Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0004317

Physical Location & Seq

Genomic Coordinates Reverse (-)

41981315 .. 41985739

Gene Structure 4425 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1305100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4425 bp

ATGGAGTCCAAGCTTTCTGCTTCCGAGGAGCAGGCTCAGCACCTCTCAAACGCACCAGGTTCTTCCTTTTTTTCTTCGCAGTACTCTGATATCGATGATTCATCGAAAATCCATGTTTCCGAAATTGATAACGACGAGAAATTTAGCGGGGTTGGTGGCCGTGGTGACGGTGGCGGGTCGGAGACTGAGGAGGGGTTTGTGAGTGGGGAGGAGGATTTTGTTTCCGAGAGGGCGTTTGTGAGAGGCGCTGGAGGCGGAGAAACCGTGGTGGTTGGGGGTGGTGCGGGAGAGGAGGGGGATTCCGGTGTTAAGTTTGTTAACTCGTCGGAATTCTTCTATCCGACGAGTAGGCTGCGGCCGGTTGCCAAGGTTTCGGTGGATGACGACGAGGAGGAGGAGGATACCAGGGAAGAAGGGGTTAGGGATTTGAAGAGTTTAGAGGGTGGGGGTTCTAACGGTGTCGTTTGGGGGGATAGTGGGATGGGGAAGAGTGAAATTTTGGGTGAAGTGGTCACGGAAAATGGTGTTAGTGGTGGTGAAGTTGATAACGTAGTTGAGAATTCGAAATTGGTTGAGGGTTTAGGCTCGGTGGTCGAGGAAACCCCGGCAGTGGAGAAGCCGGTGGAGGTGGCTGAAGTAGATGATAAGGAGGCTGTAGATAAGTTTGTTGAAGTTGAAGAGAATGTTATGGAAATTACTAGTGGAGGTGACTCAATTGTGCAGGATATACATGCCAATTTGTCACAGACAGGAGCAGCTGTTGTCGTTGGTGATGTAGAGACGATTGAGGAATCTGAAATAAAAGGCTTGATGGTTGATAAAGGTGTGAGTTTGGATAATGGTTTTGATCAAATTAGCCGGGAGGTGGCTGAAGTAGATGATAAGGAGGTTGTAGAGCAGCATAAGGATGAGGATGTTTTCAATGATGAGACAGAAGTTGTGAGAGAGAGTAAAGAAAATGGCGTGGTGGGGGATCGGCCAGCTGATGATAAGTTTGTTGAAGTTGAAGAGAACGGTGTGGAAATTACAACCGGAGGTGACTCAATTGTGCAGGATATACATGCCAATTTGTCACAGACAGGAGCAGCTATTGTCGTTGGTGATGTAGAGATGATTGAGGAATCTGAAATAAAAGGCTTAGTGGTTGAAGAAGGTGTGAGTTTGGATAATGTTTTTGATCAAATTAGCCGTGAGACCAAAGAACCAAATGACTCGGAGTCAGTGGCTGTGGAATATTTGCCTGACAGAGCGGTTGTTCCTGAGTCTGGGAATGTGGAACTTGATGGAGAGAAAGCTGTTGTTGCTGCTAGTGCGGATGAAGTTGATCTCGAGAAGAGGCCAGAAAGGGAGGATGGACTGAAATCTGATTCCGAAACCAGAGAGAAAGGGATGGCGAGGGAATTAGGTGCTGATGTGGTTCCAGATGACAATCTATTGGTTGGGGATGGAGCTGAAATGGAAACTGTGAACGTTGTTTCTGGTTTAGCAGAACAAGAACCAGAGACTGAGGCAAAACTGGGTAACAGTGACTTGAGTGTACATGATCAAGTGCATGGATTGAGAGCGGCTGTCCCTGGAAAATATGTGGCACCTGAGTTTCTGGATTCAAGTTCTAATAGCCGAGAAGTAAAGCTAGATGAGGAAGTTGAGGAGAAGCATTCCCCTGATGAAGTTGGCATTGAATCTGTGGGTCCAAATTCCATTTCAAACCGAGAGATAAGAGTCGAAACTGATGATGGTGATGGCAATAAGGATCTTCAAGATGATGATGATAATGATCTTCAAGATGATGATCAGGATCTTCAAGATGATGAAGGTGAGAATGAAGGTTCAGTTACAGATGCGAACAATGAAGGTATGATTTTTGGAAGCTCTGAAGCTGCTAAACAGTTTTTGGAAGAGTTGGAGCGAGGATCAGGTGCTGGCTCTTACTCAGGTGCTGAGAGTTATCATGATCATTCACAGAGAATTGATGGCCAGATTGTTACGGATTCGGATGAAGAAGTGGACACTGATGAGGAAGGGGGCGGAAAAGAGTTATTTGATTCAGCTGCGTTGGCAGCTCTTTTGAAAGCAGCAACAGGTGCTTCCTCAGATGGTGGCAATGTTACATTTACCACCCCAGATGGATCCAGGCTTTTCTCTGTTGAGCGTCCTGCTGGGTTGGGGTCCTCAATTCGGTCGTTGAAACCTGCTTCACGACCAAACAACTCGAACCTTATGACATCTTCCAATGCCACAGTTGGGGGAGGTTCTGAGAACTTGAGCGAAGAAGAGAGAGCGAAGCTCGAAAAGTTTCAGCAGATAAGGGTTAAGTTCTTGAGGCTTGTTCAGAGATTAGGTGTTTCTACAGAAGAGTCAGTGCCAAGACAGGTTCTATACCGCCTAGCTCTCGTGTCAGGGAGGCAAAACAGTCAAGAATTTAGCCTCGAAGCTGCAAAGATGACCGCTCTCCAGCTTGAAGCAGAGGAGAAAGATGATTTGGACTTCTCCTTGAACATATTGGTTCTTGGGAAAGCAGGTGTTGGGAAGAGTGCCACTATAAATTCTATTTTCGGTGCAGAAGTTACCCCAATTAATGCTTTTGGACCTGGAACAACTACTGTGAAAAAAATTGTTGGAGTGGTGGATGGAGTCAAGATTAAGGTGTTTGATACACCAGGTCTGAAATCTGCTGCAATGGAACAGGGCATAAATCGCAAAATTTTATCTTCTGTGCAGAGGCATATGAAAAAATCCCCCCCAGATATTGTCCTCTATGTGGATCGGATGGACTCCCGAGGTAGGGATCTAAGTGATGTCCTATTGTTAAGATCAATCACTAATGCCTTTGGTCCTTCAATTTGGCGAAGCACCATAGTTACTCTGACTCACAGCTCTTCTGCTCCTCCTGATGGACCATCAGGCTCCCCGCTGAATTATGAGATGTTTCGTAGTCAGCGGGAACAAATCATGCAGCAGACGATTGGACAAGCTGTTGGTGACCTAAGGTTTATGAATCCTAGTATGATGAGTCCAATATCTCTTGTAGAGAACCACCCGTCTTGTCGGAAGAGAGATAATCAGAAAGTGCTCCCTAATGGTGTAGCTTGGAGATCCCATCTATTGCTCTTGTGCTACTCTATGAAGATCTTGTCTGATGCGTCTAATCTCTCAAAACCTCAGGAATCATTTGATCACCGTAAGCTCTTTGGTTTGCGTTCCCGTTCGGCTCCTCTTCCATACTTGTTGTCTTGGCTGTTGCAATCTCGTCCGCACCCAAAACTTGCTACCGATCAAGGTGGTGAGAATGCTGATTCAGACATTGACTTGGCTGACTTGTCTGATTCTGATCAAGAGGAAGAAGAGGATGAGTATGATCAGCTTCCGTCATTTAAGCCCCTCAAGAAGGCCCAGATTGCTAAGCTTAGTAGAGAGCAGAGGAAAGCGTACGCTGAGGAGTATGATTATAGGGTTAAGCTCCTCCAGAAAAAGATGTGGAAAGAAGAGTTGAGAAGGATGAAGGAAATGAAGAAGAAGGGCAAGGTAAGTGAAGAGGAGTATGGTTATTTGGGTGAAGAGGATCCAGAAAACGGTGCTCCAGCAGCTGTGCCGGTTGCATTACCTGATATGGTTTTGCCGCCTTCTTTTGATGGTGAAAATCCAGCTCACAGGTACCGGTTCCTGGAGCCGACTTCTCAGTTCACGGCAAGGCCAGTGTTGGACCCCCAAGGCTGGGACCATGACTGTGGGTACGATGGTGTCAACCTTGAACATAGTCTGGCCATTGCTAATTCTTTTCCAGCAGCTGTTGCTGTTCAGCTTACAAAGGATAAGAAAGAGTTCAATCTCCATTTAGATTCCTCGGTTGGTGCTAAGCACGGGGAGAACTGGTCGAGTATGCTAGGGTTTGACATTCAGAACATTGGAAAGCAACTTGCATACATTGTGCGAGGAGACACCAAAGTCAAGACCTCCAAGAGGTTCAAGAATGCTGCTGGAGTATCTGTGACATTTCTGGGAGAAAATGTGTCCACTGGACTCAAAGTTGAACAACAGTATGCGGTTGGCAAGCGTCTGGTATTGGTGAGTAGTACTGGTACTGTCCGATCTCAGGGCGAGTCAGCATATGGAGCAAACTTGGAGGTGCGGCTGAGGGAGGCAGATTTTCCAATTGGCCAGGATCAATCCTCATTGGGTCTGTCTCTAGTGCGGTGGAGGGGTGACTTGGCCCTGGGGGCAAATTTCCAGTCCCAGTTTTCCCTCGGGCGAAACTACAAGATGGCTGTTCGTGCAGGGTTGAACAACAAGCGCAGTGGACAGATCTCAATCAGAACCAGCAGCTCCGAACAACTACAACTTGCTCTTATTGCTGTGATTCCAGTCGTCAAGGCTATCTACAACAGCATCCAGCCCGGAGCCAGTGAGAACTACTCCATCTACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003924 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006605 GO:0006810 GO:0006886 GO:0007154 GO:0007165 GO:0007186 GO:0008104 GO:0008150 