Rh4BG215500

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002998
Physical Location & Seq
Forward (+)
37485007 .. 37487948
2942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG215500.1

Sequence Viewer

Length: 1251 bp
ATGATGAACAACAAGAGCATATCAATGGCAGTGTCGGATGACGAATCTGACGAGCTCGGCCGGATGCGCGTTCGAGTTCGCCGGAAGCGCAAGAAACTCCACCACCGATTCAAGAACGGAGTGGTGAGGAATCTGGTCAGCTACTGGCCCCTGTTCTGGGCCCTTGTAATTTTTCTAGCGGCCATTTGTTTGCTTCTGTATGAGGCATGGACTATCGGCTGGAAATCCCCACAACTCAATAACTCAGAACTCACTGCGGTGAACAATGATTTGAATCGGCTTGATCCTATCACTCAGGTTGTTCGTGGCGTTACGCAACGTTGCTTGCCAATCTTACCTCCTGAACAACTTGAGCATCTGGAAATTCCTGTGCCCCAAGATTCCACTAGTCCTGTTAAAAAATTGATATACACAGCTGAGAATGATGCTCCGTTGTTAGGACGGTACAACACCCTATCTCAAGAGCACACAGATGCAACAAGATTTAATTTGTTTACTGGAAATCAAACTCTAGATCAGAGAGATGAAATGTTTAAGGTAAATGAAACTGTTACTATAAACTGTGGTTTCTACAGTGAAAGTGGTGGGTTTAAGATCTCAAATGAAGACAAGAATTACATGAAAAGTTGTGACATTGTAGTCTCCACTTGTGCATTTGGTAGTGGAGATGATCTTTATCAACCCATTGGAATGTCAGAGGCATCCCCTCAGAAGGTTTGCTTTGTTGCTTTTTGGGATGAAGTTACTGTGAGTACTCAAGAGTCAGCTGAACATAGAATTGGTGAGGATGGATTTATTGGGAAATGGCGCATTGTGGTTGTAAGAGATCTCCCTTTTGCGGACCAAAGGTTAAATGGAAAAATCCCAAAGATGTTGGCGCATCGTCTTTTTCCTCATTCAAAGTATTCCATTTGGGTAGATTCGAAGTCCCAATTTAGGAGGGACCCTTTAGGTGTGCTCGAAGCTCTTCTCTGGCGTACAAATTCTGTACTTGCAATCTCAGAACATGGAGCTCGTAGTAGCATTTATGACGAGGCGAAGGCTGTTGTCAAGAAAAACAAGGCCAAACCTGAAGAAGTTGAGCTGCAGTTGACTCAATATCGGAGTGATGGCTTGCCAGAAGACAAGCAATTCAATGGAAAGAAAGCATTCCACTTCCAGTTCATATATGTTCAATCTGTGAAGTGGGTTATAGCCCTTGCCCGTAGTTTGCTGCCTCAGACATTTAAATTGACAACCAACAGTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616 Dirigent

Protein Analysis

416

Amino Acids

47.57

Weight (kDa)

