pycom03g13730

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0002844
Physical Location & Seq
Forward (+)
14836659 .. 14837798
1140 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g13730.1

Sequence Viewer

Length: 1023 bp
ATGGATCCTTTGTCTCGAACAAATAAAAACAATGCTCCAAAGGGTAGGAGAATTCGTCGTTGTAGAAGTGCTCCTGTTTCAGATTATGTAGCCTTAGAGACAGGCTTCCCCTCAGGTCCACGTACCGATCAGTCCTTTTTTGGAAATATACACCCGAGTTTTCGCATTGTAGCTGCATTCTTGACTGTCTACTTAGGCTTAGGAACTGTATGTTTCTACCTAGTCAGGAACCAGCTCAAGGGAGAGAAGACAAATGGAGTTGTTGATGCTGTTTATTTCTGTATTGTGACAATGACCACTGTCGGATATGGAGACCTTGTGCCAAACAGCGTTCTTTCAAAACTACTGGCTTGTGCTTTCGTCTTCTCAGGAATGGCTCTCATCGGGATGATCTTGAGCAAAGCAGCTGACTATTTGGTAGAGAAGCAGGAATTATTGCTCGTTAAAGCCCTACATACGAGTAAAAAAGTCGGGCATATTGAAATTCTTAAAGATATCGAGACAAACAGTCTGAGGTACAAATGTATTGTGGTCTTTATCCTTCTTTTGCTACTCATAATTGGTGGCACAATCTTCCTAGCTACTGTTGAGAAATTGAGCCTTGTGGATTCATTCTATTGCGTTTGTTGTACCATCACAACCATGGGTTATGGAGATAAGAGCTTTTCAACTCAAGCAGGGCGTGCTTTTGCAGTATTCTGGATATTGACAGGTACCATTTGTTTAGCTCAGTTTTTCCTCTACGTTGCCGAGCTAAATGCCCAGAGCAGACAACGGGCTTTAGTAAAGTTGATTCTCACTCGTAGGATAACCAATGTAGATTTAGAGGCAGCTGATCTGGATGACGATGGCGTTGTTGGGGCTGCTGAATTTGTCATATACAAGCTCAAGGAGATGGGGAAGATTAGCCAGGAAGATGTTAGACTTGTAATGGAGGAGTTTGAAGATCTTGATGTTGATCAGTCAGGAACGTTGTCAACATCAGATATAATGCTCGCGCAACAACCTCAAATCGAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0003824 GO:0004788 GO:0005215 GO:0005216 GO:0005242 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005887 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006873 GO:0006875 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009108 GO:0009229 GO:0009705 GO:0009987 GO:0010029 GO:0010119 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015276 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016043 GO:0016740 GO:0016772 GO:0016778 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019725 GO:0022607 GO:0022803 GO:0022832 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022840 GO:0022841 GO:0022842 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0030004 GO:0030007 GO:0030322 GO:0031004 GO:0031090 GO:0031224 GO:0031226 GO:0032991 GO:0034220 GO:0034641 GO:0042357 GO:0042391 GO:0042592 GO:0042723 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046483 GO:0046873 GO:0048580 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050801 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051239 GO:0051259 GO:0051260 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0055085 GO:0065003 GO:0065007 GO:0065008 GO:0071704 GO:0071804 GO:0071805 GO:0071840 GO:0071944 GO:0072527 GO:0072528 GO:0090407 GO:0090533 GO:0098533 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0099094 GO:1900140 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902494 GO:1902495 GO:1904949 GO:1990351 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

341

Amino Acids

37.6

Weight (kDa)

6.82

Isoelectric Point (pI)

