Rh5AG493000

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002116
Physical Location & Seq
Reverse (-)
83898623 .. 83903649
5027 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG493000.1

Sequence Viewer

Length: 1404 bp
ATGGGTGGGGAAGAAGCAGAACAACATGATCAGCAGTCACCATTGCTCCTTGGCTTTCCTTCAACAATGGAACCCAGTGAATCTATCAAAAGAACCGGGACCATGTGGACGGCAATAGCACATATAATAACAGGAGTAATAGGGGCAGGGGTGTTATCCCTGGCCTGGAGTGTGGCTCAACTAGGGTGGATTGCAGGTCCATTGCTCATGATAGTCTTTGCTTCGGTCACCATCGTCTCCAACAACATTCTCTCTGACTGCTACAGATACCCGGATCCATATGCCCCGACCCGAAACAGGTCCTATTTGGAAGCTGTGAAGCTGTACTTAGGAGACAAGCTGCAAACAGTGACTGCTGTATTTGTACATGAGAGCTTGTATGGCAGTGGAATTGCCTATACCATTACAGCTGCTGGCAGTGTCAAAGCGATTCTGAAGTCAAACTGTTATCACAAGGAAGGGCACCAGGCTTCATGTACATATGGGGATACTCTGTATATGCTGATATTTGGAGCCGTACAGATTGTTGTGTCACAAATACCAGATTTCCATAACATGAAATGGCTTTCGGTTATTGCAGCAATCATGTCCTTTGCCTACTCTTTTATTGGACTTGGCCTCGGCTTTGCCAAAGTAATAGAAAATGGGAGTGTTAAAGGGAGCTTGTCAGGAGTTGCAACCTCTAGTTTTGCTGCTAAATTATGGTTAATCTTCCAAGCAATTGGGGACATTGCTTTTGCCTATCCATACTCTATTATTCTTTTTGAGATTCAGGATACGTTAAAGTCCCCTCCAGCAGAAAACTACACGATGAAGAGGGCCTCTGTGATTGCCATCATTGTCACAACTTTTTTCTACCTCTGCTGCGGTTGCTTTGGATATGCAGCCTTTGGGGATGACACGCCGGGGAATCTCTTGACTGGATTTGGGTTTTACGAGCCTTACTGGCTCATTGATTTTGCTAATGCTTGCATAGTTCTTCATTTGGTGGGAGGATATCAGGTCTATAGTCAGCCAGTATTTGGAGTTGCTGAGAGATGGTTTAGCAAGAAGTATCCAAACAGTGCATTTGTGAACAACTTTTACAGCTTCAAAATCCCAATGTTTGCGAGATTTCAAGTGAATCCCTTCAGGCTATGTTTCCGGACTGTTTATGTCATTTCAACTACAGTAATTGCAATCCTCTTTCCTTATTTCAACCAAGTTTTGGGAGTGTTAGGCGCCTTGAACTTTTGGCCCTTGGCTATATTTTTTCCTGTAGAGATGTACTTTGTACAAAAGAAAATTGGGACATGGACCAAGAGATGGATAGTATTACAGGCATTCAGTTTTGTTTGCTTTCTTGTGACAGTTATGGGTGTCATTGGATCAGTTGAAGGACTTATAAGTGCCAAACTTAGCTAG

Protein Analysis

467

Amino Acids

51.57

Weight (kDa)

8.24

Isoelectric Point (pI)

