Rorug05G0283100

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002844
Physical Location & Seq
Reverse (-)
32418717 .. 32421554
2838 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0283100.1

Sequence Viewer

Length: 954 bp
ATGAAGATGGTGAAGAACCAGCAGTTGGTCTACAGCTGTATTCTTTTGTTCATCTGTTTGATTGATTTAAGCAATGCAGCTCGCAGTAATCCCACTGACGATGTTTTGGAATGGGTTCAAAAAGATGATCGCCGTTTCCTGCGTGCCAATATCAGAGTTGGTGATCTTAATCGAACCATTAAGTTTTACACCGAGTTTTTTGGGATGGAAGTGTTGAGCAGAAAAGATTTTCCAAAAGTAAATATCTCAAATGCAGTCATTGGGTTTGGATCTGAAGAGACTCAATTTGTTCTGGGACTCACCCAACACCACAGTGCAGTTGACAAATTGGAGCTCGGAACAGCCTTCCATCATTTCGGAATTACAACTCAAGATATTTATACAATGGTTGAAAAAATTCGAGCGGCTGGTGGGGTGATCACTCGTGAACCAGGGCCAGTTGCACCCGGCGGCACTACCATCTACGCCTTTGTGAACGATCCCGATGGCTACAGTTTCGAGCTCTTCCTCAGGCCGCCAACTCCAGAACCAATTAATCATATATGTCTTAATGTGGTTGACATTGATCGTTCTATTGACTTCTACCAAAAGGCTTTGGGAATGAACTTACTAGTGCCAAAGTACAACAATACTCAAGAGCAGTATACTGTAGCTATGGTGGGCTATGGATCCAACTATACTCAGACGACCGTCATCGAGCTGCAATATAACTATAATGTGACAGATTATACCAAAGGAAATGGATATGCACAGGTTGCTATTGGCACCGATGATGTATACAAGAGTGCAGCAGCTGTTGAACAGGTTATCCAGGAACTTGGTGGGAAAATAATTCAACCACCAGGTCCAATTCCATTTATCAAGACCAAAATCACTGCATTCCTTGATTCAGATGGCTTTGAAACGGTGTTGGTGGACAATGAAGATTATCTTAAATATCTGAAGGAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0003824 GO:0004788 GO:0005215 GO:0005216 GO:0005242 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005887 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006873 GO:0006875 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009108 GO:0009229 GO:0009705 GO:0009987 GO:0010029 GO:0010119 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015276 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016043 GO:0016740 GO:0016772 GO:0016778 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019725 GO:0022607 GO:0022803 GO:0022832 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022840 GO:0022841 GO:0022842 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0030004 GO:0030007 GO:0030322 GO:0031004 GO:0031090 GO:0031224 GO:0031226 GO:0032991 GO:0034220 GO:0034641 GO:0042357 GO:0042391 GO:0042592 GO:0042723 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046483 GO:0046873 GO:0048580 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050801 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051239 GO:0051259 GO:0051260 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0055085 GO:0065003 GO:0065007 GO:0065008 GO:0071704 GO:0071804 GO:0071805 GO:0071840 GO:0071944 GO:0072527 GO:0072528 GO:0090407 GO:0090533 GO:0098533 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0099094 GO:1900140 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902494 GO:1902495 GO:1904949 GO:1990351 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

317

Amino Acids

35.62

Weight (kDa)

5.0

Isoelectric Point (pI)

