pycom16g13270
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001630
Physical Location & Seq
Forward (+)
9439559 .. 9440909
1351 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g13270.3

Sequence Viewer

Length: 582 bp
ATGTTTCTGTTCGACTGGTTCTACGGCGTCCTCGCCTCCCTCGGGTTGTGGCAGAAGGAGGCCAAGATTCTGTTTCTGGGCCTCGACAATGCCGGCAAGACGACCCTCCTCCACATGCTGAAGGACGAGAGATTGGTGCAGCACCAGCCGACCCAGCATCCCACGTCCGAGGAGCTCAGCATTGGGAAGATTAAGTTCAAGGCCTTCGATTTGGGCGGACACCAGATCGCTCGCAGAGTCTGGAAAGATTACTATGCTAAGGTGGATGCAGTTGTGTACCTGGTTGATGCATACGACAAGGATCGTTTTGCAGAATCAAAGAAGGAGCTGGATGCTCTCCTTTCAGATGAGAGCCTCGCAACTGTTCCATTTCTTGTGTTGGGCAACAAGATCGACATCCCTTATGCTGCTTCAGAAGATGAACTGCGATATCATCTAGGACTGACCAATTTCACAACTGGCAAGGGGAAGTTGAATGTTACAGACTCCACTGTTCGCCCTCTCGAGGTTTTCATGTGCAGTATTGTGCGCAAGATGGGGTACGGGGATGGCTTCAAGTGGGTCTCCCAATATATCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

194

Amino Acids

22.02

Weight (kDa)

6.97

Isoelectric Point (pI)

