(s) 617, 943
BsaHI GRCGYC 1 cut(s) 290
BsaJI CCNNGG 1 cut(s) 722
Bse1I ACTGG 1 cut(s) 304
BseBI CCWGG 2 cut(s) 219, 510
BseDI CCNNGG 1 cut(s) 722
BseGI GGATG 2 cut(s) 241, 482
BseNI ACTGG 1 cut(s) 304
BseXI GCAGC 3 cut(s) 511, 865, 946
BseYI CCCAGC 1 cut(s) 114
BshFI GGCC 4 cut(s) 389, 612, 786, 941
BsiSI CCGG 2 cut(s) 617, 943
BsmAI GTCTC 1 cut(s) 162
BsnI GGCC 4 cut(s) 389, 612, 786, 941
Bsp143I GATC 4 cut(s) 85, 556, 688, 912
BspACI CCGC 2 cut(s) 349, 787
BspANI GGCC 4 cut(s) 389, 612, 786, 941
BspHI TCATGA 1 cut(s) 396
BspMAI CTGCAG 1 cut(s) 964
BspMI ACCTGC 2 cut(s) 461, 653
BspPI GGATC 1 cut(s) 551
BsrI ACTGG 1 cut(s) 304
BssECI CCNNGG 1 cut(s) 722
BssMI GATC 4 cut(s) 85, 556, 688, 912
BssNI GRCGYC 1 cut(s) 290
BssT1I CCWWGG 1 cut(s) 722
Bst2UI CCWGG 2 cut(s) 219, 510
Bst4CI ACNGT 1 cut(s) 634
Bst6I CTCTTC 3 cut(s) 30, 165, 723
BstACI GRCGYC 1 cut(s) 290
BstAPI GCANNNNNTGC 2 cut(s) 437, 467
BstC8I GCNNGC 1 cut(s) 277
BstDEI CTNAG 2 cut(s) 263, 758
BstEII GGTNACC 1 cut(s) 91
BstF5I GGATG 2 cut(s) 241, 482
BstKTI GATC 4 cut(s) 88, 559, 691, 915
BstMAI GTCTC 1 cut(s) 162
BstMBI GATC 4 cut(s) 85, 556, 688, 912
BstMWI GCNNNNNNNGC 3 cut(s) 437, 446, 467
BstNI CCWGG 2 cut(s) 219, 510
BstPI GGTNACC 1 cut(s) 91
BstSCI CCNGG 4 cut(s) 217, 508, 615, 941
BstSFI CTRYAG 1 cut(s) 960
BstV1I GCAGC 3 cut(s) 511, 865, 946
BstX2I RGATCY 2 cut(s) 688, 912
BstYI RGATCY 2 cut(s) 688, 912
BsuRI GGCC 4 cut(s) 389, 612, 786, 941
BtsCI GGATG 2 cut(s) 241, 482
BveI ACCTGC 2 cut(s) 461, 653
Cac8I GCNNGC 1 cut(s) 277
CciI TCATGA 1 cut(s) 396
Cfr13I GGNCC 2 cut(s) 257, 940
CsiI ACCWGGT 1 cut(s) 508
Csp6I GTAC 2 cut(s) 500, 727
CviAII CATG 6 cut(s) 17, 182, 397, 458, 541, 805
CviQI GTAC 2 cut(s) 500, 727
DdeI CTNAG 2 cut(s) 263, 758
DpnI GATC 4 cut(s) 87, 558, 690, 914
DpnII GATC 4 cut(s) 85, 556, 688, 912
EaeI YGGCCR 1 cut(s) 784
Eam1104I CTCTTC 3 cut(s) 30, 165, 723
EarI CTCTTC 3 cut(s) 30, 165, 723
Eco130I CCWWGG 1 cut(s) 722
Eco47I GGWCC 1 cut(s) 257
Eco57I CTGAAG 2 cut(s) 265, 903
Eco91I GGTNACC 1 cut(s) 91
EcoO65I GGTNACC 1 cut(s) 91
EcoRII CCWGG 2 cut(s) 217, 508
EcoT14I CCWWGG 1 cut(s) 722
EcoT22I ATGCAT 1 cut(s) 472
ErhI CCWWGG 1 cut(s) 722
FaeI CATG 6 cut(s) 20, 185, 400, 461, 544, 808
FalI AAGNNNNNCTT 2 cut(s) 377, 409
FatI CATG 6 cut(s) 16, 181, 396, 457, 540, 804
FauNDI CATATG 3 cut(s) 472, 529, 677
Fnu4HI GCNGC 4 cut(s) 525, 787, 879, 960
FokI GGATG 2 cut(s) 248, 489
Fsp4HI GCNGC 4 cut(s) 525, 787, 879, 960
FspBI CTAG 3 cut(s) 206, 869, 890
GluI GCNGC 4 cut(s) 525, 787, 879, 960
GsaI CCCAGC 1 cut(s) 118
HaeIII GGCC 4 cut(s) 389, 612, 786, 941
HapII CCGG 2 cut(s) 617, 943
Hin1I GRCGYC 1 cut(s) 290
Hin1II CATG 6 cut(s) 20, 185, 400, 461, 544, 808
HindIII AAGCTT 1 cut(s) 648
HinfI GANTC 4 cut(s) 122, 155, 548, 710
HpaII CCGG 2 cut(s) 617, 943
HphI GGTGA 3 cut(s) 44, 617, 705
Hpy188I TCNGA 2 cut(s) 324, 626
Hpy188III TCNNGA 6 cut(s) 164, 175, 397, 413, 860, 869
Hpy99I CGWCG 1 cut(s) 292
HpyAV CCTTC 4 cut(s) 136, 220, 878, 890
HpyCH4III ACNGT 1 cut(s) 634
HpyCH4IV ACGT 3 cut(s) 290, 422, 702