31.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 47 - 168 5.8e-18 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 51 - 149 3.8e-06 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase PF00903 178 - 301 8.8e-13 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 764
AccB7I CCANNNNNTGG 1 cut(s) 25
AccBSI CCGCTC 1 cut(s) 404
AccI GTMKAC 3 cut(s) 30, 644, 777
AciI CCGC 3 cut(s) 404, 450, 515
AclWI GGATC 4 cut(s) 277, 473, 663, 676
AcsI RAATTY 1 cut(s) 396
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 1 cut(s) 623
AfiI CCNNNNNNNGG 1 cut(s) 25
AgsI TTSAA 5 cut(s) 119, 392, 800, 836, 902
AhlI ACTAGT 1 cut(s) 610
AjnI CCWGG 3 cut(s) 430, 810, 841
AjuI GAANNNNNNNTTGG 2 cut(s) 8, 40
AleI CACNNNNGTG 1 cut(s) 312
AloI GAACNNNNNNTCC 2 cut(s) 792, 824
AluBI AGCT 7 cut(s) 36, 80, 334, 502, 653, 700, 794
AluI AGCT 7 cut(s) 36, 80, 334, 502, 653, 700, 794
Alw21I GWGCWC 2 cut(s) 336, 504
Alw26I GTCTC 1 cut(s) 272
AlwI GGATC 4 cut(s) 277, 473, 663, 676
AlwNI CAGNNNCTG 1 cut(s) 794
AoxI GGCC 2 cut(s) 434, 512
ApeKI GCWGC 4 cut(s) 77, 700, 788, 791
ApoI RAATTY 1 cut(s) 396
AseI ATTAAT 1 cut(s) 534
Asp700I GAANNNNTTC 2 cut(s) 114, 396
AspS9I GGNCC 2 cut(s) 434, 845
AsuC2I CCSGG 1 cut(s) 447
AsuHPI GGTGA 4 cut(s) 22, 173, 292, 427
AvaII GGWCC 1 cut(s) 845
AxyI CCTNAGG 1 cut(s) 509
BamHI GGATCC 1 cut(s) 668
BanI GGYRCC 1 cut(s) 764
BanII GRGCYC 2 cut(s) 336, 504
BauI CACGAG 1 cut(s) 423
Bbv12I GWGCWC 2 cut(s) 336, 504
BbvI GCAGC 4 cut(s) 89, 687, 800, 803
BccI CCATC 5 cut(s) 199, 357, 467, 479, 887
BceAI ACGGC 1 cut(s) 117
BciT130I CCWGG 3 cut(s) 432, 812, 843
BclI TGATCA 1 cut(s) 417
BcnI CCSGG 1 cut(s) 447
BcoDI GTCTC 1 cut(s) 272
BcuI ACTAGT 1 cut(s) 610
BfaI CTAG 1 cut(s) 611
BfmI CTRYAG 3 cut(s) 31, 490, 648
BisI GCNGC 7 cut(s) 78, 405, 451, 515, 701, 789, 792
BlsI GCNGC 7 cut(s) 79, 406, 452, 516, 702, 790, 793
Bme1390I CCNGG 4 cut(s) 432, 447, 812, 843
Bme18I GGWCC 1 cut(s) 845
BmgT120I GGNCC 2 cut(s) 434, 845
BmiI GGNNCC 2 cut(s) 670, 766
BmrFI CCNGG 4 cut(s) 432, 447, 812, 843
BoxI GACNNNNGTC 1 cut(s) 689
BpmI CTGGAG 1 cut(s) 507
BpuEI CTTGAG 2 cut(s) 354, 618
BpuMI CCSGG 1 cut(s) 447
BsaBI GATNNNNATC 1 cut(s) 168
BsaJI CCNNGG 1 cut(s) 431
Bsc4I CCNNNNNNNGG 1 cut(s) 25
Bse1I ACTGG 1 cut(s) 437
Bse21I CCTNAGG 1 cut(s) 509
Bse3DI GCAATG 1 cut(s) 79
Bse8I GATNNNNATC 1 cut(s) 168
