pycom11g27560
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001630
Physical Location & Seq
Forward (+)
29769280 .. 29770980
1701 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g27560.3

Sequence Viewer

Length: 582 bp
ATGTTTCTGTTCGACTGGTTCTACGGTGTGCTCGCGTCCCTCGGTCTCTGGCAGAAAGAGGCCAAGATCTTGTTCCTCGGCCTCGATAACGCCGGCAAGACCACCTTGCTCCACATGTTGAAAGACGAGAGACTGGTGCAGCACCAGCCCACGCAGTATCCAACATCGGAGGAGTTGAGCATTGGGAAAATCAAGTTCAAGGCCTTTGATTTGGGAGGTCATCAGATTGCTCGCAGAGTTTGGAGGGATTACTATGCAAAGGTGGATGCTGTGGTGTACCTGGTGGATGCCTATGACAAGGCGCGCTTTGCAGAATCCAAAAAGGAACTAGATGCACTCCTTTCGGATGAAGCCCTAGCCACTGTCCCATTTCTAATTCTAGGGAACAAGATTGACCTACCATATGCTGCCTCGGAGGATGAGTTGCGATATCACCTTGGGCTGACCAACTTCACCACTGGCAAGGGGAATGTAAACCTAACGGACTCAAGTGTCCGTCCGCTAGAAGTGTTTATGTGCAGCATTGTACGGAAAATGGGGTACGGCGATGGTTTCAAATGGCTCTCTCAATACATCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

194

Amino Acids

22.01

Weight (kDa)

6.91

Isoelectric Point (pI)