HpyCH4V TGCA 9 cut(s) 137, 431, 440, 461, 470, 527, 540, 695, 962
HpyF10VI GCNNNNNNNGC 3 cut(s) 437, 446, 467
HpyF3I CTNAG 2 cut(s) 263, 758
HpySE526I ACGT 3 cut(s) 290, 422, 702
Hsp92I GRCGYC 1 cut(s) 290
Hsp92II CATG 6 cut(s) 20, 185, 400, 461, 544, 808
Kzo9I GATC 4 cut(s) 85, 556, 688, 912
LmnI GCTCC 1 cut(s) 841
Lsp1109I GCAGC 3 cut(s) 511, 865, 946
LweI GCATC 3 cut(s) 180, 418, 457
MabI ACCWGGT 1 cut(s) 508
MaeI CTAG 3 cut(s) 206, 869, 890
MaeII ACGT 3 cut(s) 290, 422, 702
MaeIII GTNAC 4 cut(s) 91, 374, 479, 711
MalI GATC 4 cut(s) 87, 558, 690, 914
MboI GATC 4 cut(s) 85, 556, 688, 912
MboII GAAGA 4 cut(s) 47, 152, 710, 907
MflI RGATCY 2 cut(s) 688, 912
MluCI AATT 5 cut(s) 249, 294, 341, 360, 582
MlyI GAGTC 3 cut(s) 131, 164, 719
MmeI TCCRAC 2 cut(s) 337, 808
MnlI CCTC 8 cut(s) 23, 31, 171, 248, 326, 623, 664, 726
Mph1103I ATGCAT 1 cut(s) 472
MseI TTAA 3 cut(s) 72, 384, 585
MslI CAYNNNNRTG 4 cut(s) 142, 475, 532, 923
MspA1I CMGCKG 2 cut(s) 779, 959
MspI CCGG 2 cut(s) 617, 943
MspR9I CCNGG 4 cut(s) 219, 510, 617, 943
MvaI CCWGG 2 cut(s) 219, 510
MwoI GCNNNNNNNGC 3 cut(s) 437, 446, 467
NciI CCSGG 2 cut(s) 617, 943
NdeI CATATG 3 cut(s) 472, 529, 677
NdeII GATC 4 cut(s) 85, 556, 688, 912
NlaIII CATG 6 cut(s) 20, 185, 400, 461, 544, 808
NmuCI GTSAC 2 cut(s) 374, 711
NsiI ATGCAT 1 cut(s) 472
PagI TCATGA 1 cut(s) 396
PfeI GAWTC 1 cut(s) 548
PfoI TCCNGGA 1 cut(s) 615
PkrI GCNGC 4 cut(s) 526, 788, 880, 961
PleI GAGTC 3 cut(s) 130, 163, 718
PpsI GAGTC 3 cut(s) 130, 163, 718
Psp6I CCWGG 2 cut(s) 217, 508
PspEI GGTNACC 1 cut(s) 91
PspFI CCCAGC 1 cut(s) 114
PspGI CCWGG 2 cut(s) 217, 508
PspPI GGNCC 2 cut(s) 257, 940
PstI CTGCAG 1 cut(s) 964
PsuI RGATCY 2 cut(s) 688, 912
PvuII CAGCTG 2 cut(s) 779, 959
RsaI GTAC 2 cut(s) 501, 728
RsaNI GTAC 2 cut(s) 500, 727
RseI CAYNNNNRTG 4 cut(s) 142, 475, 532, 923
SaqAI TTAA 3 cut(s) 72, 384, 585
SatI GCNGC 4 cut(s) 525, 787, 879, 960
Sau3AI GATC 4 cut(s) 85, 556, 688, 912
Sau96I GGNCC 2 cut(s) 257, 940
ScaI AGTACT 1 cut(s) 501
SchI GAGTC 3 cut(s) 131, 164, 719
ScrFI CCNGG 4 cut(s) 219, 510, 617, 943
SexAI ACCWGGT 1 cut(s) 508
SfaNI GCATC 3 cut(s) 180, 418, 457
SfcI CTRYAG 1 cut(s) 960
SinI GGWCC 1 cut(s) 257
SmiMI CAYNNNNRTG 4 cut(s) 142, 475, 532, 923
Sse9I AATT 5 cut(s) 249, 294, 341, 360, 582
SsiI CCGC 2 cut(s) 349, 787
SspMI CTAG 3 cut(s) 206, 869, 890
StyD4I CCNGG 4 cut(s) 217, 508, 615, 941
StyI CCWWGG 1 cut(s) 722
TaaI ACNGT 1 cut(s) 634
TaiI ACGT 3 cut(s) 293, 425, 705
TaqI TCGA 4 cut(s) 163, 287, 444, 894
TasI AATT 5 cut(s) 249, 294, 341, 360, 582
TatI WGTACW 1 cut(s) 499
TauI GCSGC 1 cut(s) 789
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 3 cut(s) 72, 384, 585
Tru9I TTAA 3 cut(s) 72, 384, 585
TseFI GTSAC 2 cut(s) 374, 711
TseI GCWGC 3 cut(s) 524, 878, 959
Tsp45I GTSAC 2 cut(s) 374, 711
TspDTI ATGAA 4 cut(s) 198, 330, 385, 413
TspGWI ACGGA 1 cut(s) 758
VpaK11BI GGWCC 1 cut(s) 257
XbaI TCTAGA 1 cut(s) 868
XspI CTAG 3 cut(s) 206, 869, 890
ZraI GACGTC 1 cut(s) 291
ZrmI AGTACT 1 cut(s) 501
Zsp2I ATGCAT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.