Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0002998

Physical Location & Seq

Genomic Coordinates Forward (+)

23302257 .. 23305445

Gene Structure 3189 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g25290.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1392 bp

ATGTTTAACAACAACAGCATATCAATCTCAGTCTCCGACGACGAAGCGAACGAGCTCGGCCGGATGAGAGTCCGAGTTCGAAGAAAGCGCAAGCAACTCGGCCAACGACTCAAGCACCGAATCGCTAGAAAGCTCGTCAGGTACTGGGCGCTTCTCATTGTTCTTCCGGCCCTCGGGTTGTTTCTGTTCGAGGCTTCCAGGATCGGCAAGAAGCCGAGTTTGGTTGAGTCGGAACGCAATACGGTGAAGAACCCGAGTTTGGAGAAGAACTCGGATGCGAATTTGAATCGGCTTGATCGTACGACTCGGGTTGTTGGAGGCGTTAGACAACGTTGTTTGGACATTCTATCTCCTGAAGAACTTGAGCAACTCGAAATTCCTGTGCGTGAAGAGTCCGATAGTCCTGTTAAGAACGTATTATACAGATCAGAGAATGACACTTCGTTTTTAGGAGGGAACAACAGTCTTTCTCAAGAGCAACCAGAGGCCACAAGATTTAATTCATTTACTGGAACTCAAACACTAGATCAGAGGGAGAAATCGTTTAAGGTAAATGAAACTGTTACTATGAACTGTGGTTTCTACAGTGAAAATGGAGGGTTTAAAATCTCAAATGCAGACATGGAGTACATGAATAGTTGCAAGGTTGTAGTGTCCACATGTGCATTTGGTGGTGGAGATGATCTTTATCAGCCTATTGGAATGTCAGAGGCTTCACTTCGAAAGGTTTGCTATGTTGCTTTTTGGGATGATATTACTCTAAGTACACAGGAAGCGGCTGAACGTAGAATTGATGGGGATGGATTTGTTGGGAAATGGCGTGTTGTGGTTGTTAAAGATCTTCCCTTTACGGACCAAAGGTTAAATGGAAAGATCCCAAAGATGTTGCCCCATCGTCTTTTTCCTCATGCAAAGTATTCCATTTGGGTAGACTCAAAGTCCCAATTTAGGAGGGACCCTTTAGGTGTGCTCGAAGCCCTGCTTTGGCGTTCAAATTCTGTACTTGCGATCTCAGAACATGGAGCACGGAGTAGCGTTTATGATGAGGCGAAGGCTGTTGTAAAAAAAAACAAGGCCAAACCAGAAGAAGTTGAGGTGCAGTTGACTCAATACCGACATGATGGCTTGCCAGAAGACAAGAGGTTCAACGGAAAGAAGGCTTTATCTGAAGCATCTGTTATTGTGAGGGAGCATACACTCCTGACTAATATGTTTACGTGCCTCTGGTTCAACGAGGTGGTTCGATTCACTTCTCGTGACCAGCTTAGTTTCCCATATGTATTATGGCGGCTGAAAGTGCTCAAGAACATCAACGTGTTCCCTGTATGTACCCGGAAAGATCTTGTTAATAGCATGGGTCACATACGCAAGGCCAAGCCACTGAGAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF616 Dirigent

Protein Analysis

464

Amino Acids

52.91

Weight (kDa)

9.51

Isoelectric Point (pI)

