MD11G1239000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003219
Physical Location & Seq
Reverse (-)
34695725 .. 34698369
2645 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1239000.v1.1.491

Sequence Viewer

Length: 477 bp
ATGGGGACTCGAGAGGTATACGAACAGAAGCTGAGGAGCGGGAACCTGCACCACGATCCCACCATGAATCCGGGCCTCGGCACCCCTCGCTGCCCTCGTTGCCTCTCTCTCCTCGACCGCTATTCCGAAAAAGGAGAATGGACCATCACCCCTGTTCTTCACGATGCCACTGCTGTTGCTGGCTCAGGTATTGGAGGAATGCTTAGTGCAATCCATAGCTTTAATACAGGGATCCCGTACCTTCAGAGTCGCTTGAAGGGACCAAAGTGGCTTCCTTTCTTAGTTGGGCTTCCTCCACTGCTACTGTTTTCGGGTGCCAGTGCTGCATTTGGAGGTTATGCACTTCCAAAGTTTGCTCAACTCACCGTGACATCCTATTATGCTACCTCAAGTGCCTCACATTACGGGATTTCACTTCTCACACGATATATCGAAGAGGCTCACGCTTCCCGCTCTCGGAAAGAAAGGGTCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.26

Weight (kDa)

9.65

Isoelectric Point (pI)

