Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0004317

Physical Location & Seq

Genomic Coordinates Reverse (-)

58728016 .. 58733246

Gene Structure 5231 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG357600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4302 bp

ATGGACTCCTCCAAGCTCCCCGCTCCACAACTCCCTCTGCACTCATCAGGTCTGGGTAGTGACGGTGAGGAGGGGTTCGTCAGTGGGGAGGAGGATTTCGATCCTGGTTTGGATAGGGCGTTTGTCAGAGACGAACAACAAACCCTAGCTCCGGCGGAAGGGGAAGTAGTAGGTAGTAGCAGCAAGGCTGTCAAATTTGTTAGTTCTTCCGAGTTTTTTCTGAGGCCGATTGCCAAGGTCTCCGTGGATGATGATGACGGCGAAGAAGAAGAAGAAGAAGAAGAGGATTCGAAAGCCGGTGTGCAAGTGTTTAAGGAGTCTGTTGGGGTGGGTGAGAGTTCTGAGGTGGAGAAGGGTTTCAATTCCGGTGAGTTGGTGAAAGAGAATGGTGTTGGTGGTGAAGTAGGGAAGTCGAAGGTTGCGGAGGGTTTAGGCTCTGTTTCTGTGGCTGAGATAGAGGATAAGAAGGTTGTAGAGCCTAAGGAGAGCGAGGTTGTTAATGGTACCGCAGAAGCTGTGAGGGAGAGTAGTGAGAATGGCTTGGTTGAAGAACACGGTGTGCAGTTTACTGGTGACTCAGTTGTGCAAGCTGTGCATGTTGATTTGTCACACACAGGAGCAGCTGTTGTTGGCGATGTGGAGAAGATTGAGGAGTCTGAGATAAAAGGGTTGGAGGTTCCGGTAGGGGTGAGCTTGGATAGTGAGTTTGGTCAAATTAGCCGTGAGGTTGAAGAGCCGAAAGATTCAGAATTGGTTGCTGTGGAGTCTAAGTCTGACGGAGTGGTTGTTGCTGAACCAGCAGTTGATAAGTTGGTTGAAGAACATGGTGTTGATATCACTAGTGAAGGTGACTCAGTTGTGGAAGCTGCCCGTGTTGATTTGTCACAGACAGGAGCAGCTGTTGTTGGTGATGTGGAGAAGATTGAGGAATCTGCAATTAAAGGGTTGGAGGTTCCAGCAGGAGTGAGCTTGGAGAATGGAATTGATCAAATTGGCCGTGATTCTGAATTGCCAAATGATTCAAAGTTGGTGGATGTGAACTCTGAGTCTGAAAAAGCGGCTGTTGCTGACCCAGCAGATGATAAGTTGGTTGAAGAACATGGTATGGATTTTACTAGCAAGGGCGATTCAGTTGTGCAAGCTGTCGATGTTGATTTGTCACAGACAGGAGCAGATGTTGTTGGTGATGTGGAGAAGATTGAGGAATCTGAAATTAAAGTTCCAATAGGCACGAGCTTGGAGAATGGATTTGATCAAATTAGCAGTGATGTTGAATTGCAGTCCGAGTCTGACAAAGTGGTTGTTGCTGAGTCGGGGAATGTGGAGCAGAAAACTGTTGTTGCTGCTGCTGAGGAAGTAGATTTCCAGGAGAGAGAGGAGAAAGAACTTGAAAACAATGCTGCAACTGGACAGGAAGGGCCCACAATGGAGATAGGTGCTGCTGAAGTAAAAGTAGTGCCAGATGAAACCCAGTCTGTGAACCATGTTTCTGATTTTAGTGTACTGAATGCACCCGAGAGTGCTGAAAAGAGTGCAACTGACCAAGAAATAAAATTAGAGGAGGAAGAGGAGAAGCACTCTCTTGATGAAAGTGGCATTGAATCTTTAGAACCAAGTTCCATTTCACATCAAGAGATAAGGGTTGAAGCTGAAGATGATGACGAAGATGATGACGATAAGGACGATGAAGGGGAAAATGATGGTATGATATTTGGGAACTCTGCAGCTGCCAAACAATTCTTGGAAGAGTTGGAACGGGAATCAGGTACTGGTTCTTACTCAGGTGCAGAGAGTTACGGTGACCGTTCTTTGAGTTATCGTGATGATTCTCAGAGAGTTGATGGGCAGATTGTGACAGACTCAGATGAAGAAGTGGACACTGATGAAGAAGGGGGTGGAAAAGAGTTATTTGATTCTGCTGCATTGGCAGCTCTTCTGAAAGCAGCAACAGGTGGCGCTGCGTCAGATGGTGGCAGTGTCACATTTACCTCCCCAGATGGATCCAGGCTTTTCTCTGTCGAGCGTCCTGCTGGTTTGGGATCCTCAATGCGGCCTATGAAACCTGGTACACGACCAAACCCTTCTACTCTATTTTCCAATGCTACAGTTGGGGGAGATACTGACAACAGCTTGAGTGAAGAAGAGAAAAAGAAACTGGAAAAATTTCAGCAGATAAGGGTTAAGTTCTTAAGGCTTGTTCAGAGATTAGGTGTATCTACAGAAGATTCAATAGCCAAGCAGGTTCTATACCGCCTAGCTCTTGTTTCAGGAAGGCAAAATAGTCGTGAGTTTAGCCTTGAAGCTGCAAAGACAACAGCTCTTGAGCTTGAAGCAGATGGGAAAGACGATCTTGACTTTTCCTTGAACATACTGGTTCTTGGGAAAACAGGTGTGGGGAAGAGTGCTACTATTAATTCTATTTTTGGTGAAGAAAAGACTCCAATTTATGCCTTTGGACCAGCAACAACCACTGTGAAAGAAATTGTTGGAGTGGTGGATGGAATCAAGATTAGGGTTTTTGATACACCAGGTCTTAAATCTGCTGCAATGGAACAAAATGTAAACCGCAAAATTTTATCTTCTGTGCAGAAGTTCACGAAAAAATGCCCCCCAGATATTGTCCTCTATGTGGATCGGTTGGACACCCAAAGTAGGGATCTAAATGATGTGCCAGTATTAAGATCAATTACTACTGCTTTTGGCCCTTCAATTTGGCGAAGTACTATAGTCACTCTAACCCATGCTGCTTCTGCTCCTCCTGATGGACCGTCAGGCTCTCCTCTGAATTATGAGTTGTTTGTTGCTCAGAGGTCACAGATCCTCCAGCAGACCATTGGACAAGCTATTGGGGATATAAGGTTTACAAATCCAAATATGATGAGTCCTATATCTCTTGTAGAGAACCATCCCTCCTGTCGAAAGAATAGAGAAGGTGAGAAAGTGCTCCCTAATGGTCAAAGCTGGAGACCTCAGCTATTGCTCTTGAGCTCTTCTATGAAGGTCTTAGCTGAGGCAACTAATCTCTCAAAGCCTGCAGAACCATTTGATCACCGAAAACTATTTGGTTTCCGCACCCGTTCAGCTCCTCTTCCATACTTGTTGTCTTGGCTGCTGCAGTCTCGTACCCATCCAAAGCTCTCTGGTGATCAAGGTGGTGAGAATGCGGACTCAGATATCGACTTGGCTGACTTGTCCGATTCTGATAATGAGGAAGAGGAGGATGAGTATGATCAGCTTCCATCATTCAAGCCTCTCAAGAAATCTCAGATTGCCAAGCTTAGCAAAGAGCAGAAGAAGGCGTACCTTGAGGAGTACGATTACAGGGTTAAGCTCCTCCAGAAGAAGATGTGGAGAGAAGAGTTGAAAAGGATGAAAGAAATGAAGAAGGGTAATAAGGCTAGTGCAGACGAGTATGGTTATCTGGGTGAAGAAGATCCGGAAAATGCTGGCCCAGCAGCTGTGCCAGTTGCATTACCTGATATGGTTCTGCCACCTTCTTTTGATAGTGAAAATCCAGCTTACAGGTACCGCTTCTTGGAGCCAACTTCTCAGTTCTTGGCTAGGCCAGTATTGGATGCCCAAGGTTGGGACCATGACTGTGGGTATGATGGTGTCAATCTTGAATACAGTCAAGCCATTGTTAATACTTTTCCAGCTACTGTTGCTGTTCAAGTTACAAAGGACAAGAAGGAGTTTAATCTCCATTTGGATTCTTCAGTTGCTGCTAAGCATGGGGAGAATGGATCAAGTATGGCAGGTTTTGACATTCAAAACATTGGAAAGCAACTTGCTTACATTGTCAGAGGAGAAACCAAATGGAAAAATTTGAAGAAGAACAAGACTACTGCTGGAGTATCCATGACATTTTTAGGTGAAAATGTATCCACTGGAGTCAAAATTGAGGATCAGATTGCTGTTGGCAAGCGCCTGGTATTTGTGGGTAGTGCTGGAACTGTCAGATCTCAGGGTGACTCGGCATATGGAGCCAATGTAGAGGTGCGGTTGAGGGAAGCAGATTTTCCAATTGGCCAGGATCAATCATCATTGGGTCTGTCTCTGGTGAAGTGGAGGGGTGACTTGGCCCTGGGGGCAAATCTTCAGTCCCAGTTTTCCCTTGGACGAGACTACAAGATAGCTGTTCGTGCAGGATTGAACAACAAGCGCAGCGGACAGATCTCGATTAGAACAAGCAGCTCTGAACAACTTCAAATTGCACTTGTGGCTGTCATTCCAATCGTAAGATCCATCTACAATCTTATCTTCCCTGGAGCTTCACTCAGTGAGAATTACTCAATCTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003924 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006605 GO:0006810 GO:0006886 GO:0007154 GO:0007165 