Rh5DG526600

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002116
Physical Location & Seq
Reverse (-)
81415796 .. 81420643
4848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG526600.1

Sequence Viewer

Length: 1302 bp
ATGTGGACGGCAATAGCACATATAATAACAGGAGTAATAGGGGCAGGGGTGTTATCCCTGGCCTGGAGTGTGGCTCAGCTAGGGTGGATTGCAGGTCCATTGCTCATGATAGTCTTTGCTTCGGTCACCATCGTCTCCAACAACATTCTCTCTGACTGCTACAGATACCCGGATCCATATGCCCCGACCCGAAACAGGTCCTATTTGGAAGCTGTGAAGCTGTACTTAGGAGACAAGCTGCAAATAGTGACTGCCGTATTTGTACATGAGAGCTTGTATGGCAGTGGAATTGCCTATACCATTACAGCTGCTGGCAGTGTCAAAGCGATTTTGAAGTCAAACTGTTATCACAAGGAAGGGCATCAGGCTTCATGTACATATGGGGATACTCTGTATATGCTGATATTTGGAGCCGTACAGATTGTTGTGTCACAAATACCAGATTTCCATAACATGAAATGGCTTTCGGTTATTGCAGCAATCATGTCCTTTGCCTACTCTTTTATTGGACTTGGCCTCGGCTTTGCCAAAGTAATAGAAAATGGGAGTATTAAAGGGAGCTTGTCAGGAGTTGCAACCTCTAGTTTTGCTGCTAAATTATGGTTAATCTTCCAAGCAATTGGGGACATTGCTTTTGCCTATCCATACTCTATTATTCTTTTTGAGATTCAGGATACGTTAAAGTCCCCTCCAGCAGAAAACTACACGATGAAGAGGGCCTCTGTGATTGCCATCATTGTCACAACTTTCTTCTACCTCTGCTGCGGTTGCTTTGGATATGCAGCCTTTGGGGATGACACGCCGGGGAATCTCTTGACCGGATTTGGGTTTTACGAGCCTTACTGGCTCATTGATTTTGCTAATGCTTGCATAGTTCTTCATTTGGTGGGAGGATATCAGGTCTATAGTCAGCCAGTATTTGGAGTTGCTGAGAGATCGTTTAGCAAGAAGTATCCAAACAGTGCATTTGTGAGCAACTTTTACAGCTTCAAAATCCCAATGTTTGCGAGATTTCAAGTGAATCCCTTCAGGCTATGTTTCCGGACTGTTTATGTCATTTCAACTACAGTAATTGCAATCCTCTTTCCTTATTTCAACCAAGTTTTGGGAGTGTTAGGCGCCTTGAACTTTTGGCCCTTGGTTATATTTTTTCCTGTAGAGATGTACTTTGTACAAAAGAAAATTGGGACATGGACCAAGAAATGGATAGTATTACAGGCATTCAGTTTTGTTTGCTTTCTTGTGACTGTTATGGGTGTCATTGGATCAGTTGAAGGACTTATAAAAGCCAAACTTAGCTAG

Protein Analysis

433

Amino Acids

47.84

Weight (kDa)

8.88

Isoelectric Point (pI)

