Rorug05G0223700

40S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001219
Physical Location & Seq
Forward (+)
23215142 .. 23218110
2969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0223700.1

Sequence Viewer

Length: 1002 bp
ATGGATTTGATCTGGGTTTTTTCCTTGGTGTTTCTTTATCTTGGGGTGTTCTCATTTGGGTCTAGTAATAATCTTTCTTCAAGACCGGCTCTTGTCAACATTGGAGCTCTTTTCACGTATGACTCTACGATTGGTAAGGTCGCCAAGATTGCTATTGAGGAGGCAGTAAAAGATGTCAACTCCAATTTTAGCATTCTCCATGGGACCAAACTTGTTGCGAAATTTCAGAATACCAATTGCAGTGGGTTTATTGGAATGGTTGAAGCTTTGCAATTCATGGAGACTGATATCGTTGCCATTATAGGCCCACAATCTTCTGTGGTTGCCCATATTGTATCCCACATTGCAAATGAACTCCAAGTACCACTATTGTCATTTGCAGCCACAGACCCAACTCTCTCAACCCTTCAGTTCCCATATTTTATTAAGACAACACAAAGTGATTTGTATCAAATGACTGCAGTTGCTCAAATTGTTGATCATTATGGTTGGAGAGAGGTTATTGCCATTTTTGCAGATGATGATTATGGGCGTAATGGCATATCAGCTTTAGATGATAAACTTGCTGAAAGGCGCTGTAAAATTTCCTACAAGTTGGCAATTCCCCCAGGACCGGCAGCTAATCGGAGTGCCATCATGGATATGCTTATTAAGGTTGCCTTACTGGAATCTCGCGTTATAGTTCTTCATGTGAATCCTGATTCAGGTTTCATGGTCTTTGATGCTGCAATTGGCGACATTGCCATTGTTACAAATCGAACGAAGATGGTGGATTTTACACAGCCATATGCTGCATCTGGACTTGTTGTAGTGGCCCCCTTTAAAAAATTGAACTCTGGTGCTTGGACCTTCCTTCGACCATTTACTGCACACATGTGGATAGTCACTGCTGCTTCCTTTTTTGTTGTTGGTATAGTTGTATGGATTTTGGAGCATAGGATAAATGATGAATTCAGGGGTCCACCAAAGAAGCAACTTATAACCATCTTGTGGTTATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S25

Protein Analysis

333

Amino Acids

36.74

Weight (kDa)

6.38

Isoelectric Point (pI)

