Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001630

Physical Location & Seq

Genomic Coordinates Forward (+)

28716905 .. 28719519

Gene Structure 2615 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1207000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 582 bp

ATGTTCTTGTTGGATTGGTTCTACGGGGTTCTTGCATCGCTCGGTTTGTGGCAGAAGGAGGCGAAGATCTTGTTTTTGGGTCTCGACAATGCCGGCAAGACGACGTTGCTTCATATGCTCAAAGATGAGAGATTAGTTCAACACCAACCGACGCAGTATCCGACGTCCGAGGAGTTAAGCATTGGGAAAATCAAGTTCAAGGCTTTTGATTTGGGCGGGCATCAGATTGCTCGCAGAGTTTGGAAAGATTACTATGCCAAGGTGGATGCAGTTGTTTACCTGGTGGATGCTTACGATAAGGAACGATTTGCAGAATCGAAAAAGGAGCTGGATGCTCTCCTCTCTGATGAGTCATTGGCCGCTGTTCCATTCCTTGTTCTGGGAAACAAGATCGACATACCATATGCGGCCTCAGAAGAAGAGTTGCGCTTCCACCTCGGTCTGAACAACTTCACCACCGGCAAGGGCAAAGTAAACTTGCAAGACTCGAATGTCCGTCCCCTCGAGGTCTTCATGTGCAGTATCGTTCGCAAAATGGGTTATGGCGATGGCTTCCAGTGGCTTTCTCAGTACATTAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0003674 GO:0005488 GO:0005525 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0017076 GO:0019001 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0044464 GO:0071944 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04141 ko05134 map04141 map05134

Pfam Domains

Protein Families

Arf

Protein Analysis

194

Amino Acids

22.06

Weight (kDa)

6.43

Isoelectric Point (pI)

