MD03G1221500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003219
Physical Location & Seq
Reverse (-)
30528957 .. 30532537
3581 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1221500.v1.1.491

Sequence Viewer

Length: 477 bp
ATGGGGACTCGAGAGGTCTACGAACAGAAGCTGAGGAGTGGGAATCTGCACCACGATCCCACCATGAATCCCGGCCTCGGAACTCCTCGCTGCCCTCGCTGCCTCTCTCTCCTCGCCCACGATTCCGACAAAGGTGAATGGACCATCACTCCTGTTCTTCACGACGCCACCGCTGTTGCTGGCTCAGGTATTGGAGGAATGCTTAGTGCAATTCATAGCTTCAATACAGGGATCCCATACCTTCAGAAGCGGTTGAAGGGACCAAAGTGGTTGCCTTTCTTAGTTGGGCTTACTCCACTGCTTCTGTTTTCGGGTGCCAGTGCTACATTTGGAGGTTATTCACTTCCCAAGTTTGCTCAACTCACCGTGGCATCCTATTATGCTACCTCAAGTGCCTCACATTACGGGATTTCACTTCTTACTCGATACATCGAAGAGGCTCACACTTCCCGCTCTCGGAAAGAAGGGGTCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.13

Weight (kDa)

9.51

Isoelectric Point (pI)