33.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 31 - 462 1.5e-100 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1385
Acc36I ACCTGC 1 cut(s) 185
AccB1I GGYRCC 2 cut(s) 462, 1220
AccB7I CCANNNNNTGG 3 cut(s) 1022, 1207, 1305
AccIII TCCGGA 1 cut(s) 1143
AciI CCGC 1 cut(s) 867
AclWI GGATC 3 cut(s) 269, 282, 1375
AcuI CTGAAG 2 cut(s) 455, 1114
AcyI GRCGYC 1 cut(s) 1221
AfaI GTAC 6 cut(s) 326, 366, 478, 519, 1268, 1275
AfiI CCNNNNNNNGG 5 cut(s) 165, 297, 1022, 1207, 1305
AgsI TTSAA 7 cut(s) 63, 1093, 1118, 1164, 1198, 1228, 1376
AjnI CCWGG 3 cut(s) 159, 164, 465
AluBI AGCT 8 cut(s) 314, 322, 340, 375, 410, 663, 1089, 1401
AluI AGCT 8 cut(s) 314, 322, 340, 375, 410, 663, 1089, 1401
Alw26I GTCTC 2 cut(s) 241, 327
AlwI GGATC 3 cut(s) 269, 282, 1375
AlwNI CAGNNNCTG 2 cut(s) 353, 413
Aor13HI TCCGGA 1 cut(s) 1143
AoxI GGCC 4 cut(s) 162, 616, 819, 1235
ApeKI GCWGC 6 cut(s) 340, 410, 578, 692, 864, 884
ArsI GACNNNNNNTTYG 2 cut(s) 719, 751
Asp700I GAANNNNTTC 1 cut(s) 1127
AspLEI GCGC 1 cut(s) 1223
AspS9I GGNCC 6 cut(s) 99, 197, 300, 819, 1236, 1296
AsuC2I CCSGG 3 cut(s) 97, 272, 906
AsuHPI GGTGA 2 cut(s) 30, 220
AvaII GGWCC 4 cut(s) 99, 197, 300, 1296
BaeGI GKGCMC 1 cut(s) 465
BamHI GGATCC 1 cut(s) 274
BanI GGYRCC 2 cut(s) 462, 1220
BbvI GCAGC 6 cut(s) 327, 397, 590, 679, 851, 896
BccI CCATC 4 cut(s) 239, 842, 1032, 1299
BceAI ACGGC 2 cut(s) 126, 500
BciT130I CCWGG 3 cut(s) 161, 166, 467
BciVI GTATCC 3 cut(s) 481, 769, 1065
BclI TGATCA 1 cut(s) 28
BcnI CCSGG 3 cut(s) 97, 272, 906
BcoDI GTCTC 2 cut(s) 241, 327
BfaI CTAG 3 cut(s) 182, 684, 1402
BfmI CTRYAG 4 cut(s) 262, 1006, 1167, 1257
BfoI RGCGCY 1 cut(s) 1224
BfuAI ACCTGC 1 cut(s) 185
BfuI GTATCC 3 cut(s) 481, 769, 1065
BglI GCCNNNNNGGC 1 cut(s) 946
BisI GCNGC 6 cut(s) 341, 411, 579, 693, 865, 885
BlsI GCNGC 6 cut(s) 342, 412, 580, 694, 866, 886
Bme1390I CCNGG 6 cut(s) 97, 161, 166, 272, 467, 906
Bme18I GGWCC 4 cut(s) 99, 197, 300, 1296
BmgT120I GGNCC 6 cut(s) 99, 197, 300, 819, 1236, 1296
BmiI GGNNCC 6 cut(s) 72, 100, 276, 464, 514, 1222
BmrFI CCNGG 6 cut(s) 97, 161, 166, 272, 467, 906
BmrI ACTGGG 1 cut(s) 69
BmuI ACTGGG 1 cut(s) 69
BplI GAGNNNNNCTC 2 cut(s) 160, 192
BpmI CTGGAG 2 cut(s) 187, 777
