MD11G1166000.v1.1

40S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001219
Physical Location & Seq
Reverse (-)
17567711 .. 17570259
2549 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1166000.v1.1.491

Sequence Viewer

Length: 327 bp
ATGGCTCCCAAGAAGGATAAGGCTCCCCCACCGTCCTCAAAGCCTGCCAAGTCTGGCGGAGGCAAGCAGAAGAAGAAGAAGTGGAGCAAGGGAAAGCAAAAGGAGAAGGTGAACAACATGGTTTTGTTTGATCAGGGGACTTACGACAAGCTGCTTTCCGAAGTTCCGAAGTACAAGCTCATTACTCCTTCTATCTTGTCTGACAGACTAAGGATAAATGGATCCTTGGCTCGCCGTGCAATTAAGGATTTGATGGCAAGGGGTTCCATTAGGCTGATAGCTGCTCATGCTAGCCAGCAGATTTACACCAGAGCCACCAATACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S25

Protein Analysis

109

Amino Acids

11.98

Weight (kDa)

10.59

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S25 PF03297 7 - 105 2.8e-41 S25 ribosomal protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 57
AclWI GGATC 2 cut(s) 216, 229
AfaI GTAC 1 cut(s) 173
AluBI AGCT 3 cut(s) 151, 178, 281
AluI AGCT 3 cut(s) 151, 178, 281
AlwI GGATC 2 cut(s) 216, 229
ApeKI GCWGC 2 cut(s) 151, 281
AsuHPI GGTGA 1 cut(s) 121
AsuNHI GCTAGC 1 cut(s) 290
BamHI GGATCC 1 cut(s) 221
BbvI GCAGC 2 cut(s) 138, 268
BccI CCATC 1 cut(s) 247
BceAI ACGGC 1 cut(s) 219
BclI TGATCA 1 cut(s) 130
BfaI CTAG 1 cut(s) 291
BisI GCNGC 2 cut(s) 152, 282
BlsI GCNGC 2 cut(s) 153, 283
BmiI GGNNCC 4 cut(s) 6, 24, 223, 265
BmtI GCTAGC 1 cut(s) 294
BsaJI CCNNGG 1 cut(s) 225
BseDI CCNNGG 1 cut(s) 225
BseXI GCAGC 2 cut(s) 138, 268
BslFI GGGAC 1 cut(s) 151
BsmFI GGGAC 1 cut(s) 151
Bsp143I GATC 2 cut(s) 130, 221
BspACI CCGC 1 cut(s) 57
BspLI GGNNCC 4 cut(s) 6, 24, 223, 265
BspOI GCTAGC 1 cut(s) 294
BspPI GGATC 2 cut(s) 216, 229
BssECI CCNNGG 1 cut(s) 225
BssMI GATC 2 cut(s) 130, 221
BssT1I CCWWGG 1 cut(s) 225
Bst4CI ACNGT 1 cut(s) 33
BstC8I GCNNGC 5 cut(s) 45, 65, 232, 292, 296
BstDEI CTNAG 1 cut(s) 209
BstKTI GATC 2 cut(s) 133, 224
BstMBI GATC 2 cut(s) 130, 221
BstMWI GCNNNNNNNGC 2 cut(s) 236, 287
BstV1I GCAGC 2 cut(s) 138, 268
BstX2I RGATCY 1 cut(s) 221
BstYI RGATCY 1 cut(s) 221
Cac8I GCNNGC 5 cut(s) 45, 65, 232, 292, 296
Csp6I GTAC 1 cut(s) 172
CviAII CATG 2 cut(s) 118, 287
CviQI GTAC 1 cut(s) 172
DdeI CTNAG 1 cut(s) 209
DpnI GATC 2 cut(s) 132, 223
DpnII GATC 2 cut(s) 130, 221
EciI GGCGGA 1 cut(s) 72
Eco130I CCWWGG 1 cut(s) 225
EcoT14I CCWWGG 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 225
FaeI CATG 2 cut(s) 121, 290
FaiI YATR 2 cut(s) 119, 288
FaqI GGGAC 1 cut(s) 151
FatI CATG 2 cut(s) 117, 286
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 2 cut(s) 152, 282
Fsp4HI GCNGC 2 cut(s) 152, 282
FspBI CTAG 1 cut(s) 291
GluI GCNGC 2 cut(s) 152, 282
Hin1II CATG 2 cut(s) 121, 290
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 1 cut(s) 112
Hpy188I TCNGA 3 cut(s) 160, 168, 202
Hpy8I GTNNAC 1 cut(s) 112
HpyAV CCTTC 3 cut(s) 7, 100, 198
HpyCH4III ACNGT 1 cut(s) 33
HpyCH4V TGCA 1 cut(s) 239
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 287
HpyF3I CTNAG 1 cut(s) 209
Hsp92II CATG 2 cut(s) 121, 290
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 2 cut(s) 130, 221
LmnI GCTCC 3 cut(s) 10, 28, 84
LpnPI CCDG 5 cut(s) 39, 57, 119, 308, 322
Lsp1109I GCAGC 2 cut(s) 138, 268
MaeI CTAG 1 cut(s) 291
MalI GATC 2 cut(s) 132, 223
MboI GATC 2 cut(s) 130, 221
MboII GAAGA 3 cut(s) 82, 85, 88
MflI RGATCY 1 cut(s) 221
MluCI AATT 1 cut(s) 240
MnlI CCTC 2 cut(s) 46, 53
MseI TTAA 2 cut(s) 243, 325
MwoI GCNNNNNNNGC 2 cut(s) 236, 287
NdeII GATC 2 cut(s) 130, 221
NheI GCTAGC 1 cut(s) 290
NlaIII CATG 2 cut(s) 121, 290
NlaIV GGNNCC 4 cut(s) 6, 24, 223, 265
PkrI GCNGC 2 cut(s) 153, 283
PspN4I GGNNCC 4 cut(s) 6, 24, 223, 265
PsuI RGATCY 1 cut(s) 221
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
SaqAI TTAA 2 cut(s) 243, 325
SatI GCNGC 2 cut(s) 152, 282
Sau3AI GATC 2 cut(s) 130, 221
SetI ASST 4 cut(s) 111, 153, 180, 283
Sse9I AATT 1 cut(s) 240
SsiI CCGC 1 cut(s) 57
SspMI CTAG 1 cut(s) 291
StyI CCWWGG 1 cut(s) 225
TaaI ACNGT 1 cut(s) 33
TasI AATT 1 cut(s) 240
TatI WGTACW 1 cut(s) 171
Tru1I TTAA 2 cut(s) 243, 325
Tru9I TTAA 2 cut(s) 243, 325
TseI GCWGC 2 cut(s) 151, 281
XspI CTAG 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.