Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0004309

Physical Location & Seq

Genomic Coordinates Reverse (-)

8716704 .. 8719942

Gene Structure 3239 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1064900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1305 bp

ATGGCCTCGCCAAACCGATCTGCGGAGAACATCGTCGACGCGACGCCGCTGTTGACCAACTCGGGCGGGTCGTCCGACGATTCGAACTCGGGGCGCCAGCGATTGGTGCGCCGACAGAGCCTGCAGCAGGCCGCGCGGTTTCTTCGCCAGGCCAGCAGCCGCCGCCTCATGCGCGAGCCGTCGATGCTGGTCCGCGAGACCGCGGCGGAGCAATTGGAGGAGAGGCAGAGTGATTGGGCGTACTCCAAGCCAGTGGTGGTTCTCGACATCATCTGGAACTTCGCCTTCGTCGTCGTGGCGCTGACGGTCCTGGTTCTGAGCCGGTACGAATTGCCGAATGTGCCCCTGAGACTGTGGATCGTGGGTTACGCTGTGCAGTGCGTGGTGCATATGGTTTGTGTTTGCGTGGAGTTTCGGAGGCGGCAGTCCCGGCAGAGAAGCCGGTTTGCGGGGGTGAATTCCGCGGAGGAAGGGGTGGGGAGTGTTGGGAATTTGGGTTCTGGGTCGAGAGAGGGGGATTTGTCATCGCAGTACGTGTCGTTGGCTGATCATATGACTGAGGAGGGTACCAGGATGCTGTTATATTTGGAGCCAGTACTGTGCAAAAACAGCGTTGCTAAACACCTGGAATCTGCAAATACAATGTTTTCGTTTATCTGGTGGATCATTGGGTTCTATTGGGTATCGGCAGGTGGTCAATCTTTAGCAAACGCCTCCCCGCAACTTTACTGGCTATGTATAATTTTCCTGGGTTTTGATGTGTTTTTTGTCGTTTTCTGTGTTGCGCTGGCATGTATCATTGGTATCGCGGTTTGTTGCTGTCTTCCGTGCATTATTGCACTTTTATATGCTGTCGCAGATCAGGTAGTTCTCATGCCCAGTTATCTCTCTTTTCTGTGTGTGGGGCTGTGTTGTTGCATCAAATTTATTGGGCCTGACAAAAATTTGATGGGAATGTGCTTGATTTTTGTTGTCGATGGAGCATCTAAGGAAGACATCGAACAGCTTCCAAAATTCAAATTCAGAAAAGTTGGTAATGAGAAAGTTGCCGGCGATGTACAAGGTGGGGGAGTAATGACTGAATGTGGTACAGATTCACCCATTGAACATGTTCTTTCTCAGGAGGATGCGGAGTGCTGCATCTGCCTTTCTTCTTATGATGACGGAGTGGAACTGAGGCAACTTCCTTGTGGCCACCATTTTCACTGTTCCTGCATTGACAAGTGGCTTTTTATCAACGCTACCTGTCCTCTCTGCAAGTACAATATCTTGAAGAGTAGCAGCCAGGATCAAGGGGAAGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0031090 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

435

Amino Acids

48.05

Weight (kDa)

5.73

Isoelectric Point (pI)