32.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 314
AccBSI CCGCTC 1 cut(s) 453
AccI GTMKAC 1 cut(s) 18
AciI CCGC 3 cut(s) 171, 250, 451
AclWI GGATC 3 cut(s) 50, 226, 239
AcuI CTGAAG 1 cut(s) 227
AcyI GRCGYC 1 cut(s) 165
AfiI CCNNNNNNNGG 2 cut(s) 77, 456
AgsI TTSAA 2 cut(s) 223, 256
AluBI AGCT 2 cut(s) 31, 219
AluI AGCT 2 cut(s) 31, 219
AlwI GGATC 3 cut(s) 50, 226, 239
AlwNI CAGNNNCTG 1 cut(s) 31
Ama87I CYCGRG 1 cut(s) 9
AoxI GGCC 1 cut(s) 73
ApeKI GCWGC 2 cut(s) 90, 99
AspS9I GGNCC 2 cut(s) 141, 260
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 2 cut(s) 146, 355
AvaI CYCGRG 1 cut(s) 9
AvaII GGWCC 2 cut(s) 141, 260
BamHI GGATCC 1 cut(s) 231
BanI GGYRCC 1 cut(s) 314
BbvCI CCTCAGC 1 cut(s) 32
BbvI GCAGC 2 cut(s) 77, 86
BccI CCATC 1 cut(s) 152
BcnI CCSGG 1 cut(s) 72
BisI GCNGC 2 cut(s) 91, 100
BlsI GCNGC 2 cut(s) 92, 101
Bme1390I CCNGG 1 cut(s) 72
Bme18I GGWCC 2 cut(s) 141, 260
BmeT110I CYCGRG 1 cut(s) 9
BmgT120I GGNCC 2 cut(s) 141, 260
BmiI GGNNCC 3 cut(s) 233, 261, 316
BmrFI CCNGG 1 cut(s) 72
BmsI GCATC 1 cut(s) 380
Bpu10I CCTNAGC 2 cut(s) 32, 184
BpuEI CTTGAG 1 cut(s) 373
BpuMI CCSGG 1 cut(s) 72
BsaHI GRCGYC 1 cut(s) 165
BsaJI CCNNGG 2 cut(s) 76, 366
BsaXI ACNNNNNCTCC 2 cut(s) 133, 163
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 456
Bse1I ACTGG 1 cut(s) 318
BseDI CCNNGG 2 cut(s) 76, 366
BseGI GGATG 1 cut(s) 371
BseLI CCNNNNNNNGG 2 cut(s) 77, 456
BseMII CTCAG 2 cut(s) 23, 198
BseNI ACTGG 1 cut(s) 318
BseRI GAGGAG 3 cut(s) 49, 75, 101
BseXI GCAGC 2 cut(s) 77, 86
BsgI GTGCAG 1 cut(s) 32
BshFI GGCC 1 cut(s) 75
BshNI GGYRCC 1 cut(s) 314
BsiHKCI CYCGRG 1 cut(s) 9
BsiSI CCGG 1 cut(s) 72
BslFI GGGAC 2 cut(s) 19, 273
BslI CCNNNNNNNGG 2 cut(s) 77, 456
BsmFI GGGAC 2 cut(s) 19, 273
BsmI GAATGC 1 cut(s) 204
BsnI GGCC 1 cut(s) 75
BsoBI CYCGRG 1 cut(s) 9
Bsp143I GATC 2 cut(s) 55, 231
BspACI CCGC 3 cut(s) 171, 250, 451
BspANI GGCC 1 cut(s) 75
BspCNI CTCAG 2 cut(s) 24, 197
BspLI GGNNCC 3 cut(s) 233, 261, 316
BspPI GGATC 3 cut(s) 50, 226, 239
BspT107I GGYRCC 1 cut(s) 314
BsrBI CCGCTC 1 cut(s) 453
BsrI ACTGG 1 cut(s) 318
BssECI CCNNGG 2 cut(s) 76, 366
BssMI GATC 2 cut(s) 55, 231
BssNI GRCGYC 1 cut(s) 165
Bst4CI ACNGT 1 cut(s) 367
Bst6I CTCTTC 1 cut(s) 429
BstACI GRCGYC 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 181
BstDEI CTNAG 4 cut(s) 32, 184, 203, 280
BstDSI CCRYGG 1 cut(s) 366
BstF5I GGATG 1 cut(s) 371
BstKTI GATC 2 cut(s) 58, 234
BstMBI GATC 2 cut(s) 55, 231
BstMWI GCNNNNNNNGC 2 cut(s) 96, 99
BstSCI CCNGG 1 cut(s) 70
BstV1I GCAGC 2 cut(s) 77, 86
BstX2I RGATCY 1 cut(s) 231
BstYI RGATCY 1 cut(s) 231
BsuRI GGCC 1 cut(s) 75
BtgI CCRYGG 1 cut(s) 366
BtsCI GGATG 1 cut(s) 371
BtsI GCAGTG 1 cut(s) 296
BtsIMutI CAGTG 2 cut(s) 296, 325
Cac8I GCNNGC 1 cut(s) 181
CaiI CAGNNNCTG 1 cut(s) 31
Cfr13I GGNCC 2 cut(s) 141, 260
CseI GACGC 1 cut(s) 173
CspCI CAANNNNNGTGG 2 cut(s) 157, 192
