MD16G1157700.v1.1
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001630
Physical Location & Seq
Forward (+)
12632452 .. 12635156
2705 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1157700.v1.1.491

Sequence Viewer

Length: 582 bp
ATGTTTCTGTTCGACTGGTTCTACGGCGTCCTCGCCTCCCTCGGCCTGTGGCAGAAGGAGGCCAAGATTCTCTTTCTGGGCCTCGACAACGCCGGCAAGACGACCCTCCTCCACATGCTGAAGGACGAGAGATTGGTGCAGCACCAGCCGACCCAGCATCCCACGTCGGAGGAGCTGAGCATTGGGAAGATTAAGTTCAAGGCTTTTGATTTGGGCGGACACTCGATCGCTCGGCGAGTCTGGAAGGATTACTATGCTAAGGTTGATGCAGTGGTTTATCTGGTTGATGCATACGACAAGGATCGTTTTGCAGAATCAAAGAAGGAGCTGGATGCCCTCCTTTCAGATGAGAGCCTTGCAACGGTTCCATTTCTTGTGCTGGGCAACAAGATCGACATCCCTTATGCTGCTTCAGAAGATGAACTCCGATATCATCTAGGACTGACCAATTTCACAACTGGCAAGGGGAAGGTGAATCTTACAGACTCTACCGTCCGCCCTCTCGAGGTTTTCATGTGCAGTATTGTGCGCAAGATGGGTTACGGGGATGGCTTCAAGTGGGTCTCCCAATATATCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

194

Amino Acids

21.98

Weight (kDa)

6.97

Isoelectric Point (pI)

