Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0004317

Physical Location & Seq

Genomic Coordinates Reverse (-)

36526518 .. 36530960

Gene Structure 4443 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1285700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4113 bp

ATGGAGTCCAAGCTTTTTGCTTCAGAGGAGGAGGCGCAGCACCTCTCAAACGCACCAGGTTCTTCATTTCTTTCTTCTCAGTACTCTGTTATCGATGATTCATCGAAAACCCATGTTTCCAAAATTGATAATGACGAGAAATTTAGCGGGGTTGGTGGCGGTAGTGACGGTGGTGGGTCCGAGACGGAGGAGGGGTTTGTAAGCGGGGAGGAGGAGGATTTTGATTCCGAGAGGGCGTTTGTGAGAGGCGGAGATACCGTGGTGGTTGGAGGGGTGCAGGAGGATTCCGGTGTGAAGTTTGTTAACCCGTCGGAATTCTTCTATCCGACGAGTACGAGTGGTCTGCGGCCGATTGCCAAGGTATCGGTGGATGATGACGACGGCGAGGAGGATGCTGGGGAGGAAGTGTTTATGGATTCGAAGAGTTTAGAGGGTGGGAGTCCTAAAGGTGTTGTTTTGGGGGATAGTGGGTTGGGGAAGAGTGAAAATTTGGGTGAAGTGGTCAAGGAAAATGGTGTTAGTGGTGAAGAAGAAGATAAGGTAGTTGAGAATTCGAAATTGGTAGAGGGTTTGGGCTCGGTGGTGGAGAAGCCGGTGGAGGTGGCTGAAGTAGATGATAAGAAGGCTGTAGAGCAGCCGAAGGAGGAGCATGCTTTCAATGGCGAAACGGAAGTTAGTAAAGAAAATGTTGTGGCGGTGGATGCGCCAGCTGATGATAAGTTGGTTGAGGTTGAAGAGAATGGTGTGGAAATTACTAGTGGAGGTGACTCAGTTGTGCAGGATATACATGCCAATTTGTCACAGACAGGAGCAGCTGTTGTGGTTGGCGATGTAGAGACGATCGAGGAATCTGAAATAAAAGGCTTGGAGGTTGATGAAGGTGTGAGTTTGGATAATGGTTTTGATCAAATTAGCTCTGAGACAGAAGAACCAAATGACTCGGAGTCCGTGGCTGTGGATTCTGAGCGTGACAGAGCGGTTGTTCCCAAGTCTGGGAATGTAGAACTTGATGGAGAGAAAGCTGCTATTGTTGCTGGTGCTGATGAAGTTGATCTTGAGAAGAGGCCAGAAAGGAAGGATGAACTGAAATCTAATTCCGAAACCAGAGAGAAAGGGCTGGCAACGGAATCAGGTGCTGATGTGATGTCAGATGACAAACCATCGGTAGGGGATGGAGCTGAAACAGAAACTGTAAACTTATCAGAACATGAACCGCAGATAGAGGCAAAACCGGAGAACGGTGACTTGAATGCACATGATGAAGTGCATGAATTGGAAGCGGCTGTCTGCGGAAAATATGTGGCACCTGAATTTCTGGATTCAAGTTCTGATAGCCACAAAGTAAAGCTAGTTGAGGAAGTTGAGGAGAAGCATATCCCAGATGAAATTGGCATTGGATCTGTGGATGCAAATTCCATTTCAAACAGAGAGATAAGAGTTGAAACTGACGATGACGGTGATAATGATCTTCAATATGATGATAAGGATCTTCAAGACGATGAAGGTGAGAATGAAGGTTCAGTTACAGATGCGAACAATGAAGGTATGATTTTTGGAAGCTCTGAGGCTGCTAAACAGTTTTTGGAACAGTTGGAGCGAGGATCCCGTGCTGGTTCTTACTCAGGTGCTGACAGTTATCATGATCATTCACAGAGAATTGATGGCCAGGTTGCCACGGATTCAGATGAAGAAGTGGACACTGATGAGGAAGGGGGCGGAAAAGAGTTATTTGATTCTGATGCACTGGCAGCGCTTCTGAAAGCAGCAACAGGTGCTTCCTCAGATGGTGGCAATGTTACATTCACCACCCCAGATGGATCCAGGCTTTTCTCTGTTGAGCGTCCTGCTGGGTTGGGATCATCAGTCCGGTCGTTGAAACCTGCTTCCCGACCAAACAACTCGAACCTTTTTTCATCTTCCAATGTCACAGTTGGGGGCAGTTCTGAGAACTTGAGTGAAGAAGAGAAAGCGAAGCTCGAAAAGTTTCAGCAAATAAGGGTTAAGTTCTTGAGGCTCGTGCAGAGATTAGGTGTTTCTACAGAAGAGTCAGTACCAAGGCAGGTTCTATACCGCCTAGCTCTTGTGTCAGGGAGGCAAAACAGTCAAGAATTTAGCCTTGAAGCTGCAAAGATGACCGCTCTTCAGCTTGAAGCAGAGGAGAAGGATGATTTGGACTTCTCCTTGAACATACTGGTTCTTGGGAAGACAGGTGTTGGGAAAAGTGCCACTATAAATTCGATTTTCGGTGCAGAAGTGACCCCAATTAATGCTTTTGGACCTGGAACAACTGCTGTGAAAGAAATTGTTGGAGTGGTGGATGGAGTCAAGATTAAGGTTTTTGATACACCGGGTCTGAAATCTGCTGCAATGGAACAGGGCATCAATCGCAAAATCTTATCTTCTGTGCAGAGGCGCATGAAAAAATCCCCCCCAGATATTGTCCTCTATGTGGATCGGATGGACTCCCGAAGTAGGGATCTAAGTGATGTGCCATTGTTAAGATCAATCACTAATGCCTTTGGTCCTTCAATTTGGCGAAGCACCATAGTTACTCTGACTCACAGCTCTTCTGCTCCTCCTGATGGACCATCAGGTTCCCCTCTGAATTATGAGATGTTTCGTAATCAGCGGGAACAAATAATGCAGCAGACCATTGGACAAGCTGTTGGCGATCTAAGGTTCATGAATCCGAGTATGATGAGTCCAATATCTCTTGTAGAGAACCACCCATCTTGTCGGAAGAGAGATAATCAGAAAGTTCTCCCTAATGGTGTAGCTTGGAGATCCCATCTATTGCTCTTATGCTACTCTATGAAGATCTTGTCTGATGCGTCTAATCTCTCCAAACCTCCGGAATCATTTGATCACCGTAAGCTCTTTGGTTTGCGTTCCCGTTCGGCTCCTCTTCCATACTTATTGTCTTGGCTGTTGCAATCTCGTCCGCACCCAAAACTTGCTACCGATCAAGGTGGTGAGAATGCTGATTCAGACATTGACTTGGCTGACTTGTCTGATTCTGATCAAGAGGAAGAGGAGGATGAGTATGATCAGCTTCCGTCATTTAAGCCTCTCAAGAAGGCTCAGATTGCTAAGCTTAGCAGAGAGCAGAGGAAGGCGTACGCTGAGGAGTACGATTATAGGTTTAAGCTCCTCCAGAAGAAGATGTGGAAAGAGGAGTTGAGAAGGATGAAAGAAATGAAGAAGAAGGGCAAGGTCAGTGAAGAGGAGTACGGTTATTTGGGTGAAGAAGATCCAGAAAATGGTGCTCCAGCAGCTGTGCCGGTGGCATTACCTGATATGGTTTTGCCGCCTTCTTTTGATGGTGAAAATCCAGCTTACAGGTACCGGTTCTTGGAGCCGACTTCTCAGTTCACGGCAAGGCCAGTGTTGGACGCGCAAGGCTGGGACCATGACTGTGGGTATGACGGTGTCAACCTTGAACATACTCTGGCCATTGCTAATTCTTTTCCCGCAGCTGTTGCTGTTCAGCTTACAAAGGATAAGAAAGAGTTCAATATTCATTTAGATTCCTCGGTTGGTGCTAAGCACGGGGAGAAGTGGTCGAGTATGCTAGGGTTTGACATTCAGAACATCGGAAAGCAACTTGCATACATTGTCCGAGGAGACACCAAAGTCAAAACTTCCAAGAGGTTCAAGAATGCTGCCGGAGTATCTGTGACATTCTTGGGTGAAAATGTGTCCACTGGATTCAAACTCGAACAGGAGTATGCGGTTGGCAAGCGCCTGGTATTGGTGGGTACTACTGGTACTGTCCGATCTCAGGGCGACTCAGCATATGGAGCAAATTTGGAGGTGCGGCTGAGGGAGGCAGATTTTCCGATTGGCCAGGATCAATCCTCATTGGGTCTGTCTCTCGTGCGGTGGAGGGGTGACTTGGCCCTGGGGGCAAATCTCCAGTCCCAGTTTTCCCTCGGCCGCAACTACAAGATGGCTGTTCGTGCAGGGTTGAACAACAAGCGCAGCGGACAGATCTCGATCAGAACAAGCAGCTCCGAACAACTCCAACTTGCGCTCATTGCCGTGATTCCGGTTGTCAAGGCTATCTACAACAGCATCTGGCCCAGAGCCAGCGAGAACTACTCCATCTACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003924 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006605 GO:0006810 GO:0006886 GO:0007154 GO:0007165 GO:0007186 GO:0008104 GO:0008150 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009941 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017038 GO:0017111 GO:0019867 GO:0023052 GO:0031090 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0038023 GO:0042170 