42.0

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002998)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G42660
fragaria_vesca	FvH4_7g01740 FvH4_7g01740 FvH4_7g01740 FvH4_7g01740
malus_domestica	MD02G1301200.v1.1 MD07G1022800.v1.1
prunus_persica	Prupe.2G023300_v2.0.a1 Prupe.2G023300_v2.0.a1 Prupe.2G023300_v2.0.a1 Prupe.2G023300_v2.0.a1 Prupe.2G023300_v2.0.a1
pyrus_communis	pycom02g25290 pycom07g01730
rosa_chinensis	RchiOBHm_Chr1g0318301 RchiOBHm_Chr4g0418501
rosa_laevigata	RLG00000007859 RLG00000030546
rosa_multiflora	Rmu_sc0000363.1_g000055 Rmu_sc0001879.1_g000028 Rmu_ssc0000308.1_g000013
rosa_roxburghii	Rroxscaffold_4G00329380 Rroxscaffold_4G00329420 Rroxscaffold_4G00329640 Rroxscaffold_5G00361730
rosa_rugosa	Rorug01G0020100 Rorug01G0020100 Rorug04G0153900
rosa_samantha	Rh1AG032200 Rh1BG027900 Rh1CG030700 Rh1DG039000 Rh4AG214900 Rh4BG215500 Rh4CG226600 Rh4DG213500
rosa_wichuraiana	Rw0G010070 Rw1G002580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	930
AciI	CCGC	2 cut(s)	776, 1288
AclI	AACGTT	1 cut(s)	331
AclWI	GGATC	2 cut(s)	209, 868
AcoI	YGGCCR	2 cut(s)	58, 100
AcsI	RAATTY	3 cut(s)	280, 375, 994
AcuI	CTGAAG	2 cut(s)	375, 1188
AfaI	GTAC	6 cut(s)	143, 301, 629, 766, 1002, 1330
AfiI	CCNNNNNNNGG	4 cut(s)	173, 259, 948, 984
AflIII	ACRYGT	2 cut(s)	659, 1314
AgsI	TTSAA	4 cut(s)	286, 993, 1147, 1231
AhdI	GACNNNNNGTC	1 cut(s)	937
AjnI	CCWGG	1 cut(s)	197
AjuI	GAANNNNNNNTTGG	4 cut(s)	203, 235, 242, 274
AluBI	AGCT	3 cut(s)	55, 133, 1264
AluI	AGCT	3 cut(s)	55, 133, 1264
Alw21I	GWGCWC	4 cut(s)	57, 972, 1027, 1302
Alw26I	GTCTC	1 cut(s)	37
AlwI	GGATC	2 cut(s)	209, 868
AlwNI	CAGNNNCTG	1 cut(s)	144
Ama87I	CYCGRG	3 cut(s)	173, 253, 306
AoxI	GGCC	6 cut(s)	58, 100, 168, 486, 1074, 1371
ApoI	RAATTY	3 cut(s)	280, 375, 994
AspLEI	GCGC	2 cut(s)	90, 151
AspS9I	GGNCC	3 cut(s)	169, 853, 955
AsuC2I	CCSGG	1 cut(s)	1333
AsuHPI	GGTGA	1 cut(s)	256
AsuII	TTCGAA	2 cut(s)	79, 721
AvaI	CYCGRG	3 cut(s)	173, 253, 306
AvaII	GGWCC	2 cut(s)	853, 955
BanII	GRGCYC	1 cut(s)	57
BauI	CACGAG	1 cut(s)	1254
BbsI	GAAGAC	1 cut(s)	1140
Bbv12I	GWGCWC	4 cut(s)	57, 972, 1027, 1302
BccI	CCATC	4 cut(s)	788, 794, 900, 1115
BcgI	CGANNNNNNTGC	4 cut(s)	79, 113, 711, 745
BciT130I	CCWGG	1 cut(s)	199
BcnI	CCSGG	1 cut(s)	1333
BcoDI	GTCTC	1 cut(s)	37
BfaI	CTAG	2 cut(s)	126, 524