GO:0007186 GO:0008104 GO:0008150 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009941 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017038 GO:0017111 GO:0019867 GO:0023052 GO:0031090 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0038023 GO:0042170 GO:0042886 GO:0043021 GO:0043024 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045036 GO:0045037 GO:0045184 GO:0046907 GO:0050789 GO:0050794 GO:0050896 GO:0051087 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055085 GO:0060089 GO:0061927 GO:0065002 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0072594 GO:0072596 GO:0072598 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AIG1 TOC159_MAD

Protein Analysis

1433

Amino Acids

154.69

Weight (kDa)

4.5

Isoelectric Point (pI)

42.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AIG1	PF04548	788 - 935	4.3e-21	AIG1 family
MMR_HSR1	PF01926	790 - 878	2.9e-09	50S ribosome-binding GTPase
TOC159_MAD	PF11886	1154 - 1418	3.8e-128	Translocase of chloroplast 159/132, membrane anchor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004317)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02482 AT4G02510 AT4G02510 AT4G02510 AT4G02510
fragaria_vesca	FvH4_2g25480
malus_domestica	MD03G1285700.v1.1 MD11G1305100.v1.1
prunus_persica	Prupe.8G258800_v2.0.a1 Prupe.8G258800_v2.0.a1
pyrus_communis	pycom03g22640 pycom11g26690
rosa_chinensis	RchiOBHm_Chr6g0293691
rosa_laevigata	RLG00000011932
rosa_multiflora	Rmu_co8307375.1_g000001 Rmu_co8497971.1_g000001 Rmu_sc0005431.1_g000009
rosa_roxburghii	Rroxscaffold_7G00173980
rosa_rugosa	Rorug06G0237500 Rorug06G0237500
rosa_samantha	Rh4AG062400 Rh4AG063400 Rh4AG067300 Rh4BG061800 Rh4CG068100 Rh4DG056700 Rh6AG350100 Rh6BG357600 Rh6CG364400 Rh6DG351300
rosa_wichuraiana	Rw0G022990 Rw6G030480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	2230, 3746
Acc65I	GGTACC	2 cut(s)	503, 3525
AccB1I	GGYRCC	2 cut(s)	503, 3525
AccBSI	CCGCTC	1 cut(s)	23
AccIII	TCCGGA	1 cut(s)	3436
AcoI	YGGCCR	2 cut(s)	994, 4027
AcsI	RAATTY	4 cut(s)	194, 2162, 2571, 3823
AcuI	CTGAAG	4 cut(s)	1464, 1671, 3699, 4082
AdeI	CACNNNGTG	2 cut(s)	559, 4280
AfaI	GTAC	9 cut(s)	505, 1503, 1768, 2068, 2722, 3124, 3302, 3314, 3527
AflII	CTTAAG	1 cut(s)	2188
AhlI	ACTAGT	1 cut(s)	839
AjnI	CCWGG	9 cut(s)	103, 1365, 2003, 2062, 2527, 3927, 4029, 4083, 4264
AjuI	GAANNNNNNNTTGG	2 cut(s)	1606, 1638
AloI	GAACNNNNNNTCC	2 cut(s)	2894, 2926