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009941 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017038 GO:0017111 GO:0019867 GO:0023052 GO:0031090 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0038023 GO:0042170 GO:0042886 GO:0043021 GO:0043024 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045036 GO:0045037 GO:0045184 GO:0046907 GO:0050789 GO:0050794 GO:0050896 GO:0051087 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055085 GO:0060089 GO:0061927 GO:0065002 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0072594 GO:0072596 GO:0072598 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AIG1 TOC159_MAD

Protein Analysis

1475

Amino Acids

159.76

Weight (kDa)

4.53

Isoelectric Point (pI)

44.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AIG1	PF04548	832 - 966	1.6e-19	AIG1 family
MMR_HSR1	PF01926	834 - 921	5e-09	50S ribosome-binding GTPase
TOC159_MAD	PF11886	1197 - 1461	8.9e-122	Translocase of chloroplast 159/132, membrane anchor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004317)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02482 AT4G02510 AT4G02510 AT4G02510 AT4G02510
fragaria_vesca	FvH4_2g25480
malus_domestica	MD03G1285700.v1.1 MD11G1305100.v1.1
prunus_persica	Prupe.8G258800_v2.0.a1 Prupe.8G258800_v2.0.a1
pyrus_communis	pycom03g22640 pycom11g26690
rosa_chinensis	RchiOBHm_Chr6g0293691
rosa_laevigata	RLG00000011932
rosa_multiflora	Rmu_co8307375.1_g000001 Rmu_co8497971.1_g000001 Rmu_sc0005431.1_g000009
rosa_roxburghii	Rroxscaffold_7G00173980
rosa_rugosa	Rorug06G0237500 Rorug06G0237500
rosa_samantha	Rh4AG062400 Rh4AG063400 Rh4AG067300 Rh4BG061800 Rh4CG068100 Rh4DG056700 Rh6AG350100 Rh6BG357600 Rh6CG364400 Rh6DG351300
rosa_wichuraiana	Rw0G022990 Rw6G030480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2510
AasI	GACNNNNNNGTC	1 cut(s)	3944
Acc36I	ACCTGC	2 cut(s)	2200, 2510
Acc65I	GGTACC	1 cut(s)	3654
AccB1I	GGYRCC	2 cut(s)	1588, 3654
AccBSI	CCGCTC	3 cut(s)	1250, 1565, 2450
AclI	AACGTT	1 cut(s)	1470
AcoI	YGGCCR	6 cut(s)	157, 356, 977, 1975, 3762, 4156
AcsI	RAATTY	9 cut(s)	140, 329, 495, 559, 1696, 2420, 2545, 2703, 4222
AcuI	CTGAAG	3 cut(s)	654, 891, 1896
AfaI	GTAC	7 cut(s)	83, 1539, 3431, 3656, 3734, 4075, 4081
AgeI	ACCGGT	1 cut(s)	3657
AhdI	GACNNNNNGTC	1 cut(s)	1216
AhlI	ACTAGT	1 cut(s)	698
AjnI	CCWGG	9 cut(s)	55, 404, 1573, 2132, 2590, 2659, 3663, 4158, 4212
AjuI	GAANNNNNNNTTGG	6 cut(s)	551, 583, 1687, 1719, 2465, 2497
Alw21I	GWGCWC	2 cut(s)	3075, 3580
