Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0004405

Physical Location & Seq

Genomic Coordinates Forward (+)

29577796 .. 29582113

Gene Structure 4318 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1215400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1116 bp

ATGAAACGAGGGTACTCGGAATCGTCCTCCACCTCACTCGGGCCTCCCCAGTCGAGATTCAAACACAACCCAGAAGGTGATGCAGAATTCTTGGAGGATGAAACCACAAAAAAATTTGCACGTAAAGTGGCCGATCATTACAGTGCAAGGACGAATCAAACTCTAGAAGAACGAGAAGCTAGTCCAATCATCCATTTAAAGAAGCTTAACAATTGGATTAAGAGTGTTTTAATCCAGCTATATGCTCGCAAAGGAGATGCTGTTCTGGATCTTGCATGTGGCAAGGGGGGTGATCTCATCAAATGGGACAAGGCAAAAATTGGGTATTATGTTGGAATTGATATCGCTGAAGGCTCGATAGAAGATTGTCGTACTCGTTACAATGGTGATGCTGACCATCATCAGCATCGTAAAAAGTTTACATTTCCTGCGCATCTTATATGTGGAGATTGCTATGAGGTTCGCCTGGATAAAGTCCTGGCTGATGATGCCCCCTTTGATGTTGTCAGTTGCCAGTTTGCCTTGCATTATTCCTGGTCTACTGAGGCACGTGCACGTCGAGCCTTGGCTAATGTATCGGCTTTACTGCGCCCTGGCGGTACTTTTATTGGAACAATGCCCGATGCAAATGTGATTATCAAAAAGCTCAGAGAAGCTGAAGGATTGGTCTTTGGTAATAGCGTCTACTGGATACGTTTTGATGAAGACTTTTCTGAAAAGAAATTCAAGTCTTCGAGCCCCTTTGGTATCAAGTACAAGTTTCACCTGGAGGATGCTGTTGATTGCCCGGAATGGATTGTTCCCTTTCATGTTTTCAAATCGTTGGTTGAAGAGTACGATATGGAGCTAGTTTTGGTGAAGAACAACCATGAATTTGTGCATGAGTACATTAAAAAACCAGAATTTGTGGAGTTAATGCGGAGGCTTGGTGCATTGGGTGATGGTAACCAAGATCAAAGTACACTATCGAATGATGAATGGGAAGTAGCTCATCTCTATTTGGCATTTGTCTTGAGGAAGCGAGGGGAACCTGATCGGACACAAGTAAACGGTAGGAGAGACATAGGGAAGATGCACATATCAAAAGAGGACGTCATGTACATAAGTAATGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001510 GO:0003674 GO:0003824 GO:0004482 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005845 GO:0006139 GO:0006370 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008173 GO:0008174 GO:0008757 GO:0009451 GO:0009452 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016556 GO:0016740 GO:0016741 GO:0032259 GO:0032991 GO:0034518 GO:0034641 GO:0036260 GO:0036265 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0080009 GO:0090304 GO:0106005 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

Pox_MCEL

Protein Analysis

372

Amino Acids

42.5

Weight (kDa)

6.29

Isoelectric Point (pI)