25.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 1 - 428 7.1e-99 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1283
Acc36I ACCTGC 1 cut(s) 83
AccB1I GGYRCC 1 cut(s) 1118
AccB7I CCANNNNNTGG 3 cut(s) 920, 1105, 1203
AccIII TCCGGA 1 cut(s) 1041
AciI CCGC 1 cut(s) 765
AclWI GGATC 3 cut(s) 167, 180, 1273
AcuI CTGAAG 1 cut(s) 1012
AcyI GRCGYC 1 cut(s) 1119
AfaI GTAC 6 cut(s) 224, 264, 376, 417, 1166, 1173
AfiI CCNNNNNNNGG 6 cut(s) 63, 195, 825, 920, 1105, 1203
AgsI TTSAA 7 cut(s) 334, 991, 1016, 1062, 1096, 1126, 1274
AjnI CCWGG 2 cut(s) 57, 62
AluBI AGCT 9 cut(s) 79, 212, 220, 238, 273, 308, 561, 987, 1299
AluI AGCT 9 cut(s) 79, 212, 220, 238, 273, 308, 561, 987, 1299
Alw26I GTCTC 2 cut(s) 139, 225
AlwI GGATC 3 cut(s) 167, 180, 1273
AlwNI CAGNNNCTG 1 cut(s) 311
Aor13HI TCCGGA 1 cut(s) 1041
AoxI GGCC 4 cut(s) 60, 514, 717, 1133
ApeKI GCWGC 6 cut(s) 238, 308, 476, 590, 762, 782
ArsI GACNNNNNNTTYG 2 cut(s) 617, 649
Asp700I GAANNNNTTC 1 cut(s) 1025
AspLEI GCGC 1 cut(s) 1121
AspS9I GGNCC 5 cut(s) 95, 198, 717, 1134, 1194
AsuC2I CCSGG 2 cut(s) 170, 804
AsuHPI GGTGA 1 cut(s) 118
AvaII GGWCC 3 cut(s) 95, 198, 1194
BamHI GGATCC 1 cut(s) 172
BanI GGYRCC 1 cut(s) 1118
BbvI GCAGC 6 cut(s) 225, 295, 488, 577, 749, 794
BccI CCATC 2 cut(s) 137, 740
BceAI ACGGC 3 cut(s) 24, 239, 398
BciT130I CCWGG 2 cut(s) 59, 64
BciVI GTATCC 3 cut(s) 379, 667, 963
BcnI CCSGG 2 cut(s) 170, 804
BcoDI GTCTC 2 cut(s) 139, 225
BfaI CTAG 3 cut(s) 80, 582, 1300
BfmI CTRYAG 4 cut(s) 160, 904, 1065, 1155
BfoI RGCGCY 1 cut(s) 1122
BfuAI ACCTGC 1 cut(s) 83
BfuI GTATCC 3 cut(s) 379, 667, 963
BglI GCCNNNNNGGC 1 cut(s) 844
BisI GCNGC 6 cut(s) 239, 309, 477, 591, 763, 783
BlpI GCTNAGC 1 cut(s) 75
BlsI GCNGC 6 cut(s) 240, 310, 478, 592, 764, 784
Bme1390I CCNGG 4 cut(s) 59, 64, 170, 804
Bme18I GGWCC 3 cut(s) 95, 198, 1194
BmgT120I GGNCC 5 cut(s) 95, 198, 717, 1134, 1194
BmiI GGNNCC 3 cut(s) 174, 412, 1120
BmrFI CCNGG 4 cut(s) 59, 64, 170, 804
BmsI GCATC 1 cut(s) 370
BplI GAGNNNNNCTC 2 cut(s) 58, 90
BpmI CTGGAG 2 cut(s) 85, 675
Bpu1102I GCTNAGC 1 cut(s) 75
BpuMI CCSGG 2 cut(s) 170, 804
BsaBI GATNNNNATC 1 cut(s) 731
BsaHI GRCGYC 1 cut(s) 1119
BsaJI CCNNGG 4 cut(s) 57, 517, 803, 1137
BsaWI WCCGGW 2 cut(s) 818, 1041
Bsc4I CCNNNNNNNGG 6 cut(s) 63, 195, 825, 920, 1105, 1203
Bse1I ACTGG 2 cut(s) 848, 914
Bse3DI GCAATG 2 cut(s) 98, 627
Bse8I GATNNNNATC 1 cut(s) 731
BseAI TCCGGA 1 cut(s) 1041
BseBI CCWGG 2 cut(s) 59, 64
BseDI CCNNGG 4 cut(s) 57, 517, 803, 1137
BseGI GGATG 1 cut(s) 799
BseJI GATNNNNATC 1 cut(s) 731