24.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 192 2.8e-64 ADP-ribosylation factor family
SRPRB PF09439 19 - 145 1.1e-07 Signal recognition particle receptor beta subunit
Roc PF08477 22 - 132 6.1e-08 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 22 - 130 2e-07 50S ribosome-binding GTPase
Ras PF00071 22 - 134 3.5e-06 Ras family
G-alpha PF00503 48 - 133 6.8e-09 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 491
Acc16I TGCGCA 1 cut(s) 530
AciI CCGC 2 cut(s) 216, 496
AclWI GGATC 1 cut(s) 309
AcuI CTGAAG 2 cut(s) 140, 396
AcyI GRCGYC 1 cut(s) 27
AfiI CCNNNNNNNGG 2 cut(s) 361, 505
AgsI TTSAA 2 cut(s) 199, 556
AjiI CACGTC 1 cut(s) 165
AluBI AGCT 2 cut(s) 175, 328
AluI AGCT 2 cut(s) 175, 328
Alw26I GTCTC 1 cut(s) 568
AlwI GGATC 1 cut(s) 309
Ama87I CYCGRG 1 cut(s) 503
AoxI GGCC 3 cut(s) 43, 60, 79
ApeKI GCWGC 2 cut(s) 139, 407
AspLEI GCGC 1 cut(s) 531
AspS9I GGNCC 1 cut(s) 79
AsuHPI GGTGA 1 cut(s) 484
AvaI CYCGRG 1 cut(s) 503
BbvI GCAGC 2 cut(s) 151, 394
BccI CCATC 2 cut(s) 529, 542
BceAI ACGGC 1 cut(s) 40
BcgI CGANNNNNNTGC 2 cut(s) 373, 407
BcoDI GTCTC 1 cut(s) 568
BfaI CTAG 1 cut(s) 437
BisI GCNGC 2 cut(s) 140, 408
BlpI GCTNAGC 1 cut(s) 176
BlsI GCNGC 2 cut(s) 141, 409
BmeT110I CYCGRG 1 cut(s) 503
BmgBI CACGTC 1 cut(s) 165
BmgT120I GGNCC 1 cut(s) 79
BmiI GGNNCC 1 cut(s) 366
BmsI GCATC 4 cut(s) 166, 256, 277, 322
Bpu10I CCTNAGC 1 cut(s) 258
Bpu1102I GCTNAGC 1 cut(s) 176
BsaBI GATNNNNATC 1 cut(s) 395
BsaHI GRCGYC 1 cut(s) 27
BsaI GGTCTC 1 cut(s) 568
BsaJI CCNNGG 1 cut(s) 40
Bsc4I CCNNNNNNNGG 2 cut(s) 361, 505
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 2 cut(s) 20, 463
Bse8I GATNNNNATC 1 cut(s) 395
BseDI CCNNGG 1 cut(s) 40
BseGI GGATG 4 cut(s) 157, 337, 396, 553
BseJI GATNNNNATC 1 cut(s) 395
BseLI CCNNNNNNNGG 2 cut(s) 361, 505
BseMII CTCAG 1 cut(s) 167
BseNI ACTGG 2 cut(s) 20, 463
BseRI GAGGAG 2 cut(s) 98, 185
BseXI GCAGC 2 cut(s) 151, 394
BseYI CCCAGC 2 cut(s) 153, 379
BsgI GTGCAG 2 cut(s) 158, 538
Bsh1285I CGRYCG 1 cut(s) 228
BshFI GGCC 3 cut(s) 45, 62, 81
BsiEI CGRYCG 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 503
BsiSI CCGG 1 cut(s) 93
BslI CCNNNNNNNGG 2 cut(s) 361, 505
BsmAI GTCTC 1 cut(s) 568
BsnI GGCC 3 cut(s) 45, 62, 81
Bso31I GGTCTC 1 cut(s) 568
BsoBI CYCGRG 1 cut(s) 503
Bsp143I GATC 3 cut(s) 225, 301, 390
Bsp1720I GCTNAGC 1 cut(s) 176
BspACI CCGC 2 cut(s) 216, 496
BspANI GGCC 3 cut(s) 45, 62, 81
BspCNI CTCAG 1 cut(s) 168
BspLI GGNNCC 1 cut(s) 366
BspPI GGATC 1 cut(s) 309
BspTNI GGTCTC 1 cut(s) 568
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 2 cut(s) 20, 463
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 1 cut(s) 40
BssMI GATC 3 cut(s) 225, 301, 390
BssNI GRCGYC 1 cut(s) 27
Bst4CI ACNGT 2 cut(s) 364, 493
BstACI GRCGYC 1 cut(s) 27
BstC8I GCNNGC 1 cut(s) 94
BstDEI CTNAG 2 cut(s) 176, 258
BstF5I GGATG 4 cut(s) 157, 337, 396, 553
BstHHI GCGC 1 cut(s) 531
BstKTI GATC 3 cut(s) 228, 304, 393
BstMAI GTCTC 1 cut(s) 568
BstMBI GATC 3 cut(s) 225, 301, 390
BstMCI CGRYCG 1 cut(s) 228
BstMWI GCNNNNNNNGC 2 cut(s) 145, 154
BstNSI RCATGY 1 cut(s) 118
BstV1I GCAGC 2 cut(s) 151, 394
BsuRI GGCC 3 cut(s) 45, 62, 81
BtrI CACGTC 1 cut(s) 165
BtsCI GGATG 4 cut(s) 157, 337, 396, 553
BtsI GCAGTG 1 cut(s) 276
BtsIMutI CAGTG 1 cut(s) 276
Cac8I GCNNGC 1 cut(s) 94
CfoI GCGC 1 cut(s) 531
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 1 cut(s) 79
CseI GACGC 1 cut(s) 16
CviAII CATG 2 cut(s) 115, 514
CviJI RGCY 9 cut(s) 45, 62, 81, 148, 175, 203, 328, 354, 552
