MD07G1022800.v1.1

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002998
Physical Location & Seq
Reverse (-)
1891023 .. 1894925
3903 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1022800.v1.1.491

Sequence Viewer

Length: 1440 bp
ATGTTCAGCAACAACAGCATATCAATCTCAGTCTCCGACGACGAAGCGGACGAACTCGGCCGGATGCGAGTCCGAGTTCGAAGAAAGCGCAAGAAACTCGGCCACCGACTCAAGAACGAGTTCCTCCACCGAGTCGCTAGAAAGCTCGTCAGGTACTGGGCACTTCTCATTTTTCTCCCGGCCCTTGGGTTGCTCTTATACGAGGTTTCGAGGATCGGTAGGAAGCCGAGTTTGGTGGCGAAGTCGGAACTCAGTGAGCCGGAAAAGCCGAGTTTGGTTGGCTCGGAAACGGTGAAGAACTCGAGTTTGGAGAAGAAATCGGATGGGAATTTGAATCGGCTCGATCATACGACTCGGGTTGTTGGAGGCGTTAGACAACGTTGTTTGGACATCTTATCTCCTGAAGAACTTGAGCATCTGGAAATTCCTATGCGCGAAGATTCCAATACACCTGTTAAGAAAGTGGTATACATATCAGAGAATGACAATCCGTTTTCAGGAGGGAACAACAGTCTTTCTCAGGAGCACACAGAGGCCCCAAGATTTAATTCATTTACTGGAAGTCAAACTCTGGATCAGAGAGCAAAATCGTTTAAGGTAAATGAAACTGTTACTATGAACTGTGGTTTCTACAGTGAAAATGGAGGGTTTAAAATCTCAAATGAAGACAAGGGGTACATGGATAGTTGCAAGGTTGTAGTGTCCACATGTGCATTTGGTGGTGGAGATGATCTTTATCAGCCTATTGGAATGTCAGAGGCATCACTTCGAAAGGTTTGCTATGTTGCTTTTTGGGATGAAATTACTCTAAGTACACAGGAAGCGGCTGAACATAGAATTGATGGGAATGGATTTATTGGGAAATGGCGCATTGTGGTTGTTAAAGATCTTCCCTTTTCAGACCAAAGGTTAAATGGAAAGATCCCAAAGATGTTGCCCCATCGTCTTTTTCCTCATGCAAAGTATTCCATTTGGGTAGATTCAAAGTCCCAATTTAGGAGGGACCCCTTAGGTGTGCTCGAAGCCCTACTTTGGCGTTCAAACTCTGTACTTGCTATCTCAGAACATGGAGCTCGCAGTAGCGTTTATGATGAAGCGAAGGCTGTTGTGAAAAAAAACAAGGCCAAACCAGAAGAAGTTGAGGTGCAATTGACTCAATACCGCCATGATGGCTTGCCAGAAGACAAGAGGTTCAACGGAAAGAAGGCTTTATCTGAAGCATCTGTTATCGTGAGGGAGCATACACCATTGACTAATATGTTTATGTGCCTCTGGTTCAACGAGGTGGTTCGGTTCACTTCTCGTGATCAGCTTAGTTTCCCATATGTATTATGGCGGTTGAAAGTGCTCAAGAAGATAAATATATTCCCTGTATGTACCCGGAAAGATCTTGTTAATAGTATGGGTCACATACGCAAGGCTAAGCCACTTAGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616 Dirigent

Protein Analysis

480

Amino Acids

54.56

Weight (kDa)