CviAII CATG 1 cut(s) 64
CviJI RGCY 6 cut(s) 31, 75, 183, 219, 289, 440
CviKI_1 RGCY 6 cut(s) 31, 75, 183, 219, 289, 440
DdeI CTNAG 4 cut(s) 32, 184, 203, 280
DpnI GATC 2 cut(s) 57, 233
DpnII GATC 2 cut(s) 55, 231
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
Eco47I GGWCC 2 cut(s) 141, 260
Eco57I CTGAAG 1 cut(s) 227
Eco88I CYCGRG 1 cut(s) 9
FaeI CATG 1 cut(s) 67
FaiI YATR 4 cut(s) 65, 216, 238, 381
FaqI GGGAC 2 cut(s) 19, 273
FatI CATG 1 cut(s) 63
FauI CCCGC 1 cut(s) 458
FblI GTMKAC 1 cut(s) 18
Fnu4HI GCNGC 2 cut(s) 91, 100
FokI GGATG 1 cut(s) 358
Fsp4HI GCNGC 2 cut(s) 91, 100
GluI GCNGC 2 cut(s) 91, 100
HaeIII GGCC 1 cut(s) 75
HapII CCGG 1 cut(s) 72
HgaI GACGC 1 cut(s) 173
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 1 cut(s) 67
HinfI GANTC 4 cut(s) 7, 43, 67, 122
HpaII CCGG 1 cut(s) 72
HphI GGTGA 2 cut(s) 146, 355
Hpy166II GTNNAC 1 cut(s) 19
Hpy188I TCNGA 5 cut(s) 80, 127, 246, 459, 473
Hpy188III TCNNGA 2 cut(s) 11, 161
Hpy8I GTNNAC 1 cut(s) 19
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 3 cut(s) 250, 251, 458
HpyCH4III ACNGT 1 cut(s) 367
HpyCH4V TGCA 2 cut(s) 49, 209
HpyF10VI GCNNNNNNNGC 2 cut(s) 96, 99
HpyF3I CTNAG 4 cut(s) 32, 184, 203, 280
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 1 cut(s) 67
Kzo9I GATC 2 cut(s) 55, 231
LpnPI CCDG 6 cut(s) 85, 165, 165, 171, 213, 331
Lsp1109I GCAGC 2 cut(s) 77, 86
LweI GCATC 1 cut(s) 380
MalI GATC 2 cut(s) 57, 233
MbiI CCGCTC 1 cut(s) 453
MboI GATC 2 cut(s) 55, 231
MboII GAAGA 2 cut(s) 149, 446
MflI RGATCY 1 cut(s) 231
MluCI AATT 1 cut(s) 210
MmeI TCCRAC 1 cut(s) 150
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 1 cut(s) 72
MspR9I CCNGG 1 cut(s) 72
Mva1269I GAATGC 1 cut(s) 204
MwoI GCNNNNNNNGC 2 cut(s) 96, 99
NciI CCSGG 1 cut(s) 72
NdeII GATC 2 cut(s) 55, 231
NlaIII CATG 1 cut(s) 67
NlaIV GGNNCC 3 cut(s) 233, 261, 316
PaeR7I CTCGAG 1 cut(s) 9
PctI GAATGC 1 cut(s) 204
PfeI GAWTC 3 cut(s) 43, 67, 122
PkrI GCNGC 2 cut(s) 92, 101
PspN4I GGNNCC 3 cut(s) 233, 261, 316
PspPI GGNCC 2 cut(s) 141, 260
PstNI CAGNNNCTG 1 cut(s) 31
PsuI RGATCY 1 cut(s) 231
SatI GCNGC 2 cut(s) 91, 100
Sau3AI GATC 2 cut(s) 55, 231
Sau96I GGNCC 2 cut(s) 141, 260
ScrFI CCNGG 1 cut(s) 72
SetI ASST 8 cut(s) 18, 33, 136, 190, 221, 243, 337, 389
SfaNI GCATC 1 cut(s) 380
Sfr274I CTCGAG 1 cut(s) 9
SinI GGWCC 2 cut(s) 141, 260
SlaI CTCGAG 1 cut(s) 9
SmlI CTYRAG 2 cut(s) 9, 388
SmoI CTYRAG 2 cut(s) 9, 388
Sse9I AATT 1 cut(s) 210
SsiI CCGC 3 cut(s) 171, 250, 451
StyD4I CCNGG 1 cut(s) 70
TaaI ACNGT 1 cut(s) 367
TaqI TCGA 3 cut(s) 10, 424, 432
TasI AATT 1 cut(s) 210
TfiI GAWTC 3 cut(s) 43, 67, 122
TscAI CASTG 2 cut(s) 303, 325
TseI GCWGC 2 cut(s) 90, 99
TspDTI ATGAA 2 cut(s) 80, 203
TspRI CASTG 2 cut(s) 303, 325
VpaK11BI GGWCC 2 cut(s) 141, 260
XhoI CTCGAG 1 cut(s) 9
XmiI GTMKAC 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.