MD03G1183800.v1.1

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002844
Physical Location & Seq
Forward (+)
25091646 .. 25096151
4506 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1183800.v1.1.491

Sequence Viewer

Length: 1062 bp
ATGGGATCGAATGAGAGGGGATCCTTACTTTCAGGACTAACAGATCCTTTGTCTCAAACAAATAAAAACAATGCTCCAAAGGGCAGGAGAATTCGTCGTTGTAGAAGTGCTCCTGTTTCAGATTATGTAGCCTTAGAGACAGGCTTACCCTCAGGTCCACAAACCGATCAGTCCTTTTTCGGAAATATACACCCGAGTTTTCGCATTGTAGCTGCATTCTTGACTGTCTACCTAGGCTTAGGAACTGTATGTTTCTACCTAGTCAGGAACCAGCTCAAGGGAGAGAAGACAAATGGAGTTCTTGATGCTGTTTATTTCTGTATTGTGACAATGACCACTGTCGGATATGGTGACCTTGTGCCAAACAGCGTTCTTTCAAAACTAGTGGCTTGTGCTTTCGTCTTCTCAGGAATGGCTCTCGTCGTAATGATCTTGGGCAAAGCAGCTGACTATTTGGTAGAGAAGCAGGAATTATTGCTCGTTAAAGCCCTACATACGAGTAAAAAAGTTGGGCATATTGAAATTCTTAAAGATATCGAGACAAACAGTCCGAGGTACAAATGTATTGTGGTCTTTATCCTTCTTTTGCTACTCATAATTGGTGGCACAATCTTCCTAGCTACTGTTGAGAAATTGAGCCTTGTGGATTCATTCTATTGCGTTTGTTGTACCATCACAACCCTGGGTTATGGAGATAAGAGCTTTTCGTCTCAAGCAGGGCGTGCTTTTGCAGTATTCTGGATATTGACAGGTACCATTTGTTTAGCTCAGTTTTTCCTCTACGTTGCCGAGCTAAACGCCCAAAGCAGACAACGGGCGTTAGTAAAGTTGGTTCTCACTCGTAGGGTGACCAATGTAGATTTAGAGGCAGCTGATCTGGATGACGATGGCGTTGTTGGGGCTGCTGAATTTGTCATATACAAGCTCAAAGAGATGGGGAAGATTAGCCAGGAAGATGTTAGACTTGTAATGGAGGAGTTTGAAGATCTTGATGTTGATCAGTCAGGAACGTTGTCAACATCCGATATAATACTTGCGCAACAACCTCAAATCAAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0003824 GO:0004788 GO:0005215 GO:0005216 GO:0005242 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005887 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006873 GO:0006875 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009108 GO:0009229 GO:0009705 GO:0009987 GO:0010029 GO:0010119 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015276 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016043 GO:0016740 GO:0016772 GO:0016778 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019725 GO:0022607 GO:0022803 GO:0022832 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022840 GO:0022841 GO:0022842 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0030004 GO:0030007 GO:0030322 GO:0031004 GO:0031090 GO:0031224 GO:0031226 GO:0032991 GO:0034220 GO:0034641 GO:0042357 GO:0042391 GO:0042592 GO:0042723 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046483 GO:0046873 GO:0048580 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050801 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051239 GO:0051259 GO:0051260 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0055085 GO:0065003 GO:0065007 GO:0065008 GO:0071704 GO:0071804 GO:0071805 GO:0071840 GO:0071944 GO:0072527 GO:0072528 GO:0090407 GO:0090533 GO:0098533 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0099094 GO:1900140 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902494 GO:1902495 GO:1904949 GO:1990351 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

354

Amino Acids

38.68

Weight (kDa)

6.82

Isoelectric Point (pI)

