Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0002116

Physical Location & Seq

Genomic Coordinates Forward (+)

3198378 .. 3205198

Gene Structure 6821 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1040400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1371 bp

ATGGGTGGTGATGAAGCAGCAGAAGATCAGCAGCTACCGTTGCTCCAACCTCTCAAAAGAACAGGAACCATATGGACTGCAGTAGCACATATTATAACAGGCGTGATAGGAGCAGGAGTATTATCTCTTGCATGGAGTGTGGCTCAGCTGGGGTGGATTGCAGGTCCATTGCTTATGATTGTCTTTGCTTCAGTCACCATTCTTTCCAACAACCTCCTCTCCGACTGCTACCGATTCCCGGAATCCGATAGCCCGACGAGAGTCGGATCCTACATGGAAGCTGTCAAGGTCTACCTAGGAGAGAAGAGTAAAACAGTGTGTGCAATATTTGTACACGAGAGCTTGTATGGCTGTGGAATCGCTTACGTTATTACAGCTGCTAGCAGCGTCAAAGCCATTCAGAGATCAAACTGCTATCACAAAGAAGGGCATGAAGCTTCCTGTCAATATGGGACTGGTTTGTACATGATGCTATTTGGACTTGTCCAGATTGTAGTGTCACAAATACCAGATTTCCACAACATGGAATGGCTATCAGTTGTTGCAGCAATCATGTCGTTTACCTACTCTTTCATTGGACTTGGCCTCGGCATGGCGAAAGCAATAGAAAATGGGAGGATTCAAGGAAGCCTGGCAGGAGTGCCAACCTCTAATGTTGCTGATAAATTATGGTTAGTCTTCCAAGCACTTGGAGACATCGCTTTTGCCTATCCGTACTCTATTATTCTTCTTGAAATTCAGGATACTTTGAAGTCCCCTCCACCAGAAAATCAGACAATGAAGAAGGCCTCAATGATTGCCATATTTGTCACAACCTTCTTCTACCTCTGCTGTGGATGCTTTGGATACGCCGCCTTTGGGAATGACACGCCCGGGAACCTCTTGACAGGATTCGGATTCTACGAACCCTACTGGCTCATTGATTTTGCTAATGCTTGCATTGTTCTTCATTTGGTGGGAGGATATCAGGTCTACAGTCAACCAGTATTTGCAGTTGCTGAGAGATGGTCCAGGAAGAAATACCCAAACAGCGGATTTGTGAACAACTTTTACTCCATCAAAATCCCATTGTTGCCAAGATTTCAAGTGAATCCCTTCAGGCTGTGTTTCCGAACTGTTTACGTTGTATCAACTACCGCAATTGCGATGATATTTCCTTACTTCAACCAGGTTTTGGGAGTTTTAGGGGCCTTAAACTTTTGGCCCTTGGCTATATATTTCCCTGTTGAAATGTACTTTGTACAAAAGAGAATCGGGGCTTGGACAAAAAAATGGATTGTTCTAAGGATATTTAGTTTCGTTTGCTTTCTTGTGACGGTTGTGGGTGTGATTGGATCAGTTGAAGGACTTATTAGTGCCAAACTTAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003333 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006865 GO:0008150 GO:0015711 GO:0015849 GO:0016020 GO:0034220 GO:0044464 GO:0046942 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071944 GO:0098656 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Aa_trans

Protein Analysis

457

Amino Acids

50.32

Weight (kDa)

8.38

Isoelectric Point (pI)

