Rh3AG221800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0003219
Physical Location & Seq
Forward (+)
22721830 .. 22762140
40311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG221800.1

Sequence Viewer

Length: 375 bp
ATGGGGACGAGAGAGGTGTATGAACAGAAGCTAAGGAGTGGGAATCTGCATCATGATCCTACCATGAAACCAGGCCTCGGCACCCCTCGCTGCCCGCGCTGTCTCTCTCTCCTTGACCATGATTCGGGAAAGGGTGAATGGACTATCACTCCTGTTCTTCATGACGCCACTGCTGTTGCTGGCTCAGGTATTGGTGGAATGCTTAGTGCAATTCATGGCTTCAATACAGGGATCCCGTACCTTCAGAATCGCTTAAAAGGACCAAAGTGGCTTCCTTTCTTAGTTGGGCTTCCTCCTCTGCTACTCTTTTCGGGTGCCAGTGCTGCATTTGGAGGTATGAACCTTGCCCTTTCTCCATGGGTTAAAGTACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

124

Amino Acids

13.23

Weight (kDa)

9.39

Isoelectric Point (pI)

20.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 80, 314
AccII CGCG 1 cut(s) 97
AciI CCGC 1 cut(s) 95
AclWI GGATC 3 cut(s) 50, 226, 239
AcuI CTGAAG 1 cut(s) 227
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 2 cut(s) 239, 369
AfiI CCNNNNNNNGG 2 cut(s) 77, 124
AgsI TTSAA 1 cut(s) 223
AjnI CCWGG 1 cut(s) 70
AluBI AGCT 1 cut(s) 31
AluI AGCT 1 cut(s) 31
Alw26I GTCTC 1 cut(s) 107
AlwI GGATC 3 cut(s) 50, 226, 239
AoxI GGCC 1 cut(s) 73
ApeKI GCWGC 2 cut(s) 90, 323
AspLEI GCGC 1 cut(s) 99
AspS9I GGNCC 1 cut(s) 260
AsuHPI GGTGA 1 cut(s) 146
AvaII GGWCC 1 cut(s) 260
BamHI GGATCC 1 cut(s) 231
BanI GGYRCC 2 cut(s) 80, 314
BbvI GCAGC 2 cut(s) 77, 310
BciT130I CCWGG 1 cut(s) 72
BcoDI GTCTC 1 cut(s) 107
BisI GCNGC 2 cut(s) 91, 324
BlsI GCNGC 2 cut(s) 92, 325
BmcAI AGTACT 1 cut(s) 369
Bme1390I CCNGG 1 cut(s) 72
Bme18I GGWCC 1 cut(s) 260
BmgT120I GGNCC 1 cut(s) 260
BmiI GGNNCC 3 cut(s) 82, 233, 316
BmrFI CCNGG 1 cut(s) 72
BmsI GCATC 1 cut(s) 58
Bpu10I CCTNAGC 2 cut(s) 32, 184
BsaHI GRCGYC 1 cut(s) 165
BsaJI CCNNGG 2 cut(s) 76, 356
BsaXI ACNNNNNCTCC 2 cut(s) 133, 163
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 124
Bse1I ACTGG 1 cut(s) 318
BseBI CCWGG 1 cut(s) 72
BseDI CCNNGG 2 cut(s) 76, 356
BseLI CCNNNNNNNGG 2 cut(s) 77, 124
BseMII CTCAG 1 cut(s) 198
BseNI ACTGG 1 cut(s) 318
BseRI GAGGAG 1 cut(s) 285
BseXI GCAGC 2 cut(s) 77, 310
Bsh1236I CGCG 1 cut(s) 97
BshFI GGCC 1 cut(s) 75
BshNI GGYRCC 2 cut(s) 80, 314
BslFI GGGAC 1 cut(s) 19
BslI CCNNNNNNNGG 2 cut(s) 77, 124