BpuMI CCSGG 3 cut(s) 97, 272, 906
BsaBI GATNNNNATC 1 cut(s) 833
BsaHI GRCGYC 1 cut(s) 1221
BsaJI CCNNGG 5 cut(s) 49, 159, 619, 905, 1239
BsaWI WCCGGW 1 cut(s) 1143
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bsc4I CCNNNNNNNGG 5 cut(s) 165, 297, 1022, 1207, 1305
Bse1I ACTGG 4 cut(s) 75, 925, 950, 1016
Bse3DI GCAATG 3 cut(s) 41, 200, 729
Bse8I GATNNNNATC 1 cut(s) 833
BseAI TCCGGA 1 cut(s) 1143
BseBI CCWGG 3 cut(s) 161, 166, 467
BseDI CCNNGG 5 cut(s) 49, 159, 619, 905, 1239
BseGI GGATG 1 cut(s) 901
BseJI GATNNNNATC 1 cut(s) 833
BseLI CCNNNNNNNGG 5 cut(s) 165, 297, 1022, 1207, 1305
BseMI GCAATG 3 cut(s) 41, 200, 729
BseMII CTCAG 1 cut(s) 1023
BseNI ACTGG 4 cut(s) 75, 925, 950, 1016
BseSI GKGCMC 1 cut(s) 465
BseXI GCAGC 6 cut(s) 327, 397, 590, 679, 851, 896
BshFI GGCC 4 cut(s) 164, 618, 821, 1237
BshNI GGYRCC 2 cut(s) 462, 1220
BsiSI CCGG 4 cut(s) 96, 272, 905, 1144
BslFI GGGAC 4 cut(s) 112, 740, 772, 1303
BslI CCNNNNNNNGG 5 cut(s) 165, 297, 1022, 1207, 1305
BsmAI GTCTC 2 cut(s) 241, 327
BsmBI CGTCTC 1 cut(s) 241
BsmFI GGGAC 4 cut(s) 112, 740, 772, 1303
BsmI GAATGC 1 cut(s) 1322
BsnI GGCC 4 cut(s) 164, 618, 821, 1237
Bsp1286I GDGCHC 1 cut(s) 465
Bsp13I TCCGGA 1 cut(s) 1143
Bsp1407I TGTACA 3 cut(s) 364, 476, 1273
Bsp143I GATC 3 cut(s) 28, 274, 1367
BspACI CCGC 1 cut(s) 867
BspANI GGCC 4 cut(s) 164, 618, 821, 1237
BspCNI CTCAG 1 cut(s) 1024
BspEI TCCGGA 1 cut(s) 1143
BspHI TCATGA 1 cut(s) 207
BspLI GGNNCC 6 cut(s) 72, 100, 276, 464, 514, 1222
BspMI ACCTGC 1 cut(s) 185
BspPI GGATC 3 cut(s) 269, 282, 1375
BspT107I GGYRCC 2 cut(s) 462, 1220
BsrDI GCAATG 3 cut(s) 41, 200, 729
BsrGI TGTACA 3 cut(s) 364, 476, 1273
BsrI ACTGG 4 cut(s) 75, 925, 950, 1016
BssECI CCNNGG 5 cut(s) 49, 159, 619, 905, 1239
BssMI GATC 3 cut(s) 28, 274, 1367
BssNI GRCGYC 1 cut(s) 1221
BssT1I CCWWGG 2 cut(s) 49, 1239
Bst2UI CCWGG 3 cut(s) 161, 166, 467
Bst4CI ACNGT 6 cut(s) 349, 446, 1064, 1150, 1171, 1351
Bst6I CTCTTC 1 cut(s) 809
BstACI GRCGYC 1 cut(s) 1221
BstAUI TGTACA 3 cut(s) 364, 476, 1273
BstC8I GCNNGC 2 cut(s) 415, 970
BstDEI CTNAG 3 cut(s) 328, 1032, 1397
BstEII GGTNACC 1 cut(s) 226
BstF5I GGATG 1 cut(s) 901
BstH2I RGCGCY 1 cut(s) 1224
BstHHI GCGC 1 cut(s) 1223
