MD03G1144300.v1.1

Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003912
Physical Location & Seq
Forward (+)
15521124 .. 15522648
1525 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1144300.v1.1.491

Sequence Viewer

Length: 555 bp
ATGATGATGTTTATGTTGATTCACCAGATTCTTTTCTGTGCTTTTGCTTTTACTAATCTTCTTCTTCACTGGTGTATGAGTTCAGGGGTAAACAAGTCAGCCATGACAACCGTGCAAGTTCCCTCCCAATGCATGTTCAGACATGCGCCCAAAAAGTGGTTCACCAGTTCAATTGTGAAGTTATCTTCTCCTCTTGGATCTGTGAAGAATATCTCAAAGTCCTTTGGCTTGAAAGCATCCTCCAACTCCCGGGCATCAATGGAAGTATACAAGGTTAAATTGATTGGACCAGATGGCGTGGAGAACGAATTTGAGGCGGCGGATGATTGTTACATCTTAGATGCAGCTGAGAATGCCGGAGTTGAGCTGCCATATTCTTGCAGGGCTGGGGCATGCTCTACCTGTGCGGGGAAGCTGGCATCAGGTTCAGTGGATCAGTCCGATGGTTCATTCCTCGACGACAATCAAATGAAGGAGGGTTATGTGCTGACTTGTGTCTCGTACCCGACTTCAGACCTTGTGATTCACACTCACAAGGAAAGTGAGCTGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 map00195
Pfam Domains
Protein Families
Fer2

Protein Analysis

185

Amino Acids

20.1

Weight (kDa)