9.48

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 176 - 470 1.1e-118 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 468
AccII CGCG 1 cut(s) 435
AciI CCGC 4 cut(s) 47, 824, 1162, 1336
AclI AACGTT 1 cut(s) 379
AclWI GGATC 3 cut(s) 221, 582, 916
AcoI YGGCCR 2 cut(s) 58, 100
AcsI RAATTY 2 cut(s) 328, 423
AcuI CTGAAG 2 cut(s) 423, 1236
AfaI GTAC 5 cut(s) 155, 677, 814, 1050, 1378
AfiI CCNNNNNNNGG 4 cut(s) 185, 497, 996, 1032
AflIII ACRYGT 1 cut(s) 707
AgsI TTSAA 6 cut(s) 334, 984, 1041, 1195, 1279, 1342
AjuI GAANNNNNNNTTGG 6 cut(s) 215, 247, 290, 322, 532, 564
AluBI AGCT 3 cut(s) 145, 1073, 1312
AluI AGCT 3 cut(s) 145, 1073, 1312
Alw21I GWGCWC 4 cut(s) 528, 1020, 1075, 1350
Alw26I GTCTC 1 cut(s) 37
AlwI GGATC 3 cut(s) 221, 582, 916
AlwNI CAGNNNCTG 1 cut(s) 156
Ama87I CYCGRG 2 cut(s) 301, 354
AoxI GGCC 5 cut(s) 58, 100, 180, 534, 1122
ApoI RAATTY 2 cut(s) 328, 423
Asp700I GAANNNNTTC 1 cut(s) 119
AspLEI GCGC 3 cut(s) 90, 435, 870
AspS9I GGNCC 3 cut(s) 181, 535, 1003
AsuC2I CCSGG 2 cut(s) 179, 1381
AsuHPI GGTGA 1 cut(s) 304
AsuII TTCGAA 2 cut(s) 79, 769
AvaI CYCGRG 2 cut(s) 301, 354
AvaII GGWCC 1 cut(s) 1003
AxyI CCTNAGG 1 cut(s) 1009
BaeGI GKGCMC 1 cut(s) 163
BanII GRGCYC 1 cut(s) 1075
BauI CACGAG 1 cut(s) 1302
BbsI GAAGAC 2 cut(s) 672, 1188
Bbv12I GWGCWC 4 cut(s) 528, 1020, 1075, 1350
BccI CCATC 4 cut(s) 317, 836, 948, 1163
BcgI CGANNNNNNTGC 4 cut(s) 79, 113, 759, 793
BclI TGATCA 1 cut(s) 1306
BcnI CCSGG 2 cut(s) 179, 1381
BcoDI GTCTC 1 cut(s) 37
BfaI CTAG 1 cut(s) 138
BfmI CTRYAG 1 cut(s) 631
BglI GCCNNNNNGGC 1 cut(s) 1170
BglII AGATCT 2 cut(s) 886, 1387
BisI GCNGC 1 cut(s) 825
BlpI GCTNAGC 1 cut(s) 1422
BlsI GCNGC 1 cut(s) 826
Bme1390I CCNGG 2 cut(s) 179, 1381
Bme18I GGWCC 1 cut(s) 1003
BmeT110I CYCGRG 2 cut(s) 301, 354
BmgT120I GGNCC 3 cut(s) 181, 535, 1003
BmiI GGNNCC 3 cut(s) 537, 1004, 1005
BmrFI CCNGG 2 cut(s) 179, 1381
BmrI ACTGGG 1 cut(s) 166
BmsI GCATC 4 cut(s) 54, 424, 770, 1229
BmuI ACTGGG 1 cut(s) 166
BpiI GAAGAC 2 cut(s) 672, 1188
Bpu1102I GCTNAGC 1 cut(s) 1422
Bpu14I TTCGAA 2 cut(s) 79, 769
BpuEI CTTGAG 3 cut(s) 95, 431, 1334
BpuMI CCSGG 2 cut(s) 179, 1381
BsaBI GATNNNNATC 1 cut(s) 735
BsaJI CCNNGG 1 cut(s) 184
Bsc4I CCNNNNNNNGG 4 cut(s) 185, 497, 996, 1032
Bse1I ACTGG 2 cut(s) 161, 562
Bse21I CCTNAGG 1 cut(s) 1009
Bse8I GATNNNNATC 1 cut(s) 735
BseDI CCNNGG 1 cut(s) 184
BseGI GGATG 3 cut(s) 69, 328, 802
BseJI GATNNNNATC 1 cut(s) 735
BseLI CCNNNNNNNGG 4 cut(s) 185, 497, 996, 1032
BseMII CTCAG 4 cut(s) 42, 265, 533, 1074
BseNI ACTGG 2 cut(s) 161, 562
BseSI GKGCMC 1 cut(s) 163
BseX3I CGGCCG 1 cut(s) 58
Bsh1236I CGCG 1 cut(s) 435