BstKTI GATC 3 cut(s) 31, 277, 1370
BstMAI GTCTC 2 cut(s) 241, 327
BstMBI GATC 3 cut(s) 28, 274, 1367
BstMWI GCNNNNNNNGC 3 cut(s) 381, 870, 946
BstNI CCWGG 3 cut(s) 161, 166, 467
BstPI GGTNACC 1 cut(s) 226
BstSCI CCNGG 6 cut(s) 95, 159, 164, 270, 465, 904
BstSFI CTRYAG 4 cut(s) 262, 1006, 1167, 1257
BstSLI GKGCMC 1 cut(s) 465
BstV1I GCAGC 6 cut(s) 327, 397, 590, 679, 851, 896
BstX2I RGATCY 1 cut(s) 274
BstXI CCANNNNNNTGG 1 cut(s) 722
BstYI RGATCY 1 cut(s) 274
BsuI GTATCC 3 cut(s) 481, 769, 1065
BsuRI GGCC 4 cut(s) 164, 618, 821, 1237
BtsCI GGATG 1 cut(s) 901
BtsI GCAGTG 2 cut(s) 391, 424
BtsIMutI CAGTG 5 cut(s) 82, 354, 391, 424, 1069
BveI ACCTGC 1 cut(s) 185
Cac8I GCNNGC 2 cut(s) 415, 970
CaiI CAGNNNCTG 2 cut(s) 353, 413
CciI TCATGA 1 cut(s) 207
CfoI GCGC 1 cut(s) 1223
Cfr13I GGNCC 6 cut(s) 99, 197, 300, 819, 1236, 1296
Csp6I GTAC 6 cut(s) 325, 365, 477, 518, 1267, 1274
CspCI CAANNNNNGTGG 2 cut(s) 167, 202
CviAII CATG 8 cut(s) 26, 103, 208, 368, 474, 556, 586, 1293
CviQI GTAC 6 cut(s) 325, 365, 477, 518, 1267, 1274
DdeI CTNAG 3 cut(s) 328, 1032, 1397
DinI GGCGCC 1 cut(s) 1222
DpnI GATC 3 cut(s) 30, 276, 1369
DpnII GATC 3 cut(s) 28, 274, 1367
Eam1104I CTCTTC 1 cut(s) 809
EarI CTCTTC 1 cut(s) 809
Eco130I CCWWGG 2 cut(s) 49, 1239
Eco32I GATATC 1 cut(s) 998
Eco47I GGWCC 4 cut(s) 99, 197, 300, 1296
Eco57I CTGAAG 2 cut(s) 455, 1114
Eco91I GGTNACC 1 cut(s) 226
EcoO109I RGGNCCY 2 cut(s) 300, 819
EcoO65I GGTNACC 1 cut(s) 226
EcoRII CCWGG 3 cut(s) 159, 164, 465
EcoRV GATATC 1 cut(s) 998
EcoT14I CCWWGG 2 cut(s) 49, 1239
EgeI GGCGCC 1 cut(s) 1222
EheI GGCGCC 1 cut(s) 1222
ErhI CCWWGG 2 cut(s) 49, 1239
Esp3I CGTCTC 1 cut(s) 241
FaeI CATG 8 cut(s) 29, 106, 211, 371, 477, 559, 589, 1296
FalI AAGNNNNNCTT 2 cut(s) 311, 343
FaqI GGGAC 4 cut(s) 112, 740, 772, 1303
FatI CATG 8 cut(s) 25, 102, 207, 367, 473, 555, 585, 1292
FauNDI CATATG 2 cut(s) 280, 481
FbaI TGATCA 1 cut(s) 28
Fnu4HI GCNGC 6 cut(s) 341, 411, 579, 693, 865, 885
FokI GGATG 1 cut(s) 908
Fsp4HI GCNGC 6 cut(s) 341, 411, 579, 693, 865, 885
FspBI CTAG 3 cut(s) 182, 684, 1402
GlaI GCGC 1 cut(s) 1222
GluI GCNGC 6 cut(s) 341, 411, 579, 693, 865, 885
GsuI CTGGAG 2 cut(s) 187, 777
HaeII RGCGCY 1 cut(s) 1224