Alw26I	GTCTC	7 cut(s)	176, 773, 923, 1187, 1496, 3930, 4188
AlwNI	CAGNNNCTG	6 cut(s)	1226, 1505, 1922, 1940, 3587, 3788
Ama87I	CYCGRG	3 cut(s)	1328, 2778, 4244
ApoI	RAATTY	9 cut(s)	140, 329, 495, 559, 1696, 2420, 2545, 2703, 4222
ArsI	GACNNNNNNTTYG	2 cut(s)	4214, 4246
AseI	ATTAAT	1 cut(s)	2577
AsiGI	ACCGGT	1 cut(s)	3657
Asp700I	GAANNNNTTC	2 cut(s)	2295, 3821
Asp718I	GGTACC	1 cut(s)	3654
AspLEI	GCGC	2 cut(s)	248, 4293
AspS9I	GGNCC	9 cut(s)	1691, 2169, 2588, 2834, 2897, 3391, 3703, 3718, 4210
AsuC2I	CCSGG	3 cut(s)	605, 860, 4395
AsuII	TTCGAA	1 cut(s)	563
AvaI	CYCGRG	3 cut(s)	1328, 2778, 4244
AvaII	GGWCC	7 cut(s)	1691, 2169, 2588, 2834, 2897, 3703, 3718
AxyI	CCTNAGG	2 cut(s)	2987, 3162
BaeI	ACNNNNGTAYC	2 cut(s)	3724, 3757
BalI	TGGCCA	3 cut(s)	1977, 3764, 4158
BamHI	GGATCC	2 cut(s)	2129, 3562
BanI	GGYRCC	2 cut(s)	1588, 3654
BauI	CACGAG	1 cut(s)	2393
Bbv12I	GWGCWC	2 cut(s)	3075, 3580
BbvCI	CCTCAGC	2 cut(s)	3435, 4133
BceAI	ACGGC	3 cut(s)	144, 1173, 3702
BcgI	CGANNNNNNTGC	2 cut(s)	334, 368
BciT130I	CCWGG	9 cut(s)	57, 406, 1575, 2134, 2592, 2661, 3665, 4160, 4214
BciVI	GTATCC	1 cut(s)	394
BclI	TGATCA	8 cut(s)	847, 1177, 1543, 1792, 1954, 3175, 3331, 3358
BcnI	CCSGG	3 cut(s)	605, 860, 4395
BcoDI	GTCTC	7 cut(s)	176, 773, 923, 1187, 1496, 3930, 4188
BcuI	ACTAGT	1 cut(s)	698
BfaI	CTAG	7 cut(s)	699, 1308, 1634, 2387, 3003, 3884, 4187
BfmI	CTRYAG	2 cut(s)	654, 2349
BfoI	RGCGCY	1 cut(s)	249
BfuAI	ACCTGC	2 cut(s)	2200, 2510
BfuI	GTATCC	1 cut(s)	394
BglI	GCCNNNNNGGC	1 cut(s)	4217
BglII	AGATCT	2 cut(s)	3129, 4302
BlpI	GCTNAGC	3 cut(s)	36, 3402, 3855
BmcAI	AGTACT	2 cut(s)	83, 4075
Bme18I	GGWCC	7 cut(s)	1691, 2169, 2588, 2834, 2897, 3703, 3718
BmeRI	GACNNNNNGTC	1 cut(s)	1216
BmeT110I	CYCGRG	3 cut(s)	1328, 2778, 4244
BmgT120I	GGNCC	9 cut(s)	1691, 2169, 2588, 2834, 2897, 3391, 3703, 3718, 4210
BmrI	ACTGGG	2 cut(s)	1526, 4228
BmsI	GCATC	3 cut(s)	1831, 3130, 4395
BmuI	ACTGGG	2 cut(s)	1526, 4228
BplI	GAGNNNNNCTC	3 cut(s)	2271, 2303, 4397
BpmI	CTGGAG	6 cut(s)	270, 2438, 3449, 3564, 3686, 3999
Bpu10I	CCTNAGC	2 cut(s)	3435, 4133
Bpu1102I	GCTNAGC	3 cut(s)	36, 3402, 3855
Bpu14I	TTCGAA	1 cut(s)	563
BpuEI	CTTGAG	4 cut(s)	1552, 2284, 2341, 3368
BpuMI	CCSGG	3 cut(s)	605, 860, 4395
Bsa29I	ATCGAT	1 cut(s)	93
BsaBI	GATNNNNATC	6 cut(s)	1428, 1776, 1791, 1797, 3330, 4307
BsaI	GGTCTC	1 cut(s)	1187
BsaWI	WCCGGW	3 cut(s)	302, 1031, 3657
Bse118I	RCCGGY	4 cut(s)	358, 619, 3592, 3657
Bse1I	ACTGG	9 cut(s)	1521, 3695, 3875, 4021, 4081, 4228, 4234, 4361, 4401
