Rh5CG537900

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0002116
Physical Location & Seq
Reverse (-)
75230338 .. 75235304
4967 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG537900.1

Sequence Viewer

Length: 1401 bp
ATGGGTGGGGAAGAAGCAGAACATGATCAGCAGTCACCATTGCTCCTTGGCTTTCCTTCAACAATGGAACCCAGTGAATCTATCAAAAGAACTGGGACCATGTGGACGGCAATAGCACATATAATAACAGGAGTAATAGGGGCAGGGGTGTTATCCCTGGCCTGGAGTGTGGCTCAACTAGGGTGGATTGCCGGTCCATTGCTCATGATAGTCTTTGCTTCGGTCACCATCGTCTCCAACAACATTCTCTCTGACTGCTACAGATACCCGGATCCATATGCCCCGACCCGAAACAGGTCCTATTTGGAAGCTGTGAAGCTGTACTTAGGAGACAAGCTGCAAACAGTGACTGCCGTATTTGTACATGAGAGCTTGTATGGCAGTGGAATTGCCTATACCATTACTGCTGCTGGCAGTGTCAAAGCGATTCTGAAGTCAAACTGTTATCACAAGGAAGGGCATCAGGCTTCATGTACATATGGGGATACTCTGTATATGCTGATATTTGGAGCCGTACAGATTGTTGTGTCACAAATACCAGATTTCCATAACATGAAATGGCTTTCGGTTATTGCAGCAATCATGTCCTTCGCCTACTCTTTTATTGGACTTGGCCTCGGCTTTGCCAAAGTAATAGAAAATGGGAGTGTTAAAGGGAGCTTGTCAGGAGTTTCAACCTCTAGTTTTGCTGCTAAATTATGGTTAATCTTCCAAGCAATTGGGGACATTGCTTTTGCCTATCCATACTCTATTATTCTTTTTGAGATTCAGGATACGTTAAAGTCCCCTCCAGCAGAAAACTACACGATGAAGAGGGCCTCTGTGATTGCCATCATTGTCACAACTTTTTTCTACCTCTGCTGCGGTTGCTTTGGATATGCAGCCTTTGGGGATGACACGCCGGGGAATCTCTTGACTGGATTTGGGTTTTACGAGCCTTACTGGCTCATTGATTTTGCTAATGCTTGCATAGTTCTTCATTTGGTGGGAGGATATCAGGTCTATAGTCAGCCAGTATTTGGAGTTGCTGAGAGATGGTTTAGCAAGAAGTATCCAAACAGTGCATTTGTGAACAACTTTTACAGCTTCAAAATCCCAATGTTTGCGAGATTTCAAGTGAATCCCTTCAGGCTATGTTTCCGGACTGTTTATGTCATTTCAACTACAGTAATTGCAATCCTCTTTCCTTATTTCAACCAAGTTTTGGGAGTGTTAGGCGCCCTGAACTTTTGGCCCTTGGTTATATTTTTTCCTGTAGAGATGTACTTTGTACAAAAGAAAATTGGGACATGGACCAAGAAATGGATAGTATTACAGGCATTCAGTTTTGTTTGCTTTCTTGTGACAGTTATGGGTGTCATTGGATCAGTTGAAGGACTTATAAGTGCCAAACTTAGCTAG

Protein Analysis

466

Amino Acids

51.46

Weight (kDa)

8.24

Isoelectric Point (pI)