39.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
mRNA_G-N7_MeTrfase	PF03291	32 - 341	9.3e-129	mRNA (guanine-N(7))-methyltransferase domain
Methyltransf_23	PF13489	81 - 218	6e-06	Methyltransferase domain
Methyltransf_31	PF13847	84 - 210	1e-09	Methyltransferase domain
Methyltransf_25	PF13649	88 - 200	2.5e-11	Methyltransferase domain
Methyltransf_12	PF08242	89 - 202	1.9e-11	Methyltransferase domain
Methyltransf_11	PF08241	89 - 203	6.9e-10	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004405)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20650 AT3G20650 AT3G20650
fragaria_vesca	FvH4_3g21511 FvH4_3g21511 FvH4_3g21511 FvH4_6g24821
malus_domestica	MD03G1215400.v1.1 MD11G1013800.v1.1 MD11G1230000.v1.1
prunus_persica	Prupe.4G197400_v2.0.a1
pyrus_communis	pycom03g16380 pycom11g20380
rosa_chinensis	RchiOBHm_Chr1g0332461 RchiOBHm_Chr5g0036961
rosa_laevigata	RLG00000019767 RLG00000029593 RLG00000029668 RLG00000033739
rosa_multiflora	Rmu_sc0000574.1_g000061 Rmu_sc0006147.1_g000007 Rmu_sc0007663.1_g000020 Rmu_sc0024051.1_g000002
rosa_roxburghii	Rroxscaffold_1G00043990 Rroxscaffold_2G00105880
rosa_rugosa	Rorug01G0101300 Rorug05G0159400
rosa_samantha	Rh1DG129900 Rh5DG261500
rosa_wichuraiana	Rw1G010160 Rw1G015570 Rw5G023160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1095
Acc16I	TGCGCA	1 cut(s)	432
AccI	GTMKAC	2 cut(s)	539, 684
AciI	CCGC	2 cut(s)	597, 919
AclWI	GGATC	1 cut(s)	276
AcoI	YGGCCR	1 cut(s)	129
AcsI	RAATTY	5 cut(s)	86, 113, 722, 872, 902
AcuI	CTGAAG	2 cut(s)	369, 678
AcvI	CACGTG	1 cut(s)	551
AcyI	GRCGYC	1 cut(s)	1092
AfaI	GTAC	8 cut(s)	14, 373, 601, 755, 836, 887, 961, 1100
AfiI	CCNNNNNNNGG	1 cut(s)	39
AgsI	TTSAA	4 cut(s)	61, 727, 817, 830
AjiI	CACGTC	1 cut(s)	557
AjnI	CCWGG	5 cut(s)	465, 477, 533, 592, 765
AloI	GAACNNNNNNTCC	2 cut(s)	246, 278
AluBI	AGCT	7 cut(s)	179, 205, 238, 646, 656, 847, 989
AluI	AGCT	7 cut(s)	179, 205, 238, 646, 656, 847, 989
Alw21I	GWGCWC	1 cut(s)	556
Alw26I	GTCTC	1 cut(s)	1053
Alw44I	GTGCAC	1 cut(s)	552
AlwI	GGATC	1 cut(s)	276
Ama87I	CYCGRG	1 cut(s)	38
AoxI	GGCC	2 cut(s)	41, 129
ApaLI	GTGCAC	1 cut(s)	552
ApoI	RAATTY	5 cut(s)	86, 113, 722, 872, 902
AspLEI	GCGC	2 cut(s)	433, 591
AspS9I	GGNCC	1 cut(s)	41
AsuC2I	CCSGG	1 cut(s)	788
AsuHPI	GGTGA	6 cut(s)	89, 302, 398, 755, 868, 950
AvaI	CYCGRG	1 cut(s)	38
BaeGI	GKGCMC	1 cut(s)	556
BanII	GRGCYC	1 cut(s)	740
BbrPI	CACGTG	1 cut(s)	551
BbsI	GAAGAC	2 cut(s)	711, 723
Bbv12I	GWGCWC	1 cut(s)	556
BccI	CCATC	2 cut(s)	405, 935
BciT130I	CCWGG	5 cut(s)	467, 479, 535, 594, 767