BseBI CCWGG 3 cut(s) 432, 812, 843
BseDI CCNNGG 1 cut(s) 431
BseGI GGATG 1 cut(s) 210
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 1 cut(s) 25
BseMI GCAATG 1 cut(s) 79
BseMII CTCAG 2 cut(s) 523, 695
BseNI ACTGG 1 cut(s) 437
BseXI GCAGC 4 cut(s) 89, 687, 800, 803
BsgI GTGCAG 2 cut(s) 336, 807
Bsh1285I CGRYCG 1 cut(s) 690
BshFI GGCC 2 cut(s) 436, 514
BshNI GGYRCC 1 cut(s) 764
BsiEI CGRYCG 1 cut(s) 690
BsiHKAI GWGCWC 2 cut(s) 336, 504
BsiSI CCGG 1 cut(s) 447
BslFI GGGAC 1 cut(s) 309
BslI CCNNNNNNNGG 1 cut(s) 25
BsmAI GTCTC 1 cut(s) 272
BsmFI GGGAC 1 cut(s) 309
BsmI GAATGC 1 cut(s) 878
BsnI GGCC 2 cut(s) 436, 514
Bsp1286I GDGCHC 2 cut(s) 336, 504
Bsp143I GATC 7 cut(s) 127, 163, 269, 417, 478, 565, 668
BspACI CCGC 3 cut(s) 404, 450, 515
BspANI GGCC 2 cut(s) 436, 514
BspCNI CTCAG 2 cut(s) 522, 694
BspLI GGNNCC 2 cut(s) 670, 766
BspPI GGATC 4 cut(s) 277, 473, 663, 676
BspQI GCTCTTC 1 cut(s) 509
BspT107I GGYRCC 1 cut(s) 764
BsrBI CCGCTC 1 cut(s) 404
BsrDI GCAATG 1 cut(s) 79
BsrI ACTGG 1 cut(s) 437
BssECI CCNNGG 1 cut(s) 431
BssMI GATC 7 cut(s) 127, 163, 269, 417, 478, 565, 668
BssNAI GTATAC 2 cut(s) 645, 778
BssSI CACGAG 1 cut(s) 423
Bst1107I GTATAC 2 cut(s) 645, 778
Bst2BI CACGAG 1 cut(s) 423
Bst2UI CCWGG 3 cut(s) 432, 812, 843
Bst4CI ACNGT 5 cut(s) 314, 494, 649, 691, 907
Bst6I CTCTTC 3 cut(s) 270, 509, 942
BstAPI GCANNNNNTGC 1 cut(s) 755
BstC8I GCNNGC 2 cut(s) 82, 144
BstDEI CTNAG 2 cut(s) 509, 681
BstF5I GGATG 1 cut(s) 210
BstKTI GATC 7 cut(s) 130, 166, 272, 420, 481, 568, 671
BstMAI GTCTC 1 cut(s) 272
BstMBI GATC 7 cut(s) 127, 163, 269, 417, 478, 565, 668
BstMCI CGRYCG 1 cut(s) 690
BstMWI GCNNNNNNNGC 1 cut(s) 755
BstNI CCWGG 3 cut(s) 432, 812, 843
BstPAI GACNNNNGTC 1 cut(s) 689
BstSCI CCNGG 4 cut(s) 430, 445, 810, 841
BstSFI CTRYAG 3 cut(s) 31, 490, 648
BstV1I GCAGC 4 cut(s) 89, 687, 800, 803
BstX2I RGATCY 2 cut(s) 269, 668
BstXI CCANNNNNNTGG 1 cut(s) 818
BstYI RGATCY 2 cut(s) 269, 668
BstZ17I GTATAC 2 cut(s) 645, 778
Bsu36I CCTNAGG 1 cut(s) 509
BsuRI GGCC 2 cut(s) 436, 514
BtsCI GGATG 1 cut(s) 210
BtsI GCAGTG 1 cut(s) 873
BtsIMutI CAGTG 3 cut(s) 93, 319, 873
Cac8I GCNNGC 2 cut(s) 82, 144
CaiI CAGNNNCTG 1 cut(s) 794
Cfr13I GGNCC 2 cut(s) 434, 845
CsiI ACCWGGT 1 cut(s) 841
Csp6I GTAC 1 cut(s) 622
CviQI GTAC 1 cut(s) 622
DdeI CTNAG 2 cut(s) 509, 681
DpnI GATC 7 cut(s) 129, 165, 271, 419, 480, 567, 670