BseLI CCNNNNNNNGG 6 cut(s) 63, 195, 825, 920, 1105, 1203
BseMI GCAATG 2 cut(s) 98, 627
BseMII CTCAG 2 cut(s) 89, 921
BseNI ACTGG 2 cut(s) 848, 914
BseXI GCAGC 6 cut(s) 225, 295, 488, 577, 749, 794
BshFI GGCC 4 cut(s) 62, 516, 719, 1135
BshNI GGYRCC 1 cut(s) 1118
BsiSI CCGG 4 cut(s) 170, 803, 819, 1042
BslFI GGGAC 3 cut(s) 638, 670, 1201
BslI CCNNNNNNNGG 6 cut(s) 63, 195, 825, 920, 1105, 1203
BsmAI GTCTC 2 cut(s) 139, 225
BsmBI CGTCTC 1 cut(s) 139
BsmFI GGGAC 3 cut(s) 638, 670, 1201
BsmI GAATGC 1 cut(s) 1220
BsnI GGCC 4 cut(s) 62, 516, 719, 1135
Bsp13I TCCGGA 1 cut(s) 1041
Bsp1407I TGTACA 3 cut(s) 262, 374, 1171
Bsp143I GATC 3 cut(s) 172, 935, 1265
Bsp1720I GCTNAGC 1 cut(s) 75
BspACI CCGC 1 cut(s) 765
BspANI GGCC 4 cut(s) 62, 516, 719, 1135
BspCNI CTCAG 2 cut(s) 88, 922
BspEI TCCGGA 1 cut(s) 1041
BspHI TCATGA 1 cut(s) 105
BspLI GGNNCC 3 cut(s) 174, 412, 1120
BspMI ACCTGC 1 cut(s) 83
BspPI GGATC 3 cut(s) 167, 180, 1273
BspT107I GGYRCC 1 cut(s) 1118
BsrDI GCAATG 2 cut(s) 98, 627
BsrGI TGTACA 3 cut(s) 262, 374, 1171
BsrI ACTGG 2 cut(s) 848, 914
BssECI CCNNGG 4 cut(s) 57, 517, 803, 1137
BssMI GATC 3 cut(s) 172, 935, 1265
BssNI GRCGYC 1 cut(s) 1119
BssT1I CCWWGG 1 cut(s) 1137
Bst2UI CCWGG 2 cut(s) 59, 64
Bst4CI ACNGT 5 cut(s) 344, 962, 1048, 1069, 1249
Bst6I CTCTTC 1 cut(s) 707
BstACI GRCGYC 1 cut(s) 1119
BstAUI TGTACA 3 cut(s) 262, 374, 1171
BstC8I GCNNGC 2 cut(s) 313, 868
BstDEI CTNAG 4 cut(s) 75, 226, 930, 1295
BstEII GGTNACC 1 cut(s) 124
BstF5I GGATG 1 cut(s) 799
BstH2I RGCGCY 1 cut(s) 1122
BstHHI GCGC 1 cut(s) 1121
BstKTI GATC 3 cut(s) 175, 938, 1268
BstMAI GTCTC 2 cut(s) 139, 225
BstMBI GATC 3 cut(s) 172, 935, 1265
BstMWI GCNNNNNNNGC 3 cut(s) 279, 768, 844
BstNI CCWGG 2 cut(s) 59, 64
BstPI GGTNACC 1 cut(s) 124
BstSCI CCNGG 4 cut(s) 57, 62, 168, 802
BstSFI CTRYAG 4 cut(s) 160, 904, 1065, 1155
BstV1I GCAGC 6 cut(s) 225, 295, 488, 577, 749, 794
BstX2I RGATCY 1 cut(s) 172
BstXI CCANNNNNNTGG 1 cut(s) 620
BstYI RGATCY 1 cut(s) 172
BsuI GTATCC 3 cut(s) 379, 667, 963
BsuRI GGCC 4 cut(s) 62, 516, 719, 1135
BtsCI GGATG 1 cut(s) 799
BtsI GCAGTG 2 cut(s) 289, 322
BtsIMutI CAGTG 3 cut(s) 289, 322, 967
BveI ACCTGC 1 cut(s) 83
Cac8I GCNNGC 2 cut(s) 313, 868
CaiI CAGNNNCTG 1 cut(s) 311
CciI TCATGA 1 cut(s) 105
CfoI GCGC 1 cut(s) 1121
Cfr13I GGNCC 5 cut(s) 95, 198, 717, 1134, 1194
Csp6I GTAC 6 cut(s) 223, 263, 375, 416, 1165, 1172
CviAII CATG 6 cut(s) 106, 266, 372, 454, 484, 1191
CviQI GTAC 6 cut(s) 223, 263, 375, 416, 1165, 1172
DdeI CTNAG 4 cut(s) 75, 226, 930, 1295
DinI GGCGCC 1 cut(s) 1120
DpnI GATC 3 cut(s) 174, 937, 1267