31.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 30 - 461 1.4e-100 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1382
AccB1I GGYRCC 1 cut(s) 1217
AccB7I CCANNNNNTGG 3 cut(s) 1019, 1204, 1302
AccIII TCCGGA 1 cut(s) 1140
AciI CCGC 1 cut(s) 864
AclWI GGATC 3 cut(s) 266, 279, 1372
AcuI CTGAAG 2 cut(s) 452, 1111
AcyI GRCGYC 1 cut(s) 1218
AfaI GTAC 6 cut(s) 323, 363, 475, 516, 1265, 1272
AfiI CCNNNNNNNGG 5 cut(s) 162, 294, 1019, 1204, 1302
AgsI TTSAA 7 cut(s) 60, 675, 1090, 1115, 1161, 1195, 1373
AjnI CCWGG 2 cut(s) 156, 161
AluBI AGCT 7 cut(s) 311, 319, 337, 372, 660, 1086, 1398
AluI AGCT 7 cut(s) 311, 319, 337, 372, 660, 1086, 1398
Alw26I GTCTC 2 cut(s) 238, 324
AlwI GGATC 3 cut(s) 266, 279, 1372
AlwNI CAGNNNCTG 1 cut(s) 350
Aor13HI TCCGGA 1 cut(s) 1140
AoxI GGCC 4 cut(s) 159, 613, 816, 1232
ApeKI GCWGC 6 cut(s) 337, 407, 575, 689, 861, 881
ArsI GACNNNNNNTTYG 2 cut(s) 716, 748
Asp700I GAANNNNTTC 1 cut(s) 1124
AspLEI GCGC 1 cut(s) 1220
AspS9I GGNCC 6 cut(s) 96, 194, 297, 816, 1233, 1293
AsuC2I CCSGG 2 cut(s) 269, 903
AsuHPI GGTGA 2 cut(s) 27, 217
AvaII GGWCC 4 cut(s) 96, 194, 297, 1293
BamHI GGATCC 1 cut(s) 271
BanI GGYRCC 1 cut(s) 1217
BbvI GCAGC 6 cut(s) 324, 394, 587, 676, 848, 893
BccI CCATC 3 cut(s) 236, 839, 1029
BceAI ACGGC 3 cut(s) 123, 338, 497
BciT130I CCWGG 2 cut(s) 158, 163
BciVI GTATCC 3 cut(s) 478, 766, 1062
BclI TGATCA 1 cut(s) 25
BcnI CCSGG 2 cut(s) 269, 903
BcoDI GTCTC 2 cut(s) 238, 324
BfaI CTAG 3 cut(s) 179, 681, 1399
BfmI CTRYAG 4 cut(s) 259, 1003, 1164, 1254
BfoI RGCGCY 1 cut(s) 1221
BfuI GTATCC 3 cut(s) 478, 766, 1062
BglI GCCNNNNNGGC 1 cut(s) 943
BisI GCNGC 6 cut(s) 338, 408, 576, 690, 862, 882
BlsI GCNGC 6 cut(s) 339, 409, 577, 691, 863, 883
Bme1390I CCNGG 4 cut(s) 158, 163, 269, 903
Bme18I GGWCC 4 cut(s) 96, 194, 297, 1293
BmgT120I GGNCC 6 cut(s) 96, 194, 297, 816, 1233, 1293
BmiI GGNNCC 5 cut(s) 69, 97, 273, 511, 1219
BmrFI CCNGG 4 cut(s) 158, 163, 269, 903
BmrI ACTGGG 2 cut(s) 66, 102
BmsI GCATC 1 cut(s) 469
BmuI ACTGGG 2 cut(s) 66, 102
BplI GAGNNNNNCTC 2 cut(s) 157, 189
BpmI CTGGAG 2 cut(s) 184, 774
BpuMI CCSGG 2 cut(s) 269, 903
BsaBI GATNNNNATC 1 cut(s) 830