BciVI	GTATCC	1 cut(s)	684
BcnI	CCSGG	1 cut(s)	788
BcoDI	GTCTC	1 cut(s)	1053
BfaI	CTAG	3 cut(s)	164, 180, 848
BfuI	GTATCC	1 cut(s)	684
Bme1390I	CCNGG	6 cut(s)	467, 479, 535, 594, 767, 788
BmeT110I	CYCGRG	1 cut(s)	38
BmgBI	CACGTC	1 cut(s)	557
BmgT120I	GGNCC	1 cut(s)	41
BmiI	GGNNCC	1 cut(s)	1029
BmrFI	CCNGG	6 cut(s)	467, 479, 535, 594, 767, 788
BmrI	ACTGGG	1 cut(s)	43
BmsI	GCATC	9 cut(s)	70, 247, 379, 415, 442, 478, 613, 763, 1062
BmuI	ACTGGG	1 cut(s)	43
BpiI	GAAGAC	2 cut(s)	711, 723
BpmI	CTGGAG	1 cut(s)	788
BpuEI	CTTGAG	1 cut(s)	1033
BpuMI	CCSGG	1 cut(s)	788
BsaAI	YACGTR	2 cut(s)	122, 551
BsaHI	GRCGYC	1 cut(s)	1092
BsaJI	CCNNGG	2 cut(s)	564, 592
BsaXI	ACNNNNNCTCC	4 cut(s)	246, 276, 913, 943
Bsc4I	CCNNNNNNNGG	1 cut(s)	39
Bse1I	ACTGG	3 cut(s)	49, 514, 692
BseBI	CCWGG	5 cut(s)	467, 479, 535, 594, 767
BseDI	CCNNGG	2 cut(s)	564, 592
BseGI	GGATG	3 cut(s)	103, 189, 778
BseLI	CCNNNNNNNGG	1 cut(s)	39
BseMII	CTCAG	2 cut(s)	534, 661
BseNI	ACTGG	3 cut(s)	49, 514, 692
BseSI	GKGCMC	1 cut(s)	556
BshFI	GGCC	2 cut(s)	43, 131
BsiHKAI	GWGCWC	1 cut(s)	556
BsiHKCI	CYCGRG	1 cut(s)	38
BsiSI	CCGG	1 cut(s)	788
BslFI	GGGAC	1 cut(s)	320
BslI	CCNNNNNNNGG	1 cut(s)	39
BsmAI	GTCTC	1 cut(s)	1053
BsmFI	GGGAC	1 cut(s)	320
BsnI	GGCC	2 cut(s)	43, 131
BsoBI	CYCGRG	1 cut(s)	38
Bsp1286I	GDGCHC	2 cut(s)	556, 740
Bsp1407I	TGTACA	1 cut(s)	1098
Bsp143I	GATC	5 cut(s)	133, 268, 292, 952, 1033
BspACI	CCGC	2 cut(s)	597, 919
BspANI	GGCC	2 cut(s)	43, 131
BspCNI	CTCAG	2 cut(s)	535, 660
BspLI	GGNNCC	1 cut(s)	1029
BspPI	GGATC	1 cut(s)	276
BsrGI	TGTACA	1 cut(s)	1098
BsrI	ACTGG	3 cut(s)	49, 514, 692
BssECI	CCNNGG	2 cut(s)	564, 592
BssMI	GATC	5 cut(s)	133, 268, 292, 952, 1033
BssNI	GRCGYC	1 cut(s)	1092
BssT1I	CCWWGG	1 cut(s)	564
Bst2UI	CCWGG	5 cut(s)	467, 479, 535, 594, 767
Bst4CI	ACNGT	2 cut(s)	143, 1052
Bst6I	CTCTTC	1 cut(s)	825
BstACI	GRCGYC	1 cut(s)	1092
BstAUI	TGTACA	1 cut(s)	1098
BstBAI	YACGTR	2 cut(s)	122, 551
BstC8I	GCNNGC	1 cut(s)	247
BstDEI	CTNAG	2 cut(s)	543, 647
BstEII	GGTNACC	1 cut(s)	944
BstF5I	GGATG	3 cut(s)	103, 189, 778
BstHHI	GCGC	2 cut(s)	433, 591
BstKTI	GATC	5 cut(s)	136, 271, 295, 955, 1036
BstMAI	GTCTC	1 cut(s)	1053
BstMBI	GATC	5 cut(s)	133, 268, 292, 952, 1033
BstMWI	GCNNNNNNNGC	2 cut(s)	488, 560
BstNI	CCWGG	5 cut(s)	467, 479, 535, 594, 767
BstNSI	RCATGY	1 cut(s)	279
BstPI	GGTNACC	1 cut(s)	944
BstSCI	CCNGG	6 cut(s)	465, 477, 533, 592, 765, 786
BstSLI	GKGCMC	1 cut(s)	556
BstV2I	GAAGAC	2 cut(s)	711, 723
BstX2I	RGATCY	1 cut(s)	268
BstYI	RGATCY	1 cut(s)	268
BsuI	GTATCC	1 cut(s)	684