Alw21I	GWGCWC	2 cut(s)	2946, 2990
Alw26I	GTCTC	6 cut(s)	123, 244, 2959, 3123, 4059, 4116
AlwNI	CAGNNNCTG	5 cut(s)	515, 1769, 3458, 3659, 3722
Ama87I	CYCGRG	1 cut(s)	1514
Aor13HI	TCCGGA	1 cut(s)	3436
AoxI	GGCC	9 cut(s)	224, 994, 1418, 2051, 2701, 3448, 3563, 4027, 4080
ApaI	GGGCCC	1 cut(s)	1422
ApoI	RAATTY	4 cut(s)	194, 2162, 2571, 3823
ArsI	GACNNNNNNTTYG	2 cut(s)	4085, 4117
AseI	ATTAAT	1 cut(s)	2412
Asp700I	GAANNNNTTC	3 cut(s)	356, 2163, 4203
Asp718I	GGTACC	2 cut(s)	503, 3525
AspLEI	GCGC	3 cut(s)	1958, 3927, 4164
AspS9I	GGNCC	8 cut(s)	1418, 1419, 2456, 2702, 2765, 3449, 3589, 4081
AsuII	TTCGAA	1 cut(s)	290
AvaI	CYCGRG	1 cut(s)	1514
AvaII	GGWCC	3 cut(s)	2456, 2765, 3589
AxyI	CCTNAGG	1 cut(s)	480
BaeGI	GKGCMC	1 cut(s)	1422
BalI	TGGCCA	1 cut(s)	4029
BamHI	GGATCC	2 cut(s)	2000, 2039
BanI	GGYRCC	2 cut(s)	503, 3525
BanII	GRGCYC	2 cut(s)	1422, 2990
BarI	GAAGNNNNNNTAC	2 cut(s)	3284, 3316
BauI	CACGAG	1 cut(s)	1231
Bbv12I	GWGCWC	2 cut(s)	2946, 2990
BbvCI	CCTCAGC	3 cut(s)	1350, 2970, 3009
BceAI	ACGGC	3 cut(s)	274, 705, 981
BcgI	CGANNNNNNTGC	4 cut(s)	2009, 2043, 2264, 2298
BciT130I	CCWGG	9 cut(s)	105, 1367, 2005, 2064, 2529, 3929, 4031, 4085, 4266
BciVI	GTATCC	2 cut(s)	3865, 3892
BclI	TGATCA	5 cut(s)	985, 1252, 3046, 3145, 3229
BcoDI	GTCTC	6 cut(s)	123, 244, 2959, 3123, 4059, 4116
BcuI	ACTAGT	1 cut(s)	839
BfaI	CTAG	7 cut(s)	146, 840, 1116, 2255, 3399, 3561, 4300
BfmI	CTRYAG	6 cut(s)	1722, 2103, 2217, 2724, 3033, 3113
BfoI	RGCGCY	2 cut(s)	1959, 3928
BfrI	CTTAAG	1 cut(s)	2188
BfuAI	ACCTGC	2 cut(s)	2230, 3746
BfuI	GTATCC	2 cut(s)	3865, 3892
BglI	GCCNNNNNGGC	1 cut(s)	4088
BglII	AGATCT	2 cut(s)	3959, 4173
BlpI	GCTNAGC	2 cut(s)	3278, 3726
BmcAI	AGTACT	1 cut(s)	2722
Bme1390I	CCNGG	9 cut(s)	105, 1367, 2005, 2064, 2529, 3929, 4031, 4085, 4266
Bme18I	GGWCC	3 cut(s)	2456, 2765, 3589
BmeT110I	CYCGRG	1 cut(s)	1514
BmgT120I	GGNCC	8 cut(s)	1418, 1419, 2456, 2702, 2765, 3449, 3589, 4081
BmrFI	CCNGG	9 cut(s)	105, 1367, 2005, 2064, 2529, 3929, 4031, 4085, 4266
BmrI	ACTGGG	2 cut(s)	1465, 4099
BmsI	GCATC	1 cut(s)	3565
BmuI	ACTGGG	2 cut(s)	1465, 4099
BplI	GAGNNNNNCTC	5 cut(s)	1562, 1594, 4260, 4292, 4274
BpmI	CTGGAG	6 cut(s)	2807, 2983, 3320, 3870, 3909, 4287
Bpu10I	CCTNAGC	3 cut(s)	1350, 2970, 3009
Bpu1102I	GCTNAGC	2 cut(s)	3278, 3726
Bpu14I	TTCGAA	1 cut(s)	290
BpuEI	CTTGAG	5 cut(s)	2152, 2342, 3004, 3239, 3326
BsaBI	GATNNNNATC	1 cut(s)	99
