RchiOBHm_Chr3g0456901
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001630
Physical Location & Seq
Reverse (-)
6228098 .. 6231397
3300 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42367

Sequence Viewer

Length: 582 bp
ATGTTCTTGTTCGATTGGTTCTATGGGGTTCTCGCGTCGCTAGGCTTGTGGCAGAAGGAGGCCAAGATCTTGTTCTTGGGTCTCGATAATGCCGGCAAAACCACCTTGCTTCATATGCTTAAAGACGAGAGATTAGTTCAGCACCAACCGACTCAGTATCCGACATCTGAGGAGTTGAGCATTGGGAAAATCAAGTTCAAGGCTTTTGATTTGGGTGGGCATCAGATTGCTCGCAGAGTTTGGAAAGACTACTATGCCAAGGTGGATGCAGTTGTTTACCTAGTGGATTCTTATGACAAGGAAAGATTCGCGGAATCAAAAAAGGAGCTGGATGCTCTCCTCTCTGATGAGGCATTGGCCACTGTTCCATTCCTTATTCTGGGAAACAAGATTGACATACCATATGCGGCATCAGAAGAAGAGTTGCGATATCACCTTGGTCTGAACAACTTCACCACAGGCAAGGGTAAAGTAAACTTGGCAGACTCGAACGTGCGCCCTCTTGAAGTATTCATGTGCAGCATTGTTCGCAAAATGGGTTATGGAGATGGATTCAAGTGGGTTTCTCAGTACATCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

193

Amino Acids

22.08

Weight (kDa)

6.91

Isoelectric Point (pI)

