RLG00000007472
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001630
Physical Location & Seq
Forward (+)
17254063 .. 17256602
2540 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007472

Sequence Viewer

Length: 522 bp
ATGTTCTTGTTCGACTGGTTCTACGGCGTCCTCGCATCTCTGGGCCTCTGGCAGAAGGAGGCCAAGATTCTGTTCCTCGGCCTCGACAATGCCGGAAAGACCACGCTGCTTCATATGTTGAAGGATGAGATTAAGTTCAAGGCCTTCGATTTGGGGGGACACCAGATCGCTCGTAGAGTCTGGAAGGATTACTATGCTAAGGTGGATGCAGTAGTTTACTTGGTTGATGCATATGACAAAGATCGGTTTGCAGAGTCAAAGAAGGAGCTGGATGCACTCCTTTCAGATGAAAGCCTGGCAACTGTTCCATTTCTTGTGCTGGGCAACAAGATTGACATCCCCTATGCTGCTTCAGAAGATGAGCTGCGCTATCATCTGGGACTGAGCAATTTCACAACTGGCAAGGGAAAAGTGAATCTTGCTGATTCTAGTGTTCGGCCCCTTGAGGTCTTCATGTGCAGTATCGTGCGCAAGATGGGGTATGGAGATGGCTTCAAATGGGTCTCCCAATATATCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

174

Amino Acids

19.69

Weight (kDa)

6.83

Isoelectric Point (pI)