BsaI	GGTCTC	2 cut(s)	244, 2959
BsaJI	CCNNGG	7 cut(s)	234, 243, 3580, 4083, 4084, 4114, 4264
BsaWI	WCCGGW	3 cut(s)	365, 679, 3436
Bse118I	RCCGGY	1 cut(s)	296
Bse21I	CCTNAGG	1 cut(s)	480
Bse3DI	GCAATG	1 cut(s)	2553
Bse8I	GATNNNNATC	1 cut(s)	99
BseAI	TCCGGA	1 cut(s)	3436
BseBI	CCWGG	9 cut(s)	105, 1367, 2005, 2064, 2529, 3929, 4031, 4085, 4266
BseDI	CCNNGG	7 cut(s)	234, 243, 3580, 4083, 4084, 4114, 4264
BseGI	GGATG	8 cut(s)	253, 1039, 2503, 2905, 3127, 3226, 3375, 3580
BseJI	GATNNNNATC	1 cut(s)	99
BseMI	GCAATG	1 cut(s)	2553
BseSI	GKGCMC	1 cut(s)	1422
BseYI	CCCAGC	2 cut(s)	1072, 3451
BsgI	GTGCAG	6 cut(s)	23, 581, 1806, 2606, 3423, 4164
BshFI	GGCC	9 cut(s)	226, 996, 1420, 2053, 2703, 3450, 3565, 4029, 4082
BshNI	GGYRCC	2 cut(s)	503, 3525
BsiHKAI	GWGCWC	2 cut(s)	2946, 2990
BsiHKCI	CYCGRG	1 cut(s)	1514
BsiSI	CCGG	5 cut(s)	152, 297, 366, 680, 3437
BslFI	GGGAC	2 cut(s)	3602, 4087
BsmAI	GTCTC	6 cut(s)	123, 244, 2959, 3123, 4059, 4116
BsmBI	CGTCTC	1 cut(s)	123
BsmFI	GGGAC	2 cut(s)	3602, 4087
BsmI	GAATGC	2 cut(s)	1513, 3166
BsnI	GGCC	9 cut(s)	226, 996, 1420, 2053, 2703, 3450, 3565, 4029, 4082
Bso31I	GGTCTC	2 cut(s)	244, 2959
BsoBI	CYCGRG	1 cut(s)	1514
Bsp119I	TTCGAA	1 cut(s)	290
Bsp120I	GGGCCC	1 cut(s)	1418
Bsp1286I	GDGCHC	3 cut(s)	1422, 2946, 2990
Bsp13I	TCCGGA	1 cut(s)	3436
Bsp1720I	GCTNAGC	2 cut(s)	3278, 3726
BspANI	GGCC	9 cut(s)	226, 996, 1420, 2053, 2703, 3450, 3565, 4029, 4082
BspEI	TCCGGA	1 cut(s)	3436
BspMAI	CTGCAG	3 cut(s)	1726, 3037, 3117
BspMI	ACCTGC	2 cut(s)	2230, 3746
BspQI	GCTCTTC	3 cut(s)	726, 1938, 2995
BspT104I	TTCGAA	1 cut(s)	290
BspT107I	GGYRCC	2 cut(s)	503, 3525
BspTI	CTTAAG	1 cut(s)	2188
BspTNI	GGTCTC	2 cut(s)	244, 2959
BsrBI	CCGCTC	1 cut(s)	23
BsrDI	GCAATG	1 cut(s)	2553
BsrFI	RCCGGY	1 cut(s)	296
BssAI	RCCGGY	1 cut(s)	296
BssECI	CCNNGG	7 cut(s)	234, 243, 3580, 4083, 4084, 4114, 4264
BssSI	CACGAG	1 cut(s)	1231
BssT1I	CCWWGG	3 cut(s)	234, 3580, 4114
Bst2BI	CACGAG	1 cut(s)	1231
Bst2UI	CCWGG	9 cut(s)	105, 1367, 2005, 2064, 2529, 3929, 4031, 4085, 4266
BstAFI	CTTAAG	1 cut(s)	2188
BstAPI	GCANNNNNTGC	1 cut(s)	592
BstBI	TTCGAA	1 cut(s)	290
BstC8I	GCNNGC	5 cut(s)	588, 1140, 3033, 3448, 3923
BstDSI	CCRYGG	1 cut(s)	243
BstEII	GGTNACC	1 cut(s)	1799
BstF5I	GGATG	8 cut(s)	253, 1039, 2503, 2905, 3127, 3226, 3375, 3580
BstH2I	RGCGCY	2 cut(s)	1959, 3928
BstHHI	GCGC	3 cut(s)	1958, 3927, 4164
BstMAI	GTCTC	6 cut(s)	123, 244, 2959, 3123, 4059, 4116