CviKI_1 RGCY 9 cut(s) 45, 62, 81, 148, 175, 203, 328, 354, 552
DdeI CTNAG 2 cut(s) 176, 258
DpnI GATC 3 cut(s) 227, 303, 392
DpnII GATC 3 cut(s) 225, 301, 390
DrdI GACNNNNNNGTC 1 cut(s) 491
DseDI GACNNNNNNGTC 1 cut(s) 491
EciI GGCGGA 2 cut(s) 231, 485
Eco31I GGTCTC 1 cut(s) 568
Eco32I GATATC 1 cut(s) 431
Eco57I CTGAAG 2 cut(s) 140, 396
Eco88I CYCGRG 1 cut(s) 503
EcoRV GATATC 1 cut(s) 431
EcoT22I ATGCAT 1 cut(s) 292
FaeI CATG 2 cut(s) 118, 517
FaiI YATR 6 cut(s) 116, 255, 292, 405, 515, 573
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FatI CATG 2 cut(s) 114, 513
Fnu4HI GCNGC 2 cut(s) 140, 408
FokI GGATG 4 cut(s) 144, 344, 383, 560
Fsp4HI GCNGC 2 cut(s) 140, 408
FspBI CTAG 1 cut(s) 437
FspI TGCGCA 1 cut(s) 530
GlaI GCGC 1 cut(s) 530
GluI GCNGC 2 cut(s) 140, 408
GsaI CCCAGC 2 cut(s) 157, 383
HaeIII GGCC 3 cut(s) 45, 62, 81
HapII CCGG 1 cut(s) 93
HgaI GACGC 1 cut(s) 16
HhaI GCGC 1 cut(s) 531
Hin1I GRCGYC 1 cut(s) 27
Hin1II CATG 2 cut(s) 118, 517
Hin6I GCGC 1 cut(s) 529
HinP1I GCGC 1 cut(s) 529
HinfI GANTC 5 cut(s) 67, 237, 314, 475, 485
HpaII CCGG 1 cut(s) 93
HphI GGTGA 1 cut(s) 484
Hpy188I TCNGA 4 cut(s) 169, 346, 415, 428
Hpy188III TCNNGA 2 cut(s) 241, 503
Hpy99I CGWCG 1 cut(s) 169
HpyAV CCTTC 5 cut(s) 49, 115, 238, 316, 463
HpyCH4III ACNGT 2 cut(s) 364, 493
HpyCH4IV ACGT 1 cut(s) 164
HpyCH4V TGCA 6 cut(s) 139, 269, 290, 311, 359, 519
HpyF10VI GCNNNNNNNGC 2 cut(s) 145, 154
HpyF3I CTNAG 2 cut(s) 176, 258
HpySE526I ACGT 1 cut(s) 164
Hsp92I GRCGYC 1 cut(s) 27
Hsp92II CATG 2 cut(s) 118, 517
HspAI GCGC 1 cut(s) 529
KroI GCCGGC 1 cut(s) 92
KroNI GCCGGC 1 cut(s) 94
Kzo9I GATC 3 cut(s) 225, 301, 390
LmnI GCTCC 2 cut(s) 172, 325
Lsp1109I GCAGC 2 cut(s) 151, 394
LweI GCATC 4 cut(s) 166, 256, 277, 322
MaeI CTAG 1 cut(s) 437
MaeII ACGT 1 cut(s) 164
MaeIII GTNAC 1 cut(s) 539
MalI GATC 3 cut(s) 227, 303, 392
MboI GATC 3 cut(s) 225, 301, 390
MboII GAAGA 2 cut(s) 199, 428
MluCI AATT 1 cut(s) 448
MlyI GAGTC 2 cut(s) 246, 479
MmeI TCCRAC 1 cut(s) 147
Mph1103I ATGCAT 1 cut(s) 292
MroNI GCCGGC 1 cut(s) 92
MseI TTAA 1 cut(s) 192
MspI CCGG 1 cut(s) 93
MwoI GCNNNNNNNGC 2 cut(s) 145, 154
NaeI GCCGGC 1 cut(s) 94
NdeII GATC 3 cut(s) 225, 301, 390
NgoMIV GCCGGC 1 cut(s) 92
NlaIII CATG 2 cut(s) 118, 517
NlaIV GGNNCC 1 cut(s) 366
NmeAIII GCCGAG 2 cut(s) 21, 211
NsbI TGCGCA 1 cut(s) 530
NsiI ATGCAT 1 cut(s) 292
NspI RCATGY 1 cut(s) 118
PaeR7I CTCGAG 1 cut(s) 503
PdiI GCCGGC 1 cut(s) 94
PfeI GAWTC 3 cut(s) 67, 314, 475
PkrI GCNGC 2 cut(s) 141, 409
Ple19I CGATCG 1 cut(s) 228
PleI GAGTC 2 cut(s) 245, 479
PpsI GAGTC 2 cut(s) 245, 479
PspFI CCCAGC 2 cut(s) 153, 379
PspN4I GGNNCC 1 cut(s) 366
PspPI GGNCC 1 cut(s) 79
PvuI CGATCG 1 cut(s) 228
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 2 cut(s) 140, 408
Sau3AI GATC 3 cut(s) 225, 301, 390
Sau96I GGNCC 1 cut(s) 79
SchI GAGTC 2 cut(s) 246, 479
SetI ASST 6 cut(s) 167, 177, 264, 330, 474, 510
SfaNI GCATC 4 cut(s) 166, 256, 277, 322
Sfr274I CTCGAG 1 cut(s) 503
SlaI CTCGAG 1 cut(s) 503
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
Sse9I AATT 1 cut(s) 448
SsiI CCGC 2 cut(s) 216, 496
SspMI CTAG 1 cut(s) 437
TaaI ACNGT 2 cut(s) 364, 493
TaiI ACGT 1 cut(s) 167
TaqI TCGA 5 cut(s) 12, 84, 224, 393, 504
TasI AATT 1 cut(s) 448
TfiI GAWTC 3 cut(s) 67, 314, 475
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 1 cut(s) 276
TseI GCWGC 2 cut(s) 139, 407
TspDTI ATGAA 2 cut(s) 435, 502
TspRI CASTG 1 cut(s) 276
XceI RCATGY 1 cut(s) 118
XhoI CTCGAG 1 cut(s) 503
XspI CTAG 1 cut(s) 437
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.