30.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aa_trans	PF01490	20 - 451	3.7e-100	Transmembrane amino acid transporter protein
Trp_Tyr_perm	PF03222	23 - 193	1.2e-06	Tryptophan/tyrosine permease family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002116)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23810 AT5G23810 AT5G23810 AT5G23810 AT5G23810
fragaria_vesca	FvH4_3g41850 FvH4_3g41850 FvH4_3g41850
malus_domestica	MD03G1040400.v1.1 MD11G1041800.v1.1
prunus_persica	Prupe.6G032700_v2.0.a1 Prupe.6G032700_v2.0.a1 Prupe.6G032700_v2.0.a1 Prupe.6G032700_v2.0.a1 Prupe.6G032700_v2.0.a1 Prupe.6G032700_v2.0.a1 Prupe.6G032700_v2.0.a1
pyrus_communis	pycom03g03110 pycom03g03120 pycom08g18190 pycom11g03550 pycom11g03570 pycom11g03580
rosa_chinensis	RchiOBHm_Chr5g0075041 RchiOBHm_Chr5g0075051
rosa_laevigata	RLG00000036520 RLG00000036521
rosa_multiflora	Rmu_co8358743.1_g000001 Rmu_sc0006984.1_g000004 Rmu_sc0006984.1_g000012
rosa_roxburghii	Rroxscaffold_1G00006590 Rroxscaffold_1G00006610
rosa_rugosa	Rorug05G0435700
rosa_samantha	Rh5AG493000 Rh5AG493100 Rh5BG514300 Rh5BG514600 Rh5CG537900 Rh5CG538200 Rh5DG526600 Rh5DG526700 Rh5DG526900
rosa_wichuraiana	Rw5G045700 Rw5G045730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	95
Acc36I	ACCTGC	1 cut(s)	152
AccB7I	CCANNNNNTGG	2 cut(s)	523, 1174
AccI	GTMKAC	2 cut(s)	291, 972
AciI	CCGC	3 cut(s)	852, 1032, 1137
AclWI	GGATC	3 cut(s)	261, 274, 1342
AcsI	RAATTY	1 cut(s)	735
AcuI	CTGAAG	2 cut(s)	174, 1081
AfaI	GTAC	5 cut(s)	333, 464, 716, 1235, 1242
AfiI	CCNNNNNNNGG	6 cut(s)	238, 523, 592, 858, 1031, 1174
AgsI	TTSAA	7 cut(s)	623, 734, 751, 1085, 1165, 1229, 1343
AjnI	CCWGG	3 cut(s)	630, 1010, 1167
AjuI	GAANNNNNNNTTGG	2 cut(s)	1243, 1275
AluBI	AGCT	7 cut(s)	34, 148, 281, 342, 377, 437, 1368
AluI	AGCT	7 cut(s)	34, 148, 281, 342, 377, 437, 1368
Alw26I	GTCTC	1 cut(s)	687
AlwI	GGATC	3 cut(s)	261, 274, 1342
AlwNI	CAGNNNCTG	1 cut(s)	998
Ama87I	CYCGRG	1 cut(s)	872
AoxI	GGCC	4 cut(s)	583, 786, 1188, 1202
ApeKI	GCWGC	5 cut(s)	17, 31, 377, 384, 545
ApoI	RAATTY	1 cut(s)	735
ArsI	GACNNNNNNTTYG	2 cut(s)	686, 718
Asp700I	GAANNNNTTC	1 cut(s)	1094
AspA2I	CCTAGG	1 cut(s)	295
AspS9I	GGNCC	4 cut(s)	164, 1008, 1188, 1203
AsuC2I	CCSGG	3 cut(s)	239, 873, 874
AsuHPI	GGTGA	2 cut(s)	20, 187
AsuNHI	GCTAGC	1 cut(s)	380
AvaI	CYCGRG	1 cut(s)	872