34.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans_2 PF07885 73 - 153 4.8e-17 Ion channel
Ion_trans PF00520 80 - 156 3.1e-06 Ion transport protein
Ion_trans_2 PF07885 193 - 265 6.2e-16 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1038
Acc65I GGTACC 1 cut(s) 752
AccB1I GGYRCC 1 cut(s) 752
AccI GTMKAC 1 cut(s) 228
AclI AACGTT 1 cut(s) 1010
AclWI GGATC 4 cut(s) 13, 15, 28, 38
AcsI RAATTY 3 cut(s) 90, 522, 908
AfaI GTAC 3 cut(s) 557, 670, 754
AfiI CCNNNNNNNGG 1 cut(s) 277
AgsI TTSAA 3 cut(s) 378, 521, 983
AhdI GACNNNNNGTC 1 cut(s) 546
AhlI ACTAGT 1 cut(s) 382
AjnI CCWGG 2 cut(s) 681, 948
AluBI AGCT 9 cut(s) 212, 274, 446, 620, 702, 767, 793, 872, 925
AluI AGCT 9 cut(s) 212, 274, 446, 620, 702, 767, 793, 872, 925
Alw21I GWGCWC 1 cut(s) 112
Alw26I GTCTC 4 cut(s) 57, 131, 533, 714
AlwI GGATC 4 cut(s) 13, 15, 28, 38
Ama87I CYCGRG 1 cut(s) 193
ApeKI GCWGC 4 cut(s) 212, 443, 869, 902
ApoI RAATTY 3 cut(s) 90, 522, 908
Asp718I GGTACC 1 cut(s) 752
AspA2I CCTAGG 1 cut(s) 232
AspLEI GCGC 1 cut(s) 1039
AspS9I GGNCC 1 cut(s) 155
AsuHPI GGTGA 2 cut(s) 362, 859
AvaI CYCGRG 1 cut(s) 193
AvaII GGWCC 1 cut(s) 155
AvrII CCTAGG 1 cut(s) 232
AxyI CCTNAGG 1 cut(s) 151
BaeI ACNNNNGTAYC 2 cut(s) 547, 580
BamHI GGATCC 1 cut(s) 20
BanI GGYRCC 1 cut(s) 752
BbsI GAAGAC 2 cut(s) 293, 394
Bbv12I GWGCWC 1 cut(s) 112
BbvI GCAGC 4 cut(s) 199, 455, 881, 889
BccI CCATC 3 cut(s) 680, 881, 928
BciT130I CCWGG 2 cut(s) 683, 950
BclI TGATCA 1 cut(s) 997
BcoDI GTCTC 4 cut(s) 57, 131, 533, 714
BcuI ACTAGT 1 cut(s) 382
BfaI CTAG 4 cut(s) 233, 260, 383, 617
BglII AGATCT 1 cut(s) 985
BisI GCNGC 4 cut(s) 213, 444, 870, 903
BlnI CCTAGG 1 cut(s) 232
BlsI GCNGC 4 cut(s) 214, 445, 871, 904
Bme1390I CCNGG 2 cut(s) 683, 950
Bme18I GGWCC 1 cut(s) 155
BmeRI GACNNNNNGTC 1 cut(s) 546
BmeT110I CYCGRG 1 cut(s) 193
BmgT120I GGNCC 1 cut(s) 155
BmiI GGNNCC 3 cut(s) 22, 269, 754
BmrFI CCNGG 2 cut(s) 683, 950
BmsI GCATC 1 cut(s) 295
BoxI GACNNNNGTC 1 cut(s) 338
BpiI GAAGAC 2 cut(s) 293, 394
Bpu10I CCTNAGC 1 cut(s) 238
BpuEI CTTGAG 2 cut(s) 260, 696
BsaBI GATNNNNATC 1 cut(s) 996
BsaJI CCNNGG 4 cut(s) 232, 551, 681, 682
BsaXI ACNNNNNCTCC 2 cut(s) 79, 109
Bsc4I CCNNNNNNNGG 1 cut(s) 277
Bse21I CCTNAGG 1 cut(s) 151
Bse8I GATNNNNATC 1 cut(s) 996
BseBI CCWGG 2 cut(s) 683, 950
BseDI CCNNGG 4 cut(s) 232, 551, 681, 682
BseGI GGATG 2 cut(s) 886, 1019
BseJI GATNNNNATC 1 cut(s) 996
BseLI CCNNNNNNNGG 1 cut(s) 277
BseMII CTCAG 3 cut(s) 165, 420, 782
BseRI GAGGAG 1 cut(s) 989
BseXI GCAGC 4 cut(s) 199, 455, 881, 889
BshNI GGYRCC 1 cut(s) 752
BsiHKAI GWGCWC 1 cut(s) 112
BsiHKCI CYCGRG 1 cut(s) 193
BslI CCNNNNNNNGG 1 cut(s) 277
BsmAI GTCTC 4 cut(s) 57, 131, 533, 714
BsmBI CGTCTC 1 cut(s) 714
BsmI GAATGC 1 cut(s) 215
BsoBI CYCGRG 1 cut(s) 193
Bsp1286I GDGCHC 1 cut(s) 112