8.72

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 155 - 382 9e-87 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 638
AccII CGCG 1 cut(s) 69
AciI CCGC 3 cut(s) 179, 257, 839
AclI AACGTT 1 cut(s) 319
AclWI GGATC 1 cut(s) 278
AcoI YGGCCR 2 cut(s) 58, 180
AcsI RAATTY 2 cut(s) 363, 982
AcuI CTGAAG 1 cut(s) 1092
AfaI GTAC 4 cut(s) 446, 754, 979, 990
AfiI CCNNNNNNNGG 6 cut(s) 156, 157, 437, 712, 838, 936
AgsI TTSAA 5 cut(s) 112, 274, 900, 1135, 1175
AhlI ACTAGT 1 cut(s) 386
AjuI GAANNNNNNNTTGG 2 cut(s) 762, 794
AleI CACNNNNGTG 1 cut(s) 257
AluBI AGCT 7 cut(s) 55, 141, 416, 767, 965, 1013, 1084
AluI AGCT 7 cut(s) 55, 141, 416, 767, 965, 1013, 1084
Alw21I GWGCWC 4 cut(s) 57, 468, 960, 1015
Alw26I GTCTC 1 cut(s) 646
AlwI GGATC 1 cut(s) 278
AlwNI CAGNNNCTG 1 cut(s) 144
AoxI GGCC 5 cut(s) 58, 146, 159, 180, 1062
ApaI GGGCCC 1 cut(s) 163
ApeKI GCWGC 2 cut(s) 1084, 1213
ApoI RAATTY 2 cut(s) 363, 982
Asp700I GAANNNNTTC 2 cut(s) 966, 1148
AspLEI GCGC 4 cut(s) 69, 90, 810, 880
AspS9I GGNCC 5 cut(s) 147, 159, 160, 841, 943
AsuHPI GGTGA 3 cut(s) 136, 271, 794
AsuII TTCGAA 1 cut(s) 923
AvaII GGWCC 2 cut(s) 841, 943
BaeGI GKGCMC 2 cut(s) 163, 375
BanII GRGCYC 3 cut(s) 57, 163, 1015
BbsI GAAGAC 2 cut(s) 612, 1128
Bbv12I GWGCWC 4 cut(s) 57, 468, 960, 1015
BbvI GCAGC 2 cut(s) 1071, 1200
BccI CCATC 2 cut(s) 782, 1103
BcoDI GTCTC 1 cut(s) 646
BcuI ACTAGT 1 cut(s) 386
BfaI CTAG 3 cut(s) 176, 387, 512
BfmI CTRYAG 2 cut(s) 571, 1085
BglII AGATCT 2 cut(s) 594, 826
BisI GCNGC 3 cut(s) 180, 1085, 1214
BlsI GCNGC 3 cut(s) 181, 1086, 1215
BmcAI AGTACT 1 cut(s) 754
Bme18I GGWCC 2 cut(s) 841, 943
BmgT120I GGNCC 5 cut(s) 147, 159, 160, 841, 943
BmiI GGNNCC 4 cut(s) 149, 161, 944, 945
BmsI GCATC 6 cut(s) 54, 364, 415, 463, 710, 889
BpiI GAAGAC 2 cut(s) 612, 1128
Bpu14I TTCGAA 1 cut(s) 923
BpuEI CTTGAG 3 cut(s) 371, 444, 741
BsaBI GATNNNNATC 2 cut(s) 273, 675
Bsc4I CCNNNNNNNGG 6 cut(s) 156, 157, 437, 712, 838, 936
Bse1I ACTGG 3 cut(s) 149, 502, 1159
Bse8I GATNNNNATC 2 cut(s) 273, 675
BseGI GGATG 5 cut(s) 43, 69, 701, 742, 793
BseJI GATNNNNATC 2 cut(s) 273, 675
BseLI CCNNNNNNNGG 6 cut(s) 156, 157, 437, 712, 838, 936
BseMII CTCAG 6 cut(s) 258, 308, 408, 722, 1014, 1232
BseNI ACTGG 3 cut(s) 149, 502, 1159
BseSI GKGCMC 2 cut(s) 163, 375
BseX3I CGGCCG 1 cut(s) 58
BseXI GCAGC 2 cut(s) 1071, 1200
Bsh1236I CGCG 1 cut(s) 69
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 5 cut(s) 60, 148, 161, 182, 1064
BsiEI CGRYCG 1 cut(s) 61