23.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 530
AciI CCGC 1 cut(s) 216
AclWI GGATC 1 cut(s) 309
AcuI CTGAAG 2 cut(s) 140, 396
AcyI GRCGYC 1 cut(s) 27
AfaI GTAC 2 cut(s) 278, 542
AfiI CCNNNNNNNGG 2 cut(s) 42, 505
AgsI TTSAA 3 cut(s) 199, 475, 556
AjiI CACGTC 1 cut(s) 165
AjnI CCWGG 1 cut(s) 279
AluBI AGCT 2 cut(s) 175, 328
AluI AGCT 2 cut(s) 175, 328
Alw21I GWGCWC 1 cut(s) 177
Alw26I GTCTC 1 cut(s) 568
AlwI GGATC 1 cut(s) 309
AlwNI CAGNNNCTG 1 cut(s) 240
Ama87I CYCGRG 2 cut(s) 41, 503
AoxI GGCC 3 cut(s) 60, 79, 201
ApeKI GCWGC 2 cut(s) 139, 407
AspLEI GCGC 1 cut(s) 531
AspS9I GGNCC 1 cut(s) 79
AvaI CYCGRG 2 cut(s) 41, 503
BanII GRGCYC 1 cut(s) 177
Bbv12I GWGCWC 1 cut(s) 177
BbvI GCAGC 2 cut(s) 151, 394
BccI CCATC 2 cut(s) 529, 542
BceAI ACGGC 1 cut(s) 40
BcgI CGANNNNNNTGC 2 cut(s) 373, 407
BciT130I CCWGG 1 cut(s) 281
BcoDI GTCTC 1 cut(s) 568
BfaI CTAG 1 cut(s) 437
BisI GCNGC 2 cut(s) 140, 408
BlpI GCTNAGC 1 cut(s) 176
BlsI GCNGC 2 cut(s) 141, 409
Bme1390I CCNGG 1 cut(s) 281
BmeT110I CYCGRG 2 cut(s) 41, 503
BmgBI CACGTC 1 cut(s) 165
BmgT120I GGNCC 1 cut(s) 79
BmrFI CCNGG 1 cut(s) 281
BmsI GCATC 4 cut(s) 166, 256, 277, 322
Bpu10I CCTNAGC 1 cut(s) 258
Bpu1102I GCTNAGC 1 cut(s) 176
BsaBI GATNNNNATC 1 cut(s) 395
BsaHI GRCGYC 1 cut(s) 27
BsaI GGTCTC 1 cut(s) 568
BsaJI CCNNGG 2 cut(s) 40, 168
Bsc4I CCNNNNNNNGG 2 cut(s) 42, 505
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 2 cut(s) 20, 463
Bse8I GATNNNNATC 1 cut(s) 395
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 2 cut(s) 40, 168
BseGI GGATG 5 cut(s) 157, 271, 337, 396, 553
BseJI GATNNNNATC 1 cut(s) 395
BseLI CCNNNNNNNGG 2 cut(s) 42, 505
BseMII CTCAG 1 cut(s) 190
BseNI ACTGG 2 cut(s) 20, 463
BseRI GAGGAG 2 cut(s) 98, 185
BseXI GCAGC 2 cut(s) 151, 394
BseYI CCCAGC 1 cut(s) 153
BsgI GTGCAG 2 cut(s) 158, 538
BshFI GGCC 3 cut(s) 62, 81, 203
BsiHKAI GWGCWC 1 cut(s) 177
BsiHKCI CYCGRG 2 cut(s) 41, 503
BsiSI CCGG 1 cut(s) 93
BslI CCNNNNNNNGG 2 cut(s) 42, 505
BsmAI GTCTC 1 cut(s) 568
BsnI GGCC 3 cut(s) 62, 81, 203
Bso31I GGTCTC 1 cut(s) 568
BsoBI CYCGRG 2 cut(s) 41, 503
Bsp1286I GDGCHC 1 cut(s) 177
Bsp143I GATC 3 cut(s) 225, 301, 390
Bsp1720I GCTNAGC 1 cut(s) 176
BspACI CCGC 1 cut(s) 216
BspANI GGCC 3 cut(s) 62, 81, 203
BspCNI CTCAG 1 cut(s) 189
BspPI GGATC 1 cut(s) 309
BspTNI GGTCTC 1 cut(s) 568
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 2 cut(s) 20, 463
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 2 cut(s) 40, 168
BssMI GATC 3 cut(s) 225, 301, 390
BssNI GRCGYC 1 cut(s) 27
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 2 cut(s) 364, 493
BstACI GRCGYC 1 cut(s) 27
BstC8I GCNNGC 2 cut(s) 94, 232
BstDEI CTNAG 2 cut(s) 176, 258
BstF5I GGATG 5 cut(s) 157, 271, 337, 396, 553
BstHHI GCGC 1 cut(s) 531
BstKTI GATC 3 cut(s) 228, 304, 393
BstMAI GTCTC 1 cut(s) 568
BstMBI GATC 3 cut(s) 225, 301, 390
BstMWI GCNNNNNNNGC 2 cut(s) 145, 154
BstNI CCWGG 1 cut(s) 281
BstNSI RCATGY 1 cut(s) 118
BstSCI CCNGG 1 cut(s) 279
BstV1I GCAGC 2 cut(s) 151, 394
BsuRI GGCC 3 cut(s) 62, 81, 203
BtrI CACGTC 1 cut(s) 165
BtsCI GGATG 5 cut(s) 157, 271, 337, 396, 553
BtsIMutI CAGTG 1 cut(s) 489
Cac8I GCNNGC 2 cut(s) 94, 232
CaiI CAGNNNCTG 1 cut(s) 240
CfoI GCGC 1 cut(s) 531
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 1 cut(s) 79
CseI GACGC 1 cut(s) 16
CsiI ACCWGGT 1 cut(s) 279
Csp6I GTAC 2 cut(s) 277, 541
CviAII CATG 2 cut(s) 115, 514
CviJI RGCY 8 cut(s) 62, 81, 148, 175, 203, 328, 354, 552
CviKI_1 RGCY 8 cut(s) 62, 81, 148, 175, 203, 328, 354, 552
CviQI GTAC 2 cut(s) 277, 541
DdeI CTNAG 2 cut(s) 176, 258
DpnI GATC 3 cut(s) 227, 303, 392
DpnII GATC 3 cut(s) 225, 301, 390
EciI GGCGGA 1 cut(s) 231
Ecl136II GAGCTC 1 cut(s) 175
Eco147I AGGCCT 1 cut(s) 203
Eco24I GRGCYC 1 cut(s) 177