34.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans_2 PF07885 60 - 140 4.4e-17 Ion channel
Ion_trans_2 PF07885 180 - 252 5.4e-16 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 713
AccB1I GGYRCC 1 cut(s) 713
AccI GTMKAC 1 cut(s) 189
AccII CGCG 1 cut(s) 998
AclI AACGTT 1 cut(s) 971
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 3 cut(s) 51, 483, 869
AfaI GTAC 4 cut(s) 124, 518, 631, 715
AfiI CCNNNNNNNGG 1 cut(s) 238
AgsI TTSAA 4 cut(s) 339, 482, 669, 944
AhdI GACNNNNNGTC 1 cut(s) 507
AjnI CCWGG 1 cut(s) 909
AluBI AGCT 9 cut(s) 173, 235, 407, 581, 663, 728, 754, 833, 886
AluI AGCT 9 cut(s) 173, 235, 407, 581, 663, 728, 754, 833, 886
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 4 cut(s) 18, 92, 306, 494
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 154
ApeKI GCWGC 4 cut(s) 173, 404, 830, 863
ApoI RAATTY 3 cut(s) 51, 483, 869
Asp718I GGTACC 1 cut(s) 713
AspLEI GCGC 1 cut(s) 1000
AspS9I GGNCC 1 cut(s) 116
AvaI CYCGRG 1 cut(s) 154
AvaII GGWCC 1 cut(s) 116
AxyI CCTNAGG 1 cut(s) 112
BaeI ACNNNNGTAYC 2 cut(s) 508, 541
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 713
BbsI GAAGAC 2 cut(s) 254, 355
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 4 cut(s) 160, 416, 842, 850
BccI CCATC 3 cut(s) 641, 842, 889
BcgI CGANNNNNNTGC 2 cut(s) 740, 774
BciT130I CCWGG 1 cut(s) 911
BclI TGATCA 1 cut(s) 958
BcoDI GTCTC 4 cut(s) 18, 92, 306, 494
BfaI CTAG 2 cut(s) 221, 578
BglII AGATCT 1 cut(s) 946
BisI GCNGC 4 cut(s) 174, 405, 831, 864
BlsI GCNGC 4 cut(s) 175, 406, 832, 865
Bme1390I CCNGG 1 cut(s) 911
Bme18I GGWCC 1 cut(s) 116
BmeRI GACNNNNNGTC 1 cut(s) 507
BmeT110I CYCGRG 1 cut(s) 154
BmgT120I GGNCC 1 cut(s) 116
BmiI GGNNCC 3 cut(s) 6, 230, 715
BmrFI CCNGG 1 cut(s) 911
BmsI GCATC 1 cut(s) 256
BoxI GACNNNNGTC 1 cut(s) 299
BpiI GAAGAC 2 cut(s) 254, 355
Bpu10I CCTNAGC 1 cut(s) 199
BpuEI CTTGAG 4 cut(s) 221, 415, 657, 872
BsaAI YACGTR 1 cut(s) 122
BsaBI GATNNNNATC 1 cut(s) 957
BsaI GGTCTC 1 cut(s) 306
BsaJI CCNNGG 1 cut(s) 642
BsaXI ACNNNNNCTCC 4 cut(s) 40, 70, 303, 333
Bsc4I CCNNNNNNNGG 1 cut(s) 238
Bse1I ACTGG 1 cut(s) 351
Bse21I CCTNAGG 1 cut(s) 112
Bse8I GATNNNNATC 1 cut(s) 957
BseBI CCWGG 1 cut(s) 911
BseDI CCNNGG 1 cut(s) 642
BseGI GGATG 2 cut(s) 393, 847
BseJI GATNNNNATC 1 cut(s) 957
BseLI CCNNNNNNNGG 1 cut(s) 238
BseMII CTCAG 4 cut(s) 126, 381, 503, 743
BseNI ACTGG 1 cut(s) 351
BseRI GAGGAG 1 cut(s) 950
BseXI GCAGC 4 cut(s) 160, 416, 842, 850
Bsh1236I CGCG 1 cut(s) 998
BshNI GGYRCC 1 cut(s) 713
BsiHKAI GWGCWC 1 cut(s) 73
BsiHKCI CYCGRG 1 cut(s) 154
BslI CCNNNNNNNGG 1 cut(s) 238
BsmAI GTCTC 4 cut(s) 18, 92, 306, 494
BsmI GAATGC 1 cut(s) 176
Bso31I GGTCTC 1 cut(s) 306
BsoBI CYCGRG 1 cut(s) 154
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 6 cut(s) 4, 127, 390, 835, 946, 958
