MD11G1041800.v1.1

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002116
Physical Location & Seq
Forward (+)
3567257 .. 3579194
11938 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1041800.v1.1.491

Sequence Viewer

Length: 1413 bp
ATGGGTGTTGGTGAAGCAGCAGAAGATCAGCAGTTACCGTTGCTCATAAGTTCTTCATCAGATGAACTCAACCAACCTCTCAAAAGAACTGGAACTCTACCGACTGCAGTAGCACATATAATAACAGGTGTGATAGGAGCAGGAGTATTATCCCTTGCATGGAGTGTGGCTCAGCTAGGGTGGATTGGAGGTCCATTGCTTATGATTGTATTTGCTTCAGTCACCATTCTTTCAACCAACCTCCTCTCCGACTGCTACCGGTACCCCGAATCCGATAGCCCGACGAGGGTCGGATCCTACTTAGAAGCTGTCAAGGTCTACCTAGGAGAGAAGAGTCAGACAATATGTGCAATATTTGTACAGGAGAGCCTGTATGGCACCGGAATTGCCTACATTATTACAGCTGCTGGCAGCGTCAAGAACGCTGTGGAGTTGTCCATTCAGAGGTCAAACTGCTACCACAAAGAAGGGCATGAAGCTTCATGTGAATATGGAACTAGTCTGTATATGCTGCTATTTGGACTTGTCCAGATTGTTATGTCACAAATACCAGATTTCCACAACATGGAATGGCTTTCAGTTATTGCAGCAATCATGTCCTTTACCTACTCTTTCATTGGACTTGGCCTCGGCTTTGCGAAAGTAATAGAAAATGGGAAGATTCAAGGAAGCCTAACAGGAGTTCCAACATCAAATGTTGCTGATAAATTATGGTTAACCTTCCAAGCACTTGGAGACATTGCTTTTGCCTACCCATACTCTATTATTGTTCTTGAAATTCAGGATACTTTGAAGTCCCCTCCACCCGAAAATCAGACAATGAAGAAGGCCTCAATGATTGCCATATTTGTCACAACCTTCTTCTACCTCTGCTGTGGATGCTTTGGATATGCCGCCTTTGGGGATGACACGCCAGGGAACCTCTTGACAGGATTTGGATTCTACGAACCCTACTGGCTCATTGATTTTGCCAATGCTTGCATTGTTCTTCATTTGGTGGGAGGATATCAGATCTTCAGTCAACCAGTATTTGCAGTTGCTGAGAGATGGTCTAGGAAGAAATACCCGAGCAGTGGATTTGTGAACAACTTTTACTCTATCAAAGTCCCATTGTTACCGAGATTTCAAGTGAATCCCTTCAGGCTTTGTTTCCGAACTGTTTACGTTGTGTCAACTACCGCGATTGCGATGATATTTCCTTACTTCAACCAGGTTTTGGGAGTTTTAGGGGCCTTGAACTTTTGGCCTTTGGCCATATATTTCCCTGTAGAAATGTACTTTGTACAGAAGAAAATTGGGGCTTGGACAAAAACATGGGTTGTTCTGAGGACATTTAGTATAGCTTGCTTTCTTGTGACAGTTGTGGGTGTGATTGGATCAGTTGAAGGACTTATAAGTGCCAAACTGAGCTAG

Protein Analysis

471

Amino Acids

51.56

Weight (kDa)

6.23

Isoelectric Point (pI)