GO:0042886 GO:0043021 GO:0043024 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045036 GO:0045037 GO:0045184 GO:0046907 GO:0050789 GO:0050794 GO:0050896 GO:0051087 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055085 GO:0060089 GO:0061927 GO:0065002 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0072594 GO:0072596 GO:0072598 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AIG1 TOC159_MAD

Protein Analysis

1371

Amino Acids

148.78

Weight (kDa)

4.62

Isoelectric Point (pI)

46.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AIG1	PF04548	728 - 862	2.9e-20	AIG1 family
MMR_HSR1	PF01926	730 - 817	3.4e-09	50S ribosome-binding GTPase
TOC159_MAD	PF11886	1093 - 1357	7.4e-122	Translocase of chloroplast 159/132, membrane anchor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004317)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02482 AT4G02510 AT4G02510 AT4G02510 AT4G02510
fragaria_vesca	FvH4_2g25480
malus_domestica	MD03G1285700.v1.1 MD11G1305100.v1.1
prunus_persica	Prupe.8G258800_v2.0.a1 Prupe.8G258800_v2.0.a1
pyrus_communis	pycom03g22640 pycom11g26690
rosa_chinensis	RchiOBHm_Chr6g0293691
rosa_laevigata	RLG00000011932
rosa_multiflora	Rmu_co8307375.1_g000001 Rmu_co8497971.1_g000001 Rmu_sc0005431.1_g000009
rosa_roxburghii	Rroxscaffold_7G00173980
rosa_rugosa	Rorug06G0237500 Rorug06G0237500
rosa_samantha	Rh4AG062400 Rh4AG063400 Rh4AG067300 Rh4BG061800 Rh4CG068100 Rh4DG056700 Rh6AG350100 Rh6BG357600 Rh6CG364400 Rh6DG351300
rosa_wichuraiana	Rw0G022990 Rw6G030480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	3632
Acc36I	ACCTGC	2 cut(s)	1888, 2050
Acc65I	GGTACC	1 cut(s)	3342
AccB1I	GGYRCC	2 cut(s)	1303, 3342
AccB7I	CCANNNNNTGG	1 cut(s)	3260
AccBSI	CCGCTC	2 cut(s)	977, 2138
AccII	CGCG	1 cut(s)	3395
AccIII	TCCGGA	1 cut(s)	2851
AcoI	YGGCCR	5 cut(s)	347, 1663, 3450, 3844, 3934
AcsI	RAATTY	9 cut(s)	140, 314, 487, 550, 1310, 1411, 2108, 2233, 3805
AcuI	CTGAAG	3 cut(s)	6, 627, 2126
AfaI	GTAC	9 cut(s)	83, 334, 2052, 3119, 3131, 3230, 3344, 3760, 3769
AfeI	AGCGCT	1 cut(s)	1752
AgeI	ACCGGT	1 cut(s)	3345
AhdI	GACNNNNNGTC	1 cut(s)	943
AhlI	ACTAGT	1 cut(s)	755
AjnI	CCWGG	7 cut(s)	55, 1665, 1820, 2278, 3744, 3846, 3900
AjuI	GAANNNNNNNTTGG	6 cut(s)	542, 574, 1377, 1409, 2153, 2185
Alw21I	GWGCWC	1 cut(s)	3268
Alw26I	GTCTC	5 cut(s)	176, 830, 914, 3618, 3876
AlwNI	CAGNNNCTG	5 cut(s)	1136, 1190, 1628, 3275, 4077
Aor13HI	TCCGGA	1 cut(s)	2851