Bsp19I CCATGG 1 cut(s) 642
BspCNI CTCAG 4 cut(s) 125, 380, 504, 742
BspFNI CGCG 1 cut(s) 998
BspLI GGNNCC 3 cut(s) 6, 230, 715
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 713
BspTNI GGTCTC 1 cut(s) 306
BsrI ACTGG 1 cut(s) 351
BssECI CCNNGG 1 cut(s) 642
BssMI GATC 6 cut(s) 4, 127, 390, 835, 946, 958
BssT1I CCWWGG 1 cut(s) 642
Bst2UI CCWGG 1 cut(s) 911
Bst4CI ACNGT 5 cut(s) 187, 208, 301, 509, 586
BstAPI GCANNNNNTGC 1 cut(s) 683
BstBAI YACGTR 1 cut(s) 122
BstC8I GCNNGC 2 cut(s) 684, 996
BstDEI CTNAG 7 cut(s) 94, 112, 193, 199, 367, 512, 729
BstDSI CCRYGG 1 cut(s) 642
BstF5I GGATG 2 cut(s) 393, 847
BstFNI CGCG 1 cut(s) 998
BstHHI GCGC 1 cut(s) 1000
BstKTI GATC 6 cut(s) 7, 130, 393, 838, 949, 961
BstMAI GTCTC 4 cut(s) 18, 92, 306, 494
BstMBI GATC 6 cut(s) 4, 127, 390, 835, 946, 958
BstMWI GCNNNNNNNGC 1 cut(s) 683
BstNI CCWGG 1 cut(s) 911
BstPAI GACNNNNGTC 1 cut(s) 299
BstSCI CCNGG 1 cut(s) 909
BstUI CGCG 1 cut(s) 998
BstV1I GCAGC 4 cut(s) 160, 416, 842, 850
BstV2I GAAGAC 2 cut(s) 254, 355
BstX2I RGATCY 2 cut(s) 4, 946
BstYI RGATCY 2 cut(s) 4, 946
Bsu36I CCTNAGG 1 cut(s) 112
BtgI CCRYGG 1 cut(s) 642
BtsCI GGATG 2 cut(s) 393, 847
BtsIMutI CAGTG 1 cut(s) 297
Cac8I GCNNGC 2 cut(s) 684, 996
CfoI GCGC 1 cut(s) 1000
Cfr13I GGNCC 1 cut(s) 116
Csp6I GTAC 4 cut(s) 123, 517, 630, 714
CviAII CATG 1 cut(s) 643
CviQI GTAC 4 cut(s) 123, 517, 630, 714
DdeI CTNAG 7 cut(s) 94, 112, 193, 199, 367, 512, 729
DpnI GATC 6 cut(s) 6, 129, 392, 837, 948, 960
DpnII GATC 6 cut(s) 4, 127, 390, 835, 946, 958
DriI GACNNNNNGTC 1 cut(s) 507
Eam1105I GACNNNNNGTC 1 cut(s) 507
Eco130I CCWWGG 1 cut(s) 642
Eco31I GGTCTC 1 cut(s) 306
Eco32I GATATC 1 cut(s) 496
Eco47I GGWCC 1 cut(s) 116
Eco81I CCTNAGG 1 cut(s) 112
Eco88I CYCGRG 1 cut(s) 154
EcoRI GAATTC 1 cut(s) 51
EcoRII CCWGG 1 cut(s) 909
EcoRV GATATC 1 cut(s) 496
EcoT14I CCWWGG 1 cut(s) 642
ErhI CCWWGG 1 cut(s) 642
FaeI CATG 1 cut(s) 646
FatI CATG 1 cut(s) 642
FbaI TGATCA 1 cut(s) 958
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 4 cut(s) 174, 405, 831, 864
FokI GGATG 2 cut(s) 400, 854
Fsp4HI GCNGC 4 cut(s) 174, 405, 831, 864
FspBI CTAG 2 cut(s) 221, 578
GlaI GCGC 1 cut(s) 999
GluI GCNGC 4 cut(s) 174, 405, 831, 864
HhaI GCGC 1 cut(s) 1000
Hin1II CATG 1 cut(s) 646
Hin6I GCGC 1 cut(s) 998
HinP1I GCGC 1 cut(s) 998
HincII GTYRAC 1 cut(s) 978
HindII GTYRAC 1 cut(s) 978
HinfI GANTC 2 cut(s) 608, 793
Hpy166II GTNNAC 3 cut(s) 119, 190, 978
Hpy188I TCNGA 4 cut(s) 82, 305, 513, 985
Hpy8I GTNNAC 3 cut(s) 119, 190, 978
Hpy99I CGWCG 1 cut(s) 60
HpyAV CCTTC 1 cut(s) 551
HpyCH4III ACNGT 5 cut(s) 187, 208, 301, 509, 586
HpyCH4IV ACGT 3 cut(s) 121, 744, 971
HpyCH4V TGCA 2 cut(s) 176, 692
HpyF10VI GCNNNNNNNGC 1 cut(s) 683