54.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	377 - 420	1e-07	RING-H2 zinc finger domain
zf-RING_2	PF13639	378 - 420	5e-14	Ring finger domain
zf-RING_11	PF17123	378 - 406	1.7e-08	RING-like zinc finger
zf-C3HC4_2	PF13923	378 - 419	6e-06	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	379 - 419	1e-05	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004309)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12760 AT1G12760 AT1G63170
fragaria_vesca	FvH4_5g01740 FvH4_5g01740 FvH4_5g01740 FvH4_5g01740
malus_domestica	MD00G1110500.v1.1 MD04G1044500.v1.1 MD04G1064900.v1.1 MD10G1187400.v1.1 MD11G1252000.v1.1 MD13G1133300.v1.1
prunus_persica	Prupe.5G092900_v2.0.a1 Prupe.5G092900_v2.0.a1 Prupe.5G092900_v2.0.a1
pyrus_communis	pycom04g05870 pycom06g07380
rosa_chinensis	RchiOBHm_Chr7g0198981
rosa_laevigata	RLG00000003827
rosa_multiflora	Rmu_co8298497.1_g000001 Rmu_sc0006266.1_g000024 Rmu_sc0007328.1_g000010
rosa_roxburghii	Rroxscaffold_3G00257250
rosa_rugosa	Rorug07G0051800 Rorug07G0051900 Rorug07G0051900
rosa_samantha	Rh7AG174900 Rh7BG178100 Rh7CG184100 Rh7DG177500
rosa_wichuraiana	Rw7G015340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	680
Acc36I	ACCTGC	1 cut(s)	680
Acc65I	GGTACC	1 cut(s)	566
AccB1I	GGYRCC	2 cut(s)	93, 566
AccB7I	CCANNNNNTGG	1 cut(s)	103
AccI	GTMKAC	1 cut(s)	36
AccII	CGCG	8 cut(s)	41, 134, 136, 174, 195, 203, 464, 809
AclWI	GGATC	3 cut(s)	365, 671, 1296
AcoI	YGGCCR	1 cut(s)	1192
AcsI	RAATTY	6 cut(s)	457, 490, 923, 943, 1013, 1019
AcyI	GRCGYC	2 cut(s)	44, 94
AfaI	GTAC	8 cut(s)	242, 326, 533, 568, 597, 1059, 1090, 1262
AfiI	CCNNNNNNNGG	4 cut(s)	22, 103, 127, 448
AflIII	ACRYGT	2 cut(s)	534, 1108
AgsI	TTSAA	3 cut(s)	1018, 1106, 1273
AjnI	CCWGG	6 cut(s)	147, 309, 569, 624, 747, 1284
AloI	GAACNNNNNNTCC	2 cut(s)	481, 513
AluBI	AGCT	1 cut(s)	1006
AluI	AGCT	1 cut(s)	1006
Alw26I	GTCTC	2 cut(s)	191, 343
AlwI	GGATC	3 cut(s)	365, 671, 1296
AlwNI	CAGNNNCTG	1 cut(s)	121
Ama87I	CYCGRG	2 cut(s)	61, 88
AoxI	GGCC	5 cut(s)	3, 129, 150, 932, 1192
ApeKI	GCWGC	4 cut(s)	124, 156, 1137, 1281
ApoI	RAATTY	6 cut(s)	457, 490, 923, 943, 1013, 1019
Asp700I	GAANNNNTTC	2 cut(s)	1005, 1110
Asp718I	GGTACC	1 cut(s)	566
AspLEI	GCGC	6 cut(s)	96, 111, 136, 174, 301, 787
AspS9I	GGNCC	3 cut(s)	190, 307, 932
AsuC2I	CCSGG	1 cut(s)	430
AsuHPI	GGTGA	2 cut(s)	466, 1089
AsuII	TTCGAA	1 cut(s)	83
AvaI	CYCGRG	2 cut(s)	61, 88
AvaII	GGWCC	2 cut(s)	190, 307
BaeGI	GKGCMC	1 cut(s)	345
BalI	TGGCCA	1 cut(s)	1194
BanI	GGYRCC	2 cut(s)	93, 566