Bsp143I GATC 8 cut(s) 5, 20, 43, 166, 429, 874, 985, 997
BspCNI CTCAG 3 cut(s) 164, 419, 781
BspLI GGNNCC 3 cut(s) 22, 269, 754
BspPI GGATC 4 cut(s) 13, 15, 28, 38
BspT107I GGYRCC 1 cut(s) 752
BssECI CCNNGG 4 cut(s) 232, 551, 681, 682
BssMI GATC 8 cut(s) 5, 20, 43, 166, 429, 874, 985, 997
BssT1I CCWWGG 1 cut(s) 232
Bst2UI CCWGG 2 cut(s) 683, 950
Bst4CI ACNGT 5 cut(s) 226, 247, 340, 548, 625
BstAPI GCANNNNNTGC 1 cut(s) 722
BstC8I GCNNGC 1 cut(s) 723
BstDEI CTNAG 5 cut(s) 133, 151, 238, 406, 768
BstEII GGTNACC 2 cut(s) 350, 847
BstF5I GGATG 2 cut(s) 886, 1019
BstHHI GCGC 1 cut(s) 1039
BstKTI GATC 8 cut(s) 8, 23, 46, 169, 432, 877, 988, 1000
BstMAI GTCTC 4 cut(s) 57, 131, 533, 714
BstMBI GATC 8 cut(s) 5, 20, 43, 166, 429, 874, 985, 997
BstMWI GCNNNNNNNGC 1 cut(s) 722
BstNI CCWGG 2 cut(s) 683, 950
BstPAI GACNNNNGTC 1 cut(s) 338
BstPI GGTNACC 2 cut(s) 350, 847
BstSCI CCNGG 2 cut(s) 681, 948
BstV1I GCAGC 4 cut(s) 199, 455, 881, 889
BstV2I GAAGAC 2 cut(s) 293, 394
BstX2I RGATCY 3 cut(s) 20, 43, 985
BstYI RGATCY 3 cut(s) 20, 43, 985
Bsu36I CCTNAGG 1 cut(s) 151
BtsCI GGATG 2 cut(s) 886, 1019
BtsIMutI CAGTG 1 cut(s) 336
Cac8I GCNNGC 1 cut(s) 723
CfoI GCGC 1 cut(s) 1039
Cfr13I GGNCC 1 cut(s) 155
Csp6I GTAC 3 cut(s) 556, 669, 753
CspCI CAANNNNNGTGG 2 cut(s) 366, 401
CviQI GTAC 3 cut(s) 556, 669, 753
DdeI CTNAG 5 cut(s) 133, 151, 238, 406, 768
DpnI GATC 8 cut(s) 7, 22, 45, 168, 431, 876, 987, 999
DpnII GATC 8 cut(s) 5, 20, 43, 166, 429, 874, 985, 997
DriI GACNNNNNGTC 1 cut(s) 546
Eam1105I GACNNNNNGTC 1 cut(s) 546
Eco130I CCWWGG 1 cut(s) 232
Eco32I GATATC 1 cut(s) 535
Eco47I GGWCC 1 cut(s) 155
Eco81I CCTNAGG 1 cut(s) 151
Eco88I CYCGRG 1 cut(s) 193
Eco91I GGTNACC 2 cut(s) 350, 847
EcoO65I GGTNACC 2 cut(s) 350, 847
EcoRI GAATTC 1 cut(s) 90
EcoRII CCWGG 2 cut(s) 681, 948
EcoRV GATATC 1 cut(s) 535
EcoT14I CCWWGG 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 232
Esp3I CGTCTC 1 cut(s) 714
FbaI TGATCA 1 cut(s) 997
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 4 cut(s) 213, 444, 870, 903
FokI GGATG 2 cut(s) 893, 1006
Fsp4HI GCNGC 4 cut(s) 213, 444, 870, 903
FspBI CTAG 4 cut(s) 233, 260, 383, 617
FspI TGCGCA 1 cut(s) 1038
GlaI GCGC 1 cut(s) 1038
GluI GCNGC 4 cut(s) 213, 444, 870, 903
HhaI GCGC 1 cut(s) 1039
Hin6I GCGC 1 cut(s) 1037
HinP1I GCGC 1 cut(s) 1037
HincII GTYRAC 1 cut(s) 1017
HindII GTYRAC 1 cut(s) 1017
HinfI GANTC 1 cut(s) 647
HphI GGTGA 2 cut(s) 362, 859
Hpy166II GTNNAC 3 cut(s) 158, 229, 1017
Hpy188I TCNGA 5 cut(s) 121, 182, 344, 552, 1024
Hpy8I GTNNAC 3 cut(s) 158, 229, 1017
Hpy99I CGWCG 2 cut(s) 99, 425
HpyAV CCTTC 1 cut(s) 590
HpyCH4III ACNGT 5 cut(s) 226, 247, 340, 548, 625
HpyCH4IV ACGT 2 cut(s) 783, 1010
HpyCH4V TGCA 2 cut(s) 215, 731
HpyF10VI GCNNNNNNNGC 1 cut(s) 722