Aor51HI	AGCGCT	1 cut(s)	1752
AoxI	GGCC	9 cut(s)	347, 1064, 1663, 3380, 3450, 3844, 3897, 3934, 4079
ApoI	RAATTY	9 cut(s)	140, 314, 487, 550, 1310, 1411, 2108, 2233, 3805
ArsI	GACNNNNNNTTYG	2 cut(s)	3902, 3934
AseI	ATTAAT	1 cut(s)	2265
AsiGI	ACCGGT	1 cut(s)	3345
Asp700I	GAANNNNTTC	2 cut(s)	1983, 3509
Asp718I	GGTACC	1 cut(s)	3342
AspLEI	GCGC	8 cut(s)	37, 706, 1753, 2415, 3397, 3744, 3981, 4033
AspS9I	GGNCC	7 cut(s)	177, 2276, 2522, 2585, 3406, 3898, 4080
AsuC2I	CCSGG	1 cut(s)	2349
AsuII	TTCGAA	2 cut(s)	419, 554
AvaII	GGWCC	5 cut(s)	177, 2276, 2522, 2585, 3406
BaeI	ACNNNNGTAYC	2 cut(s)	246, 279
BalI	TGGCCA	3 cut(s)	1665, 3452, 3846
BamHI	GGATCC	2 cut(s)	1601, 1817
BanI	GGYRCC	2 cut(s)	1303, 3342
BanII	GRGCYC	1 cut(s)	578
BarI	GAAGNNNNNNTAC	2 cut(s)	2034, 2066
BauI	CACGAG	2 cut(s)	2015, 3875
BbsI	GAAGAC	1 cut(s)	2210
Bbv12I	GWGCWC	1 cut(s)	3268
BbvCI	CCTCAGC	2 cut(s)	3123, 3821
BceAI	ACGGC	3 cut(s)	397, 3390, 4025
BcgI	CGANNNNNNTGC	4 cut(s)	325, 359, 374, 408
BciT130I	CCWGG	7 cut(s)	57, 1667, 1822, 2280, 3746, 3848, 3902
BclI	TGATCA	5 cut(s)	904, 1642, 2863, 3019, 3046
BcnI	CCSGG	1 cut(s)	2349
BcoDI	GTCTC	5 cut(s)	176, 830, 914, 3618, 3876
BcuI	ACTAGT	1 cut(s)	755
BfaI	CTAG	4 cut(s)	756, 1349, 2075, 3572
BfmI	CTRYAG	2 cut(s)	627, 2037
BfoI	RGCGCY	2 cut(s)	1754, 3745
BfuAI	ACCTGC	2 cut(s)	1888, 2050
BglI	GCCNNNNNGGC	1 cut(s)	3905
BglII	AGATCT	2 cut(s)	2817, 3990
BlpI	GCTNAGC	3 cut(s)	3090, 3095, 3543
BmcAI	AGTACT	1 cut(s)	83
Bme1390I	CCNGG	8 cut(s)	57, 1667, 1822, 2280, 2349, 3746, 3848, 3902
Bme18I	GGWCC	5 cut(s)	177, 2276, 2522, 2585, 3406
BmeRI	GACNNNNNGTC	1 cut(s)	943
BmgT120I	GGNCC	7 cut(s)	177, 2276, 2522, 2585, 3406, 3898, 4080
BmiI	GGNNCC	9 cut(s)	178, 1305, 1603, 1819, 2596, 2901, 3344, 3357, 3407
BmrFI	CCNGG	8 cut(s)	57, 1667, 1822, 2280, 2349, 3746, 3848, 3902
BmrI	ACTGGG	1 cut(s)	3916
BmsI	GCATC	8 cut(s)	382, 691, 1396, 1519, 1729, 2388, 2818, 4083
BmuI	ACTGGG	1 cut(s)	3916
BpiI	GAAGAC	1 cut(s)	2210
BplI	GAGNNNNNCTC	1 cut(s)	4085
BpmI	CTGGAG	3 cut(s)	3137, 3252, 3899
Bpu10I	CCTNAGC	2 cut(s)	3123, 3821
Bpu1102I	GCTNAGC	3 cut(s)	3090, 3095, 3543
Bpu14I	TTCGAA	2 cut(s)	419, 554
BpuEI	CTTGAG	4 cut(s)	1076, 1972, 2029, 3056
BpuMI	CCSGG	1 cut(s)	2349
Bsa29I	ATCGAT	1 cut(s)	93
BsaBI	GATNNNNATC	4 cut(s)	1464, 1485, 3018, 3995
BsaWI	WCCGGW	6 cut(s)	287, 1231, 1866, 2851, 3345, 4048
Bse118I	RCCGGY	3 cut(s)	592, 3280, 3345
Bse1I	ACTGG	7 cut(s)	1749, 2196, 3383, 3709, 3769, 3916, 3922