31.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 42 - 205 5.3e-09 Periplasmic binding protein
ANF_receptor PF01094 49 - 243 1.2e-56 Receptor family ligand binding region
Lig_chan PF00060 290 - 332 2.2e-06 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 980
AccB7I CCANNNNNTGG 1 cut(s) 990
AccII CGCG 1 cut(s) 675
AcsI RAATTY 3 cut(s) 221, 582, 950
AcuI CTGAAG 1 cut(s) 392
AdeI CACNNNGTG 1 cut(s) 440
AfaI GTAC 1 cut(s) 363
AfiI CCNNNNNNNGG 3 cut(s) 613, 704, 990
AflIII ACRYGT 1 cut(s) 873
AgsI TTSAA 3 cut(s) 81, 263, 832
AjnI CCWGG 1 cut(s) 607
AleI CACNNNNGTG 1 cut(s) 874
AluBI AGCT 4 cut(s) 107, 266, 548, 620
AluI AGCT 4 cut(s) 107, 266, 548, 620
Alw21I GWGCWC 1 cut(s) 109
Alw26I GTCTC 1 cut(s) 275
AoxI GGCC 2 cut(s) 304, 813
ApeKI GCWGC 5 cut(s) 380, 617, 725, 791, 890
ApoI RAATTY 3 cut(s) 221, 582, 950
AspLEI GCGC 1 cut(s) 576
AspS9I GGNCC 6 cut(s) 204, 305, 611, 814, 846, 959
AvaII GGWCC 4 cut(s) 204, 611, 846, 959
BanII GRGCYC 1 cut(s) 109
Bbv12I GWGCWC 1 cut(s) 109
BbvI GCAGC 5 cut(s) 392, 629, 712, 778, 877
BccI CCATC 3 cut(s) 641, 760, 992
BciT130I CCWGG 1 cut(s) 609
BciVI GTATCC 1 cut(s) 346
BclI TGATCA 1 cut(s) 478
BcoDI GTCTC 1 cut(s) 275
BfaI CTAG 1 cut(s) 63
BfmI CTRYAG 1 cut(s) 459
BfoI RGCGCY 1 cut(s) 577
BfuI GTATCC 1 cut(s) 346
BisI GCNGC 5 cut(s) 381, 618, 726, 792, 891
BlsI GCNGC 5 cut(s) 382, 619, 727, 793, 892
Bme1390I CCNGG 1 cut(s) 609
Bme18I GGWCC 4 cut(s) 204, 611, 846, 959
BmgT120I GGNCC 6 cut(s) 204, 305, 611, 814, 846, 959
BmiI GGNNCC 3 cut(s) 205, 816, 960
BmrFI CCNGG 1 cut(s) 609
BmsI GCATC 2 cut(s) 712, 803
BsaAI YACGTR 1 cut(s) 117
BsaBI GATNNNNATC 1 cut(s) 447
BsaJI CCNNGG 3 cut(s) 24, 199, 607
Bsc4I CCNNNNNNNGG 3 cut(s) 613, 704, 990
Bse118I RCCGGY 2 cut(s) 85, 613
Bse1I ACTGG 1 cut(s) 669
Bse3DI GCAATG 2 cut(s) 342, 738
Bse8I GATNNNNATC 1 cut(s) 447
BseBI CCWGG 1 cut(s) 609
BseDI CCNNGG 3 cut(s) 24, 199, 607
BseJI GATNNNNATC 1 cut(s) 447
BseLI CCNNNNNNNGG 3 cut(s) 613, 704, 990
BseMI GCAATG 2 cut(s) 342, 738
BseNI ACTGG 1 cut(s) 669
BseRI GAGGAG 1 cut(s) 173
BseXI GCAGC 5 cut(s) 392, 629, 712, 778, 877
BsgI GTGCAG 1 cut(s) 852
Bsh1236I CGCG 1 cut(s) 675
BshFI GGCC 2 cut(s) 306, 815
BsiHKAI GWGCWC 1 cut(s) 109
BsiSI CCGG 2 cut(s) 86, 614
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 3 cut(s) 613, 704, 990
BsmAI GTCTC 1 cut(s) 275
BsmFI GGGAC 1 cut(s) 217
BsmI GAATGC 1 cut(s) 192
BsnI GGCC 2 cut(s) 306, 815
Bsp1286I GDGCHC 1 cut(s) 109
Bsp143I GATC 2 cut(s) 9, 478
Bsp19I CCATGG 1 cut(s) 199
BspANI GGCC 2 cut(s) 306, 815
BspFNI CGCG 1 cut(s) 675
BspLI GGNNCC 3 cut(s) 205, 816, 960
BspMAI CTGCAG 1 cut(s) 463
BsrDI GCAATG 2 cut(s) 342, 738
BsrFI RCCGGY 2 cut(s) 85, 613
BsrI ACTGG 1 cut(s) 669
BssAI RCCGGY 2 cut(s) 85, 613
BssECI CCNNGG 3 cut(s) 24, 199, 607