DpnII GATC 3 cut(s) 172, 935, 1265
Eam1104I CTCTTC 1 cut(s) 707
EarI CTCTTC 1 cut(s) 707
Eco130I CCWWGG 1 cut(s) 1137
Eco32I GATATC 1 cut(s) 896
Eco47I GGWCC 3 cut(s) 95, 198, 1194
Eco57I CTGAAG 1 cut(s) 1012
Eco91I GGTNACC 1 cut(s) 124
EcoO109I RGGNCCY 2 cut(s) 198, 717
EcoO65I GGTNACC 1 cut(s) 124
EcoRII CCWGG 2 cut(s) 57, 62
EcoRV GATATC 1 cut(s) 896
EcoT14I CCWWGG 1 cut(s) 1137
EgeI GGCGCC 1 cut(s) 1120
EheI GGCGCC 1 cut(s) 1120
ErhI CCWWGG 1 cut(s) 1137
Esp3I CGTCTC 1 cut(s) 139
FaeI CATG 6 cut(s) 109, 269, 375, 457, 487, 1194
FalI AAGNNNNNCTT 3 cut(s) 209, 241, 1278
FaqI GGGAC 3 cut(s) 638, 670, 1201
FatI CATG 6 cut(s) 105, 265, 371, 453, 483, 1190
FauNDI CATATG 2 cut(s) 178, 379
Fnu4HI GCNGC 6 cut(s) 239, 309, 477, 591, 763, 783
FokI GGATG 1 cut(s) 806
Fsp4HI GCNGC 6 cut(s) 239, 309, 477, 591, 763, 783
FspBI CTAG 3 cut(s) 80, 582, 1300
GlaI GCGC 1 cut(s) 1120
GluI GCNGC 6 cut(s) 239, 309, 477, 591, 763, 783
GsuI CTGGAG 2 cut(s) 85, 675
HaeII RGCGCY 1 cut(s) 1122
HaeIII GGCC 4 cut(s) 62, 516, 719, 1135
HapII CCGG 4 cut(s) 170, 803, 819, 1042
HhaI GCGC 1 cut(s) 1121
Hin1I GRCGYC 1 cut(s) 1119
Hin1II CATG 6 cut(s) 109, 269, 375, 457, 487, 1194
Hin6I GCGC 1 cut(s) 1119
HinP1I GCGC 1 cut(s) 1119
HinfI GANTC 3 cut(s) 667, 808, 1021
HpaII CCGG 4 cut(s) 170, 803, 819, 1042
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 1 cut(s) 154
Hpy188III TCNNGA 5 cut(s) 106, 567, 671, 814, 1042
Hpy8I GTNNAC 1 cut(s) 6
HpyAV CCTTC 3 cut(s) 350, 1036, 1268
HpyCH4III ACNGT 5 cut(s) 344, 962, 1048, 1069, 1249
HpyCH4IV ACGT 1 cut(s) 677
HpyCH4V TGCA 8 cut(s) 92, 241, 476, 575, 782, 870, 965, 1076
HpyF10VI GCNNNNNNNGC 3 cut(s) 279, 768, 844
HpyF3I CTNAG 4 cut(s) 75, 226, 930, 1295
HpySE526I ACGT 1 cut(s) 677
Hsp92I GRCGYC 1 cut(s) 1119
Hsp92II CATG 6 cut(s) 109, 269, 375, 457, 487, 1194
HspAI GCGC 1 cut(s) 1119
KasI GGCGCC 1 cut(s) 1118
Kpn2I TCCGGA 1 cut(s) 1041
Kzo9I GATC 3 cut(s) 172, 935, 1265
LmnI GCTCC 2 cut(s) 410, 558
Lsp1109I GCAGC 6 cut(s) 225, 295, 488, 577, 749, 794
LweI GCATC 1 cut(s) 370
MaeI CTAG 3 cut(s) 80, 582, 1300
MaeII ACGT 1 cut(s) 677
MaeIII GTNAC 5 cut(s) 124, 247, 429, 739, 1243
MalI GATC 3 cut(s) 174, 937, 1267
MboI GATC 3 cut(s) 172, 935, 1265
MboII GAAGA 4 cut(s) 601, 724, 742, 869
MfeI CAATTG 1 cut(s) 618
MflI RGATCY 1 cut(s) 172
MluCI AATT 5 cut(s) 288, 596, 618, 1071, 1182
Mly113I GGCGCC 1 cut(s) 1119
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 8 cut(s) 527, 589, 699, 708, 730, 767, 884, 1091
MroI TCCGGA 1 cut(s) 1041
MroXI GAANNNNTTC 1 cut(s) 1025