30.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 30 - 465 5.2e-96 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1394
Acc65I GGTACC 1 cut(s) 261
AccB1I GGYRCC 2 cut(s) 261, 377
AccB7I CCANNNNNTGG 2 cut(s) 565, 1216
AccI GTMKAC 1 cut(s) 318
AccII CGCG 1 cut(s) 1181
AciI CCGC 2 cut(s) 894, 1179
AclWI GGATC 3 cut(s) 288, 301, 1384
AcoI YGGCCR 1 cut(s) 1251
AcsI RAATTY 1 cut(s) 777
AcuI CTGAAG 3 cut(s) 201, 1000, 1123
AfaI GTAC 4 cut(s) 263, 360, 1277, 1284
AfiI CCNNNNNNNGG 7 cut(s) 159, 286, 444, 565, 900, 1073, 1216
AgeI ACCGGT 1 cut(s) 258
AgsI TTSAA 8 cut(s) 234, 665, 776, 793, 1127, 1207, 1237, 1385
AhlI ACTAGT 1 cut(s) 497
AjnI CCWGG 2 cut(s) 913, 1209
AluBI AGCT 6 cut(s) 175, 308, 404, 479, 1343, 1410
AluI AGCT 6 cut(s) 175, 308, 404, 479, 1343, 1410
Alw26I GTCTC 1 cut(s) 729
AlwI GGATC 3 cut(s) 288, 301, 1384
AlwNI CAGNNNCTG 2 cut(s) 407, 1040
Ama87I CYCGRG 1 cut(s) 1066
AoxI GGCC 5 cut(s) 625, 828, 1230, 1244, 1251
ApeKI GCWGC 5 cut(s) 17, 404, 411, 511, 587
ApoI RAATTY 1 cut(s) 777
ArsI GACNNNNNNTTYG 2 cut(s) 728, 760
AsiGI ACCGGT 1 cut(s) 258
Asp700I GAANNNNTTC 1 cut(s) 1136
Asp718I GGTACC 1 cut(s) 261
AspA2I CCTAGG 1 cut(s) 322
AspS9I GGNCC 2 cut(s) 191, 1230
AsuHPI GGTGA 2 cut(s) 23, 214
AvaI CYCGRG 1 cut(s) 1066
AvaII GGWCC 1 cut(s) 191
AvrII CCTAGG 1 cut(s) 322
BalI TGGCCA 1 cut(s) 1253
BamHI GGATCC 1 cut(s) 293
BanI GGYRCC 2 cut(s) 261, 377
BbvI GCAGC 5 cut(s) 29, 391, 423, 498, 599
BccI CCATC 1 cut(s) 1041
BciT130I CCWGG 2 cut(s) 915, 1211
BciVI GTATCC 1 cut(s) 778
BcoDI GTCTC 1 cut(s) 729
BcuI ACTAGT 1 cut(s) 497
BfaI CTAG 5 cut(s) 176, 323, 498, 1053, 1411
BfmI CTRYAG 2 cut(s) 105, 1266
BfuI GTATCC 1 cut(s) 778
BglI GCCNNNNNGGC 1 cut(s) 375
BglII AGATCT 1 cut(s) 1011
BisI GCNGC 6 cut(s) 18, 405, 412, 512, 588, 894
BlnI CCTAGG 1 cut(s) 322
BlpI GCTNAGC 1 cut(s) 171
BlsI GCNGC 6 cut(s) 19, 406, 413, 513, 589, 895
Bme1390I CCNGG 2 cut(s) 915, 1211
Bme18I GGWCC 1 cut(s) 191
BmeT110I CYCGRG 1 cut(s) 1066
BmgT120I GGNCC 2 cut(s) 191, 1230
BmiI GGNNCC 5 cut(s) 263, 295, 379, 920, 1231
BmrFI CCNGG 2 cut(s) 915, 1211
BmsI GCATC 1 cut(s) 869
BoxI GACNNNNGTC 1 cut(s) 287
BplI GAGNNNNNCTC 2 cut(s) 154, 186
Bpu1102I GCTNAGC 1 cut(s) 171
BsaJI CCNNGG 3 cut(s) 322, 628, 914
BsaWI WCCGGW 2 cut(s) 258, 380
BsaXI ACNNNNNCTCC 6 cut(s) 129, 159, 230, 260, 356, 386
Bsc4I CCNNNNNNNGG 7 cut(s) 159, 286, 444, 565, 900, 1073, 1216
Bse118I RCCGGY 1 cut(s) 258
Bse1I ACTGG 3 cut(s) 94, 959, 1025
Bse3DI GCAATG 2 cut(s) 194, 738
BseBI CCWGG 2 cut(s) 915, 1211
BseDI CCNNGG 3 cut(s) 322, 628, 914
