Rh4BG259400
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001630
Physical Location & Seq
Reverse (-)
43249386 .. 43252465
3080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG259400.1

Sequence Viewer

Length: 582 bp
ATGTTCTTGTTCGACTGGTTCTACGGCGTCCTCGCATCTCTGGGCCTCTGGCAGAAGGAGGCCAAGATTCTCTTCCTCGGCCTCGACAATGCCGGAAAGACCACGCTGCTTCATATGTTGAAGGATGAGAGACTAGTGCAGCACCAGCCGACGCAGCATCCGACGTCGGAGGAATTGAGCATTGGGAAGATTAAGTTCAAGGCTTTCGATTTGGGGGGACACCAGATCGCTCGCAGAGTCTGGAAGGATTACTATGCTAAGGTGGATGCAGTAGTTTACTTGGTTGATGCATATGATAAAGATCGGTTTGCAGAGTCAAAGAAGGAGCTGGATGCACTCCTTTCAGATGAAAGCCTGGCAACTGTTCCATTTCTTGTTCTGGGCAACAAGATTGACATCCCCTATGCTGCTTCAGAAGATGAGCTGCGCTATCATCTGGGACTGAGCAATTTCACAACTGGCAAGGGAAAAGTGAATCTTGCTGATTCTAGTGTTCGGCCCCTTGAGGTCTTCATGTGCAGTATCGTGCGCAAGATGGGGTATGGAGATGGCTTCAAATGGGTCTCCCAATATATCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

193

Amino Acids

21.96

Weight (kDa)

6.97

Isoelectric Point (pI)

