Rorug03G0304600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003219
Physical Location & Seq
Forward (+)
32334687 .. 32335313
627 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0304600.1

Sequence Viewer

Length: 627 bp
ATGATATCATTTACAGTGAAAGATGGAATATGGACAGTATCAGAGATCAATTCAAATCATAACCATGAGCTTGCAAAGCCTGACGAAAGACAGTTTTTGAGATCAGGGCGAAAAGTAACAGGTGCTTGTGGAAATATTTTTACTTCTATGTATGATGCGGGCATAGGACCAAAGAAATCATTTTTCTACATAGCAAATGAAATGGGTGGTCCGGAAAATGTTGGGTGCACTAAGAAGGACGTCTATAACTACTTGCAAAGGAAAAAGAATGAAATGTTGGAAGCAGGTGATGCACAAAGTTTGCTGAATCACTTTAAGCGCAAACAAGGTGAGGATCCTAACTTTTTCTACTCAATGCAATTGGACCAATATAGCCGAATGACGAATTTCTTTTGGAGGGATGGCAGAGCAAAGCTTGATTATGATTGTTTTGGGGATGTTATATGCTTTGATACAACATTTCGTACAAACAAGTATAATCTGATCTGTGCACCTATAGTTGGAGTTAATCATCATTGGAGAAATGTTTTGTTTGGATGTGCTTTTTTATTGGATGAGACTACGGAGTCATTTGTTTGGTTGTTTGAGTCATTTTTGGAGTCAATGGGAAACAAAGCACCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

208

Amino Acids

24.02

Weight (kDa)

6.95

Isoelectric Point (pI)