Bse21I	CCTNAGG	2 cut(s)	2987, 3162
Bse3DI	GCAATG	3 cut(s)	2110, 2685, 3765
Bse8I	GATNNNNATC	6 cut(s)	1428, 1776, 1791, 1797, 3330, 4307
BseBI	CCWGG	9 cut(s)	57, 406, 1575, 2134, 2592, 2661, 3665, 4160, 4214
BseCI	ATCGAT	1 cut(s)	93
BseJI	GATNNNNATC	6 cut(s)	1428, 1776, 1791, 1797, 3330, 4307
BseMI	GCAATG	3 cut(s)	2110, 2685, 3765
BseNI	ACTGG	9 cut(s)	1521, 3695, 3875, 4021, 4081, 4228, 4234, 4361, 4401
BseX3I	CGGCCG	1 cut(s)	356
BseYI	CCCAGC	2 cut(s)	2159, 3714
BsgI	GTGCAG	5 cut(s)	740, 1070, 2580, 2738, 4293
Bsh1285I	CGRYCG	2 cut(s)	359, 2183
BshNI	GGYRCC	2 cut(s)	1588, 3654
BshTI	ACCGGT	1 cut(s)	3657
BshVI	ATCGAT	1 cut(s)	93
BsiEI	CGRYCG	2 cut(s)	359, 2183
BsiHKAI	GWGCWC	2 cut(s)	3075, 3580
BsiHKCI	CYCGRG	3 cut(s)	1328, 2778, 4244
BsiSI	CCGG	9 cut(s)	303, 359, 605, 620, 859, 1032, 3593, 3658, 4395
BsiWI	CGTACG	1 cut(s)	3429
BslFI	GGGAC	3 cut(s)	1556, 3731, 4216
BsmAI	GTCTC	7 cut(s)	176, 773, 923, 1187, 1496, 3930, 4188
BsmBI	CGTCTC	1 cut(s)	773
BsmFI	GGGAC	3 cut(s)	1556, 3731, 4216
BsmI	GAATGC	3 cut(s)	1657, 3295, 3976
Bso31I	GGTCTC	1 cut(s)	1187
BsoBI	CYCGRG	3 cut(s)	1328, 2778, 4244
Bsp119I	TTCGAA	1 cut(s)	563
Bsp1286I	GDGCHC	2 cut(s)	3075, 3580
Bsp1407I	TGTACA	1 cut(s)	1537
Bsp1720I	GCTNAGC	3 cut(s)	36, 3402, 3855
BspDI	ATCGAT	1 cut(s)	93
BspHI	TCATGA	1 cut(s)	1951
BspMI	ACCTGC	2 cut(s)	2200, 2510
BspQI	GCTCTTC	1 cut(s)	2884
BspT104I	TTCGAA	1 cut(s)	563
BspT107I	GGYRCC	2 cut(s)	1588, 3654
BspTNI	GGTCTC	1 cut(s)	1187
BsrBI	CCGCTC	3 cut(s)	1250, 1565, 2450
BsrDI	GCAATG	3 cut(s)	2110, 2685, 3765
BsrFI	RCCGGY	4 cut(s)	358, 619, 3592, 3657
BsrGI	TGTACA	1 cut(s)	1537
BsrI	ACTGG	9 cut(s)	1521, 3695, 3875, 4021, 4081, 4228, 4234, 4361, 4401
BssAI	RCCGGY	4 cut(s)	358, 619, 3592, 3657
BssSI	CACGAG	1 cut(s)	2393
BssT1I	CCWWGG	2 cut(s)	366, 3709
Bst2BI	CACGAG	1 cut(s)	2393
Bst2UI	CCWGG	9 cut(s)	57, 406, 1575, 2134, 2592, 2661, 3665, 4160, 4214
BstAPI	GCANNNNNTGC	2 cut(s)	2084, 3791
BstAUI	TGTACA	1 cut(s)	1537
BstBI	TTCGAA	1 cut(s)	563
BstC8I	GCNNGC	4 cut(s)	33, 981, 1924, 4052
BstDSI	CCRYGG	2 cut(s)	160, 264
BstEII	GGTNACC	1 cut(s)	2981
BstENI	CCTNNNNNAGG	2 cut(s)	3386, 3958
BstH2I	RGCGCY	1 cut(s)	249
BstHHI	GCGC	2 cut(s)	248, 4293
BstMAI	GTCTC	7 cut(s)	176, 773, 923, 1187, 1496, 3930, 4188
BstMCI	CGRYCG	2 cut(s)	359, 2183
BstNI	CCWGG	9 cut(s)	57, 406, 1575, 2134, 2592, 2661, 3665, 4160, 4214
BstNSI	RCATGY	2 cut(s)	734, 1064
BstPI	GGTNACC	1 cut(s)	2981
BstSFI	CTRYAG	2 cut(s)	654, 2349
BstX2I	RGATCY	8 cut(s)	1753, 1798, 2129, 2788, 3095, 3129, 3562, 4302