DpnII GATC 7 cut(s) 127, 163, 269, 417, 478, 565, 668
Eam1104I CTCTTC 3 cut(s) 270, 509, 942
EarI CTCTTC 3 cut(s) 270, 509, 942
Ecl136II GAGCTC 2 cut(s) 334, 502
Eco24I GRGCYC 2 cut(s) 336, 504
Eco47I GGWCC 1 cut(s) 845
Eco53kI GAGCTC 2 cut(s) 334, 502
Eco57I CTGAAG 1 cut(s) 294
Eco81I CCTNAGG 1 cut(s) 509
EcoICRI GAGCTC 2 cut(s) 334, 502
EcoRII CCWGG 3 cut(s) 430, 810, 841
EcoT38I GRGCYC 2 cut(s) 336, 504
FalI AAGNNNNNCTT 2 cut(s) 915, 947
FaqI GGGAC 1 cut(s) 309
FbaI TGATCA 1 cut(s) 417
FblI GTMKAC 3 cut(s) 30, 644, 777
Fnu4HI GCNGC 7 cut(s) 78, 405, 451, 515, 701, 789, 792
FokI GGATG 1 cut(s) 217
FriOI GRGCYC 2 cut(s) 336, 504
Fsp4HI GCNGC 7 cut(s) 78, 405, 451, 515, 701, 789, 792
FspBI CTAG 1 cut(s) 611
GluI GCNGC 7 cut(s) 78, 405, 451, 515, 701, 789, 792
GsuI CTGGAG 1 cut(s) 507
HaeIII GGCC 2 cut(s) 436, 514
HapII CCGG 1 cut(s) 447
HincII GTYRAC 2 cut(s) 322, 559
HindII GTYRAC 2 cut(s) 322, 559
HinfI GANTC 3 cut(s) 280, 297, 887
HpaII CCGG 1 cut(s) 447
HphI GGTGA 4 cut(s) 22, 173, 292, 427
Hpy166II GTNNAC 8 cut(s) 31, 322, 428, 475, 559, 645, 778, 916
Hpy188I TCNGA 7 cut(s) 155, 274, 338, 359, 684, 892, 942
Hpy188III TCNNGA 6 cut(s) 371, 425, 482, 524, 635, 862
Hpy8I GTNNAC 8 cut(s) 31, 322, 428, 475, 559, 645, 778, 916
HpyAV CCTTC 2 cut(s) 355, 937
HpyCH4III ACNGT 5 cut(s) 314, 494, 649, 691, 907
HpyCH4V TGCA 8 cut(s) 77, 254, 317, 443, 703, 749, 788, 878
HpyF10VI GCNNNNNNNGC 1 cut(s) 755
HpyF3I CTNAG 2 cut(s) 509, 681
Ksp22I TGATCA 1 cut(s) 417
Kzo9I GATC 7 cut(s) 127, 163, 269, 417, 478, 565, 668
LguI GCTCTTC 1 cut(s) 509
LmnI GCTCC 1 cut(s) 331
Lsp1109I GCAGC 4 cut(s) 89, 687, 800, 803
MabI ACCWGGT 1 cut(s) 841
MaeI CTAG 1 cut(s) 611
MaeIII GTNAC 1 cut(s) 718
MalI GATC 7 cut(s) 129, 165, 271, 419, 480, 567, 670
MbiI CCGCTC 1 cut(s) 404
MboI GATC 7 cut(s) 127, 163, 269, 417, 478, 565, 668
MboII GAAGA 5 cut(s) 16, 25, 287, 496, 935
MflI RGATCY 2 cut(s) 269, 668
MhlI GDGCHC 2 cut(s) 336, 504
MluCI AATT 7 cut(s) 284, 326, 360, 396, 531, 831, 849
MlyI GAGTC 2 cut(s) 274, 291
MmeI TCCRAC 1 cut(s) 696
MnlI CCTC 1 cut(s) 518
MroXI GAANNNNTTC 2 cut(s) 114, 396
MseI TTAA 6 cut(s) 68, 168, 180, 534, 549, 933
MslI CAYNNNNRTG 1 cut(s) 312
MspA1I CMGCKG 2 cut(s) 36, 794
MspI CCGG 1 cut(s) 447
MspR9I CCNGG 4 cut(s) 432, 447, 812, 843
Mva1269I GAATGC 1 cut(s) 878
MvaI CCWGG 3 cut(s) 432, 812, 843
MwoI GCNNNNNNNGC 1 cut(s) 755