28.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 491
AccII CGCG 2 cut(s) 35, 304
AciI CCGC 1 cut(s) 500
AfaI GTAC 3 cut(s) 278, 528, 542
AflIII ACRYGT 1 cut(s) 114
AgsI TTSAA 3 cut(s) 121, 199, 556
AjnI CCWGG 1 cut(s) 279
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 2 cut(s) 50, 124
AoxI GGCC 3 cut(s) 60, 79, 201
ApeKI GCWGC 3 cut(s) 139, 407, 519
AspLEI GCGC 2 cut(s) 304, 306
AsuHPI GGTGA 2 cut(s) 425, 445
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 3 cut(s) 151, 394, 531
BccI CCATC 1 cut(s) 542
BceAI ACGGC 1 cut(s) 559
BcgI CGANNNNNNTGC 2 cut(s) 324, 358
BciT130I CCWGG 1 cut(s) 281
BciVI GTATCC 1 cut(s) 168
BcoDI GTCTC 2 cut(s) 50, 124
BfaI CTAG 4 cut(s) 329, 356, 380, 503
BfuI GTATCC 1 cut(s) 168
BglII AGATCT 1 cut(s) 66
BisI GCNGC 3 cut(s) 140, 408, 520
BlsI GCNGC 3 cut(s) 141, 409, 521
Bme1390I CCNGG 1 cut(s) 281
BmrFI CCNGG 1 cut(s) 281
BmsI GCATC 3 cut(s) 256, 277, 322
BpuEI CTTGAG 1 cut(s) 472
BsaI GGTCTC 1 cut(s) 50
BsaJI CCNNGG 4 cut(s) 40, 76, 411, 436
BsaXI ACNNNNNCTCC 2 cut(s) 407, 437
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 3 cut(s) 20, 138, 463
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 4 cut(s) 40, 76, 411, 436
BseGI GGATG 4 cut(s) 271, 292, 352, 424
BseNI ACTGG 3 cut(s) 20, 138, 463
BsePI GCGCGC 1 cut(s) 302
BseRI GAGGAG 1 cut(s) 185
BseXI GCAGC 3 cut(s) 151, 394, 531
BsgI GTGCAG 2 cut(s) 158, 538
Bsh1236I CGCG 2 cut(s) 35, 304
BshFI GGCC 3 cut(s) 62, 81, 203
BsiHKAI GWGCWC 1 cut(s) 33
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 2 cut(s) 22, 350
BsmAI GTCTC 2 cut(s) 50, 124
BsmFI GGGAC 2 cut(s) 22, 350
BsnI GGCC 3 cut(s) 62, 81, 203
Bso31I GGTCTC 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 1 cut(s) 66
BspACI CCGC 1 cut(s) 500
BspANI GGCC 3 cut(s) 62, 81, 203
BspFNI CGCG 2 cut(s) 35, 304
BspTNI GGTCTC 1 cut(s) 50
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 3 cut(s) 20, 138, 463
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 4 cut(s) 40, 76, 411, 436
BssHII GCGCGC 1 cut(s) 302
BssMI GATC 1 cut(s) 66
BssT1I CCWWGG 1 cut(s) 436
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 2 cut(s) 26, 364
BstC8I GCNNGC 4 cut(s) 33, 94, 232, 304
BstF5I GGATG 4 cut(s) 271, 292, 352, 424
BstFNI CGCG 2 cut(s) 35, 304
BstHHI GCGC 2 cut(s) 304, 306
BstKTI GATC 1 cut(s) 69
BstMAI GTCTC 2 cut(s) 50, 124
BstMBI GATC 1 cut(s) 66
BstMWI GCNNNNNNNGC 2 cut(s) 145, 308
BstNI CCWGG 1 cut(s) 281
BstNSI RCATGY 1 cut(s) 118
BstSCI CCNGG 1 cut(s) 279
BstUI CGCG 2 cut(s) 35, 304
BstV1I GCAGC 3 cut(s) 151, 394, 531
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
BsuI GTATCC 1 cut(s) 168
BsuRI GGCC 3 cut(s) 62, 81, 203
BtgZI GCGATG 1 cut(s) 561
BtsCI GGATG 4 cut(s) 271, 292, 352, 424
BtsIMutI CAGTG 2 cut(s) 360, 456
Cac8I GCNNGC 4 cut(s) 33, 94, 232, 304
CfoI GCGC 2 cut(s) 304, 306
Cfr10I RCCGGY 1 cut(s) 92
CseI GACGC 1 cut(s) 24
CsiI ACCWGGT 1 cut(s) 279
Csp6I GTAC 3 cut(s) 277, 527, 541
CviAII CATG 1 cut(s) 115
CviJI RGCY 8 cut(s) 62, 81, 148, 203, 353, 359, 442, 562
CviKI_1 RGCY 8 cut(s) 62, 81, 148, 203, 353, 359, 442, 562
CviQI GTAC 3 cut(s) 277, 527, 541
DpnI GATC 1 cut(s) 68
DpnII GATC 1 cut(s) 66
DrdI GACNNNNNNGTC 1 cut(s) 491
DseDI GACNNNNNNGTC 1 cut(s) 491
Eco130I CCWWGG 1 cut(s) 436
Eco147I AGGCCT 1 cut(s) 203