BstXI	CCANNNNNNTGG	1 cut(s)	3728
BstYI	RGATCY	8 cut(s)	1753, 1798, 2129, 2788, 3095, 3129, 3562, 4302
BstZI	CGGCCG	1 cut(s)	356
Bsu15I	ATCGAT	1 cut(s)	93
Bsu36I	CCTNAGG	2 cut(s)	2987, 3162
BsuI	GTATCC	1 cut(s)	394
BsuTUI	ATCGAT	1 cut(s)	93
BtgI	CCRYGG	2 cut(s)	160, 264
BtsI	GCAGTG	2 cut(s)	615, 4300
BtsIMutI	CAGTG	9 cut(s)	615, 1227, 1531, 2010, 2367, 3702, 4014, 4300, 4408
BveI	ACCTGC	2 cut(s)	2200, 2510
Cac8I	GCNNGC	4 cut(s)	33, 981, 1924, 4052
CaiI	CAGNNNCTG	6 cut(s)	1226, 1505, 1922, 1940, 3587, 3788
CciI	TCATGA	1 cut(s)	1951
CfoI	GCGC	2 cut(s)	248, 4293
Cfr10I	RCCGGY	4 cut(s)	358, 619, 3592, 3657
Cfr13I	GGNCC	9 cut(s)	1691, 2169, 2588, 2834, 2897, 3391, 3703, 3718, 4210
ClaI	ATCGAT	1 cut(s)	93
CseI	GACGC	3 cut(s)	2141, 3132, 4043
CsiI	ACCWGGT	2 cut(s)	55, 2659
Csp6I	GTAC	7 cut(s)	82, 1538, 3430, 3655, 3733, 4074, 4080
CspAI	ACCGGT	1 cut(s)	3657
CspCI	CAANNNNNGTGG	4 cut(s)	3026, 3061, 4276, 4311
CviAII	CATG	8 cut(s)	113, 731, 1061, 1541, 1553, 1952, 2953, 3722
CviQI	GTAC	7 cut(s)	82, 1538, 3430, 3655, 3733, 4074, 4080
DrdI	GACNNNNNNGTC	1 cut(s)	3944
DriI	GACNNNNNGTC	1 cut(s)	1216
DseDI	GACNNNNNNGTC	1 cut(s)	3944
EaeI	YGGCCR	6 cut(s)	157, 356, 977, 1975, 3762, 4156
EagI	CGGCCG	1 cut(s)	356
Eam1105I	GACNNNNNGTC	1 cut(s)	1216
EciI	GGCGGA	2 cut(s)	270, 2043
EclXI	CGGCCG	1 cut(s)	356
Eco130I	CCWWGG	2 cut(s)	366, 3709
Eco31I	GGTCTC	1 cut(s)	1187
Eco32I	GATATC	1 cut(s)	91
Eco47I	GGWCC	7 cut(s)	1691, 2169, 2588, 2834, 2897, 3703, 3718
Eco52I	CGGCCG	1 cut(s)	356
Eco57I	CTGAAG	3 cut(s)	654, 891, 1896
Eco81I	CCTNAGG	2 cut(s)	2987, 3162
Eco88I	CYCGRG	3 cut(s)	1328, 2778, 4244
Eco91I	GGTNACC	1 cut(s)	2981
EcoNI	CCTNNNNNAGG	2 cut(s)	3386, 3958
EcoO109I	RGGNCCY	1 cut(s)	2169
EcoO65I	GGTNACC	1 cut(s)	2981
EcoRI	GAATTC	2 cut(s)	329, 559
EcoRII	CCWGG	9 cut(s)	55, 404, 1573, 2132, 2590, 2659, 3663, 4158, 4212
EcoRV	GATATC	1 cut(s)	91
EcoT14I	CCWWGG	2 cut(s)	366, 3709
ErhI	CCWWGG	2 cut(s)	366, 3709
Esp3I	CGTCTC	1 cut(s)	773
FaeI	CATG	8 cut(s)	116, 734, 1064, 1544, 1556, 1955, 2956, 3725
FaqI	GGGAC	3 cut(s)	1556, 3731, 4216
FatI	CATG	8 cut(s)	112, 730, 1060, 1540, 1552, 1951, 2952, 3721
FauI	CCCGC	5 cut(s)	140, 167, 277, 2919, 2934
FauNDI	CATATG	2 cut(s)	2727, 4108
FbaI	TGATCA	8 cut(s)	847, 1177, 1543, 1792, 1954, 3175, 3331, 3358
FspBI	CTAG	7 cut(s)	699, 1308, 1634, 2387, 3003, 3884, 4187
GlaI	GCGC	2 cut(s)	247, 4292
GsaI	CCCAGC	2 cut(s)	2163, 3718
GsuI	CTGGAG	6 cut(s)	270, 2438, 3449, 3564, 3686, 3999