BsiHKAI GWGCWC 4 cut(s) 57, 468, 960, 1015
BsiSI CCGG 2 cut(s) 61, 82
BslFI GGGAC 2 cut(s) 913, 956
BslI CCNNNNNNNGG 6 cut(s) 156, 157, 437, 712, 838, 936
BsmAI GTCTC 1 cut(s) 646
BsmFI GGGAC 2 cut(s) 913, 956
BsmI GAATGC 1 cut(s) 1148
BsnI GGCC 5 cut(s) 60, 148, 161, 182, 1064
Bsp119I TTCGAA 1 cut(s) 923
Bsp120I GGGCCC 1 cut(s) 159
Bsp1286I GDGCHC 6 cut(s) 57, 163, 375, 468, 960, 1015
Bsp143I GATC 5 cut(s) 283, 514, 594, 670, 826
BspACI CCGC 3 cut(s) 179, 257, 839
BspANI GGCC 5 cut(s) 60, 148, 161, 182, 1064
BspCNI CTCAG 6 cut(s) 257, 307, 409, 721, 1013, 1231
BspFNI CGCG 1 cut(s) 69
BspLI GGNNCC 4 cut(s) 149, 161, 944, 945
BspMAI CTGCAG 1 cut(s) 1089
BspPI GGATC 1 cut(s) 278
BspQI GCTCTTC 1 cut(s) 972
BspT104I TTCGAA 1 cut(s) 923
BsrI ACTGG 3 cut(s) 149, 502, 1159
BssMI GATC 5 cut(s) 283, 514, 594, 670, 826
Bst4CI ACNGT 6 cut(s) 444, 550, 563, 575, 748, 1244
Bst6I CTCTTC 1 cut(s) 972
BstBI TTCGAA 1 cut(s) 923
BstC8I GCNNGC 2 cut(s) 326, 1115
BstDEI CTNAG 6 cut(s) 244, 294, 417, 708, 1000, 1218
BstF5I GGATG 5 cut(s) 43, 69, 701, 742, 793
BstFNI CGCG 1 cut(s) 69
BstHHI GCGC 4 cut(s) 69, 90, 810, 880
BstKTI GATC 5 cut(s) 286, 517, 597, 673, 829
BstMAI GTCTC 1 cut(s) 646
BstMBI GATC 5 cut(s) 283, 514, 594, 670, 826
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 2 cut(s) 66, 87
BstSFI CTRYAG 2 cut(s) 571, 1085
BstSLI GKGCMC 2 cut(s) 163, 375
BstUI CGCG 1 cut(s) 69
BstV1I GCAGC 2 cut(s) 1071, 1200
BstV2I GAAGAC 2 cut(s) 612, 1128
BstX2I RGATCY 2 cut(s) 594, 826
BstYI RGATCY 2 cut(s) 594, 826
BstZI CGGCCG 1 cut(s) 58
BsuRI GGCC 5 cut(s) 60, 148, 161, 182, 1064
BtsCI GGATG 5 cut(s) 43, 69, 701, 742, 793
BtsI GCAGTG 2 cut(s) 36, 252
BtsIMutI CAGTG 3 cut(s) 36, 252, 580
Cac8I GCNNGC 2 cut(s) 326, 1115
CaiI CAGNNNCTG 1 cut(s) 144
CfoI GCGC 4 cut(s) 69, 90, 810, 880
Cfr13I GGNCC 5 cut(s) 147, 159, 160, 841, 943
Csp6I GTAC 4 cut(s) 445, 753, 978, 989
CviAII CATG 3 cut(s) 207, 619, 1007
CviQI GTAC 4 cut(s) 445, 753, 978, 989
DdeI CTNAG 6 cut(s) 244, 294, 417, 708, 1000, 1218
DpnI GATC 5 cut(s) 285, 516, 596, 672, 828
DpnII GATC 5 cut(s) 283, 514, 594, 670, 826
DraI TTTAAA 1 cut(s) 1228
DrdI GACNNNNNNGTC 1 cut(s) 638
DseDI GACNNNNNNGTC 1 cut(s) 638
EaeI YGGCCR 2 cut(s) 58, 180
EagI CGGCCG 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 972
EarI CTCTTC 1 cut(s) 972
Ecl136II GAGCTC 2 cut(s) 55, 1013
EclXI CGGCCG 1 cut(s) 58
Eco24I GRGCYC 3 cut(s) 57, 163, 1015
Eco47I GGWCC 2 cut(s) 841, 943
Eco52I CGGCCG 1 cut(s) 58
Eco53kI GAGCTC 2 cut(s) 55, 1013