BsuRI	GGCC	2 cut(s)	43, 131
BtrI	CACGTC	1 cut(s)	557
BtsCI	GGATG	3 cut(s)	103, 189, 778
BtsIMutI	CAGTG	1 cut(s)	148
Cac8I	GCNNGC	1 cut(s)	247
CfoI	GCGC	2 cut(s)	433, 591
Cfr13I	GGNCC	1 cut(s)	41
CseI	GACGC	1 cut(s)	670
Csp6I	GTAC	8 cut(s)	13, 372, 600, 754, 835, 886, 960, 1099
CviAII	CATG	5 cut(s)	276, 809, 869, 881, 1096
CviQI	GTAC	8 cut(s)	13, 372, 600, 754, 835, 886, 960, 1099
DdeI	CTNAG	2 cut(s)	543, 647
DpnI	GATC	5 cut(s)	135, 270, 294, 954, 1035
DpnII	GATC	5 cut(s)	133, 268, 292, 952, 1033
DraI	TTTAAA	1 cut(s)	198
EaeI	YGGCCR	1 cut(s)	129
Eam1104I	CTCTTC	1 cut(s)	825
EarI	CTCTTC	1 cut(s)	825
Eco130I	CCWWGG	1 cut(s)	564
Eco24I	GRGCYC	1 cut(s)	740
Eco32I	GATATC	1 cut(s)	343
Eco57I	CTGAAG	2 cut(s)	369, 678
Eco72I	CACGTG	1 cut(s)	551
Eco88I	CYCGRG	1 cut(s)	38
Eco91I	GGTNACC	1 cut(s)	944
EcoO65I	GGTNACC	1 cut(s)	944
EcoRI	GAATTC	1 cut(s)	86
EcoRII	CCWGG	5 cut(s)	465, 477, 533, 592, 765
EcoRV	GATATC	1 cut(s)	343
EcoT14I	CCWWGG	1 cut(s)	564
EcoT38I	GRGCYC	1 cut(s)	740
ErhI	CCWWGG	1 cut(s)	564
FaeI	CATG	5 cut(s)	279, 812, 872, 884, 1099
FaqI	GGGAC	1 cut(s)	320
FatI	CATG	5 cut(s)	275, 808, 868, 880, 1095
FblI	GTMKAC	2 cut(s)	539, 684
FokI	GGATG	3 cut(s)	110, 176, 785
FriOI	GRGCYC	1 cut(s)	740
FspBI	CTAG	3 cut(s)	164, 180, 848
FspI	TGCGCA	1 cut(s)	432
GlaI	GCGC	2 cut(s)	432, 590
GsuI	CTGGAG	1 cut(s)	788
HaeIII	GGCC	2 cut(s)	43, 131
HapII	CCGG	1 cut(s)	788
HgaI	GACGC	1 cut(s)	670
HhaI	GCGC	2 cut(s)	433, 591
Hin1I	GRCGYC	1 cut(s)	1092
Hin1II	CATG	5 cut(s)	279, 812, 872, 884, 1099
Hin6I	GCGC	2 cut(s)	431, 589
HinP1I	GCGC	2 cut(s)	431, 589
HindIII	AAGCTT	1 cut(s)	203
HinfI	GANTC	3 cut(s)	20, 57, 154
HpaII	CCGG	1 cut(s)	788
HphI	GGTGA	6 cut(s)	89, 302, 398, 755, 868, 950
Hpy166II	GTNNAC	6 cut(s)	420, 540, 554, 685, 962, 1048
Hpy188I	TCNGA	4 cut(s)	19, 650, 715, 1038
Hpy188III	TCNNGA	4 cut(s)	54, 164, 266, 1012
Hpy8I	GTNNAC	6 cut(s)	420, 540, 554, 685, 962, 1048
Hpy99I	CGWCG	1 cut(s)	561
HpyAV	CCTTC	3 cut(s)	68, 344, 653
HpyCH4III	ACNGT	2 cut(s)	143, 1052
HpyCH4IV	ACGT	5 cut(s)	121, 550, 556, 694, 1092
HpyF10VI	GCNNNNNNNGC	2 cut(s)	488, 560
HpyF3I	CTNAG	2 cut(s)	543, 647
HpySE526I	ACGT	5 cut(s)	121, 550, 556, 694, 1092
Hsp92I	GRCGYC	1 cut(s)	1092
Hsp92II	CATG	5 cut(s)	279, 812, 872, 884, 1099
HspAI	GCGC	2 cut(s)	431, 589
Kzo9I	GATC	5 cut(s)	133, 268, 292, 952, 1033
LmnI	GCTCC	1 cut(s)	844
LweI	GCATC	9 cut(s)	70, 247, 379, 415, 442, 478, 613, 763, 1062
MaeI	CTAG	3 cut(s)	164, 180, 848
MaeII	ACGT	5 cut(s)	121, 550, 556, 694, 1092
MaeIII	GTNAC	2 cut(s)	377, 944