25.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 43 3.6e-10 ADP-ribosylation factor family
Arf PF00025 43 - 172 1.1e-39 ADP-ribosylation factor family
G-alpha PF00503 43 - 113 1.1e-07 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 470
AcuI CTGAAG 1 cut(s) 336
AcyI GRCGYC 1 cut(s) 27
AgsI TTSAA 3 cut(s) 121, 139, 496
AjnI CCWGG 1 cut(s) 294
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AluBI AGCT 2 cut(s) 268, 364
AluI AGCT 2 cut(s) 268, 364
Alw26I GTCTC 1 cut(s) 508
AoxI GGCC 5 cut(s) 43, 60, 79, 141, 437
ApeKI GCWGC 3 cut(s) 106, 347, 364
AspLEI GCGC 2 cut(s) 369, 471
AspS9I GGNCC 2 cut(s) 43, 438
BbsI GAAGAC 1 cut(s) 442
BbvI GCAGC 3 cut(s) 93, 334, 351
BccI CCATC 2 cut(s) 469, 482
BceAI ACGGC 1 cut(s) 40
BciT130I CCWGG 1 cut(s) 296
BcoDI GTCTC 1 cut(s) 508
BfaI CTAG 1 cut(s) 429
BisI GCNGC 3 cut(s) 107, 348, 365
BlsI GCNGC 3 cut(s) 108, 349, 366
Bme1390I CCNGG 1 cut(s) 296
BmgT120I GGNCC 2 cut(s) 43, 438
BmiI GGNNCC 1 cut(s) 440
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 4 cut(s) 44, 196, 217, 262
BpiI GAAGAC 1 cut(s) 442
Bpu10I CCTNAGC 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 464
BsaBI GATNNNNATC 1 cut(s) 335
BsaHI GRCGYC 1 cut(s) 27
BsaI GGTCTC 1 cut(s) 508
BsaJI CCNNGG 1 cut(s) 76
Bse1I ACTGG 2 cut(s) 20, 403
Bse8I GATNNNNATC 1 cut(s) 335
BseBI CCWGG 1 cut(s) 296
BseDI CCNNGG 1 cut(s) 76
BseGI GGATG 4 cut(s) 130, 211, 277, 336
BseJI GATNNNNATC 1 cut(s) 335
BseMII CTCAG 1 cut(s) 374
BseNI ACTGG 2 cut(s) 20, 403
BseXI GCAGC 3 cut(s) 93, 334, 351
BseYI CCCAGC 1 cut(s) 319
BsgI GTGCAG 1 cut(s) 478
BshFI GGCC 5 cut(s) 45, 62, 81, 143, 439
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 2 cut(s) 171, 393
BsmAI GTCTC 1 cut(s) 508
BsmFI GGGAC 2 cut(s) 171, 393
BsnI GGCC 5 cut(s) 45, 62, 81, 143, 439
Bso31I GGTCTC 1 cut(s) 508
Bsp143I GATC 2 cut(s) 165, 241
BspANI GGCC 5 cut(s) 45, 62, 81, 143, 439
BspCNI CTCAG 1 cut(s) 375
BspLI GGNNCC 1 cut(s) 440
BspTNI GGTCTC 1 cut(s) 508
BsrI ACTGG 2 cut(s) 20, 403
BssECI CCNNGG 1 cut(s) 76
BssMI GATC 2 cut(s) 165, 241
BssNI GRCGYC 1 cut(s) 27
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 1 cut(s) 304
BstACI GRCGYC 1 cut(s) 27
BstDEI CTNAG 2 cut(s) 198, 383
BstF5I GGATG 4 cut(s) 130, 211, 277, 336
BstHHI GCGC 2 cut(s) 369, 471
BstKTI GATC 2 cut(s) 168, 244
BstMAI GTCTC 1 cut(s) 508
BstMBI GATC 2 cut(s) 165, 241
BstNI CCWGG 1 cut(s) 296
BstSCI CCNGG 1 cut(s) 294
BstV1I GCAGC 3 cut(s) 93, 334, 351
BstV2I GAAGAC 1 cut(s) 442
BsuRI GGCC 5 cut(s) 45, 62, 81, 143, 439
BtsCI GGATG 4 cut(s) 130, 211, 277, 336
CfoI GCGC 2 cut(s) 369, 471
Cfr13I GGNCC 2 cut(s) 43, 438
CseI GACGC 1 cut(s) 16
CviAII CATG 1 cut(s) 454
CviJI RGCY 9 cut(s) 45, 62, 81, 143, 268, 294, 364, 439, 492
CviKI_1 RGCY 9 cut(s) 45, 62, 81, 143, 268, 294, 364, 439, 492
DdeI CTNAG 2 cut(s) 198, 383
DpnI GATC 2 cut(s) 167, 243
DpnII GATC 2 cut(s) 165, 241
Eco147I AGGCCT 1 cut(s) 143
Eco31I GGTCTC 1 cut(s) 508
Eco57I CTGAAG 1 cut(s) 336
EcoRII CCWGG 1 cut(s) 294
EcoT22I ATGCAT 1 cut(s) 232
FaeI CATG 1 cut(s) 457
FaiI YATR 9 cut(s) 114, 116, 195, 232, 234, 345, 455, 483, 513
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FaqI GGGAC 2 cut(s) 171, 393
FatI CATG 1 cut(s) 453
FauNDI CATATG 2 cut(s) 114, 232
Fnu4HI GCNGC 3 cut(s) 107, 348, 365
FokI GGATG 4 cut(s) 137, 218, 284, 323
Fsp4HI GCNGC 3 cut(s) 107, 348, 365
FspBI CTAG 1 cut(s) 429
FspI TGCGCA 1 cut(s) 470
GlaI GCGC 2 cut(s) 368, 470
GluI GCNGC 3 cut(s) 107, 348, 365
GsaI CCCAGC 1 cut(s) 323
HaeIII GGCC 5 cut(s) 45, 62, 81, 143, 439
HapII CCGG 1 cut(s) 93
HgaI GACGC 1 cut(s) 16
HhaI GCGC 2 cut(s) 369, 471
Hin1I GRCGYC 1 cut(s) 27
Hin1II CATG 1 cut(s) 457
Hin6I GCGC 2 cut(s) 367, 469
HinP1I GCGC 2 cut(s) 367, 469
HinfI GANTC 5 cut(s) 67, 177, 254, 415, 425
HpaII CCGG 1 cut(s) 93
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 2 cut(s) 286, 355
Hpy188III TCNNGA 1 cut(s) 181
Hpy8I GTNNAC 1 cut(s) 217
HpyAV CCTTC 5 cut(s) 49, 115, 154, 178, 256
HpyCH4III ACNGT 1 cut(s) 304
HpyCH4V TGCA 5 cut(s) 209, 230, 251, 275, 459
HpyF3I CTNAG 2 cut(s) 198, 383
Hsp92I GRCGYC 1 cut(s) 27
Hsp92II CATG 1 cut(s) 457
HspAI GCGC 2 cut(s) 367, 469
Kzo9I GATC 2 cut(s) 165, 241
LmnI GCTCC 1 cut(s) 265
Lsp1109I GCAGC 3 cut(s) 93, 334, 351
LweI GCATC 4 cut(s) 44, 196, 217, 262
MaeI CTAG 1 cut(s) 429
MalI GATC 2 cut(s) 167, 243
MboI GATC 2 cut(s) 165, 241
MboII GAAGA 2 cut(s) 368, 442
MluCI AATT 1 cut(s) 388
MlyI GAGTC 2 cut(s) 186, 263
MnlI CCTC 6 cut(s) 41, 52, 56, 86, 92, 439
Mph1103I ATGCAT 1 cut(s) 232
MseI TTAA 1 cut(s) 132
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 296
MvaI CCWGG 1 cut(s) 296
NdeI CATATG 2 cut(s) 114, 232
NdeII GATC 2 cut(s) 165, 241
NlaIII CATG 1 cut(s) 457
NlaIV GGNNCC 1 cut(s) 440
NmeAIII GCCGAG 1 cut(s) 57
NsbI TGCGCA 1 cut(s) 470
NsiI ATGCAT 1 cut(s) 232
PceI AGGCCT 1 cut(s) 143
PfeI GAWTC 3 cut(s) 67, 415, 425
PkrI GCNGC 3 cut(s) 108, 349, 366
PleI GAGTC 2 cut(s) 185, 262
PpsI GAGTC 2 cut(s) 185, 262
Psp6I CCWGG 1 cut(s) 294
PspFI CCCAGC 1 cut(s) 319
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 1 cut(s) 440
PspPI GGNCC 2 cut(s) 43, 438
SaqAI TTAA 1 cut(s) 132
SatI GCNGC 3 cut(s) 107, 348, 365
Sau3AI GATC 2 cut(s) 165, 241
Sau96I GGNCC 2 cut(s) 43, 438
SchI GAGTC 2 cut(s) 186, 263
ScrFI CCNGG 1 cut(s) 296
SetI ASST 4 cut(s) 204, 270, 366, 450
SfaNI GCATC 4 cut(s) 44, 196, 217, 262
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse9I AATT 1 cut(s) 388
SseBI AGGCCT 1 cut(s) 143
SspMI CTAG 1 cut(s) 429
StuI AGGCCT 1 cut(s) 143
StyD4I CCNGG 1 cut(s) 294
TaaI ACNGT 1 cut(s) 304
TaqI TCGA 3 cut(s) 12, 84, 147
TasI AATT 1 cut(s) 388
TfiI GAWTC 3 cut(s) 67, 415, 425
Tru1I TTAA 1 cut(s) 132
Tru9I TTAA 1 cut(s) 132
TseI GCWGC 3 cut(s) 106, 347, 364
TspDTI ATGAA 3 cut(s) 101, 303, 442
XspI CTAG 1 cut(s) 429
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.