BbsI	GAAGAC	2 cut(s)	815, 999
BbvI	GCAGC	4 cut(s)	136, 168, 1124, 1293
BccI	CCATC	2 cut(s)	943, 971
BceAI	ACGGC	1 cut(s)	163
BciT130I	CCWGG	6 cut(s)	149, 311, 571, 626, 749, 1286
BclI	TGATCA	1 cut(s)	547
BcnI	CCSGG	1 cut(s)	430
BcoDI	GTCTC	2 cut(s)	191, 343
BfmI	CTRYAG	1 cut(s)	122
BfoI	RGCGCY	2 cut(s)	97, 302
BfuAI	ACCTGC	1 cut(s)	680
BmcAI	AGTACT	1 cut(s)	597
Bme1390I	CCNGG	7 cut(s)	149, 311, 430, 571, 626, 749, 1286
Bme18I	GGWCC	2 cut(s)	190, 307
BmeT110I	CYCGRG	2 cut(s)	61, 88
BmgT120I	GGNCC	3 cut(s)	190, 307, 932
BmiI	GGNNCC	3 cut(s)	95, 568, 591
BmrFI	CCNGG	7 cut(s)	149, 311, 430, 571, 626, 749, 1286
BmrI	ACTGGG	1 cut(s)	873
BmsI	GCATC	6 cut(s)	174, 564, 927, 992, 1117, 1149
BmuI	ACTGGG	1 cut(s)	873
BpiI	GAAGAC	2 cut(s)	815, 999
Bpu14I	TTCGAA	1 cut(s)	83
BpuMI	CCSGG	1 cut(s)	430
BsaAI	YACGTR	1 cut(s)	535
BsaHI	GRCGYC	2 cut(s)	44, 94
BsaI	GGTCTC	1 cut(s)	191
BsaJI	CCNNGG	3 cut(s)	201, 462, 748
Bsc4I	CCNNNNNNNGG	4 cut(s)	22, 103, 127, 448
Bse118I	RCCGGY	3 cut(s)	321, 441, 1049
Bse1I	ACTGG	4 cut(s)	251, 593, 734, 879
BseBI	CCWGG	6 cut(s)	149, 311, 571, 626, 749, 1286
BseDI	CCNNGG	3 cut(s)	201, 462, 748
BseGI	GGATG	2 cut(s)	579, 1132
BseLI	CCNNNNNNNGG	4 cut(s)	22, 103, 127, 448
BseMII	CTCAG	5 cut(s)	308, 338, 549, 1133, 1166
BseNI	ACTGG	4 cut(s)	251, 593, 734, 879
BseRI	GAGGAG	2 cut(s)	233, 575
BseSI	GKGCMC	1 cut(s)	345
BseXI	GCAGC	4 cut(s)	136, 168, 1124, 1293
BsgI	GTGCAG	1 cut(s)	395
Bsh1236I	CGCG	8 cut(s)	41, 134, 136, 174, 195, 203, 464, 809
BshFI	GGCC	5 cut(s)	5, 131, 152, 934, 1194
BshNI	GGYRCC	2 cut(s)	93, 566
BsiHKCI	CYCGRG	2 cut(s)	61, 88
BsiSI	CCGG	4 cut(s)	322, 430, 442, 1050
BslFI	GGGAC	1 cut(s)	412
BslI	CCNNNNNNNGG	4 cut(s)	22, 103, 127, 448
BsmAI	GTCTC	2 cut(s)	191, 343
BsmFI	GGGAC	1 cut(s)	412
BsnI	GGCC	5 cut(s)	5, 131, 152, 934, 1194
Bso31I	GGTCTC	1 cut(s)	191
BsoBI	CYCGRG	2 cut(s)	61, 88
Bsp119I	TTCGAA	1 cut(s)	83
Bsp1286I	GDGCHC	1 cut(s)	345
Bsp1407I	TGTACA	1 cut(s)	1057
Bsp143I	GATC	6 cut(s)	17, 357, 547, 663, 859, 1288
BspANI	GGCC	5 cut(s)	5, 131, 152, 934, 1194
BspCNI	CTCAG	5 cut(s)	309, 339, 550, 1132, 1167
BspFNI	CGCG	8 cut(s)	41, 134, 136, 174, 195, 203, 464, 809
BspLI	GGNNCC	3 cut(s)	95, 568, 591
BspMAI	CTGCAG	1 cut(s)	126
BspMI	ACCTGC	1 cut(s)	680
BspPI	GGATC	3 cut(s)	365, 671, 1296
BspT104I	TTCGAA	1 cut(s)	83
BspT107I	GGYRCC	2 cut(s)	93, 566
BspTNI	GGTCTC	1 cut(s)	191
BsrFI	RCCGGY	3 cut(s)	321, 441, 1049
BsrGI	TGTACA	1 cut(s)	1057
BsrI	ACTGG	4 cut(s)	251, 593, 734, 879
BssAI	RCCGGY	3 cut(s)	321, 441, 1049