Bse3DI	GCAATG	4 cut(s)	1798, 2373, 3453, 4035
Bse8I	GATNNNNATC	4 cut(s)	1464, 1485, 3018, 3995
BseAI	TCCGGA	1 cut(s)	2851
BseBI	CCWGG	7 cut(s)	57, 1667, 1822, 2280, 3746, 3848, 3902
BseCI	ATCGAT	1 cut(s)	93
BseJI	GATNNNNATC	4 cut(s)	1464, 1485, 3018, 3995
BseMI	GCAATG	4 cut(s)	1798, 2373, 3453, 4035
BseNI	ACTGG	7 cut(s)	1749, 2196, 3383, 3709, 3769, 3916, 3922
BseX3I	CGGCCG	2 cut(s)	347, 3934
BseYI	CCCAGC	3 cut(s)	395, 1847, 3402
BsgI	GTGCAG	6 cut(s)	296, 797, 2039, 2268, 2426, 3981
Bsh1236I	CGCG	1 cut(s)	3395
Bsh1285I	CGRYCG	4 cut(s)	350, 843, 1871, 3937
BshFI	GGCC	9 cut(s)	349, 1066, 1665, 3382, 3452, 3846, 3899, 3936, 4081
BshNI	GGYRCC	2 cut(s)	1303, 3342
BshTI	ACCGGT	1 cut(s)	3345
BshVI	ATCGAT	1 cut(s)	93
BsiEI	CGRYCG	4 cut(s)	350, 843, 1871, 3937
BsiHKAI	GWGCWC	1 cut(s)	3268
BsiWI	CGTACG	1 cut(s)	3117
BslFI	GGGAC	2 cut(s)	3419, 3904
BsmAI	GTCTC	5 cut(s)	176, 830, 914, 3618, 3876
BsmBI	CGTCTC	2 cut(s)	176, 830
BsmFI	GGGAC	2 cut(s)	3419, 3904
BsmI	GAATGC	3 cut(s)	1255, 2983, 3664
BsnI	GGCC	9 cut(s)	349, 1066, 1665, 3382, 3452, 3846, 3899, 3936, 4081
Bsp119I	TTCGAA	2 cut(s)	419, 554
Bsp1286I	GDGCHC	2 cut(s)	578, 3268
Bsp13I	TCCGGA	1 cut(s)	2851
Bsp1720I	GCTNAGC	3 cut(s)	3090, 3095, 3543
BspANI	GGCC	9 cut(s)	349, 1066, 1665, 3382, 3452, 3846, 3899, 3936, 4081
BspDI	ATCGAT	1 cut(s)	93
BspEI	TCCGGA	1 cut(s)	2851
BspFNI	CGCG	1 cut(s)	3395
BspHI	TCATGA	2 cut(s)	1639, 2682
BspLI	GGNNCC	9 cut(s)	178, 1305, 1603, 1819, 2596, 2901, 3344, 3357, 3407
BspMI	ACCTGC	2 cut(s)	1888, 2050
BspQI	GCTCTTC	2 cut(s)	2145, 2572
BspT104I	TTCGAA	2 cut(s)	419, 554
BspT107I	GGYRCC	2 cut(s)	1303, 3342
BsrBI	CCGCTC	2 cut(s)	977, 2138
BsrDI	GCAATG	4 cut(s)	1798, 2373, 3453, 4035
BsrFI	RCCGGY	3 cut(s)	592, 3280, 3345
BsrI	ACTGG	7 cut(s)	1749, 2196, 3383, 3709, 3769, 3916, 3922
BssAI	RCCGGY	3 cut(s)	592, 3280, 3345
BssSI	CACGAG	2 cut(s)	2015, 3875
BssT1I	CCWWGG	2 cut(s)	357, 2054
Bst2BI	CACGAG	2 cut(s)	2015, 3875
Bst2UI	CCWGG	7 cut(s)	57, 1667, 1822, 2280, 3746, 3848, 3902
BstAPI	GCANNNNNTGC	2 cut(s)	1772, 3479
BstBI	TTCGAA	2 cut(s)	419, 554
BstC8I	GCNNGC	5 cut(s)	651, 708, 1119, 3740, 4090
BstDSI	CCRYGG	3 cut(s)	258, 948, 1674
BstFNI	CGCG	1 cut(s)	3395
BstH2I	RGCGCY	2 cut(s)	1754, 3745
BstHHI	GCGC	8 cut(s)	37, 706, 1753, 2415, 3397, 3744, 3981, 4033
BstMAI	GTCTC	5 cut(s)	176, 830, 914, 3618, 3876
BstMCI	CGRYCG	4 cut(s)	350, 843, 1871, 3937
BstNI	CCWGG	7 cut(s)	57, 1667, 1822, 2280, 3746, 3848, 3902