5.92

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 97 - 170 1e-22 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 156
AccI GTMKAC 1 cut(s) 267
AciI CCGC 3 cut(s) 317, 320, 407
AclWI GGATC 2 cut(s) 205, 441
AcsI RAATTY 1 cut(s) 308
AcuI CTGAAG 1 cut(s) 495
AfaI GTAC 1 cut(s) 503
AfiI CCNNNNNNNGG 3 cut(s) 156, 249, 408
AgsI TTSAA 2 cut(s) 171, 232
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
AluBI AGCT 4 cut(s) 347, 367, 415, 547
AluI AGCT 4 cut(s) 347, 367, 415, 547
Alw26I GTCTC 1 cut(s) 502
AlwI GGATC 2 cut(s) 205, 441
Ama87I CYCGRG 1 cut(s) 249
ApeKI GCWGC 2 cut(s) 344, 367
ApoI RAATTY 1 cut(s) 308
AspLEI GCGC 1 cut(s) 148
AspS9I GGNCC 1 cut(s) 287
AsuC2I CCSGG 2 cut(s) 250, 251
AsuHPI GGTGA 2 cut(s) 14, 154
AvaI CYCGRG 1 cut(s) 249
AvaII GGWCC 1 cut(s) 287
BbvI GCAGC 2 cut(s) 354, 356
BccI CCATC 2 cut(s) 287, 437
BcnI CCSGG 2 cut(s) 250, 251
BcoDI GTCTC 1 cut(s) 502
BisI GCNGC 3 cut(s) 318, 345, 368
BlsI GCNGC 3 cut(s) 319, 346, 369
Bme1390I CCNGG 2 cut(s) 250, 251
Bme18I GGWCC 1 cut(s) 287
BmeT110I CYCGRG 1 cut(s) 249
BmgT120I GGNCC 1 cut(s) 287
BmrFI CCNGG 2 cut(s) 250, 251
BmsI GCATC 4 cut(s) 245, 263, 331, 428
BoxI GACNNNNGTC 1 cut(s) 494
BpuMI CCSGG 2 cut(s) 250, 251
BsaJI CCNNGG 1 cut(s) 249
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 249, 408
Bse1I ACTGG 2 cut(s) 74, 165
BseDI CCNNGG 1 cut(s) 249
BseGI GGATG 2 cut(s) 236, 328
BseLI CCNNNNNNNGG 3 cut(s) 156, 249, 408
BseMII CTCAG 1 cut(s) 339
BseNI ACTGG 2 cut(s) 74, 165
BseRI GAGGAG 1 cut(s) 180
BseXI GCAGC 2 cut(s) 354, 356
BseYI CCCAGC 1 cut(s) 386
BsiHKCI CYCGRG 1 cut(s) 249
BsiSI CCGG 2 cut(s) 250, 357
BslI CCNNNNNNNGG 3 cut(s) 156, 249, 408
BsmAI GTCTC 1 cut(s) 502
BsmI GAATGC 1 cut(s) 358
BsoBI CYCGRG 1 cut(s) 249
Bsp143I GATC 2 cut(s) 197, 433
BspACI CCGC 3 cut(s) 317, 320, 407
BspCNI CTCAG 1 cut(s) 340
BspPI GGATC 2 cut(s) 205, 441
BsrI ACTGG 2 cut(s) 74, 165
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 2 cut(s) 197, 433
BssNAI GTATAC 1 cut(s) 268
Bst1107I GTATAC 1 cut(s) 268
Bst4CI ACNGT 1 cut(s) 112
BstC8I GCNNGC 2 cut(s) 394, 417
BstDEI CTNAG 2 cut(s) 337, 348
BstF5I GGATG 2 cut(s) 236, 328
BstHHI GCGC 1 cut(s) 148
BstKTI GATC 2 cut(s) 200, 436
BstMAI GTCTC 1 cut(s) 502
BstMBI GATC 2 cut(s) 197, 433
BstMWI GCNNNNNNNGC 1 cut(s) 353
BstNSI RCATGY 3 cut(s) 136, 146, 396
BstPAI GACNNNNGTC 1 cut(s) 494
BstSCI CCNGG 2 cut(s) 248, 249
BstV1I GCAGC 2 cut(s) 354, 356
BstX2I RGATCY 1 cut(s) 197
BstYI RGATCY 1 cut(s) 197
BstZ17I GTATAC 1 cut(s) 268
BtsCI GGATG 2 cut(s) 236, 328
BtsIMutI CAGTG 2 cut(s) 67, 435
Cac8I GCNNGC 2 cut(s) 394, 417
CfoI GCGC 1 cut(s) 148
Cfr13I GGNCC 1 cut(s) 287
Cfr9I CCCGGG 1 cut(s) 249
Csp6I GTAC 1 cut(s) 502
CviAII CATG 4 cut(s) 103, 133, 143, 393
CviJI RGCY 7 cut(s) 101, 228, 347, 367, 386, 415, 547
CviKI_1 RGCY 7 cut(s) 101, 228, 347, 367, 386, 415, 547
CviQI GTAC 1 cut(s) 502
DdeI CTNAG 2 cut(s) 337, 348
DpnI GATC 2 cut(s) 199, 435
DpnII GATC 2 cut(s) 197, 433
EciI GGCGGA 1 cut(s) 335
Eco47I GGWCC 1 cut(s) 287
Eco57I CTGAAG 1 cut(s) 495
Eco88I CYCGRG 1 cut(s) 249
EcoT22I ATGCAT 1 cut(s) 134