Eco57I CTGAAG 1 cut(s) 1092
EcoICRI GAGCTC 2 cut(s) 55, 1013
EcoO109I RGGNCCY 2 cut(s) 160, 943
EcoT38I GRGCYC 3 cut(s) 57, 163, 1015
FaeI CATG 3 cut(s) 210, 622, 1010
FalI AAGNNNNNCTT 2 cut(s) 704, 736
FaqI GGGAC 2 cut(s) 913, 956
FatI CATG 3 cut(s) 206, 618, 1006
Fnu4HI GCNGC 3 cut(s) 180, 1085, 1214
FokI GGATG 5 cut(s) 50, 76, 688, 749, 800
FriOI GRGCYC 3 cut(s) 57, 163, 1015
Fsp4HI GCNGC 3 cut(s) 180, 1085, 1214
FspBI CTAG 3 cut(s) 176, 387, 512
GlaI GCGC 4 cut(s) 68, 89, 809, 879
GluI GCNGC 3 cut(s) 180, 1085, 1214
HaeIII GGCC 5 cut(s) 60, 148, 161, 182, 1064
HapII CCGG 2 cut(s) 61, 82
HhaI GCGC 4 cut(s) 69, 90, 810, 880
Hin1II CATG 3 cut(s) 210, 622, 1010
Hin6I GCGC 4 cut(s) 67, 88, 808, 878
HinP1I GCGC 4 cut(s) 67, 88, 808, 878
HincII GTYRAC 1 cut(s) 1092
HindII GTYRAC 1 cut(s) 1092
HinfI GANTC 8 cut(s) 44, 108, 130, 274, 380, 761, 920, 1093
HpaII CCGG 2 cut(s) 61, 82
HphI GGTGA 3 cut(s) 136, 271, 794
Hpy166II GTNNAC 3 cut(s) 262, 495, 1092
Hpy188I TCNGA 9 cut(s) 37, 49, 247, 519, 697, 711, 1003, 1104, 1221
Hpy188III TCNNGA 7 cut(s) 112, 341, 359, 461, 512, 758, 1051
Hpy8I GTNNAC 3 cut(s) 262, 495, 1092
HpyAV CCTTC 2 cut(s) 706, 1033
HpyCH4III ACNGT 6 cut(s) 444, 550, 563, 575, 748, 1244
HpyCH4IV ACGT 1 cut(s) 319
HpyCH4V TGCA 4 cut(s) 476, 653, 995, 1087
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 87
HpyF3I CTNAG 6 cut(s) 244, 294, 417, 708, 1000, 1218
HpySE526I ACGT 1 cut(s) 319
Hsp92II CATG 3 cut(s) 210, 622, 1010
HspAI GCGC 4 cut(s) 67, 88, 808, 878
KflI GGGWCCC 1 cut(s) 943
Kzo9I GATC 5 cut(s) 283, 514, 594, 670, 826
LguI GCTCTTC 1 cut(s) 972
LmnI GCTCC 2 cut(s) 433, 1010
Lsp1109I GCAGC 2 cut(s) 1071, 1200
LweI GCATC 6 cut(s) 54, 364, 415, 463, 710, 889
MaeI CTAG 3 cut(s) 176, 387, 512
MaeII ACGT 1 cut(s) 319
MaeIII GTNAC 4 cut(s) 310, 550, 629, 742
MalI GATC 5 cut(s) 285, 516, 596, 672, 828
MboI GATC 5 cut(s) 283, 514, 594, 670, 826
MboII GAAGA 4 cut(s) 617, 959, 1085, 1133
MflI RGATCY 2 cut(s) 594, 826
MhlI GDGCHC 6 cut(s) 57, 163, 375, 468, 960, 1015
MlyI GAGTC 2 cut(s) 770, 1087
MmeI TCCRAC 1 cut(s) 15
MroXI GAANNNNTTC 2 cut(s) 966, 1148
MseI TTAA 6 cut(s) 396, 486, 534, 591, 851, 1227
MslI CAYNNNNRTG 4 cut(s) 23, 257, 471, 689
MspA1I CMGCKG 2 cut(s) 416, 767
MspI CCGG 2 cut(s) 61, 82
Mva1269I GAATGC 1 cut(s) 1148
MvnI CGCG 1 cut(s) 69
MwoI GCNNNNNNNGC 2 cut(s) 66, 87
NdeII GATC 5 cut(s) 283, 514, 594, 670, 826
NlaIII CATG 3 cut(s) 210, 622, 1010
NlaIV GGNNCC 4 cut(s) 149, 161, 944, 945