BfmI	CTRYAG	1 cut(s)	583
BfoI	RGCGCY	1 cut(s)	152
BglII	AGATCT	2 cut(s)	838, 1339
BisI	GCNGC	2 cut(s)	777, 1289
BlsI	GCNGC	2 cut(s)	778, 1290
Bme1390I	CCNGG	2 cut(s)	199, 1333
Bme18I	GGWCC	2 cut(s)	853, 955
BmeRI	GACNNNNNGTC	1 cut(s)	937
BmeT110I	CYCGRG	3 cut(s)	173, 253, 306
BmgT120I	GGNCC	3 cut(s)	169, 853, 955
BmiI	GGNNCC	2 cut(s)	956, 957
BmrFI	CCNGG	2 cut(s)	199, 1333
BmrI	ACTGGG	1 cut(s)	154
BmsI	GCATC	2 cut(s)	265, 1181
BmuI	ACTGGG	1 cut(s)	154
BpiI	GAAGAC	1 cut(s)	1140
BplI	GAGNNNNNCTC	2 cut(s)	254, 286
Bpu14I	TTCGAA	2 cut(s)	79, 721
BpuEI	CTTGAG	4 cut(s)	95, 383, 456, 1286
BpuMI	CCSGG	1 cut(s)	1333
BsaAI	YACGTR	1 cut(s)	1218
BsaBI	GATNNNNATC	1 cut(s)	687
BsaJI	CCNNGG	1 cut(s)	172
BsaXI	ACNNNNNCTCC	2 cut(s)	527, 557
Bsc4I	CCNNNNNNNGG	4 cut(s)	173, 259, 948, 984
Bse1I	ACTGG	2 cut(s)	149, 514
Bse8I	GATNNNNATC	1 cut(s)	687
BseBI	CCWGG	1 cut(s)	199
BseDI	CCNNGG	1 cut(s)	172
BseGI	GGATG	4 cut(s)	69, 280, 754, 805
BseJI	GATNNNNATC	1 cut(s)	687
BseLI	CCNNNNNNNGG	4 cut(s)	173, 259, 948, 984
BseMII	CTCAG	3 cut(s)	42, 1026, 1373
BseNI	ACTGG	2 cut(s)	149, 514
BseX3I	CGGCCG	1 cut(s)	58
BsgI	GTGCAG	1 cut(s)	1118
Bsh1285I	CGRYCG	1 cut(s)	61
BshFI	GGCC	6 cut(s)	60, 102, 170, 488, 1076, 1373
BsiEI	CGRYCG	1 cut(s)	61
BsiHKAI	GWGCWC	4 cut(s)	57, 972, 1027, 1302
BsiHKCI	CYCGRG	3 cut(s)	173, 253, 306
BsiSI	CCGG	3 cut(s)	61, 167, 1333
BsiWI	CGTACG	1 cut(s)	299
BslFI	GGGAC	2 cut(s)	925, 968
BslI	CCNNNNNNNGG	4 cut(s)	173, 259, 948, 984
BsmAI	GTCTC	1 cut(s)	37
BsmFI	GGGAC	2 cut(s)	925, 968
BsnI	GGCC	6 cut(s)	60, 102, 170, 488, 1076, 1373
BsoBI	CYCGRG	3 cut(s)	173, 253, 306
Bsp119I	TTCGAA	2 cut(s)	79, 721
Bsp1286I	GDGCHC	4 cut(s)	57, 972, 1027, 1302
Bsp143I	GATC	9 cut(s)	201, 295, 425, 526, 682, 838, 873, 1008, 1339
BspACI	CCGC	2 cut(s)	776, 1288
BspANI	GGCC	6 cut(s)	60, 102, 170, 488, 1076, 1373
BspCNI	CTCAG	3 cut(s)	41, 1025, 1374
BspLI	GGNNCC	2 cut(s)	956, 957
BspPI	GGATC	2 cut(s)	209, 868
BspT104I	TTCGAA	2 cut(s)	79, 721
BsrI	ACTGG	2 cut(s)	149, 514
BssECI	CCNNGG	1 cut(s)	172
BssMI	GATC	9 cut(s)	201, 295, 425, 526, 682, 838, 873, 1008, 1339
BssSI	CACGAG	1 cut(s)	1254
Bst2BI	CACGAG	1 cut(s)	1254
Bst2UI	CCWGG	1 cut(s)	199
Bst4CI	ACNGT	5 cut(s)	244, 464, 562, 575, 587
Bst6I	CTCTTC	1 cut(s)	384
BstBAI	YACGTR	1 cut(s)	1218
BstBI	TTCGAA	2 cut(s)	79, 721