BseGI GGATG 2 cut(s) 884, 910
BseLI CCNNNNNNNGG 7 cut(s) 159, 286, 444, 565, 900, 1073, 1216
BseMI GCAATG 2 cut(s) 194, 738
BseMII CTCAG 4 cut(s) 185, 1032, 1316, 1397
BseNI ACTGG 3 cut(s) 94, 959, 1025
BseRI GAGGAG 1 cut(s) 233
BseXI GCAGC 5 cut(s) 29, 391, 423, 498, 599
Bsh1236I CGCG 1 cut(s) 1181
BshFI GGCC 5 cut(s) 627, 830, 1232, 1246, 1253
BshNI GGYRCC 2 cut(s) 261, 377
BshTI ACCGGT 1 cut(s) 258
BsiHKCI CYCGRG 1 cut(s) 1066
BsiSI CCGG 2 cut(s) 259, 381
BslFI GGGAC 2 cut(s) 781, 1091
BslI CCNNNNNNNGG 7 cut(s) 159, 286, 444, 565, 900, 1073, 1216
BsmAI GTCTC 1 cut(s) 729
BsmFI GGGAC 2 cut(s) 781, 1091
BsnI GGCC 5 cut(s) 627, 830, 1232, 1246, 1253
BsoBI CYCGRG 1 cut(s) 1066
Bsp1407I TGTACA 2 cut(s) 358, 1282
Bsp143I GATC 4 cut(s) 25, 293, 1011, 1376
Bsp1720I GCTNAGC 1 cut(s) 171
BspACI CCGC 2 cut(s) 894, 1179
BspANI GGCC 5 cut(s) 627, 830, 1232, 1246, 1253
BspCNI CTCAG 4 cut(s) 184, 1033, 1317, 1398
BspFNI CGCG 1 cut(s) 1181
BspLI GGNNCC 5 cut(s) 263, 295, 379, 920, 1231
BspMAI CTGCAG 1 cut(s) 109
BspPI GGATC 3 cut(s) 288, 301, 1384
BspT107I GGYRCC 2 cut(s) 261, 377
BsrDI GCAATG 2 cut(s) 194, 738
BsrFI RCCGGY 1 cut(s) 258
BsrGI TGTACA 2 cut(s) 358, 1282
BsrI ACTGG 3 cut(s) 94, 959, 1025
BssAI RCCGGY 1 cut(s) 258
BssECI CCNNGG 3 cut(s) 322, 628, 914
BssMI GATC 4 cut(s) 25, 293, 1011, 1376
BssT1I CCWWGG 1 cut(s) 322
Bst2UI CCWGG 2 cut(s) 915, 1211
Bst4CI ACNGT 3 cut(s) 39, 1159, 1360
Bst6I CTCTTC 1 cut(s) 326
BstAUI TGTACA 2 cut(s) 358, 1282
BstC8I GCNNGC 3 cut(s) 409, 979, 1345
BstDEI CTNAG 5 cut(s) 171, 301, 1041, 1325, 1406
BstF5I GGATG 2 cut(s) 884, 910
BstFNI CGCG 1 cut(s) 1181
BstKTI GATC 4 cut(s) 28, 296, 1014, 1379
BstMAI GTCTC 1 cut(s) 729
BstMBI GATC 4 cut(s) 25, 293, 1011, 1376
BstMWI GCNNNNNNNGC 2 cut(s) 375, 879
BstNI CCWGG 2 cut(s) 915, 1211
BstPAI GACNNNNGTC 1 cut(s) 287
BstSCI CCNGG 2 cut(s) 913, 1209
BstSFI CTRYAG 2 cut(s) 105, 1266
BstUI CGCG 1 cut(s) 1181
BstV1I GCAGC 5 cut(s) 29, 391, 423, 498, 599
BstX2I RGATCY 2 cut(s) 293, 1011
BstXI CCANNNNNNTGG 1 cut(s) 731
BstYI RGATCY 2 cut(s) 293, 1011
BsuI GTATCC 1 cut(s) 778
BsuRI GGCC 5 cut(s) 627, 830, 1232, 1246, 1253
BtgZI GCGATG 1 cut(s) 1202
BtsCI GGATG 2 cut(s) 884, 910
BtsI GCAGTG 1 cut(s) 1078
BtsIMutI CAGTG 1 cut(s) 1078
Cac8I GCNNGC 3 cut(s) 409, 979, 1345
CaiI CAGNNNCTG 2 cut(s) 407, 1040
Cfr10I RCCGGY 1 cut(s) 258
Cfr13I GGNCC 2 cut(s) 191, 1230
CseI GACGC 1 cut(s) 403
CsiI ACCWGGT 1 cut(s) 1209
Csp6I GTAC 4 cut(s) 262, 359, 1276, 1283
CspAI ACCGGT 1 cut(s) 258
CviAII CATG 6 cut(s) 159, 473, 483, 565, 595, 1314