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 5 cut(s) 60, 102, 182, 536, 1124
BsiEI CGRYCG 1 cut(s) 61
BsiHKAI GWGCWC 4 cut(s) 528, 1020, 1075, 1350
BsiHKCI CYCGRG 2 cut(s) 301, 354
BsiSI CCGG 4 cut(s) 61, 179, 260, 1381
BslFI GGGAC 2 cut(s) 973, 1016
BslI CCNNNNNNNGG 4 cut(s) 185, 497, 996, 1032
BsmAI GTCTC 1 cut(s) 37
BsmFI GGGAC 2 cut(s) 973, 1016
BsnI GGCC 5 cut(s) 60, 102, 182, 536, 1124
BsoBI CYCGRG 2 cut(s) 301, 354
Bsp119I TTCGAA 2 cut(s) 79, 769
Bsp1286I GDGCHC 5 cut(s) 163, 528, 1020, 1075, 1350
Bsp143I GATC 8 cut(s) 213, 343, 574, 730, 886, 921, 1306, 1387
Bsp1720I GCTNAGC 1 cut(s) 1422
BspACI CCGC 4 cut(s) 47, 824, 1162, 1336
BspANI GGCC 5 cut(s) 60, 102, 182, 536, 1124
BspCNI CTCAG 4 cut(s) 41, 264, 532, 1073
BspFNI CGCG 1 cut(s) 435
BspLI GGNNCC 3 cut(s) 537, 1004, 1005
BspPI GGATC 3 cut(s) 221, 582, 916
BspT104I TTCGAA 2 cut(s) 79, 769
BsrI ACTGG 2 cut(s) 161, 562
BssECI CCNNGG 1 cut(s) 184
BssMI GATC 8 cut(s) 213, 343, 574, 730, 886, 921, 1306, 1387
BssNAI GTATAC 1 cut(s) 469
BssSI CACGAG 1 cut(s) 1302
BssT1I CCWWGG 1 cut(s) 184
Bst1107I GTATAC 1 cut(s) 469
Bst2BI CACGAG 1 cut(s) 1302
Bst4CI ACNGT 5 cut(s) 292, 512, 610, 623, 635
BstBI TTCGAA 2 cut(s) 79, 769
BstC8I GCNNGC 2 cut(s) 1075, 1175
BstDEI CTNAG 9 cut(s) 28, 251, 519, 809, 1009, 1060, 1313, 1422, 1430
BstF5I GGATG 3 cut(s) 69, 328, 802
BstFNI CGCG 1 cut(s) 435
BstHHI GCGC 3 cut(s) 90, 435, 870
BstKTI GATC 8 cut(s) 216, 346, 577, 733, 889, 924, 1309, 1390
BstMAI GTCTC 1 cut(s) 37
BstMBI GATC 8 cut(s) 213, 343, 574, 730, 886, 921, 1306, 1387
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 3 cut(s) 15, 265, 1170
BstNSI RCATGY 1 cut(s) 711
BstSCI CCNGG 2 cut(s) 177, 1379
BstSFI CTRYAG 1 cut(s) 631
BstSLI GKGCMC 1 cut(s) 163
BstUI CGCG 1 cut(s) 435
BstV2I GAAGAC 2 cut(s) 672, 1188
BstX2I RGATCY 3 cut(s) 886, 921, 1387
BstYI RGATCY 3 cut(s) 886, 921, 1387
BstZ17I GTATAC 1 cut(s) 469
BstZI CGGCCG 1 cut(s) 58
Bsu36I CCTNAGG 1 cut(s) 1009
BsuRI GGCC 5 cut(s) 60, 102, 182, 536, 1124
BtsCI GGATG 3 cut(s) 69, 328, 802
BtsIMutI CAGTG 2 cut(s) 259, 640
Cac8I GCNNGC 2 cut(s) 1075, 1175
CaiI CAGNNNCTG 1 cut(s) 156
CfoI GCGC 3 cut(s) 90, 435, 870
Cfr13I GGNCC 3 cut(s) 181, 535, 1003
Csp6I GTAC 5 cut(s) 154, 676, 813, 1049, 1377
CviAII CATG 5 cut(s) 679, 708, 956, 1067, 1166
CviQI GTAC 5 cut(s) 154, 676, 813, 1049, 1377
DdeI CTNAG 9 cut(s) 28, 251, 519, 809, 1009, 1060, 1313, 1422, 1430
DpnI GATC 8 cut(s) 215, 345, 576, 732, 888, 923, 1308, 1389
DpnII GATC 8 cut(s) 213, 343, 574, 730, 886, 921, 1306, 1387
DraI TTTAAA 1 cut(s) 652
EaeI YGGCCR 2 cut(s) 58, 100
EagI CGGCCG 1 cut(s) 58
Ecl136II GAGCTC 1 cut(s) 1073
EclXI CGGCCG 1 cut(s) 58