BsmAI GTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 19
BsmI GAATGC 1 cut(s) 204
BsnI GGCC 1 cut(s) 75
Bsp143I GATC 2 cut(s) 55, 231
Bsp19I CCATGG 1 cut(s) 356
BspACI CCGC 1 cut(s) 95
BspANI GGCC 1 cut(s) 75
BspCNI CTCAG 1 cut(s) 197
BspFNI CGCG 1 cut(s) 97
BspHI TCATGA 2 cut(s) 52, 160
BspLI GGNNCC 3 cut(s) 82, 233, 316
BspPI GGATC 3 cut(s) 50, 226, 239
BspT107I GGYRCC 2 cut(s) 80, 314
BsrI ACTGG 1 cut(s) 318
BssECI CCNNGG 2 cut(s) 76, 356
BssMI GATC 2 cut(s) 55, 231
BssNI GRCGYC 1 cut(s) 165
BssT1I CCWWGG 1 cut(s) 356
Bst2UI CCWGG 1 cut(s) 72
BstACI GRCGYC 1 cut(s) 165
BstC8I GCNNGC 2 cut(s) 95, 181
BstDEI CTNAG 4 cut(s) 32, 184, 203, 280
BstDSI CCRYGG 1 cut(s) 356
BstFNI CGCG 1 cut(s) 97
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 2 cut(s) 58, 234
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 2 cut(s) 55, 231
BstMWI GCNNNNNNNGC 3 cut(s) 87, 96, 323
BstNI CCWGG 1 cut(s) 72
BstSCI CCNGG 1 cut(s) 70
BstUI CGCG 1 cut(s) 97
BstV1I GCAGC 2 cut(s) 77, 310
BstX2I RGATCY 1 cut(s) 231
BstYI RGATCY 1 cut(s) 231
BsuRI GGCC 1 cut(s) 75
BtgI CCRYGG 1 cut(s) 356
BtsI GCAGTG 1 cut(s) 168
BtsIMutI CAGTG 2 cut(s) 168, 325
Cac8I GCNNGC 2 cut(s) 95, 181
CciI TCATGA 2 cut(s) 52, 160
CfoI GCGC 1 cut(s) 99
Cfr13I GGNCC 1 cut(s) 260
CseI GACGC 1 cut(s) 173
Csp6I GTAC 2 cut(s) 238, 368
CspCI CAANNNNNGTGG 2 cut(s) 157, 192
CviAII CATG 6 cut(s) 53, 64, 119, 161, 215, 357
CviJI RGCY 6 cut(s) 31, 75, 183, 219, 271, 289
CviKI_1 RGCY 6 cut(s) 31, 75, 183, 219, 271, 289
CviQI GTAC 2 cut(s) 238, 368
DdeI CTNAG 4 cut(s) 32, 184, 203, 280
DpnI GATC 2 cut(s) 57, 233
DpnII GATC 2 cut(s) 55, 231
Eco130I CCWWGG 1 cut(s) 356
Eco147I AGGCCT 1 cut(s) 75
Eco47I GGWCC 1 cut(s) 260
Eco57I CTGAAG 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 70
EcoT14I CCWWGG 1 cut(s) 356
ErhI CCWWGG 1 cut(s) 356
FaeI CATG 6 cut(s) 56, 67, 122, 164, 218, 360
FaiI YATR 8 cut(s) 21, 54, 65, 120, 162, 216, 338, 358
FaqI GGGAC 1 cut(s) 19
FatI CATG 6 cut(s) 52, 63, 118, 160, 214, 356
FauI CCCGC 1 cut(s) 102
Fnu4HI GCNGC 2 cut(s) 91, 324
Fsp4HI GCNGC 2 cut(s) 91, 324
GlaI GCGC 1 cut(s) 98
GluI GCNGC 2 cut(s) 91, 324
HaeIII GGCC 1 cut(s) 75
HgaI GACGC 1 cut(s) 173
HhaI GCGC 1 cut(s) 99
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 6 cut(s) 56, 67, 122, 164, 218, 360
