MD03G1147300.v1.1

40S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001219
Physical Location & Seq
Reverse (-)
16242716 .. 16245644
2929 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1147300.v1.1.491

Sequence Viewer

Length: 327 bp
ATGGCTCCCAAGAAGGATAAGGCTCCCCCACCGTCCTCAAAGCCTGCCAAGTCCGGAGGAGGCAAGCAGAAGAAGAAAAAGTGGAGCAAGGGAAAGCAAAAGGAGAAGGTGAACAACATGGTTTTGTTTGATCAGGGCACCTACGACAAGCTGCTTTCAGAAGTTCCGAAGTACAAACTCATTACTCCTTCCATCTTGTCTGACAGACTGAGGATTAATGGATCATTGGCTCGCCGTGCAATTAAGGATTTGATGGCAAGGGGTTCCATTAGGCTGATAGCTGCTCATGCTAGCCAGCAGATTTACACCAGAGCCACCAATACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S25

Protein Analysis

109

Amino Acids

11.98

Weight (kDa)

10.59

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S25 PF03297 7 - 105 2.8e-41 S25 ribosomal protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 137
AccIII TCCGGA 1 cut(s) 53
AclWI GGATC 1 cut(s) 229
AfaI GTAC 1 cut(s) 173
AluBI AGCT 2 cut(s) 151, 281
AluI AGCT 2 cut(s) 151, 281
AlwI GGATC 1 cut(s) 229
Aor13HI TCCGGA 1 cut(s) 53
ApeKI GCWGC 2 cut(s) 151, 281
AseI ATTAAT 1 cut(s) 216
AsuHPI GGTGA 1 cut(s) 121
AsuNHI GCTAGC 1 cut(s) 290
BaeGI GKGCMC 1 cut(s) 140
BanI GGYRCC 1 cut(s) 137
BbvI GCAGC 2 cut(s) 138, 268
BccI CCATC 2 cut(s) 200, 247
BceAI ACGGC 1 cut(s) 219
BclI TGATCA 1 cut(s) 130
BfaI CTAG 1 cut(s) 291
BisI GCNGC 2 cut(s) 152, 282
BlsI GCNGC 2 cut(s) 153, 283
BmiI GGNNCC 4 cut(s) 6, 24, 139, 265
BmtI GCTAGC 1 cut(s) 294
BsaWI WCCGGW 1 cut(s) 53
BseAI TCCGGA 1 cut(s) 53
BseMII CTCAG 1 cut(s) 200
BseRI GAGGAG 1 cut(s) 72
BseSI GKGCMC 1 cut(s) 140
BseXI GCAGC 2 cut(s) 138, 268
BshNI GGYRCC 1 cut(s) 137
BsiSI CCGG 1 cut(s) 54
Bsp1286I GDGCHC 1 cut(s) 140
Bsp13I TCCGGA 1 cut(s) 53
Bsp143I GATC 2 cut(s) 130, 221
BspCNI CTCAG 1 cut(s) 201
BspEI TCCGGA 1 cut(s) 53
BspLI GGNNCC 4 cut(s) 6, 24, 139, 265
BspOI GCTAGC 1 cut(s) 294
BspPI GGATC 1 cut(s) 229
BspT107I GGYRCC 1 cut(s) 137
BssMI GATC 2 cut(s) 130, 221
Bst4CI ACNGT 1 cut(s) 33
BstC8I GCNNGC 5 cut(s) 45, 65, 232, 292, 296
BstDEI CTNAG 1 cut(s) 209
BstKTI GATC 2 cut(s) 133, 224
BstMBI GATC 2 cut(s) 130, 221
BstMWI GCNNNNNNNGC 2 cut(s) 236, 287
BstSLI GKGCMC 1 cut(s) 140
BstV1I GCAGC 2 cut(s) 138, 268
Cac8I GCNNGC 5 cut(s) 45, 65, 232, 292, 296
Csp6I GTAC 1 cut(s) 172
CviAII CATG 2 cut(s) 118, 287
CviJI RGCY 9 cut(s) 5, 23, 43, 151, 230, 274, 281, 294, 314
CviKI_1 RGCY 9 cut(s) 5, 23, 43, 151, 230, 274, 281, 294, 314
CviQI GTAC 1 cut(s) 172
DdeI CTNAG 1 cut(s) 209
DpnI GATC 2 cut(s) 132, 223
DpnII GATC 2 cut(s) 130, 221
FaeI CATG 2 cut(s) 121, 290
FaiI YATR 2 cut(s) 119, 288
FatI CATG 2 cut(s) 117, 286
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 2 cut(s) 152, 282
Fsp4HI GCNGC 2 cut(s) 152, 282
FspBI CTAG 1 cut(s) 291
GluI GCNGC 2 cut(s) 152, 282
HapII CCGG 1 cut(s) 54
Hin1II CATG 2 cut(s) 121, 290
HpaII CCGG 1 cut(s) 54
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 1 cut(s) 112
Hpy188I TCNGA 3 cut(s) 160, 168, 202
Hpy188III TCNNGA 1 cut(s) 54
Hpy8I GTNNAC 1 cut(s) 112
HpyAV CCTTC 3 cut(s) 7, 100, 198
HpyCH4III ACNGT 1 cut(s) 33
HpyCH4V TGCA 1 cut(s) 239
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 287
HpyF3I CTNAG 1 cut(s) 209
Hsp92II CATG 2 cut(s) 121, 290
Kpn2I TCCGGA 1 cut(s) 53
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 2 cut(s) 130, 221
LmnI GCTCC 3 cut(s) 10, 28, 84
LpnPI CCDG 5 cut(s) 57, 67, 119, 308, 322
Lsp1109I GCAGC 2 cut(s) 138, 268
MaeI CTAG 1 cut(s) 291
MalI GATC 2 cut(s) 132, 223
MboI GATC 2 cut(s) 130, 221
MboII GAAGA 2 cut(s) 82, 85
MhlI GDGCHC 1 cut(s) 140
MluCI AATT 1 cut(s) 240
MnlI CCTC 4 cut(s) 46, 50, 53, 204
MroI TCCGGA 1 cut(s) 53
MseI TTAA 3 cut(s) 216, 243, 325
MspI CCGG 1 cut(s) 54
MwoI GCNNNNNNNGC 2 cut(s) 236, 287
NdeII GATC 2 cut(s) 130, 221
NheI GCTAGC 1 cut(s) 290
NlaIII CATG 2 cut(s) 121, 290
NlaIV GGNNCC 4 cut(s) 6, 24, 139, 265
PkrI GCNGC 2 cut(s) 153, 283
PshBI ATTAAT 1 cut(s) 216
PspN4I GGNNCC 4 cut(s) 6, 24, 139, 265
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
SaqAI TTAA 3 cut(s) 216, 243, 325
SatI GCNGC 2 cut(s) 152, 282
Sau3AI GATC 2 cut(s) 130, 221
SduI GDGCHC 1 cut(s) 140
SetI ASST 4 cut(s) 111, 143, 153, 283
Sse9I AATT 1 cut(s) 240
SspMI CTAG 1 cut(s) 291
TaaI ACNGT 1 cut(s) 33
TasI AATT 1 cut(s) 240
TatI WGTACW 1 cut(s) 171
Tru1I TTAA 3 cut(s) 216, 243, 325
Tru9I TTAA 3 cut(s) 216, 243, 325
TseI GCWGC 2 cut(s) 151, 281
VspI ATTAAT 1 cut(s) 216
XspI CTAG 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.