MalI	GATC	5 cut(s)	135, 270, 294, 954, 1035
MboI	GATC	5 cut(s)	133, 268, 292, 952, 1033
MboII	GAAGA	7 cut(s)	179, 374, 716, 723, 842, 871, 1081
MfeI	CAATTG	1 cut(s)	211
MflI	RGATCY	1 cut(s)	268
MhlI	GDGCHC	2 cut(s)	556, 740
MluCI	AATT	8 cut(s)	86, 113, 211, 318, 336, 722, 872, 902
MmeI	TCCRAC	1 cut(s)	313
MseI	TTAA	6 cut(s)	197, 207, 219, 230, 891, 914
MslI	CAYNNNNRTG	1 cut(s)	141
MspI	CCGG	1 cut(s)	788
MspR9I	CCNGG	6 cut(s)	467, 479, 535, 594, 767, 788
MunI	CAATTG	1 cut(s)	211
MvaI	CCWGG	5 cut(s)	467, 479, 535, 594, 767
MwoI	GCNNNNNNNGC	2 cut(s)	488, 560
NciI	CCSGG	1 cut(s)	788
NdeII	GATC	5 cut(s)	133, 268, 292, 952, 1033
NlaIII	CATG	5 cut(s)	279, 812, 872, 884, 1099
NlaIV	GGNNCC	1 cut(s)	1029
NsbI	TGCGCA	1 cut(s)	432
NspI	RCATGY	1 cut(s)	279
PcsI	WCGNNNNNNNCGW	1 cut(s)	556
PfeI	GAWTC	3 cut(s)	20, 57, 154
PmaCI	CACGTG	1 cut(s)	551
PmlI	CACGTG	1 cut(s)	551
Ppu21I	YACGTR	2 cut(s)	122, 551
Psp6I	CCWGG	5 cut(s)	465, 477, 533, 592, 765
PspCI	CACGTG	1 cut(s)	551
PspEI	GGTNACC	1 cut(s)	944
PspGI	CCWGG	5 cut(s)	465, 477, 533, 592, 765
PspN4I	GGNNCC	1 cut(s)	1029
PspPI	GGNCC	1 cut(s)	41
PsuI	RGATCY	1 cut(s)	268
RsaI	GTAC	8 cut(s)	14, 373, 601, 755, 836, 887, 961, 1100
RsaNI	GTAC	8 cut(s)	13, 372, 600, 754, 835, 886, 960, 1099
RseI	CAYNNNNRTG	1 cut(s)	141
SaqAI	TTAA	6 cut(s)	197, 207, 219, 230, 891, 914
Sau3AI	GATC	5 cut(s)	133, 268, 292, 952, 1033
Sau96I	GGNCC	1 cut(s)	41
ScrFI	CCNGG	6 cut(s)	467, 479, 535, 594, 767, 788
SduI	GDGCHC	2 cut(s)	556, 740
SfaNI	GCATC	9 cut(s)	70, 247, 379, 415, 442, 478, 613, 763, 1062
SmiMI	CAYNNNNRTG	1 cut(s)	141
SmlI	CTYRAG	1 cut(s)	1012
SmoI	CTYRAG	1 cut(s)	1012
Sse9I	AATT	8 cut(s)	86, 113, 211, 318, 336, 722, 872, 902
SsiI	CCGC	2 cut(s)	597, 919
SspMI	CTAG	3 cut(s)	164, 180, 848
StyD4I	CCNGG	6 cut(s)	465, 477, 533, 592, 765, 786
StyI	CCWWGG	1 cut(s)	564
TaaI	ACNGT	2 cut(s)	143, 1052
TaiI	ACGT	5 cut(s)	124, 553, 559, 697, 1095
TaqI	TCGA	5 cut(s)	53, 356, 559, 734, 968
TasI	AATT	8 cut(s)	86, 113, 211, 318, 336, 722, 872, 902
TatI	WGTACW	4 cut(s)	753, 885, 959, 1098
TfiI	GAWTC	3 cut(s)	20, 57, 154
Tru1I	TTAA	6 cut(s)	197, 207, 219, 230, 891, 914
Tru9I	TTAA	6 cut(s)	197, 207, 219, 230, 891, 914
TscAI	CASTG	1 cut(s)	148
TspDTI	ATGAA	6 cut(s)	17, 114, 717, 797, 885, 990
TspRI	CASTG	1 cut(s)	148
VneI	GTGCAC	1 cut(s)	552
XapI	RAATTY	5 cut(s)	86, 113, 722, 872, 902
XbaI	TCTAGA	1 cut(s)	163
XceI	RCATGY	1 cut(s)	279
XmiI	GTMKAC	2 cut(s)	539, 684
XspI	CTAG	3 cut(s)	164, 180, 848
ZraI	GACGTC	1 cut(s)	1093

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.