25.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 192 1.6e-64 ADP-ribosylation factor family
SRPRB PF09439 19 - 144 9e-08 Signal recognition particle receptor beta subunit
Roc PF08477 22 - 132 3.1e-08 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 22 - 130 1.5e-07 50S ribosome-binding GTPase
Ras PF00071 22 - 134 1.6e-06 Ras family
G-alpha PF00503 48 - 133 4.1e-09 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 167
Acc16I TGCGCA 1 cut(s) 530
AcuI CTGAAG 1 cut(s) 396
AcyI GRCGYC 2 cut(s) 27, 164
AgsI TTSAA 3 cut(s) 121, 199, 556
AhlI ACTAGT 1 cut(s) 133
AjnI CCWGG 1 cut(s) 354
AjuI GAANNNNNNNTTGG 4 cut(s) 56, 88, 165, 197
AluBI AGCT 2 cut(s) 328, 424
AluI AGCT 2 cut(s) 328, 424
Alw26I GTCTC 2 cut(s) 124, 568
AlwNI CAGNNNCTG 1 cut(s) 240
AoxI GGCC 4 cut(s) 43, 60, 79, 497
ApeKI GCWGC 5 cut(s) 106, 139, 154, 407, 424
AspLEI GCGC 2 cut(s) 429, 531
AspS9I GGNCC 2 cut(s) 43, 498
BbsI GAAGAC 1 cut(s) 502
BbvI GCAGC 5 cut(s) 93, 151, 166, 394, 411
BccI CCATC 2 cut(s) 529, 542
BceAI ACGGC 1 cut(s) 40
BciT130I CCWGG 1 cut(s) 356
BcoDI GTCTC 2 cut(s) 124, 568
BcuI ACTAGT 1 cut(s) 133
BfaI CTAG 2 cut(s) 134, 489
BisI GCNGC 5 cut(s) 107, 140, 155, 408, 425
BlsI GCNGC 5 cut(s) 108, 141, 156, 409, 426
Bme1390I CCNGG 1 cut(s) 356
BmgT120I GGNCC 2 cut(s) 43, 498
BmiI GGNNCC 1 cut(s) 500
BmrFI CCNGG 1 cut(s) 356
BmsI GCATC 5 cut(s) 44, 166, 256, 277, 322
BpiI GAAGAC 1 cut(s) 502
Bpu10I CCTNAGC 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 524
BsaBI GATNNNNATC 2 cut(s) 300, 395
BsaHI GRCGYC 2 cut(s) 27, 164
BsaI GGTCTC 1 cut(s) 568
BsaJI CCNNGG 1 cut(s) 76
Bse1I ACTGG 2 cut(s) 20, 463
Bse8I GATNNNNATC 2 cut(s) 300, 395
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 1 cut(s) 76
BseGI GGATG 5 cut(s) 130, 157, 271, 337, 396
BseJI GATNNNNATC 2 cut(s) 300, 395
BseMII CTCAG 1 cut(s) 434
BseNI ACTGG 2 cut(s) 20, 463
BseXI GCAGC 5 cut(s) 93, 151, 166, 394, 411
BsgI GTGCAG 2 cut(s) 158, 538
BshFI GGCC 4 cut(s) 45, 62, 81, 499
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 2 cut(s) 231, 453
BsmAI GTCTC 2 cut(s) 124, 568
BsmFI GGGAC 2 cut(s) 231, 453
BsnI GGCC 4 cut(s) 45, 62, 81, 499
Bso31I GGTCTC 1 cut(s) 568
Bsp143I GATC 2 cut(s) 225, 301
BspANI GGCC 4 cut(s) 45, 62, 81, 499
BspCNI CTCAG 1 cut(s) 435
BspLI GGNNCC 1 cut(s) 500
BspTNI GGTCTC 1 cut(s) 568
BsrI ACTGG 2 cut(s) 20, 463
BssECI CCNNGG 1 cut(s) 76
BssMI GATC 2 cut(s) 225, 301
BssNI GRCGYC 2 cut(s) 27, 164
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 1 cut(s) 364
Bst6I CTCTTC 1 cut(s) 77
BstACI GRCGYC 2 cut(s) 27, 164
BstC8I GCNNGC 1 cut(s) 232
BstDEI CTNAG 2 cut(s) 258, 443
BstF5I GGATG 5 cut(s) 130, 157, 271, 337, 396
BstHHI GCGC 2 cut(s) 429, 531
BstKTI GATC 2 cut(s) 228, 304
BstMAI GTCTC 2 cut(s) 124, 568
BstMBI GATC 2 cut(s) 225, 301
BstMWI GCNNNNNNNGC 2 cut(s) 145, 154
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 1 cut(s) 354
BstV1I GCAGC 5 cut(s) 93, 151, 166, 394, 411
BstV2I GAAGAC 1 cut(s) 502
BsuRI GGCC 4 cut(s) 45, 62, 81, 499
BtsCI GGATG 5 cut(s) 130, 157, 271, 337, 396
Cac8I GCNNGC 1 cut(s) 232
CaiI CAGNNNCTG 1 cut(s) 240
CfoI GCGC 2 cut(s) 429, 531
Cfr13I GGNCC 2 cut(s) 43, 498
CseI GACGC 2 cut(s) 16, 160
CviAII CATG 1 cut(s) 514
DdeI CTNAG 2 cut(s) 258, 443
DpnI GATC 2 cut(s) 227, 303
DpnII GATC 2 cut(s) 225, 301
Eam1104I CTCTTC 1 cut(s) 77
EarI CTCTTC 1 cut(s) 77