BstC8I	GCNNGC	2 cut(s)	92, 1127
BstDEI	CTNAG	5 cut(s)	28, 761, 1012, 1265, 1382
BstF5I	GGATG	4 cut(s)	69, 280, 754, 805
BstH2I	RGCGCY	1 cut(s)	152
BstHHI	GCGC	2 cut(s)	90, 151
BstKTI	GATC	9 cut(s)	204, 298, 428, 529, 685, 841, 876, 1011, 1342
BstMAI	GTCTC	1 cut(s)	37
BstMBI	GATC	9 cut(s)	201, 295, 425, 526, 682, 838, 873, 1008, 1339
BstMCI	CGRYCG	1 cut(s)	61
BstMWI	GCNNNNNNNGC	1 cut(s)	1297
BstNI	CCWGG	1 cut(s)	199
BstNSI	RCATGY	1 cut(s)	663
BstSCI	CCNGG	2 cut(s)	197, 1331
BstSFI	CTRYAG	1 cut(s)	583
BstV2I	GAAGAC	1 cut(s)	1140
BstX2I	RGATCY	3 cut(s)	838, 873, 1339
BstYI	RGATCY	3 cut(s)	838, 873, 1339
BstZI	CGGCCG	1 cut(s)	58
BsuRI	GGCC	6 cut(s)	60, 102, 170, 488, 1076, 1373
BtsCI	GGATG	4 cut(s)	69, 280, 754, 805
BtsIMutI	CAGTG	2 cut(s)	592, 1379
Cac8I	GCNNGC	2 cut(s)	92, 1127
CaiI	CAGNNNCTG	1 cut(s)	144
CfoI	GCGC	2 cut(s)	90, 151
Cfr13I	GGNCC	3 cut(s)	169, 853, 955
Csp6I	GTAC	6 cut(s)	142, 300, 628, 765, 1001, 1329
CviAII	CATG	7 cut(s)	622, 631, 660, 908, 1019, 1118, 1354
CviQI	GTAC	6 cut(s)	142, 300, 628, 765, 1001, 1329
DdeI	CTNAG	5 cut(s)	28, 761, 1012, 1265, 1382
DpnI	GATC	9 cut(s)	203, 297, 427, 528, 684, 840, 875, 1010, 1341
DpnII	GATC	9 cut(s)	201, 295, 425, 526, 682, 838, 873, 1008, 1339
DraI	TTTAAA	1 cut(s)	604
DriI	GACNNNNNGTC	1 cut(s)	937
EaeI	YGGCCR	2 cut(s)	58, 100
EagI	CGGCCG	1 cut(s)	58
Eam1104I	CTCTTC	1 cut(s)	384
Eam1105I	GACNNNNNGTC	1 cut(s)	937
EarI	CTCTTC	1 cut(s)	384
Ecl136II	GAGCTC	1 cut(s)	55
EclXI	CGGCCG	1 cut(s)	58
Eco24I	GRGCYC	1 cut(s)	57
Eco47I	GGWCC	2 cut(s)	853, 955
Eco52I	CGGCCG	1 cut(s)	58
Eco53kI	GAGCTC	1 cut(s)	55
Eco57I	CTGAAG	2 cut(s)	375, 1188
Eco88I	CYCGRG	3 cut(s)	173, 253, 306
EcoICRI	GAGCTC	1 cut(s)	55
EcoO109I	RGGNCCY	1 cut(s)	955
EcoRII	CCWGG	1 cut(s)	197
EcoT38I	GRGCYC	1 cut(s)	57
FaeI	CATG	7 cut(s)	625, 634, 663, 911, 1022, 1121, 1357
FalI	AAGNNNNNCTT	2 cut(s)	966, 998
FaqI	GGGAC	2 cut(s)	925, 968
FatI	CATG	7 cut(s)	621, 630, 659, 907, 1018, 1117, 1353
FauNDI	CATATG	1 cut(s)	1276
FblI	GTMKAC	1 cut(s)	930
Fnu4HI	GCNGC	2 cut(s)	777, 1289
FokI	GGATG	4 cut(s)	76, 287, 761, 812
FriOI	GRGCYC	1 cut(s)	57
Fsp4HI	GCNGC	2 cut(s)	777, 1289
FspBI	CTAG	2 cut(s)	126, 524
GlaI	GCGC	2 cut(s)	89, 150
GluI	GCNGC	2 cut(s)	777, 1289
HaeII	RGCGCY	1 cut(s)	152
HaeIII	GGCC	6 cut(s)	60, 102, 170, 488, 1076, 1373