HaeIII GGCC 4 cut(s) 164, 618, 821, 1237
HapII CCGG 4 cut(s) 96, 272, 905, 1144
HhaI GCGC 1 cut(s) 1223
Hin1I GRCGYC 1 cut(s) 1221
Hin1II CATG 8 cut(s) 29, 106, 211, 371, 477, 559, 589, 1296
Hin6I GCGC 1 cut(s) 1221
HinP1I GCGC 1 cut(s) 1221
HinfI GANTC 5 cut(s) 80, 430, 769, 910, 1123
HpaII CCGG 4 cut(s) 96, 272, 905, 1144
HphI GGTGA 2 cut(s) 30, 220
Hpy166II GTNNAC 2 cut(s) 108, 1075
Hpy188I TCNGA 2 cut(s) 256, 435
Hpy188III TCNNGA 5 cut(s) 208, 669, 773, 916, 1144
Hpy8I GTNNAC 2 cut(s) 108, 1075
HpyAV CCTTC 4 cut(s) 69, 452, 1138, 1370
HpyCH4III ACNGT 6 cut(s) 349, 446, 1064, 1150, 1171, 1351
HpyCH4IV ACGT 1 cut(s) 779
HpyCH4V TGCA 8 cut(s) 194, 343, 578, 677, 884, 972, 1067, 1178
HpyF10VI GCNNNNNNNGC 3 cut(s) 381, 870, 946
HpyF3I CTNAG 3 cut(s) 328, 1032, 1397
HpySE526I ACGT 1 cut(s) 779
Hsp92I GRCGYC 1 cut(s) 1221
Hsp92II CATG 8 cut(s) 29, 106, 211, 371, 477, 559, 589, 1296
HspAI GCGC 1 cut(s) 1221
KasI GGCGCC 1 cut(s) 1220
Kpn2I TCCGGA 1 cut(s) 1143
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 3 cut(s) 28, 274, 1367
LmnI GCTCC 3 cut(s) 51, 512, 660
Lsp1109I GCAGC 6 cut(s) 327, 397, 590, 679, 851, 896
MaeI CTAG 3 cut(s) 182, 684, 1402
MaeII ACGT 1 cut(s) 779
MaeIII GTNAC 6 cut(s) 36, 226, 349, 531, 841, 1345
MalI GATC 3 cut(s) 30, 276, 1369
MboI GATC 3 cut(s) 28, 274, 1367
MboII GAAGA 4 cut(s) 23, 703, 826, 971
MfeI CAATTG 1 cut(s) 720
MflI RGATCY 1 cut(s) 274
MhlI GDGCHC 1 cut(s) 465
MluCI AATT 5 cut(s) 390, 698, 720, 1173, 1284
Mly113I GGCGCC 1 cut(s) 1221
MmeI TCCRAC 1 cut(s) 264
MnlI CCTC 8 cut(s) 629, 691, 801, 810, 832, 869, 986, 1193
MroI TCCGGA 1 cut(s) 1143
MroXI GAANNNNTTC 1 cut(s) 1127
MseI TTAA 3 cut(s) 654, 707, 782
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 4 cut(s) 96, 272, 905, 1144
MspR9I CCNGG 6 cut(s) 97, 161, 166, 272, 467, 906
MunI CAATTG 1 cut(s) 720
Mva1269I GAATGC 1 cut(s) 1322
MvaI CCWGG 3 cut(s) 161, 166, 467
MwoI GCNNNNNNNGC 3 cut(s) 381, 870, 946
NarI GGCGCC 1 cut(s) 1221
NciI CCSGG 3 cut(s) 97, 272, 906
NdeI CATATG 2 cut(s) 280, 481
NdeII GATC 3 cut(s) 28, 274, 1367
NlaIII CATG 8 cut(s) 29, 106, 211, 371, 477, 559, 589, 1296
NlaIV GGNNCC 6 cut(s) 72, 100, 276, 464, 514, 1222
NmeAIII GCCGAG 1 cut(s) 600