CviQI GTAC 4 cut(s) 262, 359, 1276, 1283
DdeI CTNAG 5 cut(s) 171, 301, 1041, 1325, 1406
DpnI GATC 4 cut(s) 27, 295, 1013, 1378
DpnII GATC 4 cut(s) 25, 293, 1011, 1376
EaeI YGGCCR 1 cut(s) 1251
Eam1104I CTCTTC 1 cut(s) 326
EarI CTCTTC 1 cut(s) 326
Eco130I CCWWGG 1 cut(s) 322
Eco147I AGGCCT 1 cut(s) 830
Eco32I GATATC 1 cut(s) 1007
Eco47I GGWCC 1 cut(s) 191
Eco57I CTGAAG 3 cut(s) 201, 1000, 1123
Eco88I CYCGRG 1 cut(s) 1066
EcoO109I RGGNCCY 1 cut(s) 1230
EcoRII CCWGG 2 cut(s) 913, 1209
EcoRV GATATC 1 cut(s) 1007
EcoT14I CCWWGG 1 cut(s) 322
ErhI CCWWGG 1 cut(s) 322
FaeI CATG 6 cut(s) 162, 476, 486, 568, 598, 1317
FaqI GGGAC 2 cut(s) 781, 1091
FatI CATG 6 cut(s) 158, 472, 482, 564, 594, 1313
FblI GTMKAC 1 cut(s) 318
Fnu4HI GCNGC 6 cut(s) 18, 405, 412, 512, 588, 894
FokI GGATG 2 cut(s) 891, 917
Fsp4HI GCNGC 6 cut(s) 18, 405, 412, 512, 588, 894
FspBI CTAG 5 cut(s) 176, 323, 498, 1053, 1411
GluI GCNGC 6 cut(s) 18, 405, 412, 512, 588, 894
HaeIII GGCC 5 cut(s) 627, 830, 1232, 1246, 1253
HapII CCGG 2 cut(s) 259, 381
HgaI GACGC 1 cut(s) 403
Hin1II CATG 6 cut(s) 162, 476, 486, 568, 598, 1317
HincII GTYRAC 3 cut(s) 717, 1022, 1173
HindII GTYRAC 3 cut(s) 717, 1022, 1173
HindIII AAGCTT 1 cut(s) 477
HinfI GANTC 5 cut(s) 269, 334, 661, 939, 1132
HpaI GTTAAC 1 cut(s) 717
HpaII CCGG 2 cut(s) 259, 381
HphI GGTGA 2 cut(s) 23, 214
Hpy166II GTNNAC 6 cut(s) 319, 717, 1022, 1084, 1162, 1173
Hpy188III TCNNGA 5 cut(s) 418, 529, 773, 782, 925
Hpy8I GTNNAC 6 cut(s) 319, 717, 1022, 1084, 1162, 1173
Hpy99I CGWCG 1 cut(s) 286
HpyAV CCTTC 6 cut(s) 461, 730, 820, 868, 1147, 1379
HpyCH4III ACNGT 3 cut(s) 39, 1159, 1360
HpyCH4IV ACGT 1 cut(s) 1164
HpyCH4V TGCA 6 cut(s) 107, 158, 350, 587, 981, 1034
HpyF10VI GCNNNNNNNGC 2 cut(s) 375, 879
HpyF3I CTNAG 5 cut(s) 171, 301, 1041, 1325, 1406
HpySE526I ACGT 1 cut(s) 1164
Hsp92II CATG 6 cut(s) 162, 476, 486, 568, 598, 1317
KpnI GGTACC 1 cut(s) 265
KspAI GTTAAC 1 cut(s) 717
Kzo9I GATC 4 cut(s) 25, 293, 1011, 1376
LmnI GCTCC 1 cut(s) 137
Lsp1109I GCAGC 5 cut(s) 29, 391, 423, 498, 599
LweI GCATC 1 cut(s) 869
MabI ACCWGGT 1 cut(s) 1209
MaeI CTAG 5 cut(s) 176, 323, 498, 1053, 1411
MaeII ACGT 1 cut(s) 1164
MaeIII GTNAC 6 cut(s) 33, 220, 540, 850, 1113, 1354
MalI GATC 4 cut(s) 27, 295, 1013, 1378
MboI GATC 4 cut(s) 25, 293, 1011, 1376
MflI RGATCY 2 cut(s) 293, 1011
MlsI TGGCCA 1 cut(s) 1253
MluCI AATT 4 cut(s) 384, 707, 777, 1293
MluNI TGGCCA 1 cut(s) 1253
MlyI GAGTC 1 cut(s) 343
MmeI TCCRAC 3 cut(s) 271, 273, 710
Mox20I TGGCCA 1 cut(s) 1253
MroXI GAANNNNTTC 1 cut(s) 1136