HaeII	RGCGCY	1 cut(s)	249
HapII	CCGG	9 cut(s)	303, 359, 605, 620, 859, 1032, 3593, 3658, 4395
HgaI	GACGC	3 cut(s)	2141, 3132, 4043
HhaI	GCGC	2 cut(s)	248, 4293
Hin1II	CATG	8 cut(s)	116, 734, 1064, 1544, 1556, 1955, 2956, 3725
Hin6I	GCGC	2 cut(s)	246, 4291
HinP1I	GCGC	2 cut(s)	246, 4291
HincII	GTYRAC	2 cut(s)	319, 3745
HindII	GTYRAC	2 cut(s)	319, 3745
HindIII	AAGCTT	2 cut(s)	11, 3404
HpaI	GTTAAC	1 cut(s)	319
HpaII	CCGG	9 cut(s)	303, 359, 605, 620, 859, 1032, 3593, 3658, 4395
Hpy166II	GTNNAC	8 cut(s)	319, 1468, 1538, 2008, 3684, 3745, 4014, 4298
Hpy8I	GTNNAC	8 cut(s)	319, 1468, 1538, 2008, 3684, 3745, 4014, 4298
Hpy99I	CGWCG	4 cut(s)	137, 328, 346, 389
HpyCH4IV	ACGT	2 cut(s)	549, 1470
HpySE526I	ACGT	2 cut(s)	549, 1470
Hsp92II	CATG	8 cut(s)	116, 734, 1064, 1544, 1556, 1955, 2956, 3725
HspAI	GCGC	2 cut(s)	246, 4291
KpnI	GGTACC	1 cut(s)	3658
Ksp22I	TGATCA	8 cut(s)	847, 1177, 1543, 1792, 1954, 3175, 3331, 3358
KspAI	GTTAAC	1 cut(s)	319
LguI	GCTCTTC	1 cut(s)	2884
LweI	GCATC	3 cut(s)	1831, 3130, 4395
MabI	ACCWGGT	2 cut(s)	55, 2659
MaeI	CTAG	7 cut(s)	699, 1308, 1634, 2387, 3003, 3884, 4187
MaeII	ACGT	2 cut(s)	549, 1470
MbiI	CCGCTC	3 cut(s)	1250, 1565, 2450
MfeI	CAATTG	3 cut(s)	714, 1044, 4152
MflI	RGATCY	8 cut(s)	1753, 1798, 2129, 2788, 3095, 3129, 3562, 4302
MhlI	GDGCHC	2 cut(s)	3075, 3580
MlsI	TGGCCA	3 cut(s)	1977, 3764, 4158
MluNI	TGGCCA	3 cut(s)	1977, 3764, 4158
MmeI	TCCRAC	7 cut(s)	159, 306, 365, 1884, 2599, 3029, 3681
Mox20I	TGGCCA	3 cut(s)	1977, 3764, 4158
MroXI	GAANNNNTTC	2 cut(s)	2295, 3821
MscI	TGGCCA	3 cut(s)	1977, 3764, 4158
MseI	TTAA	8 cut(s)	309, 318, 2313, 2577, 2643, 2810, 3375, 3456
MslI	CAYNNNNRTG	2 cut(s)	906, 3726
Msp20I	TGGCCA	3 cut(s)	1977, 3764, 4158
MspA1I	CMGCKG	7 cut(s)	758, 983, 2051, 2914, 2941, 3587, 3788
MspI	CCGG	9 cut(s)	303, 359, 605, 620, 859, 1032, 3593, 3658, 4395
MunI	CAATTG	3 cut(s)	714, 1044, 4152
Mva1269I	GAATGC	3 cut(s)	1657, 3295, 3976
MvaI	CCWGG	9 cut(s)	57, 406, 1575, 2134, 2592, 2661, 3665, 4160, 4214
NciI	CCSGG	3 cut(s)	605, 860, 4395
NdeI	CATATG	2 cut(s)	2727, 4108
NlaIII	CATG	8 cut(s)	116, 734, 1064, 1544, 1556, 1955, 2956, 3725
NmeAIII	GCCGAG	1 cut(s)	1646
NspI	RCATGY	2 cut(s)	734, 1064
NspV	TTCGAA	1 cut(s)	563
PaeR7I	CTCGAG	1 cut(s)	1328
PagI	TCATGA	1 cut(s)	1951
PaqCI	CACCTGC	1 cut(s)	2510
PasI	CCCWGGG	1 cut(s)	4213
PciSI	GCTCTTC	1 cut(s)	2884
PctI	GAATGC	3 cut(s)	1657, 3295, 3976
PdmI	GAANNNNTTC	2 cut(s)	2295, 3821
Pfl23II	CGTACG	1 cut(s)	3429
PfoI	TCCNGGA	1 cut(s)	3663
