pycom13g13430
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001630
Physical Location & Seq
Forward (+)
9438281 .. 9440364
2084 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g13430.2

Sequence Viewer

Length: 582 bp
ATGTTTCTGCTCGACTGGTTCTACGGCGTCCTCGCTTCCCTTGGGCTGTGGCAGAAGGAGGCCAAGATTCTGTTTCTGGGTCTCGACAATGCCGGCAAGACAACCCTCCTCCACATGTTGAAGGACGAGAGATTGGTGCAGCACCAGCCGACCCAGCATCCGACTTCGGAGGAGCTGAGCATTGGGAAGATTAAGTTCAAGGCTTTCGATTTGGGCGGACACTCGATCGCTCGCCGAGTCTGGAAGGATTACTATGCTAAGGTTGATGCAGTTGTTTATCTGGTTGATGCATATGACAAGGATCGTTTTGCAGAATCAAAGAAGGAGCTGGATGCCCTCCTTTCAGATGAGAGCCTTGCAACTGTTCCATTTCTTGTGCTGGGCAACAAGATCGACATCCCTTATGCTGCTTCAGAAGATGAACTCCGATATCATTTAGGACTGACCAATTTCACAACTGGCAAGGGGAAGGTGAATCTTACAGACTCCACTGTTCGCCCTCTCGAGGTTTTCATGTGCAGTATCGTGCGCAAGATGGGGTACGGGGATGGCTTCAAGTGGGTCTCCCAATATATCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

194

Amino Acids

21.94

Weight (kDa)

6.97

Isoelectric Point (pI)

