RLG00000025269
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001630
Physical Location & Seq
Forward (+)
43501670 .. 43503479
1810 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025269

Sequence Viewer

Length: 516 bp
ATGTTCTTGTTCGATTGGTTCTATGGGGTTCTCGCGTCGCTAGGCTTGTGGCAGAAGGAGGCCAAGATCTTGTTCTTGGGTCTCGATAATGCCGGCAAAACCACCTTGCTTCATATGCTTAAAGACGAGTTCAAGGCTTTTGATTTGGGTGGGCATCAGATTGCTCGCAGAGTTTGGAAAGACTACTATGCCAAGGTGGATGCAGTTGTTTACCTAGTGGATTCTTATGACAAGGAAAGATTCGCGGAATCAAAAAAGGAGCTGGATGCTCTCCTCTCTGATGAGGCATTGGCCACTGTTCCATTCCTTATTCTGGGAAACAAGATTGACATACCATATGCGGCATCAGAAGAAGAGTTGCGATATCACCTTGGTCTGAACAACTTCACCACAGGCAAGGGTAAAGTAAACTTGGCAGACTCGAACGTGCGCCCTCTTGAAGTATTCATGTGCAGCATTGTTCGCAAAATGGGTTATGGAGATGGATTCAAGTGGGTTTCTCAGTACATCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

172

Amino Acids

19.55

Weight (kDa)

6.3

Isoelectric Point (pI)