PinAI	ACCGGT	1 cut(s)	3657
PpuMI	RGGWCCY	1 cut(s)	2169
PshBI	ATTAAT	1 cut(s)	2577
Psp1406I	AACGTT	1 cut(s)	1470
Psp5II	RGGWCCY	1 cut(s)	2169
Psp6I	CCWGG	9 cut(s)	55, 404, 1573, 2132, 2590, 2659, 3663, 4158, 4212
PspEI	GGTNACC	1 cut(s)	2981
PspFI	CCCAGC	2 cut(s)	2159, 3714
PspGI	CCWGG	9 cut(s)	55, 404, 1573, 2132, 2590, 2659, 3663, 4158, 4212
PspLI	CGTACG	1 cut(s)	3429
PspPI	GGNCC	9 cut(s)	1691, 2169, 2588, 2834, 2897, 3391, 3703, 3718, 4210
PspPPI	RGGWCCY	1 cut(s)	2169
PstNI	CAGNNNCTG	6 cut(s)	1226, 1505, 1922, 1940, 3587, 3788
PsuI	RGATCY	8 cut(s)	1753, 1798, 2129, 2788, 3095, 3129, 3562, 4302
PvuII	CAGCTG	5 cut(s)	758, 983, 2051, 3587, 3788
RsaI	GTAC	7 cut(s)	83, 1539, 3431, 3656, 3734, 4075, 4081
RsaNI	GTAC	7 cut(s)	82, 1538, 3430, 3655, 3733, 4074, 4080
RseI	CAYNNNNRTG	2 cut(s)	906, 3726
SapI	GCTCTTC	1 cut(s)	2884
SaqAI	TTAA	8 cut(s)	309, 318, 2313, 2577, 2643, 2810, 3375, 3456
Sau96I	GGNCC	9 cut(s)	1691, 2169, 2588, 2834, 2897, 3391, 3703, 3718, 4210
ScaI	AGTACT	2 cut(s)	83, 4075
SduI	GDGCHC	2 cut(s)	3075, 3580
SexAI	ACCWGGT	2 cut(s)	55, 2659
SfaNI	GCATC	3 cut(s)	1831, 3130, 4395
SfcI	CTRYAG	2 cut(s)	654, 2349
Sfr274I	CTCGAG	1 cut(s)	1328
SfuI	TTCGAA	1 cut(s)	563
SinI	GGWCC	7 cut(s)	1691, 2169, 2588, 2834, 2897, 3703, 3718
SlaI	CTCGAG	1 cut(s)	1328
SmiMI	CAYNNNNRTG	2 cut(s)	906, 3726
SmlI	CTYRAG	5 cut(s)	1328, 1531, 2263, 2320, 3383
SmoI	CTYRAG	5 cut(s)	1328, 1531, 2263, 2320, 3383
SpeI	ACTAGT	1 cut(s)	698
SspI	AATATT	1 cut(s)	1235
SspMI	CTAG	7 cut(s)	699, 1308, 1634, 2387, 3003, 3884, 4187
StyI	CCWWGG	2 cut(s)	366, 3709
TaiI	ACGT	2 cut(s)	552, 1473
TaqII	GACCGA	1 cut(s)	2169
TatI	WGTACW	3 cut(s)	81, 1537, 4073
TauI	GCSGC	4 cut(s)	358, 1568, 3622, 4132
Tru1I	TTAA	8 cut(s)	309, 318, 2313, 2577, 2643, 2810, 3375, 3456
Tru9I	TTAA	8 cut(s)	309, 318, 2313, 2577, 2643, 2810, 3375, 3456
TscAI	CASTG	9 cut(s)	615, 1227, 1531, 2017, 2367, 3702, 4021, 4300, 4408
TspGWI	ACGGA	3 cut(s)	530, 2003, 3357
TspRI	CASTG	9 cut(s)	615, 1227, 1531, 2017, 2367, 3702, 4021, 4300, 4408
VpaK11BI	GGWCC	7 cut(s)	1691, 2169, 2588, 2834, 2897, 3703, 3718
VspI	ATTAAT	1 cut(s)	2577
XagI	CCTNNNNNAGG	2 cut(s)	3386, 3958
XapI	RAATTY	9 cut(s)	140, 329, 495, 559, 1696, 2420, 2545, 2703, 4222
XceI	RCATGY	2 cut(s)	734, 1064
XcmI	CCANNNNNNNNNTGG	1 cut(s)	373
XhoI	CTCGAG	1 cut(s)	1328
XmnI	GAANNNNTTC	2 cut(s)	2295, 3821
XspI	CTAG	7 cut(s)	699, 1308, 1634, 2387, 3003, 3884, 4187
ZrmI	AGTACT	2 cut(s)	83, 4075

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.