37.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 54
AccB1I GGYRCC 2 cut(s) 80, 314
AccBSI CCGCTC 2 cut(s) 39, 453
AccI GTMKAC 1 cut(s) 18
AciI CCGC 3 cut(s) 39, 118, 451
AclWI GGATC 3 cut(s) 50, 226, 239
AcuI CTGAAG 1 cut(s) 227
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 2 cut(s) 77, 456
AgsI TTSAA 1 cut(s) 256
AluBI AGCT 2 cut(s) 31, 219
AluI AGCT 2 cut(s) 31, 219
AlwI GGATC 3 cut(s) 50, 226, 239
AlwNI CAGNNNCTG 1 cut(s) 31
Ama87I CYCGRG 1 cut(s) 9
AoxI GGCC 1 cut(s) 73
ApeKI GCWGC 2 cut(s) 90, 323
AspS9I GGNCC 3 cut(s) 73, 141, 260
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 2 cut(s) 139, 355
AvaI CYCGRG 1 cut(s) 9
AvaII GGWCC 2 cut(s) 141, 260
BamHI GGATCC 1 cut(s) 231
BanI GGYRCC 2 cut(s) 80, 314
BbvCI CCTCAGC 1 cut(s) 32
BbvI GCAGC 2 cut(s) 77, 310
BccI CCATC 1 cut(s) 152
BcgI CGANNNNNNTGC 2 cut(s) 152, 186
BcnI CCSGG 1 cut(s) 72
BfuAI ACCTGC 1 cut(s) 54
BisI GCNGC 2 cut(s) 91, 324
BlsI GCNGC 2 cut(s) 92, 325
Bme1390I CCNGG 1 cut(s) 72
Bme18I GGWCC 2 cut(s) 141, 260
BmeT110I CYCGRG 1 cut(s) 9
BmgT120I GGNCC 3 cut(s) 73, 141, 260
BmiI GGNNCC 5 cut(s) 44, 82, 233, 261, 316
BmrFI CCNGG 1 cut(s) 72
BmsI GCATC 1 cut(s) 154
Bpu10I CCTNAGC 2 cut(s) 32, 184
BpuEI CTTGAG 1 cut(s) 373
BpuMI CCSGG 1 cut(s) 72
BsaJI CCNNGG 1 cut(s) 76
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 456
Bse1I ACTGG 1 cut(s) 318
BseDI CCNNGG 1 cut(s) 76
BseGI GGATG 1 cut(s) 371
BseLI CCNNNNNNNGG 2 cut(s) 77, 456
BseMII CTCAG 2 cut(s) 23, 198
BseNI ACTGG 1 cut(s) 318
BseRI GAGGAG 2 cut(s) 49, 101
BseXI GCAGC 2 cut(s) 77, 310
BsgI GTGCAG 1 cut(s) 32
Bsh1285I CGRYCG 1 cut(s) 118
BshFI GGCC 1 cut(s) 75
BshNI GGYRCC 2 cut(s) 80, 314
BsiEI CGRYCG 1 cut(s) 118
BsiHKCI CYCGRG 1 cut(s) 9
BsiSI CCGG 1 cut(s) 71
BslFI GGGAC 2 cut(s) 19, 273
BslI CCNNNNNNNGG 2 cut(s) 77, 456
BsmFI GGGAC 2 cut(s) 19, 273
BsmI GAATGC 1 cut(s) 204
BsnI GGCC 1 cut(s) 75
BsoBI CYCGRG 1 cut(s) 9
Bsp143I GATC 2 cut(s) 55, 231
BspACI CCGC 3 cut(s) 39, 118, 451
BspANI GGCC 1 cut(s) 75
BspCNI CTCAG 2 cut(s) 24, 197
BspLI GGNNCC 5 cut(s) 44, 82, 233, 261, 316
BspMI ACCTGC 1 cut(s) 54
BspPI GGATC 3 cut(s) 50, 226, 239
BspT107I GGYRCC 2 cut(s) 80, 314
BsrBI CCGCTC 2 cut(s) 39, 453
BsrI ACTGG 1 cut(s) 318
BssECI CCNNGG 1 cut(s) 76
BssMI GATC 2 cut(s) 55, 231
BssNAI GTATAC 1 cut(s) 19
Bst1107I GTATAC 1 cut(s) 19
Bst4CI ACNGT 2 cut(s) 306, 367
Bst6I CTCTTC 1 cut(s) 429
BstC8I GCNNGC 1 cut(s) 181
BstDEI CTNAG 4 cut(s) 32, 184, 203, 280
BstF5I GGATG 1 cut(s) 371
BstKTI GATC 2 cut(s) 58, 234
BstMBI GATC 2 cut(s) 55, 231
BstMCI CGRYCG 1 cut(s) 118
BstMWI GCNNNNNNNGC 3 cut(s) 87, 99, 323
BstSCI CCNGG 1 cut(s) 70
BstV1I GCAGC 2 cut(s) 77, 310
BstX2I RGATCY 1 cut(s) 231
BstYI RGATCY 1 cut(s) 231
BstZ17I GTATAC 1 cut(s) 19
BsuRI GGCC 1 cut(s) 75
BtsCI GGATG 1 cut(s) 371
BtsI GCAGTG 2 cut(s) 168, 296
BtsIMutI CAGTG 3 cut(s) 168, 296, 325
BveI ACCTGC 1 cut(s) 54
Cac8I GCNNGC 1 cut(s) 181
CaiI CAGNNNCTG 1 cut(s) 31
Cfr13I GGNCC 3 cut(s) 73, 141, 260
Csp6I GTAC 1 cut(s) 238
CspCI CAANNNNNGTGG 2 cut(s) 157, 192
CviAII CATG 1 cut(s) 64