28.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 192 3.7e-64 ADP-ribosylation factor family
SRPRB PF09439 19 - 145 5.4e-09 Signal recognition particle receptor beta subunit
Roc PF08477 22 - 132 2.2e-08 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 22 - 130 4.1e-07 50S ribosome-binding GTPase
Ras PF00071 22 - 134 1.6e-06 Ras family
G-alpha PF00503 50 - 133 7.8e-09 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 35, 311
AciI CCGC 2 cut(s) 311, 407
AcoI YGGCCR 1 cut(s) 357
AfaI GTAC 1 cut(s) 572
AfiI CCNNNNNNNGG 1 cut(s) 379
AgsI TTSAA 3 cut(s) 199, 506, 556
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AluBI AGCT 1 cut(s) 328
AluI AGCT 1 cut(s) 328
Alw26I GTCTC 1 cut(s) 86
AoxI GGCC 2 cut(s) 60, 357
ApeKI GCWGC 1 cut(s) 519
Asp700I GAANNNNTTC 1 cut(s) 449
AspLEI GCGC 1 cut(s) 498
AsuHPI GGTGA 2 cut(s) 425, 445
BalI TGGCCA 1 cut(s) 359
BbvI GCAGC 1 cut(s) 531
BccI CCATC 1 cut(s) 542
BciVI GTATCC 1 cut(s) 168
BcoDI GTCTC 1 cut(s) 86
BfaI CTAG 2 cut(s) 41, 281
BfuI GTATCC 1 cut(s) 168
BglII AGATCT 1 cut(s) 66
BisI GCNGC 2 cut(s) 408, 520
BlsI GCNGC 2 cut(s) 409, 521
BmsI GCATC 4 cut(s) 229, 256, 322, 419
BsaI GGTCTC 1 cut(s) 86
BsaJI CCNNGG 2 cut(s) 258, 436
Bsc4I CCNNNNNNNGG 1 cut(s) 379
Bse118I RCCGGY 1 cut(s) 92
BseDI CCNNGG 2 cut(s) 258, 436
BseGI GGATG 2 cut(s) 271, 337
BseLI CCNNNNNNNGG 1 cut(s) 379
BseMII CTCAG 3 cut(s) 159, 167, 581
BseRI GAGGAG 2 cut(s) 185, 329
BseXI GCAGC 1 cut(s) 531
BsgI GTGCAG 1 cut(s) 538
Bsh1236I CGCG 2 cut(s) 35, 311
BshFI GGCC 2 cut(s) 62, 359
BsiSI CCGG 1 cut(s) 93
BslI CCNNNNNNNGG 1 cut(s) 379
BsmAI GTCTC 1 cut(s) 86
BsnI GGCC 2 cut(s) 62, 359
Bso31I GGTCTC 1 cut(s) 86
Bsp143I GATC 1 cut(s) 66
BspACI CCGC 2 cut(s) 311, 407
BspANI GGCC 2 cut(s) 62, 359
BspCNI CTCAG 3 cut(s) 160, 166, 580
BspFNI CGCG 2 cut(s) 35, 311
BspTNI GGTCTC 1 cut(s) 86
BsrFI RCCGGY 1 cut(s) 92
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 2 cut(s) 258, 436
BssMI GATC 1 cut(s) 66
BssT1I CCWWGG 2 cut(s) 258, 436
Bst4CI ACNGT 1 cut(s) 364
Bst6I CTCTTC 1 cut(s) 414
BstC8I GCNNGC 2 cut(s) 94, 232
BstDEI CTNAG 3 cut(s) 153, 168, 567
BstF5I GGATG 2 cut(s) 271, 337
BstFNI CGCG 2 cut(s) 35, 311
BstHHI GCGC 1 cut(s) 498
BstKTI GATC 1 cut(s) 69
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 1 cut(s) 66
BstMWI GCNNNNNNNGC 2 cut(s) 115, 528
BstUI CGCG 2 cut(s) 35, 311
BstV1I GCAGC 1 cut(s) 531
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
BsuI GTATCC 1 cut(s) 168
BsuRI GGCC 2 cut(s) 62, 359
BtsCI GGATG 2 cut(s) 271, 337
BtsIMutI CAGTG 1 cut(s) 360
Cac8I GCNNGC 2 cut(s) 94, 232
CfoI GCGC 1 cut(s) 498
Cfr10I RCCGGY 1 cut(s) 92
CseI GACGC 1 cut(s) 24
Csp6I GTAC 1 cut(s) 571
CviAII CATG 1 cut(s) 514
CviJI RGCY 5 cut(s) 45, 62, 203, 328, 359
CviKI_1 RGCY 5 cut(s) 45, 62, 203, 328, 359
CviQI GTAC 1 cut(s) 571
DdeI CTNAG 3 cut(s) 153, 168, 567
DpnI GATC 1 cut(s) 68
DpnII GATC 1 cut(s) 66
EaeI YGGCCR 1 cut(s) 357
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