AvaII	GGWCC	2 cut(s)	164, 1008
AvrII	CCTAGG	1 cut(s)	295
BamHI	GGATCC	1 cut(s)	266
BauI	CACGAG	1 cut(s)	335
BbsI	GAAGAC	1 cut(s)	670
BbvI	GCAGC	5 cut(s)	29, 43, 364, 396, 557
BccI	CCATC	2 cut(s)	999, 1064
BcgI	CGANNNNNNTGC	2 cut(s)	537, 571
BciT130I	CCWGG	3 cut(s)	632, 1012, 1169
BciVI	GTATCC	2 cut(s)	736, 839
BcnI	CCSGG	3 cut(s)	239, 873, 874
BcoDI	GTCTC	1 cut(s)	687
BfaI	CTAG	3 cut(s)	296, 381, 1369
BfmI	CTRYAG	2 cut(s)	78, 973
BfuAI	ACCTGC	1 cut(s)	152
BfuI	GTATCC	2 cut(s)	736, 839
BisI	GCNGC	6 cut(s)	18, 32, 378, 385, 546, 852
BlnI	CCTAGG	1 cut(s)	295
BlpI	GCTNAGC	1 cut(s)	144
BlsI	GCNGC	6 cut(s)	19, 33, 379, 386, 547, 853
Bme1390I	CCNGG	6 cut(s)	239, 632, 873, 874, 1012, 1169
Bme18I	GGWCC	2 cut(s)	164, 1008
BmeT110I	CYCGRG	1 cut(s)	872
BmgT120I	GGNCC	4 cut(s)	164, 1008, 1188, 1203
BmiI	GGNNCC	4 cut(s)	67, 268, 878, 1189
BmrFI	CCNGG	6 cut(s)	239, 632, 873, 874, 1012, 1169
BmsI	GCATC	2 cut(s)	459, 827
BmtI	GCTAGC	1 cut(s)	384
BoxI	GACNNNNGTC	1 cut(s)	260
BpiI	GAAGAC	1 cut(s)	670
BplI	GAGNNNNNCTC	2 cut(s)	127, 159
Bpu1102I	GCTNAGC	1 cut(s)	144
BpuMI	CCSGG	3 cut(s)	239, 873, 874
BsaJI	CCNNGG	4 cut(s)	295, 586, 872, 1206
BsaXI	ACNNNNNCTCC	8 cut(s)	27, 57, 102, 132, 203, 233, 1037, 1067
Bsc4I	CCNNNNNNNGG	6 cut(s)	238, 523, 592, 858, 1031, 1174
Bse1I	ACTGG	3 cut(s)	460, 917, 983
Bse3DI	GCAATG	1 cut(s)	167
BseBI	CCWGG	3 cut(s)	632, 1012, 1169
BseDI	CCNNGG	4 cut(s)	295, 586, 872, 1206
BseGI	GGATG	1 cut(s)	842
BseLI	CCNNNNNNNGG	6 cut(s)	238, 523, 592, 858, 1031, 1174
BseMI	GCAATG	1 cut(s)	167
BseMII	CTCAG	2 cut(s)	158, 990
BseNI	ACTGG	3 cut(s)	460, 917, 983
BseRI	GAGGAG	1 cut(s)	206
BseXI	GCAGC	5 cut(s)	29, 43, 364, 396, 557
BseYI	CCCAGC	1 cut(s)	148
BshFI	GGCC	4 cut(s)	585, 788, 1190, 1204
BsiHKCI	CYCGRG	1 cut(s)	872
BsiSI	CCGG	2 cut(s)	239, 873
BslFI	GGGAC	2 cut(s)	466, 739
BslI	CCNNNNNNNGG	6 cut(s)	238, 523, 592, 858, 1031, 1174
BsmAI	GTCTC	1 cut(s)	687
BsmFI	GGGAC	2 cut(s)	466, 739
BsnI	GGCC	4 cut(s)	585, 788, 1190, 1204
BsoBI	CYCGRG	1 cut(s)	872
Bsp1407I	TGTACA	3 cut(s)	331, 462, 1240
Bsp143I	GATC	4 cut(s)	25, 266, 404, 1334
Bsp1720I	GCTNAGC	1 cut(s)	144
BspACI	CCGC	3 cut(s)	852, 1032, 1137
BspANI	GGCC	4 cut(s)	585, 788, 1190, 1204
BspCNI	CTCAG	2 cut(s)	157, 991
BspLI	GGNNCC	4 cut(s)	67, 268, 878, 1189
BspMAI	CTGCAG	1 cut(s)	82