BstNI	CCWGG	9 cut(s)	105, 1367, 2005, 2064, 2529, 3929, 4031, 4085, 4266
BstNSI	RCATGY	1 cut(s)	599
BstPI	GGTNACC	1 cut(s)	1799
BstSCI	CCNGG	9 cut(s)	103, 1365, 2003, 2062, 2527, 3927, 4029, 4083, 4264
BstSFI	CTRYAG	6 cut(s)	1722, 2103, 2217, 2724, 3033, 3113
BstSLI	GKGCMC	1 cut(s)	1422
BstX2I	RGATCY	8 cut(s)	2000, 2039, 2656, 2817, 3433, 3959, 4173, 4241
BstXI	CCANNNNNNTGG	1 cut(s)	3599
BstYI	RGATCY	8 cut(s)	2000, 2039, 2656, 2817, 3433, 3959, 4173, 4241
Bsu36I	CCTNAGG	1 cut(s)	480
BsuI	GTATCC	2 cut(s)	3865, 3892
BsuRI	GGCC	9 cut(s)	226, 996, 1420, 2053, 2703, 3450, 3565, 4029, 4082
BtgI	CCRYGG	1 cut(s)	243
BtgZI	GCGATG	1 cut(s)	648
BtsCI	GGATG	8 cut(s)	253, 1039, 2503, 2905, 3127, 3226, 3375, 3580
BtsI	GCAGTG	2 cut(s)	1270, 1981
BtsIMutI	CAGTG	7 cut(s)	88, 1270, 1878, 1981, 2469, 3885, 4285
BveI	ACCTGC	2 cut(s)	2230, 3746
Cac8I	GCNNGC	5 cut(s)	588, 1140, 3033, 3448, 3923
CaiI	CAGNNNCTG	5 cut(s)	515, 1769, 3458, 3659, 3722
CfoI	GCGC	3 cut(s)	1958, 3927, 4164
Cfr10I	RCCGGY	1 cut(s)	296
Cfr13I	GGNCC	8 cut(s)	1418, 1419, 2456, 2702, 2765, 3449, 3589, 4081
CseI	GACGC	2 cut(s)	1950, 2012
CsiI	ACCWGGT	2 cut(s)	2062, 2527
Csp6I	GTAC	9 cut(s)	504, 1502, 1767, 2067, 2721, 3123, 3301, 3313, 3526
CspCI	CAANNNNNGTGG	2 cut(s)	1011, 1046
CviAII	CATG	8 cut(s)	596, 824, 1100, 1484, 2741, 3593, 3731, 3859
CviQI	GTAC	9 cut(s)	504, 1502, 1767, 2067, 2721, 3123, 3301, 3313, 3526
DraIII	CACNNNGTG	2 cut(s)	559, 4280
EaeI	YGGCCR	2 cut(s)	994, 4027
EciI	GGCGGA	1 cut(s)	170
Ecl136II	GAGCTC	1 cut(s)	2988
Eco130I	CCWWGG	3 cut(s)	234, 3580, 4114
Eco24I	GRGCYC	2 cut(s)	1422, 2990
Eco31I	GGTCTC	2 cut(s)	244, 2959
Eco32I	GATATC	2 cut(s)	835, 3175
Eco47I	GGWCC	3 cut(s)	2456, 2765, 3589
Eco53kI	GAGCTC	1 cut(s)	2988
Eco57I	CTGAAG	4 cut(s)	1464, 1671, 3699, 4082
Eco81I	CCTNAGG	1 cut(s)	480
Eco88I	CYCGRG	1 cut(s)	1514
Eco91I	GGTNACC	1 cut(s)	1799
EcoICRI	GAGCTC	1 cut(s)	2988
EcoO109I	RGGNCCY	1 cut(s)	1418
EcoO65I	GGTNACC	1 cut(s)	1799
EcoRII	CCWGG	9 cut(s)	103, 1365, 2003, 2062, 2527, 3927, 4029, 4083, 4264
EcoRV	GATATC	2 cut(s)	835, 3175
EcoT14I	CCWWGG	3 cut(s)	234, 3580, 4114
EcoT38I	GRGCYC	2 cut(s)	1422, 2990
ErhI	CCWWGG	3 cut(s)	234, 3580, 4114
Esp3I	CGTCTC	1 cut(s)	123
FaeI	CATG	8 cut(s)	599, 827, 1103, 1487, 2744, 3596, 3734, 3862
FalI	AAGNNNNNCTT	2 cut(s)	2334, 2366
FaqI	GGGAC	2 cut(s)	3602, 4087
FatI	CATG	8 cut(s)	595, 823, 1099, 1483, 2740, 3592, 3730, 3858
FauI	CCCGC	1 cut(s)	28