35.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MULE PF10551 147 - 207 4e-14 MULE transposase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 275
AasI GACNNNNNNGTC 1 cut(s) 565
AatII GACGTC 1 cut(s) 243
Acc36I ACCTGC 1 cut(s) 275
AccIII TCCGGA 1 cut(s) 211
AciI CCGC 1 cut(s) 158
AclWI GGATC 2 cut(s) 329, 342
AcsI RAATTY 1 cut(s) 385
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 1 cut(s) 466
AfiI CCNNNNNNNGG 1 cut(s) 500
AgsI TTSAA 1 cut(s) 54
AjuI GAANNNNNNNTTGG 2 cut(s) 260, 292
AluBI AGCT 2 cut(s) 70, 415
AluI AGCT 2 cut(s) 70, 415
Alw21I GWGCWC 2 cut(s) 230, 493
Alw26I GTCTC 1 cut(s) 551
Alw44I GTGCAC 2 cut(s) 226, 489
AlwI GGATC 2 cut(s) 329, 342
Aor13HI TCCGGA 1 cut(s) 211
ApaLI GTGCAC 2 cut(s) 226, 489
ApoI RAATTY 1 cut(s) 385
AspLEI GCGC 1 cut(s) 321
AspS9I GGNCC 3 cut(s) 167, 209, 364
AsuHPI GGTGA 2 cut(s) 299, 341
AvaII GGWCC 3 cut(s) 167, 209, 364
BaeGI GKGCMC 2 cut(s) 230, 493
BamHI GGATCC 1 cut(s) 334
Bbv12I GWGCWC 2 cut(s) 230, 493
BccI CCATC 2 cut(s) 17, 395
BcoDI GTCTC 1 cut(s) 551
BfmI CTRYAG 1 cut(s) 495
BfuAI ACCTGC 1 cut(s) 275
Bme18I GGWCC 3 cut(s) 167, 209, 364
BmgT120I GGNCC 3 cut(s) 167, 209, 364
BmiI GGNNCC 1 cut(s) 336
BmsI GCATC 2 cut(s) 145, 280
BsaHI GRCGYC 1 cut(s) 240
BsaWI WCCGGW 1 cut(s) 211
Bsc4I CCNNNNNNNGG 1 cut(s) 500
BseAI TCCGGA 1 cut(s) 211
BseGI GGATG 4 cut(s) 406, 442, 542, 559
BseLI CCNNNNNNNGG 1 cut(s) 500
BseSI GKGCMC 2 cut(s) 230, 493
BsiHKAI GWGCWC 2 cut(s) 230, 493
BsiSI CCGG 1 cut(s) 212
BslI CCNNNNNNNGG 1 cut(s) 500
BsmAI GTCTC 1 cut(s) 551
Bsp1286I GDGCHC 2 cut(s) 230, 493
Bsp13I TCCGGA 1 cut(s) 211
Bsp143I GATC 4 cut(s) 45, 101, 334, 483
BspACI CCGC 1 cut(s) 158
BspEI TCCGGA 1 cut(s) 211
BspLI GGNNCC 1 cut(s) 336
BspMI ACCTGC 1 cut(s) 275
BspPI GGATC 2 cut(s) 329, 342
BssMI GATC 4 cut(s) 45, 101, 334, 483
BssNI GRCGYC 1 cut(s) 240
Bst4CI ACNGT 3 cut(s) 16, 37, 93
BstACI GRCGYC 1 cut(s) 240
BstAPI GCANNNNNTGC 1 cut(s) 290
BstC8I GCNNGC 2 cut(s) 72, 160
BstDEI CTNAG 1 cut(s) 231
BstF5I GGATG 4 cut(s) 406, 442, 542, 559
BstHHI GCGC 1 cut(s) 321
BstKTI GATC 4 cut(s) 48, 104, 337, 486
BstMAI GTCTC 1 cut(s) 551
BstMBI GATC 4 cut(s) 45, 101, 334, 483
BstMWI GCNNNNNNNGC 2 cut(s) 76, 290
BstSFI CTRYAG 1 cut(s) 495
BstSLI GKGCMC 2 cut(s) 230, 493
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BtsCI GGATG 4 cut(s) 406, 442, 542, 559
BtsIMutI CAGTG 1 cut(s) 21
BveI ACCTGC 1 cut(s) 275
Cac8I GCNNGC 2 cut(s) 72, 160
CfoI GCGC 1 cut(s) 321
Cfr13I GGNCC 3 cut(s) 167, 209, 364
Csp6I GTAC 1 cut(s) 465
CviAII CATG 1 cut(s) 65
CviJI RGCY 4 cut(s) 70, 79, 375, 415
CviKI_1 RGCY 4 cut(s) 70, 79, 375, 415
CviQI GTAC 1 cut(s) 465
DdeI CTNAG 1 cut(s) 231
DpnI GATC 4 cut(s) 47, 103, 336, 485
DpnII GATC 4 cut(s) 45, 101, 334, 483
DrdI GACNNNNNNGTC 1 cut(s) 565
DseDI GACNNNNNNGTC 1 cut(s) 565
Eco32I GATATC 1 cut(s) 6