BspMI	ACCTGC	1 cut(s)	152
BspOI	GCTAGC	1 cut(s)	384
BspPI	GGATC	3 cut(s)	261, 274, 1342
BsrDI	GCAATG	1 cut(s)	167
BsrGI	TGTACA	3 cut(s)	331, 462, 1240
BsrI	ACTGG	3 cut(s)	460, 917, 983
BssECI	CCNNGG	4 cut(s)	295, 586, 872, 1206
BssMI	GATC	4 cut(s)	25, 266, 404, 1334
BssSI	CACGAG	1 cut(s)	335
BssT1I	CCWWGG	2 cut(s)	295, 1206
Bst2BI	CACGAG	1 cut(s)	335
Bst2UI	CCWGG	3 cut(s)	632, 1012, 1169
Bst4CI	ACNGT	5 cut(s)	39, 316, 977, 1117, 1318
Bst6I	CTCTTC	1 cut(s)	299
BstAUI	TGTACA	3 cut(s)	331, 462, 1240
BstC8I	GCNNGC	2 cut(s)	382, 937
BstDEI	CTNAG	4 cut(s)	144, 999, 1283, 1364
BstF5I	GGATG	1 cut(s)	842
BstKTI	GATC	4 cut(s)	28, 269, 407, 1337
BstMAI	GTCTC	1 cut(s)	687
BstMBI	GATC	4 cut(s)	25, 266, 404, 1334
BstMWI	GCNNNNNNNGC	3 cut(s)	40, 348, 837
BstNI	CCWGG	3 cut(s)	632, 1012, 1169
BstPAI	GACNNNNGTC	1 cut(s)	260
BstSCI	CCNGG	6 cut(s)	237, 630, 871, 872, 1010, 1167
BstSFI	CTRYAG	2 cut(s)	78, 973
BstV1I	GCAGC	5 cut(s)	29, 43, 364, 396, 557
BstV2I	GAAGAC	1 cut(s)	670
BstX2I	RGATCY	1 cut(s)	266
BstXI	CCANNNNNNTGG	1 cut(s)	689
BstYI	RGATCY	1 cut(s)	266
BsuI	GTATCC	2 cut(s)	736, 839
BsuRI	GGCC	4 cut(s)	585, 788, 1190, 1204
BtgZI	GCGATG	2 cut(s)	682, 1160
BtsCI	GGATG	1 cut(s)	842
BtsIMutI	CAGTG	1 cut(s)	321
BveI	ACCTGC	1 cut(s)	152
Cac8I	GCNNGC	2 cut(s)	382, 937
CaiI	CAGNNNCTG	1 cut(s)	998
Cfr13I	GGNCC	4 cut(s)	164, 1008, 1188, 1203
Cfr9I	CCCGGG	1 cut(s)	872
CseI	GACGC	1 cut(s)	376
CsiI	ACCWGGT	1 cut(s)	1167
Csp6I	GTAC	5 cut(s)	332, 463, 715, 1234, 1241
CviAII	CATG	7 cut(s)	132, 274, 431, 466, 523, 553, 592
CviQI	GTAC	5 cut(s)	332, 463, 715, 1234, 1241
DdeI	CTNAG	4 cut(s)	144, 999, 1283, 1364
DpnI	GATC	4 cut(s)	27, 268, 406, 1336
DpnII	GATC	4 cut(s)	25, 266, 404, 1334
Eam1104I	CTCTTC	1 cut(s)	299
EarI	CTCTTC	1 cut(s)	299
Eco130I	CCWWGG	2 cut(s)	295, 1206
Eco147I	AGGCCT	1 cut(s)	788
Eco32I	GATATC	1 cut(s)	965
Eco47I	GGWCC	2 cut(s)	164, 1008
Eco57I	CTGAAG	2 cut(s)	174, 1081
Eco88I	CYCGRG	1 cut(s)	872
EcoO109I	RGGNCCY	1 cut(s)	1188
EcoRII	CCWGG	3 cut(s)	630, 1010, 1167
EcoRV	GATATC	1 cut(s)	965
EcoT14I	CCWWGG	2 cut(s)	295, 1206
ErhI	CCWWGG	2 cut(s)	295, 1206
FaeI	CATG	7 cut(s)	135, 277, 434, 469, 526, 556, 595
FaqI	GGGAC	2 cut(s)	466, 739
FatI	CATG	7 cut(s)	131, 273, 430, 465, 522, 552, 591
FauNDI	CATATG	1 cut(s)	71
FblI	GTMKAC	2 cut(s)	291, 972
Fnu4HI	GCNGC	6 cut(s)	18, 32, 378, 385, 546, 852