BstNSI	RCATGY	2 cut(s)	653, 791
BstSCI	CCNGG	8 cut(s)	55, 1665, 1820, 2278, 2347, 3744, 3846, 3900
BstSFI	CTRYAG	2 cut(s)	627, 2037
BstUI	CGCG	1 cut(s)	3395
BstV2I	GAAGAC	1 cut(s)	2210
BstX2I	RGATCY	9 cut(s)	1397, 1486, 1601, 1817, 2476, 2783, 2817, 3250, 3990
BstXI	CCANNNNNNTGG	1 cut(s)	3416
BstYI	RGATCY	9 cut(s)	1397, 1486, 1601, 1817, 2476, 2783, 2817, 3250, 3990
BstZI	CGGCCG	2 cut(s)	347, 3934
Bsu15I	ATCGAT	1 cut(s)	93
BsuRI	GGCC	9 cut(s)	349, 1066, 1665, 3382, 3452, 3846, 3899, 3936, 4081
BsuTUI	ATCGAT	1 cut(s)	93
BtgI	CCRYGG	3 cut(s)	258, 948, 1674
BtgZI	GCGATG	1 cut(s)	843
BtsIMutI	CAGTG	5 cut(s)	1698, 1742, 3223, 3390, 3702
BveI	ACCTGC	2 cut(s)	1888, 2050
Cac8I	GCNNGC	5 cut(s)	651, 708, 1119, 3740, 4090
CaiI	CAGNNNCTG	5 cut(s)	1136, 1190, 1628, 3275, 4077
CciI	TCATGA	2 cut(s)	1639, 2682
CfoI	GCGC	8 cut(s)	37, 706, 1753, 2415, 3397, 3744, 3981, 4033
Cfr10I	RCCGGY	3 cut(s)	592, 3280, 3345
Cfr13I	GGNCC	7 cut(s)	177, 2276, 2522, 2585, 3406, 3898, 4080
ClaI	ATCGAT	1 cut(s)	93
CseI	GACGC	3 cut(s)	1829, 2820, 3401
CsiI	ACCWGGT	1 cut(s)	55
Csp6I	GTAC	9 cut(s)	82, 333, 2051, 3118, 3130, 3229, 3343, 3759, 3768
CspAI	ACCGGT	1 cut(s)	3345
CspCI	CAANNNNNGTGG	2 cut(s)	2714, 2749
CviQI	GTAC	9 cut(s)	82, 333, 2051, 3118, 3130, 3229, 3343, 3759, 3768
DrdI	GACNNNNNNGTC	1 cut(s)	3632
DriI	GACNNNNNGTC	1 cut(s)	943
DseDI	GACNNNNNNGTC	1 cut(s)	3632
EaeI	YGGCCR	5 cut(s)	347, 1663, 3450, 3844, 3934
EagI	CGGCCG	2 cut(s)	347, 3934
Eam1105I	GACNNNNNGTC	1 cut(s)	943
EciI	GGCGGA	2 cut(s)	264, 1731
EclXI	CGGCCG	2 cut(s)	347, 3934
Eco130I	CCWWGG	2 cut(s)	357, 2054
Eco24I	GRGCYC	1 cut(s)	578
Eco47I	GGWCC	5 cut(s)	177, 2276, 2522, 2585, 3406
Eco47III	AGCGCT	1 cut(s)	1752
Eco52I	CGGCCG	2 cut(s)	347, 3934
Eco57I	CTGAAG	3 cut(s)	6, 627, 2126
EcoRI	GAATTC	2 cut(s)	314, 550
EcoRII	CCWGG	7 cut(s)	55, 1665, 1820, 2278, 3744, 3846, 3900
EcoT14I	CCWWGG	2 cut(s)	357, 2054
EcoT38I	GRGCYC	1 cut(s)	578
ErhI	CCWWGG	2 cut(s)	357, 2054
Esp3I	CGTCTC	2 cut(s)	176, 830
FalI	AAGNNNNNCTT	2 cut(s)	1038, 1070
FaqI	GGGAC	2 cut(s)	3419, 3904
FauI	CCCGC	4 cut(s)	140, 197, 2622, 3478
FauNDI	CATATG	1 cut(s)	3796
FbaI	TGATCA	5 cut(s)	904, 1642, 2863, 3019, 3046
FriOI	GRGCYC	1 cut(s)	578
FspBI	CTAG	4 cut(s)	756, 1349, 2075, 3572
GlaI	GCGC	8 cut(s)	36, 705, 1752, 2414, 3396, 3743, 3980, 4032
GsaI	CCCAGC	3 cut(s)	399, 1851, 3406
GsuI	CTGGAG	3 cut(s)	3137, 3252, 3899
HaeII	RGCGCY	2 cut(s)	1754, 3745
