Rorug03G0304400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003219
Physical Location & Seq
Reverse (-)
32277550 .. 32278950
1401 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0304400.1

Sequence Viewer

Length: 486 bp
ATGGTGGGCTATGTATCCTCCAGGTGGAGTTTATGCTCATCCGGGTATGGTCATGATATGATGTTCTTCCTCCGATGGAGTTTCACAAAGGCTCCCCTCATGGTTTTAGATAGCCCGATTAATAAAGCTGGGAAGTTGCGATGTGTGTATATGAAGACCGAGGATGGTAAACTTATTGAGATTAAACCATATGTTCGTATCCCAAGGTTTCATGTTGGTTTGTTTAATCCTGCCATTCACCTTGCAGTGCAGCTGCTTCGGAAACTGAGTATATCTGCCGTTGGTAAGCCTGAGAAACTTATGCGTATGATCAAGAACCCTGTGACACAATATTTACCTATGAACTCCCGGAAAGTAGGACTCTCGTACAGTTCAGAGAAATTAGTTAGCATTCAAAAATATGTTGCTGCTGCTGAAAACAATATGGACCTTGTTTTTGTGGTTGGTGCAATGGCTTATGGCAAAGTTGAGACAATGGGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

161

Amino Acids

18.12

Weight (kDa)

9.86

Isoelectric Point (pI)