31.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arf	PF00025	8 - 192	1.1e-63	ADP-ribosylation factor family
SRPRB	PF09439	19 - 143	1.3e-07	Signal recognition particle receptor beta subunit
Roc	PF08477	22 - 132	3e-08	Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1	PF01926	22 - 129	4.9e-07	50S ribosome-binding GTPase
Ras	PF00071	22 - 134	1.3e-06	Ras family
G-alpha	PF00503	51 - 133	2.2e-08	G-protein alpha subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001630)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09180 AT1G56330
fragaria_vesca	FvH4_4g19421 FvH4_5g39351 FvH4_5g39351 FvH4_6g07290
malus_domestica	MD03G1296300.v1.1 MD04G1194800.v1.1 MD11G1315700.v1.1 MD12G1207000.v1.1 MD16G1157700.v1.1
prunus_persica	Prupe.1G122600_v2.0.a1 Prupe.1G122600_v2.0.a1 Prupe.1G122600_v2.0.a1 Prupe.6G314700_v2.0.a1 Prupe.8G271000_v2.0.a1 Prupe.8G271000_v2.0.a1
pyrus_communis	pycom03g23450 pycom04g17210 pycom11g27560 pycom13g13430 pycom16g13270
rosa_chinensis	RchiOBHm_Chr3g0456901 RchiOBHm_Chr4g0424461 RchiOBHm_Chr7g0241641
rosa_laevigata	RLG00000000558 RLG00000007472 RLG00000025269
rosa_multiflora	Rmu_sc0000133.1_g000014 Rmu_sc0003567.1_g000007 Rmu_sc0019791.1_g000003 Rmu_sc0026340.1_g000006
rosa_roxburghii	Rroxscaffold_3G00218860 Rroxscaffold_5G00366400 Rroxscaffold_6G00422390
rosa_rugosa	Rorug03G0016900 Rorug07G0345900
rosa_samantha	Rh3AG077100 Rh3BG078900 Rh3CG078800 Rh3DG080800 Rh4BG259400 Rh4CG270700 Rh4DG253900 Rh7BG471500 Rh7CG516900
rosa_wichuraiana	Rw3G006620 Rw4G021940 Rw7G042270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	491
AatII	GACGTC	1 cut(s)	167
AbsI	CCTCGAGG	1 cut(s)	503
AciI	CCGC	3 cut(s)	216, 360, 407
AcoI	YGGCCR	1 cut(s)	357
AcyI	GRCGYC	1 cut(s)	164
AfaI	GTAC	1 cut(s)	572
AfiI	CCNNNNNNNGG	1 cut(s)	379
AgsI	TTSAA	2 cut(s)	140, 199
AjnI	CCWGG	1 cut(s)	279
AluBI	AGCT	1 cut(s)	328
AluI	AGCT	1 cut(s)	328
Alw26I	GTCTC	1 cut(s)	86
Ama87I	CYCGRG	1 cut(s)	503
AoxI	GGCC	2 cut(s)	357, 408
Asp700I	GAANNNNTTC	1 cut(s)	449
AspLEI	GCGC	1 cut(s)	429
AsuHPI	GGTGA	1 cut(s)	445
AvaI	CYCGRG	1 cut(s)	503
BbsI	GAAGAC	1 cut(s)	502
BccI	CCATC	1 cut(s)	542
BciT130I	CCWGG	1 cut(s)	281
BciVI	GTATCC	1 cut(s)	168
BcoDI	GTCTC	1 cut(s)	86
BfuI	GTATCC	1 cut(s)	168
BglII	AGATCT	1 cut(s)	66
BisI	GCNGC	2 cut(s)	360, 408
BlsI	GCNGC	2 cut(s)	361, 409
Bme1390I	CCNGG	1 cut(s)	281
BmeT110I	CYCGRG	1 cut(s)	503
BmrFI	CCNGG	1 cut(s)	281
BmsI	GCATC	5 cut(s)	44, 229, 256, 277, 322
BpiI	GAAGAC	1 cut(s)	502
BsaHI	GRCGYC	1 cut(s)	164
BsaI	GGTCTC	1 cut(s)	86
BsaJI	CCNNGG	3 cut(s)	168, 258, 436
Bsc4I	CCNNNNNNNGG	1 cut(s)	379
Bse118I	RCCGGY	2 cut(s)	92, 458
Bse1I	ACTGG	1 cut(s)	556
BseBI	CCWGG	1 cut(s)	281
BseDI	CCNNGG	3 cut(s)	168, 258, 436
