MD03G1296300.v1.1
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001630
Physical Location & Seq
Forward (+)
37208462 .. 37210624
2163 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1296300.v1.1.491

Sequence Viewer

Length: 582 bp
ATGTTTCTGTTCGACTGGTTCTACGGTGTGCTCGCGTCCCTCGGTCTCTGGCAAAAAGAGGCCAAGATCTTGTTCCTCGGCCTCGATAACGCCGGCAAGACCACCTTGCTCCACATGTTGAAAGACGAGAGGCTGGTGCAGCATCAGCCCACGCAGTATCCAACATCGGAGGAGTTGAGCATTGGGAAAATCAAGTTCAAGGCCTTTGATTTGGGAGGCCATCAGATTGCTCGCAGAGTTTGGAGGGATTACTATGCAAAGGTAGATGCTGTGGTGTACCTGGTGGATGCCTCTGACAAGGAGCGGTTTGCAGAATCAAAAAAGGAACTAGATGCGCTCCTTTCGGATGAAGCCCTAGCCACTGTTCCATTTCTAATTCTAGGGAACAAGATTGACCTACCATATGCTGCCTCAGAGGATGAGTTGCGGTATCACCTTGGGCTGACCAACTTCACCACTGGCAAGGGGAAGGTAAACCTAACAGACTCAAATGTCCGTCCATTAGAAGTATTTATGTGCAGCATTGTACGGAAAATGGGGTACGGTGATGGTTTCAAATGGCTCTCTCAATACATTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

194

Amino Acids

22.04

Weight (kDa)

6.91

Isoelectric Point (pI)