Eco130I CCWWGG 1 cut(s) 184
Eco24I GRGCYC 1 cut(s) 1075
Eco47I GGWCC 1 cut(s) 1003
Eco52I CGGCCG 1 cut(s) 58
Eco53kI GAGCTC 1 cut(s) 1073
Eco57I CTGAAG 2 cut(s) 423, 1236
Eco81I CCTNAGG 1 cut(s) 1009
Eco88I CYCGRG 2 cut(s) 301, 354
EcoICRI GAGCTC 1 cut(s) 1073
EcoO109I RGGNCCY 2 cut(s) 535, 1003
EcoT14I CCWWGG 1 cut(s) 184
EcoT38I GRGCYC 1 cut(s) 1075
ErhI CCWWGG 1 cut(s) 184
FaeI CATG 5 cut(s) 682, 711, 959, 1070, 1169
FalI AAGNNNNNCTT 2 cut(s) 1014, 1046
FaqI GGGAC 2 cut(s) 973, 1016
FatI CATG 5 cut(s) 678, 707, 955, 1066, 1165
FauNDI CATATG 1 cut(s) 1324
FbaI TGATCA 1 cut(s) 1306
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 1 cut(s) 825
FokI GGATG 3 cut(s) 76, 335, 809
FriOI GRGCYC 1 cut(s) 1075
Fsp4HI GCNGC 1 cut(s) 825
FspBI CTAG 1 cut(s) 138
GlaI GCGC 3 cut(s) 89, 434, 869
GluI GCNGC 1 cut(s) 825
HaeIII GGCC 5 cut(s) 60, 102, 182, 536, 1124
HapII CCGG 4 cut(s) 61, 179, 260, 1381
HhaI GCGC 3 cut(s) 90, 435, 870
Hin1II CATG 5 cut(s) 682, 711, 959, 1070, 1169
Hin6I GCGC 3 cut(s) 88, 433, 868
HinP1I GCGC 3 cut(s) 88, 433, 868
HinfI GANTC 8 cut(s) 69, 108, 132, 334, 352, 440, 980, 1153
HpaII CCGG 4 cut(s) 61, 179, 260, 1381
HphI GGTGA 1 cut(s) 304
Hpy166II GTNNAC 4 cut(s) 469, 705, 815, 1296
Hpy188III TCNNGA 9 cut(s) 112, 401, 419, 498, 521, 572, 1231, 1304, 1351
Hpy8I GTNNAC 4 cut(s) 469, 705, 815, 1296
Hpy99I CGWCG 2 cut(s) 41, 44
HpyAV CCTTC 2 cut(s) 1093, 1198
HpyCH4III ACNGT 5 cut(s) 292, 512, 610, 623, 635
HpyCH4IV ACGT 1 cut(s) 379
HpyCH4V TGCA 4 cut(s) 690, 713, 959, 1147
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 265, 1170
HpyF3I CTNAG 9 cut(s) 28, 251, 519, 809, 1009, 1060, 1313, 1422, 1430
HpySE526I ACGT 1 cut(s) 379
Hsp92II CATG 5 cut(s) 682, 711, 959, 1070, 1169
HspAI GCGC 3 cut(s) 88, 433, 868
KflI GGGWCCC 1 cut(s) 1003
Ksp22I TGATCA 1 cut(s) 1306
Kzo9I GATC 8 cut(s) 213, 343, 574, 730, 886, 921, 1306, 1387
LmnI GCTCC 3 cut(s) 523, 1070, 1237
LweI GCATC 4 cut(s) 54, 424, 770, 1229
MaeI CTAG 1 cut(s) 138
MaeII ACGT 1 cut(s) 379
MaeIII GTNAC 2 cut(s) 610, 1406
MalI GATC 8 cut(s) 215, 345, 576, 732, 888, 923, 1308, 1389
MboI GATC 8 cut(s) 213, 343, 574, 730, 886, 921, 1306, 1387
MfeI CAATTG 1 cut(s) 1148
MflI RGATCY 3 cut(s) 886, 921, 1387
MhlI GDGCHC 5 cut(s) 163, 528, 1020, 1075, 1350
MluCI AATT 7 cut(s) 328, 423, 547, 801, 837, 992, 1148
MlyI GAGTC 5 cut(s) 78, 102, 141, 346, 1147
MmeI TCCRAC 3 cut(s) 60, 225, 343
MroXI GAANNNNTTC 1 cut(s) 119
MseI TTAA 7 cut(s) 456, 546, 594, 651, 882, 911, 1395
MspI CCGG 4 cut(s) 61, 179, 260, 1381
MspR9I CCNGG 2 cut(s) 179, 1381
MunI CAATTG 1 cut(s) 1148
MvnI CGCG 1 cut(s) 435
MwoI GCNNNNNNNGC 3 cut(s) 15, 265, 1170