FaeI CATG 4 cut(s) 106, 136, 146, 396
FaiI YATR 9 cut(s) 14, 77, 104, 134, 144, 268, 373, 394, 483
FatI CATG 4 cut(s) 102, 132, 142, 392
FauI CCCGC 1 cut(s) 400
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 3 cut(s) 318, 345, 368
FokI GGATG 2 cut(s) 223, 335
Fsp4HI GCNGC 3 cut(s) 318, 345, 368
GlaI GCGC 1 cut(s) 147
GluI GCNGC 3 cut(s) 318, 345, 368
GsaI CCCAGC 1 cut(s) 390
HapII CCGG 2 cut(s) 250, 357
HhaI GCGC 1 cut(s) 148
Hin1II CATG 4 cut(s) 106, 136, 146, 396
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HinfI GANTC 3 cut(s) 19, 28, 523
HpaII CCGG 2 cut(s) 250, 357
HphI GGTGA 2 cut(s) 14, 154
Hpy166II GTNNAC 3 cut(s) 91, 162, 268
Hpy188I TCNGA 3 cut(s) 140, 442, 514
Hpy8I GTNNAC 3 cut(s) 91, 162, 268
Hpy99I CGWCG 1 cut(s) 461
HpyAV CCTTC 1 cut(s) 466
HpyCH4III ACNGT 1 cut(s) 112
HpyCH4V TGCA 4 cut(s) 115, 132, 344, 381
HpyF10VI GCNNNNNNNGC 1 cut(s) 353
HpyF3I CTNAG 2 cut(s) 337, 348
Hsp92II CATG 4 cut(s) 106, 136, 146, 396
HspAI GCGC 1 cut(s) 146
Kzo9I GATC 2 cut(s) 197, 433
Lsp1109I GCAGC 2 cut(s) 354, 356
LweI GCATC 4 cut(s) 245, 263, 331, 428
MaeIII GTNAC 1 cut(s) 329
MalI GATC 2 cut(s) 199, 435
MboI GATC 2 cut(s) 197, 433
MboII GAAGA 5 cut(s) 50, 53, 56, 177, 217
MfeI CAATTG 1 cut(s) 171
MflI RGATCY 1 cut(s) 197
MluCI AATT 3 cut(s) 171, 278, 308
MmeI TCCRAC 1 cut(s) 267
MnlI CCTC 6 cut(s) 133, 201, 250, 307, 464, 469
Mph1103I ATGCAT 1 cut(s) 134
MseI TTAA 1 cut(s) 276
MspA1I CMGCKG 1 cut(s) 347
MspI CCGG 2 cut(s) 250, 357
MspR9I CCNGG 2 cut(s) 250, 251
MunI CAATTG 1 cut(s) 171
Mva1269I GAATGC 1 cut(s) 358
MwoI GCNNNNNNNGC 1 cut(s) 353
NciI CCSGG 2 cut(s) 250, 251
NdeII GATC 2 cut(s) 197, 433
NlaIII CATG 4 cut(s) 106, 136, 146, 396
NsiI ATGCAT 1 cut(s) 134
NspI RCATGY 3 cut(s) 136, 146, 396
PaeI GCATGC 1 cut(s) 396
PctI GAATGC 1 cut(s) 358
PfeI GAWTC 3 cut(s) 19, 28, 523
PflMI CCANNNNNTGG 1 cut(s) 156
PkrI GCNGC 3 cut(s) 319, 346, 369
PshAI GACNNNNGTC 1 cut(s) 494
PspFI CCCAGC 1 cut(s) 386
PspPI GGNCC 1 cut(s) 287
PsuI RGATCY 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 347
RsaI GTAC 1 cut(s) 503
RsaNI GTAC 1 cut(s) 502
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 3 cut(s) 318, 345, 368
Sau3AI GATC 2 cut(s) 197, 433
Sau96I GGNCC 1 cut(s) 287
ScrFI CCNGG 2 cut(s) 250, 251
SetI ASST 8 cut(s) 276, 349, 369, 404, 417, 427, 519, 549
SfaNI GCATC 4 cut(s) 245, 263, 331, 428
SinI GGWCC 1 cut(s) 287
SmaI CCCGGG 1 cut(s) 251
SphI GCATGC 1 cut(s) 396
Sse9I AATT 3 cut(s) 171, 278, 308
SsiI CCGC 3 cut(s) 317, 320, 407
StyD4I CCNGG 2 cut(s) 248, 249
TaaI ACNGT 1 cut(s) 112
TaqI TCGA 1 cut(s) 456
TasI AATT 3 cut(s) 171, 278, 308
TauI GCSGC 1 cut(s) 320
TfiI GAWTC 3 cut(s) 19, 28, 523
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TscAI CASTG 2 cut(s) 74, 435
TseI GCWGC 2 cut(s) 344, 367
TspDTI ATGAA 2 cut(s) 438, 485
TspMI CCCGGG 1 cut(s) 249
TspRI CASTG 2 cut(s) 74, 435
Van91I CCANNNNNTGG 1 cut(s) 156
VpaK11BI GGWCC 1 cut(s) 287
XapI RAATTY 1 cut(s) 308
XceI RCATGY 3 cut(s) 136, 146, 396
XmaI CCCGGG 1 cut(s) 249
XmiI GTMKAC 1 cut(s) 267
Zsp2I ATGCAT 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.