Eco47I GGWCC 3 cut(s) 167, 209, 364
EcoRV GATATC 1 cut(s) 6
FaeI CATG 1 cut(s) 68
FatI CATG 1 cut(s) 64
FauI CCCGC 1 cut(s) 151
FokI GGATG 4 cut(s) 413, 449, 549, 566
GlaI GCGC 1 cut(s) 320
HapII CCGG 1 cut(s) 212
HhaI GCGC 1 cut(s) 321
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 1 cut(s) 68
Hin6I GCGC 1 cut(s) 319
HinP1I GCGC 1 cut(s) 319
HindIII AAGCTT 1 cut(s) 413
HinfI GANTC 4 cut(s) 307, 566, 587, 599
HpaII CCGG 1 cut(s) 212
HphI GGTGA 2 cut(s) 299, 341
Hpy166II GTNNAC 2 cut(s) 228, 491
Hpy188I TCNGA 2 cut(s) 43, 483
Hpy188III TCNNGA 1 cut(s) 212
Hpy8I GTNNAC 2 cut(s) 228, 491
HpyAV CCTTC 1 cut(s) 229
HpyCH4III ACNGT 3 cut(s) 16, 37, 93
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 6 cut(s) 74, 228, 256, 293, 358, 491
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 290
HpyF3I CTNAG 1 cut(s) 231
HpySE526I ACGT 1 cut(s) 240
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 1 cut(s) 68
HspAI GCGC 1 cut(s) 319
Kpn2I TCCGGA 1 cut(s) 211
Kzo9I GATC 4 cut(s) 45, 101, 334, 483
LpnPI CCDG 5 cut(s) 90, 93, 105, 225, 270
LweI GCATC 2 cut(s) 145, 280
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 1 cut(s) 115
MalI GATC 4 cut(s) 47, 103, 336, 485
MboI GATC 4 cut(s) 45, 101, 334, 483
MfeI CAATTG 1 cut(s) 359
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 2 cut(s) 230, 493
MluCI AATT 3 cut(s) 49, 359, 385
MlyI GAGTC 3 cut(s) 575, 596, 608
MmeI TCCRAC 2 cut(s) 258, 481
MnlI CCTC 2 cut(s) 325, 390
MroI TCCGGA 1 cut(s) 211
MseI TTAA 2 cut(s) 315, 507
MslI CAYNNNNRTG 2 cut(s) 63, 622
MspI CCGG 1 cut(s) 212
MunI CAATTG 1 cut(s) 359
MwoI GCNNNNNNNGC 2 cut(s) 76, 290
NdeII GATC 4 cut(s) 45, 101, 334, 483
NlaIII CATG 1 cut(s) 68
NlaIV GGNNCC 1 cut(s) 336
PaqCI CACCTGC 1 cut(s) 275
PfeI GAWTC 1 cut(s) 307
PleI GAGTC 3 cut(s) 574, 595, 607
PpsI GAGTC 3 cut(s) 574, 595, 607
PspN4I GGNNCC 1 cut(s) 336
PspPI GGNCC 3 cut(s) 167, 209, 364
PsuI RGATCY 1 cut(s) 334
RsaI GTAC 1 cut(s) 466
RsaNI GTAC 1 cut(s) 465
RseI CAYNNNNRTG 2 cut(s) 63, 622
SaqAI TTAA 2 cut(s) 315, 507
Sau3AI GATC 4 cut(s) 45, 101, 334, 483
Sau96I GGNCC 3 cut(s) 167, 209, 364
SchI GAGTC 3 cut(s) 575, 596, 608
SduI GDGCHC 2 cut(s) 230, 493
SetI ASST 7 cut(s) 72, 124, 243, 289, 331, 417, 496
SfaNI GCATC 2 cut(s) 145, 280
SfcI CTRYAG 1 cut(s) 495
SinI GGWCC 3 cut(s) 167, 209, 364
SmiMI CAYNNNNRTG 2 cut(s) 63, 622
Sse9I AATT 3 cut(s) 49, 359, 385
SsiI CCGC 1 cut(s) 158
SspI AATATT 1 cut(s) 136
TaaI ACNGT 3 cut(s) 16, 37, 93
TaiI ACGT 1 cut(s) 243
TasI AATT 3 cut(s) 49, 359, 385
TfiI GAWTC 1 cut(s) 307
Tru1I TTAA 2 cut(s) 315, 507
Tru9I TTAA 2 cut(s) 315, 507
TscAI CASTG 1 cut(s) 21
TspDTI ATGAA 2 cut(s) 213, 285
TspGWI ACGGA 1 cut(s) 578
TspRI CASTG 1 cut(s) 21
VneI GTGCAC 2 cut(s) 226, 489
VpaK11BI GGWCC 3 cut(s) 167, 209, 364
XapI RAATTY 1 cut(s) 385
ZraI GACGTC 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.