BssECI	CCNNGG	3 cut(s)	201, 462, 748
BssMI	GATC	6 cut(s)	17, 357, 547, 663, 859, 1288
BssNI	GRCGYC	2 cut(s)	44, 94
Bst2UI	CCWGG	6 cut(s)	149, 311, 571, 626, 749, 1286
Bst4CI	ACNGT	4 cut(s)	307, 354, 600, 1208
Bst6I	CTCTTC	1 cut(s)	1268
BstACI	GRCGYC	2 cut(s)	44, 94
BstAUI	TGTACA	1 cut(s)	1057
BstBAI	YACGTR	1 cut(s)	535
BstBI	TTCGAA	1 cut(s)	83
BstC8I	GCNNGC	7 cut(s)	98, 122, 129, 154, 176, 789, 1051
BstDEI	CTNAG	6 cut(s)	317, 347, 558, 987, 1119, 1175
BstDSI	CCRYGG	2 cut(s)	201, 462
BstENI	CCTNNNNNAGG	1 cut(s)	125
BstF5I	GGATG	2 cut(s)	579, 1132
BstFNI	CGCG	8 cut(s)	41, 134, 136, 174, 195, 203, 464, 809
BstH2I	RGCGCY	2 cut(s)	97, 302
BstHHI	GCGC	6 cut(s)	96, 111, 136, 174, 301, 787
BstKTI	GATC	6 cut(s)	20, 360, 550, 666, 862, 1291
BstMAI	GTCTC	2 cut(s)	191, 343
BstMBI	GATC	6 cut(s)	17, 357, 547, 663, 859, 1288
BstNI	CCWGG	6 cut(s)	149, 311, 571, 626, 749, 1286
BstNSI	RCATGY	2 cut(s)	795, 1112
BstSCI	CCNGG	7 cut(s)	147, 309, 428, 569, 624, 747, 1284
BstSFI	CTRYAG	1 cut(s)	122
BstSLI	GKGCMC	1 cut(s)	345
BstUI	CGCG	8 cut(s)	41, 134, 136, 174, 195, 203, 464, 809
BstV1I	GCAGC	4 cut(s)	136, 168, 1124, 1293
BstV2I	GAAGAC	2 cut(s)	815, 999
BstXI	CCANNNNNNTGG	1 cut(s)	253
BsuRI	GGCC	5 cut(s)	5, 131, 152, 934, 1194
BtgI	CCRYGG	2 cut(s)	201, 462
BtgZI	GCGATG	2 cut(s)	510, 1068
BtsCI	GGATG	2 cut(s)	579, 1132
BtsI	GCAGTG	1 cut(s)	383
BtsIMutI	CAGTG	3 cut(s)	258, 383, 1204
BveI	ACCTGC	1 cut(s)	680
Cac8I	GCNNGC	7 cut(s)	98, 122, 129, 154, 176, 789, 1051
CaiI	CAGNNNCTG	1 cut(s)	121
CfoI	GCGC	6 cut(s)	96, 111, 136, 174, 301, 787
Cfr10I	RCCGGY	3 cut(s)	321, 441, 1049
Cfr13I	GGNCC	3 cut(s)	190, 307, 932
Cfr42I	CCGCGG	2 cut(s)	204, 465
CseI	GACGC	2 cut(s)	47, 52
Csp6I	GTAC	8 cut(s)	241, 325, 532, 567, 596, 1058, 1089, 1261
CviAII	CATG	4 cut(s)	169, 792, 874, 1109
CviQI	GTAC	8 cut(s)	241, 325, 532, 567, 596, 1058, 1089, 1261
DdeI	CTNAG	6 cut(s)	317, 347, 558, 987, 1119, 1175
DinI	GGCGCC	1 cut(s)	95
DpnI	GATC	6 cut(s)	19, 359, 549, 665, 861, 1290
DpnII	GATC	6 cut(s)	17, 357, 547, 663, 859, 1288
EaeI	YGGCCR	1 cut(s)	1192
Eam1104I	CTCTTC	1 cut(s)	1268
EarI	CTCTTC	1 cut(s)	1268
EciI	GGCGGA	1 cut(s)	221
Eco31I	GGTCTC	1 cut(s)	191
Eco47I	GGWCC	2 cut(s)	190, 307
Eco88I	CYCGRG	2 cut(s)	61, 88
EcoNI	CCTNNNNNAGG	1 cut(s)	125
EcoRI	GAATTC	1 cut(s)	457
EcoRII	CCWGG	6 cut(s)	147, 309, 569, 624, 747, 1284
EgeI	GGCGCC	1 cut(s)	95
EheI	GGCGCC	1 cut(s)	95
FaeI	CATG	4 cut(s)	172, 795, 877, 1112
FaqI	GGGAC	1 cut(s)	412
FatI	CATG	4 cut(s)	168, 791, 873, 1108