HpyF3I CTNAG 7 cut(s) 94, 112, 193, 199, 367, 512, 729
HpySE526I ACGT 3 cut(s) 121, 744, 971
Hsp92II CATG 1 cut(s) 646
HspAI GCGC 1 cut(s) 998
KpnI GGTACC 1 cut(s) 717
Ksp22I TGATCA 1 cut(s) 958
Kzo9I GATC 6 cut(s) 4, 127, 390, 835, 946, 958
LmnI GCTCC 2 cut(s) 40, 76
Lsp1109I GCAGC 4 cut(s) 160, 416, 842, 850
LweI GCATC 1 cut(s) 256
MaeI CTAG 2 cut(s) 221, 578
MaeII ACGT 3 cut(s) 121, 744, 971
MaeIII GTNAC 1 cut(s) 286
MalI GATC 6 cut(s) 6, 129, 392, 837, 948, 960
MboI GATC 6 cut(s) 4, 127, 390, 835, 946, 958
MboII GAAGA 6 cut(s) 259, 355, 565, 913, 926, 956
MflI RGATCY 2 cut(s) 4, 946
MhlI GDGCHC 1 cut(s) 73
MluCI AATT 6 cut(s) 51, 431, 483, 558, 593, 869
MmeI TCCRAC 1 cut(s) 283
MnlI CCTC 6 cut(s) 121, 507, 749, 820, 928, 1017
MseI TTAA 2 cut(s) 444, 489
MslI CAYNNNNRTG 2 cut(s) 386, 641
MspA1I CMGCKG 2 cut(s) 407, 833
MspR9I CCNGG 1 cut(s) 911
Mva1269I GAATGC 1 cut(s) 176
MvaI CCWGG 1 cut(s) 911
MvnI CGCG 1 cut(s) 998
MwoI GCNNNNNNNGC 1 cut(s) 683
NcoI CCATGG 1 cut(s) 642
NdeII GATC 6 cut(s) 4, 127, 390, 835, 946, 958
NlaIII CATG 1 cut(s) 646
NlaIV GGNNCC 3 cut(s) 6, 230, 715
NmeAIII GCCGAG 1 cut(s) 775
NmuCI GTSAC 1 cut(s) 286
PctI GAATGC 1 cut(s) 176
PfeI GAWTC 2 cut(s) 608, 793
PkrI GCNGC 4 cut(s) 175, 406, 832, 865
Ppu21I YACGTR 1 cut(s) 122
PshAI GACNNNNGTC 1 cut(s) 299
Psp1406I AACGTT 1 cut(s) 971
Psp6I CCWGG 1 cut(s) 909
PspGI CCWGG 1 cut(s) 909
PspN4I GGNNCC 3 cut(s) 6, 230, 715
PspPI GGNCC 1 cut(s) 116
PsuI RGATCY 2 cut(s) 4, 946
PvuII CAGCTG 2 cut(s) 407, 833
RsaI GTAC 4 cut(s) 124, 518, 631, 715
RsaNI GTAC 4 cut(s) 123, 517, 630, 714
RseI CAYNNNNRTG 2 cut(s) 386, 641
SaqAI TTAA 2 cut(s) 444, 489
SatI GCNGC 4 cut(s) 174, 405, 831, 864
Sau3AI GATC 6 cut(s) 4, 127, 390, 835, 946, 958
Sau96I GGNCC 1 cut(s) 116
ScrFI CCNGG 1 cut(s) 911
SduI GDGCHC 1 cut(s) 73
SfaNI GCATC 1 cut(s) 256
SinI GGWCC 1 cut(s) 116
SmiMI CAYNNNNRTG 2 cut(s) 386, 641
SmlI CTYRAG 4 cut(s) 236, 394, 672, 887
SmoI CTYRAG 4 cut(s) 236, 394, 672, 887
Sse9I AATT 6 cut(s) 51, 431, 483, 558, 593, 869
SspMI CTAG 2 cut(s) 221, 578
StyD4I CCNGG 1 cut(s) 909
StyI CCWWGG 1 cut(s) 642
TaaI ACNGT 5 cut(s) 187, 208, 301, 509, 586
TaiI ACGT 3 cut(s) 124, 747, 974
TaqI TCGA 3 cut(s) 16, 498, 1014
TasI AATT 6 cut(s) 51, 431, 483, 558, 593, 869
TfiI GAWTC 2 cut(s) 608, 793
Tru1I TTAA 2 cut(s) 444, 489
Tru9I TTAA 2 cut(s) 444, 489
TscAI CASTG 1 cut(s) 304
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 4 cut(s) 173, 404, 830, 863
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 1 cut(s) 600
TspRI CASTG 1 cut(s) 304
VpaK11BI GGWCC 1 cut(s) 116
XapI RAATTY 3 cut(s) 51, 483, 869
XcmI CCANNNNNNNNNTGG 1 cut(s) 640
XmiI GTMKAC 1 cut(s) 189
XspI CTAG 2 cut(s) 221, 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.