32.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMG1 PF03587 33 - 157 1.9e-29 EMG1/NEP1 methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 368
AfiI CCNNNNNNNGG 1 cut(s) 24
AgsI TTSAA 1 cut(s) 395
AjnI CCWGG 1 cut(s) 20
AluBI AGCT 2 cut(s) 128, 253
AluI AGCT 2 cut(s) 128, 253
Alw26I GTCTC 1 cut(s) 464
ApeKI GCWGC 4 cut(s) 250, 253, 407, 410
ArsI GACNNNNNNTTYG 2 cut(s) 419, 451
AseI ATTAAT 1 cut(s) 120
AspS9I GGNCC 1 cut(s) 427
AsuC2I CCSGG 2 cut(s) 43, 349
AsuHPI GGTGA 1 cut(s) 230
AvaII GGWCC 1 cut(s) 427
BbsI GAAGAC 1 cut(s) 161
BbvI GCAGC 4 cut(s) 240, 262, 394, 397
BccI CCATC 2 cut(s) 69, 158
BceAI ACGGC 1 cut(s) 263
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BciT130I CCWGG 1 cut(s) 22
BciVI GTATCC 2 cut(s) 25, 209
BclI TGATCA 1 cut(s) 309
BcnI CCSGG 2 cut(s) 43, 349
BcoDI GTCTC 1 cut(s) 464
BfuI GTATCC 2 cut(s) 25, 209
BisI GCNGC 4 cut(s) 251, 254, 408, 411
BlsI GCNGC 4 cut(s) 252, 255, 409, 412
Bme1390I CCNGG 3 cut(s) 22, 43, 349
Bme18I GGWCC 1 cut(s) 427
BmgT120I GGNCC 1 cut(s) 427
BmiI GGNNCC 1 cut(s) 93
BmrFI CCNGG 3 cut(s) 22, 43, 349
BpiI GAAGAC 1 cut(s) 161
BpmI CTGGAG 1 cut(s) 4
BpuMI CCSGG 2 cut(s) 43, 349
BsaJI CCNNGG 2 cut(s) 159, 203
BsaXI ACNNNNNCTCC 2 cut(s) 76, 106
Bsc4I CCNNNNNNNGG 1 cut(s) 24
Bse3DI GCAATG 1 cut(s) 456
BseBI CCWGG 1 cut(s) 22
BseDI CCNNGG 2 cut(s) 159, 203
BseGI GGATG 2 cut(s) 38, 169
BseLI CCNNNNNNNGG 1 cut(s) 24
BseMI GCAATG 1 cut(s) 456
BseMII CTCAG 2 cut(s) 257, 282
BseXI GCAGC 4 cut(s) 240, 262, 394, 397
BseYI CCCAGC 1 cut(s) 128
BsgI GTGCAG 1 cut(s) 269
BsiSI CCGG 2 cut(s) 42, 349
BslI CCNNNNNNNGG 1 cut(s) 24
BsmAI GTCTC 1 cut(s) 464
BsmI GAATGC 1 cut(s) 390
Bsp143I GATC 1 cut(s) 309
BspCNI CTCAG 2 cut(s) 258, 283
BspHI TCATGA 1 cut(s) 52
BspLI GGNNCC 1 cut(s) 93
BsrDI GCAATG 1 cut(s) 456
BssECI CCNNGG 2 cut(s) 159, 203
BssMI GATC 1 cut(s) 309
BssT1I CCWWGG 1 cut(s) 203
Bst2UI CCWGG 1 cut(s) 22
Bst4CI ACNGT 1 cut(s) 371
BstDEI CTNAG 2 cut(s) 266, 291
BstF5I GGATG 2 cut(s) 38, 169
BstKTI GATC 1 cut(s) 312
BstMAI GTCTC 1 cut(s) 464
BstMBI GATC 1 cut(s) 309
BstNI CCWGG 1 cut(s) 22
BstSCI CCNGG 3 cut(s) 20, 41, 347
BstV1I GCAGC 4 cut(s) 240, 262, 394, 397
BstV2I GAAGAC 1 cut(s) 161
BsuI GTATCC 2 cut(s) 25, 209
BtgZI GCGATG 1 cut(s) 154
BtsCI GGATG 2 cut(s) 38, 169
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 1 cut(s) 252
CciI TCATGA 1 cut(s) 52
Cfr13I GGNCC 1 cut(s) 427
Csp6I GTAC 1 cut(s) 367
CviAII CATG 3 cut(s) 53, 100, 212
CviJI RGCY 7 cut(s) 9, 92, 114, 128, 253, 289, 455
CviKI_1 RGCY 7 cut(s) 9, 92, 114, 128, 253, 289, 455
CviQI GTAC 1 cut(s) 367
DdeI CTNAG 2 cut(s) 266, 291
DpnI GATC 1 cut(s) 311
DpnII GATC 1 cut(s) 309
Eco130I CCWWGG 1 cut(s) 203
Eco47I GGWCC 1 cut(s) 427
EcoRII CCWGG 1 cut(s) 20
EcoT14I CCWWGG 1 cut(s) 203
ErhI CCWWGG 1 cut(s) 203
FaeI CATG 3 cut(s) 56, 103, 215
FatI CATG 3 cut(s) 52, 99, 211
FauNDI CATATG 1 cut(s) 190
FbaI TGATCA 1 cut(s) 309
Fnu4HI GCNGC 4 cut(s) 251, 254, 408, 411
FokI GGATG 2 cut(s) 25, 176
Fsp4HI GCNGC 4 cut(s) 251, 254, 408, 411
GluI GCNGC 4 cut(s) 251, 254, 408, 411
GsaI CCCAGC 1 cut(s) 132
GsuI CTGGAG 1 cut(s) 4
HapII CCGG 2 cut(s) 42, 349
Hin1II CATG 3 cut(s) 56, 103, 215
HinfI GANTC 1 cut(s) 360
HpaII CCGG 2 cut(s) 42, 349
HphI GGTGA 1 cut(s) 230
Hpy166II GTNNAC 1 cut(s) 170
Hpy188I TCNGA 3 cut(s) 74, 261, 376
Hpy188III TCNNGA 2 cut(s) 53, 313
Hpy8I GTNNAC 1 cut(s) 170
HpyCH4III ACNGT 1 cut(s) 371
HpyCH4V TGCA 3 cut(s) 245, 250, 449
HpyF3I CTNAG 2 cut(s) 266, 291
Hsp92II CATG 3 cut(s) 56, 103, 215
Ksp22I TGATCA 1 cut(s) 309
Kzo9I GATC 1 cut(s) 309
LmnI GCTCC 1 cut(s) 97
LpnPI CCDG 8 cut(s) 7, 34, 55, 114, 243, 303, 333, 362
Lsp1109I GCAGC 4 cut(s) 240, 262, 394, 397
MaeIII GTNAC 1 cut(s) 322
MalI GATC 1 cut(s) 311
MboI GATC 1 cut(s) 309
MboII GAAGA 2 cut(s) 58, 166
MluCI AATT 1 cut(s) 380
MlyI GAGTC 1 cut(s) 354
MnlI CCTC 4 cut(s) 28, 80, 107, 154
MseI TTAA 4 cut(s) 120, 183, 225, 484
MspA1I CMGCKG 1 cut(s) 253
MspI CCGG 2 cut(s) 42, 349
MspR9I CCNGG 3 cut(s) 22, 43, 349
Mva1269I GAATGC 1 cut(s) 390
MvaI CCWGG 1 cut(s) 22
NciI CCSGG 2 cut(s) 43, 349
NdeI CATATG 1 cut(s) 190
NdeII GATC 1 cut(s) 309
NlaIII CATG 3 cut(s) 56, 103, 215
NlaIV GGNNCC 1 cut(s) 93
NmuCI GTSAC 1 cut(s) 322
PagI TCATGA 1 cut(s) 52
PctI GAATGC 1 cut(s) 390
PfoI TCCNGGA 1 cut(s) 347
PkrI GCNGC 4 cut(s) 252, 255, 409, 412
PleI GAGTC 1 cut(s) 354
PpsI GAGTC 1 cut(s) 354
PshBI ATTAAT 1 cut(s) 120
Psp6I CCWGG 1 cut(s) 20
PspFI CCCAGC 1 cut(s) 128
PspGI CCWGG 1 cut(s) 20
PspN4I GGNNCC 1 cut(s) 93
PspPI GGNCC 1 cut(s) 427
PvuII CAGCTG 1 cut(s) 253
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
SaqAI TTAA 4 cut(s) 120, 183, 225, 484
SatI GCNGC 4 cut(s) 251, 254, 408, 411
Sau3AI GATC 1 cut(s) 309
Sau96I GGNCC 1 cut(s) 427
SchI GAGTC 1 cut(s) 354
ScrFI CCNGG 3 cut(s) 22, 43, 349
SetI ASST 7 cut(s) 26, 130, 209, 243, 255, 340, 432
SinI GGWCC 1 cut(s) 427
Sse9I AATT 1 cut(s) 380
SspI AATATT 1 cut(s) 332
StyD4I CCNGG 3 cut(s) 20, 41, 347
StyI CCWWGG 1 cut(s) 203
TaaI ACNGT 1 cut(s) 371
TaqII GACCGA 1 cut(s) 173
TasI AATT 1 cut(s) 380
Tru1I TTAA 4 cut(s) 120, 183, 225, 484
Tru9I TTAA 4 cut(s) 120, 183, 225, 484
TscAI CASTG 1 cut(s) 252
TseFI GTSAC 1 cut(s) 322
TseI GCWGC 4 cut(s) 250, 253, 407, 410
Tsp45I GTSAC 1 cut(s) 322
TspDTI ATGAA 3 cut(s) 167, 200, 356
TspRI CASTG 1 cut(s) 252
VpaK11BI GGWCC 1 cut(s) 427
VspI ATTAAT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.