FauNDI	CATATG	1 cut(s)	3979
FbaI	TGATCA	5 cut(s)	985, 1252, 3046, 3145, 3229
FokI	GGATG	8 cut(s)	260, 1046, 2510, 2892, 3114, 3233, 3382, 3587
FriOI	GRGCYC	2 cut(s)	1422, 2990
FspBI	CTAG	7 cut(s)	146, 840, 1116, 2255, 3399, 3561, 4300
GlaI	GCGC	3 cut(s)	1957, 3926, 4163
GsaI	CCCAGC	2 cut(s)	1076, 3455
GsuI	CTGGAG	6 cut(s)	2807, 2983, 3320, 3870, 3909, 4287
HaeII	RGCGCY	2 cut(s)	1959, 3928
HaeIII	GGCC	9 cut(s)	226, 996, 1420, 2053, 2703, 3450, 3565, 4029, 4082
HapII	CCGG	5 cut(s)	152, 297, 366, 680, 3437
HgaI	GACGC	2 cut(s)	1950, 2012
HhaI	GCGC	3 cut(s)	1958, 3927, 4164
Hin1II	CATG	8 cut(s)	599, 827, 1103, 1487, 2744, 3596, 3734, 3862
Hin6I	GCGC	3 cut(s)	1956, 3925, 4162
HinP1I	GCGC	3 cut(s)	1956, 3925, 4162
HindIII	AAGCTT	1 cut(s)	3275
HpaII	CCGG	5 cut(s)	152, 297, 366, 680, 3437
Hpy166II	GTNNAC	9 cut(s)	567, 1039, 1480, 1502, 1876, 2069, 2563, 2595, 2862
Hpy8I	GTNNAC	9 cut(s)	567, 1039, 1480, 1502, 1876, 2069, 2563, 2595, 2862
Hsp92II	CATG	8 cut(s)	599, 827, 1103, 1487, 2744, 3596, 3734, 3862
HspAI	GCGC	3 cut(s)	1956, 3925, 4162
Kpn2I	TCCGGA	1 cut(s)	3436
KpnI	GGTACC	2 cut(s)	507, 3529
Ksp22I	TGATCA	5 cut(s)	985, 1252, 3046, 3145, 3229
LguI	GCTCTTC	3 cut(s)	726, 1938, 2995
LweI	GCATC	1 cut(s)	3565
MabI	ACCWGGT	2 cut(s)	2062, 2527
MaeI	CTAG	7 cut(s)	146, 840, 1116, 2255, 3399, 3561, 4300
MbiI	CCGCTC	1 cut(s)	23
MfeI	CAATTG	1 cut(s)	4023
MflI	RGATCY	8 cut(s)	2000, 2039, 2656, 2817, 3433, 3959, 4173, 4241
MhlI	GDGCHC	3 cut(s)	1422, 2946, 2990
MlsI	TGGCCA	1 cut(s)	4029
MluNI	TGGCCA	1 cut(s)	4029
MmeI	TCCRAC	5 cut(s)	651, 927, 1731, 2467, 2619
Mox20I	TGGCCA	1 cut(s)	4029
MroI	TCCGGA	1 cut(s)	3436
MroXI	GAANNNNTTC	3 cut(s)	356, 2163, 4203
MscI	TGGCCA	1 cut(s)	4029
MslI	CAYNNNNRTG	1 cut(s)	3597
Msp20I	TGGCCA	1 cut(s)	4029
MspA1I	CMGCKG	5 cut(s)	623, 899, 1727, 3458, 4167
MspCI	CTTAAG	1 cut(s)	2188
MspI	CCGG	5 cut(s)	152, 297, 366, 680, 3437
MspR9I	CCNGG	9 cut(s)	105, 1367, 2005, 2064, 2529, 3929, 4031, 4085, 4266
MunI	CAATTG	1 cut(s)	4023
Mva1269I	GAATGC	2 cut(s)	1513, 3166
MvaI	CCWGG	9 cut(s)	105, 1367, 2005, 2064, 2529, 3929, 4031, 4085, 4266
NdeI	CATATG	1 cut(s)	3979
NlaIII	CATG	8 cut(s)	599, 827, 1103, 1487, 2744, 3596, 3734, 3862
NmeAIII	GCCGAG	1 cut(s)	3953
NspI	RCATGY	1 cut(s)	599
NspV	TTCGAA	1 cut(s)	290
PasI	CCCWGGG	1 cut(s)	4084
PciSI	GCTCTTC	3 cut(s)	726, 1938, 2995
PcsI	WCGNNNNNNNCGW	1 cut(s)	1680
PctI	GAATGC	2 cut(s)	1513, 3166