28.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 43 3.6e-10 ADP-ribosylation factor family
Arf PF00025 43 - 170 3.8e-39 ADP-ribosylation factor family
G-alpha PF00503 43 - 111 5.6e-07 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 35, 245
AciI CCGC 2 cut(s) 245, 341
AcoI YGGCCR 1 cut(s) 291
AfaI GTAC 1 cut(s) 506
AfiI CCNNNNNNNGG 1 cut(s) 313
AgsI TTSAA 3 cut(s) 133, 440, 490
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AluBI AGCT 1 cut(s) 262
AluI AGCT 1 cut(s) 262
Alw26I GTCTC 1 cut(s) 86
AoxI GGCC 2 cut(s) 60, 291
ApeKI GCWGC 1 cut(s) 453
Asp700I GAANNNNTTC 1 cut(s) 383
AspLEI GCGC 1 cut(s) 432
AsuHPI GGTGA 2 cut(s) 359, 379
BalI TGGCCA 1 cut(s) 293
BbvI GCAGC 1 cut(s) 465
BccI CCATC 1 cut(s) 476
BcoDI GTCTC 1 cut(s) 86
BfaI CTAG 2 cut(s) 41, 215
BglII AGATCT 1 cut(s) 66
BisI GCNGC 2 cut(s) 342, 454
BlsI GCNGC 2 cut(s) 343, 455
BmsI GCATC 4 cut(s) 163, 190, 256, 353
BsaI GGTCTC 1 cut(s) 86
BsaJI CCNNGG 2 cut(s) 192, 370
Bsc4I CCNNNNNNNGG 1 cut(s) 313
Bse118I RCCGGY 1 cut(s) 92
BseDI CCNNGG 2 cut(s) 192, 370
BseGI GGATG 2 cut(s) 205, 271
BseLI CCNNNNNNNGG 1 cut(s) 313
BseMII CTCAG 1 cut(s) 515
BseRI GAGGAG 1 cut(s) 263
BseXI GCAGC 1 cut(s) 465
BsgI GTGCAG 1 cut(s) 472
Bsh1236I CGCG 2 cut(s) 35, 245
BshFI GGCC 2 cut(s) 62, 293
BsiSI CCGG 1 cut(s) 93
BslI CCNNNNNNNGG 1 cut(s) 313
BsmAI GTCTC 1 cut(s) 86
BsnI GGCC 2 cut(s) 62, 293
Bso31I GGTCTC 1 cut(s) 86
Bsp143I GATC 1 cut(s) 66
BspACI CCGC 2 cut(s) 245, 341
BspANI GGCC 2 cut(s) 62, 293
BspCNI CTCAG 1 cut(s) 514
BspFNI CGCG 2 cut(s) 35, 245
BspTNI GGTCTC 1 cut(s) 86
BsrFI RCCGGY 1 cut(s) 92
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 2 cut(s) 192, 370
BssMI GATC 1 cut(s) 66
BssT1I CCWWGG 2 cut(s) 192, 370
Bst4CI ACNGT 1 cut(s) 298
Bst6I CTCTTC 1 cut(s) 348
BstC8I GCNNGC 2 cut(s) 94, 166
BstDEI CTNAG 1 cut(s) 501
BstF5I GGATG 2 cut(s) 205, 271
BstFNI CGCG 2 cut(s) 35, 245
BstHHI GCGC 1 cut(s) 432
BstKTI GATC 1 cut(s) 69
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 1 cut(s) 66
BstMWI GCNNNNNNNGC 2 cut(s) 115, 462
BstUI CGCG 2 cut(s) 35, 245
BstV1I GCAGC 1 cut(s) 465
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
BsuRI GGCC 2 cut(s) 62, 293
BtsCI GGATG 2 cut(s) 205, 271
BtsIMutI CAGTG 1 cut(s) 294
Cac8I GCNNGC 2 cut(s) 94, 166
CfoI GCGC 1 cut(s) 432
Cfr10I RCCGGY 1 cut(s) 92
CseI GACGC 1 cut(s) 24
Csp6I GTAC 1 cut(s) 505
CviAII CATG 1 cut(s) 448
CviJI RGCY 5 cut(s) 45, 62, 137, 262, 293
CviKI_1 RGCY 5 cut(s) 45, 62, 137, 262, 293
CviQI GTAC 1 cut(s) 505
DdeI CTNAG 1 cut(s) 501
DpnI GATC 1 cut(s) 68
DpnII GATC 1 cut(s) 66
EaeI YGGCCR 1 cut(s) 291
Eam1104I CTCTTC 1 cut(s) 348
EarI CTCTTC 1 cut(s) 348
Eco130I CCWWGG 2 cut(s) 192, 370
Eco31I GGTCTC 1 cut(s) 86
Eco32I GATATC 1 cut(s) 365
EcoRV GATATC 1 cut(s) 365
EcoT14I CCWWGG 2 cut(s) 192, 370
ErhI CCWWGG 2 cut(s) 192, 370
FaeI CATG 1 cut(s) 451
FatI CATG 1 cut(s) 447
FauNDI CATATG 2 cut(s) 114, 337
Fnu4HI GCNGC 2 cut(s) 342, 454
FokI GGATG 2 cut(s) 212, 278
Fsp4HI GCNGC 2 cut(s) 342, 454
FspBI CTAG 2 cut(s) 41, 215
GlaI GCGC 1 cut(s) 431
GluI GCNGC 2 cut(s) 342, 454
HaeIII GGCC 2 cut(s) 62, 293
HapII CCGG 1 cut(s) 93
HgaI GACGC 1 cut(s) 24
HhaI GCGC 1 cut(s) 432
Hin1II CATG 1 cut(s) 451
Hin6I GCGC 1 cut(s) 430
HinP1I GCGC 1 cut(s) 430
HinfI GANTC 5 cut(s) 221, 240, 248, 419, 486
HpaII CCGG 1 cut(s) 93
HphI GGTGA 2 cut(s) 359, 379
Hpy166II GTNNAC 2 cut(s) 211, 409
Hpy188I TCNGA 4 cut(s) 159, 280, 349, 378
Hpy188III TCNNGA 2 cut(s) 83, 437
Hpy8I GTNNAC 2 cut(s) 211, 409
Hpy99I CGWCG 1 cut(s) 40
HpyAV CCTTC 1 cut(s) 49
HpyCH4III ACNGT 1 cut(s) 298
HpyCH4IV ACGT 1 cut(s) 426
HpyCH4V TGCA 2 cut(s) 203, 453
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 462
HpyF3I CTNAG 1 cut(s) 501
HpySE526I ACGT 1 cut(s) 426
Hsp92II CATG 1 cut(s) 451
HspAI GCGC 1 cut(s) 430
KroI GCCGGC 1 cut(s) 92
KroNI GCCGGC 1 cut(s) 94
Kzo9I GATC 1 cut(s) 66
LmnI GCTCC 1 cut(s) 259
LpnPI CCDG 4 cut(s) 106, 248, 299, 378
Lsp1109I GCAGC 1 cut(s) 465
LweI GCATC 4 cut(s) 163, 190, 256, 353
MaeI CTAG 2 cut(s) 41, 215
MaeII ACGT 1 cut(s) 426
MalI GATC 1 cut(s) 68
MboI GATC 1 cut(s) 66
MboII GAAGA 2 cut(s) 362, 365
MflI RGATCY 1 cut(s) 66
MlsI TGGCCA 1 cut(s) 293
MluNI TGGCCA 1 cut(s) 293
MlyI GAGTC 1 cut(s) 413
MnlI CCTC 4 cut(s) 52, 277, 284, 444
Mox20I TGGCCA 1 cut(s) 293
MroNI GCCGGC 1 cut(s) 92
MroXI GAANNNNTTC 1 cut(s) 383
MscI TGGCCA 1 cut(s) 293
MseI TTAA 1 cut(s) 120
Msp20I TGGCCA 1 cut(s) 293
MspI CCGG 1 cut(s) 93
MvnI CGCG 2 cut(s) 35, 245
MwoI GCNNNNNNNGC 2 cut(s) 115, 462
NaeI GCCGGC 1 cut(s) 94
NdeI CATATG 2 cut(s) 114, 337
NdeII GATC 1 cut(s) 66
NgoMIV GCCGGC 1 cut(s) 92
NlaIII CATG 1 cut(s) 451
PdiI GCCGGC 1 cut(s) 94
PdmI GAANNNNTTC 1 cut(s) 383
PfeI GAWTC 4 cut(s) 221, 240, 248, 486
PkrI GCNGC 2 cut(s) 343, 455
PleI GAGTC 1 cut(s) 413
PpsI GAGTC 1 cut(s) 413
PsuI RGATCY 1 cut(s) 66
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
SaqAI TTAA 1 cut(s) 120
SatI GCNGC 2 cut(s) 342, 454
Sau3AI GATC 1 cut(s) 66
SchI GAGTC 1 cut(s) 413
SetI ASST 6 cut(s) 107, 198, 216, 264, 372, 429
SfaNI GCATC 4 cut(s) 163, 190, 256, 353
SsiI CCGC 2 cut(s) 245, 341
SspMI CTAG 2 cut(s) 41, 215
StyI CCWWGG 2 cut(s) 192, 370
TaaI ACNGT 1 cut(s) 298
TaiI ACGT 1 cut(s) 429
TaqI TCGA 3 cut(s) 12, 84, 422
TatI WGTACW 1 cut(s) 504
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 4 cut(s) 221, 240, 248, 486
Tru1I TTAA 1 cut(s) 120
Tru9I TTAA 1 cut(s) 120
TscAI CASTG 1 cut(s) 301
TseI GCWGC 1 cut(s) 453
TspDTI ATGAA 2 cut(s) 101, 436
TspRI CASTG 1 cut(s) 301
XmnI GAANNNNTTC 1 cut(s) 383
XspI CTAG 2 cut(s) 41, 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.