CviJI RGCY 7 cut(s) 31, 75, 183, 219, 271, 289, 440
CviKI_1 RGCY 7 cut(s) 31, 75, 183, 219, 271, 289, 440
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 4 cut(s) 32, 184, 203, 280
DpnI GATC 2 cut(s) 57, 233
DpnII GATC 2 cut(s) 55, 231
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
Eco47I GGWCC 2 cut(s) 141, 260
Eco57I CTGAAG 1 cut(s) 227
Eco88I CYCGRG 1 cut(s) 9
FaeI CATG 1 cut(s) 67
FaiI YATR 6 cut(s) 19, 65, 216, 339, 381, 429
FaqI GGGAC 2 cut(s) 19, 273
FatI CATG 1 cut(s) 63
FauI CCCGC 2 cut(s) 32, 458
FblI GTMKAC 1 cut(s) 18
Fnu4HI GCNGC 2 cut(s) 91, 324
FokI GGATG 1 cut(s) 358
Fsp4HI GCNGC 2 cut(s) 91, 324
GluI GCNGC 2 cut(s) 91, 324
HaeIII GGCC 1 cut(s) 75
HapII CCGG 1 cut(s) 71
Hin1II CATG 1 cut(s) 67
HinfI GANTC 3 cut(s) 7, 67, 247
HpaII CCGG 1 cut(s) 71
HphI GGTGA 2 cut(s) 139, 355
Hpy166II GTNNAC 1 cut(s) 19
Hpy188I TCNGA 4 cut(s) 127, 246, 459, 473
Hpy188III TCNNGA 2 cut(s) 11, 161
Hpy8I GTNNAC 1 cut(s) 19
HpyAV CCTTC 2 cut(s) 250, 251
HpyCH4III ACNGT 2 cut(s) 306, 367
HpyCH4V TGCA 4 cut(s) 49, 209, 326, 341
HpyF10VI GCNNNNNNNGC 3 cut(s) 87, 99, 323
HpyF3I CTNAG 4 cut(s) 32, 184, 203, 280
Hsp92II CATG 1 cut(s) 67
Kzo9I GATC 2 cut(s) 55, 231
LmnI GCTCC 1 cut(s) 36
LpnPI CCDG 7 cut(s) 59, 84, 165, 165, 171, 213, 331
Lsp1109I GCAGC 2 cut(s) 77, 310
LweI GCATC 1 cut(s) 154
MaeIII GTNAC 1 cut(s) 367
MalI GATC 2 cut(s) 57, 233
MbiI CCGCTC 2 cut(s) 39, 453
MboI GATC 2 cut(s) 55, 231
MboII GAAGA 2 cut(s) 149, 446
MflI RGATCY 1 cut(s) 231
MlyI GAGTC 1 cut(s) 256
MseI TTAA 1 cut(s) 222
MspI CCGG 1 cut(s) 71
MspR9I CCNGG 1 cut(s) 72
Mva1269I GAATGC 1 cut(s) 204
MwoI GCNNNNNNNGC 3 cut(s) 87, 99, 323
NciI CCSGG 1 cut(s) 72
NdeII GATC 2 cut(s) 55, 231
NlaIII CATG 1 cut(s) 67
NlaIV GGNNCC 5 cut(s) 44, 82, 233, 261, 316
NmeAIII GCCGAG 1 cut(s) 57
NmuCI GTSAC 1 cut(s) 367
PaeR7I CTCGAG 1 cut(s) 9
PcsI WCGNNNNNNNCGW 1 cut(s) 94
PctI GAATGC 1 cut(s) 204
PfeI GAWTC 1 cut(s) 67
PkrI GCNGC 2 cut(s) 92, 325
PleI GAGTC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 255
PspN4I GGNNCC 5 cut(s) 44, 82, 233, 261, 316
PspPI GGNCC 3 cut(s) 73, 141, 260
PstNI CAGNNNCTG 1 cut(s) 31
PsuI RGATCY 1 cut(s) 231
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 2 cut(s) 91, 324
Sau3AI GATC 2 cut(s) 55, 231
Sau96I GGNCC 3 cut(s) 73, 141, 260
SchI GAGTC 1 cut(s) 256
ScrFI CCNGG 1 cut(s) 72
SetI ASST 8 cut(s) 18, 33, 48, 190, 221, 243, 337, 389
SfaNI GCATC 1 cut(s) 154
Sfr274I CTCGAG 1 cut(s) 9
SinI GGWCC 2 cut(s) 141, 260
SlaI CTCGAG 1 cut(s) 9
SmlI CTYRAG 2 cut(s) 9, 388
SmoI CTYRAG 2 cut(s) 9, 388
SsiI CCGC 3 cut(s) 39, 118, 451
StyD4I CCNGG 1 cut(s) 70
TaaI ACNGT 2 cut(s) 306, 367
TaqI TCGA 3 cut(s) 10, 114, 432
TfiI GAWTC 1 cut(s) 67
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 3 cut(s) 175, 303, 325
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 2 cut(s) 90, 323
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 1 cut(s) 80
TspRI CASTG 3 cut(s) 175, 303, 325
VpaK11BI GGWCC 2 cut(s) 141, 260
XhoI CTCGAG 1 cut(s) 9
XmiI GTMKAC 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.