FauI	CCCGC	3 cut(s)	59, 442, 726
FauNDI	CATATG	2 cut(s)	390, 552
FbaI	TGATCA	1 cut(s)	547
FblI	GTMKAC	1 cut(s)	36
FokI	GGATG	2 cut(s)	586, 1139
GlaI	GCGC	6 cut(s)	95, 110, 135, 173, 300, 786
HaeII	RGCGCY	2 cut(s)	97, 302
HaeIII	GGCC	5 cut(s)	5, 131, 152, 934, 1194
HapII	CCGG	4 cut(s)	322, 430, 442, 1050
HgaI	GACGC	2 cut(s)	47, 52
HhaI	GCGC	6 cut(s)	96, 111, 136, 174, 301, 787
Hin1I	GRCGYC	2 cut(s)	44, 94
Hin1II	CATG	4 cut(s)	172, 795, 877, 1112
Hin6I	GCGC	6 cut(s)	94, 109, 134, 172, 299, 785
HinP1I	GCGC	6 cut(s)	94, 109, 134, 172, 299, 785
HincII	GTYRAC	2 cut(s)	37, 54
HindII	GTYRAC	2 cut(s)	37, 54
HinfI	GANTC	3 cut(s)	80, 629, 1094
HpaII	CCGG	4 cut(s)	322, 430, 442, 1050
HphI	GGTGA	2 cut(s)	466, 1089
Hpy166II	GTNNAC	2 cut(s)	37, 54
Hpy188I	TCNGA	4 cut(s)	76, 318, 417, 1025
Hpy188III	TCNNGA	5 cut(s)	263, 274, 507, 1121, 1270
Hpy8I	GTNNAC	2 cut(s)	37, 54
Hpy99I	CGWCG	7 cut(s)	38, 41, 46, 80, 184, 293, 296
HpyAV	CCTTC	2 cut(s)	295, 464
HpyCH4III	ACNGT	4 cut(s)	307, 354, 600, 1208
HpyCH4IV	ACGT	1 cut(s)	534
HpyF3I	CTNAG	6 cut(s)	317, 347, 558, 987, 1119, 1175
HpySE526I	ACGT	1 cut(s)	534
Hsp92I	GRCGYC	2 cut(s)	44, 94
Hsp92II	CATG	4 cut(s)	172, 795, 877, 1112
HspAI	GCGC	6 cut(s)	94, 109, 134, 172, 299, 785
KasI	GGCGCC	1 cut(s)	93
KpnI	GGTACC	1 cut(s)	570
KroI	GCCGGC	1 cut(s)	1049
KroNI	GCCGGC	1 cut(s)	1051
Ksp22I	TGATCA	1 cut(s)	547
KspI	CCGCGG	2 cut(s)	204, 465
Kzo9I	GATC	6 cut(s)	17, 357, 547, 663, 859, 1288
LmnI	GCTCC	3 cut(s)	208, 589, 980
Lsp1109I	GCAGC	4 cut(s)	136, 168, 1124, 1293
LweI	GCATC	6 cut(s)	174, 564, 927, 992, 1117, 1149
MaeII	ACGT	1 cut(s)	534
MaeIII	GTNAC	1 cut(s)	365
MalI	GATC	6 cut(s)	19, 359, 549, 665, 861, 1290
MboI	GATC	6 cut(s)	17, 357, 547, 663, 859, 1288
MboII	GAAGA	5 cut(s)	134, 815, 1004, 1143, 1285
MfeI	CAATTG	1 cut(s)	212
MhlI	GDGCHC	1 cut(s)	345
MlsI	TGGCCA	1 cut(s)	1194
MluCI	AATT	9 cut(s)	212, 329, 457, 490, 741, 923, 943, 1013, 1019
MluNI	TGGCCA	1 cut(s)	1194
Mly113I	GGCGCC	1 cut(s)	94
MmeI	TCCRAC	1 cut(s)	99
Mox20I	TGGCCA	1 cut(s)	1194
MroNI	GCCGGC	1 cut(s)	1049
MroXI	GAANNNNTTC	2 cut(s)	1005, 1110
MscI	TGGCCA	1 cut(s)	1194
MseI	TTAA	1 cut(s)	1303
Msp20I	TGGCCA	1 cut(s)	1194
MspA1I	CMGCKG	3 cut(s)	49, 203, 464
MspI	CCGG	4 cut(s)	322, 430, 442, 1050
MspR9I	CCNGG	7 cut(s)	149, 311, 430, 571, 626, 749, 1286
MunI	CAATTG	1 cut(s)	212
MvaI	CCWGG	6 cut(s)	149, 311, 571, 626, 749, 1286
MvnI	CGCG	8 cut(s)	41, 134, 136, 174, 195, 203, 464, 809
NaeI	GCCGGC	1 cut(s)	1051
NarI	GGCGCC	1 cut(s)	94