NciI CCSGG 2 cut(s) 179, 1381
NdeI CATATG 1 cut(s) 1324
NdeII GATC 8 cut(s) 213, 343, 574, 730, 886, 921, 1306, 1387
NlaIII CATG 5 cut(s) 682, 711, 959, 1070, 1169
NlaIV GGNNCC 3 cut(s) 537, 1004, 1005
NmeAIII GCCGAG 4 cut(s) 36, 78, 252, 294
NmuCI GTSAC 1 cut(s) 1406
NspI RCATGY 1 cut(s) 711
NspV TTCGAA 2 cut(s) 79, 769
PaeR7I CTCGAG 1 cut(s) 301
PciI ACATGT 1 cut(s) 707
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 3 cut(s) 334, 440, 980
PkrI GCNGC 1 cut(s) 826
PleI GAGTC 5 cut(s) 77, 102, 140, 346, 1147
PpsI GAGTC 5 cut(s) 77, 102, 140, 346, 1147
PpuMI RGGWCCY 1 cut(s) 1003
PscI ACATGT 1 cut(s) 707
Psp124BI GAGCTC 1 cut(s) 1075
Psp1406I AACGTT 1 cut(s) 379
Psp5II RGGWCCY 1 cut(s) 1003
PspN4I GGNNCC 3 cut(s) 537, 1004, 1005
PspPI GGNCC 3 cut(s) 181, 535, 1003
PspPPI RGGWCCY 1 cut(s) 1003
PspXI VCTCGAGB 1 cut(s) 301
PstNI CAGNNNCTG 1 cut(s) 156
PsuI RGATCY 3 cut(s) 886, 921, 1387
RsaI GTAC 5 cut(s) 155, 677, 814, 1050, 1378
RsaNI GTAC 5 cut(s) 154, 676, 813, 1049, 1377
SacI GAGCTC 1 cut(s) 1075
SaqAI TTAA 7 cut(s) 456, 546, 594, 651, 882, 911, 1395
SatI GCNGC 1 cut(s) 825
Sau3AI GATC 8 cut(s) 213, 343, 574, 730, 886, 921, 1306, 1387
Sau96I GGNCC 3 cut(s) 181, 535, 1003
SchI GAGTC 5 cut(s) 78, 102, 141, 346, 1147
ScrFI CCNGG 2 cut(s) 179, 1381
SduI GDGCHC 5 cut(s) 163, 528, 1020, 1075, 1350
SfaNI GCATC 4 cut(s) 54, 424, 770, 1229
SfcI CTRYAG 1 cut(s) 631
Sfr274I CTCGAG 1 cut(s) 301
SfuI TTCGAA 2 cut(s) 79, 769
SinI GGWCC 1 cut(s) 1003
SlaI CTCGAG 1 cut(s) 301
SmlI CTYRAG 4 cut(s) 110, 301, 410, 1349
SmoI CTYRAG 4 cut(s) 110, 301, 410, 1349
Sse9I AATT 7 cut(s) 328, 423, 547, 801, 837, 992, 1148
SsiI CCGC 4 cut(s) 47, 824, 1162, 1336
SspMI CTAG 1 cut(s) 138
SstI GAGCTC 1 cut(s) 1075
StyD4I CCNGG 2 cut(s) 177, 1379
StyI CCWWGG 1 cut(s) 184
TaaI ACNGT 5 cut(s) 292, 512, 610, 623, 635
TaiI ACGT 1 cut(s) 382
TaqI TCGA 6 cut(s) 79, 209, 302, 342, 769, 1020
TasI AATT 7 cut(s) 328, 423, 547, 801, 837, 992, 1148
TatI WGTACW 2 cut(s) 812, 1048
TauI GCSGC 1 cut(s) 827
TfiI GAWTC 3 cut(s) 334, 440, 980
Tru1I TTAA 7 cut(s) 456, 546, 594, 651, 882, 911, 1395
Tru9I TTAA 7 cut(s) 456, 546, 594, 651, 882, 911, 1395
TscAI CASTG 2 cut(s) 259, 640
TseFI GTSAC 1 cut(s) 1406
Tsp45I GTSAC 1 cut(s) 1406
TspDTI ATGAA 6 cut(s) 540, 618, 632, 678, 813, 1107
TspGWI ACGGA 2 cut(s) 480, 1212
TspRI CASTG 2 cut(s) 259, 640
VpaK11BI GGWCC 1 cut(s) 1003
XapI RAATTY 2 cut(s) 328, 423
XceI RCATGY 1 cut(s) 711
XcmI CCANNNNNNNNNTGG 2 cut(s) 911, 1329
XhoI CTCGAG 1 cut(s) 301
XmiI GTMKAC 1 cut(s) 468
XmnI GAANNNNTTC 1 cut(s) 119
XspI CTAG 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.