HpyF3I CTNAG 5 cut(s) 133, 151, 238, 406, 768
HpySE526I ACGT 2 cut(s) 783, 1010
HspAI GCGC 1 cut(s) 1037
KpnI GGTACC 1 cut(s) 756
Ksp22I TGATCA 1 cut(s) 997
Kzo9I GATC 8 cut(s) 5, 20, 43, 166, 429, 874, 985, 997
LmnI GCTCC 2 cut(s) 79, 115
Lsp1109I GCAGC 4 cut(s) 199, 455, 881, 889
LweI GCATC 1 cut(s) 295
MaeI CTAG 4 cut(s) 233, 260, 383, 617
MaeII ACGT 2 cut(s) 783, 1010
MaeIII GTNAC 3 cut(s) 325, 350, 847
MalI GATC 8 cut(s) 7, 22, 45, 168, 431, 876, 987, 999
MboI GATC 8 cut(s) 5, 20, 43, 166, 429, 874, 985, 997
MboII GAAGA 6 cut(s) 298, 394, 604, 952, 965, 995
MflI RGATCY 3 cut(s) 20, 43, 985
MhlI GDGCHC 1 cut(s) 112
MluCI AATT 6 cut(s) 90, 470, 522, 597, 632, 908
MmeI TCCRAC 1 cut(s) 322
MnlI CCTC 7 cut(s) 9, 160, 546, 788, 859, 967, 1056
MseI TTAA 2 cut(s) 483, 528
MspA1I CMGCKG 2 cut(s) 446, 872
MspR9I CCNGG 2 cut(s) 683, 950
Mva1269I GAATGC 1 cut(s) 215
MvaI CCWGG 2 cut(s) 683, 950
MwoI GCNNNNNNNGC 1 cut(s) 722
NdeII GATC 8 cut(s) 5, 20, 43, 166, 429, 874, 985, 997
NlaIV GGNNCC 3 cut(s) 22, 269, 754
NmeAIII GCCGAG 1 cut(s) 814
NmuCI GTSAC 3 cut(s) 325, 350, 847
NsbI TGCGCA 1 cut(s) 1038
PasI CCCWGGG 1 cut(s) 682
PctI GAATGC 1 cut(s) 215
PfeI GAWTC 1 cut(s) 647
PkrI GCNGC 4 cut(s) 214, 445, 871, 904
PshAI GACNNNNGTC 1 cut(s) 338
Psp1406I AACGTT 1 cut(s) 1010
Psp6I CCWGG 2 cut(s) 681, 948
PspEI GGTNACC 2 cut(s) 350, 847
PspGI CCWGG 2 cut(s) 681, 948
PspN4I GGNNCC 3 cut(s) 22, 269, 754
PspPI GGNCC 1 cut(s) 155
PsrI GAACNNNNNNTAC 2 cut(s) 816, 848
PsuI RGATCY 3 cut(s) 20, 43, 985
PvuII CAGCTG 2 cut(s) 446, 872
RsaI GTAC 3 cut(s) 557, 670, 754
RsaNI GTAC 3 cut(s) 556, 669, 753
SaqAI TTAA 2 cut(s) 483, 528
SatI GCNGC 4 cut(s) 213, 444, 870, 903
Sau3AI GATC 8 cut(s) 5, 20, 43, 166, 429, 874, 985, 997
Sau96I GGNCC 1 cut(s) 155
ScrFI CCNGG 2 cut(s) 683, 950
SduI GDGCHC 1 cut(s) 112
SfaNI GCATC 1 cut(s) 295
SinI GGWCC 1 cut(s) 155
SmlI CTYRAG 2 cut(s) 275, 711
SmoI CTYRAG 2 cut(s) 275, 711
SpeI ACTAGT 1 cut(s) 382
Sse9I AATT 6 cut(s) 90, 470, 522, 597, 632, 908
SspMI CTAG 4 cut(s) 233, 260, 383, 617
StyD4I CCNGG 2 cut(s) 681, 948
StyI CCWWGG 1 cut(s) 232
TaaI ACNGT 5 cut(s) 226, 247, 340, 548, 625
TaiI ACGT 2 cut(s) 786, 1013
TaqI TCGA 2 cut(s) 8, 537
TasI AATT 6 cut(s) 90, 470, 522, 597, 632, 908
TfiI GAWTC 1 cut(s) 647
Tru1I TTAA 2 cut(s) 483, 528
Tru9I TTAA 2 cut(s) 483, 528
TscAI CASTG 1 cut(s) 343
TseFI GTSAC 3 cut(s) 325, 350, 847
TseI GCWGC 4 cut(s) 212, 443, 869, 902
Tsp45I GTSAC 3 cut(s) 325, 350, 847
TspDTI ATGAA 1 cut(s) 639
TspRI CASTG 1 cut(s) 343
VpaK11BI GGWCC 1 cut(s) 155
XapI RAATTY 3 cut(s) 90, 522, 908
XcmI CCANNNNNNNNNTGG 1 cut(s) 679
XmaJI CCTAGG 1 cut(s) 232
XmiI GTMKAC 1 cut(s) 228
XspI CTAG 4 cut(s) 233, 260, 383, 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.