BsaHI GRCGYC 1 cut(s) 1218
BsaJI CCNNGG 5 cut(s) 46, 156, 616, 902, 1236
BsaWI WCCGGW 1 cut(s) 1140
BsaXI ACNNNNNCTCC 2 cut(s) 27, 57
Bsc4I CCNNNNNNNGG 5 cut(s) 162, 294, 1019, 1204, 1302
Bse118I RCCGGY 1 cut(s) 191
Bse1I ACTGG 5 cut(s) 72, 97, 922, 947, 1013
Bse3DI GCAATG 3 cut(s) 38, 197, 726
Bse8I GATNNNNATC 1 cut(s) 830
BseAI TCCGGA 1 cut(s) 1140
BseBI CCWGG 2 cut(s) 158, 163
BseDI CCNNGG 5 cut(s) 46, 156, 616, 902, 1236
BseGI GGATG 1 cut(s) 898
BseJI GATNNNNATC 1 cut(s) 830
BseLI CCNNNNNNNGG 5 cut(s) 162, 294, 1019, 1204, 1302
BseMI GCAATG 3 cut(s) 38, 197, 726
BseMII CTCAG 1 cut(s) 1020
BseNI ACTGG 5 cut(s) 72, 97, 922, 947, 1013
BseXI GCAGC 6 cut(s) 324, 394, 587, 676, 848, 893
BshFI GGCC 4 cut(s) 161, 615, 818, 1234
BshNI GGYRCC 1 cut(s) 1217
BsiSI CCGG 4 cut(s) 192, 269, 902, 1141
BslFI GGGAC 4 cut(s) 109, 737, 769, 1300
BslI CCNNNNNNNGG 5 cut(s) 162, 294, 1019, 1204, 1302
BsmAI GTCTC 2 cut(s) 238, 324
BsmBI CGTCTC 1 cut(s) 238
BsmFI GGGAC 4 cut(s) 109, 737, 769, 1300
BsmI GAATGC 1 cut(s) 1319
BsnI GGCC 4 cut(s) 161, 615, 818, 1234
Bsp13I TCCGGA 1 cut(s) 1140
Bsp1407I TGTACA 3 cut(s) 361, 473, 1270
Bsp143I GATC 3 cut(s) 25, 271, 1364
BspACI CCGC 1 cut(s) 864
BspANI GGCC 4 cut(s) 161, 615, 818, 1234
BspCNI CTCAG 1 cut(s) 1021
BspEI TCCGGA 1 cut(s) 1140
BspHI TCATGA 1 cut(s) 204
BspLI GGNNCC 5 cut(s) 69, 97, 273, 511, 1219
BspPI GGATC 3 cut(s) 266, 279, 1372
BspT107I GGYRCC 1 cut(s) 1217
BsrDI GCAATG 3 cut(s) 38, 197, 726
BsrFI RCCGGY 1 cut(s) 191
BsrGI TGTACA 3 cut(s) 361, 473, 1270
BsrI ACTGG 5 cut(s) 72, 97, 922, 947, 1013
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 5 cut(s) 46, 156, 616, 902, 1236
BssMI GATC 3 cut(s) 25, 271, 1364
BssNI GRCGYC 1 cut(s) 1218
BssT1I CCWWGG 2 cut(s) 46, 1236
Bst2UI CCWGG 2 cut(s) 158, 163
Bst4CI ACNGT 6 cut(s) 346, 443, 1061, 1147, 1168, 1348
Bst6I CTCTTC 1 cut(s) 806
BstACI GRCGYC 1 cut(s) 1218
BstAUI TGTACA 3 cut(s) 361, 473, 1270
BstC8I GCNNGC 2 cut(s) 412, 967
BstDEI CTNAG 3 cut(s) 325, 1029, 1394
BstEII GGTNACC 1 cut(s) 223
BstF5I GGATG 1 cut(s) 898
BstH2I RGCGCY 1 cut(s) 1221
BstHHI GCGC 1 cut(s) 1220