MseI TTAA 3 cut(s) 552, 605, 680
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 4 cut(s) 170, 803, 819, 1042
MspR9I CCNGG 4 cut(s) 59, 64, 170, 804
MunI CAATTG 1 cut(s) 618
Mva1269I GAATGC 1 cut(s) 1220
MvaI CCWGG 2 cut(s) 59, 64
MwoI GCNNNNNNNGC 3 cut(s) 279, 768, 844
NarI GGCGCC 1 cut(s) 1119
NciI CCSGG 2 cut(s) 170, 804
NdeI CATATG 2 cut(s) 178, 379
NdeII GATC 3 cut(s) 172, 935, 1265
NlaIII CATG 6 cut(s) 109, 269, 375, 457, 487, 1194
NlaIV GGNNCC 3 cut(s) 174, 412, 1120
NmeAIII GCCGAG 1 cut(s) 498
NmuCI GTSAC 5 cut(s) 124, 247, 429, 739, 1243
PagI TCATGA 1 cut(s) 105
PctI GAATGC 1 cut(s) 1220
PdmI GAANNNNTTC 1 cut(s) 1025
PfeI GAWTC 3 cut(s) 667, 808, 1021
PflMI CCANNNNNTGG 3 cut(s) 920, 1105, 1203
PkrI GCNGC 6 cut(s) 240, 310, 478, 592, 764, 784
PluTI GGCGCC 1 cut(s) 1122
PpuMI RGGWCCY 1 cut(s) 198
PsiI TTATAA 1 cut(s) 1283
Psp5II RGGWCCY 1 cut(s) 198
Psp6I CCWGG 2 cut(s) 57, 62
PspEI GGTNACC 1 cut(s) 124
PspGI CCWGG 2 cut(s) 57, 62
PspN4I GGNNCC 3 cut(s) 174, 412, 1120
PspPI GGNCC 5 cut(s) 95, 198, 717, 1134, 1194
PspPPI RGGWCCY 1 cut(s) 198
PstNI CAGNNNCTG 1 cut(s) 311
PsuI RGATCY 1 cut(s) 172
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 6 cut(s) 224, 264, 376, 417, 1166, 1173
RsaNI GTAC 6 cut(s) 223, 263, 375, 416, 1165, 1172
SaqAI TTAA 3 cut(s) 552, 605, 680
SatI GCNGC 6 cut(s) 239, 309, 477, 591, 763, 783
Sau3AI GATC 3 cut(s) 172, 935, 1265
Sau96I GGNCC 5 cut(s) 95, 198, 717, 1134, 1194
ScrFI CCNGG 4 cut(s) 59, 64, 170, 804
SfaNI GCATC 1 cut(s) 370
SfcI CTRYAG 4 cut(s) 160, 904, 1065, 1155
SfoI GGCGCC 1 cut(s) 1120
SinI GGWCC 3 cut(s) 95, 198, 1194
Sse9I AATT 5 cut(s) 288, 596, 618, 1071, 1182
SsiI CCGC 1 cut(s) 765
SspDI GGCGCC 1 cut(s) 1118
SspMI CTAG 3 cut(s) 80, 582, 1300
StyD4I CCNGG 4 cut(s) 57, 62, 168, 802
StyI CCWWGG 1 cut(s) 1137
TaaI ACNGT 5 cut(s) 344, 962, 1048, 1069, 1249
TaiI ACGT 1 cut(s) 680
TaqII GACCGA 1 cut(s) 112
TasI AATT 5 cut(s) 288, 596, 618, 1071, 1182
TatI WGTACW 5 cut(s) 222, 262, 374, 1164, 1171
TfiI GAWTC 3 cut(s) 667, 808, 1021
Tru1I TTAA 3 cut(s) 552, 605, 680
Tru9I TTAA 3 cut(s) 552, 605, 680
TscAI CASTG 3 cut(s) 289, 322, 967
TseFI GTSAC 5 cut(s) 124, 247, 429, 739, 1243
TseI GCWGC 6 cut(s) 238, 308, 476, 590, 762, 782
Tsp45I GTSAC 5 cut(s) 124, 247, 429, 739, 1243
TspDTI ATGAA 4 cut(s) 360, 470, 725, 869
TspRI CASTG 3 cut(s) 289, 322, 967
Van91I CCANNNNNTGG 3 cut(s) 920, 1105, 1203
VpaK11BI GGWCC 3 cut(s) 95, 198, 1194
XmnI GAANNNNTTC 1 cut(s) 1025
XspI CTAG 3 cut(s) 80, 582, 1300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.