Eco130I CCWWGG 2 cut(s) 258, 436
Eco31I GGTCTC 1 cut(s) 86
Eco32I GATATC 1 cut(s) 431
EcoRV GATATC 1 cut(s) 431
EcoT14I CCWWGG 2 cut(s) 258, 436
ErhI CCWWGG 2 cut(s) 258, 436
FaeI CATG 1 cut(s) 517
FatI CATG 1 cut(s) 513
FauNDI CATATG 2 cut(s) 114, 403
Fnu4HI GCNGC 2 cut(s) 408, 520
FokI GGATG 2 cut(s) 278, 344
Fsp4HI GCNGC 2 cut(s) 408, 520
FspBI CTAG 2 cut(s) 41, 281
GlaI GCGC 1 cut(s) 497
GluI GCNGC 2 cut(s) 408, 520
HaeIII GGCC 2 cut(s) 62, 359
HapII CCGG 1 cut(s) 93
HgaI GACGC 1 cut(s) 24
HhaI GCGC 1 cut(s) 498
Hin1II CATG 1 cut(s) 517
Hin6I GCGC 1 cut(s) 496
HinP1I GCGC 1 cut(s) 496
HinfI GANTC 6 cut(s) 151, 287, 306, 314, 485, 552
HpaII CCGG 1 cut(s) 93
HphI GGTGA 2 cut(s) 425, 445
Hpy166II GTNNAC 2 cut(s) 277, 475
Hpy188I TCNGA 6 cut(s) 162, 169, 225, 346, 415, 444
Hpy188III TCNNGA 2 cut(s) 83, 503
Hpy8I GTNNAC 2 cut(s) 277, 475
Hpy99I CGWCG 1 cut(s) 40
HpyAV CCTTC 1 cut(s) 49
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 2 cut(s) 269, 519
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 528
HpyF3I CTNAG 3 cut(s) 153, 168, 567
HpySE526I ACGT 1 cut(s) 492
Hsp92II CATG 1 cut(s) 517
HspAI GCGC 1 cut(s) 496
KroI GCCGGC 1 cut(s) 92
KroNI GCCGGC 1 cut(s) 94
Kzo9I GATC 1 cut(s) 66
LmnI GCTCC 1 cut(s) 325
LpnPI CCDG 4 cut(s) 106, 314, 365, 444
Lsp1109I GCAGC 1 cut(s) 531
LweI GCATC 4 cut(s) 229, 256, 322, 419
MaeI CTAG 2 cut(s) 41, 281
MaeII ACGT 1 cut(s) 492
MalI GATC 1 cut(s) 68
MboI GATC 1 cut(s) 66
MboII GAAGA 2 cut(s) 428, 431
MflI RGATCY 1 cut(s) 66
MlsI TGGCCA 1 cut(s) 359
MluNI TGGCCA 1 cut(s) 359
MlyI GAGTC 2 cut(s) 145, 479
MmeI TCCRAC 1 cut(s) 185
MnlI CCTC 5 cut(s) 52, 163, 343, 350, 510
Mox20I TGGCCA 1 cut(s) 359
MroNI GCCGGC 1 cut(s) 92
MroXI GAANNNNTTC 1 cut(s) 449
MscI TGGCCA 1 cut(s) 359
MseI TTAA 1 cut(s) 120
Msp20I TGGCCA 1 cut(s) 359
MspI CCGG 1 cut(s) 93
MvnI CGCG 2 cut(s) 35, 311
MwoI GCNNNNNNNGC 2 cut(s) 115, 528
NaeI GCCGGC 1 cut(s) 94
NdeI CATATG 2 cut(s) 114, 403
NdeII GATC 1 cut(s) 66
NgoMIV GCCGGC 1 cut(s) 92
NlaIII CATG 1 cut(s) 517
PdiI GCCGGC 1 cut(s) 94
PdmI GAANNNNTTC 1 cut(s) 449
PfeI GAWTC 4 cut(s) 287, 306, 314, 552
PkrI GCNGC 2 cut(s) 409, 521
PleI GAGTC 2 cut(s) 145, 479
PpsI GAGTC 2 cut(s) 145, 479
PsuI RGATCY 1 cut(s) 66
RsaI GTAC 1 cut(s) 572
RsaNI GTAC 1 cut(s) 571
SaqAI TTAA 1 cut(s) 120
SatI GCNGC 2 cut(s) 408, 520
Sau3AI GATC 1 cut(s) 66
SchI GAGTC 2 cut(s) 145, 479
SetI ASST 6 cut(s) 107, 264, 282, 330, 438, 495
SfaNI GCATC 4 cut(s) 229, 256, 322, 419
SsiI CCGC 2 cut(s) 311, 407
SspMI CTAG 2 cut(s) 41, 281
StyI CCWWGG 2 cut(s) 258, 436
TaaI ACNGT 1 cut(s) 364
TaiI ACGT 1 cut(s) 495
TaqI TCGA 3 cut(s) 12, 84, 488
TatI WGTACW 1 cut(s) 570
TauI GCSGC 1 cut(s) 410
TfiI GAWTC 4 cut(s) 287, 306, 314, 552
Tru1I TTAA 1 cut(s) 120
Tru9I TTAA 1 cut(s) 120
TscAI CASTG 1 cut(s) 367
TseI GCWGC 1 cut(s) 519
TspDTI ATGAA 2 cut(s) 101, 502
TspRI CASTG 1 cut(s) 367
XmnI GAANNNNTTC 1 cut(s) 449
XspI CTAG 2 cut(s) 41, 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.