PdmI	GAANNNNTTC	3 cut(s)	356, 2163, 4203
PfoI	TCCNGGA	1 cut(s)	1365
PshBI	ATTAAT	1 cut(s)	2412
Psp124BI	GAGCTC	1 cut(s)	2990
Psp6I	CCWGG	9 cut(s)	103, 1365, 2003, 2062, 2527, 3927, 4029, 4083, 4264
PspEI	GGTNACC	1 cut(s)	1799
PspFI	CCCAGC	2 cut(s)	1072, 3451
PspGI	CCWGG	9 cut(s)	103, 1365, 2003, 2062, 2527, 3927, 4029, 4083, 4264
PspOMI	GGGCCC	1 cut(s)	1418
PspPI	GGNCC	8 cut(s)	1418, 1419, 2456, 2702, 2765, 3449, 3589, 4081
PstI	CTGCAG	3 cut(s)	1726, 3037, 3117
PstNI	CAGNNNCTG	5 cut(s)	515, 1769, 3458, 3659, 3722
PsuI	RGATCY	8 cut(s)	2000, 2039, 2656, 2817, 3433, 3959, 4173, 4241
PvuII	CAGCTG	4 cut(s)	623, 899, 1727, 3458
RsaI	GTAC	9 cut(s)	505, 1503, 1768, 2068, 2722, 3124, 3302, 3314, 3527
RsaNI	GTAC	9 cut(s)	504, 1502, 1767, 2067, 2721, 3123, 3301, 3313, 3526
RseI	CAYNNNNRTG	1 cut(s)	3597
SacI	GAGCTC	1 cut(s)	2990
SapI	GCTCTTC	3 cut(s)	726, 1938, 2995
Sau96I	GGNCC	8 cut(s)	1418, 1419, 2456, 2702, 2765, 3449, 3589, 4081
ScaI	AGTACT	1 cut(s)	2722
ScrFI	CCNGG	9 cut(s)	105, 1367, 2005, 2064, 2529, 3929, 4031, 4085, 4266
SduI	GDGCHC	3 cut(s)	1422, 2946, 2990
SexAI	ACCWGGT	2 cut(s)	2062, 2527
SfaNI	GCATC	1 cut(s)	3565
SfcI	CTRYAG	6 cut(s)	1722, 2103, 2217, 2724, 3033, 3113
SfuI	TTCGAA	1 cut(s)	290
SinI	GGWCC	3 cut(s)	2456, 2765, 3589
SmiMI	CAYNNNNRTG	1 cut(s)	3597
SmlI	CTYRAG	6 cut(s)	2131, 2188, 2321, 2983, 3254, 3305
SmoI	CTYRAG	6 cut(s)	2131, 2188, 2321, 2983, 3254, 3305
SpeI	ACTAGT	1 cut(s)	839
SspMI	CTAG	7 cut(s)	146, 840, 1116, 2255, 3399, 3561, 4300
SstI	GAGCTC	1 cut(s)	2990
StyD4I	CCNGG	9 cut(s)	103, 1365, 2003, 2062, 2527, 3927, 4029, 4083, 4264
StyI	CCWWGG	3 cut(s)	234, 3580, 4114
TaqI	TCGA	8 cut(s)	99, 290, 413, 1146, 2019, 2917, 3177, 4178
TatI	WGTACW	2 cut(s)	1501, 2720
TauI	GCSGC	2 cut(s)	1061, 2053
TscAI	CASTG	7 cut(s)	88, 1270, 1885, 1981, 2476, 3892, 4285
TspDTI	ATGAA	9 cut(s)	1479, 1602, 1701, 1881, 1899, 2072, 3011, 3386, 3395
TspGWI	ACGGA	2 cut(s)	232, 792
TspRI	CASTG	7 cut(s)	88, 1270, 1885, 1981, 2476, 3892, 4285
Vha464I	CTTAAG	1 cut(s)	2188
VpaK11BI	GGWCC	3 cut(s)	2456, 2765, 3589
VspI	ATTAAT	1 cut(s)	2412
XapI	RAATTY	4 cut(s)	194, 2162, 2571, 3823
XceI	RCATGY	1 cut(s)	599
XcmI	CCANNNNNNNNNTGG	4 cut(s)	241, 1738, 2831, 4112
XmnI	GAANNNNTTC	3 cut(s)	356, 2163, 4203
XspI	CTAG	7 cut(s)	146, 840, 1116, 2255, 3399, 3561, 4300
ZrmI	AGTACT	1 cut(s)	2722

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.