25.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 192 1.4e-64 ADP-ribosylation factor family
SRPRB PF09439 19 - 144 3.9e-08 Signal recognition particle receptor beta subunit
Roc PF08477 22 - 132 2.8e-08 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 22 - 130 3.4e-07 50S ribosome-binding GTPase
Ras PF00071 22 - 134 3.1e-07 Ras family
G-alpha PF00503 51 - 133 7.3e-08 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 491
AccBSI CCGCTC 1 cut(s) 304
AccII CGCG 1 cut(s) 35
AciI CCGC 2 cut(s) 304, 427
AfaI GTAC 3 cut(s) 278, 528, 542
AflIII ACRYGT 1 cut(s) 114
AgsI TTSAA 3 cut(s) 121, 199, 556
AjnI CCWGG 1 cut(s) 279
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 1 cut(s) 50
AoxI GGCC 4 cut(s) 60, 79, 201, 217
ApeKI GCWGC 3 cut(s) 139, 407, 519
AspLEI GCGC 1 cut(s) 337
AsuHPI GGTGA 3 cut(s) 425, 445, 557
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 3 cut(s) 151, 394, 531
BccI CCATC 2 cut(s) 228, 542
BciT130I CCWGG 1 cut(s) 281
BciVI GTATCC 1 cut(s) 168
BcoDI GTCTC 1 cut(s) 50
BfaI CTAG 3 cut(s) 329, 356, 380
BfuI GTATCC 1 cut(s) 168
BglII AGATCT 1 cut(s) 66
BisI GCNGC 3 cut(s) 140, 408, 520
BlsI GCNGC 3 cut(s) 141, 409, 521
Bme1390I CCNGG 1 cut(s) 281
BmrFI CCNGG 1 cut(s) 281
BmsI GCATC 4 cut(s) 151, 256, 277, 322
BsaI GGTCTC 1 cut(s) 50
BsaJI CCNNGG 3 cut(s) 40, 76, 436
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 2 cut(s) 20, 463
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 3 cut(s) 40, 76, 436
BseGI GGATG 3 cut(s) 292, 352, 424
BseMII CTCAG 1 cut(s) 426
BseNI ACTGG 2 cut(s) 20, 463
BseRI GAGGAG 1 cut(s) 185
BseXI GCAGC 3 cut(s) 151, 394, 531
BsgI GTGCAG 2 cut(s) 158, 538
Bsh1236I CGCG 1 cut(s) 35
BshFI GGCC 4 cut(s) 62, 81, 203, 219
BsiHKAI GWGCWC 1 cut(s) 33
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 1 cut(s) 22
BsmAI GTCTC 1 cut(s) 50
BsmFI GGGAC 1 cut(s) 22
BsnI GGCC 4 cut(s) 62, 81, 203, 219
Bso31I GGTCTC 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 1 cut(s) 66
BspACI CCGC 2 cut(s) 304, 427
BspANI GGCC 4 cut(s) 62, 81, 203, 219
BspCNI CTCAG 1 cut(s) 425
BspFNI CGCG 1 cut(s) 35
BspTNI GGTCTC 1 cut(s) 50
BsrBI CCGCTC 1 cut(s) 304
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 2 cut(s) 20, 463
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 3 cut(s) 40, 76, 436
BssMI GATC 1 cut(s) 66
BssT1I CCWWGG 1 cut(s) 436
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 3 cut(s) 26, 364, 545
BstC8I GCNNGC 3 cut(s) 33, 94, 232
BstDEI CTNAG 1 cut(s) 412
BstF5I GGATG 3 cut(s) 292, 352, 424
BstFNI CGCG 1 cut(s) 35
BstHHI GCGC 1 cut(s) 337
BstKTI GATC 1 cut(s) 69
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 1 cut(s) 66
BstMWI GCNNNNNNNGC 2 cut(s) 139, 145
BstNI CCWGG 1 cut(s) 281
BstNSI RCATGY 1 cut(s) 118
BstSCI CCNGG 1 cut(s) 279
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 3 cut(s) 151, 394, 531
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
BsuI GTATCC 1 cut(s) 168
BsuRI GGCC 4 cut(s) 62, 81, 203, 219
BtsCI GGATG 3 cut(s) 292, 352, 424
BtsIMutI CAGTG 2 cut(s) 360, 456
Cac8I GCNNGC 3 cut(s) 33, 94, 232
CfoI GCGC 1 cut(s) 337
Cfr10I RCCGGY 1 cut(s) 92
CseI GACGC 1 cut(s) 24
CsiI ACCWGGT 1 cut(s) 279
Csp6I GTAC 3 cut(s) 277, 527, 541
CviAII CATG 1 cut(s) 115
CviQI GTAC 3 cut(s) 277, 527, 541
DdeI CTNAG 1 cut(s) 412
DpnI GATC 1 cut(s) 68
DpnII GATC 1 cut(s) 66