NmuCI GTSAC 6 cut(s) 36, 226, 349, 531, 841, 1345
PagI TCATGA 1 cut(s) 207
PctI GAATGC 1 cut(s) 1322
PdmI GAANNNNTTC 1 cut(s) 1127
PfeI GAWTC 5 cut(s) 80, 430, 769, 910, 1123
PflMI CCANNNNNTGG 3 cut(s) 1022, 1207, 1305
PkrI GCNGC 6 cut(s) 342, 412, 580, 694, 866, 886
PluTI GGCGCC 1 cut(s) 1224
PpuMI RGGWCCY 1 cut(s) 300
PsiI TTATAA 1 cut(s) 1385
Psp5II RGGWCCY 1 cut(s) 300
Psp6I CCWGG 3 cut(s) 159, 164, 465
PspEI GGTNACC 1 cut(s) 226
PspGI CCWGG 3 cut(s) 159, 164, 465
PspN4I GGNNCC 6 cut(s) 72, 100, 276, 464, 514, 1222
PspPI GGNCC 6 cut(s) 99, 197, 300, 819, 1236, 1296
PspPPI RGGWCCY 1 cut(s) 300
PsrI GAACNNNNNNTAC 2 cut(s) 1067, 1099
PstNI CAGNNNCTG 2 cut(s) 353, 413
PsuI RGATCY 1 cut(s) 274
PvuII CAGCTG 1 cut(s) 410
RsaI GTAC 6 cut(s) 326, 366, 478, 519, 1268, 1275
RsaNI GTAC 6 cut(s) 325, 365, 477, 518, 1267, 1274
SaqAI TTAA 3 cut(s) 654, 707, 782
SatI GCNGC 6 cut(s) 341, 411, 579, 693, 865, 885
Sau3AI GATC 3 cut(s) 28, 274, 1367
Sau96I GGNCC 6 cut(s) 99, 197, 300, 819, 1236, 1296
ScrFI CCNGG 6 cut(s) 97, 161, 166, 272, 467, 906
SduI GDGCHC 1 cut(s) 465
SfcI CTRYAG 4 cut(s) 262, 1006, 1167, 1257
SfoI GGCGCC 1 cut(s) 1222
SinI GGWCC 4 cut(s) 99, 197, 300, 1296
Sse9I AATT 5 cut(s) 390, 698, 720, 1173, 1284
SsiI CCGC 1 cut(s) 867
SspDI GGCGCC 1 cut(s) 1220
SspMI CTAG 3 cut(s) 182, 684, 1402
StyD4I CCNGG 6 cut(s) 95, 159, 164, 270, 465, 904
StyI CCWWGG 2 cut(s) 49, 1239
TaaI ACNGT 6 cut(s) 349, 446, 1064, 1150, 1171, 1351
TaiI ACGT 1 cut(s) 782
TaqII GACCGA 1 cut(s) 214
TasI AATT 5 cut(s) 390, 698, 720, 1173, 1284
TatI WGTACW 5 cut(s) 324, 364, 476, 1266, 1273
TfiI GAWTC 5 cut(s) 80, 430, 769, 910, 1123
Tru1I TTAA 3 cut(s) 654, 707, 782
Tru9I TTAA 3 cut(s) 654, 707, 782
TscAI CASTG 5 cut(s) 82, 354, 391, 424, 1069
TseFI GTSAC 6 cut(s) 36, 226, 349, 531, 841, 1345
TseI GCWGC 6 cut(s) 340, 410, 578, 692, 864, 884
Tsp45I GTSAC 6 cut(s) 36, 226, 349, 531, 841, 1345
TspDTI ATGAA 4 cut(s) 462, 572, 827, 971
TspRI CASTG 5 cut(s) 82, 354, 391, 424, 1069
Van91I CCANNNNNTGG 3 cut(s) 1022, 1207, 1305
VpaK11BI GGWCC 4 cut(s) 99, 197, 300, 1296
XmnI GAANNNNTTC 1 cut(s) 1127
XspI CTAG 3 cut(s) 182, 684, 1402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.