NciI CCSGG 1 cut(s) 447
NdeII GATC 7 cut(s) 127, 163, 269, 417, 478, 565, 668
NlaIV GGNNCC 2 cut(s) 670, 766
NmuCI GTSAC 1 cut(s) 718
OliI CACNNNNGTG 1 cut(s) 312
PciSI GCTCTTC 1 cut(s) 509
PctI GAATGC 1 cut(s) 878
PdmI GAANNNNTTC 2 cut(s) 114, 396
PfeI GAWTC 1 cut(s) 887
PflMI CCANNNNNTGG 1 cut(s) 25
PfoI TCCNGGA 1 cut(s) 810
PkrI GCNGC 7 cut(s) 79, 406, 452, 516, 702, 790, 793
PleI GAGTC 2 cut(s) 274, 291
PpsI GAGTC 2 cut(s) 274, 291
PshAI GACNNNNGTC 1 cut(s) 689
PshBI ATTAAT 1 cut(s) 534
Psp124BI GAGCTC 2 cut(s) 336, 504
Psp6I CCWGG 3 cut(s) 430, 810, 841
PspGI CCWGG 3 cut(s) 430, 810, 841
PspN4I GGNNCC 2 cut(s) 670, 766
PspPI GGNCC 2 cut(s) 434, 845
PstNI CAGNNNCTG 1 cut(s) 794
PsuI RGATCY 2 cut(s) 269, 668
PvuII CAGCTG 2 cut(s) 36, 794
RsaI GTAC 1 cut(s) 623
RsaNI GTAC 1 cut(s) 622
RseI CAYNNNNRTG 1 cut(s) 312
SacI GAGCTC 2 cut(s) 336, 504
SapI GCTCTTC 1 cut(s) 509
SaqAI TTAA 6 cut(s) 68, 168, 180, 534, 549, 933
SatI GCNGC 7 cut(s) 78, 405, 451, 515, 701, 789, 792
Sau3AI GATC 7 cut(s) 127, 163, 269, 417, 478, 565, 668
Sau96I GGNCC 2 cut(s) 434, 845
SchI GAGTC 2 cut(s) 274, 291
ScrFI CCNGG 4 cut(s) 432, 447, 812, 843
SduI GDGCHC 2 cut(s) 336, 504
SexAI ACCWGGT 1 cut(s) 841
SfcI CTRYAG 3 cut(s) 31, 490, 648
SinI GGWCC 1 cut(s) 845
SmiMI CAYNNNNRTG 1 cut(s) 312
SmlI CTYRAG 2 cut(s) 369, 633
SmoI CTYRAG 2 cut(s) 369, 633
SpeI ACTAGT 1 cut(s) 610
Sse9I AATT 7 cut(s) 284, 326, 360, 396, 531, 831, 849
SsiI CCGC 3 cut(s) 404, 450, 515
SspMI CTAG 1 cut(s) 611
SstI GAGCTC 2 cut(s) 336, 504
StyD4I CCNGG 4 cut(s) 430, 445, 810, 841
TaaI ACNGT 5 cut(s) 314, 494, 649, 691, 907
TaqI TCGA 4 cut(s) 172, 400, 498, 696
TasI AATT 7 cut(s) 284, 326, 360, 396, 531, 831, 849
TatI WGTACW 1 cut(s) 621
TauI GCSGC 3 cut(s) 407, 453, 517
TfiI GAWTC 1 cut(s) 887
Tru1I TTAA 6 cut(s) 68, 168, 180, 534, 549, 933
Tru9I TTAA 6 cut(s) 68, 168, 180, 534, 549, 933
TscAI CASTG 3 cut(s) 100, 319, 880
TseFI GTSAC 1 cut(s) 718
TseI GCWGC 4 cut(s) 77, 700, 788, 791
Tsp45I GTSAC 1 cut(s) 718
TspDTI ATGAA 4 cut(s) 17, 40, 617, 936
TspRI CASTG 3 cut(s) 100, 319, 880
Van91I CCANNNNNTGG 1 cut(s) 25
VpaK11BI GGWCC 1 cut(s) 845
VspI ATTAAT 1 cut(s) 534
XapI RAATTY 1 cut(s) 396
XcmI CCANNNNNNNNNTGG 1 cut(s) 818
XmiI GTMKAC 3 cut(s) 30, 644, 777
XmnI GAANNNNTTC 2 cut(s) 114, 396
XspI CTAG 1 cut(s) 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.