HapII	CCGG	3 cut(s)	61, 167, 1333
HhaI	GCGC	2 cut(s)	90, 151
Hin1II	CATG	7 cut(s)	625, 634, 663, 911, 1022, 1121, 1357
Hin6I	GCGC	2 cut(s)	88, 149
HinP1I	GCGC	2 cut(s)	88, 149
HincII	GTYRAC	1 cut(s)	1104
HindII	GTYRAC	1 cut(s)	1104
HpaII	CCGG	3 cut(s)	61, 167, 1333
HphI	GGTGA	1 cut(s)	256
Hpy166II	GTNNAC	5 cut(s)	657, 767, 931, 1104, 1215
Hpy188III	TCNNGA	5 cut(s)	353, 473, 1201, 1256, 1303
Hpy8I	GTNNAC	5 cut(s)	657, 767, 931, 1104, 1215
Hpy99I	CGWCG	2 cut(s)	41, 44
HpyAV	CCTTC	2 cut(s)	1045, 1150
HpyCH4III	ACNGT	5 cut(s)	244, 464, 562, 575, 587
HpyCH4IV	ACGT	5 cut(s)	331, 414, 784, 1217, 1314
HpyCH4V	TGCA	5 cut(s)	617, 642, 665, 911, 1099
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1297
HpyF3I	CTNAG	5 cut(s)	28, 761, 1012, 1265, 1382
HpySE526I	ACGT	5 cut(s)	331, 414, 784, 1217, 1314
Hsp92II	CATG	7 cut(s)	625, 634, 663, 911, 1022, 1121, 1357
HspAI	GCGC	2 cut(s)	88, 149
KflI	GGGWCCC	1 cut(s)	955
Kzo9I	GATC	9 cut(s)	201, 295, 425, 526, 682, 838, 873, 1008, 1339
LmnI	GCTCC	2 cut(s)	1022, 1189
LweI	GCATC	2 cut(s)	265, 1181
MaeI	CTAG	2 cut(s)	126, 524
MaeII	ACGT	5 cut(s)	331, 414, 784, 1217, 1314
MaeIII	GTNAC	3 cut(s)	562, 1256, 1358
MalI	GATC	9 cut(s)	203, 297, 427, 528, 684, 840, 875, 1010, 1341
MboI	GATC	9 cut(s)	201, 295, 425, 526, 682, 838, 873, 1008, 1339
MboII	GAAGA	9 cut(s)	93, 155, 259, 277, 368, 401, 833, 1097, 1145
MflI	RGATCY	3 cut(s)	838, 873, 1339
MhlI	GDGCHC	4 cut(s)	57, 972, 1027, 1302
MluCI	AATT	6 cut(s)	280, 375, 499, 789, 944, 994
MlyI	GAGTC	7 cut(s)	78, 102, 236, 298, 401, 926, 1099
MmeI	TCCRAC	3 cut(s)	60, 210, 295
MseI	TTAA	8 cut(s)	6, 408, 498, 546, 603, 834, 863, 1347
MslI	CAYNNNNRTG	1 cut(s)	1313
MspI	CCGG	3 cut(s)	61, 167, 1333
MspR9I	CCNGG	2 cut(s)	199, 1333
MvaI	CCWGG	1 cut(s)	199
MwoI	GCNNNNNNNGC	1 cut(s)	1297
NciI	CCSGG	1 cut(s)	1333
NdeI	CATATG	1 cut(s)	1276
NdeII	GATC	9 cut(s)	201, 295, 425, 526, 682, 838, 873, 1008, 1339
NlaIII	CATG	7 cut(s)	625, 634, 663, 911, 1022, 1121, 1357
NlaIV	GGNNCC	2 cut(s)	956, 957
NmeAIII	GCCGAG	3 cut(s)	36, 78, 240
NmuCI	GTSAC	2 cut(s)	1256, 1358
NspI	RCATGY	1 cut(s)	663
NspV	TTCGAA	2 cut(s)	79, 721
PciI	ACATGT	1 cut(s)	659
PcsI	WCGNNNNNNNCGW	2 cut(s)	48, 295
PfeI	GAWTC	3 cut(s)	120, 286, 1245
Pfl23II	CGTACG	1 cut(s)	299
PfoI	TCCNGGA	1 cut(s)	197