BssMI GATC 2 cut(s) 9, 478
BssT1I CCWWGG 2 cut(s) 24, 199
Bst2UI CCWGG 1 cut(s) 609
BstBAI YACGTR 1 cut(s) 117
BstDSI CCRYGG 1 cut(s) 199
BstENI CCTNNNNNAGG 1 cut(s) 702
BstFNI CGCG 1 cut(s) 675
BstH2I RGCGCY 1 cut(s) 577
BstHHI GCGC 1 cut(s) 576
BstKTI GATC 2 cut(s) 12, 481
BstMAI GTCTC 1 cut(s) 275
BstMBI GATC 2 cut(s) 9, 478
BstMWI GCNNNNNNNGC 2 cut(s) 149, 512
BstNI CCWGG 1 cut(s) 609
BstNSI RCATGY 1 cut(s) 877
BstSCI CCNGG 1 cut(s) 607
BstSFI CTRYAG 1 cut(s) 459
BstUI CGCG 1 cut(s) 675
BstV1I GCAGC 5 cut(s) 392, 629, 712, 778, 877
BsuI GTATCC 1 cut(s) 346
BsuRI GGCC 2 cut(s) 306, 815
BtgI CCRYGG 1 cut(s) 199
BtsI GCAGTG 2 cut(s) 247, 885
BtsIMutI CAGTG 2 cut(s) 247, 885
CfoI GCGC 1 cut(s) 576
Cfr10I RCCGGY 2 cut(s) 85, 613
Cfr13I GGNCC 6 cut(s) 204, 305, 611, 814, 846, 959
Csp6I GTAC 1 cut(s) 362
CspCI CAANNNNNGTGG 2 cut(s) 223, 258
CviAII CATG 6 cut(s) 200, 277, 637, 689, 712, 874
CviJI RGCY 9 cut(s) 89, 107, 266, 306, 383, 548, 620, 784, 815
CviKI_1 RGCY 9 cut(s) 89, 107, 266, 306, 383, 548, 620, 784, 815
CviQI GTAC 1 cut(s) 362
DpnI GATC 2 cut(s) 11, 480
DpnII GATC 2 cut(s) 9, 478
DraI TTTAAA 1 cut(s) 823
DraIII CACNNNGTG 1 cut(s) 440
Ecl136II GAGCTC 1 cut(s) 107
Eco130I CCWWGG 2 cut(s) 24, 199
Eco24I GRGCYC 1 cut(s) 109
Eco32I GATATC 1 cut(s) 289
Eco47I GGWCC 4 cut(s) 204, 611, 846, 959
Eco53kI GAGCTC 1 cut(s) 107
Eco57I CTGAAG 1 cut(s) 392
EcoICRI GAGCTC 1 cut(s) 107
EcoNI CCTNNNNNAGG 1 cut(s) 702
EcoRI GAATTC 1 cut(s) 950
EcoRII CCWGG 1 cut(s) 607
EcoRV GATATC 1 cut(s) 289
EcoT14I CCWWGG 2 cut(s) 24, 199
EcoT38I GRGCYC 1 cut(s) 109
ErhI CCWWGG 2 cut(s) 24, 199
FaeI CATG 6 cut(s) 203, 280, 640, 692, 715, 877
FalI AAGNNNNNCTT 2 cut(s) 644, 676
FaqI GGGAC 1 cut(s) 217
FatI CATG 6 cut(s) 199, 276, 636, 688, 711, 873
FauNDI CATATG 1 cut(s) 787
FbaI TGATCA 1 cut(s) 478
Fnu4HI GCNGC 5 cut(s) 381, 618, 726, 792, 891
FriOI GRGCYC 1 cut(s) 109
Fsp4HI GCNGC 5 cut(s) 381, 618, 726, 792, 891
FspBI CTAG 1 cut(s) 63
GlaI GCGC 1 cut(s) 575
GluI GCNGC 5 cut(s) 381, 618, 726, 792, 891
HaeII RGCGCY 1 cut(s) 577
HaeIII GGCC 2 cut(s) 306, 815
HapII CCGG 2 cut(s) 86, 614
HhaI GCGC 1 cut(s) 576
Hin1II CATG 6 cut(s) 203, 280, 640, 692, 715, 877
Hin6I GCGC 1 cut(s) 574
HinP1I GCGC 1 cut(s) 574
HincII GTYRAC 2 cut(s) 97, 178
HindII GTYRAC 2 cut(s) 97, 178
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 4 cut(s) 122, 668, 694, 701
HpaII CCGG 2 cut(s) 86, 614
Hpy166II GTNNAC 3 cut(s) 97, 178, 962
Hpy188I TCNGA 2 cut(s) 228, 627
Hpy188III TCNNGA 3 cut(s) 81, 698, 798
Hpy8I GTNNAC 3 cut(s) 97, 178, 962
HpyAV CCTTC 3 cut(s) 416, 859, 863
HpyCH4IV ACGT 1 cut(s) 116
HpyCH4V TGCA 9 cut(s) 240, 271, 347, 380, 461, 515, 728, 794, 869
HpyF10VI GCNNNNNNNGC 2 cut(s) 149, 512
HpySE526I ACGT 1 cut(s) 116