BseGI	GGATG	3 cut(s)	271, 292, 337
BseLI	CCNNNNNNNGG	1 cut(s)	379
BseMII	CTCAG	2 cut(s)	426, 581
BseNI	ACTGG	1 cut(s)	556
BseRI	GAGGAG	2 cut(s)	185, 329
BsgI	GTGCAG	1 cut(s)	538
BshFI	GGCC	2 cut(s)	359, 410
BsiHKCI	CYCGRG	1 cut(s)	503
BsiSI	CCGG	2 cut(s)	93, 459
BslFI	GGGAC	1 cut(s)	483
BslI	CCNNNNNNNGG	1 cut(s)	379
BsmAI	GTCTC	1 cut(s)	86
BsmFI	GGGAC	1 cut(s)	483
BsnI	GGCC	2 cut(s)	359, 410
Bso31I	GGTCTC	1 cut(s)	86
BsoBI	CYCGRG	1 cut(s)	503
Bsp143I	GATC	2 cut(s)	66, 390
BspACI	CCGC	3 cut(s)	216, 360, 407
BspANI	GGCC	2 cut(s)	359, 410
BspCNI	CTCAG	2 cut(s)	425, 580
BspTNI	GGTCTC	1 cut(s)	86
BsrFI	RCCGGY	2 cut(s)	92, 458
BsrI	ACTGG	1 cut(s)	556
BssAI	RCCGGY	2 cut(s)	92, 458
BssECI	CCNNGG	3 cut(s)	168, 258, 436
BssMI	GATC	2 cut(s)	66, 390
BssNI	GRCGYC	1 cut(s)	164
BssT1I	CCWWGG	1 cut(s)	258
Bst2UI	CCWGG	1 cut(s)	281
Bst6I	CTCTTC	1 cut(s)	414
BstACI	GRCGYC	1 cut(s)	164
BstC8I	GCNNGC	3 cut(s)	94, 218, 232
BstDEI	CTNAG	2 cut(s)	412, 567
BstF5I	GGATG	3 cut(s)	271, 292, 337
BstHHI	GCGC	1 cut(s)	429
BstKTI	GATC	2 cut(s)	69, 393
BstMAI	GTCTC	1 cut(s)	86
BstMBI	GATC	2 cut(s)	66, 390
BstMWI	GCNNNNNNNGC	1 cut(s)	115
BstNI	CCWGG	1 cut(s)	281
BstSCI	CCNGG	1 cut(s)	279
BstV2I	GAAGAC	1 cut(s)	502
BstX2I	RGATCY	1 cut(s)	66
BstYI	RGATCY	1 cut(s)	66
BsuI	GTATCC	1 cut(s)	168
BsuRI	GGCC	2 cut(s)	359, 410
BtgZI	GCGATG	2 cut(s)	21, 561
BtsCI	GGATG	3 cut(s)	271, 292, 337
BtsIMutI	CAGTG	1 cut(s)	563
Cac8I	GCNNGC	3 cut(s)	94, 218, 232
CfoI	GCGC	1 cut(s)	429
Cfr10I	RCCGGY	2 cut(s)	92, 458
CseI	GACGC	1 cut(s)	160
CsiI	ACCWGGT	1 cut(s)	279
Csp6I	GTAC	1 cut(s)	571
CviAII	CATG	1 cut(s)	514
CviJI	RGCY	6 cut(s)	203, 328, 359, 410, 552, 562
CviKI_1	RGCY	6 cut(s)	203, 328, 359, 410, 552, 562
CviQI	GTAC	1 cut(s)	571
DdeI	CTNAG	2 cut(s)	412, 567
DpnI	GATC	2 cut(s)	68, 392
DpnII	GATC	2 cut(s)	66, 390
DrdI	GACNNNNNNGTC	1 cut(s)	491
DseDI	GACNNNNNNGTC	1 cut(s)	491
EaeI	YGGCCR	1 cut(s)	357
Eam1104I	CTCTTC	1 cut(s)	414
EarI	CTCTTC	1 cut(s)	414
Eco130I	CCWWGG	1 cut(s)	258
Eco31I	GGTCTC	1 cut(s)	86
Eco88I	CYCGRG	1 cut(s)	503
EcoRII	CCWGG	1 cut(s)	279
EcoT14I	CCWWGG	1 cut(s)	258
ErhI	CCWWGG	1 cut(s)	258
FaeI	CATG	1 cut(s)	517
FaiI	YATR	8 cut(s)	114, 116, 255, 398, 403, 405, 515, 543
FaqI	GGGAC	1 cut(s)	483
FatI	CATG	1 cut(s)	513
FauI	CCCGC	1 cut(s)	209
FauNDI	CATATG	2 cut(s)	114, 403
Fnu4HI	GCNGC	2 cut(s)	360, 408
FokI	GGATG	3 cut(s)	278, 299, 344
Fsp4HI	GCNGC	2 cut(s)	360, 408
GlaI	GCGC	1 cut(s)	428
GluI	GCNGC	2 cut(s)	360, 408
HaeIII	GGCC	2 cut(s)	359, 410
HapII	CCGG	2 cut(s)	93, 459
HgaI	GACGC	1 cut(s)	160
HhaI	GCGC	1 cut(s)	429
Hin1I	GRCGYC	1 cut(s)	164
Hin1II	CATG	1 cut(s)	517
Hin6I	GCGC	1 cut(s)	427
HinP1I	GCGC	1 cut(s)	427
HinfI	GANTC	3 cut(s)	314, 350, 485
HpaII	CCGG	2 cut(s)	93, 459
HphI	GGTGA	1 cut(s)	445
Hpy166II	GTNNAC	2 cut(s)	277, 475
Hpy188I	TCNGA	6 cut(s)	162, 169, 225, 346, 415, 444
Hpy188III	TCNNGA	1 cut(s)	83
Hpy8I	GTNNAC	2 cut(s)	277, 475
Hpy99I	CGWCG	3 cut(s)	106, 154, 166
HpyAV	CCTTC	1 cut(s)	49
HpyCH4IV	ACGT	2 cut(s)	104, 164
HpyCH4V	TGCA	5 cut(s)	35, 269, 311, 481, 519
HpyF10VI	GCNNNNNNNGC	1 cut(s)	115
HpyF3I	CTNAG	2 cut(s)	412, 567
HpySE526I	ACGT	2 cut(s)	104, 164
Hsp92I	GRCGYC	1 cut(s)	164
Hsp92II	CATG	1 cut(s)	517
HspAI	GCGC	1 cut(s)	427
KroI	GCCGGC	1 cut(s)	92
KroNI	GCCGGC	1 cut(s)	94
Kzo9I	GATC	2 cut(s)	66, 390
LmnI	GCTCC	1 cut(s)	325
LpnPI	CCDG	7 cut(s)	106, 266, 293, 314, 365, 472, 569
LweI	GCATC	5 cut(s)	44, 229, 256, 277, 322
MabI	ACCWGGT	1 cut(s)	279
MaeII	ACGT	2 cut(s)	104, 164
MalI	GATC	2 cut(s)	68, 392
MboI	GATC	2 cut(s)	66, 390
MboII	GAAGA	4 cut(s)	76, 428, 431, 502
MflI	RGATCY	1 cut(s)	66
MlyI	GAGTC	2 cut(s)	359, 479
MmeI	TCCRAC	1 cut(s)	185
MnlI	CCTC	7 cut(s)	52, 163, 350, 421, 446, 499, 512
MroNI	GCCGGC	1 cut(s)	92
MroXI	GAANNNNTTC	1 cut(s)	449
MseI	TTAA	2 cut(s)	176, 576
MspA1I	CMGCKG	1 cut(s)	362
MspI	CCGG	2 cut(s)	93, 459
MspR9I	CCNGG	1 cut(s)	281
MvaI	CCWGG	1 cut(s)	281
MwoI	GCNNNNNNNGC	1 cut(s)	115
NaeI	GCCGGC	1 cut(s)	94
NdeI	CATATG	2 cut(s)	114, 403
NdeII	GATC	2 cut(s)	66, 390
NgoMIV	GCCGGC	1 cut(s)	92
NlaIII	CATG	1 cut(s)	517
PaeR7I	CTCGAG	1 cut(s)	503
PcsI	WCGNNNNNNNCGW	1 cut(s)	158
PdiI	GCCGGC	1 cut(s)	94
PdmI	GAANNNNTTC	1 cut(s)	449
PfeI	GAWTC	1 cut(s)	314
PkrI	GCNGC	2 cut(s)	361, 409
PleI	GAGTC	2 cut(s)	358, 479
PpsI	GAGTC	2 cut(s)	358, 479
Psp6I	CCWGG	1 cut(s)	279
PspGI	CCWGG	1 cut(s)	279
PspXI	VCTCGAGB	1 cut(s)	503
PsuI	RGATCY	1 cut(s)	66
RsaI	GTAC	1 cut(s)	572
RsaNI	GTAC	1 cut(s)	571
SaqAI	TTAA	2 cut(s)	176, 576
SatI	GCNGC	2 cut(s)	360, 408
Sau3AI	GATC	2 cut(s)	66, 390
SchI	GAGTC	2 cut(s)	359, 479
ScrFI	CCNGG	1 cut(s)	281
SetI	ASST	7 cut(s)	107, 167, 264, 282, 330, 438, 510
SexAI	ACCWGGT	1 cut(s)	279
SfaNI	GCATC	5 cut(s)	44, 229, 256, 277, 322
Sfr274I	CTCGAG	1 cut(s)	503
SlaI	CTCGAG	1 cut(s)	503
SmlI	CTYRAG	1 cut(s)	503
SmoI	CTYRAG	1 cut(s)	503
SsiI	CCGC	3 cut(s)	216, 360, 407
StyD4I	CCNGG	1 cut(s)	279
StyI	CCWWGG	1 cut(s)	258
TaiI	ACGT	2 cut(s)	107, 167
TaqI	TCGA	5 cut(s)	84, 317, 393, 488, 504
TaqII	GACCGA	1 cut(s)	428
TatI	WGTACW	1 cut(s)	570
TauI	GCSGC	2 cut(s)	362, 410
TfiI	GAWTC	1 cut(s)	314
Tru1I	TTAA	2 cut(s)	176, 576
Tru9I	TTAA	2 cut(s)	176, 576
TscAI	CASTG	1 cut(s)	563
TspDTI	ATGAA	2 cut(s)	101, 502
TspGWI	ACGGA	1 cut(s)	485
TspRI	CASTG	1 cut(s)	563
XhoI	CTCGAG	1 cut(s)	503
XmnI	GAANNNNTTC	1 cut(s)	449
ZraI	GACGTC	1 cut(s)	165

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.