FokI	GGATG	1 cut(s)	849
Fsp4HI	GCNGC	6 cut(s)	18, 32, 378, 385, 546, 852
FspBI	CTAG	3 cut(s)	296, 381, 1369
GluI	GCNGC	6 cut(s)	18, 32, 378, 385, 546, 852
GsaI	CCCAGC	1 cut(s)	152
HaeIII	GGCC	4 cut(s)	585, 788, 1190, 1204
HapII	CCGG	2 cut(s)	239, 873
HgaI	GACGC	1 cut(s)	376
Hin1II	CATG	7 cut(s)	135, 277, 434, 469, 526, 556, 595
HincII	GTYRAC	1 cut(s)	980
HindII	GTYRAC	1 cut(s)	980
HindIII	AAGCTT	1 cut(s)	435
HinfI	GANTC	9 cut(s)	234, 242, 261, 357, 619, 891, 897, 1090, 1251
HpaII	CCGG	2 cut(s)	239, 873
HphI	GGTGA	2 cut(s)	20, 187
Hpy166II	GTNNAC	7 cut(s)	292, 334, 561, 973, 980, 1042, 1120
Hpy188I	TCNGA	7 cut(s)	223, 247, 266, 402, 774, 896, 1112
Hpy188III	TCNNGA	4 cut(s)	487, 731, 740, 883
Hpy8I	GTNNAC	7 cut(s)	292, 334, 561, 973, 980, 1042, 1120
Hpy99I	CGWCG	1 cut(s)	259
HpyAV	CCTTC	5 cut(s)	419, 778, 826, 1105, 1337
HpyCH4III	ACNGT	5 cut(s)	39, 316, 977, 1117, 1318
HpyCH4IV	ACGT	2 cut(s)	366, 1122
HpyCH4V	TGCA	7 cut(s)	80, 131, 161, 323, 545, 939, 992
HpyF10VI	GCNNNNNNNGC	3 cut(s)	40, 348, 837
HpyF3I	CTNAG	4 cut(s)	144, 999, 1283, 1364
HpySE526I	ACGT	2 cut(s)	366, 1122
Hsp92II	CATG	7 cut(s)	135, 277, 434, 469, 526, 556, 595
Kzo9I	GATC	4 cut(s)	25, 266, 404, 1334
LmnI	GCTCC	2 cut(s)	48, 110
Lsp1109I	GCAGC	5 cut(s)	29, 43, 364, 396, 557
LweI	GCATC	2 cut(s)	459, 827
MabI	ACCWGGT	1 cut(s)	1167
MaeI	CTAG	3 cut(s)	296, 381, 1369
MaeII	ACGT	2 cut(s)	366, 1122
MaeIII	GTNAC	4 cut(s)	193, 498, 808, 1312
MalI	GATC	4 cut(s)	27, 268, 406, 1336
MboI	GATC	4 cut(s)	25, 266, 404, 1334
MboII	GAAGA	8 cut(s)	35, 316, 670, 719, 793, 811, 938, 1027
MfeI	CAATTG	1 cut(s)	1140
MflI	RGATCY	1 cut(s)	266
MluCI	AATT	3 cut(s)	665, 735, 1140
MlyI	GAGTC	1 cut(s)	270
MmeI	TCCRAC	4 cut(s)	70, 231, 244, 246
MroXI	GAANNNNTTC	1 cut(s)	1094
MseI	TTAA	1 cut(s)	1193
MspA1I	CMGCKG	3 cut(s)	148, 377, 1032
MspI	CCGG	2 cut(s)	239, 873
MspR9I	CCNGG	6 cut(s)	239, 632, 873, 874, 1012, 1169
MunI	CAATTG	1 cut(s)	1140
MvaI	CCWGG	3 cut(s)	632, 1012, 1169
MwoI	GCNNNNNNNGC	3 cut(s)	40, 348, 837
NciI	CCSGG	3 cut(s)	239, 873, 874
NdeI	CATATG	1 cut(s)	71
NdeII	GATC	4 cut(s)	25, 266, 404, 1334
NheI	GCTAGC	1 cut(s)	380
NlaIII	CATG	7 cut(s)	135, 277, 434, 469, 526, 556, 595
NlaIV	GGNNCC	4 cut(s)	67, 268, 878, 1189
NmeAIII	GCCGAG	1 cut(s)	567
NmuCI	GTSAC	4 cut(s)	193, 498, 808, 1312