23.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 530
AciI CCGC 1 cut(s) 216
AclWI GGATC 1 cut(s) 309
AcuI CTGAAG 1 cut(s) 396
AcyI GRCGYC 1 cut(s) 27
AfaI GTAC 1 cut(s) 542
AfiI CCNNNNNNNGG 1 cut(s) 505
AflIII ACRYGT 1 cut(s) 114
AgsI TTSAA 3 cut(s) 121, 199, 556
AluBI AGCT 2 cut(s) 175, 328
AluI AGCT 2 cut(s) 175, 328
Alw26I GTCTC 2 cut(s) 86, 568
AlwI GGATC 1 cut(s) 309
Ama87I CYCGRG 1 cut(s) 503
AoxI GGCC 1 cut(s) 60
ApeKI GCWGC 2 cut(s) 139, 407
AspLEI GCGC 1 cut(s) 531
AsuHPI GGTGA 1 cut(s) 484
AvaI CYCGRG 1 cut(s) 503
BbvI GCAGC 2 cut(s) 151, 394
BccI CCATC 2 cut(s) 529, 542
BceAI ACGGC 1 cut(s) 40
BcgI CGANNNNNNTGC 2 cut(s) 373, 407
BcoDI GTCTC 2 cut(s) 86, 568
BisI GCNGC 2 cut(s) 140, 408
BlpI GCTNAGC 1 cut(s) 176
BlsI GCNGC 2 cut(s) 141, 409
BmeT110I CYCGRG 1 cut(s) 503
BmsI GCATC 4 cut(s) 166, 256, 277, 322
Bpu10I CCTNAGC 1 cut(s) 258
Bpu1102I GCTNAGC 1 cut(s) 176
BsaBI GATNNNNATC 1 cut(s) 395
BsaHI GRCGYC 1 cut(s) 27
BsaI GGTCTC 2 cut(s) 86, 568
BsaJI CCNNGG 1 cut(s) 40
Bsc4I CCNNNNNNNGG 1 cut(s) 505
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 2 cut(s) 20, 463
Bse8I GATNNNNATC 1 cut(s) 395
BseDI CCNNGG 1 cut(s) 40
BseGI GGATG 4 cut(s) 157, 337, 396, 553
BseJI GATNNNNATC 1 cut(s) 395
BseLI CCNNNNNNNGG 1 cut(s) 505
BseMII CTCAG 1 cut(s) 167
BseNI ACTGG 2 cut(s) 20, 463
BseRI GAGGAG 2 cut(s) 98, 185
BseXI GCAGC 2 cut(s) 151, 394
BseYI CCCAGC 2 cut(s) 153, 379
BsgI GTGCAG 2 cut(s) 158, 538
Bsh1285I CGRYCG 1 cut(s) 228
BshFI GGCC 1 cut(s) 62
BsiEI CGRYCG 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 503
BsiSI CCGG 1 cut(s) 93
BslI CCNNNNNNNGG 1 cut(s) 505
BsmAI GTCTC 2 cut(s) 86, 568
BsnI GGCC 1 cut(s) 62
Bso31I GGTCTC 2 cut(s) 86, 568
BsoBI CYCGRG 1 cut(s) 503
Bsp143I GATC 3 cut(s) 225, 301, 390
Bsp1720I GCTNAGC 1 cut(s) 176
BspACI CCGC 1 cut(s) 216
BspANI GGCC 1 cut(s) 62
BspCNI CTCAG 1 cut(s) 168
BspPI GGATC 1 cut(s) 309
BspTNI GGTCTC 2 cut(s) 86, 568
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 2 cut(s) 20, 463
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 1 cut(s) 40
BssMI GATC 3 cut(s) 225, 301, 390
BssNI GRCGYC 1 cut(s) 27
BssT1I CCWWGG 1 cut(s) 40
Bst4CI ACNGT 2 cut(s) 364, 493
BstACI GRCGYC 1 cut(s) 27
BstC8I GCNNGC 2 cut(s) 94, 232
BstDEI CTNAG 2 cut(s) 176, 258
BstF5I GGATG 4 cut(s) 157, 337, 396, 553
BstHHI GCGC 1 cut(s) 531
BstKTI GATC 3 cut(s) 228, 304, 393
BstMAI GTCTC 2 cut(s) 86, 568
BstMBI GATC 3 cut(s) 225, 301, 390
BstMCI CGRYCG 1 cut(s) 228
BstMWI GCNNNNNNNGC 2 cut(s) 145, 154
BstNSI RCATGY 1 cut(s) 118
BstV1I GCAGC 2 cut(s) 151, 394
BsuRI GGCC 1 cut(s) 62
BtsCI GGATG 4 cut(s) 157, 337, 396, 553
BtsIMutI CAGTG 1 cut(s) 489
Cac8I GCNNGC 2 cut(s) 94, 232
CfoI GCGC 1 cut(s) 531
Cfr10I RCCGGY 1 cut(s) 92
CseI GACGC 1 cut(s) 16
Csp6I GTAC 1 cut(s) 541
CviAII CATG 2 cut(s) 115, 514
CviJI RGCY 8 cut(s) 46, 62, 148, 175, 203, 328, 354, 552
CviKI_1 RGCY 8 cut(s) 46, 62, 148, 175, 203, 328, 354, 552
CviQI GTAC 1 cut(s) 541
DdeI CTNAG 2 cut(s) 176, 258
DpnI GATC 3 cut(s) 227, 303, 392
DpnII GATC 3 cut(s) 225, 301, 390
EciI GGCGGA 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 40
Eco31I GGTCTC 2 cut(s) 86, 568
Eco32I GATATC 1 cut(s) 431