HaeIII	GGCC	9 cut(s)	349, 1066, 1665, 3382, 3452, 3846, 3899, 3936, 4081
HgaI	GACGC	3 cut(s)	1829, 2820, 3401
HhaI	GCGC	8 cut(s)	37, 706, 1753, 2415, 3397, 3744, 3981, 4033
Hin6I	GCGC	8 cut(s)	35, 704, 1751, 2413, 3395, 3742, 3979, 4031
HinP1I	GCGC	8 cut(s)	35, 704, 1751, 2413, 3395, 3742, 3979, 4031
HincII	GTYRAC	2 cut(s)	304, 3433
HindII	GTYRAC	2 cut(s)	304, 3433
HindIII	AAGCTT	2 cut(s)	11, 3092
HpaI	GTTAAC	1 cut(s)	304
Hpy166II	GTNNAC	6 cut(s)	304, 1195, 1696, 3372, 3433, 3702
Hpy8I	GTNNAC	6 cut(s)	304, 1195, 1696, 3372, 3433, 3702
Hpy99I	CGWCG	3 cut(s)	313, 331, 383
HspAI	GCGC	8 cut(s)	35, 704, 1751, 2413, 3395, 3742, 3979, 4031
Kpn2I	TCCGGA	1 cut(s)	2851
KpnI	GGTACC	1 cut(s)	3346
Ksp22I	TGATCA	5 cut(s)	904, 1642, 2863, 3019, 3046
KspAI	GTTAAC	1 cut(s)	304
LguI	GCTCTTC	2 cut(s)	2145, 2572
LweI	GCATC	8 cut(s)	382, 691, 1396, 1519, 1729, 2388, 2818, 4083
MabI	ACCWGGT	1 cut(s)	55
MaeI	CTAG	4 cut(s)	756, 1349, 2075, 3572
MbiI	CCGCTC	2 cut(s)	977, 2138
MflI	RGATCY	9 cut(s)	1397, 1486, 1601, 1817, 2476, 2783, 2817, 3250, 3990
MhlI	GDGCHC	2 cut(s)	578, 3268
MlsI	TGGCCA	3 cut(s)	1665, 3452, 3846
MluNI	TGGCCA	3 cut(s)	1665, 3452, 3846
MmeI	TCCRAC	8 cut(s)	247, 291, 350, 1572, 2287, 2717, 3369, 4048
Mox20I	TGGCCA	3 cut(s)	1665, 3452, 3846
MroI	TCCGGA	1 cut(s)	2851
MroXI	GAANNNNTTC	2 cut(s)	1983, 3509
MscI	TGGCCA	3 cut(s)	1665, 3452, 3846
MseI	TTAA	7 cut(s)	303, 2001, 2265, 2331, 2498, 3063, 3144
MslI	CAYNNNNRTG	2 cut(s)	3414, 4040
Msp20I	TGGCCA	3 cut(s)	1665, 3452, 3846
MspA1I	CMGCKG	6 cut(s)	710, 815, 2629, 3275, 3476, 3984
MspR9I	CCNGG	8 cut(s)	57, 1667, 1822, 2280, 2349, 3746, 3848, 3902
Mva1269I	GAATGC	3 cut(s)	1255, 2983, 3664
MvaI	CCWGG	7 cut(s)	57, 1667, 1822, 2280, 3746, 3848, 3902
MvnI	CGCG	1 cut(s)	3395
NciI	CCSGG	1 cut(s)	2349
NdeI	CATATG	1 cut(s)	3796
NlaIV	GGNNCC	9 cut(s)	178, 1305, 1603, 1819, 2596, 2901, 3344, 3357, 3407
NmeAIII	GCCGAG	1 cut(s)	3912
NmuCI	GTSAC	9 cut(s)	164, 764, 798, 968, 1241, 1924, 2254, 3676, 3890
NspI	RCATGY	2 cut(s)	653, 791
NspV	TTCGAA	2 cut(s)	419, 554
PaeI	GCATGC	1 cut(s)	653
PagI	TCATGA	2 cut(s)	1639, 2682
PasI	CCCWGGG	1 cut(s)	3901
PciSI	GCTCTTC	2 cut(s)	2145, 2572
PctI	GAATGC	3 cut(s)	1255, 2983, 3664
PdmI	GAANNNNTTC	2 cut(s)	1983, 3509
Pfl23II	CGTACG	1 cut(s)	3117
PflFI	GACNNNGTC	1 cut(s)	3428
PflMI	CCANNNNNTGG	1 cut(s)	3260
PinAI	ACCGGT	1 cut(s)	3345
Ple19I	CGATCG	1 cut(s)	843
PshBI	ATTAAT	1 cut(s)	2265
Psp6I	CCWGG	7 cut(s)	55, 1665, 1820, 2278, 3744, 3846, 3900
