Rorug03G0170900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003219
Physical Location & Seq
Forward (+)
14434421 .. 14436748
2328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0170900.1

Sequence Viewer

Length: 396 bp
ATGTCCCGCAAAGGAGGAGGAGGAGGAGGAGGAGCTCCGGCTTCACTGCCAAAGGACGTACCTTGGAGGGCCTCAAACTCCAGACCCGTCCCCAAAATCCACCACTCCCCTATTCTTCGTGTGCCCCAAAACCCCACTACCAATTATGCAATCTCCGTCATGAAGCACCCCAATCCGGTAGGAAATGGGTTGGCCAGGGACGCCATTGTGGAAGCCGCAGGACCCGATTGCATTGTTCCGGGTCAAGTCACACCCGTCAAATTACTTGGTCTCAAGGTATGGCCTATTGAAGTTGACCTACAATTTTTGGAACCCATTGGACGTGAGCTTAAGTCTATTGGAAAGTTCATGGATGATGCTGTCACTCTCATGAATAAATCATTTATAGATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

131

Amino Acids

14.01

Weight (kDa)

9.66

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 7, 216
AcoI YGGCCR 1 cut(s) 192
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 1 cut(s) 60
AfiI CCNNNNNNNGG 1 cut(s) 175
AflII CTTAAG 1 cut(s) 329
AgsI TTSAA 1 cut(s) 290
AjiI CACGTC 1 cut(s) 323
AjnI CCWGG 1 cut(s) 194
AluBI AGCT 2 cut(s) 35, 328
AluI AGCT 2 cut(s) 35, 328
Alw21I GWGCWC 1 cut(s) 37
Alw26I GTCTC 1 cut(s) 275
AoxI GGCC 3 cut(s) 69, 192, 281
AspS9I GGNCC 2 cut(s) 69, 221
AsuC2I CCSGG 1 cut(s) 240
AvaII GGWCC 1 cut(s) 221
BaeGI GKGCMC 1 cut(s) 126
BalI TGGCCA 1 cut(s) 194
BanII GRGCYC 1 cut(s) 37
BarI GAAGNNNNNNTAC 2 cut(s) 282, 314
Bbv12I GWGCWC 1 cut(s) 37
BciT130I CCWGG 1 cut(s) 196
BcnI CCSGG 1 cut(s) 240
BcoDI GTCTC 1 cut(s) 275
BfaI CTAG 1 cut(s) 394
BfrI CTTAAG 1 cut(s) 329
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
Bme1390I CCNGG 2 cut(s) 196, 240
Bme18I GGWCC 1 cut(s) 221
BmgBI CACGTC 1 cut(s) 323
BmgT120I GGNCC 2 cut(s) 69, 221
BmiI GGNNCC 2 cut(s) 223, 312
BmrFI CCNGG 2 cut(s) 196, 240
BmsI GCATC 1 cut(s) 346
BpmI CTGGAG 1 cut(s) 64
BpuEI CTTGAG 1 cut(s) 257
BpuMI CCSGG 1 cut(s) 240
BsaHI GRCGYC 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 275
BsaJI CCNNGG 2 cut(s) 62, 195
BsaWI WCCGGW 1 cut(s) 175
Bsc4I CCNNNNNNNGG 1 cut(s) 175
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 2 cut(s) 62, 195
BseGI GGATG 1 cut(s) 358
BseLI CCNNNNNNNGG 1 cut(s) 175
BseRI GAGGAG 6 cut(s) 30, 33, 36, 39, 42, 45
BseSI GKGCMC 1 cut(s) 126
BshFI GGCC 3 cut(s) 71, 194, 283
BsiHKAI GWGCWC 1 cut(s) 37
BsiSI CCGG 3 cut(s) 38, 176, 239
BslFI GGGAC 2 cut(s) 74, 212
BslI CCNNNNNNNGG 1 cut(s) 175