BstKTI GATC 3 cut(s) 28, 274, 1367
BstMAI GTCTC 2 cut(s) 238, 324
BstMBI GATC 3 cut(s) 25, 271, 1364
BstMWI GCNNNNNNNGC 3 cut(s) 378, 867, 943
BstNI CCWGG 2 cut(s) 158, 163
BstPI GGTNACC 1 cut(s) 223
BstSCI CCNGG 4 cut(s) 156, 161, 267, 901
BstSFI CTRYAG 4 cut(s) 259, 1003, 1164, 1254
BstV1I GCAGC 6 cut(s) 324, 394, 587, 676, 848, 893
BstX2I RGATCY 1 cut(s) 271
BstXI CCANNNNNNTGG 1 cut(s) 719
BstYI RGATCY 1 cut(s) 271
BsuI GTATCC 3 cut(s) 478, 766, 1062
BsuRI GGCC 4 cut(s) 161, 615, 818, 1234
BtsCI GGATG 1 cut(s) 898
BtsI GCAGTG 2 cut(s) 388, 421
BtsIMutI CAGTG 5 cut(s) 79, 351, 388, 421, 1066
Cac8I GCNNGC 2 cut(s) 412, 967
CaiI CAGNNNCTG 1 cut(s) 350
CciI TCATGA 1 cut(s) 204
CfoI GCGC 1 cut(s) 1220
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 6 cut(s) 96, 194, 297, 816, 1233, 1293
Csp6I GTAC 6 cut(s) 322, 362, 474, 515, 1264, 1271
CspCI CAANNNNNGTGG 2 cut(s) 164, 199
CviAII CATG 8 cut(s) 23, 100, 205, 365, 471, 553, 583, 1290
CviQI GTAC 6 cut(s) 322, 362, 474, 515, 1264, 1271
DdeI CTNAG 3 cut(s) 325, 1029, 1394
DinI GGCGCC 1 cut(s) 1219
DpnI GATC 3 cut(s) 27, 273, 1366
DpnII GATC 3 cut(s) 25, 271, 1364
Eam1104I CTCTTC 1 cut(s) 806
EarI CTCTTC 1 cut(s) 806
Eco130I CCWWGG 2 cut(s) 46, 1236
Eco32I GATATC 1 cut(s) 995
Eco47I GGWCC 4 cut(s) 96, 194, 297, 1293
Eco57I CTGAAG 2 cut(s) 452, 1111
Eco91I GGTNACC 1 cut(s) 223
EcoO109I RGGNCCY 2 cut(s) 297, 816
EcoO65I GGTNACC 1 cut(s) 223
EcoRII CCWGG 2 cut(s) 156, 161
EcoRV GATATC 1 cut(s) 995
EcoT14I CCWWGG 2 cut(s) 46, 1236
EgeI GGCGCC 1 cut(s) 1219
EheI GGCGCC 1 cut(s) 1219
ErhI CCWWGG 2 cut(s) 46, 1236
Esp3I CGTCTC 1 cut(s) 238
FaeI CATG 8 cut(s) 26, 103, 208, 368, 474, 556, 586, 1293
FalI AAGNNNNNCTT 2 cut(s) 308, 340
FaqI GGGAC 4 cut(s) 109, 737, 769, 1300
FatI CATG 8 cut(s) 22, 99, 204, 364, 470, 552, 582, 1289
FauNDI CATATG 2 cut(s) 277, 478
FbaI TGATCA 1 cut(s) 25
Fnu4HI GCNGC 6 cut(s) 338, 408, 576, 690, 862, 882
FokI GGATG 1 cut(s) 905
Fsp4HI GCNGC 6 cut(s) 338, 408, 576, 690, 862, 882
FspBI CTAG 3 cut(s) 179, 681, 1399
GlaI GCGC 1 cut(s) 1219
GluI GCNGC 6 cut(s) 338, 408, 576, 690, 862, 882
GsuI CTGGAG 2 cut(s) 184, 774