PspFI	CCCAGC	3 cut(s)	395, 1847, 3402
PspGI	CCWGG	7 cut(s)	55, 1665, 1820, 2278, 3744, 3846, 3900
PspLI	CGTACG	1 cut(s)	3117
PspN4I	GGNNCC	9 cut(s)	178, 1305, 1603, 1819, 2596, 2901, 3344, 3357, 3407
PspPI	GGNCC	7 cut(s)	177, 2276, 2522, 2585, 3406, 3898, 4080
PstNI	CAGNNNCTG	5 cut(s)	1136, 1190, 1628, 3275, 4077
PsuI	RGATCY	9 cut(s)	1397, 1486, 1601, 1817, 2476, 2783, 2817, 3250, 3990
PsyI	GACNNNGTC	1 cut(s)	3428
PvuI	CGATCG	1 cut(s)	843
PvuII	CAGCTG	4 cut(s)	710, 815, 3275, 3476
RsaI	GTAC	9 cut(s)	83, 334, 2052, 3119, 3131, 3230, 3344, 3760, 3769
RsaNI	GTAC	9 cut(s)	82, 333, 2051, 3118, 3130, 3229, 3343, 3759, 3768
RseI	CAYNNNNRTG	2 cut(s)	3414, 4040
SapI	GCTCTTC	2 cut(s)	2145, 2572
SaqAI	TTAA	7 cut(s)	303, 2001, 2265, 2331, 2498, 3063, 3144
Sau96I	GGNCC	7 cut(s)	177, 2276, 2522, 2585, 3406, 3898, 4080
ScaI	AGTACT	1 cut(s)	83
ScrFI	CCNGG	8 cut(s)	57, 1667, 1822, 2280, 2349, 3746, 3848, 3902
SduI	GDGCHC	2 cut(s)	578, 3268
SexAI	ACCWGGT	1 cut(s)	55
SfaNI	GCATC	8 cut(s)	382, 691, 1396, 1519, 1729, 2388, 2818, 4083
SfcI	CTRYAG	2 cut(s)	627, 2037
SfuI	TTCGAA	2 cut(s)	419, 554
SinI	GGWCC	5 cut(s)	177, 2276, 2522, 2585, 3406
SmiMI	CAYNNNNRTG	2 cut(s)	3414, 4040
SmlI	CTYRAG	4 cut(s)	1055, 1951, 2008, 3071
SmoI	CTYRAG	4 cut(s)	1055, 1951, 2008, 3071
SpeI	ACTAGT	1 cut(s)	755
SphI	GCATGC	1 cut(s)	653
SspI	AATATT	1 cut(s)	3517
SspMI	CTAG	4 cut(s)	756, 1349, 2075, 3572
StyD4I	CCNGG	8 cut(s)	55, 1665, 1820, 2278, 2347, 3744, 3846, 3900
StyI	CCWWGG	2 cut(s)	357, 2054
TatI	WGTACW	1 cut(s)	81
TauI	GCSGC	5 cut(s)	349, 1283, 3310, 3820, 3939
Tru1I	TTAA	7 cut(s)	303, 2001, 2265, 2331, 2498, 3063, 3144
Tru9I	TTAA	7 cut(s)	303, 2001, 2265, 2331, 2498, 3063, 3144
TscAI	CASTG	5 cut(s)	1705, 1749, 3223, 3390, 3709
TseFI	GTSAC	9 cut(s)	164, 764, 798, 968, 1241, 1924, 2254, 3676, 3890
Tsp45I	GTSAC	9 cut(s)	164, 764, 798, 968, 1241, 1924, 2254, 3676, 3890
TspGWI	ACGGA	6 cut(s)	200, 683, 937, 1139, 1691, 3045
TspRI	CASTG	5 cut(s)	1705, 1749, 3223, 3390, 3709
Tth111I	GACNNNGTC	1 cut(s)	3428
Van91I	CCANNNNNTGG	1 cut(s)	3260
VpaK11BI	GGWCC	5 cut(s)	177, 2276, 2522, 2585, 3406
VspI	ATTAAT	1 cut(s)	2265
XapI	RAATTY	9 cut(s)	140, 314, 487, 550, 1310, 1411, 2108, 2233, 3805
XceI	RCATGY	2 cut(s)	653, 791
XcmI	CCANNNNNNNNNTGG	1 cut(s)	364
XmnI	GAANNNNTTC	2 cut(s)	1983, 3509
XspI	CTAG	4 cut(s)	756, 1349, 2075, 3572
ZrmI	AGTACT	1 cut(s)	83

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.