BsmAI GTCTC 1 cut(s) 275
BsmFI GGGAC 2 cut(s) 74, 212
BsnI GGCC 3 cut(s) 71, 194, 283
Bso31I GGTCTC 1 cut(s) 275
Bsp1286I GDGCHC 2 cut(s) 37, 126
Bsp143I GATC 1 cut(s) 388
BspACI CCGC 2 cut(s) 7, 216
BspANI GGCC 3 cut(s) 71, 194, 283
BspHI TCATGA 2 cut(s) 159, 369
BspLI GGNNCC 2 cut(s) 223, 312
BspTI CTTAAG 1 cut(s) 329
BspTNI GGTCTC 1 cut(s) 275
BssECI CCNNGG 2 cut(s) 62, 195
BssMI GATC 1 cut(s) 388
BssNI GRCGYC 1 cut(s) 201
BssT1I CCWWGG 1 cut(s) 62
Bst2UI CCWGG 1 cut(s) 196
BstACI GRCGYC 1 cut(s) 201
BstAFI CTTAAG 1 cut(s) 329
BstF5I GGATG 1 cut(s) 358
BstKTI GATC 1 cut(s) 391
BstMAI GTCTC 1 cut(s) 275
BstMBI GATC 1 cut(s) 388
BstMWI GCNNNNNNNGC 1 cut(s) 200
BstNI CCWGG 1 cut(s) 196
BstSCI CCNGG 2 cut(s) 194, 238
BstSLI GKGCMC 1 cut(s) 126
BsuRI GGCC 3 cut(s) 71, 194, 283
BtrI CACGTC 1 cut(s) 323
BtsCI GGATG 1 cut(s) 358
BtsI GCAGTG 1 cut(s) 44
BtsIMutI CAGTG 1 cut(s) 44
CciI TCATGA 2 cut(s) 159, 369
Cfr13I GGNCC 2 cut(s) 69, 221
CseI GACGC 1 cut(s) 209
Csp6I GTAC 1 cut(s) 59
CviAII CATG 3 cut(s) 160, 349, 370
CviJI RGCY 7 cut(s) 35, 41, 71, 194, 215, 283, 328
CviKI_1 RGCY 7 cut(s) 35, 41, 71, 194, 215, 283, 328
CviQI GTAC 1 cut(s) 59
DpnI GATC 1 cut(s) 390
DpnII GATC 1 cut(s) 388
EaeI YGGCCR 1 cut(s) 192
Ecl136II GAGCTC 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 62
Eco24I GRGCYC 1 cut(s) 37
Eco31I GGTCTC 1 cut(s) 275
Eco47I GGWCC 1 cut(s) 221
Eco53kI GAGCTC 1 cut(s) 35
EcoICRI GAGCTC 1 cut(s) 35
EcoO109I RGGNCCY 2 cut(s) 69, 221
EcoRII CCWGG 1 cut(s) 194
EcoT14I CCWWGG 1 cut(s) 62
EcoT38I GRGCYC 1 cut(s) 37
ErhI CCWWGG 1 cut(s) 62
FaeI CATG 3 cut(s) 163, 352, 373
FaiI YATR 6 cut(s) 147, 161, 280, 350, 371, 386
FaqI GGGAC 2 cut(s) 74, 212
FatI CATG 3 cut(s) 159, 348, 369
FauI CCCGC 1 cut(s) 14
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 1 cut(s) 365
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 1 cut(s) 216
FspBI CTAG 1 cut(s) 394
GluI GCNGC 1 cut(s) 216
GsuI CTGGAG 1 cut(s) 64
HaeIII GGCC 3 cut(s) 71, 194, 283
HapII CCGG 3 cut(s) 38, 176, 239
HgaI GACGC 1 cut(s) 209
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 3 cut(s) 163, 352, 373
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HpaII CCGG 3 cut(s) 38, 176, 239
Hpy166II GTNNAC 1 cut(s) 295
Hpy188III TCNNGA 3 cut(s) 81, 160, 370
Hpy8I GTNNAC 1 cut(s) 295
HpyCH4IV ACGT 2 cut(s) 57, 322