HaeII RGCGCY 1 cut(s) 1221
HaeIII GGCC 4 cut(s) 161, 615, 818, 1234
HapII CCGG 4 cut(s) 192, 269, 902, 1141
HhaI GCGC 1 cut(s) 1220
Hin1I GRCGYC 1 cut(s) 1218
Hin1II CATG 8 cut(s) 26, 103, 208, 368, 474, 556, 586, 1293
Hin6I GCGC 1 cut(s) 1218
HinP1I GCGC 1 cut(s) 1218
HinfI GANTC 5 cut(s) 77, 427, 766, 907, 1120
HpaII CCGG 4 cut(s) 192, 269, 902, 1141
HphI GGTGA 2 cut(s) 27, 217
Hpy166II GTNNAC 2 cut(s) 105, 1072
Hpy188I TCNGA 2 cut(s) 253, 432
Hpy188III TCNNGA 5 cut(s) 205, 666, 770, 913, 1141
Hpy8I GTNNAC 2 cut(s) 105, 1072
HpyAV CCTTC 5 cut(s) 66, 449, 598, 1135, 1367
HpyCH4III ACNGT 6 cut(s) 346, 443, 1061, 1147, 1168, 1348
HpyCH4IV ACGT 1 cut(s) 776
HpyCH4V TGCA 6 cut(s) 340, 575, 881, 969, 1064, 1175
HpyF10VI GCNNNNNNNGC 3 cut(s) 378, 867, 943
HpyF3I CTNAG 3 cut(s) 325, 1029, 1394
HpySE526I ACGT 1 cut(s) 776
Hsp92I GRCGYC 1 cut(s) 1218
Hsp92II CATG 8 cut(s) 26, 103, 208, 368, 474, 556, 586, 1293
HspAI GCGC 1 cut(s) 1218
KasI GGCGCC 1 cut(s) 1217
Kpn2I TCCGGA 1 cut(s) 1140
Ksp22I TGATCA 1 cut(s) 25
Kzo9I GATC 3 cut(s) 25, 271, 1364
LmnI GCTCC 3 cut(s) 48, 509, 657
Lsp1109I GCAGC 6 cut(s) 324, 394, 587, 676, 848, 893
LweI GCATC 1 cut(s) 469
MaeI CTAG 3 cut(s) 179, 681, 1399
MaeII ACGT 1 cut(s) 776
MaeIII GTNAC 6 cut(s) 33, 223, 346, 528, 838, 1342
MalI GATC 3 cut(s) 27, 273, 1366
MboI GATC 3 cut(s) 25, 271, 1364
MboII GAAGA 4 cut(s) 23, 700, 823, 968
MfeI CAATTG 1 cut(s) 717
MflI RGATCY 1 cut(s) 271
MluCI AATT 5 cut(s) 387, 695, 717, 1170, 1281
Mly113I GGCGCC 1 cut(s) 1218
MmeI TCCRAC 1 cut(s) 261
MnlI CCTC 8 cut(s) 626, 688, 798, 807, 829, 866, 983, 1190
MroI TCCGGA 1 cut(s) 1140
MroXI GAANNNNTTC 1 cut(s) 1124
MseI TTAA 3 cut(s) 651, 704, 779
MspI CCGG 4 cut(s) 192, 269, 902, 1141
MspR9I CCNGG 4 cut(s) 158, 163, 269, 903
MunI CAATTG 1 cut(s) 717
Mva1269I GAATGC 1 cut(s) 1319
MvaI CCWGG 2 cut(s) 158, 163
MwoI GCNNNNNNNGC 3 cut(s) 378, 867, 943
NarI GGCGCC 1 cut(s) 1218
NciI CCSGG 2 cut(s) 269, 903
NdeI CATATG 2 cut(s) 277, 478
NdeII GATC 3 cut(s) 25, 271, 1364
NlaIII CATG 8 cut(s) 26, 103, 208, 368, 474, 556, 586, 1293
NlaIV GGNNCC 5 cut(s) 69, 97, 273, 511, 1219
NmeAIII GCCGAG 1 cut(s) 597