Eco31I GGTCTC 1 cut(s) 568
Eco32I GATATC 1 cut(s) 431
Eco53kI GAGCTC 1 cut(s) 175
Eco57I CTGAAG 2 cut(s) 140, 396
Eco88I CYCGRG 2 cut(s) 41, 503
EcoICRI GAGCTC 1 cut(s) 175
EcoRII CCWGG 1 cut(s) 279
EcoRV GATATC 1 cut(s) 431
EcoT22I ATGCAT 1 cut(s) 292
EcoT38I GRGCYC 1 cut(s) 177
FaeI CATG 2 cut(s) 118, 517
FaiI YATR 6 cut(s) 116, 255, 292, 405, 515, 573
FatI CATG 2 cut(s) 114, 513
Fnu4HI GCNGC 2 cut(s) 140, 408
FokI GGATG 5 cut(s) 144, 278, 344, 383, 560
FriOI GRGCYC 1 cut(s) 177
Fsp4HI GCNGC 2 cut(s) 140, 408
FspBI CTAG 1 cut(s) 437
FspI TGCGCA 1 cut(s) 530
GlaI GCGC 1 cut(s) 530
GluI GCNGC 2 cut(s) 140, 408
GsaI CCCAGC 1 cut(s) 157
HaeIII GGCC 3 cut(s) 62, 81, 203
HapII CCGG 1 cut(s) 93
HgaI GACGC 1 cut(s) 16
HhaI GCGC 1 cut(s) 531
Hin1I GRCGYC 1 cut(s) 27
Hin1II CATG 2 cut(s) 118, 517
Hin6I GCGC 1 cut(s) 529
HinP1I GCGC 1 cut(s) 529
HinfI GANTC 4 cut(s) 67, 237, 314, 485
HpaII CCGG 1 cut(s) 93
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 3 cut(s) 169, 346, 415
Hpy188III TCNNGA 2 cut(s) 241, 503
Hpy8I GTNNAC 1 cut(s) 277
HpyAV CCTTC 4 cut(s) 49, 115, 214, 316
HpyCH4III ACNGT 2 cut(s) 364, 493
HpyCH4IV ACGT 1 cut(s) 164
HpyCH4V TGCA 5 cut(s) 139, 269, 290, 311, 519
HpyF10VI GCNNNNNNNGC 2 cut(s) 145, 154
HpyF3I CTNAG 2 cut(s) 176, 258
HpySE526I ACGT 1 cut(s) 164
Hsp92I GRCGYC 1 cut(s) 27
Hsp92II CATG 2 cut(s) 118, 517
HspAI GCGC 1 cut(s) 529
KroI GCCGGC 1 cut(s) 92
KroNI GCCGGC 1 cut(s) 94
Kzo9I GATC 3 cut(s) 225, 301, 390
LmnI GCTCC 2 cut(s) 172, 325
Lsp1109I GCAGC 2 cut(s) 151, 394
LweI GCATC 4 cut(s) 166, 256, 277, 322
MabI ACCWGGT 1 cut(s) 279
MaeI CTAG 1 cut(s) 437
MaeII ACGT 1 cut(s) 164
MaeIII GTNAC 1 cut(s) 478
MalI GATC 3 cut(s) 227, 303, 392
MboI GATC 3 cut(s) 225, 301, 390
MboII GAAGA 2 cut(s) 199, 428
MhlI GDGCHC 1 cut(s) 177
MluCI AATT 1 cut(s) 448
MlyI GAGTC 2 cut(s) 246, 479
Mph1103I ATGCAT 1 cut(s) 292
MroNI GCCGGC 1 cut(s) 92
MseI TTAA 1 cut(s) 192
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 281
MvaI CCWGG 1 cut(s) 281
MwoI GCNNNNNNNGC 2 cut(s) 145, 154
NaeI GCCGGC 1 cut(s) 94
NdeII GATC 3 cut(s) 225, 301, 390
NgoMIV GCCGGC 1 cut(s) 92
NlaIII CATG 2 cut(s) 118, 517
NsbI TGCGCA 1 cut(s) 530
NsiI ATGCAT 1 cut(s) 292
NspI RCATGY 1 cut(s) 118
PaeR7I CTCGAG 1 cut(s) 503
PceI AGGCCT 1 cut(s) 203
PdiI GCCGGC 1 cut(s) 94
PfeI GAWTC 2 cut(s) 67, 314
PkrI GCNGC 2 cut(s) 141, 409
PleI GAGTC 2 cut(s) 245, 479
PpsI GAGTC 2 cut(s) 245, 479
Psp124BI GAGCTC 1 cut(s) 177
Psp6I CCWGG 1 cut(s) 279
PspFI CCCAGC 1 cut(s) 153
PspGI CCWGG 1 cut(s) 279
PspPI GGNCC 1 cut(s) 79
PstNI CAGNNNCTG 1 cut(s) 240
RsaI GTAC 2 cut(s) 278, 542
RsaNI GTAC 2 cut(s) 277, 541
SacI GAGCTC 1 cut(s) 177
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 2 cut(s) 140, 408
Sau3AI GATC 3 cut(s) 225, 301, 390
Sau96I GGNCC 1 cut(s) 79
SchI GAGTC 2 cut(s) 246, 479
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 1 cut(s) 177
SetI ASST 6 cut(s) 167, 177, 264, 282, 330, 510
SexAI ACCWGGT 1 cut(s) 279
SfaNI GCATC 4 cut(s) 166, 256, 277, 322
Sfr274I CTCGAG 1 cut(s) 503
SlaI CTCGAG 1 cut(s) 503
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
Sse9I AATT 1 cut(s) 448
SseBI AGGCCT 1 cut(s) 203
SsiI CCGC 1 cut(s) 216
SspMI CTAG 1 cut(s) 437
SstI GAGCTC 1 cut(s) 177
StuI AGGCCT 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 279
TaaI ACNGT 2 cut(s) 364, 493
TaiI ACGT 1 cut(s) 167
TaqI TCGA 5 cut(s) 12, 84, 207, 393, 504
TasI AATT 1 cut(s) 448
TfiI GAWTC 2 cut(s) 67, 314
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 1 cut(s) 496
TseI GCWGC 2 cut(s) 139, 407
TspDTI ATGAA 2 cut(s) 435, 502
TspRI CASTG 1 cut(s) 496
XceI RCATGY 1 cut(s) 118
XhoI CTCGAG 1 cut(s) 503
XspI CTAG 1 cut(s) 437
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.