Eco57I CTGAAG 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 503
EcoRV GATATC 1 cut(s) 431
EcoT14I CCWWGG 1 cut(s) 40
EcoT22I ATGCAT 1 cut(s) 292
ErhI CCWWGG 1 cut(s) 40
FaeI CATG 2 cut(s) 118, 517
FaiI YATR 7 cut(s) 116, 255, 292, 294, 405, 515, 573
FatI CATG 2 cut(s) 114, 513
FauNDI CATATG 1 cut(s) 292
Fnu4HI GCNGC 2 cut(s) 140, 408
FokI GGATG 4 cut(s) 144, 344, 383, 560
Fsp4HI GCNGC 2 cut(s) 140, 408
FspI TGCGCA 1 cut(s) 530
GlaI GCGC 1 cut(s) 530
GluI GCNGC 2 cut(s) 140, 408
GsaI CCCAGC 2 cut(s) 157, 383
HaeIII GGCC 1 cut(s) 62
HapII CCGG 1 cut(s) 93
HgaI GACGC 1 cut(s) 16
HhaI GCGC 1 cut(s) 531
Hin1I GRCGYC 1 cut(s) 27
Hin1II CATG 2 cut(s) 118, 517
Hin6I GCGC 1 cut(s) 529
HinP1I GCGC 1 cut(s) 529
HinfI GANTC 5 cut(s) 67, 237, 314, 475, 485
HpaII CCGG 1 cut(s) 93
HphI GGTGA 1 cut(s) 484
Hpy188I TCNGA 5 cut(s) 162, 169, 346, 415, 428
Hpy188III TCNNGA 3 cut(s) 83, 241, 503
HpyAV CCTTC 5 cut(s) 49, 115, 238, 316, 463
HpyCH4III ACNGT 2 cut(s) 364, 493
HpyCH4V TGCA 6 cut(s) 139, 269, 290, 311, 359, 519
HpyF10VI GCNNNNNNNGC 2 cut(s) 145, 154
HpyF3I CTNAG 2 cut(s) 176, 258
Hsp92I GRCGYC 1 cut(s) 27
Hsp92II CATG 2 cut(s) 118, 517
HspAI GCGC 1 cut(s) 529
KroI GCCGGC 1 cut(s) 92
KroNI GCCGGC 1 cut(s) 94
Kzo9I GATC 3 cut(s) 225, 301, 390
LmnI GCTCC 2 cut(s) 172, 325
LpnPI CCDG 9 cut(s) 62, 106, 158, 167, 226, 266, 314, 365, 444
Lsp1109I GCAGC 2 cut(s) 151, 394
LweI GCATC 4 cut(s) 166, 256, 277, 322
MalI GATC 3 cut(s) 227, 303, 392
MboI GATC 3 cut(s) 225, 301, 390
MboII GAAGA 2 cut(s) 199, 428
MluCI AATT 1 cut(s) 448
MlyI GAGTC 2 cut(s) 246, 479
MmeI TCCRAC 1 cut(s) 185
MnlI CCTC 8 cut(s) 41, 52, 116, 119, 163, 347, 499, 510
Mph1103I ATGCAT 1 cut(s) 292
MroNI GCCGGC 1 cut(s) 92
MseI TTAA 1 cut(s) 192
MspI CCGG 1 cut(s) 93
MwoI GCNNNNNNNGC 2 cut(s) 145, 154
NaeI GCCGGC 1 cut(s) 94
NdeI CATATG 1 cut(s) 292
NdeII GATC 3 cut(s) 225, 301, 390
NgoMIV GCCGGC 1 cut(s) 92
NlaIII CATG 2 cut(s) 118, 517
NmeAIII GCCGAG 1 cut(s) 260
NsbI TGCGCA 1 cut(s) 530
NsiI ATGCAT 1 cut(s) 292
NspI RCATGY 1 cut(s) 118
PaeR7I CTCGAG 1 cut(s) 503
PciI ACATGT 1 cut(s) 114
PdiI GCCGGC 1 cut(s) 94
PfeI GAWTC 3 cut(s) 67, 314, 475
PkrI GCNGC 2 cut(s) 141, 409
Ple19I CGATCG 1 cut(s) 228
PleI GAGTC 2 cut(s) 245, 479
PpsI GAGTC 2 cut(s) 245, 479
PscI ACATGT 1 cut(s) 114
PspFI CCCAGC 2 cut(s) 153, 379
PvuI CGATCG 1 cut(s) 228
RsaI GTAC 1 cut(s) 542
RsaNI GTAC 1 cut(s) 541
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 2 cut(s) 140, 408
Sau3AI GATC 3 cut(s) 225, 301, 390
SchI GAGTC 2 cut(s) 246, 479
SetI ASST 5 cut(s) 177, 264, 330, 474, 510
SfaNI GCATC 4 cut(s) 166, 256, 277, 322
Sfr274I CTCGAG 1 cut(s) 503
SlaI CTCGAG 1 cut(s) 503
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
Sse9I AATT 1 cut(s) 448
SsiI CCGC 1 cut(s) 216
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 2 cut(s) 364, 493
TaqI TCGA 6 cut(s) 12, 84, 207, 224, 393, 504
TasI AATT 1 cut(s) 448
TfiI GAWTC 3 cut(s) 67, 314, 475
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 1 cut(s) 496
TseI GCWGC 2 cut(s) 139, 407
TspDTI ATGAA 2 cut(s) 435, 502
TspRI CASTG 1 cut(s) 496
XceI RCATGY 1 cut(s) 118
XhoI CTCGAG 1 cut(s) 503
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.