PkrI	GCNGC	2 cut(s)	778, 1290
PleI	GAGTC	7 cut(s)	77, 102, 235, 298, 400, 926, 1099
PpsI	GAGTC	7 cut(s)	77, 102, 235, 298, 400, 926, 1099
Ppu21I	YACGTR	1 cut(s)	1218
PpuMI	RGGWCCY	1 cut(s)	955
PscI	ACATGT	1 cut(s)	659
Psp124BI	GAGCTC	1 cut(s)	57
Psp1406I	AACGTT	1 cut(s)	331
Psp5II	RGGWCCY	1 cut(s)	955
Psp6I	CCWGG	1 cut(s)	197
PspGI	CCWGG	1 cut(s)	197
PspLI	CGTACG	1 cut(s)	299
PspN4I	GGNNCC	2 cut(s)	956, 957
PspPI	GGNCC	3 cut(s)	169, 853, 955
PspPPI	RGGWCCY	1 cut(s)	955
PsrI	GAACNNNNNNTAC	2 cut(s)	404, 436
PstNI	CAGNNNCTG	1 cut(s)	144
PsuI	RGATCY	3 cut(s)	838, 873, 1339
RsaI	GTAC	6 cut(s)	143, 301, 629, 766, 1002, 1330
RsaNI	GTAC	6 cut(s)	142, 300, 628, 765, 1001, 1329
RseI	CAYNNNNRTG	1 cut(s)	1313
SacI	GAGCTC	1 cut(s)	57
SaqAI	TTAA	8 cut(s)	6, 408, 498, 546, 603, 834, 863, 1347
SatI	GCNGC	2 cut(s)	777, 1289
Sau3AI	GATC	9 cut(s)	201, 295, 425, 526, 682, 838, 873, 1008, 1339
Sau96I	GGNCC	3 cut(s)	169, 853, 955
SchI	GAGTC	7 cut(s)	78, 102, 236, 298, 401, 926, 1099
ScrFI	CCNGG	2 cut(s)	199, 1333
SduI	GDGCHC	4 cut(s)	57, 972, 1027, 1302
SfaNI	GCATC	2 cut(s)	265, 1181
SfcI	CTRYAG	1 cut(s)	583
SfuI	TTCGAA	2 cut(s)	79, 721
SinI	GGWCC	2 cut(s)	853, 955
SmiMI	CAYNNNNRTG	1 cut(s)	1313
SmlI	CTYRAG	4 cut(s)	110, 362, 471, 1301
SmoI	CTYRAG	4 cut(s)	110, 362, 471, 1301
Sse9I	AATT	6 cut(s)	280, 375, 499, 789, 944, 994
SsiI	CCGC	2 cut(s)	776, 1288
SspMI	CTAG	2 cut(s)	126, 524
SstI	GAGCTC	1 cut(s)	57
StyD4I	CCNGG	2 cut(s)	197, 1331
TaaI	ACNGT	5 cut(s)	244, 464, 562, 575, 587
TaiI	ACGT	5 cut(s)	334, 417, 787, 1220, 1317
TaqI	TCGA	6 cut(s)	79, 189, 372, 721, 972, 1243
TasI	AATT	6 cut(s)	280, 375, 499, 789, 944, 994
TatI	WGTACW	3 cut(s)	627, 764, 1000
TauI	GCSGC	2 cut(s)	779, 1291
TfiI	GAWTC	3 cut(s)	120, 286, 1245
Tru1I	TTAA	8 cut(s)	6, 408, 498, 546, 603, 834, 863, 1347
Tru9I	TTAA	8 cut(s)	6, 408, 498, 546, 603, 834, 863, 1347
TscAI	CASTG	2 cut(s)	592, 1386
TseFI	GTSAC	2 cut(s)	1256, 1358
Tsp45I	GTSAC	2 cut(s)	1256, 1358
TspDTI	ATGAA	4 cut(s)	492, 570, 584, 647
TspGWI	ACGGA	3 cut(s)	866, 1042, 1164
TspRI	CASTG	2 cut(s)	592, 1386
VpaK11BI	GGWCC	2 cut(s)	853, 955
XapI	RAATTY	3 cut(s)	280, 375, 994
XceI	RCATGY	1 cut(s)	663
XcmI	CCANNNNNNNNNTGG	2 cut(s)	863, 1281
XmiI	GTMKAC	1 cut(s)	930
XspI	CTAG	2 cut(s)	126, 524

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.