HpyCH4V TGCA 2 cut(s) 149, 231
HpyF10VI GCNNNNNNNGC 1 cut(s) 200
HpySE526I ACGT 2 cut(s) 57, 322
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 3 cut(s) 163, 352, 373
Kzo9I GATC 1 cut(s) 388
LmnI GCTCC 2 cut(s) 32, 40
LpnPI CCDG 7 cut(s) 51, 94, 181, 189, 204, 208, 252
LweI GCATC 1 cut(s) 346
MaeI CTAG 1 cut(s) 394
MaeII ACGT 2 cut(s) 57, 322
MaeIII GTNAC 2 cut(s) 247, 361
MalI GATC 1 cut(s) 390
MboI GATC 1 cut(s) 388
MboII GAAGA 1 cut(s) 107
MhlI GDGCHC 2 cut(s) 37, 126
MlsI TGGCCA 1 cut(s) 194
MluCI AATT 3 cut(s) 142, 260, 302
MluNI TGGCCA 1 cut(s) 194
MnlI CCTC 8 cut(s) 8, 11, 14, 17, 20, 23, 60, 82
Mox20I TGGCCA 1 cut(s) 194
MscI TGGCCA 1 cut(s) 194
MseI TTAA 1 cut(s) 330
MslI CAYNNNNRTG 1 cut(s) 368
Msp20I TGGCCA 1 cut(s) 194
MspCI CTTAAG 1 cut(s) 329
MspI CCGG 3 cut(s) 38, 176, 239
MspR9I CCNGG 2 cut(s) 196, 240
MvaI CCWGG 1 cut(s) 196
MwoI GCNNNNNNNGC 1 cut(s) 200
NciI CCSGG 1 cut(s) 240
NdeII GATC 1 cut(s) 388
NlaIII CATG 3 cut(s) 163, 352, 373
NlaIV GGNNCC 2 cut(s) 223, 312
NmuCI GTSAC 2 cut(s) 247, 361
PagI TCATGA 2 cut(s) 159, 369
PkrI GCNGC 1 cut(s) 217
PpuMI RGGWCCY 1 cut(s) 221
Psp124BI GAGCTC 1 cut(s) 37
Psp5II RGGWCCY 1 cut(s) 221
Psp6I CCWGG 1 cut(s) 194
PspGI CCWGG 1 cut(s) 194
PspN4I GGNNCC 2 cut(s) 223, 312
PspPI GGNCC 2 cut(s) 69, 221
PspPPI RGGWCCY 1 cut(s) 221
RsaI GTAC 1 cut(s) 60
RsaNI GTAC 1 cut(s) 59
RseI CAYNNNNRTG 1 cut(s) 368
SacI GAGCTC 1 cut(s) 37
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 1 cut(s) 388
Sau96I GGNCC 2 cut(s) 69, 221
ScrFI CCNGG 2 cut(s) 196, 240
SduI GDGCHC 2 cut(s) 37, 126
SetI ASST 7 cut(s) 37, 60, 64, 279, 300, 325, 330
SfaNI GCATC 1 cut(s) 346
SinI GGWCC 1 cut(s) 221
SmiMI CAYNNNNRTG 1 cut(s) 368
SmlI CTYRAG 2 cut(s) 272, 329
SmoI CTYRAG 2 cut(s) 272, 329
Sse9I AATT 3 cut(s) 142, 260, 302
SsiI CCGC 2 cut(s) 7, 216
SspMI CTAG 1 cut(s) 394
SstI GAGCTC 1 cut(s) 37
StyD4I CCNGG 2 cut(s) 194, 238
StyI CCWWGG 1 cut(s) 62
TaiI ACGT 2 cut(s) 60, 325
TasI AATT 3 cut(s) 142, 260, 302
TauI GCSGC 1 cut(s) 218
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TscAI CASTG 1 cut(s) 51
TseFI GTSAC 2 cut(s) 247, 361
Tsp45I GTSAC 2 cut(s) 247, 361
TspDTI ATGAA 3 cut(s) 176, 337, 386
TspGWI ACGGA 1 cut(s) 145
TspRI CASTG 1 cut(s) 51
Vha464I CTTAAG 1 cut(s) 329
VpaK11BI GGWCC 1 cut(s) 221
XspI CTAG 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.