NmuCI GTSAC 6 cut(s) 33, 223, 346, 528, 838, 1342
PagI TCATGA 1 cut(s) 204
PctI GAATGC 1 cut(s) 1319
PdmI GAANNNNTTC 1 cut(s) 1124
PfeI GAWTC 5 cut(s) 77, 427, 766, 907, 1120
PflMI CCANNNNNTGG 3 cut(s) 1019, 1204, 1302
PkrI GCNGC 6 cut(s) 339, 409, 577, 691, 863, 883
PluTI GGCGCC 1 cut(s) 1221
PpuMI RGGWCCY 1 cut(s) 297
PsiI TTATAA 1 cut(s) 1382
Psp5II RGGWCCY 1 cut(s) 297
Psp6I CCWGG 2 cut(s) 156, 161
PspEI GGTNACC 1 cut(s) 223
PspGI CCWGG 2 cut(s) 156, 161
PspN4I GGNNCC 5 cut(s) 69, 97, 273, 511, 1219
PspPI GGNCC 6 cut(s) 96, 194, 297, 816, 1233, 1293
PspPPI RGGWCCY 1 cut(s) 297
PsrI GAACNNNNNNTAC 2 cut(s) 1064, 1096
PstNI CAGNNNCTG 1 cut(s) 350
PsuI RGATCY 1 cut(s) 271
RsaI GTAC 6 cut(s) 323, 363, 475, 516, 1265, 1272
RsaNI GTAC 6 cut(s) 322, 362, 474, 515, 1264, 1271
SaqAI TTAA 3 cut(s) 651, 704, 779
SatI GCNGC 6 cut(s) 338, 408, 576, 690, 862, 882
Sau3AI GATC 3 cut(s) 25, 271, 1364
Sau96I GGNCC 6 cut(s) 96, 194, 297, 816, 1233, 1293
ScrFI CCNGG 4 cut(s) 158, 163, 269, 903
SfaNI GCATC 1 cut(s) 469
SfcI CTRYAG 4 cut(s) 259, 1003, 1164, 1254
SfoI GGCGCC 1 cut(s) 1219
SinI GGWCC 4 cut(s) 96, 194, 297, 1293
Sse9I AATT 5 cut(s) 387, 695, 717, 1170, 1281
SsiI CCGC 1 cut(s) 864
SspDI GGCGCC 1 cut(s) 1217
SspMI CTAG 3 cut(s) 179, 681, 1399
StyD4I CCNGG 4 cut(s) 156, 161, 267, 901
StyI CCWWGG 2 cut(s) 46, 1236
TaaI ACNGT 6 cut(s) 346, 443, 1061, 1147, 1168, 1348
TaiI ACGT 1 cut(s) 779
TaqII GACCGA 1 cut(s) 211
TasI AATT 5 cut(s) 387, 695, 717, 1170, 1281
TatI WGTACW 5 cut(s) 321, 361, 473, 1263, 1270
TfiI GAWTC 5 cut(s) 77, 427, 766, 907, 1120
Tru1I TTAA 3 cut(s) 651, 704, 779
Tru9I TTAA 3 cut(s) 651, 704, 779
TscAI CASTG 5 cut(s) 79, 351, 388, 421, 1066
TseFI GTSAC 6 cut(s) 33, 223, 346, 528, 838, 1342
TseI GCWGC 6 cut(s) 337, 407, 575, 689, 861, 881
Tsp45I GTSAC 6 cut(s) 33, 223, 346, 528, 838, 1342
TspDTI ATGAA 4 cut(s) 459, 569, 824, 968
TspRI CASTG 5 cut(s) 79, 351, 388, 421, 1066
Van91I CCANNNNNTGG 3 cut(s) 1019, 1204, 1302
VpaK11BI GGWCC 4 cut(s) 96, 194, 297, 1293
XmnI GAANNNNTTC 1 cut(s) 1124
XspI CTAG 3 cut(s) 179, 681, 1399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.