PceI	AGGCCT	1 cut(s)	788
PcsI	WCGNNNNNNNCGW	1 cut(s)	900
PdmI	GAANNNNTTC	1 cut(s)	1094
PfeI	GAWTC	8 cut(s)	234, 242, 357, 619, 891, 897, 1090, 1251
PflMI	CCANNNNNTGG	2 cut(s)	523, 1174
PfoI	TCCNGGA	2 cut(s)	237, 1010
PkrI	GCNGC	6 cut(s)	19, 33, 379, 386, 547, 853
PleI	GAGTC	1 cut(s)	269
PpsI	GAGTC	1 cut(s)	269
PshAI	GACNNNNGTC	1 cut(s)	260
PsiI	TTATAA	1 cut(s)	95
Psp6I	CCWGG	3 cut(s)	630, 1010, 1167
PspFI	CCCAGC	1 cut(s)	148
PspGI	CCWGG	3 cut(s)	630, 1010, 1167
PspN4I	GGNNCC	4 cut(s)	67, 268, 878, 1189
PspPI	GGNCC	4 cut(s)	164, 1008, 1188, 1203
PsrI	GAACNNNNNNTAC	2 cut(s)	1034, 1066
PstI	CTGCAG	1 cut(s)	82
PstNI	CAGNNNCTG	1 cut(s)	998
PsuI	RGATCY	1 cut(s)	266
PvuII	CAGCTG	2 cut(s)	148, 377
RsaI	GTAC	5 cut(s)	333, 464, 716, 1235, 1242
RsaNI	GTAC	5 cut(s)	332, 463, 715, 1234, 1241
SaqAI	TTAA	1 cut(s)	1193
SatI	GCNGC	6 cut(s)	18, 32, 378, 385, 546, 852
Sau3AI	GATC	4 cut(s)	25, 266, 404, 1334
Sau96I	GGNCC	4 cut(s)	164, 1008, 1188, 1203
SchI	GAGTC	1 cut(s)	270
ScrFI	CCNGG	6 cut(s)	239, 632, 873, 874, 1012, 1169
SexAI	ACCWGGT	1 cut(s)	1167
SfaNI	GCATC	2 cut(s)	459, 827
SfcI	CTRYAG	2 cut(s)	78, 973
SinI	GGWCC	2 cut(s)	164, 1008
SmaI	CCCGGG	1 cut(s)	874
Sse9I	AATT	3 cut(s)	665, 735, 1140
SseBI	AGGCCT	1 cut(s)	788
SsiI	CCGC	3 cut(s)	852, 1032, 1137
SspI	AATATT	1 cut(s)	327
SspMI	CTAG	3 cut(s)	296, 381, 1369
StuI	AGGCCT	1 cut(s)	788
StyD4I	CCNGG	6 cut(s)	237, 630, 871, 872, 1010, 1167
StyI	CCWWGG	2 cut(s)	295, 1206
TaaI	ACNGT	5 cut(s)	39, 316, 977, 1117, 1318
TaiI	ACGT	2 cut(s)	369, 1125
TasI	AATT	3 cut(s)	665, 735, 1140
TatI	WGTACW	4 cut(s)	331, 462, 1233, 1240
TauI	GCSGC	1 cut(s)	854
TfiI	GAWTC	8 cut(s)	234, 242, 357, 619, 891, 897, 1090, 1251
Tru1I	TTAA	1 cut(s)	1193
Tru9I	TTAA	1 cut(s)	1193
TscAI	CASTG	1 cut(s)	321
TseFI	GTSAC	4 cut(s)	193, 498, 808, 1312
TseI	GCWGC	5 cut(s)	17, 31, 377, 384, 545
Tsp45I	GTSAC	4 cut(s)	193, 498, 808, 1312
TspDTI	ATGAA	5 cut(s)	27, 447, 562, 794, 938
TspGWI	ACGGA	1 cut(s)	702
TspMI	CCCGGG	1 cut(s)	872
TspRI	CASTG	1 cut(s)	321
Van91I	CCANNNNNTGG	2 cut(s)	523, 1174
VpaK11BI	GGWCC	2 cut(s)	164, 1008
XapI	RAATTY	1 cut(s)	735
XmaI	CCCGGG	1 cut(s)	872
XmaJI	CCTAGG	1 cut(s)	295
XmiI	GTMKAC	2 cut(s)	291, 972
XmnI	GAANNNNTTC	1 cut(s)	1094
XspI	CTAG	3 cut(s)	296, 381, 1369

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.