MscI TGGCCA 1 cut(s) 1253
MseI TTAA 1 cut(s) 716
Msp20I TGGCCA 1 cut(s) 1253
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 2 cut(s) 259, 381
MspR9I CCNGG 2 cut(s) 915, 1211
MvaI CCWGG 2 cut(s) 915, 1211
MvnI CGCG 1 cut(s) 1181
MwoI GCNNNNNNNGC 2 cut(s) 375, 879
NdeII GATC 4 cut(s) 25, 293, 1011, 1376
NlaIII CATG 6 cut(s) 162, 476, 486, 568, 598, 1317
NlaIV GGNNCC 5 cut(s) 263, 295, 379, 920, 1231
NmeAIII GCCGAG 1 cut(s) 609
NmuCI GTSAC 4 cut(s) 220, 540, 850, 1354
PceI AGGCCT 1 cut(s) 830
PdmI GAANNNNTTC 1 cut(s) 1136
PfeI GAWTC 4 cut(s) 269, 661, 939, 1132
PflMI CCANNNNNTGG 2 cut(s) 565, 1216
PinAI ACCGGT 1 cut(s) 258
PkrI GCNGC 6 cut(s) 19, 406, 413, 513, 589, 895
PleI GAGTC 1 cut(s) 342
PpsI GAGTC 1 cut(s) 342
PshAI GACNNNNGTC 1 cut(s) 287
PsiI TTATAA 1 cut(s) 1394
Psp6I CCWGG 2 cut(s) 913, 1209
PspGI CCWGG 2 cut(s) 913, 1209
PspN4I GGNNCC 5 cut(s) 263, 295, 379, 920, 1231
PspPI GGNCC 2 cut(s) 191, 1230
PsrI GAACNNNNNNTAC 2 cut(s) 1076, 1108
PstI CTGCAG 1 cut(s) 109
PstNI CAGNNNCTG 2 cut(s) 407, 1040
PsuI RGATCY 2 cut(s) 293, 1011
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 4 cut(s) 263, 360, 1277, 1284
RsaNI GTAC 4 cut(s) 262, 359, 1276, 1283
SaqAI TTAA 1 cut(s) 716
SatI GCNGC 6 cut(s) 18, 405, 412, 512, 588, 894
Sau3AI GATC 4 cut(s) 25, 293, 1011, 1376
Sau96I GGNCC 2 cut(s) 191, 1230
SchI GAGTC 1 cut(s) 343
ScrFI CCNGG 2 cut(s) 915, 1211
SexAI ACCWGGT 1 cut(s) 1209
SfaNI GCATC 1 cut(s) 869
SfcI CTRYAG 2 cut(s) 105, 1266
SinI GGWCC 1 cut(s) 191
SpeI ACTAGT 1 cut(s) 497
Sse9I AATT 4 cut(s) 384, 707, 777, 1293
SseBI AGGCCT 1 cut(s) 830
SsiI CCGC 2 cut(s) 894, 1179
SspI AATATT 1 cut(s) 354
SspMI CTAG 5 cut(s) 176, 323, 498, 1053, 1411
StuI AGGCCT 1 cut(s) 830
StyD4I CCNGG 2 cut(s) 913, 1209
StyI CCWWGG 1 cut(s) 322
TaaI ACNGT 3 cut(s) 39, 1159, 1360
TaiI ACGT 1 cut(s) 1167
TasI AATT 4 cut(s) 384, 707, 777, 1293
TatI WGTACW 3 cut(s) 358, 1275, 1282
TauI GCSGC 1 cut(s) 896
TfiI GAWTC 4 cut(s) 269, 661, 939, 1132
Tru1I TTAA 1 cut(s) 716
Tru9I TTAA 1 cut(s) 716
TscAI CASTG 1 cut(s) 1078
TseFI GTSAC 4 cut(s) 220, 540, 850, 1354
TseI GCWGC 5 cut(s) 17, 404, 411, 511, 587
Tsp45I GTSAC 4 cut(s) 220, 540, 850, 1354
TspDTI ATGAA 7 cut(s) 45, 78, 471, 489, 604, 836, 980
TspRI CASTG 1 cut(s) 1078
Van91I CCANNNNNTGG 2 cut(s) 565, 1216
VpaK11BI GGWCC 1 cut(s) 191
XapI RAATTY 1 cut(s) 777
XmaJI CCTAGG 1 cut(s) 322
XmiI GTMKAC 1 cut(s) 318
XmnI GAANNNNTTC 1 cut(s) 1136
XspI CTAG 5 cut(s) 176, 323, 498, 1053, 1411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.