DrdI GACNNNNNNGTC 1 cut(s) 491
DseDI GACNNNNNNGTC 1 cut(s) 491
Eco130I CCWWGG 1 cut(s) 436
Eco147I AGGCCT 1 cut(s) 203
Eco31I GGTCTC 1 cut(s) 50
EcoRII CCWGG 1 cut(s) 279
EcoT14I CCWWGG 1 cut(s) 436
ErhI CCWWGG 1 cut(s) 436
FaeI CATG 1 cut(s) 118
FaiI YATR 5 cut(s) 116, 255, 403, 405, 515
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FaqI GGGAC 1 cut(s) 22
FatI CATG 1 cut(s) 114
FauNDI CATATG 1 cut(s) 403
Fnu4HI GCNGC 3 cut(s) 140, 408, 520
FokI GGATG 3 cut(s) 299, 359, 431
Fsp4HI GCNGC 3 cut(s) 140, 408, 520
FspBI CTAG 3 cut(s) 329, 356, 380
GlaI GCGC 1 cut(s) 336
GluI GCNGC 3 cut(s) 140, 408, 520
HaeIII GGCC 4 cut(s) 62, 81, 203, 219
HapII CCGG 1 cut(s) 93
HgaI GACGC 1 cut(s) 24
HhaI GCGC 1 cut(s) 337
Hin1II CATG 1 cut(s) 118
Hin6I GCGC 1 cut(s) 335
HinP1I GCGC 1 cut(s) 335
HinfI GANTC 2 cut(s) 314, 485
HpaII CCGG 1 cut(s) 93
HphI GGTGA 3 cut(s) 425, 445, 557
Hpy166II GTNNAC 2 cut(s) 277, 475
Hpy188I TCNGA 5 cut(s) 169, 225, 295, 346, 415
Hpy8I GTNNAC 2 cut(s) 277, 475
HpyAV CCTTC 1 cut(s) 463
HpyCH4III ACNGT 3 cut(s) 26, 364, 545
HpyCH4V TGCA 4 cut(s) 139, 257, 311, 519
HpyF10VI GCNNNNNNNGC 2 cut(s) 139, 145
HpyF3I CTNAG 1 cut(s) 412
Hsp92II CATG 1 cut(s) 118
HspAI GCGC 1 cut(s) 335
KroI GCCGGC 1 cut(s) 92
KroNI GCCGGC 1 cut(s) 94
Kzo9I GATC 1 cut(s) 66
LmnI GCTCC 3 cut(s) 114, 301, 342
LpnPI CCDG 6 cut(s) 34, 106, 119, 266, 293, 444
Lsp1109I GCAGC 3 cut(s) 151, 394, 531
LweI GCATC 4 cut(s) 151, 256, 277, 322
MabI ACCWGGT 1 cut(s) 279
MaeI CTAG 3 cut(s) 329, 356, 380
MalI GATC 1 cut(s) 68
MbiI CCGCTC 1 cut(s) 304
MboI GATC 1 cut(s) 66
MflI RGATCY 1 cut(s) 66
MhlI GDGCHC 1 cut(s) 33
MluCI AATT 1 cut(s) 375
MlyI GAGTC 1 cut(s) 479
MmeI TCCRAC 1 cut(s) 185
MroNI GCCGGC 1 cut(s) 92
MseI TTAA 1 cut(s) 576
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 281
MvaI CCWGG 1 cut(s) 281
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 2 cut(s) 139, 145
NaeI GCCGGC 1 cut(s) 94
NdeI CATATG 1 cut(s) 403
NdeII GATC 1 cut(s) 66
NgoMIV GCCGGC 1 cut(s) 92
NlaIII CATG 1 cut(s) 118
NmeAIII GCCGAG 1 cut(s) 57
NspI RCATGY 1 cut(s) 118
PceI AGGCCT 1 cut(s) 203
PciI ACATGT 1 cut(s) 114
PdiI GCCGGC 1 cut(s) 94
PfeI GAWTC 1 cut(s) 314
PkrI GCNGC 3 cut(s) 141, 409, 521
PleI GAGTC 1 cut(s) 479
PpsI GAGTC 1 cut(s) 479
PscI ACATGT 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PsuI RGATCY 1 cut(s) 66
RsaI GTAC 3 cut(s) 278, 528, 542
RsaNI GTAC 3 cut(s) 277, 527, 541
SaqAI TTAA 1 cut(s) 576
SatI GCNGC 3 cut(s) 140, 408, 520
Sau3AI GATC 1 cut(s) 66
SchI GAGTC 1 cut(s) 479
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 1 cut(s) 33
SetI ASST 7 cut(s) 107, 264, 282, 399, 438, 474, 480
SexAI ACCWGGT 1 cut(s) 279
SfaNI GCATC 4 cut(s) 151, 256, 277, 322
Sse9I AATT 1 cut(s) 375
SseBI AGGCCT 1 cut(s) 203
SsiI CCGC 2 cut(s) 304, 427
SspMI CTAG 3 cut(s) 329, 356, 380
StuI AGGCCT 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 279
StyI CCWWGG 1 cut(s) 436
TaaI ACNGT 3 cut(s) 26, 364, 545
TaqI TCGA 2 cut(s) 12, 84
TaqII GACCGA 1 cut(s) 32
TasI AATT 1 cut(s) 375
TfiI GAWTC 1 cut(s) 314
Tru1I TTAA 1 cut(s) 576
Tru9I TTAA 1 cut(s) 576
TscAI CASTG 2 cut(s) 367, 463
TseI GCWGC 3 cut(s) 139, 407, 519
TspDTI ATGAA 1 cut(s) 363
TspGWI ACGGA 2 cut(s) 485, 544
TspRI CASTG 2 cut(s) 367, 463
XceI RCATGY 1 cut(s) 118
XspI CTAG 3 cut(s) 329, 356, 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.