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HinfI GANTC 3 cut(s) 43, 122, 247
HphI GGTGA 1 cut(s) 146
Hpy188I TCNGA 2 cut(s) 246, 374
Hpy188III TCNNGA 3 cut(s) 53, 126, 161
HpyAV CCTTC 1 cut(s) 251
HpyCH4V TGCA 3 cut(s) 49, 209, 326
HpyF10VI GCNNNNNNNGC 3 cut(s) 87, 96, 323
HpyF3I CTNAG 4 cut(s) 32, 184, 203, 280
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 6 cut(s) 56, 67, 122, 164, 218, 360
HspAI GCGC 1 cut(s) 97
Kzo9I GATC 2 cut(s) 55, 231
LpnPI CCDG 7 cut(s) 57, 84, 165, 165, 171, 213, 331
Lsp1109I GCAGC 2 cut(s) 77, 310
LweI GCATC 1 cut(s) 58
MalI GATC 2 cut(s) 57, 233
MboI GATC 2 cut(s) 55, 231
MboII GAAGA 1 cut(s) 149
MflI RGATCY 1 cut(s) 231
MluCI AATT 1 cut(s) 210
MnlI CCTC 6 cut(s) 7, 86, 96, 303, 306, 326
MseI TTAA 2 cut(s) 254, 363
MspR9I CCNGG 1 cut(s) 72
Mva1269I GAATGC 1 cut(s) 204
MvaI CCWGG 1 cut(s) 72
MvnI CGCG 1 cut(s) 97
MwoI GCNNNNNNNGC 3 cut(s) 87, 96, 323
NcoI CCATGG 1 cut(s) 356
NdeII GATC 2 cut(s) 55, 231
NlaIII CATG 6 cut(s) 56, 67, 122, 164, 218, 360
NlaIV GGNNCC 3 cut(s) 82, 233, 316
NmeAIII GCCGAG 1 cut(s) 57
PagI TCATGA 2 cut(s) 52, 160
PceI AGGCCT 1 cut(s) 75
PctI GAATGC 1 cut(s) 204
PfeI GAWTC 3 cut(s) 43, 122, 247
PkrI GCNGC 2 cut(s) 92, 325
Psp6I CCWGG 1 cut(s) 70
PspGI CCWGG 1 cut(s) 70
PspN4I GGNNCC 3 cut(s) 82, 233, 316
PspPI GGNCC 1 cut(s) 260
PsuI RGATCY 1 cut(s) 231
RsaI GTAC 2 cut(s) 239, 369
RsaNI GTAC 2 cut(s) 238, 368
SaqAI TTAA 2 cut(s) 254, 363
SatI GCNGC 2 cut(s) 91, 324
Sau3AI GATC 2 cut(s) 55, 231
Sau96I GGNCC 1 cut(s) 260
ScaI AGTACT 1 cut(s) 369
ScrFI CCNGG 1 cut(s) 72
SetI ASST 6 cut(s) 18, 33, 190, 243, 337, 345
SfaNI GCATC 1 cut(s) 58
SinI GGWCC 1 cut(s) 260
Sse9I AATT 1 cut(s) 210
SseBI AGGCCT 1 cut(s) 75
SsiI CCGC 1 cut(s) 95
StuI AGGCCT 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 70
StyI CCWWGG 1 cut(s) 356
TasI AATT 1 cut(s) 210
TatI WGTACW 1 cut(s) 367
TfiI GAWTC 3 cut(s) 43, 122, 247
Tru1I TTAA 2 cut(s) 254, 363
Tru9I TTAA 2 cut(s) 254, 363
TscAI CASTG 2 cut(s) 175, 325
TseI GCWGC 2 cut(s) 90, 323
TspDTI ATGAA 5 cut(s) 36, 80, 149, 203, 353
TspRI CASTG 2 cut(s) 175, 325
VpaK11BI GGWCC 1 cut(s) 260
ZrmI AGTACT 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.