NmeAIII GCCGAG 1 cut(s) 36
NmuCI GTSAC 1 cut(s) 629
NspV TTCGAA 1 cut(s) 923
OliI CACNNNNGTG 1 cut(s) 257
PciSI GCTCTTC 1 cut(s) 972
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PctI GAATGC 1 cut(s) 1148
PdmI GAANNNNTTC 2 cut(s) 966, 1148
PfeI GAWTC 6 cut(s) 44, 108, 130, 274, 380, 920
PkrI GCNGC 3 cut(s) 181, 1086, 1215
PleI GAGTC 2 cut(s) 769, 1087
PpsI GAGTC 2 cut(s) 769, 1087
PpuMI RGGWCCY 1 cut(s) 943
Psp124BI GAGCTC 2 cut(s) 57, 1015
Psp1406I AACGTT 1 cut(s) 319
Psp5II RGGWCCY 1 cut(s) 943
PspN4I GGNNCC 4 cut(s) 149, 161, 944, 945
PspOMI GGGCCC 1 cut(s) 159
PspPI GGNCC 5 cut(s) 147, 159, 160, 841, 943
PspPPI RGGWCCY 1 cut(s) 943
PstI CTGCAG 1 cut(s) 1089
PstNI CAGNNNCTG 1 cut(s) 144
PsuI RGATCY 2 cut(s) 594, 826
PvuII CAGCTG 2 cut(s) 416, 767
RsaI GTAC 4 cut(s) 446, 754, 979, 990
RsaNI GTAC 4 cut(s) 445, 753, 978, 989
RseI CAYNNNNRTG 4 cut(s) 23, 257, 471, 689
SacI GAGCTC 2 cut(s) 57, 1015
SapI GCTCTTC 1 cut(s) 972
SaqAI TTAA 6 cut(s) 396, 486, 534, 591, 851, 1227
SatI GCNGC 3 cut(s) 180, 1085, 1214
Sau3AI GATC 5 cut(s) 283, 514, 594, 670, 826
Sau96I GGNCC 5 cut(s) 147, 159, 160, 841, 943
ScaI AGTACT 1 cut(s) 754
SchI GAGTC 2 cut(s) 770, 1087
SduI GDGCHC 6 cut(s) 57, 163, 375, 468, 960, 1015
SfaNI GCATC 6 cut(s) 54, 364, 415, 463, 710, 889
SfcI CTRYAG 2 cut(s) 571, 1085
SfuI TTCGAA 1 cut(s) 923
SinI GGWCC 2 cut(s) 841, 943
SmiI ATTTAAAT 1 cut(s) 1228
SmiMI CAYNNNNRTG 4 cut(s) 23, 257, 471, 689
SmlI CTYRAG 3 cut(s) 350, 459, 756
SmoI CTYRAG 3 cut(s) 350, 459, 756
SpeI ACTAGT 1 cut(s) 386
SsiI CCGC 3 cut(s) 179, 257, 839
SspMI CTAG 3 cut(s) 176, 387, 512
SstI GAGCTC 2 cut(s) 57, 1015
SwaI ATTTAAAT 1 cut(s) 1228
TaaI ACNGT 6 cut(s) 444, 550, 563, 575, 748, 1244
TaiI ACGT 1 cut(s) 322
TaqI TCGA 3 cut(s) 73, 923, 960
TatI WGTACW 2 cut(s) 752, 988
TauI GCSGC 1 cut(s) 182
TfiI GAWTC 6 cut(s) 44, 108, 130, 274, 380, 920
Tru1I TTAA 6 cut(s) 396, 486, 534, 591, 851, 1227
Tru9I TTAA 6 cut(s) 396, 486, 534, 591, 851, 1227
TscAI CASTG 3 cut(s) 36, 259, 580
TseFI GTSAC 1 cut(s) 629
TseI GCWGC 2 cut(s) 1084, 1213
Tsp45I GTSAC 1 cut(s) 629
TspDTI ATGAA 7 cut(s) 20, 540, 558, 618, 635, 753, 1153
TspGWI ACGGA 2 cut(s) 132, 420
TspRI CASTG 3 cut(s) 36, 259, 580
VpaK11BI GGWCC 2 cut(s) 841, 943
XapI RAATTY 2 cut(s) 363, 982
XbaI TCTAGA 1 cut(s) 511
XcmI CCANNNNNNNNNTGG 1 cut(s) 851
XmnI GAANNNNTTC 2 cut(s) 966, 1148
XspI CTAG 3 cut(s) 176, 387, 512
ZrmI AGTACT 1 cut(s) 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.