Eco31I GGTCTC 1 cut(s) 50
Eco32I GATATC 1 cut(s) 431
EcoRII CCWGG 1 cut(s) 279
EcoRV GATATC 1 cut(s) 431
EcoT14I CCWWGG 1 cut(s) 436
ErhI CCWWGG 1 cut(s) 436
FaeI CATG 1 cut(s) 118
FaiI YATR 6 cut(s) 116, 255, 294, 403, 405, 515
FalI AAGNNNNNCTT 4 cut(s) 89, 121, 290, 322
FaqI GGGAC 2 cut(s) 22, 350
FatI CATG 1 cut(s) 114
FauNDI CATATG 1 cut(s) 403
Fnu4HI GCNGC 3 cut(s) 140, 408, 520
FokI GGATG 4 cut(s) 278, 299, 359, 431
Fsp4HI GCNGC 3 cut(s) 140, 408, 520
FspBI CTAG 4 cut(s) 329, 356, 380, 503
GlaI GCGC 2 cut(s) 303, 305
GluI GCNGC 3 cut(s) 140, 408, 520
HaeIII GGCC 3 cut(s) 62, 81, 203
HapII CCGG 1 cut(s) 93
HgaI GACGC 1 cut(s) 24
HhaI GCGC 2 cut(s) 304, 306
Hin1II CATG 1 cut(s) 118
Hin6I GCGC 2 cut(s) 302, 304
HinP1I GCGC 2 cut(s) 302, 304
HinfI GANTC 2 cut(s) 314, 485
HpaII CCGG 1 cut(s) 93
HphI GGTGA 2 cut(s) 425, 445
Hpy166II GTNNAC 2 cut(s) 277, 475
Hpy188I TCNGA 4 cut(s) 169, 225, 346, 415
Hpy8I GTNNAC 2 cut(s) 277, 475
HpyCH4III ACNGT 2 cut(s) 26, 364
HpyCH4V TGCA 5 cut(s) 139, 257, 311, 335, 519
HpyF10VI GCNNNNNNNGC 2 cut(s) 145, 308
Hsp92II CATG 1 cut(s) 118
HspAI GCGC 2 cut(s) 302, 304
KroI GCCGGC 1 cut(s) 92
KroNI GCCGGC 1 cut(s) 94
Kzo9I GATC 1 cut(s) 66
LmnI GCTCC 1 cut(s) 114
LpnPI CCDG 7 cut(s) 34, 106, 119, 158, 266, 293, 444
Lsp1109I GCAGC 3 cut(s) 151, 394, 531
LweI GCATC 3 cut(s) 256, 277, 322
MabI ACCWGGT 1 cut(s) 279
MaeI CTAG 4 cut(s) 329, 356, 380, 503
MalI GATC 1 cut(s) 68
MboI GATC 1 cut(s) 66
MflI RGATCY 1 cut(s) 66
MhlI GDGCHC 1 cut(s) 33
MluCI AATT 1 cut(s) 375
MlyI GAGTC 1 cut(s) 479
MmeI TCCRAC 1 cut(s) 185
MnlI CCTC 9 cut(s) 50, 52, 86, 92, 163, 209, 237, 409, 421
MroNI GCCGGC 1 cut(s) 92
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 281
MvaI CCWGG 1 cut(s) 281
MvnI CGCG 2 cut(s) 35, 304
MwoI GCNNNNNNNGC 2 cut(s) 145, 308
NaeI GCCGGC 1 cut(s) 94
NdeI CATATG 1 cut(s) 403
NdeII GATC 1 cut(s) 66
NgoMIV GCCGGC 1 cut(s) 92
NlaIII CATG 1 cut(s) 118
NmeAIII GCCGAG 1 cut(s) 57
NspI RCATGY 1 cut(s) 118
PauI GCGCGC 1 cut(s) 302
PceI AGGCCT 1 cut(s) 203
PciI ACATGT 1 cut(s) 114
PdiI GCCGGC 1 cut(s) 94
PfeI GAWTC 1 cut(s) 314
PkrI GCNGC 3 cut(s) 141, 409, 521
PleI GAGTC 1 cut(s) 479
PpsI GAGTC 1 cut(s) 479
PscI ACATGT 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PsuI RGATCY 1 cut(s) 66
PteI GCGCGC 1 cut(s) 302
RsaI GTAC 3 cut(s) 278, 528, 542
RsaNI GTAC 3 cut(s) 277, 527, 541
SatI GCNGC 3 cut(s) 140, 408, 520
Sau3AI GATC 1 cut(s) 66
SchI GAGTC 1 cut(s) 479
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 1 cut(s) 33
SetI ASST 7 cut(s) 107, 220, 264, 282, 399, 438, 480
SexAI ACCWGGT 1 cut(s) 279
SfaNI GCATC 3 cut(s) 256, 277, 322
SmlI CTYRAG 1 cut(s) 487
SmoI CTYRAG 1 cut(s) 487
Sse9I AATT 1 cut(s) 375
SseBI AGGCCT 1 cut(s) 203
SsiI CCGC 1 cut(s) 500
SspMI CTAG 4 cut(s) 329, 356, 380, 503
StuI AGGCCT 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 279
StyI CCWWGG 1 cut(s) 436
TaaI ACNGT 2 cut(s) 26, 364
TaqI TCGA 2 cut(s) 12, 84
TaqII GACCGA 1 cut(s) 32
TasI AATT 1 cut(s) 375
TfiI GAWTC 1 cut(s) 314
TscAI CASTG 2 cut(s) 367, 463
TseI GCWGC 3 cut(s) 139, 407, 519
TspDTI ATGAA 1 cut(s) 363
TspGWI ACGGA 3 cut(s) 485, 497, 544
TspRI CASTG 2 cut(s) 367, 463
XceI RCATGY 1 cut(s) 118
XspI CTAG 4 cut(s) 329, 356, 380, 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.