NciI	CCSGG	1 cut(s)	430
NdeI	CATATG	2 cut(s)	390, 552
NdeII	GATC	6 cut(s)	17, 357, 547, 663, 859, 1288
NgoMIV	GCCGGC	1 cut(s)	1049
NlaIII	CATG	4 cut(s)	172, 795, 877, 1112
NlaIV	GGNNCC	3 cut(s)	95, 568, 591
NspI	RCATGY	2 cut(s)	795, 1112
NspV	TTCGAA	1 cut(s)	83
PaqCI	CACCTGC	1 cut(s)	680
PciI	ACATGT	1 cut(s)	1108
PcsI	WCGNNNNNNNCGW	2 cut(s)	68, 288
PdiI	GCCGGC	1 cut(s)	1051
PdmI	GAANNNNTTC	2 cut(s)	1005, 1110
PfeI	GAWTC	3 cut(s)	80, 629, 1094
PflMI	CCANNNNNTGG	1 cut(s)	103
PluTI	GGCGCC	1 cut(s)	97
Ppu21I	YACGTR	1 cut(s)	535
PscI	ACATGT	1 cut(s)	1108
Psp6I	CCWGG	6 cut(s)	147, 309, 569, 624, 747, 1284
PspGI	CCWGG	6 cut(s)	147, 309, 569, 624, 747, 1284
PspN4I	GGNNCC	3 cut(s)	95, 568, 591
PspPI	GGNCC	3 cut(s)	190, 307, 932
PstI	CTGCAG	1 cut(s)	126
PstNI	CAGNNNCTG	1 cut(s)	121
RsaI	GTAC	8 cut(s)	242, 326, 533, 568, 597, 1059, 1090, 1262
RsaNI	GTAC	8 cut(s)	241, 325, 532, 567, 596, 1058, 1089, 1261
SacII	CCGCGG	2 cut(s)	204, 465
SalI	GTCGAC	1 cut(s)	35
SaqAI	TTAA	1 cut(s)	1303
Sau3AI	GATC	6 cut(s)	17, 357, 547, 663, 859, 1288
Sau96I	GGNCC	3 cut(s)	190, 307, 932
ScaI	AGTACT	1 cut(s)	597
ScrFI	CCNGG	7 cut(s)	149, 311, 430, 571, 626, 749, 1286
SduI	GDGCHC	1 cut(s)	345
SetI	ASST	7 cut(s)	537, 627, 694, 867, 1008, 1066, 1247
SfaNI	GCATC	6 cut(s)	174, 564, 927, 992, 1117, 1149
SfcI	CTRYAG	1 cut(s)	122
SfoI	GGCGCC	1 cut(s)	95
Sfr303I	CCGCGG	2 cut(s)	204, 465
SfuI	TTCGAA	1 cut(s)	83
SgrBI	CCGCGG	2 cut(s)	204, 465
SgrDI	CGTCGACG	1 cut(s)	35
SinI	GGWCC	2 cut(s)	190, 307
Sse9I	AATT	9 cut(s)	212, 329, 457, 490, 741, 923, 943, 1013, 1019
SspDI	GGCGCC	1 cut(s)	93
StyD4I	CCNGG	7 cut(s)	147, 309, 428, 569, 624, 747, 1284
TaaI	ACNGT	4 cut(s)	307, 354, 600, 1208
TaiI	ACGT	1 cut(s)	537
TaqI	TCGA	7 cut(s)	36, 83, 182, 264, 506, 975, 999
TasI	AATT	9 cut(s)	212, 329, 457, 490, 741, 923, 943, 1013, 1019
TatI	WGTACW	3 cut(s)	595, 1057, 1260
TauI	GCSGC	6 cut(s)	49, 134, 162, 165, 206, 424
TfiI	GAWTC	3 cut(s)	80, 629, 1094
Tru1I	TTAA	1 cut(s)	1303
Tru9I	TTAA	1 cut(s)	1303
TscAI	CASTG	3 cut(s)	258, 383, 1211
TseI	GCWGC	4 cut(s)	124, 156, 1137, 1281
TspGWI	ACGGA	2 cut(s)	816, 1179
TspRI	CASTG	3 cut(s)	258, 383, 1211
Van91I	CCANNNNNTGG	1 cut(s)	103
VpaK11BI	GGWCC	2 cut(s)	190, 307
XagI	CCTNNNNNAGG	1 cut(s)	125
XapI	RAATTY	6 cut(s)	457, 490, 923, 943, 1013, 1019
XceI	RCATGY	2 cut(s)	795, 1112
XcmI	CCANNNNNNNNNTGG	1 cut(s)	253
XmiI	GTMKAC	1 cut(s)	36
XmnI	GAANNNNTTC	2 cut(s)	1005, 1110
ZrmI	AGTACT	1 cut(s)	597

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.