Hsp92II CATG 6 cut(s) 203, 280, 640, 692, 715, 877
HspAI GCGC 1 cut(s) 574
Ksp22I TGATCA 1 cut(s) 478
Kzo9I GATC 2 cut(s) 9, 478
LmnI GCTCC 2 cut(s) 104, 931
Lsp1109I GCAGC 5 cut(s) 392, 629, 712, 778, 877
LweI GCATC 2 cut(s) 712, 803
MaeI CTAG 1 cut(s) 63
MaeII ACGT 1 cut(s) 116
MaeIII GTNAC 2 cut(s) 748, 883
MalI GATC 2 cut(s) 11, 480
MboI GATC 2 cut(s) 9, 478
MboII GAAGA 4 cut(s) 69, 306, 677, 775
MfeI CAATTG 2 cut(s) 235, 729
MhlI GDGCHC 1 cut(s) 109
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 1 cut(s) 470
MnlI CCTC 3 cut(s) 151, 154, 490
MseI TTAA 3 cut(s) 426, 651, 822
MslI CAYNNNNRTG 2 cut(s) 641, 874
MspI CCGG 2 cut(s) 86, 614
MspR9I CCNGG 1 cut(s) 609
MunI CAATTG 2 cut(s) 235, 729
Mva1269I GAATGC 1 cut(s) 192
MvaI CCWGG 1 cut(s) 609
MvnI CGCG 1 cut(s) 675
MwoI GCNNNNNNNGC 2 cut(s) 149, 512
NcoI CCATGG 1 cut(s) 199
NdeI CATATG 1 cut(s) 787
NdeII GATC 2 cut(s) 9, 478
NlaIII CATG 6 cut(s) 203, 280, 640, 692, 715, 877
NlaIV GGNNCC 3 cut(s) 205, 816, 960
NmuCI GTSAC 1 cut(s) 883
NspI RCATGY 1 cut(s) 877
OliI CACNNNNGTG 1 cut(s) 874
PciI ACATGT 1 cut(s) 873
PctI GAATGC 1 cut(s) 192
PfeI GAWTC 3 cut(s) 668, 694, 701
PflMI CCANNNNNTGG 1 cut(s) 990
PkrI GCNGC 5 cut(s) 382, 619, 727, 793, 892
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
Ppu21I YACGTR 1 cut(s) 117
PscI ACATGT 1 cut(s) 873
PsiI TTATAA 1 cut(s) 980
Psp124BI GAGCTC 1 cut(s) 109
Psp6I CCWGG 1 cut(s) 607
PspGI CCWGG 1 cut(s) 607
PspN4I GGNNCC 3 cut(s) 205, 816, 960
PspPI GGNCC 6 cut(s) 204, 305, 611, 814, 846, 959
PsrI GAACNNNNNNTAC 2 cut(s) 345, 377
PstI CTGCAG 1 cut(s) 463
RsaI GTAC 1 cut(s) 363
RsaNI GTAC 1 cut(s) 362
RseI CAYNNNNRTG 2 cut(s) 641, 874
SacI GAGCTC 1 cut(s) 109
SaqAI TTAA 3 cut(s) 426, 651, 822
SatI GCNGC 5 cut(s) 381, 618, 726, 792, 891
Sau3AI GATC 2 cut(s) 9, 478
Sau96I GGNCC 6 cut(s) 204, 305, 611, 814, 846, 959
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 1 cut(s) 609
SduI GDGCHC 1 cut(s) 109
SfaNI GCATC 2 cut(s) 712, 803
SfcI CTRYAG 1 cut(s) 459
SinI GGWCC 4 cut(s) 204, 611, 846, 959
SmiMI CAYNNNNRTG 2 cut(s) 641, 874
SspMI CTAG 1 cut(s) 63
SstI GAGCTC 1 cut(s) 109
StyD4I CCNGG 1 cut(s) 607
StyI CCWWGG 2 cut(s) 24, 199
TaiI ACGT 1 cut(s) 119
TaqI TCGA 2 cut(s) 757, 856
TfiI GAWTC 3 cut(s) 668, 694, 701
Tru1I TTAA 3 cut(s) 426, 651, 822
Tru9I TTAA 3 cut(s) 426, 651, 822
TscAI CASTG 2 cut(s) 247, 892
TseFI GTSAC 1 cut(s) 883
TseI GCWGC 5 cut(s) 380, 617, 725, 791, 890
Tsp45I GTSAC 1 cut(s) 883
TspDTI ATGAA 5 cut(s) 265, 366, 677, 700, 963
TspRI CASTG 2 cut(s) 247, 892
Van91I CCANNNNNTGG 1 cut(s) 990
VpaK11BI GGWCC 4 cut(s) 204, 611, 846, 959
XagI CCTNNNNNAGG 1 cut(s) 702
XapI RAATTY 3 cut(s) 221, 582, 950
XceI RCATGY 1 cut(s) 877
XspI CTAG 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.