Eco31I GGTCTC 1 cut(s) 568
Eco57I CTGAAG 1 cut(s) 396
EcoRII CCWGG 1 cut(s) 354
EcoT22I ATGCAT 1 cut(s) 292
FaeI CATG 1 cut(s) 517
FaiI YATR 9 cut(s) 114, 116, 255, 292, 294, 405, 515, 543, 573
FalI AAGNNNNNCTT 4 cut(s) 56, 88, 462, 494
FaqI GGGAC 2 cut(s) 231, 453
FatI CATG 1 cut(s) 513
FauNDI CATATG 2 cut(s) 114, 292
Fnu4HI GCNGC 5 cut(s) 107, 140, 155, 408, 425
FokI GGATG 5 cut(s) 137, 144, 278, 344, 383
Fsp4HI GCNGC 5 cut(s) 107, 140, 155, 408, 425
FspBI CTAG 2 cut(s) 134, 489
FspI TGCGCA 1 cut(s) 530
GlaI GCGC 2 cut(s) 428, 530
GluI GCNGC 5 cut(s) 107, 140, 155, 408, 425
HaeIII GGCC 4 cut(s) 45, 62, 81, 499
HapII CCGG 1 cut(s) 93
HgaI GACGC 2 cut(s) 16, 160
HhaI GCGC 2 cut(s) 429, 531
Hin1I GRCGYC 2 cut(s) 27, 164
Hin1II CATG 1 cut(s) 517
Hin6I GCGC 2 cut(s) 427, 529
HinP1I GCGC 2 cut(s) 427, 529
HinfI GANTC 5 cut(s) 67, 237, 314, 475, 485
HpaII CCGG 1 cut(s) 93
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 4 cut(s) 162, 169, 346, 415
Hpy188III TCNNGA 1 cut(s) 241
Hpy8I GTNNAC 1 cut(s) 277
Hpy99I CGWCG 3 cut(s) 154, 166, 169
HpyAV CCTTC 4 cut(s) 49, 115, 238, 316
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4IV ACGT 1 cut(s) 164
HpyCH4V TGCA 6 cut(s) 139, 269, 290, 311, 335, 519
HpyF10VI GCNNNNNNNGC 2 cut(s) 145, 154
HpyF3I CTNAG 2 cut(s) 258, 443
HpySE526I ACGT 1 cut(s) 164
Hsp92I GRCGYC 2 cut(s) 27, 164
Hsp92II CATG 1 cut(s) 517
HspAI GCGC 2 cut(s) 427, 529
Kzo9I GATC 2 cut(s) 225, 301
LmnI GCTCC 1 cut(s) 325
Lsp1109I GCAGC 5 cut(s) 93, 151, 166, 394, 411
LweI GCATC 5 cut(s) 44, 166, 256, 277, 322
MaeI CTAG 2 cut(s) 134, 489
MaeII ACGT 1 cut(s) 164
MalI GATC 2 cut(s) 227, 303
MboI GATC 2 cut(s) 225, 301
MboII GAAGA 4 cut(s) 64, 199, 428, 502
MluCI AATT 2 cut(s) 173, 448
MlyI GAGTC 2 cut(s) 246, 323
MmeI TCCRAC 2 cut(s) 147, 185
MnlI CCTC 7 cut(s) 41, 52, 56, 86, 92, 163, 499
Mph1103I ATGCAT 1 cut(s) 292
MseI TTAA 1 cut(s) 192
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 356
MvaI CCWGG 1 cut(s) 356
MwoI GCNNNNNNNGC 2 cut(s) 145, 154
NdeI CATATG 2 cut(s) 114, 292
NdeII GATC 2 cut(s) 225, 301
NlaIII CATG 1 cut(s) 517
NlaIV GGNNCC 1 cut(s) 500
NmeAIII GCCGAG 1 cut(s) 57
NsbI TGCGCA 1 cut(s) 530
NsiI ATGCAT 1 cut(s) 292
PcsI WCGNNNNNNNCGW 1 cut(s) 158
PfeI GAWTC 3 cut(s) 67, 475, 485
PkrI GCNGC 5 cut(s) 108, 141, 156, 409, 426
PleI GAGTC 2 cut(s) 245, 322
PpsI GAGTC 2 cut(s) 245, 322
Psp6I CCWGG 1 cut(s) 354
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 500
PspPI GGNCC 2 cut(s) 43, 498
PstNI CAGNNNCTG 1 cut(s) 240
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 5 cut(s) 107, 140, 155, 408, 425
Sau3AI GATC 2 cut(s) 225, 301
Sau96I GGNCC 2 cut(s) 43, 498
SchI GAGTC 2 cut(s) 246, 323
ScrFI CCNGG 1 cut(s) 356
SetI ASST 5 cut(s) 167, 264, 330, 426, 510
SfaNI GCATC 5 cut(s) 44, 166, 256, 277, 322
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
SpeI ACTAGT 1 cut(s) 133
Sse9I AATT 2 cut(s) 173, 448
SspMI CTAG 2 cut(s) 134, 489
StyD4I CCNGG 1 cut(s) 354
TaaI ACNGT 1 cut(s) 364
TaiI ACGT 1 cut(s) 167
TaqI TCGA 3 cut(s) 12, 84, 207
TasI AATT 2 cut(s) 173, 448
TfiI GAWTC 3 cut(s) 67, 475, 485
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TseI GCWGC 5 cut(s) 106, 139, 154, 407, 424
TspDTI ATGAA 3 cut(s) 101, 363, 502
XspI CTAG 2 cut(s) 134, 489
ZraI GACGTC 1 cut(s) 165
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.